Prupe.4G274600_v2.0.a1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001757
Physical Location & Seq
Reverse (-)
22888337 .. 22889898
1562 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G274600.1

Sequence Viewer

Length: 792 bp
ATGAGGAAAAAAGTGTGCATGGAAAGCCGTCCCCCGCCGCCCTTGTCCGATCTTCTTCCTCCTGAACTGTTGTCAAAAATAGCAGATGGTCTAAGCCTAATCGATCTTCTGAGTTTCCGCAGTGTCTGCAAGGCTTGGAACTCTGCTTCTTCAACAGCTTCAGCTCAGAATGAATCTTCATATGAACCCTGGTTTTTACTGTACGAGGAACATTCTGATCAATGCCAGTTGCTAATATGTGAAAGTGCCTCACAACTATGTGAGCATGTTGCAGCATTTTCATCCCCTCCCACTTGTCAAGATTGCATTGTTTTTGTCATTAGCCGTATCAATGATTTAAAACTGGAGCTGAATGTTCTTCATCGCGGAGCCAATGAATGGATCCGACACAAGCGCAATTTCACCCCATGTCCAGTTAACAGAATTGAATGTGGTGCTTTTCACAATGGAGAATTCTATTTTTTCGCCTGCGGAGGAGACAGGTTGATCCAGGTCGTTGTCACTGCTGCCGGCAAGCTACAGTGCCAGTGCTTTTCCATTATTCCTGTTTCGGCACCGGCACCGGCACCAGCACCAGCACCAGCACCAGCATCAGCACCAGCACCAGCTGAAGATGAAATTGTATCATTGAGAGAGGAAATGGGTTATTTGGACAAAAAGAATGTGAAGCAGCAGCTGGGGATGGGAAGGGACGTTTCAATTTCCTCATCCAAGACCAAGAGCCCTGATTTTAAAGGGATTTGGATCCACCCAAGATTCTGTCAAATCTCCCCAATTCACCAGAGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

264

Amino Acids

29.07

Weight (kDa)

6.38

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 553, 559, 565
AccB7I CCANNNNNTGG 1 cut(s) 378
AccII CGCG 1 cut(s) 366
AciI CCGC 5 cut(s) 35, 38, 118, 366, 471
AclWI GGATC 5 cut(s) 376, 389, 481, 739, 752
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 2 cut(s) 144, 630
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 378
AgsI TTSAA 3 cut(s) 153, 428, 699
AjnI CCWGG 2 cut(s) 188, 489
AluBI AGCT 6 cut(s) 158, 164, 349, 517, 608, 676
AluI AGCT 6 cut(s) 158, 164, 349, 517, 608, 676
Alw26I GTCTC 1 cut(s) 471
AlwI GGATC 5 cut(s) 376, 389, 481, 739, 752
AlwNI CAGNNNCTG 2 cut(s) 126, 676
ApeKI GCWGC 4 cut(s) 272, 506, 670, 673
ApoI RAATTY 1 cut(s) 452
AspLEI GCGC 1 cut(s) 396
AsuHPI GGTGA 2 cut(s) 394, 770
BamHI GGATCC 2 cut(s) 381, 744
BanI GGYRCC 3 cut(s) 553, 559, 565
BanII GRGCYC 1 cut(s) 725
BbvI GCAGC 4 cut(s) 284, 493, 682, 685
BccI CCATC 2 cut(s) 80, 676
BceAI ACGGC 2 cut(s) 12, 309
BciT130I CCWGG 2 cut(s) 190, 491
BclI TGATCA 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 518
BisI GCNGC 5 cut(s) 38, 273, 507, 671, 674
BlsI GCNGC 5 cut(s) 39, 274, 508, 672, 675
Bme1390I CCNGG 2 cut(s) 190, 491
BmiI GGNNCC 6 cut(s) 370, 383, 555, 561, 567, 746
BmrFI CCNGG 2 cut(s) 190, 491
BmsI GCATC 1 cut(s) 599
BpmI CTGGAG 1 cut(s) 365
Bsa29I ATCGAT 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 743
BsaJI CCNNGG 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse118I RCCGGY 3 cut(s) 509, 556, 562
Bse1I ACTGG 4 cut(s) 226, 348, 413, 526
Bse8I GATNNNNATC 1 cut(s) 743
BseBI CCWGG 2 cut(s) 190, 491
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 188
BseGI GGATG 3 cut(s) 281, 687, 707
BseJI GATNNNNATC 1 cut(s) 743
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMII CTCAG 2 cut(s) 101, 179
BseNI ACTGG 4 cut(s) 226, 348, 413, 526
BseRI GAGGAG 1 cut(s) 489
BseXI GCAGC 4 cut(s) 284, 493, 682, 685
BseYI CCCAGC 1 cut(s) 676
Bsh1236I CGCG 1 cut(s) 366
BshNI GGYRCC 3 cut(s) 553, 559, 565
BshVI ATCGAT 1 cut(s) 102
BsiSI CCGG 3 cut(s) 510, 557, 563
BslFI GGGAC 2 cut(s) 15, 704
BslI CCNNNNNNNGG 1 cut(s) 378
BsmAI GTCTC 1 cut(s) 471
BsmFI GGGAC 2 cut(s) 15, 704
Bsp1286I GDGCHC 1 cut(s) 725
Bsp143I GATC 6 cut(s) 49, 103, 217, 381, 486, 744
BspACI CCGC 5 cut(s) 35, 38, 118, 366, 471
BspCNI CTCAG 2 cut(s) 102, 178
BspDI ATCGAT 1 cut(s) 102
BspFNI CGCG 1 cut(s) 366
BspLI GGNNCC 6 cut(s) 370, 383, 555, 561, 567, 746
BspPI GGATC 5 cut(s) 376, 389, 481, 739, 752
BspT107I GGYRCC 3 cut(s) 553, 559, 565
BsrFI RCCGGY 3 cut(s) 509, 556, 562
BsrI ACTGG 4 cut(s) 226, 348, 413, 526
BssAI RCCGGY 3 cut(s) 509, 556, 562
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 6 cut(s) 49, 103, 217, 381, 486, 744
Bst2UI CCWGG 2 cut(s) 190, 491
Bst4CI ACNGT 3 cut(s) 69, 201, 522
BstAPI GCANNNNNTGC 1 cut(s) 126
BstC8I GCNNGC 3 cut(s) 469, 511, 515
BstDEI CTNAG 3 cut(s) 92, 110, 165
BstF5I GGATG 3 cut(s) 281, 687, 707
BstFNI CGCG 1 cut(s) 366
BstHHI GCGC 1 cut(s) 396
BstKTI GATC 6 cut(s) 52, 106, 220, 384, 489, 747
BstMAI GTCTC 1 cut(s) 471
BstMBI GATC 6 cut(s) 49, 103, 217, 381, 486, 744
BstMWI GCNNNNNNNGC 2 cut(s) 24, 126
BstNI CCWGG 2 cut(s) 190, 491
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 188, 489
BstSFI CTRYAG 1 cut(s) 518
BstUI CGCG 1 cut(s) 366
BstV1I GCAGC 4 cut(s) 284, 493, 682, 685
BstX2I RGATCY 2 cut(s) 381, 744
BstYI RGATCY 2 cut(s) 381, 744
Bsu15I ATCGAT 1 cut(s) 102
BsuTUI ATCGAT 1 cut(s) 102
BtgZI GCGATG 1 cut(s) 347
BtsCI GGATG 3 cut(s) 281, 687, 707
BtsI GCAGTG 2 cut(s) 127, 501
BtsIMutI CAGTG 4 cut(s) 127, 501, 527, 533
Cac8I GCNNGC 3 cut(s) 469, 511, 515
CaiI CAGNNNCTG 2 cut(s) 126, 676
CfoI GCGC 1 cut(s) 396
Cfr10I RCCGGY 3 cut(s) 509, 556, 562
ClaI ATCGAT 1 cut(s) 102
Csp6I GTAC 1 cut(s) 202
CviAII CATG 3 cut(s) 19, 266, 408
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 3 cut(s) 92, 110, 165
DpnI GATC 6 cut(s) 51, 105, 219, 383, 488, 746
DpnII GATC 6 cut(s) 49, 103, 217, 381, 486, 744
DraI TTTAAA 2 cut(s) 339, 733
Eco24I GRGCYC 1 cut(s) 725
Eco57I CTGAAG 2 cut(s) 144, 630
EcoRI GAATTC 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 188, 489
EcoT38I GRGCYC 1 cut(s) 725
FaeI CATG 3 cut(s) 22, 269, 411
FaiI YATR 7 cut(s) 20, 181, 183, 238, 259, 267, 409
FaqI GGGAC 2 cut(s) 15, 704
FatI CATG 3 cut(s) 18, 265, 407
FauI CCCGC 1 cut(s) 42
FauNDI CATATG 1 cut(s) 181
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 5 cut(s) 38, 273, 507, 671, 674
FokI GGATG 3 cut(s) 268, 694, 694
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 5 cut(s) 38, 273, 507, 671, 674
GlaI GCGC 1 cut(s) 395
GluI GCNGC 5 cut(s) 38, 273, 507, 671, 674
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 1 cut(s) 365
HapII CCGG 3 cut(s) 510, 557, 563
HhaI GCGC 1 cut(s) 396
Hin1II CATG 3 cut(s) 22, 269, 411
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 2 cut(s) 173, 756
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 3 cut(s) 510, 557, 563
HphI GGTGA 2 cut(s) 394, 770
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 5 cut(s) 49, 111, 168, 217, 386
Hpy188III TCNNGA 2 cut(s) 62, 299
Hpy8I GTNNAC 1 cut(s) 418
HpyAV CCTTC 1 cut(s) 681
HpyCH4III ACNGT 3 cut(s) 69, 201, 522
HpyCH4IV ACGT 1 cut(s) 693
HpyCH4V TGCA 4 cut(s) 18, 129, 272, 306
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 126
HpyF3I CTNAG 3 cut(s) 92, 110, 165
HpySE526I ACGT 1 cut(s) 693
Hsp92II CATG 3 cut(s) 22, 269, 411
HspAI GCGC 1 cut(s) 394
KroI GCCGGC 1 cut(s) 509
KroNI GCCGGC 1 cut(s) 511
Ksp22I TGATCA 1 cut(s) 217
KspAI GTTAAC 1 cut(s) 418
Kzo9I GATC 6 cut(s) 49, 103, 217, 381, 486, 744
LmnI GCTCC 2 cut(s) 346, 368
Lsp1109I GCAGC 4 cut(s) 284, 493, 682, 685
LweI GCATC 1 cut(s) 599
MaeII ACGT 1 cut(s) 693
MaeIII GTNAC 1 cut(s) 499
MalI GATC 6 cut(s) 51, 105, 219, 383, 488, 746
MboI GATC 6 cut(s) 49, 103, 217, 381, 486, 744
MboII GAAGA 7 cut(s) 44, 47, 98, 141, 168, 350, 623
MflI RGATCY 2 cut(s) 381, 744
MhlI GDGCHC 1 cut(s) 725
MluCI AATT 6 cut(s) 397, 423, 452, 618, 699, 774
MmeI TCCRAC 1 cut(s) 409
MnlI CCTC 7 cut(s) 69, 199, 259, 297, 467, 628, 715
MroNI GCCGGC 1 cut(s) 509
MseI TTAA 3 cut(s) 338, 417, 732
MslI CAYNNNNRTG 1 cut(s) 256
MspA1I CMGCKG 2 cut(s) 608, 676
MspI CCGG 3 cut(s) 510, 557, 563
MspR9I CCNGG 2 cut(s) 190, 491
MvaI CCWGG 2 cut(s) 190, 491
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 2 cut(s) 24, 126
NaeI GCCGGC 1 cut(s) 511
NdeI CATATG 1 cut(s) 181
NdeII GATC 6 cut(s) 49, 103, 217, 381, 486, 744
NgoMIV GCCGGC 1 cut(s) 509
NlaIII CATG 3 cut(s) 22, 269, 411
NlaIV GGNNCC 6 cut(s) 370, 383, 555, 561, 567, 746
NmuCI GTSAC 1 cut(s) 499
NspI RCATGY 1 cut(s) 269
PdiI GCCGGC 1 cut(s) 511
PfeI GAWTC 2 cut(s) 173, 756
PflMI CCANNNNNTGG 1 cut(s) 378
PkrI GCNGC 5 cut(s) 39, 274, 508, 672, 675
Psp6I CCWGG 2 cut(s) 188, 489
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 2 cut(s) 188, 489
PspN4I GGNNCC 6 cut(s) 370, 383, 555, 561, 567, 746
PstNI CAGNNNCTG 2 cut(s) 126, 676
PsuI RGATCY 2 cut(s) 381, 744
PvuII CAGCTG 2 cut(s) 608, 676
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 256
SaqAI TTAA 3 cut(s) 338, 417, 732
SatI GCNGC 5 cut(s) 38, 273, 507, 671, 674
Sau3AI GATC 6 cut(s) 49, 103, 217, 381, 486, 744
ScrFI CCNGG 2 cut(s) 190, 491
SduI GDGCHC 1 cut(s) 725
SetI ASST 9 cut(s) 160, 166, 351, 485, 495, 519, 610, 678, 696
SfaNI GCATC 1 cut(s) 599
SfcI CTRYAG 1 cut(s) 518
SmiMI CAYNNNNRTG 1 cut(s) 256
Sse9I AATT 6 cut(s) 397, 423, 452, 618, 699, 774
SsiI CCGC 5 cut(s) 35, 38, 118, 366, 471
StyD4I CCNGG 2 cut(s) 188, 489
TaaI ACNGT 3 cut(s) 69, 201, 522
TaiI ACGT 1 cut(s) 696
TaqI TCGA 1 cut(s) 102
TasI AATT 6 cut(s) 397, 423, 452, 618, 699, 774
TauI GCSGC 1 cut(s) 40
TfiI GAWTC 2 cut(s) 173, 756
Tru1I TTAA 3 cut(s) 338, 417, 732
Tru9I TTAA 3 cut(s) 338, 417, 732
TscAI CASTG 4 cut(s) 127, 508, 527, 533
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 4 cut(s) 272, 506, 670, 673
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 7 cut(s) 168, 186, 198, 270, 350, 390, 630
TspRI CASTG 4 cut(s) 127, 508, 527, 533
Van91I CCANNNNNTGG 1 cut(s) 378
XapI RAATTY 1 cut(s) 452
XceI RCATGY 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.