Rorug06G0160100

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002266
Physical Location & Seq
Forward (+)
22811410 .. 22817217
5808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0160100.1

Sequence Viewer

Length: 672 bp
ATGTATCATCCTACTAGAGGTGGAGTTCGTGGTGGTCGAGATCAGTTTAGTTGGGACGATGTTAAGGTCGACAAGCATCGTGAGAATTATCTTGGTCACAGTATCAAGGCCCCTGTTGGTAGATGGCAAAAAGGTAAGGATCTTCACTGGTATAACAAAGACAAAAAAACATGGAGTGCCGAAATGGATGCTGCAAAAGATGAGATTAAAAGAATCAAGGAGGAAGAGGATCAGGCTATGAGGGAGGCTCTTGGCTTAGCTCCTAAGCGTGCAAACCGAGCTCAAGGTAATCGGCTTGATAAGCATGAGTTCTCAGAACTTGTGAAGCGAGGTTCTACGGCAGAGGACTTGGGTGAAGGGCATGCTGAAGCAGCACACGTACATGGTCTTGGTTTTTCAAGAGCACCTGTCCCTTGGGATGATTCAACTTCTCTTCAACCTACAGTCAAGGGGGGTGCACCTGAAATGGGGGATATTCCTGTGCCTAAGCTTCCACCAAAAGAAAAAGTTGAAGAGGAGTCCGAGGATGAAAGCAGTCGAAGGAAAAGGAGGCGTGAAGAGAGGAAGCATGAGAAACATGAGAGGCGTGAGAAACGCGATAAGCATGAAAAACGGCATTCCTCAGATGAGAGAAAACATAGGAAAGACAAGGAGAAGAGGAGACATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

223

Amino Acids

26.02

Weight (kDa)

9.54

Isoelectric Point (pI)

61.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMtag PF10159 8 - 85 9.9e-35 Multiple myeloma tumor-associated
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 69
AccII CGCG 1 cut(s) 597
AclWI GGATC 2 cut(s) 147, 237
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 1 cut(s) 381
AfiI CCNNNNNNNGG 2 cut(s) 17, 467
AflIII ACRYGT 1 cut(s) 376
AgsI TTSAA 4 cut(s) 399, 426, 437, 512
AluBI AGCT 3 cut(s) 260, 281, 490
AluI AGCT 3 cut(s) 260, 281, 490
Alw21I GWGCWC 3 cut(s) 283, 406, 460
Alw26I GTCTC 1 cut(s) 655
Alw44I GTGCAC 1 cut(s) 456
AlwI GGATC 2 cut(s) 147, 237
AoxI GGCC 1 cut(s) 108
ApaLI GTGCAC 1 cut(s) 456
ApeKI GCWGC 2 cut(s) 191, 371
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 460
BanII GRGCYC 1 cut(s) 283
Bbv12I GWGCWC 3 cut(s) 283, 406, 460
BbvI GCAGC 2 cut(s) 178, 383
BccI CCATC 1 cut(s) 117
BceAI ACGGC 2 cut(s) 354, 629
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BcoDI GTCTC 1 cut(s) 655
BfaI CTAG 1 cut(s) 15
BfmI CTRYAG 1 cut(s) 441
BisI GCNGC 2 cut(s) 192, 372
BlpI GCTNAGC 1 cut(s) 256
BlsI GCNGC 2 cut(s) 193, 373
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 111
BmsI GCATC 2 cut(s) 85, 178
BplI GAGNNNNNCTC 2 cut(s) 232, 264
Bpu10I CCTNAGC 2 cut(s) 264, 486
Bpu1102I GCTNAGC 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 267
BsaAI YACGTR 1 cut(s) 379
BsaJI CCNNGG 2 cut(s) 413, 522
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 467
Bse1I ACTGG 1 cut(s) 152
BseDI CCNNGG 2 cut(s) 413, 522
BseGI GGATG 4 cut(s) 7, 193, 424, 532
BseLI CCNNNNNNNGG 2 cut(s) 17, 467
BseMII CTCAG 2 cut(s) 327, 636
BseNI ACTGG 1 cut(s) 152
BseRI GAGGAG 1 cut(s) 530
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 2 cut(s) 178, 383
Bsh1236I CGCG 1 cut(s) 597
BshFI GGCC 1 cut(s) 110
BsiHKAI GWGCWC 3 cut(s) 283, 406, 460
BslFI GGGAC 2 cut(s) 68, 395
BslI CCNNNNNNNGG 2 cut(s) 17, 467
BsmAI GTCTC 1 cut(s) 655
BsmFI GGGAC 2 cut(s) 68, 395
BsmI GAATGC 1 cut(s) 616
BsnI GGCC 1 cut(s) 110
Bsp1286I GDGCHC 3 cut(s) 283, 406, 460
Bsp143I GATC 3 cut(s) 40, 139, 229
Bsp1720I GCTNAGC 1 cut(s) 256
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 2 cut(s) 326, 635
BspFNI CGCG 1 cut(s) 597
BspLI GGNNCC 1 cut(s) 111
BspPI GGATC 2 cut(s) 147, 237
BsrI ACTGG 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 413, 522
BssMI GATC 3 cut(s) 40, 139, 229
BssT1I CCWWGG 1 cut(s) 413
Bst4CI ACNGT 2 cut(s) 101, 445
Bst6I CTCTTC 5 cut(s) 219, 438, 507, 552, 650
BstBAI YACGTR 1 cut(s) 379
BstC8I GCNNGC 2 cut(s) 270, 363
BstDEI CTNAG 6 cut(s) 256, 264, 313, 486, 622, 669
BstENI CCTNNNNNAGG 1 cut(s) 15
BstF5I GGATG 4 cut(s) 7, 193, 424, 532
BstFNI CGCG 1 cut(s) 597
BstKTI GATC 3 cut(s) 43, 142, 232
BstMAI GTCTC 1 cut(s) 655
BstMBI GATC 3 cut(s) 40, 139, 229
BstMWI GCNNNNNNNGC 3 cut(s) 278, 301, 371
BstNSI RCATGY 2 cut(s) 365, 668
BstSFI CTRYAG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 460
BstUI CGCG 1 cut(s) 597
BstV1I GCAGC 2 cut(s) 178, 383
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 1 cut(s) 110
BtsCI GGATG 4 cut(s) 7, 193, 424, 532
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 2 cut(s) 270, 363
Cfr13I GGNCC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 380
CviAII CATG 8 cut(s) 171, 305, 362, 383, 569, 578, 605, 665
CviJI RGCY 8 cut(s) 110, 236, 248, 255, 260, 281, 295, 490
CviKI_1 RGCY 8 cut(s) 110, 236, 248, 255, 260, 281, 295, 490
CviQI GTAC 1 cut(s) 380
DdeI CTNAG 6 cut(s) 256, 264, 313, 486, 622, 669
DpnI GATC 3 cut(s) 42, 141, 231
DpnII GATC 3 cut(s) 40, 139, 229
Eam1104I CTCTTC 5 cut(s) 219, 438, 507, 552, 650
EarI CTCTTC 5 cut(s) 219, 438, 507, 552, 650
Ecl136II GAGCTC 1 cut(s) 281
Eco130I CCWWGG 1 cut(s) 413
Eco24I GRGCYC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 387
EcoICRI GAGCTC 1 cut(s) 281
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 413
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 413
FaeI CATG 8 cut(s) 174, 308, 365, 386, 572, 581, 608, 668
FaqI GGGAC 2 cut(s) 68, 395
FatI CATG 8 cut(s) 170, 304, 361, 382, 568, 577, 604, 664
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 2 cut(s) 192, 372
FokI GGATG 3 cut(s) 200, 431, 539
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 2 cut(s) 192, 372
FspBI CTAG 1 cut(s) 15
GluI GCNGC 2 cut(s) 192, 372
HaeIII GGCC 1 cut(s) 110
Hin1II CATG 8 cut(s) 174, 308, 365, 386, 572, 581, 608, 668
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 3 cut(s) 213, 422, 518
HphI GGTGA 1 cut(s) 365
Hpy166II GTNNAC 2 cut(s) 70, 458
Hpy188I TCNGA 3 cut(s) 316, 523, 625
Hpy188III TCNNGA 3 cut(s) 38, 80, 399
Hpy8I GTNNAC 2 cut(s) 70, 458
HpyAV CCTTC 2 cut(s) 350, 534
HpyCH4III ACNGT 2 cut(s) 101, 445
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 3 cut(s) 194, 272, 458
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 301, 371
HpyF3I CTNAG 6 cut(s) 256, 264, 313, 486, 622, 669
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 8 cut(s) 174, 308, 365, 386, 572, 581, 608, 668
Kzo9I GATC 3 cut(s) 40, 139, 229
LmnI GCTCC 1 cut(s) 265
LpnPI CCDG 6 cut(s) 126, 133, 218, 420, 474, 492
Lsp1109I GCAGC 2 cut(s) 178, 383
LweI GCATC 2 cut(s) 85, 178
MaeI CTAG 1 cut(s) 15
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 95
MalI GATC 3 cut(s) 42, 141, 231
MboI GATC 3 cut(s) 40, 139, 229
MboII GAAGA 6 cut(s) 134, 236, 425, 524, 569, 667
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 3 cut(s) 283, 406, 460
MluCI AATT 1 cut(s) 85
MlyI GAGTC 1 cut(s) 527
MseI TTAA 2 cut(s) 63, 207
MslI CAYNNNNRTG 1 cut(s) 381
Mva1269I GAATGC 1 cut(s) 616
MvnI CGCG 1 cut(s) 597
MwoI GCNNNNNNNGC 3 cut(s) 278, 301, 371
NdeII GATC 3 cut(s) 40, 139, 229
NlaIII CATG 8 cut(s) 174, 308, 365, 386, 572, 581, 608, 668
NlaIV GGNNCC 1 cut(s) 111
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 2 cut(s) 365, 668
PaeI GCATGC 1 cut(s) 365
PcsI WCGNNNNNNNCGW 1 cut(s) 34
PctI GAATGC 1 cut(s) 616
PfeI GAWTC 2 cut(s) 213, 422
PkrI GCNGC 2 cut(s) 193, 373
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Ppu21I YACGTR 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 283
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 1 cut(s) 109
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 1 cut(s) 381
RsaNI GTAC 1 cut(s) 380
RseI CAYNNNNRTG 1 cut(s) 381
SacI GAGCTC 1 cut(s) 283
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 2 cut(s) 63, 207
SatI GCNGC 2 cut(s) 192, 372
Sau3AI GATC 3 cut(s) 40, 139, 229
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 1 cut(s) 527
SduI GDGCHC 3 cut(s) 283, 406, 460
SfaNI GCATC 2 cut(s) 85, 178
SfcI CTRYAG 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
SphI GCATGC 1 cut(s) 365
Sse9I AATT 1 cut(s) 85
SspMI CTAG 1 cut(s) 15
SstI GAGCTC 1 cut(s) 283
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 2 cut(s) 101, 445
TaiI ACGT 1 cut(s) 381
TaqI TCGA 3 cut(s) 37, 69, 538
TasI AATT 1 cut(s) 85
TfiI GAWTC 2 cut(s) 213, 422
Tru1I TTAA 2 cut(s) 63, 207
Tru9I TTAA 2 cut(s) 63, 207
TscAI CASTG 1 cut(s) 152
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 2 cut(s) 191, 371
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 2 cut(s) 543, 621
TspRI CASTG 1 cut(s) 152
VneI GTGCAC 1 cut(s) 456
XagI CCTNNNNNAGG 1 cut(s) 15
XceI RCATGY 2 cut(s) 365, 668
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.