FvH4_2g24380

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001757
Physical Location & Seq
Forward (+)
19921298 .. 19923166
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24380.t1

Sequence Viewer

Length: 1044 bp
ATGGAGTCATCGGTGCAGTTCTACAACACAATCTCAAAGGAAGAAGACTTATATGTGATGAACAAAACGAAAACCAACACTACTATGCAAAGGCAAAATAGGGCATGGTCTGATCTCCCTCCTGAGCTTCTTTCACTCATAGCAGAGCGTGCAGGCCTATTCGAGCTTCTGAGTTTCTTTCGTGTTTGCAACACTTGGCATTCTGCTTCTTCCACCGCCTTAGCTCACATTGCTGAGCATGAAGTAAACAAAGGGCCTTGGTTTCTACTATACGAGGATAAAAACTCCGAGTGTCAGTTGCTAACTGCAACTGGTAAAAAGTTCACCACCAGTCTCCCTGAACTGGATGGAACAACTTGCCTTGCAACGTACGGGGCATGGTTTCTGTTATTCAAACAAGGAGGTTCCATGTTCTTCTTTCACGTCTTGTCGCGCCAGAGAATAGACCTTCCTGAATTCCCAGTTCCAGAACTCACTGATCATGTTGGAGCAGTTTCATCTTCTCCAACTTCCCAAGATTGTGTGGTTTGTGTCATTAGCCGTAGCGATGATGAGACATTGGATGCCAAACTACTTTTCCTTAGAGACAAGACTTGGATTGGATCAGAGTATTCTCATCCTCGAGATAAAACAGACACAATCAAATCTGCAGCCTATTACAATGGAACCTTCGGCTTTTTTGATGGTAGAAGTCAAAATATGAGCCGGATGACCATATCTATTGATGAAAACCATTCTCCCCACTGGGAATATAAGAAACTCCTAGATGTAACCAATGAGGATCGTCACAAATACTCAAAATCATCTACGTTTCGTCGTGACGTGAATGAGTTGAAGAATAATTGGAAGGACATGAAGAAGAAGCTGGGGCTGACAGAGGATGTTTCAGTTTCTACTTGTGGGACAATTACACCCTTAATAGAAGAAGATTTGAGCAAGTTTATTTTTAATGAGAGCGTCAGCGAGAACGATGACAAATCAAAGAACCGTTGTTTCAAGGGGGTATGGATCCAACCAAGATCCTCAGGAGATGATGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

348

Amino Acids

39.7

Weight (kDa)

5.56

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 106 - 244 4.8e-14 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 433
AciI CCGC 1 cut(s) 216
AclWI GGATC 5 cut(s) 610, 789, 1003, 1014, 1016
AcsI RAATTY 1 cut(s) 455
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 1 cut(s) 343
AgsI TTSAA 3 cut(s) 394, 835, 997
AjiI CACGTC 2 cut(s) 424, 823
AjuI GAANNNNNNNTTGG 2 cut(s) 560, 592
AluBI AGCT 4 cut(s) 127, 166, 224, 865
AluI AGCT 4 cut(s) 127, 166, 224, 865
Alw26I GTCTC 3 cut(s) 338, 548, 579
AlwI GGATC 5 cut(s) 610, 789, 1003, 1014, 1016
Ama87I CYCGRG 1 cut(s) 621
AoxI GGCC 2 cut(s) 154, 254
ApeKI GCWGC 1 cut(s) 650
ApoI RAATTY 1 cut(s) 455
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 1 cut(s) 254
AsuHPI GGTGA 1 cut(s) 316
AvaI CYCGRG 1 cut(s) 621
AxyI CCTNAGG 1 cut(s) 1024
BamHI GGATCC 1 cut(s) 1008
BbsI GAAGAC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 662
BccI CCATC 2 cut(s) 341, 677
BceAI ACGGC 1 cut(s) 525
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 3 cut(s) 338, 548, 579
BfaI CTAG 1 cut(s) 764
BfmI CTRYAG 1 cut(s) 648
BisI GCNGC 1 cut(s) 651
BlpI GCTNAGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 652
BmeT110I CYCGRG 1 cut(s) 621
BmgBI CACGTC 2 cut(s) 424, 823
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 3 cut(s) 406, 667, 1010
BmrI ACTGGG 2 cut(s) 455, 754
BmsI GCATC 1 cut(s) 553
BmuI ACTGGG 2 cut(s) 455, 754
BpiI GAAGAC 1 cut(s) 51
Bpu10I CCTNAGC 2 cut(s) 123, 220
Bpu1102I GCTNAGC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 257
Bsc4I CCNNNNNNNGG 1 cut(s) 343
Bse1I ACTGG 5 cut(s) 316, 330, 348, 461, 749
Bse21I CCTNAGG 1 cut(s) 1024
Bse3DI GCAATG 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 257
BseGI GGATG 5 cut(s) 352, 568, 616, 714, 886
BseLI CCNNNNNNNGG 1 cut(s) 343
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 4 cut(s) 114, 161, 225, 1038
BseNI ACTGG 5 cut(s) 316, 330, 348, 461, 749
BseXI GCAGC 1 cut(s) 662
BseYI CCCAGC 1 cut(s) 865
BsgI GTGCAG 2 cut(s) 35, 171
Bsh1236I CGCG 1 cut(s) 433
BshFI GGCC 2 cut(s) 156, 256
BsiHKCI CYCGRG 1 cut(s) 621
BsiSI CCGG 1 cut(s) 706
BsiWI CGTACG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 916
BslI CCNNNNNNNGG 1 cut(s) 343
BsmAI GTCTC 3 cut(s) 338, 548, 579
BsmFI GGGAC 1 cut(s) 916
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 2 cut(s) 156, 256
BsoBI CYCGRG 1 cut(s) 621
Bsp143I GATC 6 cut(s) 112, 478, 602, 781, 1008, 1019
Bsp1720I GCTNAGC 1 cut(s) 234
BspACI CCGC 1 cut(s) 216
BspANI GGCC 2 cut(s) 156, 256
BspCNI CTCAG 4 cut(s) 115, 162, 226, 1037
BspFNI CGCG 1 cut(s) 433
BspLI GGNNCC 3 cut(s) 406, 667, 1010
BspMAI CTGCAG 1 cut(s) 652
BspPI GGATC 5 cut(s) 610, 789, 1003, 1014, 1016
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 5 cut(s) 316, 330, 348, 461, 749
BssECI CCNNGG 1 cut(s) 257
BssMI GATC 6 cut(s) 112, 478, 602, 781, 1008, 1019
BssT1I CCWWGG 1 cut(s) 257
Bst4CI ACNGT 1 cut(s) 989
BstAPI GCANNNNNTGC 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 150, 154
BstDEI CTNAG 6 cut(s) 123, 170, 220, 234, 581, 1024
BstF5I GGATG 5 cut(s) 352, 568, 616, 714, 886
BstFNI CGCG 1 cut(s) 433
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 6 cut(s) 115, 481, 605, 784, 1011, 1022
BstMAI GTCTC 3 cut(s) 338, 548, 579
BstMBI GATC 6 cut(s) 112, 478, 602, 781, 1008, 1019
BstMWI GCNNNNNNNGC 2 cut(s) 149, 230
BstSFI CTRYAG 1 cut(s) 648
BstUI CGCG 1 cut(s) 433
BstV1I GCAGC 1 cut(s) 662
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 2 cut(s) 1008, 1019
BstYI RGATCY 2 cut(s) 1008, 1019
Bsu36I CCTNAGG 1 cut(s) 1024
BsuRI GGCC 2 cut(s) 156, 256
BtgZI GCGATG 1 cut(s) 561
BtrI CACGTC 2 cut(s) 424, 823
BtsCI GGATG 5 cut(s) 352, 568, 616, 714, 886
BtsIMutI CAGTG 2 cut(s) 474, 742
Cac8I GCNNGC 2 cut(s) 150, 154
CfoI GCGC 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 254
CseI GACGC 1 cut(s) 946
Csp6I GTAC 1 cut(s) 370
CviAII CATG 6 cut(s) 105, 239, 378, 409, 482, 853
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 6 cut(s) 123, 170, 220, 234, 581, 1024
DpnI GATC 6 cut(s) 114, 480, 604, 783, 1010, 1021
DpnII GATC 6 cut(s) 112, 478, 602, 781, 1008, 1019
Eco130I CCWWGG 1 cut(s) 257
Eco147I AGGCCT 1 cut(s) 156
Eco81I CCTNAGG 1 cut(s) 1024
Eco88I CYCGRG 1 cut(s) 621
EcoO109I RGGNCCY 1 cut(s) 254
EcoRI GAATTC 1 cut(s) 455
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
FaeI CATG 6 cut(s) 108, 242, 381, 412, 485, 856
FaqI GGGAC 1 cut(s) 916
FatI CATG 6 cut(s) 104, 238, 377, 408, 481, 852
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 1 cut(s) 651
FokI GGATG 5 cut(s) 359, 575, 603, 721, 893
Fsp4HI GCNGC 1 cut(s) 651
FspBI CTAG 1 cut(s) 764
GlaI GCGC 1 cut(s) 434
GluI GCNGC 1 cut(s) 651
GsaI CCCAGC 1 cut(s) 869
HaeIII GGCC 2 cut(s) 156, 256
HapII CCGG 1 cut(s) 706
HgaI GACGC 1 cut(s) 946
HhaI GCGC 1 cut(s) 435
Hin1II CATG 6 cut(s) 108, 242, 381, 412, 485, 856
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 706
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 2 cut(s) 247, 324
Hpy188I TCNGA 4 cut(s) 112, 171, 289, 607
Hpy188III TCNNGA 6 cut(s) 122, 452, 467, 623, 818, 1026
Hpy8I GTNNAC 2 cut(s) 247, 324
Hpy99I CGWCG 1 cut(s) 819
HpyAV CCTTC 3 cut(s) 458, 679, 841
HpyCH4III ACNGT 1 cut(s) 989
HpyCH4IV ACGT 4 cut(s) 368, 423, 809, 822
HpyCH4V TGCA 7 cut(s) 16, 88, 152, 189, 308, 365, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 230
HpyF3I CTNAG 6 cut(s) 123, 170, 220, 234, 581, 1024
HpySE526I ACGT 4 cut(s) 368, 423, 809, 822
Hsp92II CATG 6 cut(s) 108, 242, 381, 412, 485, 856
HspAI GCGC 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 6 cut(s) 112, 478, 602, 781, 1008, 1019
LmnI GCTCC 1 cut(s) 488
Lsp1109I GCAGC 1 cut(s) 662
LweI GCATC 1 cut(s) 553
MaeI CTAG 1 cut(s) 764
MaeII ACGT 4 cut(s) 368, 423, 809, 822
MaeIII GTNAC 3 cut(s) 769, 785, 818
MalI GATC 6 cut(s) 114, 480, 604, 783, 1010, 1021
MboI GATC 6 cut(s) 112, 478, 602, 781, 1008, 1019
MflI RGATCY 2 cut(s) 1008, 1019
MluCI AATT 3 cut(s) 455, 841, 906
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 3 cut(s) 466, 530, 1036
MnlI CCTC 7 cut(s) 129, 268, 395, 630, 772, 871, 1033
MseI TTAA 2 cut(s) 917, 948
MslI CAYNNNNRTG 1 cut(s) 83
MspI CCGG 1 cut(s) 706
Mva1269I GAATGC 1 cut(s) 199
MvnI CGCG 1 cut(s) 433
MwoI GCNNNNNNNGC 2 cut(s) 149, 230
NdeII GATC 6 cut(s) 112, 478, 602, 781, 1008, 1019
NlaIII CATG 6 cut(s) 108, 242, 381, 412, 485, 856
NlaIV GGNNCC 3 cut(s) 406, 667, 1010
NmuCI GTSAC 2 cut(s) 785, 818
PaeR7I CTCGAG 1 cut(s) 621
PceI AGGCCT 1 cut(s) 156
PctI GAATGC 1 cut(s) 199
Pfl23II CGTACG 1 cut(s) 369
PkrI GCNGC 1 cut(s) 652
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 865
PspLI CGTACG 1 cut(s) 369
PspN4I GGNNCC 3 cut(s) 406, 667, 1010
PspPI GGNCC 1 cut(s) 254
PstI CTGCAG 1 cut(s) 652
PsuI RGATCY 2 cut(s) 1008, 1019
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 83
SaqAI TTAA 2 cut(s) 917, 948
SatI GCNGC 1 cut(s) 651
Sau3AI GATC 6 cut(s) 112, 478, 602, 781, 1008, 1019
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 1 cut(s) 14
SfaNI GCATC 1 cut(s) 553
SfcI CTRYAG 1 cut(s) 648
Sfr274I CTCGAG 1 cut(s) 621
SlaI CTCGAG 1 cut(s) 621
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
Sse9I AATT 3 cut(s) 455, 841, 906
SseBI AGGCCT 1 cut(s) 156
SsiI CCGC 1 cut(s) 216
SspMI CTAG 1 cut(s) 764
StuI AGGCCT 1 cut(s) 156
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 1 cut(s) 989
TaiI ACGT 4 cut(s) 371, 426, 812, 825
TaqI TCGA 2 cut(s) 162, 622
TasI AATT 3 cut(s) 455, 841, 906
Tru1I TTAA 2 cut(s) 917, 948
Tru9I TTAA 2 cut(s) 917, 948
TscAI CASTG 2 cut(s) 481, 749
TseFI GTSAC 2 cut(s) 785, 818
TseI GCWGC 1 cut(s) 650
Tsp45I GTSAC 2 cut(s) 785, 818
TspDTI ATGAA 5 cut(s) 74, 255, 486, 741, 869
TspRI CASTG 2 cut(s) 481, 749
XapI RAATTY 1 cut(s) 455
XhoI CTCGAG 1 cut(s) 621
XspI CTAG 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.