FvH4_2g24410

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001757
Physical Location & Seq
Forward (+)
19934407 .. 19936348
1942 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24410.t1

Sequence Viewer

Length: 1044 bp
ATGCATTGTAGAGAGGAAATGACTAAGGAGCCAAGGCGAAATAGGGACTGGTCCGATCTCCCTCAGGAACTTGTAGAAAAAATAGCAGAACCTGCAGGCCTATTCGAGCTTCTGAGTTTATTTCGTGTCTGCAATGCTTGGCGCTCTACTTCCACCGCTTTGGCTCAAATGGTTAAGCATGAATTAAACCAAGGACCTTGGTTTCTACTATATGAGGATAATAATCCTGAGTGCCAAATGCTAACTGCAAGTGGTAAGAAGTTCATCACAAGTCTACCGGAACTGAATGGAACCACTTGCCTTGCATCATACCATGGATGGCTTCTTTTGTTCCGACAAGGAGGTACCATATTCTTCTTTCACGTCTTCTCGCGCCAGAGAATAGACCTTCCTGAATTCCCAGTTCCAGAACTCACTGATCATGTTGGAGCAGTATCATCGTCTCCAACTTCCCAAGATTGTGTGGTTTGTGTCATTAGCTGTAGCGACGATGAAACCTTGGATGCCAAGGTACTTCACCTTAGAGACCAGACTTGGATTGGATCAGAGTACTCTCATCCTCGAGCTAAAAAAGACAAAATCAAATGTGGTATTTATTCCACTACTGGGAAGGGGATGTTCGGCTTTTTGGATGGAGTTGATTTCTTGACCTCGATCGCCATATCTGCTGAAAACCGCTCTCTCATTTGGAATTTTCAATCCATAACAGCCGCAAAAACTGAGGAAATTTCCGATCCATCACTATCAAAAAGAAACACTTACCATCTCTGTCCAGGTGAGTTGGAGATTAGGTTCAAGGACCTGAAAAACAAGCTGGGGTTGACAGCGGATGTTACGATTTCTAGTTGTGGGACAATTACACCATGCAGTATTGGCGGTGGGAATTACAGGGTTCTTTATAATGAGAGCATCAGCGACAATTATATTGACAAATCTAAGAACCGTTGTTTCAAAGGGGTATTGATCCAACCAAGATCCTCGATACATAGAAGAGATATGAAAGCTCATTCTCGAGGTACTACTGCTACTGATCGATCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

348

Amino Acids

39.06

Weight (kDa)

6.79

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 88 - 184 2.7e-12 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 900
Acc36I ACCTGC 1 cut(s) 100
Acc65I GGTACC 1 cut(s) 344
AccB1I GGYRCC 1 cut(s) 344
AccBSI CCGCTC 1 cut(s) 678
AccI GTMKAC 1 cut(s) 274
AccII CGCG 1 cut(s) 373
AciI CCGC 5 cut(s) 156, 676, 711, 827, 876
AclWI GGATC 4 cut(s) 550, 728, 958, 969
AcsI RAATTY 3 cut(s) 395, 691, 726
AfaI GTAC 4 cut(s) 346, 513, 551, 1018
AfiI CCNNNNNNNGG 1 cut(s) 606
AgsI TTSAA 3 cut(s) 698, 796, 952
AjiI CACGTC 1 cut(s) 364
AjnI CCWGG 1 cut(s) 772
AloI GAACNNNNNNTCC 2 cut(s) 776, 808
AluBI AGCT 5 cut(s) 109, 480, 566, 814, 1004
AluI AGCT 5 cut(s) 109, 480, 566, 814, 1004
Alw26I GTCTC 2 cut(s) 447, 519
AlwI GGATC 4 cut(s) 550, 728, 958, 969
AlwNI CAGNNNCTG 1 cut(s) 92
Ama87I CYCGRG 2 cut(s) 561, 1011
AoxI GGCC 1 cut(s) 97
ApoI RAATTY 3 cut(s) 395, 691, 726
Asp718I GGTACC 1 cut(s) 344
AspLEI GCGC 2 cut(s) 144, 375
AspS9I GGNCC 3 cut(s) 51, 194, 799
AsuHPI GGTGA 2 cut(s) 509, 788
AvaI CYCGRG 2 cut(s) 561, 1011
AvaII GGWCC 3 cut(s) 51, 194, 799
AxyI CCTNAGG 1 cut(s) 63
BanI GGYRCC 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 358
BccI CCATC 4 cut(s) 312, 626, 745, 771
BcgI CGANNNNNNTGC 2 cut(s) 420, 454
BciT130I CCWGG 1 cut(s) 774
BclI TGATCA 1 cut(s) 418
BcoDI GTCTC 2 cut(s) 447, 519
BfaI CTAG 1 cut(s) 843
BfmI CTRYAG 2 cut(s) 93, 481
BfoI RGCGCY 1 cut(s) 145
BfuAI ACCTGC 1 cut(s) 100
BisI GCNGC 1 cut(s) 711
BlsI GCNGC 1 cut(s) 712
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 1 cut(s) 774
Bme18I GGWCC 3 cut(s) 51, 194, 799
BmeT110I CYCGRG 2 cut(s) 561, 1011
BmgBI CACGTC 1 cut(s) 364
BmgT120I GGNCC 3 cut(s) 51, 194, 799
BmiI GGNNCC 3 cut(s) 30, 292, 346
BmrFI CCNGG 1 cut(s) 774
BmrI ACTGGG 2 cut(s) 395, 615
BmsI GCATC 3 cut(s) 314, 493, 918
BmuI ACTGGG 2 cut(s) 395, 615
BpiI GAAGAC 1 cut(s) 358
Bsa29I ATCGAT 1 cut(s) 1033
BsaBI GATNNNNATC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 519
BsaJI CCNNGG 6 cut(s) 32, 190, 197, 313, 498, 507
BsaWI WCCGGW 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 776, 806
Bsc4I CCNNNNNNNGG 1 cut(s) 606
Bse1I ACTGG 3 cut(s) 53, 401, 610
Bse21I CCTNAGG 1 cut(s) 63
Bse3DI GCAATG 1 cut(s) 139
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 1 cut(s) 774
BseCI ATCGAT 1 cut(s) 1033
BseDI CCNNGG 6 cut(s) 32, 190, 197, 313, 498, 507
BseGI GGATG 6 cut(s) 323, 508, 556, 621, 637, 835
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 606
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 4 cut(s) 77, 104, 219, 711
BseNI ACTGG 3 cut(s) 53, 401, 610
BseYI CCCAGC 1 cut(s) 814
Bsh1236I CGCG 1 cut(s) 373
Bsh1285I CGRYCG 1 cut(s) 657
BshFI GGCC 1 cut(s) 99
BshNI GGYRCC 1 cut(s) 344
BshVI ATCGAT 1 cut(s) 1033
BsiEI CGRYCG 1 cut(s) 657
BsiHKCI CYCGRG 2 cut(s) 561, 1011
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 2 cut(s) 59, 865
BslI CCNNNNNNNGG 1 cut(s) 606
BsmAI GTCTC 2 cut(s) 447, 519
BsmBI CGTCTC 1 cut(s) 447
BsmFI GGGAC 2 cut(s) 59, 865
BsnI GGCC 1 cut(s) 99
Bso31I GGTCTC 1 cut(s) 519
BsoBI CYCGRG 2 cut(s) 561, 1011
Bsp143I GATC 9 cut(s) 55, 418, 542, 654, 733, 963, 974, 1030, 1034
Bsp19I CCATGG 1 cut(s) 313
BspACI CCGC 5 cut(s) 156, 676, 711, 827, 876
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 4 cut(s) 76, 105, 220, 712
BspDI ATCGAT 1 cut(s) 1033
BspFNI CGCG 1 cut(s) 373
BspLI GGNNCC 3 cut(s) 30, 292, 346
BspMAI CTGCAG 1 cut(s) 97
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 4 cut(s) 550, 728, 958, 969
BspT107I GGYRCC 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 519
BsrBI CCGCTC 1 cut(s) 678
BsrDI GCAATG 1 cut(s) 139
BsrI ACTGG 3 cut(s) 53, 401, 610
BssECI CCNNGG 6 cut(s) 32, 190, 197, 313, 498, 507
BssMI GATC 9 cut(s) 55, 418, 542, 654, 733, 963, 974, 1030, 1034
BssT1I CCWWGG 6 cut(s) 32, 190, 197, 313, 498, 507
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 1 cut(s) 944
Bst6I CTCTTC 1 cut(s) 985
BstAPI GCANNNNNTGC 1 cut(s) 92
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 7 cut(s) 24, 63, 113, 228, 521, 720, 936
BstDSI CCRYGG 1 cut(s) 313
BstF5I GGATG 6 cut(s) 323, 508, 556, 621, 637, 835
BstFNI CGCG 1 cut(s) 373
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 2 cut(s) 144, 375
BstKTI GATC 9 cut(s) 58, 421, 545, 657, 736, 966, 977, 1033, 1037
BstMAI GTCTC 2 cut(s) 447, 519
BstMBI GATC 9 cut(s) 55, 418, 542, 654, 733, 963, 974, 1030, 1034
BstMCI CGRYCG 1 cut(s) 657
BstMWI GCNNNNNNNGC 3 cut(s) 92, 665, 873
BstNI CCWGG 1 cut(s) 774
BstSCI CCNGG 1 cut(s) 772
BstSFI CTRYAG 2 cut(s) 93, 481
BstUI CGCG 1 cut(s) 373
BstV2I GAAGAC 1 cut(s) 358
BstX2I RGATCY 1 cut(s) 974
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 1 cut(s) 974
Bsu15I ATCGAT 1 cut(s) 1033
Bsu36I CCTNAGG 1 cut(s) 63
BsuRI GGCC 1 cut(s) 99
BsuTUI ATCGAT 1 cut(s) 1033
BtgI CCRYGG 1 cut(s) 313
BtrI CACGTC 1 cut(s) 364
BtsCI GGATG 6 cut(s) 323, 508, 556, 621, 637, 835
BtsIMutI CAGTG 1 cut(s) 414
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 97
CaiI CAGNNNCTG 1 cut(s) 92
CfoI GCGC 2 cut(s) 144, 375
Cfr13I GGNCC 3 cut(s) 51, 194, 799
ClaI ATCGAT 1 cut(s) 1033
Csp6I GTAC 4 cut(s) 345, 512, 550, 1017
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 4 cut(s) 179, 314, 422, 864
CviQI GTAC 4 cut(s) 345, 512, 550, 1017
DdeI CTNAG 7 cut(s) 24, 63, 113, 228, 521, 720, 936
DpnI GATC 9 cut(s) 57, 420, 544, 656, 735, 965, 976, 1032, 1036
DpnII GATC 9 cut(s) 55, 418, 542, 654, 733, 963, 974, 1030, 1034
Eam1104I CTCTTC 1 cut(s) 985
EarI CTCTTC 1 cut(s) 985
Eco130I CCWWGG 6 cut(s) 32, 190, 197, 313, 498, 507
Eco147I AGGCCT 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 519
Eco47I GGWCC 3 cut(s) 51, 194, 799
Eco81I CCTNAGG 1 cut(s) 63
Eco88I CYCGRG 2 cut(s) 561, 1011
EcoO109I RGGNCCY 2 cut(s) 194, 799
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 772
EcoT14I CCWWGG 6 cut(s) 32, 190, 197, 313, 498, 507
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 6 cut(s) 32, 190, 197, 313, 498, 507
Esp3I CGTCTC 1 cut(s) 447
FaeI CATG 4 cut(s) 182, 317, 425, 867
FalI AAGNNNNNCTT 2 cut(s) 742, 774
FaqI GGGAC 2 cut(s) 59, 865
FatI CATG 4 cut(s) 178, 313, 421, 863
FbaI TGATCA 1 cut(s) 418
FblI GTMKAC 1 cut(s) 274
Fnu4HI GCNGC 1 cut(s) 711
FokI GGATG 6 cut(s) 330, 515, 543, 628, 644, 842
Fsp4HI GCNGC 1 cut(s) 711
FspBI CTAG 1 cut(s) 843
GlaI GCGC 2 cut(s) 143, 374
GluI GCNGC 1 cut(s) 711
GsaI CCCAGC 1 cut(s) 818
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 1 cut(s) 99
HapII CCGG 1 cut(s) 278
HhaI GCGC 2 cut(s) 144, 375
Hin1II CATG 4 cut(s) 182, 317, 425, 867
Hin6I GCGC 2 cut(s) 142, 373
HinP1I GCGC 2 cut(s) 142, 373
HincII GTYRAC 1 cut(s) 822
HindII GTYRAC 1 cut(s) 822
HpaII CCGG 1 cut(s) 278
HphI GGTGA 2 cut(s) 509, 788
Hpy166II GTNNAC 2 cut(s) 275, 822
Hpy188I TCNGA 6 cut(s) 55, 114, 335, 547, 733, 1039
Hpy188III TCNNGA 6 cut(s) 65, 227, 392, 407, 646, 1011
Hpy8I GTNNAC 2 cut(s) 275, 822
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 2 cut(s) 398, 604
HpyCH4III ACNGT 1 cut(s) 944
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 6 cut(s) 4, 95, 132, 248, 305, 867
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 665, 873
HpyF3I CTNAG 7 cut(s) 24, 63, 113, 228, 521, 720, 936
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 4 cut(s) 182, 317, 425, 867
HspAI GCGC 2 cut(s) 142, 373
KpnI GGTACC 1 cut(s) 348
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 9 cut(s) 55, 418, 542, 654, 733, 963, 974, 1030, 1034
LmnI GCTCC 2 cut(s) 28, 428
LweI GCATC 3 cut(s) 314, 493, 918
MaeI CTAG 1 cut(s) 843
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 832
MalI GATC 9 cut(s) 57, 420, 544, 656, 735, 965, 976, 1032, 1036
MbiI CCGCTC 1 cut(s) 678
MboI GATC 9 cut(s) 55, 418, 542, 654, 733, 963, 974, 1030, 1034
MboII GAAGA 3 cut(s) 346, 358, 1002
MflI RGATCY 1 cut(s) 974
MluCI AATT 7 cut(s) 182, 395, 691, 726, 855, 883, 919
MmeI TCCRAC 5 cut(s) 358, 406, 470, 762, 991
MnlI CCTC 9 cut(s) 7, 72, 208, 335, 570, 661, 715, 988, 1007
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 174, 185
MspA1I CMGCKG 1 cut(s) 827
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 774
MvaI CCWGG 1 cut(s) 774
MvnI CGCG 1 cut(s) 373
MwoI GCNNNNNNNGC 3 cut(s) 92, 665, 873
NcoI CCATGG 1 cut(s) 313
NdeII GATC 9 cut(s) 55, 418, 542, 654, 733, 963, 974, 1030, 1034
NlaIII CATG 4 cut(s) 182, 317, 425, 867
NlaIV GGNNCC 3 cut(s) 30, 292, 346
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 2 cut(s) 561, 1011
PceI AGGCCT 1 cut(s) 99
PkrI GCNGC 1 cut(s) 712
Ple19I CGATCG 1 cut(s) 657
PpuMI RGGWCCY 2 cut(s) 194, 799
PsiI TTATAA 1 cut(s) 900
Psp5II RGGWCCY 2 cut(s) 194, 799
Psp6I CCWGG 1 cut(s) 772
PspFI CCCAGC 1 cut(s) 814
PspGI CCWGG 1 cut(s) 772
PspN4I GGNNCC 3 cut(s) 30, 292, 346
PspPI GGNCC 3 cut(s) 51, 194, 799
PspPPI RGGWCCY 2 cut(s) 194, 799
PspXI VCTCGAGB 1 cut(s) 561
PstI CTGCAG 1 cut(s) 97
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 1 cut(s) 974
PvuI CGATCG 1 cut(s) 657
RsaI GTAC 4 cut(s) 346, 513, 551, 1018
RsaNI GTAC 4 cut(s) 345, 512, 550, 1017
SaqAI TTAA 2 cut(s) 174, 185
SatI GCNGC 1 cut(s) 711
Sau3AI GATC 9 cut(s) 55, 418, 542, 654, 733, 963, 974, 1030, 1034
Sau96I GGNCC 3 cut(s) 51, 194, 799
SbfI CCTGCAGG 1 cut(s) 97
ScaI AGTACT 1 cut(s) 551
ScrFI CCNGG 1 cut(s) 774
SdaI CCTGCAGG 1 cut(s) 97
SfaNI GCATC 3 cut(s) 314, 493, 918
SfcI CTRYAG 2 cut(s) 93, 481
Sfr274I CTCGAG 2 cut(s) 561, 1011
SinI GGWCC 3 cut(s) 51, 194, 799
SlaI CTCGAG 2 cut(s) 561, 1011
SmlI CTYRAG 2 cut(s) 561, 1011
SmoI CTYRAG 2 cut(s) 561, 1011
Sse8387I CCTGCAGG 1 cut(s) 97
Sse9I AATT 7 cut(s) 182, 395, 691, 726, 855, 883, 919
SseBI AGGCCT 1 cut(s) 99
SsiI CCGC 5 cut(s) 156, 676, 711, 827, 876
SspMI CTAG 1 cut(s) 843
StuI AGGCCT 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 772
StyI CCWWGG 6 cut(s) 32, 190, 197, 313, 498, 507
TaaI ACNGT 1 cut(s) 944
TaiI ACGT 1 cut(s) 366
TaqI TCGA 6 cut(s) 105, 562, 653, 980, 1012, 1033
TasI AATT 7 cut(s) 182, 395, 691, 726, 855, 883, 919
TatI WGTACW 1 cut(s) 549
TauI GCSGC 1 cut(s) 713
Tru1I TTAA 2 cut(s) 174, 185
Tru9I TTAA 2 cut(s) 174, 185
TscAI CASTG 1 cut(s) 421
TspDTI ATGAA 4 cut(s) 195, 253, 507, 1013
TspRI CASTG 1 cut(s) 421
VpaK11BI GGWCC 3 cut(s) 51, 194, 799
XapI RAATTY 3 cut(s) 395, 691, 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XhoI CTCGAG 2 cut(s) 561, 1011
XmiI GTMKAC 1 cut(s) 274
XspI CTAG 1 cut(s) 843
ZrmI AGTACT 1 cut(s) 551
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.