FvH4_2g24390

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001757
Physical Location & Seq
Forward (+)
19923424 .. 19926083
2660 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24390.t1

Sequence Viewer

Length: 1092 bp
ATGAAGTTCTGTCTCTCCTCCAAAAAAAAGTTCCTTTGCATGGAGTGTTCGAGGTGGCTCTACGACACAATCTTAGAGCCAGCATGCTTATATGTGACGAAGAAATACAAAATCATCACTACGGGGCAAAGGCAAAATAGGGCATGGTCTGATCTCCCTCCTGAGATTCTTTCACTCATAGCAGAGCGTGCAGGCCTATTCGAGCTTCTGAGTTTCTTTCGTGTCTGCAACAGTTGGCATTCTGCTTCTTCCACCGCCTTAGCTCACATTGTTGGGCATGAAATAAACAAAGGACCTTGGTTTGTACTATACGAGGATAAAAACTCCGAGTGTCAGTTGCTAACTTCAAGTGGTAAGAAGTTCACCACCAGTCTCCCTGAACTGGATGGAACAACTTGCCTTGCAACGTACGGGGCATGGTTTCTGTTATTCAAGAAAGGAGGTTCCATGTTCTTCTTTCACGTCCTCTTGCGCCAGAGAATAGACCTTCCAAAATTCCCAGTTCCAGAACTCACTGATCATGTTGGAGCAGTTTCCTCGTCTCCAACTTCCCAAGATTGTGTGGTTTGTGTCATTAGCTGTAGAGATGATAAGACATTGGATGCTAAGCTACATTTCCTTGGAAAGAAGACTTACTGGATTGTGGCAACGCATTATGATGCTCGAGTTAAAACAGACACAATCAAATCTGCAGTCTATCACAATGGAAAATTCTGCATTTTTGATGGTAGACTGGATAATATGAGCATGATGACCCTATCTATTGAAAACAGTTTTCCCTACTGCATCTGGAAATATGTAACACTAGTAAAAGCAAACCCTCGGAATAGTCACAAGGACAACCCCTCAAAATCAAACGTGTTTCAACGTCACGTGGATGAGTTGAAGAATAATCTGAAGGACATGAAGAAGAAGCTGGGTTTGACAGAAGATGTTTCAATTTCTACTTGTGGGACAATTACACCCTTAATAGAAGAAGATAAGAGCAAGTTTATTTTTAATGAGAGCGTCAGCGACAATGATGACAAATCCAAGAACCGTCGTTTCAAAGGGGTATGGATCCAACCAAGATCCTCAGCTAGGAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

364

Amino Acids

41.58

Weight (kDa)

9.14

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 118 - 265 4.8e-14 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 730
AciI CCGC 1 cut(s) 255
AclWI GGATC 3 cut(s) 1054, 1065, 1067
AcsI RAATTY 2 cut(s) 494, 710
AcuI CTGAAG 1 cut(s) 917
AcvI CACGTG 1 cut(s) 874
AfaI GTAC 2 cut(s) 306, 410
AfiI CCNNNNNNNGG 3 cut(s) 40, 382, 1080
AflIII ACRYGT 1 cut(s) 858
AgsI TTSAA 7 cut(s) 348, 433, 767, 866, 886, 939, 1048
AhlI ACTAGT 1 cut(s) 805
AjiI CACGTC 1 cut(s) 463
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 6 cut(s) 205, 263, 579, 610, 916, 1079
AluI AGCT 6 cut(s) 205, 263, 579, 610, 916, 1079
Alw26I GTCTC 3 cut(s) 17, 377, 546
AlwI GGATC 3 cut(s) 1054, 1065, 1067
Ama87I CYCGRG 1 cut(s) 663
AoxI GGCC 1 cut(s) 193
ApoI RAATTY 2 cut(s) 494, 710
ArsI GACNNNNNNTTYG 2 cut(s) 678, 710
AspLEI GCGC 1 cut(s) 474
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 355
AvaI CYCGRG 1 cut(s) 663
AvaII GGWCC 1 cut(s) 293
BamHI GGATCC 1 cut(s) 1059
BbrPI CACGTG 1 cut(s) 874
BbsI GAAGAC 1 cut(s) 635
BbvCI CCTCAGC 1 cut(s) 1075
BccI CCATC 3 cut(s) 380, 719, 1080
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 3 cut(s) 17, 377, 546
BcuI ACTAGT 1 cut(s) 805
BfaI CTAG 2 cut(s) 806, 1080
BfmI CTRYAG 2 cut(s) 580, 690
BlpI GCTNAGC 1 cut(s) 606
Bme18I GGWCC 1 cut(s) 293
BmeT110I CYCGRG 1 cut(s) 663
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 445, 1061
BmrI ACTGGG 1 cut(s) 494
BmsI GCATC 3 cut(s) 592, 649, 795
BmuI ACTGGG 1 cut(s) 494
BpiI GAAGAC 1 cut(s) 635
Bpu10I CCTNAGC 2 cut(s) 259, 1075
Bpu1102I GCTNAGC 1 cut(s) 606
BsaAI YACGTR 1 cut(s) 874
BsaJI CCNNGG 3 cut(s) 296, 619, 821
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 382, 1080
Bse1I ACTGG 5 cut(s) 369, 387, 500, 641, 738
BseDI CCNNGG 3 cut(s) 296, 619, 821
BseGI GGATG 3 cut(s) 391, 607, 883
BseLI CCNNNNNNNGG 3 cut(s) 40, 382, 1080
BseMII CTCAG 3 cut(s) 153, 200, 1089
BseNI ACTGG 5 cut(s) 369, 387, 500, 641, 738
BseRI GAGGAG 1 cut(s) 7
BseYI CCCAGC 1 cut(s) 916
BsgI GTGCAG 1 cut(s) 210
BshFI GGCC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 663
BsiWI CGTACG 1 cut(s) 408
BslFI GGGAC 1 cut(s) 967
BslI CCNNNNNNNGG 3 cut(s) 40, 382, 1080
BsmAI GTCTC 3 cut(s) 17, 377, 546
BsmBI CGTCTC 1 cut(s) 546
BsmFI GGGAC 1 cut(s) 967
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 663
Bsp143I GATC 4 cut(s) 151, 517, 1059, 1070
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 1 cut(s) 255
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 3 cut(s) 154, 201, 1088
BspLI GGNNCC 2 cut(s) 445, 1061
BspMAI CTGCAG 1 cut(s) 694
BspPI GGATC 3 cut(s) 1054, 1065, 1067
BsrI ACTGG 5 cut(s) 369, 387, 500, 641, 738
BssECI CCNNGG 3 cut(s) 296, 619, 821
BssMI GATC 4 cut(s) 151, 517, 1059, 1070
BssT1I CCWWGG 2 cut(s) 296, 619
Bst4CI ACNGT 3 cut(s) 233, 773, 1040
BstAPI GCANNNNNTGC 1 cut(s) 188
BstBAI YACGTR 1 cut(s) 874
BstC8I GCNNGC 4 cut(s) 81, 85, 189, 193
BstDEI CTNAG 6 cut(s) 73, 162, 209, 259, 606, 1075
BstENI CCTNNNNNAGG 1 cut(s) 1078
BstF5I GGATG 3 cut(s) 391, 607, 883
BstHHI GCGC 1 cut(s) 474
BstKTI GATC 4 cut(s) 154, 520, 1062, 1073
BstMAI GTCTC 3 cut(s) 17, 377, 546
BstMBI GATC 4 cut(s) 151, 517, 1059, 1070
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNSI RCATGY 1 cut(s) 87
BstSFI CTRYAG 2 cut(s) 580, 690
BstV2I GAAGAC 1 cut(s) 635
BstX2I RGATCY 2 cut(s) 1059, 1070
BstYI RGATCY 2 cut(s) 1059, 1070
BsuRI GGCC 1 cut(s) 195
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 3 cut(s) 391, 607, 883
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 4 cut(s) 81, 85, 189, 193
CfoI GCGC 1 cut(s) 474
Cfr13I GGNCC 1 cut(s) 293
CseI GACGC 1 cut(s) 997
Csp6I GTAC 2 cut(s) 305, 409
CviAII CATG 9 cut(s) 40, 84, 144, 278, 417, 448, 521, 748, 904
CviJI RGCY 9 cut(s) 58, 79, 195, 205, 263, 579, 610, 916, 1079
CviKI_1 RGCY 9 cut(s) 58, 79, 195, 205, 263, 579, 610, 916, 1079
CviQI GTAC 2 cut(s) 305, 409
DdeI CTNAG 6 cut(s) 73, 162, 209, 259, 606, 1075
DpnI GATC 4 cut(s) 153, 519, 1061, 1072
DpnII GATC 4 cut(s) 151, 517, 1059, 1070
Eco130I CCWWGG 2 cut(s) 296, 619
Eco147I AGGCCT 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 917
Eco72I CACGTG 1 cut(s) 874
Eco88I CYCGRG 1 cut(s) 663
EcoNI CCTNNNNNAGG 1 cut(s) 1078
EcoO109I RGGNCCY 1 cut(s) 293
EcoT14I CCWWGG 2 cut(s) 296, 619
ErhI CCWWGG 2 cut(s) 296, 619
Esp3I CGTCTC 1 cut(s) 546
FaeI CATG 9 cut(s) 43, 87, 147, 281, 420, 451, 524, 751, 907
FaqI GGGAC 1 cut(s) 967
FatI CATG 9 cut(s) 39, 83, 143, 277, 416, 447, 520, 747, 903
FbaI TGATCA 1 cut(s) 517
FblI GTMKAC 1 cut(s) 730
FokI GGATG 3 cut(s) 398, 614, 890
FspBI CTAG 2 cut(s) 806, 1080
GlaI GCGC 1 cut(s) 473
GsaI CCCAGC 1 cut(s) 920
HaeIII GGCC 1 cut(s) 195
HgaI GACGC 1 cut(s) 997
HhaI GCGC 1 cut(s) 474
Hin1II CATG 9 cut(s) 43, 87, 147, 281, 420, 451, 524, 751, 907
Hin6I GCGC 1 cut(s) 472
HinP1I GCGC 1 cut(s) 472
HinfI GANTC 1 cut(s) 166
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 2 cut(s) 363, 731
Hpy188I TCNGA 5 cut(s) 151, 210, 328, 825, 897
Hpy188III TCNNGA 4 cut(s) 161, 433, 506, 790
Hpy8I GTNNAC 2 cut(s) 363, 731
Hpy99I CGWCG 1 cut(s) 1044
HpyAV CCTTC 2 cut(s) 497, 892
HpyCH4III ACNGT 3 cut(s) 233, 773, 1040
HpyCH4IV ACGT 5 cut(s) 407, 462, 858, 868, 873
HpyCH4V TGCA 7 cut(s) 39, 191, 228, 404, 692, 717, 786
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 6 cut(s) 73, 162, 209, 259, 606, 1075
HpySE526I ACGT 5 cut(s) 407, 462, 858, 868, 873
Hsp92II CATG 9 cut(s) 43, 87, 147, 281, 420, 451, 524, 751, 907
HspAI GCGC 1 cut(s) 472
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 4 cut(s) 151, 517, 1059, 1070
LmnI GCTCC 1 cut(s) 527
LweI GCATC 3 cut(s) 592, 649, 795
MaeI CTAG 2 cut(s) 806, 1080
MaeII ACGT 5 cut(s) 407, 462, 858, 868, 873
MaeIII GTNAC 4 cut(s) 94, 799, 830, 869
MalI GATC 4 cut(s) 153, 519, 1061, 1072
MboI GATC 4 cut(s) 151, 517, 1059, 1070
MflI RGATCY 2 cut(s) 1059, 1070
MluCI AATT 4 cut(s) 494, 710, 939, 957
MmeI TCCRAC 3 cut(s) 505, 569, 1087
MseI TTAA 3 cut(s) 669, 968, 999
MslI CAYNNNNRTG 2 cut(s) 657, 876
Mva1269I GAATGC 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 188
NdeII GATC 4 cut(s) 151, 517, 1059, 1070
NlaIII CATG 9 cut(s) 43, 87, 147, 281, 420, 451, 524, 751, 907
NlaIV GGNNCC 2 cut(s) 445, 1061
NmuCI GTSAC 3 cut(s) 94, 830, 869
NspI RCATGY 1 cut(s) 87
PaeI GCATGC 1 cut(s) 87
PaeR7I CTCGAG 1 cut(s) 663
PceI AGGCCT 1 cut(s) 195
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 166
Pfl23II CGTACG 1 cut(s) 408
PmaCI CACGTG 1 cut(s) 874
PmlI CACGTG 1 cut(s) 874
Ppu21I YACGTR 1 cut(s) 874
PpuMI RGGWCCY 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 293
PspCI CACGTG 1 cut(s) 874
PspFI CCCAGC 1 cut(s) 916
PspLI CGTACG 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 445, 1061
PspPI GGNCC 1 cut(s) 293
PspPPI RGGWCCY 1 cut(s) 293
PspXI VCTCGAGB 1 cut(s) 663
PstI CTGCAG 1 cut(s) 694
PsuI RGATCY 2 cut(s) 1059, 1070
RsaI GTAC 2 cut(s) 306, 410
RsaNI GTAC 2 cut(s) 305, 409
RseI CAYNNNNRTG 2 cut(s) 657, 876
SaqAI TTAA 3 cut(s) 669, 968, 999
Sau3AI GATC 4 cut(s) 151, 517, 1059, 1070
Sau96I GGNCC 1 cut(s) 293
SfaNI GCATC 3 cut(s) 592, 649, 795
SfcI CTRYAG 2 cut(s) 580, 690
Sfr274I CTCGAG 1 cut(s) 663
SinI GGWCC 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 663
SmiMI CAYNNNNRTG 2 cut(s) 657, 876
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
SpeI ACTAGT 1 cut(s) 805
SphI GCATGC 1 cut(s) 87
Sse9I AATT 4 cut(s) 494, 710, 939, 957
SseBI AGGCCT 1 cut(s) 195
SsiI CCGC 1 cut(s) 255
SspMI CTAG 2 cut(s) 806, 1080
StuI AGGCCT 1 cut(s) 195
StyI CCWWGG 2 cut(s) 296, 619
TaaI ACNGT 3 cut(s) 233, 773, 1040
TaiI ACGT 5 cut(s) 410, 465, 861, 871, 876
TaqI TCGA 3 cut(s) 50, 201, 664
TasI AATT 4 cut(s) 494, 710, 939, 957
TatI WGTACW 1 cut(s) 304
TfiI GAWTC 1 cut(s) 166
Tru1I TTAA 3 cut(s) 669, 968, 999
Tru9I TTAA 3 cut(s) 669, 968, 999
TscAI CASTG 1 cut(s) 520
TseFI GTSAC 3 cut(s) 94, 830, 869
Tsp45I GTSAC 3 cut(s) 94, 830, 869
TspDTI ATGAA 3 cut(s) 17, 294, 920
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 293
XagI CCTNNNNNAGG 1 cut(s) 1078
XapI RAATTY 2 cut(s) 494, 710
XceI RCATGY 1 cut(s) 87
XhoI CTCGAG 1 cut(s) 663
XmiI GTMKAC 1 cut(s) 730
XspI CTAG 2 cut(s) 806, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.