Rh6AG273200

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002266
Physical Location & Seq
Forward (+)
46747599 .. 46751216
3618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG273200.1

Sequence Viewer

Length: 804 bp
ATGTATTATTTCCAGCAATTTACTGTTACAAAATATTCCCTTGAGAATACCAGCATGTCGAGGGACCCGGCTACTGCTGAGAGGGTTTTGCTTGACCTGCTTGGCACTGTTGATGCCAGTATCATGCAGGCGTATACTGACAGTGACATAAGGCAGAACTTAGCCCACCATGCCCTTGCTTCCTCCCTCCACTTAGCCATGTGGCTGAGGAAAACTGGAGATGTTTCGAGGCAGAGTGATGAAGCCATAGCTCGTGTTGCAGAGCTGGAGGAAAGAGTTAGGAGGTTTGAAAAGCTCTTTGTGAAACAGGAGGCTGAAAATGAGATTCTGAGCCTAGAGGTCGACGAGTACTGTAACATGAAGGAGGAGTACGAGTCTACGATTAAGAGTCTATCAAATGAGGTTAACCAGCAGAAGCATGTGACTGTGGTCTTGGAGAGTAAGTTCAAGAATATGGAGGCCATGCACCAGGAGAAGGCTACTCGTCTGGAAAAGGCCTTGAAGCAGCTTAACGACGCCAACATGCTGCTTGCTGAGGTGAGTGAAGGTCTTGGTGTGGCCAGGGAACCAGGGGCCAGAGATCCTAGACATTTCTGGACGGTTTGCATTCACTGCAAAGTCCAGTATCAGTATATCAGGATCTATCAGAATCGCAGGCTTCACTGCCCCAATTGTCGAAATGCTTTTCTTGCTATAGAAACTGTTTCATCTCCAAAGTCCTATCCATGGAGTAGCCCTTCATGGGTGAAGCCTGAAGAAGATGGACAGGGTGCAGTCAAAGTGGAGGCTCAGGATGCTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

267

Amino Acids

30.69

Weight (kDa)

5.82

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_20 PF23551 198 - 232 2.4e-14 Zinc beta-ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 105
AccI GTMKAC 3 cut(s) 134, 342, 377
AclWI GGATC 2 cut(s) 575, 647
AcoI YGGCCR 1 cut(s) 558
AcuI CTGAAG 1 cut(s) 774
AcyI GRCGYC 1 cut(s) 516
AfaI GTAC 2 cut(s) 350, 371
AfiI CCNNNNNNNGG 3 cut(s) 475, 726, 742
AgsI TTSAA 3 cut(s) 290, 448, 502
AjnI CCWGG 3 cut(s) 468, 560, 568
AloI GAACNNNNNNTCC 2 cut(s) 428, 460
AluBI AGCT 4 cut(s) 251, 265, 295, 508
AluI AGCT 4 cut(s) 251, 265, 295, 508
AlwI GGATC 2 cut(s) 575, 647
AoxI GGCC 4 cut(s) 459, 495, 558, 573
ApeKI GCWGC 2 cut(s) 505, 526
AspS9I GGNCC 2 cut(s) 64, 573
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 550, 757
AvaII GGWCC 1 cut(s) 64
BalI TGGCCA 1 cut(s) 560
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BauI CACGAG 1 cut(s) 252
BbvCI CCTCAGC 2 cut(s) 206, 534
BbvI GCAGC 2 cut(s) 513, 517
BccI CCATC 1 cut(s) 755
BciT130I CCWGG 3 cut(s) 470, 562, 570
BcnI CCSGG 1 cut(s) 68
BfaI CTAG 2 cut(s) 335, 585
BfmI CTRYAG 1 cut(s) 693
BfuAI ACCTGC 1 cut(s) 105
BisI GCNGC 2 cut(s) 506, 527
BlsI GCNGC 2 cut(s) 507, 528
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 4 cut(s) 68, 470, 562, 570
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 64, 573
BmiI GGNNCC 4 cut(s) 65, 66, 567, 574
BmrFI CCNGG 4 cut(s) 68, 470, 562, 570
BmsI GCATC 2 cut(s) 103, 784
BoxI GACNNNNGTC 1 cut(s) 428
BpmI CTGGAG 2 cut(s) 237, 287
Bpu10I CCTNAGC 3 cut(s) 206, 534, 789
BpuEI CTTGAG 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 68
BsaHI GRCGYC 1 cut(s) 516
BsaJI CCNNGG 3 cut(s) 561, 569, 725
BsaXI ACNNNNNCTCC 4 cut(s) 359, 389, 428, 458
Bsc4I CCNNNNNNNGG 3 cut(s) 475, 726, 742
Bse1I ACTGG 3 cut(s) 117, 220, 622
BseBI CCWGG 3 cut(s) 470, 562, 570
BseDI CCNNGG 3 cut(s) 561, 569, 725
BseGI GGATG 1 cut(s) 799
BseLI CCNNNNNNNGG 3 cut(s) 475, 726, 742
BseMII CTCAG 5 cut(s) 69, 197, 320, 525, 803
BseNI ACTGG 3 cut(s) 117, 220, 622
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 2 cut(s) 513, 517
BsgI GTGCAG 1 cut(s) 792
BshFI GGCC 4 cut(s) 461, 497, 560, 575
BsiSI CCGG 1 cut(s) 68
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 475, 726, 742
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 606
BsnI GGCC 4 cut(s) 461, 497, 560, 575
Bsp143I GATC 2 cut(s) 580, 639
Bsp19I CCATGG 1 cut(s) 725
BspANI GGCC 4 cut(s) 461, 497, 560, 575
BspCNI CTCAG 5 cut(s) 70, 198, 321, 526, 802
BspLI GGNNCC 4 cut(s) 65, 66, 567, 574
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 2 cut(s) 575, 647
BsrI ACTGG 3 cut(s) 117, 220, 622
BssECI CCNNGG 3 cut(s) 561, 569, 725
BssMI GATC 2 cut(s) 580, 639
BssNAI GTATAC 1 cut(s) 135
BssNI GRCGYC 1 cut(s) 516
BssSI CACGAG 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 725
Bst1107I GTATAC 1 cut(s) 135
Bst2BI CACGAG 1 cut(s) 252
Bst2UI CCWGG 3 cut(s) 470, 562, 570
Bst4CI ACNGT 7 cut(s) 25, 109, 143, 353, 427, 601, 703
BstACI GRCGYC 1 cut(s) 516
BstAPI GCANNNNNTGC 1 cut(s) 612
BstC8I GCNNGC 3 cut(s) 129, 531, 656
BstDEI CTNAG 7 cut(s) 78, 160, 193, 206, 329, 534, 789
BstDSI CCRYGG 1 cut(s) 725
BstF5I GGATG 1 cut(s) 799
BstKTI GATC 2 cut(s) 583, 642
BstMBI GATC 2 cut(s) 580, 639
BstMWI GCNNNNNNNGC 6 cut(s) 97, 170, 257, 612, 689, 794
BstNI CCWGG 3 cut(s) 470, 562, 570
BstNSI RCATGY 3 cut(s) 58, 422, 526
BstPAI GACNNNNGTC 1 cut(s) 428
BstSCI CCNGG 4 cut(s) 66, 468, 560, 568
BstSFI CTRYAG 1 cut(s) 693
BstV1I GCAGC 2 cut(s) 513, 517
BstX2I RGATCY 2 cut(s) 580, 639
BstYI RGATCY 2 cut(s) 580, 639
BstZ17I GTATAC 1 cut(s) 135
BsuRI GGCC 4 cut(s) 461, 497, 560, 575
BtgI CCRYGG 1 cut(s) 725
BtsCI GGATG 1 cut(s) 799
BtsI GCAGTG 2 cut(s) 610, 661
BtsIMutI CAGTG 4 cut(s) 105, 148, 610, 661
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 3 cut(s) 129, 531, 656
Cfr13I GGNCC 2 cut(s) 64, 573
CseI GACGC 1 cut(s) 524
Csp6I GTAC 2 cut(s) 349, 370
CviQI GTAC 2 cut(s) 349, 370
DdeI CTNAG 7 cut(s) 78, 160, 193, 206, 329, 534, 789
DpnI GATC 2 cut(s) 582, 641
DpnII GATC 2 cut(s) 580, 639
EaeI YGGCCR 1 cut(s) 558
Eco130I CCWWGG 1 cut(s) 725
Eco147I AGGCCT 1 cut(s) 497
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 774
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 3 cut(s) 468, 560, 568
EcoT14I CCWWGG 1 cut(s) 725
ErhI CCWWGG 1 cut(s) 725
FaqI GGGAC 1 cut(s) 77
FblI GTMKAC 3 cut(s) 134, 342, 377
Fnu4HI GCNGC 2 cut(s) 506, 527
Fsp4HI GCNGC 2 cut(s) 506, 527
FspBI CTAG 2 cut(s) 335, 585
GluI GCNGC 2 cut(s) 506, 527
GsuI CTGGAG 2 cut(s) 237, 287
HaeIII GGCC 4 cut(s) 461, 497, 560, 575
HapII CCGG 1 cut(s) 68
HgaI GACGC 1 cut(s) 524
Hin1I GRCGYC 1 cut(s) 516
HincII GTYRAC 2 cut(s) 343, 406
HindII GTYRAC 2 cut(s) 343, 406
HinfI GANTC 4 cut(s) 325, 374, 388, 649
HpaI GTTAAC 1 cut(s) 406
HpaII CCGG 1 cut(s) 68
HphI GGTGA 2 cut(s) 550, 757
Hpy166II GTNNAC 4 cut(s) 135, 343, 378, 406
Hpy188I TCNGA 2 cut(s) 330, 648
Hpy188III TCNNGA 5 cut(s) 448, 488, 595, 637, 791
Hpy8I GTNNAC 4 cut(s) 135, 343, 378, 406
Hpy99I CGWCG 2 cut(s) 347, 518
HpyAV CCTTC 4 cut(s) 355, 469, 539, 747
HpyCH4III ACNGT 7 cut(s) 25, 109, 143, 353, 427, 601, 703
HpyCH4V TGCA 6 cut(s) 127, 260, 466, 606, 615, 773
HpyF10VI GCNNNNNNNGC 6 cut(s) 97, 170, 257, 612, 689, 794
HpyF3I CTNAG 7 cut(s) 78, 160, 193, 206, 329, 534, 789
Hsp92I GRCGYC 1 cut(s) 516
KflI GGGWCCC 1 cut(s) 64
KspAI GTTAAC 1 cut(s) 406
Kzo9I GATC 2 cut(s) 580, 639
LmnI GCTCC 1 cut(s) 802
Lsp1109I GCAGC 2 cut(s) 513, 517
LweI GCATC 2 cut(s) 103, 784
MaeI CTAG 2 cut(s) 335, 585
MaeIII GTNAC 4 cut(s) 25, 143, 353, 421
MalI GATC 2 cut(s) 582, 641
MboI GATC 2 cut(s) 580, 639
MboII GAAGA 2 cut(s) 767, 770
MfeI CAATTG 1 cut(s) 670
MflI RGATCY 2 cut(s) 580, 639
MlsI TGGCCA 1 cut(s) 560
MluCI AATT 2 cut(s) 17, 670
MluNI TGGCCA 1 cut(s) 560
MlyI GAGTC 2 cut(s) 383, 397
Mox20I TGGCCA 1 cut(s) 560
MscI TGGCCA 1 cut(s) 560
MseI TTAA 3 cut(s) 384, 405, 510
Msp20I TGGCCA 1 cut(s) 560
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 4 cut(s) 68, 470, 562, 570
MunI CAATTG 1 cut(s) 670
Mva1269I GAATGC 1 cut(s) 606
MvaI CCWGG 3 cut(s) 470, 562, 570
MwoI GCNNNNNNNGC 6 cut(s) 97, 170, 257, 612, 689, 794
NciI CCSGG 1 cut(s) 68
NcoI CCATGG 1 cut(s) 725
NdeII GATC 2 cut(s) 580, 639
NlaIV GGNNCC 4 cut(s) 65, 66, 567, 574
NmuCI GTSAC 2 cut(s) 143, 421
NspI RCATGY 3 cut(s) 58, 422, 526
PceI AGGCCT 1 cut(s) 497
PctI GAATGC 1 cut(s) 606
PfeI GAWTC 2 cut(s) 325, 649
PkrI GCNGC 2 cut(s) 507, 528
PleI GAGTC 2 cut(s) 382, 396
PpsI GAGTC 2 cut(s) 382, 396
PpuMI RGGWCCY 1 cut(s) 64
PshAI GACNNNNGTC 1 cut(s) 428
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 3 cut(s) 468, 560, 568
PspGI CCWGG 3 cut(s) 468, 560, 568
PspN4I GGNNCC 4 cut(s) 65, 66, 567, 574
PspPI GGNCC 2 cut(s) 64, 573
PspPPI RGGWCCY 1 cut(s) 64
PsuI RGATCY 2 cut(s) 580, 639
RsaI GTAC 2 cut(s) 350, 371
RsaNI GTAC 2 cut(s) 349, 370
SalI GTCGAC 1 cut(s) 341
SaqAI TTAA 3 cut(s) 384, 405, 510
SatI GCNGC 2 cut(s) 506, 527
Sau3AI GATC 2 cut(s) 580, 639
Sau96I GGNCC 2 cut(s) 64, 573
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 2 cut(s) 383, 397
ScrFI CCNGG 4 cut(s) 68, 470, 562, 570
SfaNI GCATC 2 cut(s) 103, 784
SfcI CTRYAG 1 cut(s) 693
SinI GGWCC 1 cut(s) 64
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 2 cut(s) 17, 670
SseBI AGGCCT 1 cut(s) 497
SspI AATATT 1 cut(s) 35
SspMI CTAG 2 cut(s) 335, 585
StuI AGGCCT 1 cut(s) 497
StyD4I CCNGG 4 cut(s) 66, 468, 560, 568
StyI CCWWGG 1 cut(s) 725
TaaI ACNGT 7 cut(s) 25, 109, 143, 353, 427, 601, 703
TaqI TCGA 4 cut(s) 59, 227, 342, 676
TasI AATT 2 cut(s) 17, 670
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 2 cut(s) 325, 649
Tru1I TTAA 3 cut(s) 384, 405, 510
Tru9I TTAA 3 cut(s) 384, 405, 510
TscAI CASTG 4 cut(s) 112, 148, 617, 668
TseFI GTSAC 2 cut(s) 143, 421
TseI GCWGC 2 cut(s) 505, 526
Tsp45I GTSAC 2 cut(s) 143, 421
TspDTI ATGAA 4 cut(s) 255, 374, 696, 729
TspGWI ACGGA 1 cut(s) 789
TspRI CASTG 4 cut(s) 112, 148, 617, 668
VpaK11BI GGWCC 1 cut(s) 64
XceI RCATGY 3 cut(s) 58, 422, 526
XmiI GTMKAC 3 cut(s) 134, 342, 377
XspI CTAG 2 cut(s) 335, 585
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.