Rw6G041960

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002267
Physical Location & Seq
Reverse (-)
65609204 .. 65610893
1690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041960.1

Sequence Viewer

Length: 1521 bp
ATGGAAACTTCAACCTACTCCTACATCTTGCTCTTCATCATCTTTCTTTACCTCAAGAGTTATTTGCAGAAAATTAAGAAAAGACTCCCACCAAGCCCTTCTCCTTCCCTTCCCATCATAGGTCATCTCTACCTATTTAAGAAACCTCTCCACAGAACACTCGCAAAACTAACTGACAAATATGGTCCAATTTTATACCTTCAATTTGGATCCCGCCCGGTTGTTGTTGTATCCTCTCCTTCTGCAGTTGAGGAATGTTTCACCAAAAACGATGTCACTTTCGCAAACCGTCCAAAGCTGCTTGCCGGCAAGCACTTTGGATGCAACTATACTGCGATTGTCTGGGCTTCCTATGGCTCTCACTGGCGCAACTTGAGACGCATAGCTTCCCTTGAGCTATTGTCATCCAGCCGCCTTCAGATGTTTCATGGCATACGTGCAGATGAAGTTAGGTCACTAATTTGCAAGCTTTTTCGAGGGTATAATGGTGGTGAACTGCGGAGTTTAGATATGAAGTCAACGTTTTTCGAGCTTACTCTTAATGTTTTGATGAGGGTGATTGCTGGGAAGAGATACTATGGTGAAGACATCGCAGGGTCCAGCGAAGCTAAGAGTTTTGAACAAATTGTGAAAGAGACTTTTCAATTGAGTGGGGCTAACAATATTGGAGATTTCTTGCCAGTTTTAAAGTACCTTGGATCAGGTGGCCTTGAAAAAAAGATGTTGAAATTACAGGAGAAGAGGGATAAGTTCATGCAATATTTGATTGAAGAACACAGAAAAGTGCAGAGTGGCTCTGTTTCTGAACAGAAGAGCAAGACCATGGTGGATGTCTTGTTGTCACTGCAAGCAACTGAACCTGAATATTATACTGATGAAATCATAAGAGGCATCATGCAAAACATGTTAACAGCAGGAACAGACACCTCGGCTGGAACCATGGAATGGGCTCTATCACTATTGCTCAACAACCCAGAAGCCCTTGCAAAGGCACAGACAGAAATTGATGACAAAATTGGGCAAAGCAGGCTAATTGAGGAATCAGACCTTGCTGATCTCCCCTATCTCCATTGCATCATAAACGAGACCCTCAGAATGTACCCAGCAGCCGCACTTCTTCCCCCACACGAGTCATCAGAGGACTGCACCGTGGGAGGCTTCAATGTTCCTCGTGGAACAATGCTGTTGGTGAACGCATGGGCCATACATCATGATCCTAAGTTGTGGGAAGAGCCTGAACAATTTAAGCCAGAGAGGTTCCAAAATGCACAAGAAGAAAGAGATGTTTTCAAGTACTTGCCATTTGGCACAGGAAGAAGAGGCTGTCCTGGGGAAGGGTTAGCCAACCGGATGGTAGGGCTGGCCTTAGGGTCAGTCATTCAGTGTTTTGAGTGGGAGAGAAGTGGTGAGGAGATGGTGGACATGACTGAAGGGACTGGCATCTCAATGCCTAGAGCTCACCCTTTGCTAGCAAAATGCAGGCCACGCCCAACGATGCTGGCCTTACTTTCTCAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

506

Amino Acids

57.13

Weight (kDa)

8.26

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 31 - 485 1.2e-102 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 945
AciI CCGC 4 cut(s) 214, 412, 499, 1110
AclI AACGTT 1 cut(s) 521
AclWI GGATC 4 cut(s) 204, 217, 706, 1208
AcuI CTGAAG 2 cut(s) 401, 1449
AfaI GTAC 3 cut(s) 692, 1100, 1295
AfiI CCNNNNNNNGG 4 cut(s) 119, 945, 988, 1334
AflIII ACRYGT 1 cut(s) 903
AgsI TTSAA 9 cut(s) 12, 203, 620, 644, 713, 727, 770, 1162, 1291
AjnI CCWGG 1 cut(s) 1327
AluBI AGCT 7 cut(s) 298, 386, 397, 469, 532, 608, 1457
AluI AGCT 7 cut(s) 298, 386, 397, 469, 532, 608, 1457
Alw21I GWGCWC 1 cut(s) 1459
Alw26I GTCTC 3 cut(s) 370, 629, 1079
AlwI GGATC 4 cut(s) 204, 217, 706, 1208
AoxI GGCC 5 cut(s) 706, 1200, 1362, 1481, 1500
ApeKI GCWGC 2 cut(s) 298, 1106
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 3 cut(s) 185, 597, 1200
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 7 cut(s) 253, 503, 568, 593, 1201, 1418, 1451
AsuNHI GCTAGC 1 cut(s) 1468
AvaII GGWCC 2 cut(s) 185, 597
AxyI CCTNAGG 1 cut(s) 1366
BaeI ACNNNNGTAYC 2 cut(s) 565, 598
BamHI GGATCC 1 cut(s) 209
BanII GRGCYC 2 cut(s) 952, 1459
BauI CACGAG 2 cut(s) 1127, 1170
BbsI GAAGAC 1 cut(s) 591
Bbv12I GWGCWC 1 cut(s) 1459
BbvI GCAGC 2 cut(s) 285, 1118
BccI CCATC 3 cut(s) 122, 1345, 1408
BciT130I CCWGG 1 cut(s) 1329
BciVI GTATCC 1 cut(s) 241
BcnI CCSGG 1 cut(s) 218
BcoDI GTCTC 3 cut(s) 370, 629, 1079
BfaI CTAG 2 cut(s) 1452, 1469
BfmI CTRYAG 1 cut(s) 243
BfuI GTATCC 1 cut(s) 241
BisI GCNGC 4 cut(s) 299, 412, 1107, 1110
BlsI GCNGC 4 cut(s) 300, 413, 1108, 1111
BmcAI AGTACT 1 cut(s) 1295
Bme1390I CCNGG 2 cut(s) 218, 1329
Bme18I GGWCC 2 cut(s) 185, 597
BmgT120I GGNCC 3 cut(s) 185, 597, 1200
BmiI GGNNCC 4 cut(s) 211, 598, 937, 1259
BmrFI CCNGG 2 cut(s) 218, 1329
BmsI GCATC 5 cut(s) 311, 900, 1083, 1449, 1485
BmtI GCTAGC 1 cut(s) 1472
BpiI GAAGAC 1 cut(s) 591
BpuEI CTTGAG 3 cut(s) 38, 394, 413
BpuMI CCSGG 1 cut(s) 218
BsaAI YACGTR 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 1079
BsaJI CCNNGG 6 cut(s) 694, 822, 927, 939, 1149, 1328
BsaWI WCCGGW 1 cut(s) 1347
Bsc4I CCNNNNNNNGG 4 cut(s) 119, 945, 988, 1334
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 3 cut(s) 368, 680, 1441
Bse21I CCTNAGG 1 cut(s) 1366
Bse3DI GCAATG 1 cut(s) 1069
BseBI CCWGG 1 cut(s) 1329
BseDI CCNNGG 6 cut(s) 694, 822, 927, 939, 1149, 1328
BseGI GGATG 4 cut(s) 326, 404, 835, 1356
BseLI CCNNNNNNNGG 4 cut(s) 119, 945, 988, 1334
BseMI GCAATG 1 cut(s) 1069
BseMII CTCAG 1 cut(s) 1105
BseNI ACTGG 3 cut(s) 368, 680, 1441
BseRI GAGGAG 1 cut(s) 1424
BseXI GCAGC 2 cut(s) 285, 1118
BseYI CCCAGC 2 cut(s) 563, 1102
BsgI GTGCAG 3 cut(s) 459, 806, 1129
BshFI GGCC 5 cut(s) 708, 1202, 1364, 1483, 1502
BsiHKAI GWGCWC 1 cut(s) 1459
BsiSI CCGG 3 cut(s) 218, 306, 1348
BslFI GGGAC 1 cut(s) 1447
BslI CCNNNNNNNGG 4 cut(s) 119, 945, 988, 1334
BsmAI GTCTC 3 cut(s) 370, 629, 1079
BsmBI CGTCTC 1 cut(s) 370
BsmFI GGGAC 1 cut(s) 1447
BsnI GGCC 5 cut(s) 708, 1202, 1364, 1483, 1502
Bso31I GGTCTC 1 cut(s) 1079
Bsp1286I GDGCHC 2 cut(s) 952, 1459
Bsp143I GATC 4 cut(s) 209, 698, 1054, 1213
Bsp19I CCATGG 2 cut(s) 822, 939
BspACI CCGC 4 cut(s) 214, 412, 499, 1110
BspANI GGCC 5 cut(s) 708, 1202, 1364, 1483, 1502
BspCNI CTCAG 1 cut(s) 1104
BspHI TCATGA 1 cut(s) 1210
BspLI GGNNCC 4 cut(s) 211, 598, 937, 1259
BspMAI CTGCAG 1 cut(s) 247
BspOI GCTAGC 1 cut(s) 1472
BspPI GGATC 4 cut(s) 204, 217, 706, 1208
BspQI GCTCTTC 3 cut(s) 38, 806, 1224
BspTNI GGTCTC 1 cut(s) 1079
BsrDI GCAATG 1 cut(s) 1069
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 3 cut(s) 368, 680, 1441
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 6 cut(s) 694, 822, 927, 939, 1149, 1328
BssMI GATC 4 cut(s) 209, 698, 1054, 1213
BssSI CACGAG 2 cut(s) 1127, 1170
BssT1I CCWWGG 3 cut(s) 694, 822, 939
Bst2BI CACGAG 2 cut(s) 1127, 1170
Bst2UI CCWGG 1 cut(s) 1329
Bst4CI ACNGT 2 cut(s) 290, 1150
Bst6I CTCTTC 6 cut(s) 38, 563, 734, 806, 1224, 1312
BstBAI YACGTR 1 cut(s) 437
BstDEI CTNAG 4 cut(s) 609, 1091, 1218, 1366
BstDSI CCRYGG 3 cut(s) 822, 939, 1149
BstENI CCTNNNNNAGG 2 cut(s) 986, 1332
BstF5I GGATG 4 cut(s) 326, 404, 835, 1356
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 4 cut(s) 212, 701, 1057, 1216
BstMAI GTCTC 3 cut(s) 370, 629, 1079
BstMBI GATC 4 cut(s) 209, 698, 1054, 1213
BstMWI GCNNNNNNNGC 2 cut(s) 1027, 1485
BstNI CCWGG 1 cut(s) 1329
BstNSI RCATGY 1 cut(s) 907
BstSCI CCNGG 2 cut(s) 216, 1327
BstSFI CTRYAG 1 cut(s) 243
BstV1I GCAGC 2 cut(s) 285, 1118
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 1 cut(s) 209
BstXI CCANNNNNNTGG 1 cut(s) 1351
BstYI RGATCY 1 cut(s) 209
Bsu36I CCTNAGG 1 cut(s) 1366
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 5 cut(s) 708, 1202, 1364, 1483, 1502
BtgI CCRYGG 3 cut(s) 822, 939, 1149
BtgZI GCGATG 1 cut(s) 574
BtsCI GGATG 4 cut(s) 326, 404, 835, 1356
BtsI GCAGTG 1 cut(s) 842
BtsIMutI CAGTG 3 cut(s) 361, 842, 1388
CciI TCATGA 1 cut(s) 1210
CfoI GCGC 1 cut(s) 369
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 3 cut(s) 185, 597, 1200
CseI GACGC 1 cut(s) 387
Csp6I GTAC 3 cut(s) 691, 1099, 1294
CviAII CATG 9 cut(s) 428, 754, 823, 895, 904, 940, 1197, 1211, 1423
CviQI GTAC 3 cut(s) 691, 1099, 1294
DdeI CTNAG 4 cut(s) 609, 1091, 1218, 1366
DpnI GATC 4 cut(s) 211, 700, 1056, 1215
DpnII GATC 4 cut(s) 209, 698, 1054, 1213
DraI TTTAAA 1 cut(s) 687
Eam1104I CTCTTC 6 cut(s) 38, 563, 734, 806, 1224, 1312
EarI CTCTTC 6 cut(s) 38, 563, 734, 806, 1224, 1312
Ecl136II GAGCTC 1 cut(s) 1457
Eco130I CCWWGG 3 cut(s) 694, 822, 939
Eco24I GRGCYC 2 cut(s) 952, 1459
Eco31I GGTCTC 1 cut(s) 1079
Eco47I GGWCC 2 cut(s) 185, 597
Eco53kI GAGCTC 1 cut(s) 1457
Eco57I CTGAAG 2 cut(s) 401, 1449
Eco81I CCTNAGG 1 cut(s) 1366
EcoICRI GAGCTC 1 cut(s) 1457
EcoNI CCTNNNNNAGG 2 cut(s) 986, 1332
EcoRII CCWGG 1 cut(s) 1327
EcoT14I CCWWGG 3 cut(s) 694, 822, 939
EcoT38I GRGCYC 2 cut(s) 952, 1459
ErhI CCWWGG 3 cut(s) 694, 822, 939
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 9 cut(s) 431, 757, 826, 898, 907, 943, 1200, 1214, 1426
FaqI GGGAC 1 cut(s) 1447
FatI CATG 9 cut(s) 427, 753, 822, 894, 903, 939, 1196, 1210, 1422
FauI CCCGC 1 cut(s) 221
Fnu4HI GCNGC 4 cut(s) 299, 412, 1107, 1110
FokI GGATG 4 cut(s) 333, 391, 842, 1363
FriOI GRGCYC 2 cut(s) 952, 1459
Fsp4HI GCNGC 4 cut(s) 299, 412, 1107, 1110
FspBI CTAG 2 cut(s) 1452, 1469
GlaI GCGC 1 cut(s) 368
GluI GCNGC 4 cut(s) 299, 412, 1107, 1110
GsaI CCCAGC 2 cut(s) 567, 1106
HaeIII GGCC 5 cut(s) 708, 1202, 1364, 1483, 1502
HapII CCGG 3 cut(s) 218, 306, 1348
HgaI GACGC 1 cut(s) 387
HhaI GCGC 1 cut(s) 369
Hin1II CATG 9 cut(s) 431, 757, 826, 898, 907, 943, 1200, 1214, 1426
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HincII GTYRAC 2 cut(s) 519, 909
HindII GTYRAC 2 cut(s) 519, 909
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 3 cut(s) 84, 1040, 1130
HpaI GTTAAC 1 cut(s) 909
HpaII CCGG 3 cut(s) 218, 306, 1348
HphI GGTGA 7 cut(s) 253, 503, 568, 593, 1201, 1418, 1451
Hpy166II GTNNAC 5 cut(s) 494, 519, 909, 1192, 1420
Hpy188I TCNGA 5 cut(s) 420, 805, 1045, 1094, 1138
Hpy188III TCNNGA 2 cut(s) 55, 1211
Hpy8I GTNNAC 5 cut(s) 494, 519, 909, 1192, 1420
HpyAV CCTTC 8 cut(s) 108, 114, 119, 209, 249, 425, 1328, 1424
HpyCH4III ACNGT 2 cut(s) 290, 1150
HpyCH4IV ACGT 2 cut(s) 436, 521
HpyF10VI GCNNNNNNNGC 2 cut(s) 1027, 1485
HpyF3I CTNAG 4 cut(s) 609, 1091, 1218, 1366
HpySE526I ACGT 2 cut(s) 436, 521
Hsp92II CATG 9 cut(s) 431, 757, 826, 898, 907, 943, 1200, 1214, 1426
HspAI GCGC 1 cut(s) 367
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
KspAI GTTAAC 1 cut(s) 909
Kzo9I GATC 4 cut(s) 209, 698, 1054, 1213
LguI GCTCTTC 3 cut(s) 38, 806, 1224
Lsp1109I GCAGC 2 cut(s) 285, 1118
LweI GCATC 5 cut(s) 311, 900, 1083, 1449, 1485
MaeI CTAG 2 cut(s) 1452, 1469
MaeII ACGT 2 cut(s) 436, 521
MaeIII GTNAC 3 cut(s) 274, 453, 840
MalI GATC 4 cut(s) 211, 700, 1056, 1215
MboI GATC 4 cut(s) 209, 698, 1054, 1213
MfeI CAATTG 1 cut(s) 644
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 2 cut(s) 952, 1459
MlyI GAGTC 2 cut(s) 78, 1139
MroNI GCCGGC 1 cut(s) 305
MseI TTAA 6 cut(s) 75, 138, 540, 686, 908, 1245
MslI CAYNNNNRTG 1 cut(s) 1445
MspI CCGG 3 cut(s) 218, 306, 1348
MspR9I CCNGG 2 cut(s) 218, 1329
MunI CAATTG 1 cut(s) 644
MvaI CCWGG 1 cut(s) 1329
MwoI GCNNNNNNNGC 2 cut(s) 1027, 1485
NaeI GCCGGC 1 cut(s) 307
NciI CCSGG 1 cut(s) 218
NcoI CCATGG 2 cut(s) 822, 939
NdeII GATC 4 cut(s) 209, 698, 1054, 1213
NgoMIV GCCGGC 1 cut(s) 305
NheI GCTAGC 1 cut(s) 1468
NlaIII CATG 9 cut(s) 431, 757, 826, 898, 907, 943, 1200, 1214, 1426
NlaIV GGNNCC 4 cut(s) 211, 598, 937, 1259
NmeAIII GCCGAG 1 cut(s) 908
NmuCI GTSAC 3 cut(s) 274, 453, 840
NspI RCATGY 1 cut(s) 907
PagI TCATGA 1 cut(s) 1210
PciI ACATGT 1 cut(s) 903
PciSI GCTCTTC 3 cut(s) 38, 806, 1224
PdiI GCCGGC 1 cut(s) 307
PfeI GAWTC 1 cut(s) 1040
PflMI CCANNNNNTGG 1 cut(s) 945
PkrI GCNGC 4 cut(s) 300, 413, 1108, 1111
PleI GAGTC 2 cut(s) 78, 1138
PpsI GAGTC 2 cut(s) 78, 1138
Ppu21I YACGTR 1 cut(s) 437
PscI ACATGT 1 cut(s) 903
Psp124BI GAGCTC 1 cut(s) 1459
Psp1406I AACGTT 1 cut(s) 521
Psp6I CCWGG 1 cut(s) 1327
PspFI CCCAGC 2 cut(s) 563, 1102
PspGI CCWGG 1 cut(s) 1327
PspN4I GGNNCC 4 cut(s) 211, 598, 937, 1259
PspPI GGNCC 3 cut(s) 185, 597, 1200
PstI CTGCAG 1 cut(s) 247
PsuI RGATCY 1 cut(s) 209
RsaI GTAC 3 cut(s) 692, 1100, 1295
RsaNI GTAC 3 cut(s) 691, 1099, 1294
RseI CAYNNNNRTG 1 cut(s) 1445
SacI GAGCTC 1 cut(s) 1459
SapI GCTCTTC 3 cut(s) 38, 806, 1224
SaqAI TTAA 6 cut(s) 75, 138, 540, 686, 908, 1245
SatI GCNGC 4 cut(s) 299, 412, 1107, 1110
Sau3AI GATC 4 cut(s) 209, 698, 1054, 1213
Sau96I GGNCC 3 cut(s) 185, 597, 1200
ScaI AGTACT 1 cut(s) 1295
SchI GAGTC 2 cut(s) 78, 1139
ScrFI CCNGG 2 cut(s) 218, 1329
SduI GDGCHC 2 cut(s) 952, 1459
SfaNI GCATC 5 cut(s) 311, 900, 1083, 1449, 1485
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 2 cut(s) 185, 597
SmiMI CAYNNNNRTG 1 cut(s) 1445
SmlI CTYRAG 3 cut(s) 53, 373, 392
SmoI CTYRAG 3 cut(s) 53, 373, 392
SsiI CCGC 4 cut(s) 214, 412, 499, 1110
SspI AATATT 3 cut(s) 664, 761, 866
SspMI CTAG 2 cut(s) 1452, 1469
SstI GAGCTC 1 cut(s) 1459
StyD4I CCNGG 2 cut(s) 216, 1327
StyI CCWWGG 3 cut(s) 694, 822, 939
TaaI ACNGT 2 cut(s) 290, 1150
TaiI ACGT 2 cut(s) 439, 524
TaqI TCGA 2 cut(s) 475, 528
TatI WGTACW 1 cut(s) 1293
TauI GCSGC 2 cut(s) 414, 1112
TfiI GAWTC 1 cut(s) 1040
Tru1I TTAA 6 cut(s) 75, 138, 540, 686, 908, 1245
Tru9I TTAA 6 cut(s) 75, 138, 540, 686, 908, 1245
TscAI CASTG 3 cut(s) 368, 849, 1388
TseFI GTSAC 3 cut(s) 274, 453, 840
TseI GCWGC 2 cut(s) 298, 1106
Tsp45I GTSAC 3 cut(s) 274, 453, 840
TspDTI ATGAA 6 cut(s) 25, 416, 459, 527, 742, 891
TspRI CASTG 3 cut(s) 368, 849, 1388
Van91I CCANNNNNTGG 1 cut(s) 945
VpaK11BI GGWCC 2 cut(s) 185, 597
XagI CCTNNNNNAGG 2 cut(s) 986, 1332
XceI RCATGY 1 cut(s) 907
XspI CTAG 2 cut(s) 1452, 1469
ZrmI AGTACT 1 cut(s) 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.