Rorug06G0227500

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001757
Physical Location & Seq
Forward (+)
36534343 .. 36536155
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0227500.1

Sequence Viewer

Length: 867 bp
ATGCTTGGTGGGGATTGGTCGAGAAAGACAGGACCTTCCAAGTACAACTGGGAAGGGTATGCAGAGCTTGTGAACTTGGTCCAAAAGCATGGCTTGAAGATTCAAGTTGTCATGTCTTTCCATCAGTGTGGAGGAAATGTTGGAGACTCATGCTGTATTCCACTACCTCCATGGGTGCTTGAAGAGATAAGCAAGAACCCTGACCTTGTGTACACAGACAAATCTGGCAGGAGGAATCCTGAGTACATATCCTTGGGCTGTGATTCATTGCCTGTTCTCGGAGGAAGAATGCCCATCCAGGTTTACACCGACTACATGAAGAGCTTCCATGAGAGATTCAGAGATTACTTGGGAGACGTTATTGTGGAAATTCAAGTTGGTATGGGTCCTTGTGGTGAACTCAGATATCCTGCTTATCCAGAGAGCAATGGAACATGGAAGTTTCCTGGAATTGGAGAATTTCAATGCTATGACAAGTACATGAAAGCTTCCCTGGAAGCATTAGCAGAGGCACAAGGAAAGAGAGATTGGGGAAGGACTGGACCCCATGATTCCGGCCAGTACAATCAGTTTCCTGAAGACACCGGATTTTTCAAAAGAGATGGAACCTGGAATACCGAGTATGGACAGTTCTTCCTCGAATGGTACTCAGAGAAGCTATTAAGGCACGGAGATAGACTCCTGGCAGCTGCAAAAGGAGTGTTTCAAGGAACTGGAGCTAAACTATCTGGAAAGGTAGCTGGGATTCATTGGCACTACAGAACAAGATCCCATGCTGCTGAATTAATGCTGGCTACTATAATACTAGACTTGGAGATGGTTACATACCAACAGCAAAAATGTTCAGCAAACATGGGGTTGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

288

Amino Acids

32.61

Weight (kDa)

6.45

Isoelectric Point (pI)

30.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 8 - 262 1.8e-129 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 762
AcoI YGGCCR 1 cut(s) 556
AcsI RAATTY 2 cut(s) 369, 458
AcuI CTGAAG 1 cut(s) 597
AfaI GTAC 6 cut(s) 44, 212, 245, 479, 563, 647
AfiI CCNNNNNNNGG 2 cut(s) 278, 452
AgsI TTSAA 7 cut(s) 97, 104, 182, 374, 464, 595, 707
AjnI CCWGG 5 cut(s) 297, 445, 492, 608, 681
AjuI GAANNNNNNNTTGG 4 cut(s) 360, 392, 511, 543
AluBI AGCT 7 cut(s) 67, 324, 488, 658, 689, 719, 740
AluI AGCT 7 cut(s) 67, 324, 488, 658, 689, 719, 740
Alw26I GTCTC 2 cut(s) 138, 348
AlwI GGATC 1 cut(s) 762
AoxI GGCC 1 cut(s) 556
ApeKI GCWGC 3 cut(s) 686, 689, 776
ApoI RAATTY 2 cut(s) 369, 458
AseI ATTAAT 1 cut(s) 785
Asp700I GAANNNNTTC 1 cut(s) 323
AspS9I GGNCC 4 cut(s) 32, 79, 386, 542
AsuHPI GGTGA 1 cut(s) 407
AvaII GGWCC 4 cut(s) 32, 79, 386, 542
BbsI GAAGAC 1 cut(s) 585
BbvI GCAGC 3 cut(s) 676, 698, 763
BccI CCATC 4 cut(s) 129, 302, 596, 811
BciT130I CCWGG 5 cut(s) 299, 447, 494, 610, 683
BcoDI GTCTC 2 cut(s) 138, 348
BfaI CTAG 1 cut(s) 806
BfmI CTRYAG 1 cut(s) 757
BisI GCNGC 3 cut(s) 687, 690, 777
BlsI GCNGC 3 cut(s) 688, 691, 778
Bme1390I CCNGG 5 cut(s) 299, 447, 494, 610, 683
Bme18I GGWCC 4 cut(s) 32, 79, 386, 542
BmgT120I GGNCC 4 cut(s) 32, 79, 386, 542
BmiI GGNNCC 3 cut(s) 387, 544, 607
BmrFI CCNGG 5 cut(s) 299, 447, 494, 610, 683
BmrI ACTGGG 1 cut(s) 58
BmuI ACTGGG 1 cut(s) 58
BpiI GAAGAC 1 cut(s) 585
BplI GAGNNNNNCTC 2 cut(s) 663, 695
BpmI CTGGAG 1 cut(s) 735
BsaJI CCNNGG 3 cut(s) 170, 252, 492
BsaWI WCCGGW 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 452
Bse1I ACTGG 4 cut(s) 53, 544, 559, 718
Bse3DI GCAATG 2 cut(s) 266, 433
BseBI CCWGG 5 cut(s) 299, 447, 494, 610, 683
BseDI CCNNGG 3 cut(s) 170, 252, 492
BseGI GGATG 1 cut(s) 294
BseLI CCNNNNNNNGG 2 cut(s) 278, 452
BseMI GCAATG 2 cut(s) 266, 433
BseMII CTCAG 3 cut(s) 231, 415, 663
BseNI ACTGG 4 cut(s) 53, 544, 559, 718
BseXI GCAGC 3 cut(s) 676, 698, 763
BseYI CCCAGC 1 cut(s) 740
BshFI GGCC 1 cut(s) 558
BsiSI CCGG 2 cut(s) 555, 585
BslI CCNNNNNNNGG 2 cut(s) 278, 452
BsmAI GTCTC 2 cut(s) 138, 348
BsmBI CGTCTC 1 cut(s) 348
BsmI GAATGC 1 cut(s) 294
BsnI GGCC 1 cut(s) 558
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 1 cut(s) 767
Bsp19I CCATGG 1 cut(s) 170
BspANI GGCC 1 cut(s) 558
BspCNI CTCAG 3 cut(s) 232, 414, 662
BspLI GGNNCC 3 cut(s) 387, 544, 607
BspPI GGATC 1 cut(s) 762
BspQI GCTCTTC 1 cut(s) 314
BsrDI GCAATG 2 cut(s) 266, 433
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 4 cut(s) 53, 544, 559, 718
BssECI CCNNGG 3 cut(s) 170, 252, 492
BssMI GATC 1 cut(s) 767
BssT1I CCWWGG 2 cut(s) 170, 252
Bst2UI CCWGG 5 cut(s) 299, 447, 494, 610, 683
Bst4CI ACNGT 1 cut(s) 630
Bst6I CTCTTC 2 cut(s) 177, 314
BstAUI TGTACA 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 792
BstDEI CTNAG 3 cut(s) 240, 401, 649
BstDSI CCRYGG 1 cut(s) 170
BstF5I GGATG 1 cut(s) 294
BstKTI GATC 1 cut(s) 770
BstMAI GTCTC 2 cut(s) 138, 348
BstMBI GATC 1 cut(s) 767
BstMWI GCNNNNNNNGC 1 cut(s) 664
BstNI CCWGG 5 cut(s) 299, 447, 494, 610, 683
BstSCI CCNGG 5 cut(s) 297, 445, 492, 608, 681
BstSFI CTRYAG 1 cut(s) 757
BstV1I GCAGC 3 cut(s) 676, 698, 763
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 1 cut(s) 767
BstXI CCANNNNNNTGG 2 cut(s) 89, 128
BstYI RGATCY 1 cut(s) 767
BsuRI GGCC 1 cut(s) 558
BtgI CCRYGG 1 cut(s) 170
BtsCI GGATG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 131
Cac8I GCNNGC 1 cut(s) 792
Cfr13I GGNCC 4 cut(s) 32, 79, 386, 542
Csp6I GTAC 6 cut(s) 43, 211, 244, 478, 562, 646
CviQI GTAC 6 cut(s) 43, 211, 244, 478, 562, 646
DdeI CTNAG 3 cut(s) 240, 401, 649
DpnI GATC 1 cut(s) 769
DpnII GATC 1 cut(s) 767
EaeI YGGCCR 1 cut(s) 556
Eam1104I CTCTTC 2 cut(s) 177, 314
EarI CTCTTC 2 cut(s) 177, 314
Eco130I CCWWGG 2 cut(s) 170, 252
Eco32I GATATC 1 cut(s) 407
Eco47I GGWCC 4 cut(s) 32, 79, 386, 542
Eco57I CTGAAG 1 cut(s) 597
EcoO109I RGGNCCY 2 cut(s) 32, 386
EcoRII CCWGG 5 cut(s) 297, 445, 492, 608, 681
EcoRV GATATC 1 cut(s) 407
EcoT14I CCWWGG 2 cut(s) 170, 252
ErhI CCWWGG 2 cut(s) 170, 252
Esp3I CGTCTC 1 cut(s) 348
FalI AAGNNNNNCTT 2 cut(s) 77, 109
Fnu4HI GCNGC 3 cut(s) 687, 690, 777
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 3 cut(s) 687, 690, 777
FspBI CTAG 1 cut(s) 806
GluI GCNGC 3 cut(s) 687, 690, 777
GsaI CCCAGC 1 cut(s) 744
GsuI CTGGAG 1 cut(s) 735
HaeIII GGCC 1 cut(s) 558
HapII CCGG 2 cut(s) 555, 585
HindIII AAGCTT 1 cut(s) 486
HinfI GANTC 8 cut(s) 100, 146, 235, 263, 336, 551, 678, 745
HpaII CCGG 2 cut(s) 555, 585
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 5 cut(s) 73, 211, 213, 304, 398
Hpy188I TCNGA 4 cut(s) 281, 341, 404, 652
Hpy188III TCNNGA 5 cut(s) 21, 239, 419, 575, 729
Hpy8I GTNNAC 5 cut(s) 73, 211, 213, 304, 398
HpyAV CCTTC 3 cut(s) 45, 47, 528
HpyCH4III ACNGT 1 cut(s) 630
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 2 cut(s) 62, 692
HpyF10VI GCNNNNNNNGC 1 cut(s) 664
HpyF3I CTNAG 3 cut(s) 240, 401, 649
HpySE526I ACGT 1 cut(s) 357
Kzo9I GATC 1 cut(s) 767
LguI GCTCTTC 1 cut(s) 314
LmnI GCTCC 1 cut(s) 716
Lsp1109I GCAGC 3 cut(s) 676, 698, 763
MaeI CTAG 1 cut(s) 806
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 1 cut(s) 820
MalI GATC 1 cut(s) 769
MboI GATC 1 cut(s) 767
MboII GAAGA 6 cut(s) 109, 194, 297, 331, 590, 625
MflI RGATCY 1 cut(s) 767
MluCI AATT 4 cut(s) 369, 450, 458, 782
MlyI GAGTC 2 cut(s) 140, 672
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 6 cut(s) 125, 177, 225, 275, 502, 647
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 3 cut(s) 662, 785, 865
MslI CAYNNNNRTG 1 cut(s) 126
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 2 cut(s) 555, 585
MspR9I CCNGG 5 cut(s) 299, 447, 494, 610, 683
Mva1269I GAATGC 1 cut(s) 294
MvaI CCWGG 5 cut(s) 299, 447, 494, 610, 683
MwoI GCNNNNNNNGC 1 cut(s) 664
NcoI CCATGG 1 cut(s) 170
NdeII GATC 1 cut(s) 767
NlaIV GGNNCC 3 cut(s) 387, 544, 607
PciSI GCTCTTC 1 cut(s) 314
PctI GAATGC 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 6 cut(s) 100, 235, 263, 336, 551, 745
PfoI TCCNGGA 1 cut(s) 445
PkrI GCNGC 3 cut(s) 688, 691, 778
PleI GAGTC 2 cut(s) 140, 672
PpsI GAGTC 2 cut(s) 140, 672
PpuMI RGGWCCY 2 cut(s) 32, 386
PshBI ATTAAT 1 cut(s) 785
Psp5II RGGWCCY 2 cut(s) 32, 386
Psp6I CCWGG 5 cut(s) 297, 445, 492, 608, 681
PspFI CCCAGC 1 cut(s) 740
PspGI CCWGG 5 cut(s) 297, 445, 492, 608, 681
PspN4I GGNNCC 3 cut(s) 387, 544, 607
PspPI GGNCC 4 cut(s) 32, 79, 386, 542
PspPPI RGGWCCY 2 cut(s) 32, 386
PsrI GAACNNNNNNTAC 4 cut(s) 598, 614, 630, 646
PsuI RGATCY 1 cut(s) 767
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 6 cut(s) 44, 212, 245, 479, 563, 647
RsaNI GTAC 6 cut(s) 43, 211, 244, 478, 562, 646
RseI CAYNNNNRTG 1 cut(s) 126
SapI GCTCTTC 1 cut(s) 314
SaqAI TTAA 3 cut(s) 662, 785, 865
SatI GCNGC 3 cut(s) 687, 690, 777
Sau3AI GATC 1 cut(s) 767
Sau96I GGNCC 4 cut(s) 32, 79, 386, 542
SchI GAGTC 2 cut(s) 140, 672
ScrFI CCNGG 5 cut(s) 299, 447, 494, 610, 683
SfcI CTRYAG 1 cut(s) 757
SinI GGWCC 4 cut(s) 32, 79, 386, 542
SmiMI CAYNNNNRTG 1 cut(s) 126
Sse9I AATT 4 cut(s) 369, 450, 458, 782
SspMI CTAG 1 cut(s) 806
StyD4I CCNGG 5 cut(s) 297, 445, 492, 608, 681
StyI CCWWGG 2 cut(s) 170, 252
TaaI ACNGT 1 cut(s) 630
TaiI ACGT 1 cut(s) 360
TaqI TCGA 2 cut(s) 20, 639
TasI AATT 4 cut(s) 369, 450, 458, 782
TatI WGTACW 5 cut(s) 42, 210, 243, 477, 561
TfiI GAWTC 6 cut(s) 100, 235, 263, 336, 551, 745
Tru1I TTAA 3 cut(s) 662, 785, 865
Tru9I TTAA 3 cut(s) 662, 785, 865
TscAI CASTG 1 cut(s) 131
TseI GCWGC 3 cut(s) 686, 689, 776
TspDTI ATGAA 4 cut(s) 255, 332, 497, 737
TspGWI ACGGA 1 cut(s) 684
TspRI CASTG 1 cut(s) 131
VpaK11BI GGWCC 4 cut(s) 32, 79, 386, 542
VspI ATTAAT 1 cut(s) 785
XapI RAATTY 2 cut(s) 369, 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.