Rh6AG482200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002267
Physical Location & Seq
Forward (+)
66254802 .. 66256048
1247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG482200.1

Sequence Viewer

Length: 966 bp
ATGAAGTACATCAGCTTCAGAGTTGCAGAGATCAACATAGCCATGGAAACCTCATTCCATTACTACATTTTGCTCCTCATTATTTATCTTCTCCTCAAGAACTACTTGCAGAAAAATAACAAAAGACTCCCACCAAGCCCTTCTCTATCTTTCCCTATCATAGGTCATCTCTGCCTCATCAAGAAACCCCTCCACAGAACACTTGCAAAATTAGCCAACAAATATGGATCAATCTTGTACATCAAGTGCGGCTCCCGCCCTGTCGTCATTGTATCTTCTCCTTCTGCAGCTGAGGAATGCTTCACCAAAAATGACATTGCTTTTGCTAACCGCCCCGGGCTGCTTGCCGGAAAACACCTAGGATATAAATACACCACCCTTGTCTGGGCCTCTTATGGCTCTCACTGGCGCAACTTGAGGCGCATAGCTTCCCTTGAGCTATTGTCATCTCACCGCCTTCAGATGTTTCATGGAATACGTGCAGATGAAGTCAGATCATTGATTTTACGCCTTTTCCGAGGTTCCATTGGTGGTGAGTTTTACATTGTGGACATGAAGTCGACGTTTTTTCAGCTGACTCTTAATGTTATCATGAGGATGATTGCTGGAAAAAGATACTATGAGGAAGGCAGGACAGGATTGGAGGAAGCTAAGAGGTTTGAACAGATTGTGGTAGAGACATTTCAATTGAGTGGAGCTACTAACATTGGAGATTTTGTACCAGTTTTGAAATATGTTGGATCAAATGGGCTTGAAAAAAAGTTGTTGATATTGCAAAAGAAGAGGGACAAGTTCATGCAAGATTTGATTGAAGAACATAGAAGAGGGCAGAGTGGCTCTGTTTCTGAACAGAGGAGCAAGACCATGGTGGATGTGTTGCTGTCACTGCAAGAAACTGAACCTGAATACTACACTGATGAAATCATAAGAGGCCTGATGCAAGTAAGCTTCCTCACTCAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

321

Amino Acids

36.89

Weight (kDa)

9.64

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 45 - 311 1.3e-27 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 560
AciI CCGC 4 cut(s) 249, 256, 331, 454
AclWI GGATC 2 cut(s) 235, 748
AcuI CTGAAG 1 cut(s) 443
AfaI GTAC 3 cut(s) 8, 239, 720
AfiI CCNNNNNNNGG 3 cut(s) 161, 384, 385
AgsI TTSAA 5 cut(s) 662, 686, 730, 755, 812
AhdI GACNNNNNGTC 1 cut(s) 556
AluBI AGCT 8 cut(s) 15, 290, 428, 439, 574, 650, 698, 948
AluI AGCT 8 cut(s) 15, 290, 428, 439, 574, 650, 698, 948
Alw26I GTCTC 1 cut(s) 671
AlwI GGATC 2 cut(s) 235, 748
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 2 cut(s) 387, 931
ApeKI GCWGC 2 cut(s) 287, 340
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AspA2I CCTAGG 1 cut(s) 358
AspLEI GCGC 2 cut(s) 411, 423
AspS9I GGNCC 1 cut(s) 387
AsuC2I CCSGG 2 cut(s) 336, 337
AsuHPI GGTGA 3 cut(s) 295, 443, 545
AvaI CYCGRG 1 cut(s) 335
AvrII CCTAGG 1 cut(s) 358
BarI GAAGNNNNNNTAC 1 cut(s) 31
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 2 cut(s) 299, 327
BcnI CCSGG 2 cut(s) 336, 337
BcoDI GTCTC 1 cut(s) 671
BfaI CTAG 2 cut(s) 359, 964
BfmI CTRYAG 1 cut(s) 285
BisI GCNGC 3 cut(s) 250, 288, 341
BlnI CCTAGG 1 cut(s) 358
BlsI GCNGC 3 cut(s) 251, 289, 342
Bme1390I CCNGG 2 cut(s) 336, 337
BmeRI GACNNNNNGTC 1 cut(s) 556
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 387
BmiI GGNNCC 2 cut(s) 253, 523
BmrFI CCNGG 2 cut(s) 336, 337
BmsI GCATC 1 cut(s) 927
Bpu10I CCTNAGC 1 cut(s) 291
BpuEI CTTGAG 3 cut(s) 80, 436, 455
BpuMI CCSGG 2 cut(s) 336, 337
BsaAI YACGTR 1 cut(s) 479
BsaJI CCNNGG 6 cut(s) 42, 334, 335, 358, 517, 864
BsaXI ACNNNNNCTCC 2 cut(s) 702, 732
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 384, 385
Bse1I ACTGG 2 cut(s) 410, 722
Bse3DI GCAATG 1 cut(s) 315
BseDI CCNNGG 6 cut(s) 42, 334, 335, 358, 517, 864
BseGI GGATG 2 cut(s) 603, 877
BseLI CCNNNNNNNGG 3 cut(s) 161, 384, 385
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 2 cut(s) 410, 722
BseRI GAGGAG 3 cut(s) 65, 83, 868
BseXI GCAGC 2 cut(s) 299, 327
BsgI GTGCAG 1 cut(s) 501
BshFI GGCC 2 cut(s) 389, 933
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 2 cut(s) 336, 348
BslFI GGGAC 1 cut(s) 800
BslI CCNNNNNNNGG 3 cut(s) 161, 384, 385
BsmAI GTCTC 1 cut(s) 671
BsmFI GGGAC 1 cut(s) 800
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 2 cut(s) 389, 933
BsoBI CYCGRG 1 cut(s) 335
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 4 cut(s) 30, 227, 494, 740
Bsp19I CCATGG 2 cut(s) 42, 864
BspACI CCGC 4 cut(s) 249, 256, 331, 454
BspANI GGCC 2 cut(s) 389, 933
BspCNI CTCAG 1 cut(s) 283
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 2 cut(s) 253, 523
BspMAI CTGCAG 1 cut(s) 289
BspPI GGATC 2 cut(s) 235, 748
BsrDI GCAATG 1 cut(s) 315
BsrGI TGTACA 1 cut(s) 237
BsrI ACTGG 2 cut(s) 410, 722
BssECI CCNNGG 6 cut(s) 42, 334, 335, 358, 517, 864
BssMI GATC 4 cut(s) 30, 227, 494, 740
BssT1I CCWWGG 3 cut(s) 42, 358, 864
Bst6I CTCTTC 2 cut(s) 776, 817
BstAUI TGTACA 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 479
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 2 cut(s) 291, 651
BstDSI CCRYGG 2 cut(s) 42, 864
BstENI CCTNNNNNAGG 1 cut(s) 159
BstF5I GGATG 2 cut(s) 603, 877
BstHHI GCGC 2 cut(s) 411, 423
BstKTI GATC 4 cut(s) 33, 230, 497, 743
BstMAI GTCTC 1 cut(s) 671
BstMBI GATC 4 cut(s) 30, 227, 494, 740
BstMWI GCNNNNNNNGC 3 cut(s) 212, 255, 886
BstSCI CCNGG 2 cut(s) 334, 335
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 2 cut(s) 299, 327
BsuRI GGCC 2 cut(s) 389, 933
BtgI CCRYGG 2 cut(s) 42, 864
BtsCI GGATG 2 cut(s) 603, 877
BtsI GCAGTG 1 cut(s) 884
BtsIMutI CAGTG 3 cut(s) 403, 884, 912
Cac8I GCNNGC 1 cut(s) 345
CciI TCATGA 1 cut(s) 591
CfoI GCGC 2 cut(s) 411, 423
Cfr13I GGNCC 1 cut(s) 387
Cfr9I CCCGGG 1 cut(s) 335
Csp6I GTAC 3 cut(s) 7, 238, 719
CviAII CATG 6 cut(s) 43, 470, 553, 592, 796, 865
CviQI GTAC 3 cut(s) 7, 238, 719
DdeI CTNAG 2 cut(s) 291, 651
DpnI GATC 4 cut(s) 32, 229, 496, 742
DpnII GATC 4 cut(s) 30, 227, 494, 740
DriI GACNNNNNGTC 1 cut(s) 556
Eam1104I CTCTTC 2 cut(s) 776, 817
Eam1105I GACNNNNNGTC 1 cut(s) 556
EarI CTCTTC 2 cut(s) 776, 817
Eco130I CCWWGG 3 cut(s) 42, 358, 864
Eco147I AGGCCT 1 cut(s) 933
Eco57I CTGAAG 1 cut(s) 443
Eco88I CYCGRG 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 159
EcoT14I CCWWGG 3 cut(s) 42, 358, 864
ErhI CCWWGG 3 cut(s) 42, 358, 864
FaeI CATG 6 cut(s) 46, 473, 556, 595, 799, 868
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 800
FatI CATG 6 cut(s) 42, 469, 552, 591, 795, 864
FauI CCCGC 1 cut(s) 263
FblI GTMKAC 1 cut(s) 560
Fnu4HI GCNGC 3 cut(s) 250, 288, 341
FokI GGATG 2 cut(s) 610, 884
Fsp4HI GCNGC 3 cut(s) 250, 288, 341
FspBI CTAG 2 cut(s) 359, 964
GlaI GCGC 2 cut(s) 410, 422
GluI GCNGC 3 cut(s) 250, 288, 341
HaeIII GGCC 2 cut(s) 389, 933
HapII CCGG 2 cut(s) 336, 348
HhaI GCGC 2 cut(s) 411, 423
Hin1II CATG 6 cut(s) 46, 473, 556, 595, 799, 868
Hin6I GCGC 2 cut(s) 409, 421
HinP1I GCGC 2 cut(s) 409, 421
HincII GTYRAC 1 cut(s) 561
HindII GTYRAC 1 cut(s) 561
HindIII AAGCTT 1 cut(s) 946
HinfI GANTC 2 cut(s) 126, 577
HpaII CCGG 2 cut(s) 336, 348
HphI GGTGA 3 cut(s) 295, 443, 545
Hpy166II GTNNAC 2 cut(s) 550, 561
Hpy188I TCNGA 5 cut(s) 20, 462, 494, 518, 847
Hpy188III TCNNGA 3 cut(s) 97, 181, 592
Hpy8I GTNNAC 2 cut(s) 550, 561
Hpy99I CGWCG 1 cut(s) 565
HpyAV CCTTC 4 cut(s) 150, 291, 467, 620
HpyCH4IV ACGT 2 cut(s) 478, 563
HpyCH4V TGCA 9 cut(s) 26, 109, 206, 287, 482, 775, 799, 889, 940
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 255, 886
HpyF3I CTNAG 2 cut(s) 291, 651
HpySE526I ACGT 2 cut(s) 478, 563
Hsp92II CATG 6 cut(s) 46, 473, 556, 595, 799, 868
HspAI GCGC 2 cut(s) 409, 421
Kzo9I GATC 4 cut(s) 30, 227, 494, 740
LmnI GCTCC 4 cut(s) 78, 257, 695, 855
Lsp1109I GCAGC 2 cut(s) 299, 327
LweI GCATC 1 cut(s) 927
MaeI CTAG 2 cut(s) 359, 964
MaeII ACGT 2 cut(s) 478, 563
MaeIII GTNAC 1 cut(s) 882
MalI GATC 4 cut(s) 32, 229, 496, 742
MboI GATC 4 cut(s) 30, 227, 494, 740
MboII GAAGA 5 cut(s) 80, 267, 793, 824, 834
MfeI CAATTG 1 cut(s) 686
MluCI AATT 2 cut(s) 209, 686
MlyI GAGTC 2 cut(s) 120, 571
MmeI TCCRAC 1 cut(s) 718
MseI TTAA 1 cut(s) 582
MslI CAYNNNNRTG 2 cut(s) 41, 596
MspA1I CMGCKG 2 cut(s) 290, 574
MspI CCGG 2 cut(s) 336, 348
MspR9I CCNGG 2 cut(s) 336, 337
MunI CAATTG 1 cut(s) 686
Mva1269I GAATGC 1 cut(s) 302
MwoI GCNNNNNNNGC 3 cut(s) 212, 255, 886
NciI CCSGG 2 cut(s) 336, 337
NcoI CCATGG 2 cut(s) 42, 864
NdeII GATC 4 cut(s) 30, 227, 494, 740
NlaIII CATG 6 cut(s) 46, 473, 556, 595, 799, 868
NlaIV GGNNCC 2 cut(s) 253, 523
NmuCI GTSAC 1 cut(s) 882
PagI TCATGA 1 cut(s) 591
PceI AGGCCT 1 cut(s) 933
PcsI WCGNNNNNNNCGW 1 cut(s) 514
PctI GAATGC 1 cut(s) 302
PkrI GCNGC 3 cut(s) 251, 289, 342
PleI GAGTC 2 cut(s) 120, 571
PpsI GAGTC 2 cut(s) 120, 571
Ppu21I YACGTR 1 cut(s) 479
PspN4I GGNNCC 2 cut(s) 253, 523
PspPI GGNCC 1 cut(s) 387
PstI CTGCAG 1 cut(s) 289
PvuII CAGCTG 2 cut(s) 290, 574
RsaI GTAC 3 cut(s) 8, 239, 720
RsaNI GTAC 3 cut(s) 7, 238, 719
RseI CAYNNNNRTG 2 cut(s) 41, 596
SalI GTCGAC 1 cut(s) 559
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 3 cut(s) 250, 288, 341
Sau3AI GATC 4 cut(s) 30, 227, 494, 740
Sau96I GGNCC 1 cut(s) 387
SchI GAGTC 2 cut(s) 120, 571
ScrFI CCNGG 2 cut(s) 336, 337
SfaNI GCATC 1 cut(s) 927
SfcI CTRYAG 1 cut(s) 285
SmaI CCCGGG 1 cut(s) 337
SmiMI CAYNNNNRTG 2 cut(s) 41, 596
SmlI CTYRAG 3 cut(s) 95, 415, 434
SmoI CTYRAG 3 cut(s) 95, 415, 434
Sse9I AATT 2 cut(s) 209, 686
SseBI AGGCCT 1 cut(s) 933
SsiI CCGC 4 cut(s) 249, 256, 331, 454
SspMI CTAG 2 cut(s) 359, 964
StuI AGGCCT 1 cut(s) 933
StyD4I CCNGG 2 cut(s) 334, 335
StyI CCWWGG 3 cut(s) 42, 358, 864
TaiI ACGT 2 cut(s) 481, 566
TaqI TCGA 1 cut(s) 560
TasI AATT 2 cut(s) 209, 686
TatI WGTACW 2 cut(s) 6, 237
TauI GCSGC 1 cut(s) 252
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 3 cut(s) 410, 891, 919
TseFI GTSAC 1 cut(s) 882
TseI GCWGC 2 cut(s) 287, 340
Tsp45I GTSAC 1 cut(s) 882
TspDTI ATGAA 6 cut(s) 17, 458, 501, 569, 784, 933
TspMI CCCGGG 1 cut(s) 335
TspRI CASTG 3 cut(s) 410, 891, 919
XagI CCTNNNNNAGG 1 cut(s) 159
XmaI CCCGGG 1 cut(s) 335
XmaJI CCTAGG 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 560
XspI CTAG 2 cut(s) 359, 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.