FvH4_2g33451

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002267
Physical Location & Seq
Reverse (-)
25092253 .. 25095553
3301 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33451.t1

Sequence Viewer

Length: 1263 bp
ATGGTAGACAATGTCTTTAACCCTGAAATTCAAATACCTAAACTATGCACTTATGCAGTTACTGAGGAATGCTTCACCAAAAATGACATCACTTTTGCTAACCGCCCCGGGCTGCTGGCCGGAAAACACCTTGGATATAACTACACCACCCTTGTCTGGGCCTCTTATGGCTCTCACTGGCGCAACTTGAGACGCATAGCTTCCCTTGAGCTATTGTCATCTCACCGCCTTCAGTTGTTTCATGGCATACGTGCAGATGAAGTCAGGTCATTGATTTTATGCCTTTTCGGAGGTTCCATTGGTGGTGAGTTTCACATTGTGGACATGAAGTCGACGTTTTTTGAGCTGACTCTTAATGTTTTGATGAGGGTGATTGCCGGAAAAAGATACTACGGGGAAGACAAGACAGAACGGGAGGAAGCTAAGAGGTTTAAGCAGATGGTGGTAGAGACATTTCAATTGAGTGGAGCTACTAACATTAGAGATTATGTGCCGTTTTTGAAATATGGTAGAGCAAATGGGCTTGAGAAAAAGCTGTTGGTATTGCAAGAGAAGAGGGACAAGTTCATGCAAGATTTGATTGAAGAACATAGAAAAGGGCAGAGTGGCTCTGTTTCTGAACAGAGAAGCAAGACCATGGTGGATGTGTTGCTCTCACTGCAAGAAACTGAACCTGAATATTACACTGATGTGATCATAAGAGGCCTCATGCAAGTGCTTTTGCGCCTACCATACTCCGATGAAAATTTACACTTCAAAAATGTCACACAGGTGATGTTATCAGCAGGAACTGACACTTCAGCTGGAACCATGGAATGGGGGCTTGCACTTCTGCTCAACAACCCAGAAGCCCTAGCAAAAGCACAGACAGAAATTGATGTCCAAATTGGGCAAAGCAGGCTAATTGAGGAAGCAGACCTTGCCAACCTTCCCTATCTCCATGGCATCATAAACGAGACCCTCAGAATGTACCCAGCAGACCCACTTCTAGTACCCCATGAGTCCTCACAGGAGTGCACACTGGGAGGGTACCATGTCCCACGTGGCACAATGCTGTTGGTGAACATTTGGGCCATGCATCATGATCCCAAATTGTGGGAGCAGCCTGAGCAGTTCAAGCCAGAGAGGTTTTTAACTTCAAAGAATGAAGCAAATGGTTTCAAGCTGCTGCCGTTTGGGACAGGAAGGAGAGGCTGTCCTGGAGAAGGGTTAGCGACTCGAATAATCGGGCTGGCGTTAGGGGTCACTGATACAGTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

421

Amino Acids

47.56

Weight (kDa)

6.21

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 15 - 414 6.1e-73 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1029
AccB1I GGYRCC 1 cut(s) 1029
AccB7I CCANNNNNTGG 2 cut(s) 816, 1095
AccI GTMKAC 2 cut(s) 6, 332
AciI CCGC 2 cut(s) 103, 226
AclWI GGATC 1 cut(s) 1079
AcoI YGGCCR 1 cut(s) 117
AcsI RAATTY 2 cut(s) 27, 745
AcuI CTGAAG 2 cut(s) 215, 783
AcvI CACGTG 1 cut(s) 1043
AdeI CACNNNGTG 1 cut(s) 319
AfaI GTAC 3 cut(s) 971, 993, 1031
AfiI CCNNNNNNNGG 5 cut(s) 156, 157, 816, 1095, 1205
AgsI TTSAA 8 cut(s) 32, 458, 502, 584, 757, 1117, 1140, 1162
AhdI GACNNNNNGTC 1 cut(s) 328
AjnI CCWGG 1 cut(s) 1198
AjuI GAANNNNNNNTTGG 2 cut(s) 521, 553
AleI CACNNNNGTG 3 cut(s) 689, 770, 1012
AluBI AGCT 8 cut(s) 200, 211, 346, 422, 470, 535, 803, 1165
AluI AGCT 8 cut(s) 200, 211, 346, 422, 470, 535, 803, 1165
Alw21I GWGCWC 1 cut(s) 1019
Alw26I GTCTC 3 cut(s) 184, 443, 950
Alw44I GTGCAC 1 cut(s) 1015
AlwI GGATC 1 cut(s) 1079
AlwNI CAGNNNCTG 2 cut(s) 62, 791
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 4 cut(s) 117, 159, 703, 1071
ApaLI GTGCAC 1 cut(s) 1015
ApeKI GCWGC 4 cut(s) 112, 1102, 1165, 1168
ApoI RAATTY 2 cut(s) 27, 745
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
Asp718I GGTACC 1 cut(s) 1029
AspLEI GCGC 2 cut(s) 183, 726
AspS9I GGNCC 2 cut(s) 159, 1071
AsuC2I CCSGG 2 cut(s) 108, 109
AsuHPI GGTGA 6 cut(s) 67, 215, 317, 382, 784, 1072
AvaI CYCGRG 1 cut(s) 107
BaeGI GKGCMC 1 cut(s) 1019
BanI GGYRCC 1 cut(s) 1029
BbrPI CACGTG 1 cut(s) 1043
BbsI GAAGAC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 1019
BbvI GCAGC 4 cut(s) 99, 1114, 1152, 1155
BccI CCATC 1 cut(s) 433
BceAI ACGGC 2 cut(s) 478, 1156
BciT130I CCWGG 1 cut(s) 1200
BclI TGATCA 1 cut(s) 693
BcnI CCSGG 2 cut(s) 108, 109
BcoDI GTCTC 3 cut(s) 184, 443, 950
BfaI CTAG 2 cut(s) 854, 989
BisI GCNGC 4 cut(s) 113, 1103, 1166, 1169
BlsI GCNGC 4 cut(s) 114, 1104, 1167, 1170
Bme1390I CCNGG 3 cut(s) 108, 109, 1200
BmeRI GACNNNNNGTC 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 2 cut(s) 159, 1071
BmiI GGNNCC 3 cut(s) 295, 808, 1031
BmrFI CCNGG 3 cut(s) 108, 109, 1200
BmrI ACTGGG 1 cut(s) 1031
BmsI GCATC 2 cut(s) 954, 1087
BmuI ACTGGG 1 cut(s) 1031
BpiI GAAGAC 1 cut(s) 405
BpmI CTGGAG 1 cut(s) 1221
Bpu10I CCTNAGC 1 cut(s) 1107
BpuEI CTTGAG 3 cut(s) 208, 227, 545
BpuMI CCSGG 2 cut(s) 108, 109
BsaAI YACGTR 2 cut(s) 251, 1043
BsaI GGTCTC 1 cut(s) 950
BsaJI CCNNGG 6 cut(s) 106, 107, 130, 636, 810, 940
BsaXI ACNNNNNCTCC 2 cut(s) 1180, 1210
Bsc4I CCNNNNNNNGG 5 cut(s) 156, 157, 816, 1095, 1205
Bse1I ACTGG 2 cut(s) 182, 1026
BseBI CCWGG 1 cut(s) 1200
BseDI CCNNGG 6 cut(s) 106, 107, 130, 636, 810, 940
BseGI GGATG 1 cut(s) 649
BseLI CCNNNNNNNGG 5 cut(s) 156, 157, 816, 1095, 1205
BseMII CTCAG 3 cut(s) 54, 976, 1098
BseNI ACTGG 2 cut(s) 182, 1026
BseSI GKGCMC 1 cut(s) 1019
BseXI GCAGC 4 cut(s) 99, 1114, 1152, 1155
BseYI CCCAGC 1 cut(s) 973
BsgI GTGCAG 1 cut(s) 273
BshFI GGCC 4 cut(s) 119, 161, 705, 1073
BshNI GGYRCC 1 cut(s) 1029
BsiHKAI GWGCWC 1 cut(s) 1019
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 3 cut(s) 108, 120, 378
BslFI GGGAC 3 cut(s) 572, 1022, 1192
BslI CCNNNNNNNGG 5 cut(s) 156, 157, 816, 1095, 1205
BsmAI GTCTC 3 cut(s) 184, 443, 950
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 3 cut(s) 572, 1022, 1192
BsmI GAATGC 1 cut(s) 74
BsnI GGCC 4 cut(s) 119, 161, 705, 1073
Bso31I GGTCTC 1 cut(s) 950
BsoBI CYCGRG 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 1019
Bsp143I GATC 2 cut(s) 693, 1084
Bsp19I CCATGG 3 cut(s) 636, 810, 940
BspACI CCGC 2 cut(s) 103, 226
BspANI GGCC 4 cut(s) 119, 161, 705, 1073
BspCNI CTCAG 3 cut(s) 55, 975, 1099
BspHI TCATGA 1 cut(s) 1081
BspLI GGNNCC 3 cut(s) 295, 808, 1031
BspPI GGATC 1 cut(s) 1079
BspT107I GGYRCC 1 cut(s) 1029
BspTNI GGTCTC 1 cut(s) 950
BsrI ACTGG 2 cut(s) 182, 1026
BssECI CCNNGG 6 cut(s) 106, 107, 130, 636, 810, 940
BssMI GATC 2 cut(s) 693, 1084
BssT1I CCWWGG 4 cut(s) 130, 636, 810, 940
Bst2UI CCWGG 1 cut(s) 1200
Bst4CI ACNGT 1 cut(s) 1255
Bst6I CTCTTC 1 cut(s) 548
BstAPI GCANNNNNTGC 1 cut(s) 920
BstBAI YACGTR 2 cut(s) 251, 1043
BstC8I GCNNGC 4 cut(s) 117, 825, 899, 1233
BstDEI CTNAG 4 cut(s) 63, 423, 962, 1107
BstDSI CCRYGG 3 cut(s) 636, 810, 940
BstENI CCTNNNNNAGG 1 cut(s) 1203
BstF5I GGATG 1 cut(s) 649
BstHHI GCGC 2 cut(s) 183, 726
BstKTI GATC 2 cut(s) 696, 1087
BstMAI GTCTC 3 cut(s) 184, 443, 950
BstMBI GATC 2 cut(s) 693, 1084
BstMWI GCNNNNNNNGC 5 cut(s) 658, 898, 920, 1108, 1117
BstNI CCWGG 1 cut(s) 1200
BstSCI CCNGG 3 cut(s) 106, 107, 1198
BstSLI GKGCMC 1 cut(s) 1019
BstV1I GCAGC 4 cut(s) 99, 1114, 1152, 1155
BstV2I GAAGAC 1 cut(s) 405
BsuRI GGCC 4 cut(s) 119, 161, 705, 1073
BtgI CCRYGG 3 cut(s) 636, 810, 940
BtsCI GGATG 1 cut(s) 649
BtsI GCAGTG 1 cut(s) 656
BtsIMutI CAGTG 6 cut(s) 175, 656, 684, 1019, 1245, 1260
Cac8I GCNNGC 4 cut(s) 117, 825, 899, 1233
CaiI CAGNNNCTG 2 cut(s) 62, 791
CciI TCATGA 1 cut(s) 1081
CfoI GCGC 2 cut(s) 183, 726
Cfr13I GGNCC 2 cut(s) 159, 1071
Cfr9I CCCGGG 1 cut(s) 107
CseI GACGC 1 cut(s) 201
Csp6I GTAC 3 cut(s) 970, 992, 1030
CviQI GTAC 3 cut(s) 970, 992, 1030
DdeI CTNAG 4 cut(s) 63, 423, 962, 1107
DpnI GATC 2 cut(s) 695, 1086
DpnII GATC 2 cut(s) 693, 1084
DraIII CACNNNGTG 1 cut(s) 319
DriI GACNNNNNGTC 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 117
Eam1104I CTCTTC 1 cut(s) 548
Eam1105I GACNNNNNGTC 1 cut(s) 328
EarI CTCTTC 1 cut(s) 548
Eco130I CCWWGG 4 cut(s) 130, 636, 810, 940
Eco147I AGGCCT 1 cut(s) 705
Eco31I GGTCTC 1 cut(s) 950
Eco57I CTGAAG 2 cut(s) 215, 783
Eco72I CACGTG 1 cut(s) 1043
Eco88I CYCGRG 1 cut(s) 107
EcoNI CCTNNNNNAGG 1 cut(s) 1203
EcoRII CCWGG 1 cut(s) 1198
EcoT14I CCWWGG 4 cut(s) 130, 636, 810, 940
EcoT22I ATGCAT 1 cut(s) 1080
ErhI CCWWGG 4 cut(s) 130, 636, 810, 940
Esp3I CGTCTC 1 cut(s) 184
FalI AAGNNNNNCTT 2 cut(s) 903, 935
FaqI GGGAC 3 cut(s) 572, 1022, 1192
FbaI TGATCA 1 cut(s) 693
FblI GTMKAC 2 cut(s) 6, 332
Fnu4HI GCNGC 4 cut(s) 113, 1103, 1166, 1169
FokI GGATG 1 cut(s) 656
Fsp4HI GCNGC 4 cut(s) 113, 1103, 1166, 1169
FspBI CTAG 2 cut(s) 854, 989
GlaI GCGC 2 cut(s) 182, 725
GluI GCNGC 4 cut(s) 113, 1103, 1166, 1169
GsaI CCCAGC 1 cut(s) 977
GsuI CTGGAG 1 cut(s) 1221
HaeIII GGCC 4 cut(s) 119, 161, 705, 1073
HapII CCGG 3 cut(s) 108, 120, 378
HgaI GACGC 1 cut(s) 201
HhaI GCGC 2 cut(s) 183, 726
Hin6I GCGC 2 cut(s) 181, 724
HinP1I GCGC 2 cut(s) 181, 724
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HinfI GANTC 3 cut(s) 349, 1001, 1216
HpaII CCGG 3 cut(s) 108, 120, 378
HphI GGTGA 6 cut(s) 67, 215, 317, 382, 784, 1072
Hpy166II GTNNAC 5 cut(s) 7, 322, 333, 1017, 1063
Hpy188I TCNGA 4 cut(s) 290, 619, 739, 965
Hpy188III TCNNGA 1 cut(s) 1082
Hpy8I GTNNAC 5 cut(s) 7, 322, 333, 1017, 1063
Hpy99I CGWCG 1 cut(s) 337
HpyAV CCTTC 4 cut(s) 239, 938, 1179, 1199
HpyCH4III ACNGT 1 cut(s) 1255
HpyCH4IV ACGT 3 cut(s) 250, 335, 1042
HpyF10VI GCNNNNNNNGC 5 cut(s) 658, 898, 920, 1108, 1117
HpyF3I CTNAG 4 cut(s) 63, 423, 962, 1107
HpySE526I ACGT 3 cut(s) 250, 335, 1042
HspAI GCGC 2 cut(s) 181, 724
KpnI GGTACC 1 cut(s) 1033
Ksp22I TGATCA 1 cut(s) 693
Kzo9I GATC 2 cut(s) 693, 1084
LmnI GCTCC 2 cut(s) 467, 1099
Lsp1109I GCAGC 4 cut(s) 99, 1114, 1152, 1155
LweI GCATC 2 cut(s) 954, 1087
MaeI CTAG 2 cut(s) 854, 989
MaeII ACGT 3 cut(s) 250, 335, 1042
MaeIII GTNAC 3 cut(s) 58, 763, 1243
MalI GATC 2 cut(s) 695, 1086
MboI GATC 2 cut(s) 693, 1084
MboII GAAGA 3 cut(s) 410, 565, 596
MfeI CAATTG 1 cut(s) 458
MhlI GDGCHC 1 cut(s) 1019
MluCI AATT 7 cut(s) 27, 458, 745, 873, 885, 903, 1091
MlyI GAGTC 3 cut(s) 343, 1010, 1210
Mph1103I ATGCAT 1 cut(s) 1080
MseI TTAA 4 cut(s) 18, 354, 432, 1133
MslI CAYNNNNRTG 4 cut(s) 689, 713, 770, 1012
MspA1I CMGCKG 1 cut(s) 803
MspI CCGG 3 cut(s) 108, 120, 378
MspR9I CCNGG 3 cut(s) 108, 109, 1200
MunI CAATTG 1 cut(s) 458
Mva1269I GAATGC 1 cut(s) 74
MvaI CCWGG 1 cut(s) 1200
MwoI GCNNNNNNNGC 5 cut(s) 658, 898, 920, 1108, 1117
NciI CCSGG 2 cut(s) 108, 109
NcoI CCATGG 3 cut(s) 636, 810, 940
NdeII GATC 2 cut(s) 693, 1084
NlaIV GGNNCC 3 cut(s) 295, 808, 1031
NmuCI GTSAC 2 cut(s) 763, 1243
NsiI ATGCAT 1 cut(s) 1080
OliI CACNNNNGTG 3 cut(s) 689, 770, 1012
PagI TCATGA 1 cut(s) 1081
PceI AGGCCT 1 cut(s) 705
PctI GAATGC 1 cut(s) 74
PflFI GACNNNGTC 1 cut(s) 11
PflMI CCANNNNNTGG 2 cut(s) 816, 1095
PfoI TCCNGGA 1 cut(s) 1198
PkrI GCNGC 4 cut(s) 114, 1104, 1167, 1170
PleI GAGTC 3 cut(s) 343, 1009, 1210
PmaCI CACGTG 1 cut(s) 1043
PmlI CACGTG 1 cut(s) 1043
PpsI GAGTC 3 cut(s) 343, 1009, 1210
Ppu21I YACGTR 2 cut(s) 251, 1043
Psp6I CCWGG 1 cut(s) 1198
PspCI CACGTG 1 cut(s) 1043
PspFI CCCAGC 1 cut(s) 973
PspGI CCWGG 1 cut(s) 1198
PspN4I GGNNCC 3 cut(s) 295, 808, 1031
PspPI GGNCC 2 cut(s) 159, 1071
PstNI CAGNNNCTG 2 cut(s) 62, 791
PsyI GACNNNGTC 1 cut(s) 11
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 3 cut(s) 971, 993, 1031
RsaNI GTAC 3 cut(s) 970, 992, 1030
RseI CAYNNNNRTG 4 cut(s) 689, 713, 770, 1012
SalI GTCGAC 1 cut(s) 331
SaqAI TTAA 4 cut(s) 18, 354, 432, 1133
SatI GCNGC 4 cut(s) 113, 1103, 1166, 1169
Sau3AI GATC 2 cut(s) 693, 1084
Sau96I GGNCC 2 cut(s) 159, 1071
SchI GAGTC 3 cut(s) 343, 1010, 1210
ScrFI CCNGG 3 cut(s) 108, 109, 1200
SduI GDGCHC 1 cut(s) 1019
SfaNI GCATC 2 cut(s) 954, 1087
SmaI CCCGGG 1 cut(s) 109
SmiMI CAYNNNNRTG 4 cut(s) 689, 713, 770, 1012
SmlI CTYRAG 3 cut(s) 187, 206, 524
SmoI CTYRAG 3 cut(s) 187, 206, 524
Sse9I AATT 7 cut(s) 27, 458, 745, 873, 885, 903, 1091
SseBI AGGCCT 1 cut(s) 705
SsiI CCGC 2 cut(s) 103, 226
SspI AATATT 1 cut(s) 680
SspMI CTAG 2 cut(s) 854, 989
StuI AGGCCT 1 cut(s) 705
StyD4I CCNGG 3 cut(s) 106, 107, 1198
StyI CCWWGG 4 cut(s) 130, 636, 810, 940
TaaI ACNGT 1 cut(s) 1255
TaiI ACGT 3 cut(s) 253, 338, 1045
TaqI TCGA 2 cut(s) 332, 1219
TasI AATT 7 cut(s) 27, 458, 745, 873, 885, 903, 1091
Tru1I TTAA 4 cut(s) 18, 354, 432, 1133
Tru9I TTAA 4 cut(s) 18, 354, 432, 1133
TscAI CASTG 6 cut(s) 182, 663, 691, 1026, 1252, 1260
TseFI GTSAC 2 cut(s) 763, 1243
TseI GCWGC 4 cut(s) 112, 1102, 1165, 1168
Tsp45I GTSAC 2 cut(s) 763, 1243
TspDTI ATGAA 6 cut(s) 230, 273, 341, 556, 756, 1161
TspMI CCCGGG 1 cut(s) 107
TspRI CASTG 6 cut(s) 182, 663, 691, 1026, 1252, 1260
Tth111I GACNNNGTC 1 cut(s) 11
Van91I CCANNNNNTGG 2 cut(s) 816, 1095
VneI GTGCAC 1 cut(s) 1015
XagI CCTNNNNNAGG 1 cut(s) 1203
XapI RAATTY 2 cut(s) 27, 745
XcmI CCANNNNNNNNNTGG 1 cut(s) 1040
XmaI CCCGGG 1 cut(s) 107
XmiI GTMKAC 2 cut(s) 6, 332
XspI CTAG 2 cut(s) 854, 989
Zsp2I ATGCAT 1 cut(s) 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.