Rh6DG338300

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001757
Physical Location & Seq
Forward (+)
54246673 .. 54247077
405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG338300.1

Sequence Viewer

Length: 405 bp
ATGTTCTTCTTCCACGTCTTCTCTGGCCAGAGAATAGACCTTCCTGAATTCCCAGTCCCGGAACTCACTGATCATGTTGCAGCAGTTTCATGTCCTCCCTCGACAACTTCCCAAGATTGCGTGGTTTGTGTCATTAGCCGCAGCAGGGATGCTGAATTGGAAGCCAAGGTACTTCACCTTGGAGAAGATCAGGCTTGGATTGAATCAAAGCATTCTCGTTCCCGAGTTCACACCGATACAATCAAATGTGCTGTTTATCACACTGGAATGGGAATGTTCTGCTTTTTCGATAGAGCTGCTTCCAGTACCATGATCACCATATCTACTGAAAAGCGACCTATCGAATGGAAATGTATTGAATTCGGACCCGCAAAAACTGAGGAAAATGTCGAGCCATCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

134

Amino Acids

15.12

Weight (kDa)

5.64

Isoelectric Point (pI)

63.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 1 - 112 6.3e-07 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 139, 369
AcoI YGGCCR 1 cut(s) 25
AcsI RAATTY 2 cut(s) 47, 359
AfaI GTAC 2 cut(s) 171, 307
AfiI CCNNNNNNNGG 2 cut(s) 58, 145
AgsI TTSAA 2 cut(s) 203, 359
AjiI CACGTC 1 cut(s) 16
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 222
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 3 cut(s) 80, 141, 296
ApoI RAATTY 2 cut(s) 47, 359
AspS9I GGNCC 1 cut(s) 365
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 2 cut(s) 167, 307
AvaI CYCGRG 1 cut(s) 222
AvaII GGWCC 1 cut(s) 365
BalI TGGCCA 1 cut(s) 27
BarI GAAGNNNNNNTAC 2 cut(s) 153, 185
BbsI GAAGAC 1 cut(s) 10
BbvI GCAGC 3 cut(s) 92, 153, 283
BccI CCATC 1 cut(s) 403
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BclI TGATCA 2 cut(s) 70, 312
BcnI CCSGG 1 cut(s) 59
BisI GCNGC 4 cut(s) 81, 139, 142, 297
BlsI GCNGC 4 cut(s) 82, 140, 143, 298
Bme1390I CCNGG 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 365
BmeT110I CYCGRG 1 cut(s) 222
BmgBI CACGTC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 1 cut(s) 367
BmrFI CCNGG 1 cut(s) 59
BmrI ACTGGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 139
BmuI ACTGGG 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 10
BpuMI CCSGG 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 165, 178
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 145
Bse1I ACTGG 3 cut(s) 53, 268, 303
BseDI CCNNGG 2 cut(s) 165, 178
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 2 cut(s) 58, 145
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 3 cut(s) 53, 268, 303
BseXI GCAGC 3 cut(s) 92, 153, 283
BshFI GGCC 1 cut(s) 27
BsiHKCI CYCGRG 1 cut(s) 222
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 41
BslI CCNNNNNNNGG 2 cut(s) 58, 145
BsmFI GGGAC 1 cut(s) 41
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 1 cut(s) 27
BsoBI CYCGRG 1 cut(s) 222
Bsp143I GATC 3 cut(s) 70, 187, 312
BspACI CCGC 2 cut(s) 139, 369
BspANI GGCC 1 cut(s) 27
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 367
BsrI ACTGG 3 cut(s) 53, 268, 303
BssECI CCNNGG 2 cut(s) 165, 178
BssMI GATC 3 cut(s) 70, 187, 312
BssT1I CCWWGG 2 cut(s) 165, 178
BstDEI CTNAG 1 cut(s) 378
BstF5I GGATG 1 cut(s) 154
BstKTI GATC 3 cut(s) 73, 190, 315
BstMBI GATC 3 cut(s) 70, 187, 312
BstSCI CCNGG 1 cut(s) 57
BstV1I GCAGC 3 cut(s) 92, 153, 283
BstV2I GAAGAC 1 cut(s) 10
BsuRI GGCC 1 cut(s) 27
BtrI CACGTC 1 cut(s) 16
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 2 cut(s) 66, 261
Cfr13I GGNCC 1 cut(s) 365
Csp6I GTAC 2 cut(s) 170, 306
CviAII CATG 3 cut(s) 74, 90, 310
CviJI RGCY 6 cut(s) 27, 138, 164, 194, 296, 394
CviKI_1 RGCY 6 cut(s) 27, 138, 164, 194, 296, 394
CviQI GTAC 2 cut(s) 170, 306
DdeI CTNAG 1 cut(s) 378
DpnI GATC 3 cut(s) 72, 189, 314
DpnII GATC 3 cut(s) 70, 187, 312
EaeI YGGCCR 1 cut(s) 25
Eco130I CCWWGG 2 cut(s) 165, 178
Eco47I GGWCC 1 cut(s) 365
Eco88I CYCGRG 1 cut(s) 222
EcoRI GAATTC 2 cut(s) 47, 359
EcoT14I CCWWGG 2 cut(s) 165, 178
ErhI CCWWGG 2 cut(s) 165, 178
FaeI CATG 3 cut(s) 77, 93, 313
FaiI YATR 4 cut(s) 75, 91, 311, 320
FaqI GGGAC 1 cut(s) 41
FatI CATG 3 cut(s) 73, 89, 309
FauI CCCGC 1 cut(s) 376
FbaI TGATCA 2 cut(s) 70, 312
Fnu4HI GCNGC 4 cut(s) 81, 139, 142, 297
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 4 cut(s) 81, 139, 142, 297
GluI GCNGC 4 cut(s) 81, 139, 142, 297
HaeIII GGCC 1 cut(s) 27
HapII CCGG 1 cut(s) 59
Hin1II CATG 3 cut(s) 77, 93, 313
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 59
HphI GGTGA 2 cut(s) 167, 307
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 365
Hpy188III TCNNGA 3 cut(s) 44, 222, 399
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 1 cut(s) 50
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 1 cut(s) 80
HpyF3I CTNAG 1 cut(s) 378
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 3 cut(s) 77, 93, 313
Ksp22I TGATCA 2 cut(s) 70, 312
Kzo9I GATC 3 cut(s) 70, 187, 312
LpnPI CCDG 9 cut(s) 9, 41, 57, 66, 72, 130, 176, 249, 316
Lsp1109I GCAGC 3 cut(s) 92, 153, 283
LweI GCATC 1 cut(s) 139
MaeII ACGT 1 cut(s) 15
MalI GATC 3 cut(s) 72, 189, 314
MboI GATC 3 cut(s) 70, 187, 312
MboII GAAGA 2 cut(s) 10, 197
MlsI TGGCCA 1 cut(s) 27
MluCI AATT 3 cut(s) 47, 155, 359
MluNI TGGCCA 1 cut(s) 27
MnlI CCTC 3 cut(s) 105, 109, 373
Mox20I TGGCCA 1 cut(s) 27
MscI TGGCCA 1 cut(s) 27
MslI CAYNNNNRTG 1 cut(s) 266
Msp20I TGGCCA 1 cut(s) 27
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 211
NciI CCSGG 1 cut(s) 59
NdeII GATC 3 cut(s) 70, 187, 312
NlaIII CATG 3 cut(s) 77, 93, 313
NlaIV GGNNCC 1 cut(s) 367
PctI GAATGC 1 cut(s) 211
PfeI GAWTC 1 cut(s) 203
PfoI TCCNGGA 1 cut(s) 57
PkrI GCNGC 4 cut(s) 82, 140, 143, 298
PspN4I GGNNCC 1 cut(s) 367
PspPI GGNCC 1 cut(s) 365
RsaI GTAC 2 cut(s) 171, 307
RsaNI GTAC 2 cut(s) 170, 306
RseI CAYNNNNRTG 1 cut(s) 266
SatI GCNGC 4 cut(s) 81, 139, 142, 297
Sau3AI GATC 3 cut(s) 70, 187, 312
Sau96I GGNCC 1 cut(s) 365
ScrFI CCNGG 1 cut(s) 59
SetI ASST 6 cut(s) 18, 42, 171, 180, 298, 340
SfaNI GCATC 1 cut(s) 139
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 266
Sse9I AATT 3 cut(s) 47, 155, 359
SsiI CCGC 2 cut(s) 139, 369
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 2 cut(s) 165, 178
TaiI ACGT 1 cut(s) 18
TaqI TCGA 4 cut(s) 101, 288, 342, 390
TasI AATT 3 cut(s) 47, 155, 359
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 1 cut(s) 203
TscAI CASTG 2 cut(s) 73, 268
TseI GCWGC 3 cut(s) 80, 141, 296
TspDTI ATGAA 1 cut(s) 78
TspRI CASTG 2 cut(s) 73, 268
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 2 cut(s) 47, 359
XcmI CCANNNNNNNNNTGG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.