pycom15g06970

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002267
Physical Location & Seq
Forward (+)
4248569 .. 4250618
2050 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g06970.2

Sequence Viewer

Length: 1413 bp
ATGTATGGATGGAGCACCCCTTATCTTAAGAAGTACACACAGCTTGCAGACATCAGCATATCAATGGAAACTTCGTTCTTCTTCTTCTTCTTCTTCTACTTTTTGCTTTTCATTTTCTTTCTTTTCCTCAAAAACTATTTGCAGAAAATTAACAAAAAACTCCCACCAAGCCCCTCTCTTTGTTTCCCAGTCATAGGCCATCTCTACCTCTTCAAAAAACCCCTCCACAGAACACTTGCCAAACTCTCCAACAAACATGGACCAATATCATATCTCCGATTAGGCTCCCGCCCGGTCCTCCTCGTATCTTCACCGTCCGCGGCTGAGGAATGCTTCACCAAAAACGACATCACTTTTGCCAACCGCCCTAAGTTCCTCGCAGGAAAACACCTCGGATACAACTACACCTCCCTCACCTGGGCCTCCTACGGCTCCCACTGGCGCAACTTGCGACGCATAACCTCCCTCGAGTTATTGTCGTCCAACCGCCTTCAGATGTTTCACGGTATCCGTGCAGATGAAGTTAGGTCATTGATTTGCCTGCTTTTCCGAGGTTCTAACGGCCGTGAATTTCAGAGTTTCGAGATGAAGTCAACGTTTTTTGAGCTGACACTTAATGTTGTGATGAGGATGATTGCCGGAAAGCGGTACTATGGCGAGCATATGGCGGATTTGGAGCAAGCAAAGCGGTTTAAACAGATCGTGACGGAAAGCTTTGAGCTTAGCGGGGTTACGAATATCGGAGATTTTGTGCCTGTTTTGAAGTATTTGGGAGTAACGGGGCTTGAGAAGAAGTTGGTGATATTGCAGAAGAAAAGGGATGGATTCATGCAAGATTTGATAGAAGAACATAGAAAATTGCAGAGTGGCTCTGTTTCTGAACAGAGGAGGAAGACAATGGTGGAAGTTTTGCTTTCCCTGCAAGAAACTGAACCTGAATATTATACAGATGAGATCATAAGAGGCATCATGCAAGTCATGTTATCGGCAGCGACAGACACCTCATCAGGAACCATGGAATGGGCTCTATCCCTTTTGCTCAACAACCCAGAAGCCCTTGCAAAAGCCCAGGCTGAAATTGACATCCACATTGGACAAAGCTGCAGGCTTGTTGAGGAAACAGACCTTCCCAAGTTACCCTATCTCCAAGGCATCATAAACGAAACCCTCCGAATGTACCCAGCAGGCCCACTGCTAGTCCCCCACGAATCATCAGATGACTGCTCTGTGGGCGGCTTCCACGTCCCACGTGGAACCATGTTGTTGGTGAACATATGGGCCATACAACATCATCCAAAGTTGTGGGCACAACCTGACCAATTTAAGCCAGAGAGGTTCCAAAACCTGCAAGAGGCAAGAGATGGTTTTGTTTGGTTGCCATTTGGGGCGGGGAGGAGGGGCTGCCCCGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

471

Amino Acids

53.94

Weight (kDa)

9.14

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1353
AccB7I CCANNNNNTGG 1 cut(s) 1020
AccII CGCG 1 cut(s) 320
AclI AACGTT 1 cut(s) 596
AcoI YGGCCR 1 cut(s) 562
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 1 cut(s) 476
AcvI CACGTG 1 cut(s) 1250
AfaI GTAC 3 cut(s) 35, 650, 1178
AfiI CCNNNNNNNGG 6 cut(s) 194, 417, 418, 645, 1020, 1351
AflII CTTAAG 1 cut(s) 26
AgsI TTSAA 2 cut(s) 214, 763
AjiI CACGTC 1 cut(s) 1243
AjnI CCWGG 2 cut(s) 416, 1068
AloI GAACNNNNNNTCC 2 cut(s) 59, 91
AluBI AGCT 5 cut(s) 43, 607, 714, 721, 1101
AluI AGCT 5 cut(s) 43, 607, 714, 721, 1101
Alw21I GWGCWC 1 cut(s) 17
Ama87I CYCGRG 2 cut(s) 467, 1406
AoxI GGCC 5 cut(s) 196, 420, 562, 1186, 1278
ApeKI GCWGC 3 cut(s) 989, 1101, 1401
ApoI RAATTY 1 cut(s) 569
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 5 cut(s) 260, 295, 420, 1187, 1278
AsuC2I CCSGG 3 cut(s) 293, 1407, 1408
AsuHPI GGTGA 5 cut(s) 303, 328, 406, 811, 1279
AvaI CYCGRG 2 cut(s) 467, 1406
AvaII GGWCC 2 cut(s) 260, 295
BaeGI GKGCMC 1 cut(s) 1309
BanII GRGCYC 1 cut(s) 1027
BbrPI CACGTG 1 cut(s) 1250
BbsI GAAGAC 1 cut(s) 899
Bbv12I GWGCWC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 324
BbvI GCAGC 3 cut(s) 1001, 1088, 1388
BccI CCATC 4 cut(s) 3, 207, 815, 1355
BceAI ACGGC 3 cut(s) 445, 549, 577
BciT130I CCWGG 2 cut(s) 418, 1070
BciVI GTATCC 2 cut(s) 389, 518
BcnI CCSGG 3 cut(s) 293, 1407, 1408
BfaI CTAG 1 cut(s) 1196
BfmI CTRYAG 1 cut(s) 1102
BfrI CTTAAG 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 1353
BfuI GTATCC 2 cut(s) 389, 518
BisI GCNGC 5 cut(s) 321, 990, 1102, 1234, 1402
BlpI GCTNAGC 1 cut(s) 722
BlsI GCNGC 5 cut(s) 322, 991, 1103, 1235, 1403
Bme1390I CCNGG 5 cut(s) 293, 418, 1070, 1407, 1408
Bme18I GGWCC 2 cut(s) 260, 295
BmeT110I CYCGRG 2 cut(s) 467, 1406
BmgBI CACGTC 1 cut(s) 1243
BmgT120I GGNCC 5 cut(s) 260, 295, 420, 1187, 1278
BmiI GGNNCC 5 cut(s) 286, 433, 1012, 1255, 1337
BmrFI CCNGG 5 cut(s) 293, 418, 1070, 1407, 1408
BmrI ACTGGG 1 cut(s) 182
BmsI GCATC 2 cut(s) 975, 1161
BmuI ACTGGG 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 899
Bpu10I CCTNAGC 1 cut(s) 324
Bpu1102I GCTNAGC 1 cut(s) 722
BpuEI CTTGAG 1 cut(s) 806
BpuMI CCSGG 3 cut(s) 293, 1407, 1408
BsaAI YACGTR 1 cut(s) 1250
BsaJI CCNNGG 9 cut(s) 318, 391, 417, 550, 1014, 1068, 1147, 1405, 1406
BsaXI ACNNNNNCTCC 6 cut(s) 392, 422, 735, 765, 1128, 1158
Bsc4I CCNNNNNNNGG 6 cut(s) 194, 417, 418, 645, 1020, 1351
Bse1I ACTGG 2 cut(s) 188, 443
BseBI CCWGG 2 cut(s) 418, 1070
BseDI CCNNGG 9 cut(s) 318, 391, 417, 550, 1014, 1068, 1147, 1405, 1406
BseGI GGATG 5 cut(s) 14, 636, 826, 1083, 1291
BseLI CCNNNNNNNGG 6 cut(s) 194, 417, 418, 645, 1020, 1351
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 2 cut(s) 188, 443
BseRI GAGGAG 3 cut(s) 290, 901, 1408
BseSI GKGCMC 1 cut(s) 1309
BseX3I CGGCCG 1 cut(s) 562
BseXI GCAGC 3 cut(s) 1001, 1088, 1388
BseYI CCCAGC 1 cut(s) 1180
BsgI GTGCAG 1 cut(s) 534
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 565
BshFI GGCC 5 cut(s) 198, 422, 564, 1188, 1280
BsiEI CGRYCG 1 cut(s) 565
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 2 cut(s) 467, 1406
BsiSI CCGG 3 cut(s) 293, 639, 1407
BslFI GGGAC 2 cut(s) 1184, 1229
BslI CCNNNNNNNGG 6 cut(s) 194, 417, 418, 645, 1020, 1351
BsmFI GGGAC 2 cut(s) 1184, 1229
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 5 cut(s) 198, 422, 564, 1188, 1280
BsoBI CYCGRG 2 cut(s) 467, 1406
Bsp1286I GDGCHC 3 cut(s) 17, 1027, 1309
Bsp143I GATC 2 cut(s) 699, 954
Bsp1720I GCTNAGC 1 cut(s) 722
Bsp19I CCATGG 1 cut(s) 1014
BspANI GGCC 5 cut(s) 198, 422, 564, 1188, 1280
BspCNI CTCAG 1 cut(s) 316
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 5 cut(s) 286, 433, 1012, 1255, 1337
BspMAI CTGCAG 1 cut(s) 1106
BspMI ACCTGC 1 cut(s) 1353
BspTI CTTAAG 1 cut(s) 26
BsrI ACTGG 2 cut(s) 188, 443
BssECI CCNNGG 9 cut(s) 318, 391, 417, 550, 1014, 1068, 1147, 1405, 1406
BssMI GATC 2 cut(s) 699, 954
BssT1I CCWWGG 2 cut(s) 1014, 1147
Bst2UI CCWGG 2 cut(s) 418, 1070
Bst4CI ACNGT 2 cut(s) 315, 506
Bst6I CTCTTC 1 cut(s) 215
BstAFI CTTAAG 1 cut(s) 26
BstBAI YACGTR 1 cut(s) 1250
BstC8I GCNNGC 6 cut(s) 45, 542, 659, 681, 1106, 1186
BstDEI CTNAG 3 cut(s) 324, 369, 722
BstDSI CCRYGG 2 cut(s) 318, 1014
BstENI CCTNNNNNAGG 1 cut(s) 1349
BstF5I GGATG 5 cut(s) 14, 636, 826, 1083, 1291
BstFNI CGCG 1 cut(s) 320
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 2 cut(s) 702, 957
BstMBI GATC 2 cut(s) 699, 954
BstMCI CGRYCG 1 cut(s) 565
BstMWI GCNNNNNNNGC 4 cut(s) 448, 685, 919, 1230
BstNI CCWGG 2 cut(s) 418, 1070
BstSCI CCNGG 5 cut(s) 291, 416, 1068, 1405, 1406
BstSFI CTRYAG 1 cut(s) 1102
BstSLI GKGCMC 1 cut(s) 1309
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 3 cut(s) 1001, 1088, 1388
BstV2I GAAGAC 1 cut(s) 899
BstXI CCANNNNNNTGG 2 cut(s) 1264, 1302
BstZI CGGCCG 1 cut(s) 562
BsuI GTATCC 2 cut(s) 389, 518
BsuRI GGCC 5 cut(s) 198, 422, 564, 1188, 1280
BtgI CCRYGG 2 cut(s) 318, 1014
BtrI CACGTC 1 cut(s) 1243
BtsCI GGATG 5 cut(s) 14, 636, 826, 1083, 1291
BtsI GCAGTG 1 cut(s) 1190
BtsIMutI CAGTG 2 cut(s) 436, 1190
BveI ACCTGC 1 cut(s) 1353
Cac8I GCNNGC 6 cut(s) 45, 542, 659, 681, 1106, 1186
CfoI GCGC 1 cut(s) 444
Cfr13I GGNCC 5 cut(s) 260, 295, 420, 1187, 1278
Cfr42I CCGCGG 1 cut(s) 321
Cfr9I CCCGGG 1 cut(s) 1406
CseI GACGC 1 cut(s) 462
Csp6I GTAC 3 cut(s) 34, 649, 1177
CviAII CATG 6 cut(s) 257, 829, 970, 979, 1015, 1258
CviQI GTAC 3 cut(s) 34, 649, 1177
DdeI CTNAG 3 cut(s) 324, 369, 722
DpnI GATC 2 cut(s) 701, 956
DpnII GATC 2 cut(s) 699, 954
DraI TTTAAA 1 cut(s) 694
EaeI YGGCCR 1 cut(s) 562
EagI CGGCCG 1 cut(s) 562
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
EciI GGCGGA 1 cut(s) 683
EclXI CGGCCG 1 cut(s) 562
Eco130I CCWWGG 2 cut(s) 1014, 1147
Eco24I GRGCYC 1 cut(s) 1027
Eco47I GGWCC 2 cut(s) 260, 295
Eco52I CGGCCG 1 cut(s) 562
Eco57I CTGAAG 1 cut(s) 476
Eco72I CACGTG 1 cut(s) 1250
Eco88I CYCGRG 2 cut(s) 467, 1406
EcoNI CCTNNNNNAGG 1 cut(s) 1349
EcoRII CCWGG 2 cut(s) 416, 1068
EcoT14I CCWWGG 2 cut(s) 1014, 1147
EcoT38I GRGCYC 1 cut(s) 1027
ErhI CCWWGG 2 cut(s) 1014, 1147
FaeI CATG 6 cut(s) 260, 832, 973, 982, 1018, 1261
FalI AAGNNNNNCTT 2 cut(s) 897, 929
FaqI GGGAC 2 cut(s) 1184, 1229
FatI CATG 6 cut(s) 256, 828, 969, 978, 1014, 1257
FauI CCCGC 3 cut(s) 296, 719, 1381
FauNDI CATATG 2 cut(s) 663, 1274
Fnu4HI GCNGC 5 cut(s) 321, 990, 1102, 1234, 1402
FokI GGATG 5 cut(s) 21, 643, 833, 1070, 1278
FriOI GRGCYC 1 cut(s) 1027
Fsp4HI GCNGC 5 cut(s) 321, 990, 1102, 1234, 1402
FspBI CTAG 1 cut(s) 1196
GlaI GCGC 1 cut(s) 443
GluI GCNGC 5 cut(s) 321, 990, 1102, 1234, 1402
GsaI CCCAGC 1 cut(s) 1184
HaeIII GGCC 5 cut(s) 198, 422, 564, 1188, 1280
HapII CCGG 3 cut(s) 293, 639, 1407
HgaI GACGC 1 cut(s) 462
HhaI GCGC 1 cut(s) 444
Hin1II CATG 6 cut(s) 260, 832, 973, 982, 1018, 1261
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 1 cut(s) 594
HindII GTYRAC 1 cut(s) 594
HindIII AAGCTT 1 cut(s) 712
HinfI GANTC 2 cut(s) 825, 1208
HpaII CCGG 3 cut(s) 293, 639, 1407
HphI GGTGA 5 cut(s) 303, 328, 406, 811, 1279
Hpy166II GTNNAC 3 cut(s) 36, 594, 1270
Hpy188I TCNGA 9 cut(s) 278, 395, 495, 551, 576, 743, 880, 1172, 1216
Hpy188III TCNNGA 3 cut(s) 583, 703, 1008
Hpy8I GTNNAC 3 cut(s) 36, 594, 1270
Hpy99I CGWCG 1 cut(s) 456
HpyAV CCTTC 2 cut(s) 500, 1136
HpyCH4III ACNGT 2 cut(s) 315, 506
HpyCH4IV ACGT 3 cut(s) 596, 1242, 1249
HpyF10VI GCNNNNNNNGC 4 cut(s) 448, 685, 919, 1230
HpyF3I CTNAG 3 cut(s) 324, 369, 722
HpySE526I ACGT 3 cut(s) 596, 1242, 1249
Hsp92II CATG 6 cut(s) 260, 832, 973, 982, 1018, 1261
HspAI GCGC 1 cut(s) 442
KspI CCGCGG 1 cut(s) 321
Kzo9I GATC 2 cut(s) 699, 954
LmnI GCTCC 4 cut(s) 12, 290, 437, 676
Lsp1109I GCAGC 3 cut(s) 1001, 1088, 1388
LweI GCATC 2 cut(s) 975, 1161
MaeI CTAG 1 cut(s) 1196
MaeII ACGT 3 cut(s) 596, 1242, 1249
MaeIII GTNAC 4 cut(s) 703, 730, 775, 1134
MalI GATC 2 cut(s) 701, 956
MboI GATC 2 cut(s) 699, 954
MhlI GDGCHC 3 cut(s) 17, 1027, 1309
MluCI AATT 5 cut(s) 147, 569, 857, 1077, 1319
MmeI TCCRAC 2 cut(s) 273, 507
MseI TTAA 5 cut(s) 27, 150, 615, 693, 1323
MslI CAYNNNNRTG 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 320
MspCI CTTAAG 1 cut(s) 26
MspI CCGG 3 cut(s) 293, 639, 1407
MspR9I CCNGG 5 cut(s) 293, 418, 1070, 1407, 1408
MssI GTTTAAAC 1 cut(s) 694
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 2 cut(s) 418, 1070
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 4 cut(s) 448, 685, 919, 1230
NciI CCSGG 3 cut(s) 293, 1407, 1408
NcoI CCATGG 1 cut(s) 1014
NdeI CATATG 2 cut(s) 663, 1274
NdeII GATC 2 cut(s) 699, 954
NlaIII CATG 6 cut(s) 260, 832, 973, 982, 1018, 1261
NlaIV GGNNCC 5 cut(s) 286, 433, 1012, 1255, 1337
NmuCI GTSAC 1 cut(s) 703
PaeR7I CTCGAG 1 cut(s) 467
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 2 cut(s) 825, 1208
PflMI CCANNNNNTGG 1 cut(s) 1020
PkrI GCNGC 5 cut(s) 322, 991, 1103, 1235, 1403
PmaCI CACGTG 1 cut(s) 1250
PmeI GTTTAAAC 1 cut(s) 694
PmlI CACGTG 1 cut(s) 1250
Ppu21I YACGTR 1 cut(s) 1250
Psp1406I AACGTT 1 cut(s) 596
Psp6I CCWGG 2 cut(s) 416, 1068
PspCI CACGTG 1 cut(s) 1250
PspFI CCCAGC 1 cut(s) 1180
PspGI CCWGG 2 cut(s) 416, 1068
PspN4I GGNNCC 5 cut(s) 286, 433, 1012, 1255, 1337
PspPI GGNCC 5 cut(s) 260, 295, 420, 1187, 1278
PspXI VCTCGAGB 1 cut(s) 467
PstI CTGCAG 1 cut(s) 1106
RsaI GTAC 3 cut(s) 35, 650, 1178
RsaNI GTAC 3 cut(s) 34, 649, 1177
RseI CAYNNNNRTG 1 cut(s) 62
SacII CCGCGG 1 cut(s) 321
SaqAI TTAA 5 cut(s) 27, 150, 615, 693, 1323
SatI GCNGC 5 cut(s) 321, 990, 1102, 1234, 1402
Sau3AI GATC 2 cut(s) 699, 954
Sau96I GGNCC 5 cut(s) 260, 295, 420, 1187, 1278
ScrFI CCNGG 5 cut(s) 293, 418, 1070, 1407, 1408
SduI GDGCHC 3 cut(s) 17, 1027, 1309
SfaNI GCATC 2 cut(s) 975, 1161
SfcI CTRYAG 1 cut(s) 1102
Sfr274I CTCGAG 1 cut(s) 467
Sfr303I CCGCGG 1 cut(s) 321
SgrBI CCGCGG 1 cut(s) 321
SinI GGWCC 2 cut(s) 260, 295
SlaI CTCGAG 1 cut(s) 467
SmaI CCCGGG 1 cut(s) 1408
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 3 cut(s) 26, 467, 785
SmoI CTYRAG 3 cut(s) 26, 467, 785
Sse9I AATT 5 cut(s) 147, 569, 857, 1077, 1319
SspI AATATT 1 cut(s) 941
SspMI CTAG 1 cut(s) 1196
StyD4I CCNGG 5 cut(s) 291, 416, 1068, 1405, 1406
StyI CCWWGG 2 cut(s) 1014, 1147
TaaI ACNGT 2 cut(s) 315, 506
TaiI ACGT 3 cut(s) 599, 1245, 1252
TaqI TCGA 2 cut(s) 468, 582
TasI AATT 5 cut(s) 147, 569, 857, 1077, 1319
TatI WGTACW 1 cut(s) 33
TauI GCSGC 2 cut(s) 323, 1236
TfiI GAWTC 2 cut(s) 825, 1208
Tru1I TTAA 5 cut(s) 27, 150, 615, 693, 1323
Tru9I TTAA 5 cut(s) 27, 150, 615, 693, 1323
TscAI CASTG 2 cut(s) 443, 1197
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 3 cut(s) 989, 1101, 1401
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 4 cut(s) 100, 534, 602, 817
TspGWI ACGGA 2 cut(s) 500, 722
TspMI CCCGGG 1 cut(s) 1406
TspRI CASTG 2 cut(s) 443, 1197
Van91I CCANNNNNTGG 1 cut(s) 1020
Vha464I CTTAAG 1 cut(s) 26
VpaK11BI GGWCC 2 cut(s) 260, 295
XagI CCTNNNNNAGG 1 cut(s) 1349
XapI RAATTY 1 cut(s) 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 1247
XhoI CTCGAG 1 cut(s) 467
XmaI CCCGGG 1 cut(s) 1406
XspI CTAG 1 cut(s) 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.