MD08G1090000.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002267
Physical Location & Seq
Forward (+)
7440806 .. 7442962
2157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1090000.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGTTTCACGACATCCGTGCAGATGAAGTTAGGTCATTGATTTGCCTGTTTTTCCGAGGTTCTAACAGCCGTGAATTTCAGAATTTCGAGATGAAGTCAACGTTTTTTGAGCTTACACTTAATGTTCTGATAAGGATAATCGCCGGAAAGCGGTATTACGGAGAACATATGGCGGATTTGGAGGAAGCGAACTGGTTTAAGCGGATTGTGACGGAGACCTTTGAGCTGAGCGGGGCTACGAATATAGGAGATTTCGTGCCAGCTTTGAAGTATTTGGGAGTAAGGGGGCTTGAGAAGAAGTTGGTAATATTGCAGAAGAAAGGGGATGGATTCATGCAAGATTTGATTGAAGAACATAGAAAATTGCAGAGTAGCTCTGTTTCTGAACAGAAGAGGAAGACAAAGGTGGAAGTTTTGCTTTCCCTGCAAGAAACTGAACCTGAATATTATACAGATGAGATCATAAGAGGCATCATGCAAACTGAAATTGATAACCACATTGGACAAAGCTGCAGGCTACTTGAGGAATCAGACCTTCCCAAGTTACCCTATCTCCAAGGCATCATCAGTGAAACCCTCCGAATGTACCCAACAGGCCCATTGCTAGCCCCCCACGAGTCAGTAAAGGACTGTACCGTGGGGGGCTTCCACGTCCCACGTGGAACAATGTTGTTCATGAACATATGGGCCATACAACATAATCCAAAGTTGTGGGCACAACCTGACCAATTCAAGCCAGAGAGGTTCCATAACCTACAAGAGGAAAGAGATGGTTTTGTTTGGTTGCCGTTTGGGGCAGGGAGGAGGGGCTGCCCCGGGGAAGGCTTAGCGACTCGGATAGTCGGGCTGACTCTAGGATCACTGATTCAGTGTTTTGAGTGGGAGAGAATTGGTGAGGAAATGGTGGACATGCGTGAAGGGACTGGTCTCACCATGCCTAGAGCTCAACCTTTACTTGCAAAGTGCAGGCCACGCCCAAAAATGCTTGCCCTACTATCTCAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

336

Amino Acids

38.48

Weight (kDa)

6.29

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 159 - 314 3.7e-48 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 231
AciI CCGC 4 cut(s) 151, 173, 202, 231
AclI AACGTT 1 cut(s) 101
AclWI GGATC 1 cut(s) 865
AcsI RAATTY 2 cut(s) 74, 82
AcvI CACGTG 1 cut(s) 659
AfaI GTAC 2 cut(s) 587, 634
AfiI CCNNNNNNNGG 3 cut(s) 150, 760, 821
AgsI TTSAA 3 cut(s) 268, 350, 733
AjiI CACGTC 1 cut(s) 652
AluBI AGCT 6 cut(s) 112, 226, 263, 375, 510, 944
AluI AGCT 6 cut(s) 112, 226, 263, 375, 510, 944
Alw21I GWGCWC 1 cut(s) 946
Alw26I GTCTC 2 cut(s) 209, 932
AlwI GGATC 1 cut(s) 865
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 3 cut(s) 595, 687, 968
ApeKI GCWGC 2 cut(s) 510, 810
ApoI RAATTY 2 cut(s) 74, 82
AspS9I GGNCC 2 cut(s) 596, 687
AsuC2I CCSGG 2 cut(s) 816, 817
AsuHPI GGTGA 2 cut(s) 905, 922
AsuNHI GCTAGC 1 cut(s) 604
AvaI CYCGRG 1 cut(s) 815
BaeGI GKGCMC 1 cut(s) 718
BanII GRGCYC 1 cut(s) 946
BauI CACGAG 1 cut(s) 614
BbrPI CACGTG 1 cut(s) 659
BbsI GAAGAC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 946
BbvI GCAGC 2 cut(s) 497, 797
BccI CCATC 2 cut(s) 320, 764
BceAI ACGGC 2 cut(s) 54, 772
BcgI CGANNNNNNTGC 1 cut(s) 33
BcnI CCSGG 2 cut(s) 816, 817
BcoDI GTCTC 2 cut(s) 209, 932
BfaI CTAG 3 cut(s) 605, 854, 939
BfmI CTRYAG 1 cut(s) 511
BisI GCNGC 2 cut(s) 511, 811
BlpI GCTNAGC 2 cut(s) 227, 826
BlsI GCNGC 2 cut(s) 512, 812
Bme1390I CCNGG 2 cut(s) 816, 817
BmeT110I CYCGRG 1 cut(s) 815
BmgBI CACGTC 1 cut(s) 652
BmgT120I GGNCC 2 cut(s) 596, 687
BmiI GGNNCC 1 cut(s) 746
BmrFI CCNGG 2 cut(s) 816, 817
BmsI GCATC 2 cut(s) 480, 570
BmtI GCTAGC 1 cut(s) 608
BpiI GAAGAC 1 cut(s) 404
Bpu1102I GCTNAGC 2 cut(s) 227, 826
BpuEI CTTGAG 2 cut(s) 311, 542
BpuMI CCSGG 2 cut(s) 816, 817
BsaAI YACGTR 1 cut(s) 659
BsaI GGTCTC 2 cut(s) 209, 932
BsaJI CCNNGG 6 cut(s) 55, 556, 636, 814, 815, 816
BsaXI ACNNNNNCTCC 4 cut(s) 240, 270, 537, 567
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 760, 821
Bse1I ACTGG 2 cut(s) 197, 928
Bse3DI GCAATG 1 cut(s) 599
BseDI CCNNGG 6 cut(s) 55, 556, 636, 814, 815, 816
BseGI GGATG 2 cut(s) 12, 331
BseLI CCNNNNNNNGG 3 cut(s) 150, 760, 821
BseMI GCAATG 1 cut(s) 599
BseMII CTCAG 1 cut(s) 218
BseNI ACTGG 2 cut(s) 197, 928
BseRI GAGGAG 1 cut(s) 817
BseSI GKGCMC 1 cut(s) 718
BseXI GCAGC 2 cut(s) 497, 797
BsgI GTGCAG 2 cut(s) 39, 985
BshFI GGCC 3 cut(s) 597, 689, 970
BsiHKAI GWGCWC 1 cut(s) 946
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 2 cut(s) 144, 816
BslFI GGGAC 2 cut(s) 638, 934
BslI CCNNNNNNNGG 3 cut(s) 150, 760, 821
BsmAI GTCTC 2 cut(s) 209, 932
BsmFI GGGAC 2 cut(s) 638, 934
BsnI GGCC 3 cut(s) 597, 689, 970
Bso31I GGTCTC 2 cut(s) 209, 932
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 2 cut(s) 718, 946
Bsp143I GATC 2 cut(s) 459, 857
Bsp1720I GCTNAGC 2 cut(s) 227, 826
BspACI CCGC 4 cut(s) 151, 173, 202, 231
BspANI GGCC 3 cut(s) 597, 689, 970
BspCNI CTCAG 1 cut(s) 219
BspHI TCATGA 1 cut(s) 675
BspLI GGNNCC 1 cut(s) 746
BspMAI CTGCAG 1 cut(s) 515
BspOI GCTAGC 1 cut(s) 608
BspPI GGATC 1 cut(s) 865
BspTNI GGTCTC 2 cut(s) 209, 932
BsrBI CCGCTC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 599
BsrI ACTGG 2 cut(s) 197, 928
BssECI CCNNGG 6 cut(s) 55, 556, 636, 814, 815, 816
BssMI GATC 2 cut(s) 459, 857
BssSI CACGAG 1 cut(s) 614
BssT1I CCWWGG 1 cut(s) 556
Bst2BI CACGAG 1 cut(s) 614
Bst4CI ACNGT 2 cut(s) 632, 637
Bst6I CTCTTC 1 cut(s) 386
BstBAI YACGTR 1 cut(s) 659
BstC8I GCNNGC 5 cut(s) 261, 515, 606, 968, 987
BstDEI CTNAG 2 cut(s) 227, 826
BstDSI CCRYGG 1 cut(s) 636
BstENI CCTNNNNNAGG 1 cut(s) 758
BstF5I GGATG 2 cut(s) 12, 331
BstKTI GATC 2 cut(s) 462, 860
BstMAI GTCTC 2 cut(s) 209, 932
BstMBI GATC 2 cut(s) 459, 857
BstMWI GCNNNNNNNGC 2 cut(s) 424, 972
BstNSI RCATGY 1 cut(s) 913
BstSCI CCNGG 2 cut(s) 814, 815
BstSFI CTRYAG 1 cut(s) 511
BstSLI GKGCMC 1 cut(s) 718
BstV1I GCAGC 2 cut(s) 497, 797
BstV2I GAAGAC 1 cut(s) 404
BstXI CCANNNNNNTGG 1 cut(s) 711
BsuRI GGCC 3 cut(s) 597, 689, 970
BtgI CCRYGG 1 cut(s) 636
BtrI CACGTC 1 cut(s) 652
BtsCI GGATG 2 cut(s) 12, 331
BtsIMutI CAGTG 3 cut(s) 574, 860, 875
Cac8I GCNNGC 5 cut(s) 261, 515, 606, 968, 987
CciI TCATGA 1 cut(s) 675
Cfr13I GGNCC 2 cut(s) 596, 687
Cfr9I CCCGGG 1 cut(s) 815
Csp6I GTAC 2 cut(s) 586, 633
CviAII CATG 5 cut(s) 334, 475, 676, 910, 934
CviQI GTAC 2 cut(s) 586, 633
DdeI CTNAG 2 cut(s) 227, 826
DpnI GATC 2 cut(s) 461, 859
DpnII GATC 2 cut(s) 459, 857
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 944
Eco130I CCWWGG 1 cut(s) 556
Eco24I GRGCYC 1 cut(s) 946
Eco31I GGTCTC 2 cut(s) 209, 932
Eco53kI GAGCTC 1 cut(s) 944
Eco72I CACGTG 1 cut(s) 659
Eco88I CYCGRG 1 cut(s) 815
EcoICRI GAGCTC 1 cut(s) 944
EcoNI CCTNNNNNAGG 1 cut(s) 758
EcoT14I CCWWGG 1 cut(s) 556
EcoT38I GRGCYC 1 cut(s) 946
ErhI CCWWGG 1 cut(s) 556
FaeI CATG 5 cut(s) 337, 478, 679, 913, 937
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 2 cut(s) 638, 934
FatI CATG 5 cut(s) 333, 474, 675, 909, 933
FauI CCCGC 1 cut(s) 224
FauNDI CATATG 2 cut(s) 168, 683
Fnu4HI GCNGC 2 cut(s) 511, 811
FokI GGATG 1 cut(s) 338
FriOI GRGCYC 1 cut(s) 946
Fsp4HI GCNGC 2 cut(s) 511, 811
FspBI CTAG 3 cut(s) 605, 854, 939
GluI GCNGC 2 cut(s) 511, 811
HaeIII GGCC 3 cut(s) 597, 689, 970
HapII CCGG 2 cut(s) 144, 816
Hin1II CATG 5 cut(s) 337, 478, 679, 913, 937
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HinfI GANTC 6 cut(s) 330, 527, 617, 832, 850, 865
HpaII CCGG 2 cut(s) 144, 816
HphI GGTGA 2 cut(s) 905, 922
Hpy166II GTNNAC 2 cut(s) 99, 907
Hpy188I TCNGA 7 cut(s) 56, 81, 129, 385, 532, 581, 837
Hpy188III TCNNGA 3 cut(s) 8, 88, 676
Hpy8I GTNNAC 2 cut(s) 99, 907
HpyAV CCTTC 3 cut(s) 545, 815, 911
HpyCH4III ACNGT 2 cut(s) 632, 637
HpyCH4IV ACGT 3 cut(s) 101, 651, 658
HpyCH4V TGCA 9 cut(s) 20, 313, 337, 367, 427, 478, 513, 959, 966
HpyF10VI GCNNNNNNNGC 2 cut(s) 424, 972
HpyF3I CTNAG 2 cut(s) 227, 826
HpySE526I ACGT 3 cut(s) 101, 651, 658
Hsp92II CATG 5 cut(s) 337, 478, 679, 913, 937
Kzo9I GATC 2 cut(s) 459, 857
Lsp1109I GCAGC 2 cut(s) 497, 797
LweI GCATC 2 cut(s) 480, 570
MaeI CTAG 3 cut(s) 605, 854, 939
MaeII ACGT 3 cut(s) 101, 651, 658
MaeIII GTNAC 2 cut(s) 208, 543
MalI GATC 2 cut(s) 461, 859
MbiI CCGCTC 1 cut(s) 231
MboI GATC 2 cut(s) 459, 857
MboII GAAGA 5 cut(s) 307, 328, 362, 403, 409
MhlI GDGCHC 2 cut(s) 718, 946
MluCI AATT 6 cut(s) 74, 82, 362, 486, 728, 888
MlyI GAGTC 3 cut(s) 626, 826, 844
MseI TTAA 2 cut(s) 120, 198
MspI CCGG 2 cut(s) 144, 816
MspR9I CCNGG 2 cut(s) 816, 817
MwoI GCNNNNNNNGC 2 cut(s) 424, 972
NciI CCSGG 2 cut(s) 816, 817
NdeI CATATG 2 cut(s) 168, 683
NdeII GATC 2 cut(s) 459, 857
NheI GCTAGC 1 cut(s) 604
NlaIII CATG 5 cut(s) 337, 478, 679, 913, 937
NlaIV GGNNCC 1 cut(s) 746
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 1 cut(s) 913
PagI TCATGA 1 cut(s) 675
PfeI GAWTC 3 cut(s) 330, 527, 865
PkrI GCNGC 2 cut(s) 512, 812
PleI GAGTC 3 cut(s) 625, 826, 844
PmaCI CACGTG 1 cut(s) 659
PmlI CACGTG 1 cut(s) 659
PpsI GAGTC 3 cut(s) 625, 826, 844
Ppu21I YACGTR 1 cut(s) 659
Psp124BI GAGCTC 1 cut(s) 946
Psp1406I AACGTT 1 cut(s) 101
PspCI CACGTG 1 cut(s) 659
PspN4I GGNNCC 1 cut(s) 746
PspPI GGNCC 2 cut(s) 596, 687
PstI CTGCAG 1 cut(s) 515
RsaI GTAC 2 cut(s) 587, 634
RsaNI GTAC 2 cut(s) 586, 633
SacI GAGCTC 1 cut(s) 946
SaqAI TTAA 2 cut(s) 120, 198
SatI GCNGC 2 cut(s) 511, 811
Sau3AI GATC 2 cut(s) 459, 857
Sau96I GGNCC 2 cut(s) 596, 687
SchI GAGTC 3 cut(s) 626, 826, 844
ScrFI CCNGG 2 cut(s) 816, 817
SduI GDGCHC 2 cut(s) 718, 946
SfaNI GCATC 2 cut(s) 480, 570
SfcI CTRYAG 1 cut(s) 511
SmaI CCCGGG 1 cut(s) 817
SmlI CTYRAG 2 cut(s) 290, 521
SmoI CTYRAG 2 cut(s) 290, 521
Sse9I AATT 6 cut(s) 74, 82, 362, 486, 728, 888
SsiI CCGC 4 cut(s) 151, 173, 202, 231
SspI AATATT 2 cut(s) 309, 446
SspMI CTAG 3 cut(s) 605, 854, 939
SstI GAGCTC 1 cut(s) 946
StyD4I CCNGG 2 cut(s) 814, 815
StyI CCWWGG 1 cut(s) 556
TaaI ACNGT 2 cut(s) 632, 637
TaiI ACGT 3 cut(s) 104, 654, 661
TaqI TCGA 1 cut(s) 87
TasI AATT 6 cut(s) 74, 82, 362, 486, 728, 888
TfiI GAWTC 3 cut(s) 330, 527, 865
Tru1I TTAA 2 cut(s) 120, 198
Tru9I TTAA 2 cut(s) 120, 198
TscAI CASTG 3 cut(s) 574, 867, 875
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 2 cut(s) 510, 810
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 5 cut(s) 39, 107, 322, 664, 692
TspGWI ACGGA 3 cut(s) 5, 174, 227
TspMI CCCGGG 1 cut(s) 815
TspRI CASTG 3 cut(s) 574, 867, 875
XagI CCTNNNNNAGG 1 cut(s) 758
XapI RAATTY 2 cut(s) 74, 82
XceI RCATGY 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 1 cut(s) 656
XmaI CCCGGG 1 cut(s) 815
XspI CTAG 3 cut(s) 605, 854, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.