Rh6BG345300

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001757
Physical Location & Seq
Forward (+)
57705274 .. 57705720
447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG345300.1

Sequence Viewer

Length: 447 bp
ATGTTCTTCTTCCACGTCTTCTCTGGCCAGAGAATAGACCTTCCTGAATTTCCAGTCCCGGAAGTACTCACTGATCATGTTGCAGCAGTTTCATGTCCTCCCTCGACAACTTCCCAAGATTGCGTGGTTTGTGTCATTAGCCGCAGCAGGGATGCTGAATTGGAAGCCAAGGTACTTCACCTTGGAGAAGATCAGGCTTGGATTGAATCAAAGCATTCTCGTTCCCGAGTTCACACCGATACAATCAAATGTGCTGTTTATCACACTGGAATGGGAATGTTCTGCTTTTTCGATAGAGCTGCTTCCAGTACCATGATCACCATATCTACTGAAAAGCGACCTATCGAATGGAAATGTATTGAATTCGGACCCGCAAAAACTGAGGAAAATGTCGAGCCATCAAGATCAAAATATTTTACTCTTGTCATGCACTCATGCATAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

148

Amino Acids

16.72

Weight (kDa)

5.95

Isoelectric Point (pI)

67.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 1 - 118 5.6e-07 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 142, 372
AcoI YGGCCR 1 cut(s) 25
AcsI RAATTY 2 cut(s) 47, 362
AfaI GTAC 3 cut(s) 66, 174, 310
AfiI CCNNNNNNNGG 1 cut(s) 148
AgsI TTSAA 2 cut(s) 206, 362
AjiI CACGTC 1 cut(s) 16
AluBI AGCT 1 cut(s) 299
AluI AGCT 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 225
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 3 cut(s) 83, 144, 299
ApoI RAATTY 2 cut(s) 47, 362
AspS9I GGNCC 1 cut(s) 368
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 2 cut(s) 170, 310
AvaI CYCGRG 1 cut(s) 225
AvaII GGWCC 1 cut(s) 368
BalI TGGCCA 1 cut(s) 27
BarI GAAGNNNNNNTAC 2 cut(s) 156, 188
BbsI GAAGAC 1 cut(s) 10
BbvI GCAGC 3 cut(s) 95, 156, 286
BccI CCATC 1 cut(s) 406
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BclI TGATCA 2 cut(s) 73, 315
BcnI CCSGG 1 cut(s) 59
BisI GCNGC 4 cut(s) 84, 142, 145, 300
BlsI GCNGC 4 cut(s) 85, 143, 146, 301
BmcAI AGTACT 1 cut(s) 66
Bme1390I CCNGG 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 368
BmeT110I CYCGRG 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 1 cut(s) 370
BmrFI CCNGG 1 cut(s) 59
BmsI GCATC 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 10
BpuMI CCSGG 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 168, 181
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse1I ACTGG 3 cut(s) 53, 271, 306
BseDI CCNNGG 2 cut(s) 168, 181
BseGI GGATG 1 cut(s) 157
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 1 cut(s) 372
BseNI ACTGG 3 cut(s) 53, 271, 306
BseXI GCAGC 3 cut(s) 95, 156, 286
BshFI GGCC 1 cut(s) 27
BsiHKCI CYCGRG 1 cut(s) 225
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 41
BslI CCNNNNNNNGG 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 41
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 1 cut(s) 27
BsoBI CYCGRG 1 cut(s) 225
Bsp143I GATC 4 cut(s) 73, 190, 315, 404
BspACI CCGC 2 cut(s) 142, 372
BspANI GGCC 1 cut(s) 27
BspCNI CTCAG 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 370
BsrI ACTGG 3 cut(s) 53, 271, 306
BssECI CCNNGG 2 cut(s) 168, 181
BssMI GATC 4 cut(s) 73, 190, 315, 404
BssT1I CCWWGG 2 cut(s) 168, 181
BstDEI CTNAG 1 cut(s) 381
BstF5I GGATG 1 cut(s) 157
BstKTI GATC 4 cut(s) 76, 193, 318, 407
BstMBI GATC 4 cut(s) 73, 190, 315, 404
BstSCI CCNGG 1 cut(s) 57
BstV1I GCAGC 3 cut(s) 95, 156, 286
BstV2I GAAGAC 1 cut(s) 10
BsuRI GGCC 1 cut(s) 27
BtrI CACGTC 1 cut(s) 16
BtsCI GGATG 1 cut(s) 157
BtsIMutI CAGTG 2 cut(s) 69, 264
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 3 cut(s) 65, 173, 309
CviAII CATG 5 cut(s) 77, 93, 313, 427, 435
CviJI RGCY 6 cut(s) 27, 141, 167, 197, 299, 397
CviKI_1 RGCY 6 cut(s) 27, 141, 167, 197, 299, 397
CviQI GTAC 3 cut(s) 65, 173, 309
DdeI CTNAG 1 cut(s) 381
DpnI GATC 4 cut(s) 75, 192, 317, 406
DpnII GATC 4 cut(s) 73, 190, 315, 404
EaeI YGGCCR 1 cut(s) 25
Eco130I CCWWGG 2 cut(s) 168, 181
Eco47I GGWCC 1 cut(s) 368
Eco88I CYCGRG 1 cut(s) 225
EcoRI GAATTC 1 cut(s) 362
EcoT14I CCWWGG 2 cut(s) 168, 181
EcoT22I ATGCAT 1 cut(s) 440
ErhI CCWWGG 2 cut(s) 168, 181
FaeI CATG 5 cut(s) 80, 96, 316, 430, 438
FaiI YATR 7 cut(s) 78, 94, 314, 323, 428, 436, 440
FaqI GGGAC 1 cut(s) 41
FatI CATG 5 cut(s) 76, 92, 312, 426, 434
FauI CCCGC 1 cut(s) 379
FbaI TGATCA 2 cut(s) 73, 315
Fnu4HI GCNGC 4 cut(s) 84, 142, 145, 300
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 4 cut(s) 84, 142, 145, 300
GluI GCNGC 4 cut(s) 84, 142, 145, 300
HaeIII GGCC 1 cut(s) 27
HapII CCGG 1 cut(s) 59
Hin1II CATG 5 cut(s) 80, 96, 316, 430, 438
HinfI GANTC 1 cut(s) 206
HpaII CCGG 1 cut(s) 59
HphI GGTGA 2 cut(s) 170, 310
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 1 cut(s) 368
Hpy188III TCNNGA 3 cut(s) 44, 225, 402
Hpy8I GTNNAC 1 cut(s) 232
HpyAV CCTTC 1 cut(s) 50
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 3 cut(s) 83, 430, 438
HpyF3I CTNAG 1 cut(s) 381
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 5 cut(s) 80, 96, 316, 430, 438
Ksp22I TGATCA 2 cut(s) 73, 315
Kzo9I GATC 4 cut(s) 73, 190, 315, 404
LpnPI CCDG 9 cut(s) 9, 41, 57, 66, 72, 133, 179, 252, 319
Lsp1109I GCAGC 3 cut(s) 95, 156, 286
LweI GCATC 1 cut(s) 142
MaeII ACGT 1 cut(s) 15
MalI GATC 4 cut(s) 75, 192, 317, 406
MboI GATC 4 cut(s) 73, 190, 315, 404
MboII GAAGA 2 cut(s) 10, 200
MlsI TGGCCA 1 cut(s) 27
MluCI AATT 3 cut(s) 47, 158, 362
MluNI TGGCCA 1 cut(s) 27
MnlI CCTC 3 cut(s) 108, 112, 376
Mox20I TGGCCA 1 cut(s) 27
Mph1103I ATGCAT 1 cut(s) 440
MscI TGGCCA 1 cut(s) 27
MslI CAYNNNNRTG 1 cut(s) 269
Msp20I TGGCCA 1 cut(s) 27
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 214
NciI CCSGG 1 cut(s) 59
NdeII GATC 4 cut(s) 73, 190, 315, 404
NlaIII CATG 5 cut(s) 80, 96, 316, 430, 438
NlaIV GGNNCC 1 cut(s) 370
NsiI ATGCAT 1 cut(s) 440
PctI GAATGC 1 cut(s) 214
PfeI GAWTC 1 cut(s) 206
PfoI TCCNGGA 1 cut(s) 57
PkrI GCNGC 4 cut(s) 85, 143, 146, 301
PspN4I GGNNCC 1 cut(s) 370
PspPI GGNCC 1 cut(s) 368
RsaI GTAC 3 cut(s) 66, 174, 310
RsaNI GTAC 3 cut(s) 65, 173, 309
RseI CAYNNNNRTG 1 cut(s) 269
SatI GCNGC 4 cut(s) 84, 142, 145, 300
Sau3AI GATC 4 cut(s) 73, 190, 315, 404
Sau96I GGNCC 1 cut(s) 368
ScaI AGTACT 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 59
SetI ASST 6 cut(s) 18, 42, 174, 183, 301, 343
SfaNI GCATC 1 cut(s) 142
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 269
Sse9I AATT 3 cut(s) 47, 158, 362
SsiI CCGC 2 cut(s) 142, 372
SspI AATATT 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 2 cut(s) 168, 181
TaiI ACGT 1 cut(s) 18
TaqI TCGA 4 cut(s) 104, 291, 345, 393
TasI AATT 3 cut(s) 47, 158, 362
TatI WGTACW 1 cut(s) 64
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 1 cut(s) 206
TscAI CASTG 2 cut(s) 76, 271
TseI GCWGC 3 cut(s) 83, 144, 299
TspDTI ATGAA 1 cut(s) 81
TspRI CASTG 2 cut(s) 76, 271
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 2 cut(s) 47, 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 20
ZrmI AGTACT 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.