FvH4_2g24391

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001757
Physical Location & Seq
Forward (+)
19926474 .. 19928473
2000 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24391.t1

Sequence Viewer

Length: 1059 bp
ATGGAGGGTTTGAAGTGGCTCTACGACACAATCTTAAAGCCAGCAGGCTTATATGTGACGAAGAAAAACAAAATCATCACTACGGAGCAAAGGGAACATAGGCATAGGGCATGGTCTGATCTCCCTCCTGAGCTTCTTTCTCTCATAGCAGAACGTGCAGGCCTATTGGAGCTTCTGAGTTTCTATCGTGTCTGCAACCCTTGGCGCTGCGCTTCTTCTACCGCTTTAGCTCACATAGTTGAGCATGAACTAAACAAAGGACCTTGGTTTCTACTCTACGAGGATAAAAATTCCGAGTGTCAGTTGCTAACTACAAGTGGTAAGAAATTCACTGTGAGTCTCCCGGAACTGGATGGAACCACTTGCCTTGCAACATACAATGCATGGCTTCTTTTATTCAAACAAGGAGGTTCCATGTTCTTCTTTCACATCTTCTCTCGCCGGAGAATAGACCTTCCAAAATTCCCATTCTCGGACCTCACCGATCATGTTGCATCAGTTTCATCGTCTCCAACTTCCCAAGATTGCGTGGTTTGTGTCATCAGCCGCAGCAGTGATGAAGCCTTGGATGTCAAGGCACTGTTATTGGGAGACCGGGTTTGGTTAAGCTTAAAAGTTAATCATGCCCGAGTTCAAACAGATACAATCAAGTGTGGTGTCTATCATTCTGGAATATTTATCTTCTTTGATGATGTACATGATAACTTGAATATGCCCACCATATTCATTGAAAATCGATCTATCTGCTATATGTGTGAGCAAATAGAATTCGTAGCCAACGAGGAAATTGATCAGAAGCAACCAAAATCAAAAGGTATTTATCGTTTCGAGAATTGCTATTTGAAGCCAAAGTTTCAGGACATGAAGAACAAGCTAGGGTTGACAGAGAATGTCTCGATTTCAACTTGTGGGACAACTGTGCGATCAAATGGTGCAAACAGTTTAATTTTTAATGAGAGCGTCAGTGATGATGACGATGAATCCAAGAACAGACGTTTCAAAGGGGTATGGATCCAACCGGCATTATCTCAGAACTCCTTCAAAGATGATGAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

353

Amino Acids

40.18

Weight (kDa)

6.17

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 107 - 226 3.3e-13 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 222, 547
AclWI GGATC 2 cut(s) 1006, 1019
AcsI RAATTY 4 cut(s) 289, 326, 461, 767
AfaI GTAC 1 cut(s) 696
AfiI CCNNNNNNNGG 2 cut(s) 349, 472
AgsI TTSAA 9 cut(s) 13, 400, 635, 709, 731, 844, 903, 1000, 1042
AluBI AGCT 6 cut(s) 133, 172, 230, 609, 874, 1055
AluI AGCT 6 cut(s) 133, 172, 230, 609, 874, 1055
Alw26I GTCTC 4 cut(s) 344, 513, 585, 898
AlwI GGATC 2 cut(s) 1006, 1019
Ama87I CYCGRG 1 cut(s) 627
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 2 cut(s) 207, 549
ApoI RAATTY 4 cut(s) 289, 326, 461, 767
Asp700I GAANNNNTTC 1 cut(s) 1037
AspLEI GCGC 2 cut(s) 207, 212
AspS9I GGNCC 2 cut(s) 260, 475
AsuC2I CCSGG 2 cut(s) 344, 596
AsuHPI GGTGA 1 cut(s) 472
AvaI CYCGRG 1 cut(s) 627
AvaII GGWCC 2 cut(s) 260, 475
BamHI GGATCC 1 cut(s) 1011
BarI GAAGNNNNNNTAC 1 cut(s) 37
BbvI GCAGC 2 cut(s) 194, 561
BccI CCATC 1 cut(s) 347
BcgI CGANNNNNNTGC 4 cut(s) 473, 507, 726, 760
BclI TGATCA 1 cut(s) 790
BcnI CCSGG 2 cut(s) 344, 596
BcoDI GTCTC 4 cut(s) 344, 513, 585, 898
BfaI CTAG 1 cut(s) 875
BfoI RGCGCY 1 cut(s) 208
BisI GCNGC 3 cut(s) 208, 547, 550
BlsI GCNGC 3 cut(s) 209, 548, 551
Bme1390I CCNGG 2 cut(s) 344, 596
Bme18I GGWCC 2 cut(s) 260, 475
BmeT110I CYCGRG 1 cut(s) 627
BmgT120I GGNCC 2 cut(s) 260, 475
BmiI GGNNCC 3 cut(s) 358, 412, 1013
BmrFI CCNGG 2 cut(s) 344, 596
BmsI GCATC 1 cut(s) 503
BplI GAGNNNNNCTC 2 cut(s) 878, 910
Bpu10I CCTNAGC 1 cut(s) 129
BpuMI CCSGG 2 cut(s) 344, 596
Bsa29I ATCGAT 1 cut(s) 736
BsaI GGTCTC 1 cut(s) 585
BsaJI CCNNGG 3 cut(s) 200, 263, 564
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 472
Bse118I RCCGGY 1 cut(s) 1018
Bse1I ACTGG 1 cut(s) 354
BseCI ATCGAT 1 cut(s) 736
BseDI CCNNGG 3 cut(s) 200, 263, 564
BseGI GGATG 2 cut(s) 358, 574
BseLI CCNNNNNNNGG 2 cut(s) 349, 472
BseMII CTCAG 3 cut(s) 120, 167, 1043
BseNI ACTGG 1 cut(s) 354
BseXI GCAGC 2 cut(s) 194, 561
BsgI GTGCAG 1 cut(s) 177
BshFI GGCC 1 cut(s) 162
BshVI ATCGAT 1 cut(s) 736
BsiHKCI CYCGRG 1 cut(s) 627
BsiSI CCGG 4 cut(s) 344, 442, 595, 1019
BslFI GGGAC 1 cut(s) 925
BslI CCNNNNNNNGG 2 cut(s) 349, 472
BsmAI GTCTC 4 cut(s) 344, 513, 585, 898
BsmBI CGTCTC 1 cut(s) 513
BsmFI GGGAC 1 cut(s) 925
BsnI GGCC 1 cut(s) 162
Bso31I GGTCTC 1 cut(s) 585
BsoBI CYCGRG 1 cut(s) 627
Bsp1407I TGTACA 1 cut(s) 694
Bsp143I GATC 6 cut(s) 118, 484, 737, 790, 923, 1011
BspACI CCGC 2 cut(s) 222, 547
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 3 cut(s) 121, 168, 1042
BspDI ATCGAT 1 cut(s) 736
BspLI GGNNCC 3 cut(s) 358, 412, 1013
BspPI GGATC 2 cut(s) 1006, 1019
BspTNI GGTCTC 1 cut(s) 585
BsrFI RCCGGY 1 cut(s) 1018
BsrGI TGTACA 1 cut(s) 694
BsrI ACTGG 1 cut(s) 354
BssAI RCCGGY 1 cut(s) 1018
BssECI CCNNGG 3 cut(s) 200, 263, 564
BssMI GATC 6 cut(s) 118, 484, 737, 790, 923, 1011
BssT1I CCWWGG 3 cut(s) 200, 263, 564
Bst4CI ACNGT 4 cut(s) 334, 582, 919, 941
BstAPI GCANNNNNTGC 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 694
BstC8I GCNNGC 3 cut(s) 42, 46, 160
BstDEI CTNAG 4 cut(s) 129, 176, 1029, 1056
BstF5I GGATG 2 cut(s) 358, 574
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 2 cut(s) 207, 212
BstKTI GATC 6 cut(s) 121, 487, 740, 793, 926, 1014
BstMAI GTCTC 4 cut(s) 344, 513, 585, 898
BstMBI GATC 6 cut(s) 118, 484, 737, 790, 923, 1011
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 342, 594
BstV1I GCAGC 2 cut(s) 194, 561
BstX2I RGATCY 1 cut(s) 1011
BstYI RGATCY 1 cut(s) 1011
Bsu15I ATCGAT 1 cut(s) 736
BsuRI GGCC 1 cut(s) 162
BsuTUI ATCGAT 1 cut(s) 736
BtsCI GGATG 2 cut(s) 358, 574
BtsI GCAGTG 1 cut(s) 559
BtsIMutI CAGTG 4 cut(s) 330, 559, 578, 970
Cac8I GCNNGC 3 cut(s) 42, 46, 160
CfoI GCGC 2 cut(s) 207, 212
Cfr10I RCCGGY 1 cut(s) 1018
Cfr13I GGNCC 2 cut(s) 260, 475
ClaI ATCGAT 1 cut(s) 736
CseI GACGC 1 cut(s) 949
Csp6I GTAC 1 cut(s) 695
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 8 cut(s) 111, 245, 384, 415, 488, 623, 698, 862
CviQI GTAC 1 cut(s) 695
DdeI CTNAG 4 cut(s) 129, 176, 1029, 1056
DpnI GATC 6 cut(s) 120, 486, 739, 792, 925, 1013
DpnII GATC 6 cut(s) 118, 484, 737, 790, 923, 1011
Eco130I CCWWGG 3 cut(s) 200, 263, 564
Eco147I AGGCCT 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 585
Eco47I GGWCC 2 cut(s) 260, 475
Eco88I CYCGRG 1 cut(s) 627
EcoO109I RGGNCCY 1 cut(s) 260
EcoRI GAATTC 1 cut(s) 767
EcoT14I CCWWGG 3 cut(s) 200, 263, 564
EcoT22I ATGCAT 1 cut(s) 385
ErhI CCWWGG 3 cut(s) 200, 263, 564
Esp3I CGTCTC 1 cut(s) 513
FaeI CATG 8 cut(s) 114, 248, 387, 418, 491, 626, 701, 865
FaqI GGGAC 1 cut(s) 925
FatI CATG 8 cut(s) 110, 244, 383, 414, 487, 622, 697, 861
FbaI TGATCA 1 cut(s) 790
Fnu4HI GCNGC 3 cut(s) 208, 547, 550
FokI GGATG 2 cut(s) 365, 581
Fsp4HI GCNGC 3 cut(s) 208, 547, 550
FspBI CTAG 1 cut(s) 875
GlaI GCGC 2 cut(s) 206, 211
GluI GCNGC 3 cut(s) 208, 547, 550
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 1 cut(s) 162
HapII CCGG 4 cut(s) 344, 442, 595, 1019
HgaI GACGC 1 cut(s) 949
HhaI GCGC 2 cut(s) 207, 212
Hin1II CATG 8 cut(s) 114, 248, 387, 418, 491, 626, 701, 865
Hin6I GCGC 2 cut(s) 205, 210
HinP1I GCGC 2 cut(s) 205, 210
HincII GTYRAC 1 cut(s) 882
HindII GTYRAC 1 cut(s) 882
HindIII AAGCTT 2 cut(s) 607, 1053
HinfI GANTC 2 cut(s) 337, 980
HpaII CCGG 4 cut(s) 344, 442, 595, 1019
HphI GGTGA 1 cut(s) 472
Hpy166II GTNNAC 1 cut(s) 882
Hpy188I TCNGA 6 cut(s) 118, 177, 295, 475, 795, 1032
Hpy188III TCNNGA 5 cut(s) 128, 669, 829, 857, 895
Hpy8I GTNNAC 1 cut(s) 882
HpyAV CCTTC 2 cut(s) 464, 1048
HpyCH4III ACNGT 4 cut(s) 334, 582, 919, 941
HpyCH4IV ACGT 2 cut(s) 154, 994
HpyCH4V TGCA 6 cut(s) 158, 195, 371, 383, 494, 935
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 4 cut(s) 129, 176, 1029, 1056
HpySE526I ACGT 2 cut(s) 154, 994
Hsp92II CATG 8 cut(s) 114, 248, 387, 418, 491, 626, 701, 865
HspAI GCGC 2 cut(s) 205, 210
Ksp22I TGATCA 1 cut(s) 790
Kzo9I GATC 6 cut(s) 118, 484, 737, 790, 923, 1011
LmnI GCTCC 2 cut(s) 85, 169
Lsp1109I GCAGC 2 cut(s) 194, 561
LweI GCATC 1 cut(s) 503
MaeI CTAG 1 cut(s) 875
MaeII ACGT 2 cut(s) 154, 994
MaeIII GTNAC 1 cut(s) 55
MalI GATC 6 cut(s) 120, 486, 739, 792, 925, 1013
MboI GATC 6 cut(s) 118, 484, 737, 790, 923, 1011
MboII GAAGA 6 cut(s) 73, 207, 412, 424, 673, 877
MflI RGATCY 1 cut(s) 1011
MluCI AATT 7 cut(s) 289, 326, 461, 767, 786, 832, 945
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 2 cut(s) 536, 1039
MnlI CCTC 5 cut(s) 135, 274, 401, 488, 775
Mph1103I ATGCAT 1 cut(s) 385
MroXI GAANNNNTTC 1 cut(s) 1037
MseI TTAA 6 cut(s) 35, 605, 611, 618, 944, 951
MspI CCGG 4 cut(s) 344, 442, 595, 1019
MspR9I CCNGG 2 cut(s) 344, 596
MteI GCGCNGCGC 1 cut(s) 208
MwoI GCNNNNNNNGC 1 cut(s) 155
NciI CCSGG 2 cut(s) 344, 596
NdeII GATC 6 cut(s) 118, 484, 737, 790, 923, 1011
NlaIII CATG 8 cut(s) 114, 248, 387, 418, 491, 626, 701, 865
NlaIV GGNNCC 3 cut(s) 358, 412, 1013
NmuCI GTSAC 1 cut(s) 55
NsiI ATGCAT 1 cut(s) 385
PceI AGGCCT 1 cut(s) 162
PcsI WCGNNNNNNNCGW 1 cut(s) 777
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 1 cut(s) 980
PfoI TCCNGGA 1 cut(s) 342
PkrI GCNGC 3 cut(s) 209, 548, 551
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
PpuMI RGGWCCY 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 260
PspN4I GGNNCC 3 cut(s) 358, 412, 1013
PspPI GGNCC 2 cut(s) 260, 475
PspPPI RGGWCCY 1 cut(s) 260
PsuI RGATCY 1 cut(s) 1011
RsaI GTAC 1 cut(s) 696
RsaNI GTAC 1 cut(s) 695
SaqAI TTAA 6 cut(s) 35, 605, 611, 618, 944, 951
SatI GCNGC 3 cut(s) 208, 547, 550
Sau3AI GATC 6 cut(s) 118, 484, 737, 790, 923, 1011
Sau96I GGNCC 2 cut(s) 260, 475
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 2 cut(s) 344, 596
SfaNI GCATC 1 cut(s) 503
SinI GGWCC 2 cut(s) 260, 475
Sse9I AATT 7 cut(s) 289, 326, 461, 767, 786, 832, 945
SseBI AGGCCT 1 cut(s) 162
SsiI CCGC 2 cut(s) 222, 547
SspI AATATT 1 cut(s) 675
SspMI CTAG 1 cut(s) 875
StuI AGGCCT 1 cut(s) 162
StyD4I CCNGG 2 cut(s) 342, 594
StyI CCWWGG 3 cut(s) 200, 263, 564
TaaI ACNGT 4 cut(s) 334, 582, 919, 941
TaiI ACGT 2 cut(s) 157, 997
TaqI TCGA 3 cut(s) 736, 828, 896
TasI AATT 7 cut(s) 289, 326, 461, 767, 786, 832, 945
TatI WGTACW 1 cut(s) 694
TauI GCSGC 1 cut(s) 549
TfiI GAWTC 1 cut(s) 980
Tru1I TTAA 6 cut(s) 35, 605, 611, 618, 944, 951
Tru9I TTAA 6 cut(s) 35, 605, 611, 618, 944, 951
TscAI CASTG 4 cut(s) 337, 559, 585, 970
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 2 cut(s) 207, 549
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 6 cut(s) 261, 492, 573, 715, 878, 993
TspGWI ACGGA 1 cut(s) 98
TspRI CASTG 4 cut(s) 337, 559, 585, 970
VpaK11BI GGWCC 2 cut(s) 260, 475
XapI RAATTY 4 cut(s) 289, 326, 461, 767
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 1 cut(s) 875
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.