Rroxscaffold_7G00159720

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002267
Physical Location & Seq
Forward (+)
2816872 .. 2818741
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159720.1

Sequence Viewer

Length: 1521 bp
ATGGAAACTTCAACCTACTCCTACATCTTGCTCTTCATCATCTTTCTTTTCCTCAAGAGTTATTTGCAGAAAAATAAGAAGAGATTGCCACCAAGCCCTGCTCTCTCTCTCCCCATAATAGGCCATCTCTATCTTTTTAAGAAACCACTCCACAGAACACTTGCAAAGTTATCCGACAAATATGGTCCAATTGTATACATCAAATTTGGCTCCCGCCCTGTCATTCTTGTATCCTCCCCTTCTGCAGCTGAGGAATGCTTCACCAAGCACGATGTCGCTTTTGCAAACCGTCCACAGCTGCTTGCCGGCAAACACCTTGGGTATAACTATACCACAGTTACCTGGGCTTCTTATGGCTCCCACTGGCGCAACTTGAGACGCATAGCTTCCCTTGAACTATTGTCATCCAACAGCCTTCAGATGTTTCATGGCATACGTGCAGATGAAATCAGATCACTCGTTTGCCAGCTTTTTCGAGGCTGTAATGGTGGTGAGTTTCAGGGTGTAGAGATGAAGTCAAAGTTTTTTCAGCTAACTCTCAATGTTATAATGAGAATGGTTGCTGGGAAAAGATACTATGGGGAAGATATAGCAGATTTGGAGGAAGCTAAAATGTTTCAACAAATTGTTACAGAGGGGTTTCAATTGACTGGGGTTACTAATGTTGGAGATTTCATACCAGTTTTAAACTACATAGGAGTAAGCAAGCTTGAGAAGAAGTTGATAATATTGCATAAGAAGAGGGATAAGTTCCTGCAAGATTTGATCAATCAACATAGAAAAATGCAGAGCGACTCTGCTTTGGAAAAGAGAAGCAAGACCATGGTGGATGTCATGTTGTCACTGCAAGGAACTGAAGCCGAATACTATACTGATGAAATCATAAGGGGCATGATGCATGTCATGTTAACAGCAGGAACAGACACCTCAGCTGGAACCATGGAATGGGCTCTATCACTATTGCTCAACAACCCTAAAGCACTTGCAAAGGCCCAGACTGAAATTGAAAACCACATTGGAGAAGGCAGACTAATTAAGGAGTCAGACCTTGCCAAGCTTCCCTATCTTAGTAGCATCATATATGAGACCCTCCGAATGTACCCAGCAGCCCCACTACTGGCGCCCCATGAGGCATCGGATGACTGCACCGTGGATGGCTTCCATGTTCCACGTGGAACAATGCTGTTGGTGAACGTGTGGGCCATACAGAATGATCCTAAGTTGTGGGCAGAACCTGAACAATTTAAGCCAGAGAGGTTCCAAAATATACAACGAGAAAGAGATGGTTTCAAGTGGTTGCCATTTGGGACAGGAAGAAGAGGCTGTCCTGGTGAAGGCTTAGCAAACCGGATTGTAGGGCTGGCCTTAGGGTCACTGATTCAGTGCTTTGAGTGGGAGAGACCTAGTGAGGAAATGATTGACATGAGTATAGGGACTGGGCTCACCATGCCTAAAGCTCAACCATTACTAGCAAAATGCAGGCCACGACCAACAATGCTGGCCCTACTTTCTCAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

506

Amino Acids

57.27

Weight (kDa)

9.07

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 31 - 486 1.8e-98 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 548
AccB1I GGYRCC 1 cut(s) 1120
AccB7I CCANNNNNTGG 1 cut(s) 945
AccI GTMKAC 1 cut(s) 195
AciI CCGC 1 cut(s) 214
AclWI GGATC 1 cut(s) 1208
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 2 cut(s) 401, 876
AcvI CACGTG 1 cut(s) 1172
AcyI GRCGYC 1 cut(s) 1121
AfaI GTAC 1 cut(s) 1100
AfiI CCNNNNNNNGG 4 cut(s) 119, 945, 1117, 1334
AflIII ACRYGT 1 cut(s) 1194
AgsI TTSAA 6 cut(s) 12, 395, 620, 644, 1007, 1291
AjnI CCWGG 2 cut(s) 341, 1327
AjuI GAANNNNNNNTTGG 2 cut(s) 999, 1031
Alw26I GTCTC 3 cut(s) 370, 1079, 1393
AlwI GGATC 1 cut(s) 1208
AlwNI CAGNNNCTG 1 cut(s) 1235
AoxI GGCC 6 cut(s) 121, 990, 1200, 1362, 1481, 1500
ApeKI GCWGC 3 cut(s) 245, 298, 1106
ApoI RAATTY 1 cut(s) 203
AspLEI GCGC 2 cut(s) 369, 1123
AspS9I GGNCC 4 cut(s) 185, 991, 1200, 1501
AsuHPI GGTGA 5 cut(s) 253, 503, 1201, 1343, 1435
AvaII GGWCC 1 cut(s) 185
AxyI CCTNAGG 1 cut(s) 1366
BanI GGYRCC 1 cut(s) 1120
BanII GRGCYC 2 cut(s) 952, 1443
BbrPI CACGTG 1 cut(s) 1172
BbvCI CCTCAGC 2 cut(s) 249, 928
BbvI GCAGC 3 cut(s) 257, 285, 1118
BccI CCATC 3 cut(s) 132, 1148, 1277
BciT130I CCWGG 2 cut(s) 343, 1329
BciVI GTATCC 1 cut(s) 241
BclI TGATCA 1 cut(s) 765
BcoDI GTCTC 3 cut(s) 370, 1079, 1393
BfaI CTAG 2 cut(s) 1404, 1469
BfmI CTRYAG 1 cut(s) 243
BfoI RGCGCY 1 cut(s) 1124
BfuI GTATCC 1 cut(s) 241
BisI GCNGC 3 cut(s) 246, 299, 1107
BlpI GCTNAGC 1 cut(s) 1339
BlsI GCNGC 3 cut(s) 247, 300, 1108
Bme1390I CCNGG 2 cut(s) 343, 1329
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 4 cut(s) 185, 991, 1200, 1501
BmiI GGNNCC 5 cut(s) 211, 358, 937, 1122, 1259
BmrFI CCNGG 2 cut(s) 343, 1329
BmrI ACTGGG 2 cut(s) 660, 1446
BmsI GCATC 3 cut(s) 885, 1083, 1142
BmuI ACTGGG 2 cut(s) 660, 1446
Bpu10I CCTNAGC 2 cut(s) 249, 928
Bpu1102I GCTNAGC 1 cut(s) 1339
BpuEI CTTGAG 3 cut(s) 38, 394, 731
BsaAI YACGTR 2 cut(s) 437, 1172
BsaHI GRCGYC 1 cut(s) 1121
BsaI GGTCTC 2 cut(s) 1079, 1393
BsaJI CCNNGG 5 cut(s) 316, 342, 822, 939, 1149
BsaWI WCCGGW 1 cut(s) 1347
Bsc4I CCNNNNNNNGG 4 cut(s) 119, 945, 1117, 1334
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 5 cut(s) 368, 655, 680, 1122, 1441
Bse21I CCTNAGG 1 cut(s) 1366
BseBI CCWGG 2 cut(s) 343, 1329
BseDI CCNNGG 5 cut(s) 316, 342, 822, 939, 1149
BseGI GGATG 4 cut(s) 404, 835, 1144, 1159
BseLI CCNNNNNNNGG 4 cut(s) 119, 945, 1117, 1334
BseMII CTCAG 2 cut(s) 240, 942
BseNI ACTGG 5 cut(s) 368, 655, 680, 1122, 1441
BseXI GCAGC 3 cut(s) 257, 285, 1118
BseYI CCCAGC 2 cut(s) 563, 1102
BsgI GTGCAG 2 cut(s) 459, 1129
BshFI GGCC 6 cut(s) 123, 992, 1202, 1364, 1483, 1502
BshNI GGYRCC 1 cut(s) 1120
BsiSI CCGG 2 cut(s) 306, 1348
BslFI GGGAC 2 cut(s) 1321, 1447
BslI CCNNNNNNNGG 4 cut(s) 119, 945, 1117, 1334
BsmAI GTCTC 3 cut(s) 370, 1079, 1393
BsmBI CGTCTC 1 cut(s) 370
BsmFI GGGAC 2 cut(s) 1321, 1447
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 6 cut(s) 123, 992, 1202, 1364, 1483, 1502
Bso31I GGTCTC 2 cut(s) 1079, 1393
Bsp1286I GDGCHC 2 cut(s) 952, 1443
Bsp143I GATC 3 cut(s) 452, 765, 1213
Bsp1720I GCTNAGC 1 cut(s) 1339
Bsp19I CCATGG 2 cut(s) 822, 939
BspACI CCGC 1 cut(s) 214
BspANI GGCC 6 cut(s) 123, 992, 1202, 1364, 1483, 1502
BspCNI CTCAG 2 cut(s) 241, 941
BspLI GGNNCC 5 cut(s) 211, 358, 937, 1122, 1259
BspMAI CTGCAG 1 cut(s) 247
BspPI GGATC 1 cut(s) 1208
BspQI GCTCTTC 1 cut(s) 38
BspT107I GGYRCC 1 cut(s) 1120
BspTNI GGTCTC 2 cut(s) 1079, 1393
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 5 cut(s) 368, 655, 680, 1122, 1441
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 5 cut(s) 316, 342, 822, 939, 1149
BssMI GATC 3 cut(s) 452, 765, 1213
BssNAI GTATAC 1 cut(s) 196
BssNI GRCGYC 1 cut(s) 1121
BssT1I CCWWGG 3 cut(s) 316, 822, 939
Bst1107I GTATAC 1 cut(s) 196
Bst2UI CCWGG 2 cut(s) 343, 1329
Bst4CI ACNGT 3 cut(s) 290, 337, 1150
Bst6I CTCTTC 4 cut(s) 38, 74, 734, 1312
BstACI GRCGYC 1 cut(s) 1121
BstBAI YACGTR 2 cut(s) 437, 1172
BstC8I GCNNGC 7 cut(s) 303, 307, 467, 707, 1362, 1481, 1500
BstDEI CTNAG 6 cut(s) 249, 928, 1067, 1218, 1339, 1366
BstDSI CCRYGG 3 cut(s) 822, 939, 1149
BstENI CCTNNNNNAGG 1 cut(s) 1332
BstF5I GGATG 4 cut(s) 404, 835, 1144, 1159
BstH2I RGCGCY 1 cut(s) 1124
BstHHI GCGC 2 cut(s) 369, 1123
BstKTI GATC 3 cut(s) 455, 768, 1216
BstMAI GTCTC 3 cut(s) 370, 1079, 1393
BstMBI GATC 3 cut(s) 452, 765, 1213
BstMWI GCNNNNNNNGC 1 cut(s) 1447
BstNI CCWGG 2 cut(s) 343, 1329
BstNSI RCATGY 1 cut(s) 902
BstSCI CCNGG 2 cut(s) 341, 1327
BstSFI CTRYAG 1 cut(s) 243
BstV1I GCAGC 3 cut(s) 257, 285, 1118
BstZ17I GTATAC 1 cut(s) 196
Bsu36I CCTNAGG 1 cut(s) 1366
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 6 cut(s) 123, 992, 1202, 1364, 1483, 1502
BtgI CCRYGG 3 cut(s) 822, 939, 1149
BtsCI GGATG 4 cut(s) 404, 835, 1144, 1159
BtsI GCAGTG 1 cut(s) 842
BtsIMutI CAGTG 4 cut(s) 361, 842, 1373, 1388
Cac8I GCNNGC 7 cut(s) 303, 307, 467, 707, 1362, 1481, 1500
CaiI CAGNNNCTG 1 cut(s) 1235
CfoI GCGC 2 cut(s) 369, 1123
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 4 cut(s) 185, 991, 1200, 1501
CseI GACGC 1 cut(s) 387
Csp6I GTAC 1 cut(s) 1099
CviQI GTAC 1 cut(s) 1099
DdeI CTNAG 6 cut(s) 249, 928, 1067, 1218, 1339, 1366
DinI GGCGCC 1 cut(s) 1122
DpnI GATC 3 cut(s) 454, 767, 1215
DpnII GATC 3 cut(s) 452, 765, 1213
DraI TTTAAA 1 cut(s) 687
Eam1104I CTCTTC 4 cut(s) 38, 74, 734, 1312
EarI CTCTTC 4 cut(s) 38, 74, 734, 1312
Eco130I CCWWGG 3 cut(s) 316, 822, 939
Eco24I GRGCYC 2 cut(s) 952, 1443
Eco31I GGTCTC 2 cut(s) 1079, 1393
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 401, 876
Eco72I CACGTG 1 cut(s) 1172
Eco81I CCTNAGG 1 cut(s) 1366
EcoNI CCTNNNNNAGG 1 cut(s) 1332
EcoRII CCWGG 2 cut(s) 341, 1327
EcoT14I CCWWGG 3 cut(s) 316, 822, 939
EcoT22I ATGCAT 1 cut(s) 900
EcoT38I GRGCYC 2 cut(s) 952, 1443
EgeI GGCGCC 1 cut(s) 1122
EheI GGCGCC 1 cut(s) 1122
ErhI CCWWGG 3 cut(s) 316, 822, 939
Esp3I CGTCTC 1 cut(s) 370
FaqI GGGAC 2 cut(s) 1321, 1447
FauI CCCGC 1 cut(s) 221
FbaI TGATCA 1 cut(s) 765
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 3 cut(s) 246, 299, 1107
FokI GGATG 4 cut(s) 391, 842, 1151, 1166
FriOI GRGCYC 2 cut(s) 952, 1443
Fsp4HI GCNGC 3 cut(s) 246, 299, 1107
FspBI CTAG 2 cut(s) 1404, 1469
GlaI GCGC 2 cut(s) 368, 1122
GluI GCNGC 3 cut(s) 246, 299, 1107
GsaI CCCAGC 2 cut(s) 567, 1106
HaeII RGCGCY 1 cut(s) 1124
HaeIII GGCC 6 cut(s) 123, 992, 1202, 1364, 1483, 1502
HapII CCGG 2 cut(s) 306, 1348
HgaI GACGC 1 cut(s) 387
HhaI GCGC 2 cut(s) 369, 1123
Hin1I GRCGYC 1 cut(s) 1121
Hin6I GCGC 2 cut(s) 367, 1121
HinP1I GCGC 2 cut(s) 367, 1121
HincII GTYRAC 1 cut(s) 909
HindII GTYRAC 1 cut(s) 909
HindIII AAGCTT 2 cut(s) 707, 1055
HinfI GANTC 3 cut(s) 794, 1040, 1378
HpaI GTTAAC 1 cut(s) 909
HpaII CCGG 2 cut(s) 306, 1348
HphI GGTGA 5 cut(s) 253, 503, 1201, 1343, 1435
Hpy166II GTNNAC 4 cut(s) 196, 293, 909, 1192
Hpy188I TCNGA 6 cut(s) 175, 420, 452, 1045, 1094, 1138
Hpy188III TCNNGA 1 cut(s) 55
Hpy8I GTNNAC 4 cut(s) 196, 293, 909, 1192
HpyAV CCTTC 4 cut(s) 249, 425, 1016, 1328
HpyCH4III ACNGT 3 cut(s) 290, 337, 1150
HpyCH4IV ACGT 3 cut(s) 436, 1171, 1194
HpyF10VI GCNNNNNNNGC 1 cut(s) 1447
HpyF3I CTNAG 6 cut(s) 249, 928, 1067, 1218, 1339, 1366
HpySE526I ACGT 3 cut(s) 436, 1171, 1194
Hsp92I GRCGYC 1 cut(s) 1121
HspAI GCGC 2 cut(s) 367, 1121
KasI GGCGCC 1 cut(s) 1120
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
Ksp22I TGATCA 1 cut(s) 765
KspAI GTTAAC 1 cut(s) 909
Kzo9I GATC 3 cut(s) 452, 765, 1213
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 2 cut(s) 215, 362
Lsp1109I GCAGC 3 cut(s) 257, 285, 1118
LweI GCATC 3 cut(s) 885, 1083, 1142
MaeI CTAG 2 cut(s) 1404, 1469
MaeII ACGT 3 cut(s) 436, 1171, 1194
MaeIII GTNAC 5 cut(s) 337, 628, 655, 840, 1371
MalI GATC 3 cut(s) 454, 767, 1215
MboI GATC 3 cut(s) 452, 765, 1213
MboII GAAGA 7 cut(s) 25, 91, 596, 727, 751, 1326, 1329
MfeI CAATTG 2 cut(s) 189, 644
MhlI GDGCHC 2 cut(s) 952, 1443
MluCI AATT 7 cut(s) 189, 203, 624, 644, 1002, 1032, 1241
Mly113I GGCGCC 1 cut(s) 1121
MlyI GAGTC 2 cut(s) 788, 1049
MmeI TCCRAC 3 cut(s) 198, 432, 646
Mph1103I ATGCAT 1 cut(s) 900
MroNI GCCGGC 1 cut(s) 305
MseI TTAA 5 cut(s) 138, 686, 908, 1035, 1245
MspA1I CMGCKG 3 cut(s) 248, 298, 932
MspI CCGG 2 cut(s) 306, 1348
MspR9I CCNGG 2 cut(s) 343, 1329
MunI CAATTG 2 cut(s) 189, 644
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 2 cut(s) 343, 1329
MwoI GCNNNNNNNGC 1 cut(s) 1447
NaeI GCCGGC 1 cut(s) 307
NarI GGCGCC 1 cut(s) 1121
NcoI CCATGG 2 cut(s) 822, 939
NdeII GATC 3 cut(s) 452, 765, 1213
NgoMIV GCCGGC 1 cut(s) 305
NlaIV GGNNCC 5 cut(s) 211, 358, 937, 1122, 1259
NmuCI GTSAC 2 cut(s) 840, 1371
NsiI ATGCAT 1 cut(s) 900
NspI RCATGY 1 cut(s) 902
PciSI GCTCTTC 1 cut(s) 38
PctI GAATGC 1 cut(s) 260
PdiI GCCGGC 1 cut(s) 307
PfeI GAWTC 1 cut(s) 1378
PflMI CCANNNNNTGG 1 cut(s) 945
PkrI GCNGC 3 cut(s) 247, 300, 1108
PleI GAGTC 2 cut(s) 788, 1048
PluTI GGCGCC 1 cut(s) 1124
PmaCI CACGTG 1 cut(s) 1172
PmlI CACGTG 1 cut(s) 1172
PpsI GAGTC 2 cut(s) 788, 1048
Ppu21I YACGTR 2 cut(s) 437, 1172
PsiI TTATAA 1 cut(s) 548
Psp6I CCWGG 2 cut(s) 341, 1327
PspCI CACGTG 1 cut(s) 1172
PspFI CCCAGC 2 cut(s) 563, 1102
PspGI CCWGG 2 cut(s) 341, 1327
PspN4I GGNNCC 5 cut(s) 211, 358, 937, 1122, 1259
PspPI GGNCC 4 cut(s) 185, 991, 1200, 1501
PstI CTGCAG 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 1235
PvuII CAGCTG 3 cut(s) 248, 298, 932
RsaI GTAC 1 cut(s) 1100
RsaNI GTAC 1 cut(s) 1099
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 5 cut(s) 138, 686, 908, 1035, 1245
SatI GCNGC 3 cut(s) 246, 299, 1107
Sau3AI GATC 3 cut(s) 452, 765, 1213
Sau96I GGNCC 4 cut(s) 185, 991, 1200, 1501
SchI GAGTC 2 cut(s) 788, 1049
ScrFI CCNGG 2 cut(s) 343, 1329
SduI GDGCHC 2 cut(s) 952, 1443
SfaNI GCATC 3 cut(s) 885, 1083, 1142
SfcI CTRYAG 1 cut(s) 243
SfoI GGCGCC 1 cut(s) 1122
SinI GGWCC 1 cut(s) 185
SmlI CTYRAG 3 cut(s) 53, 373, 710
SmoI CTYRAG 3 cut(s) 53, 373, 710
Sse9I AATT 7 cut(s) 189, 203, 624, 644, 1002, 1032, 1241
SsiI CCGC 1 cut(s) 214
SspDI GGCGCC 1 cut(s) 1120
SspI AATATT 1 cut(s) 729
SspMI CTAG 2 cut(s) 1404, 1469
StyD4I CCNGG 2 cut(s) 341, 1327
StyI CCWWGG 3 cut(s) 316, 822, 939
TaaI ACNGT 3 cut(s) 290, 337, 1150
TaiI ACGT 3 cut(s) 439, 1174, 1197
TaqI TCGA 1 cut(s) 475
TasI AATT 7 cut(s) 189, 203, 624, 644, 1002, 1032, 1241
TfiI GAWTC 1 cut(s) 1378
Tru1I TTAA 5 cut(s) 138, 686, 908, 1035, 1245
Tru9I TTAA 5 cut(s) 138, 686, 908, 1035, 1245
TscAI CASTG 4 cut(s) 368, 849, 1380, 1388
TseFI GTSAC 2 cut(s) 840, 1371
TseI GCWGC 3 cut(s) 245, 298, 1106
Tsp45I GTSAC 2 cut(s) 840, 1371
TspDTI ATGAA 6 cut(s) 25, 416, 459, 527, 664, 891
TspRI CASTG 4 cut(s) 368, 849, 1380, 1388
Van91I CCANNNNNTGG 1 cut(s) 945
VpaK11BI GGWCC 1 cut(s) 185
XagI CCTNNNNNAGG 1 cut(s) 1332
XapI RAATTY 1 cut(s) 203
XceI RCATGY 1 cut(s) 902
XcmI CCANNNNNNNNNTGG 1 cut(s) 1169
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 2 cut(s) 1404, 1469
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.