Rh4AG099200

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001757
Physical Location & Seq
Reverse (-)
21299555 .. 21300828
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG099200.1

Sequence Viewer

Length: 996 bp
ATGTCTATCGAAGAAACGAAAACTAGGGAATGGTCTAATCTTCCTACTGAACTTCTGGAATGCATAGCAGATCGTGCAGGCCTATTCGAGCTTCCAAGTATCTATCTTGTCTGCAAGGCTTGGTATTCTGCTTGTTCTACAACTTTAGCTCACATTGTTAAGCCTGAATTAAACCAAGGAGCTTGGTTTCTACTATATGAGGATAAAAGTTCTCAGTGCCAATTGATAACTGCAAGTGGTAGGAAGTTCACCACGAGTCTCCCTGAACTGGTTGGAACAACTTGCCTTGCAACATACCAAGCATGGCTTCTTCTTTTCAAAGGAGGATGTGCTTCAATGTTCTTCTTTCAACCTTTTTCTCGCCAAAGAATAGACCTCCCAGGGCTCCCATTAGCGGAACTCACTGATCATCGTTTTGCAGCAGTTTCATGTCCACCAACTTTCAAAGATTGCATGGTTTGTGTGATCAACCGTAGGAATATTTATGAATGGGACGCCAACTTACTTTACCTTGGAAACCAGGCTTGGGTTGGAACACAGTGTAATCTTTCCCACACATGCCCGAATACAATCAAATGCGCTGTCTATGAAAATCAAACATTCAGATTTTACGATATCGTTCTAGGTCGACACATGCGCACCATGCTCGTCATATATCCTGACGGACGTTTCGCCTGGAATAAACCTTCAAAAGTAGTAGGTCGTTACGTACCACAGCATTTCCGGGACCAGAAAATACATCGTGATTTATTGATTTTTGTTTCTGGCAAGAATGAGTTGAAGAAAAAGTTGAAGGACATGAAGAACAAGCTGAGGTTGACAGAGGACGTTGTTTCTACTTGTGGGACATTTACACAGTTAGATGGCAAGCGCAAGCTTATTTTTAATGAGAGGCTCATGGATCCTGTTGACCAAAGTTTCAATGGAGTATGGACTGTGTGGATCCAGCCAAGGTTCTCTATCTCCACATTAGATGATGAAGCTCACGCACCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

331

Amino Acids

38.06

Weight (kDa)

8.62

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 83 - 239 5.9e-12 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 638
AccI GTMKAC 1 cut(s) 628
AciI CCGC 1 cut(s) 395
AclWI GGATC 4 cut(s) 896, 909, 937, 950
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 1 cut(s) 711
AfiI CCNNNNNNNGG 3 cut(s) 268, 394, 526
AgsI TTSAA 8 cut(s) 319, 336, 350, 445, 690, 781, 793, 922
AjnI CCWGG 3 cut(s) 379, 519, 674
AjuI GAANNNNNNNTTGG 4 cut(s) 291, 323, 508, 540
AluBI AGCT 6 cut(s) 91, 149, 182, 811, 877, 983
AluI AGCT 6 cut(s) 91, 149, 182, 811, 877, 983
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 4 cut(s) 896, 909, 937, 950
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 419
ArsI GACNNNNNNTTYG 4 cut(s) 567, 599, 653, 685
AspLEI GCGC 3 cut(s) 581, 639, 873
AspS9I GGNCC 1 cut(s) 727
AsuC2I CCSGG 1 cut(s) 725
AsuHPI GGTGA 1 cut(s) 241
AvaII GGWCC 1 cut(s) 727
BamHI GGATCC 2 cut(s) 901, 942
BanII GRGCYC 1 cut(s) 387
BauI CACGAG 1 cut(s) 253
BbvCI CCTCAGC 1 cut(s) 812
BbvI GCAGC 1 cut(s) 431
BccI CCATC 1 cut(s) 857
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BciT130I CCWGG 3 cut(s) 381, 521, 676
BclI TGATCA 2 cut(s) 406, 465
BcnI CCSGG 1 cut(s) 725
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 2 cut(s) 24, 623
BisI GCNGC 1 cut(s) 420
BlsI GCNGC 1 cut(s) 421
Bme1390I CCNGG 4 cut(s) 381, 521, 676, 725
Bme18I GGWCC 1 cut(s) 727
BmgT120I GGNCC 1 cut(s) 727
BmiI GGNNCC 4 cut(s) 386, 728, 903, 944
BmrFI CCNGG 4 cut(s) 381, 521, 676, 725
Bpu10I CCTNAGC 1 cut(s) 812
BpuMI CCSGG 1 cut(s) 725
BsaAI YACGTR 1 cut(s) 709
BsaHI GRCGYC 1 cut(s) 495
BsaJI CCNNGG 5 cut(s) 175, 379, 380, 511, 950
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 394, 526
Bse1I ACTGG 1 cut(s) 273
BseBI CCWGG 3 cut(s) 381, 521, 676
BseDI CCNNGG 5 cut(s) 175, 379, 380, 511, 950
BseGI GGATG 1 cut(s) 332
BseLI CCNNNNNNNGG 3 cut(s) 268, 394, 526
BseMII CTCAG 2 cut(s) 227, 803
BseNI ACTGG 1 cut(s) 273
BseXI GCAGC 1 cut(s) 431
BsgI GTGCAG 1 cut(s) 96
BshFI GGCC 1 cut(s) 81
BsiSI CCGG 1 cut(s) 724
BslFI GGGAC 3 cut(s) 506, 740, 859
BslI CCNNNNNNNGG 3 cut(s) 268, 394, 526
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 3 cut(s) 506, 740, 859
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 5 cut(s) 70, 406, 465, 901, 942
BspACI CCGC 1 cut(s) 395
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 2 cut(s) 226, 804
BspLI GGNNCC 4 cut(s) 386, 728, 903, 944
BspPI GGATC 4 cut(s) 896, 909, 937, 950
BsrI ACTGG 1 cut(s) 273
BssECI CCNNGG 5 cut(s) 175, 379, 380, 511, 950
BssMI GATC 5 cut(s) 70, 406, 465, 901, 942
BssNI GRCGYC 1 cut(s) 495
BssSI CACGAG 1 cut(s) 253
BssT1I CCWWGG 3 cut(s) 175, 511, 950
Bst2BI CACGAG 1 cut(s) 253
Bst2UI CCWGG 3 cut(s) 381, 521, 676
Bst4CI ACNGT 5 cut(s) 473, 540, 858, 937, 993
BstACI GRCGYC 1 cut(s) 495
BstAPI GCANNNNNTGC 1 cut(s) 74
BstBAI YACGTR 1 cut(s) 709
BstC8I GCNNGC 3 cut(s) 79, 869, 875
BstDEI CTNAG 2 cut(s) 213, 812
BstF5I GGATG 1 cut(s) 332
BstHHI GCGC 3 cut(s) 581, 639, 873
BstKTI GATC 5 cut(s) 73, 409, 468, 904, 945
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 5 cut(s) 70, 406, 465, 901, 942
BstMWI GCNNNNNNNGC 2 cut(s) 74, 643
BstNI CCWGG 3 cut(s) 381, 521, 676
BstNSI RCATGY 2 cut(s) 561, 637
BstSCI CCNGG 4 cut(s) 379, 519, 674, 723
BstSNI TACGTA 1 cut(s) 709
BstV1I GCAGC 1 cut(s) 431
BstX2I RGATCY 2 cut(s) 901, 942
BstYI RGATCY 2 cut(s) 901, 942
BsuRI GGCC 1 cut(s) 81
BtsCI GGATG 1 cut(s) 332
BtsIMutI CAGTG 3 cut(s) 221, 402, 545
Cac8I GCNNGC 3 cut(s) 79, 869, 875
CfoI GCGC 3 cut(s) 581, 639, 873
Cfr13I GGNCC 1 cut(s) 727
CseI GACGC 1 cut(s) 503
Csp6I GTAC 1 cut(s) 710
CviAII CATG 8 cut(s) 303, 429, 454, 558, 634, 643, 799, 898
CviQI GTAC 1 cut(s) 710
DdeI CTNAG 2 cut(s) 213, 812
DpnI GATC 5 cut(s) 72, 408, 467, 903, 944
DpnII GATC 5 cut(s) 70, 406, 465, 901, 942
Eco105I TACGTA 1 cut(s) 709
Eco130I CCWWGG 3 cut(s) 175, 511, 950
Eco147I AGGCCT 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 387
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 1 cut(s) 727
EcoRII CCWGG 3 cut(s) 379, 519, 674
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 3 cut(s) 175, 511, 950
EcoT22I ATGCAT 1 cut(s) 65
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 3 cut(s) 175, 511, 950
FaeI CATG 8 cut(s) 306, 432, 457, 561, 637, 646, 802, 901
FalI AAGNNNNNCTT 2 cut(s) 291, 323
FaqI GGGAC 3 cut(s) 506, 740, 859
FatI CATG 8 cut(s) 302, 428, 453, 557, 633, 642, 798, 897
FbaI TGATCA 2 cut(s) 406, 465
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 1 cut(s) 420
FokI GGATG 1 cut(s) 339
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 1 cut(s) 420
FspAI RTGCGCAY 1 cut(s) 638
FspBI CTAG 2 cut(s) 24, 623
FspI TGCGCA 1 cut(s) 638
GlaI GCGC 3 cut(s) 580, 638, 872
GluI GCNGC 1 cut(s) 420
HaeIII GGCC 1 cut(s) 81
HapII CCGG 1 cut(s) 724
HgaI GACGC 1 cut(s) 503
HhaI GCGC 3 cut(s) 581, 639, 873
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 8 cut(s) 306, 432, 457, 561, 637, 646, 802, 901
Hin6I GCGC 3 cut(s) 579, 637, 871
HinP1I GCGC 3 cut(s) 579, 637, 871
HincII GTYRAC 3 cut(s) 629, 819, 910
HindII GTYRAC 3 cut(s) 629, 819, 910
HindIII AAGCTT 1 cut(s) 875
HinfI GANTC 1 cut(s) 256
HpaII CCGG 1 cut(s) 724
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 5 cut(s) 249, 434, 629, 819, 910
Hpy188I TCNGA 1 cut(s) 605
Hpy188III TCNNGA 3 cut(s) 56, 659, 743
Hpy8I GTNNAC 5 cut(s) 249, 434, 629, 819, 910
HpyAV CCTTC 2 cut(s) 696, 787
HpyCH4III ACNGT 5 cut(s) 473, 540, 858, 937, 993
HpyCH4IV ACGT 3 cut(s) 667, 708, 828
HpyCH4V TGCA 7 cut(s) 63, 77, 114, 233, 290, 419, 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 643
HpyF3I CTNAG 2 cut(s) 213, 812
HpySE526I ACGT 3 cut(s) 667, 708, 828
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 8 cut(s) 306, 432, 457, 561, 637, 646, 802, 901
HspAI GCGC 3 cut(s) 579, 637, 871
Ksp22I TGATCA 2 cut(s) 406, 465
Kzo9I GATC 5 cut(s) 70, 406, 465, 901, 942
LmnI GCTCC 2 cut(s) 179, 390
Lsp1109I GCAGC 1 cut(s) 431
MaeI CTAG 2 cut(s) 24, 623
MaeII ACGT 3 cut(s) 667, 708, 828
MaeIII GTNAC 1 cut(s) 704
MalI GATC 5 cut(s) 72, 408, 467, 903, 944
MboI GATC 5 cut(s) 70, 406, 465, 901, 942
MboII GAAGA 6 cut(s) 23, 32, 302, 334, 793, 814
MfeI CAATTG 1 cut(s) 221
MflI RGATCY 2 cut(s) 901, 942
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 2 cut(s) 167, 221
MlyI GAGTC 1 cut(s) 265
MmeI TCCRAC 2 cut(s) 253, 511
MnlI CCTC 6 cut(s) 193, 317, 386, 807, 817, 885
Mph1103I ATGCAT 1 cut(s) 65
MseI TTAA 3 cut(s) 159, 170, 885
MspI CCGG 1 cut(s) 724
MspR9I CCNGG 4 cut(s) 381, 521, 676, 725
MunI CAATTG 1 cut(s) 221
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 3 cut(s) 381, 521, 676
MwoI GCNNNNNNNGC 2 cut(s) 74, 643
NciI CCSGG 1 cut(s) 725
NdeII GATC 5 cut(s) 70, 406, 465, 901, 942
NlaIII CATG 8 cut(s) 306, 432, 457, 561, 637, 646, 802, 901
NlaIV GGNNCC 4 cut(s) 386, 728, 903, 944
NsbI TGCGCA 1 cut(s) 638
NsiI ATGCAT 1 cut(s) 65
NspI RCATGY 2 cut(s) 561, 637
PasI CCCWGGG 1 cut(s) 380
PceI AGGCCT 1 cut(s) 81
PctI GAATGC 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 723
PkrI GCNGC 1 cut(s) 421
PleI GAGTC 1 cut(s) 264
PpsI GAGTC 1 cut(s) 264
Ppu21I YACGTR 1 cut(s) 709
Psp6I CCWGG 3 cut(s) 379, 519, 674
PspGI CCWGG 3 cut(s) 379, 519, 674
PspN4I GGNNCC 4 cut(s) 386, 728, 903, 944
PspPI GGNCC 1 cut(s) 727
PsuI RGATCY 2 cut(s) 901, 942
RsaI GTAC 1 cut(s) 711
RsaNI GTAC 1 cut(s) 710
SalI GTCGAC 1 cut(s) 627
SaqAI TTAA 3 cut(s) 159, 170, 885
SatI GCNGC 1 cut(s) 420
Sau3AI GATC 5 cut(s) 70, 406, 465, 901, 942
Sau96I GGNCC 1 cut(s) 727
SchI GAGTC 1 cut(s) 265
ScrFI CCNGG 4 cut(s) 381, 521, 676, 725
SduI GDGCHC 1 cut(s) 387
SinI GGWCC 1 cut(s) 727
SnaBI TACGTA 1 cut(s) 709
Sse9I AATT 2 cut(s) 167, 221
SseBI AGGCCT 1 cut(s) 81
SsiI CCGC 1 cut(s) 395
SspI AATATT 1 cut(s) 481
SspMI CTAG 2 cut(s) 24, 623
StuI AGGCCT 1 cut(s) 81
StyD4I CCNGG 4 cut(s) 379, 519, 674, 723
StyI CCWWGG 3 cut(s) 175, 511, 950
TaaI ACNGT 5 cut(s) 473, 540, 858, 937, 993
TaiI ACGT 3 cut(s) 670, 711, 831
TaqI TCGA 3 cut(s) 9, 87, 628
TasI AATT 2 cut(s) 167, 221
Tru1I TTAA 3 cut(s) 159, 170, 885
Tru9I TTAA 3 cut(s) 159, 170, 885
TscAI CASTG 3 cut(s) 221, 409, 545
TseI GCWGC 1 cut(s) 419
TspDTI ATGAA 5 cut(s) 417, 501, 603, 815, 993
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 3 cut(s) 221, 409, 545
VpaK11BI GGWCC 1 cut(s) 727
XceI RCATGY 2 cut(s) 561, 637
XcmI CCANNNNNNNNNTGG 2 cut(s) 527, 920
XmiI GTMKAC 1 cut(s) 628
XspI CTAG 2 cut(s) 24, 623
Zsp2I ATGCAT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.