FvH4_2g33471

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002267
Physical Location & Seq
Forward (+)
25103490 .. 25105898
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33471.t1

Sequence Viewer

Length: 1518 bp
ATGGAAACTTCAATCTCCTACTACCTTTGGCTCTTCATCGTCTTTCTTTTCCTCATCAAGAAGTATTTGCAGAAAACTAACAAAAGACTCCCACCAAGCCCTTCTTTTTCATTGCCCATCATAGGTCATCTCTACCTCTTTAAGAAACCTCTCCACAGAACTCTTGCAAAATTATCTGAAAAATATGGTCCAATCTTATACCTTAAACTTGGATCCCGCCCGGTTCTTGTTGTATCTTCTCCATCTTCTGTTGAGGAATGCTTCACCAAAAACGACATCTCTTTCGCAAACCGCCCACAGCTGCTTGCTGGCAAATACCTTGGATATAACTATACCACGGTCATCTGGGCTCCTTATGGCTCTCACTGGCGCAACTTGAGACGCATAGCTGCCCTTGAGCTGCTGTCATCCAGCCGCCTACAGATGTTTCATGACATACGAGCAGATGAAGTCAGGTCGTTAATTTGCAAGCTTTTTCGATGGTCTAATGGTAGTGAACCGCAGAGTTTAGATATGAAGTCAACGTTTTTCGAGCTTACTCTAAATGTTTTGATGAGAGTGATTGCTGGGAAGAGATACTATGGGGAAGACATCGGAGAGTCCAGTGAAGCTAAGATGTTTGAACAAATTGTGAAAGAAAGTTTTCAATTGAGTGCGCCTAACATTGGAGATTTCTTGCCAGTTTTGAAGTACGTTGGATCTGGTGGACTTGAAAAAAAACTGGTGAAATTGCAAAAGATGAGGGATGAGTTCATGCAATCTCTGATCGAAGAACGCAGAAAAGTGCAGAGTGGCAGTGTTTCTGAACAGAAGAGCAAGACCATGGTGGATGTCTTGCTGTCACTGCAGGCAACTGAACCTGAATATTATACTGATGAAATCATAAAAGGCATGATGCAGGTCATGTTATCAGCAGGAAGTGACACCTCAGCTGGAACTATGGAATGGGCTCTATCACTTCTACTCAACAACCCAGAAGCCCTAGCAAAAGCACAGACAGAAATTGATGAAAAAATTGGGAAAAGCAGGCTAATTGAGGAATCAGATCTTGCTAATCTCCCCTATCTCCATTGCATCATAAGTGAGACCCTCAGAATGTGCTCACCAGCCGCACTTATCCCCCCACATGAGTCATCAGAGGACTGCACCGTGGGAGGCTTCAATGTTCCTCGTGGAACAATGCTGTTGGTGAACGCATGGGCCATACATCATGATCCTAAGTTGTGGGAACATCCTGAACAATTTAAGCCAGAGAGGTTTCAAAATGCACAAGAAAGAGATGTTTTCATGTACTTGCCATTTGGGATAGGAAGGAGAGGCTGTCCTGGGGAAGGGTTAGCCAACCGGATCGTAGGGCTGGCATTAGGGTCAGTCATTCAGTGTTTTAACTGGGAGAGAAATGGTGAAGAGATGGTGGACATGACTGAAGGAACTGGCCTCGCAATGCCTAAAGCTCACCCTTTGCTAGCAAAATGCAGCCCACGCCCAACGATGCTGGCCTTACTTTCTCAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

506

Amino Acids

57.11

Weight (kDa)

8.26

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 31 - 485 5e-99 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 889
AciI CCGC 5 cut(s) 217, 292, 415, 500, 1110
AclI AACGTT 1 cut(s) 524
AclWI GGATC 5 cut(s) 207, 220, 706, 1208, 1355
AcuI CTGAAG 1 cut(s) 1446
AfaI GTAC 2 cut(s) 692, 1292
AfiI CCNNNNNNNGG 3 cut(s) 122, 665, 1331
AgsI TTSAA 7 cut(s) 12, 623, 647, 688, 713, 1162, 1262
AjnI CCWGG 1 cut(s) 1324
AluBI AGCT 8 cut(s) 301, 389, 400, 472, 535, 611, 932, 1454
AluI AGCT 8 cut(s) 301, 389, 400, 472, 535, 611, 932, 1454
Alw21I GWGCWC 1 cut(s) 1103
Alw26I GTCTC 2 cut(s) 373, 1079
AlwI GGATC 5 cut(s) 207, 220, 706, 1208, 1355
AoxI GGCC 3 cut(s) 1200, 1435, 1497
ApeKI GCWGC 4 cut(s) 301, 389, 400, 1476
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
Asp700I GAANNNNTTC 1 cut(s) 642
AspLEI GCGC 2 cut(s) 372, 658
AspS9I GGNCC 2 cut(s) 188, 1200
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 6 cut(s) 256, 736, 1095, 1201, 1415, 1448
AsuNHI GCTAGC 1 cut(s) 1465
AvaII GGWCC 1 cut(s) 188
BamHI GGATCC 1 cut(s) 212
BanII GRGCYC 2 cut(s) 352, 952
BauI CACGAG 1 cut(s) 1170
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 1103
BbvCI CCTCAGC 1 cut(s) 928
BbvI GCAGC 4 cut(s) 288, 376, 387, 1488
BccI CCATC 4 cut(s) 125, 250, 474, 1405
BciT130I CCWGG 1 cut(s) 1326
BcnI CCSGG 1 cut(s) 221
BcoDI GTCTC 2 cut(s) 373, 1079
BfaI CTAG 2 cut(s) 983, 1466
BfmI CTRYAG 2 cut(s) 419, 845
BfuAI ACCTGC 1 cut(s) 889
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 6 cut(s) 302, 390, 401, 415, 1110, 1477
BlsI GCNGC 6 cut(s) 303, 391, 402, 416, 1111, 1478
Bme1390I CCNGG 2 cut(s) 221, 1326
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 188, 1200
BmiI GGNNCC 2 cut(s) 214, 351
BmrFI CCNGG 2 cut(s) 221, 1326
BmrI ACTGGG 1 cut(s) 1399
BmsI GCATC 3 cut(s) 885, 1083, 1482
BmtI GCTAGC 1 cut(s) 1469
BmuI ACTGGG 1 cut(s) 1399
BpiI GAAGAC 1 cut(s) 594
Bpu10I CCTNAGC 1 cut(s) 928
BpuEI CTTGAG 2 cut(s) 397, 416
BpuMI CCSGG 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 1079
BsaJI CCNNGG 5 cut(s) 319, 336, 822, 1149, 1325
BsaWI WCCGGW 1 cut(s) 1344
BsaXI ACNNNNNCTCC 2 cut(s) 1306, 1336
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 665, 1331
Bse1I ACTGG 6 cut(s) 371, 603, 680, 726, 1394, 1438
Bse3DI GCAATG 3 cut(s) 110, 1069, 1449
BseBI CCWGG 1 cut(s) 1326
BseDI CCNNGG 5 cut(s) 319, 336, 822, 1149, 1325
BseGI GGATG 4 cut(s) 407, 751, 835, 1231
BseLI CCNNNNNNNGG 3 cut(s) 122, 665, 1331
BseMI GCAATG 3 cut(s) 110, 1069, 1449
BseMII CTCAG 2 cut(s) 942, 1105
BseNI ACTGG 6 cut(s) 371, 603, 680, 726, 1394, 1438
BseXI GCAGC 4 cut(s) 288, 376, 387, 1488
BseYI CCCAGC 1 cut(s) 566
BsgI GTGCAG 2 cut(s) 806, 1129
BshFI GGCC 3 cut(s) 1202, 1437, 1499
BsiHKAI GWGCWC 1 cut(s) 1103
BsiSI CCGG 2 cut(s) 221, 1345
BslI CCNNNNNNNGG 3 cut(s) 122, 665, 1331
BsmAI GTCTC 2 cut(s) 373, 1079
BsmBI CGTCTC 1 cut(s) 373
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 3 cut(s) 1202, 1437, 1499
Bso31I GGTCTC 1 cut(s) 1079
Bsp1286I GDGCHC 3 cut(s) 352, 952, 1103
Bsp143I GATC 6 cut(s) 212, 698, 765, 1045, 1213, 1347
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 5 cut(s) 217, 292, 415, 500, 1110
BspANI GGCC 3 cut(s) 1202, 1437, 1499
BspCNI CTCAG 2 cut(s) 941, 1104
BspHI TCATGA 2 cut(s) 430, 1210
BspLI GGNNCC 2 cut(s) 214, 351
BspMAI CTGCAG 1 cut(s) 849
BspMI ACCTGC 1 cut(s) 889
BspOI GCTAGC 1 cut(s) 1469
BspPI GGATC 5 cut(s) 207, 220, 706, 1208, 1355
BspQI GCTCTTC 2 cut(s) 38, 806
BspTNI GGTCTC 1 cut(s) 1079
BsrDI GCAATG 3 cut(s) 110, 1069, 1449
BsrI ACTGG 6 cut(s) 371, 603, 680, 726, 1394, 1438
BssECI CCNNGG 5 cut(s) 319, 336, 822, 1149, 1325
BssMI GATC 6 cut(s) 212, 698, 765, 1045, 1213, 1347
BssSI CACGAG 1 cut(s) 1170
BssT1I CCWWGG 2 cut(s) 319, 822
Bst2BI CACGAG 1 cut(s) 1170
Bst2UI CCWGG 1 cut(s) 1326
Bst4CI ACNGT 2 cut(s) 340, 1150
Bst6I CTCTTC 4 cut(s) 38, 566, 806, 1401
BstC8I GCNNGC 8 cut(s) 306, 310, 470, 849, 1028, 1359, 1467, 1497
BstDEI CTNAG 4 cut(s) 612, 928, 1091, 1218
BstDSI CCRYGG 3 cut(s) 336, 822, 1149
BstENI CCTNNNNNAGG 1 cut(s) 1329
BstF5I GGATG 4 cut(s) 407, 751, 835, 1231
BstHHI GCGC 2 cut(s) 372, 658
BstKTI GATC 6 cut(s) 215, 701, 768, 1048, 1216, 1350
BstMAI GTCTC 2 cut(s) 373, 1079
BstMBI GATC 6 cut(s) 212, 698, 765, 1045, 1213, 1347
BstMWI GCNNNNNNNGC 2 cut(s) 844, 1482
BstNI CCWGG 1 cut(s) 1326
BstSCI CCNGG 2 cut(s) 219, 1324
BstSFI CTRYAG 2 cut(s) 419, 845
BstV1I GCAGC 4 cut(s) 288, 376, 387, 1488
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 3 cut(s) 212, 698, 1045
BstYI RGATCY 3 cut(s) 212, 698, 1045
BsuRI GGCC 3 cut(s) 1202, 1437, 1499
BtgI CCRYGG 3 cut(s) 336, 822, 1149
BtsCI GGATG 4 cut(s) 407, 751, 835, 1231
BtsI GCAGTG 2 cut(s) 802, 842
BtsIMutI CAGTG 5 cut(s) 364, 610, 802, 842, 1385
BveI ACCTGC 1 cut(s) 889
Cac8I GCNNGC 8 cut(s) 306, 310, 470, 849, 1028, 1359, 1467, 1497
CciI TCATGA 2 cut(s) 430, 1210
CfoI GCGC 2 cut(s) 372, 658
Cfr13I GGNCC 2 cut(s) 188, 1200
CseI GACGC 1 cut(s) 390
Csp6I GTAC 2 cut(s) 691, 1291
CviQI GTAC 2 cut(s) 691, 1291
DdeI CTNAG 4 cut(s) 612, 928, 1091, 1218
DpnI GATC 6 cut(s) 214, 700, 767, 1047, 1215, 1349
DpnII GATC 6 cut(s) 212, 698, 765, 1045, 1213, 1347
Eam1104I CTCTTC 4 cut(s) 38, 566, 806, 1401
EarI CTCTTC 4 cut(s) 38, 566, 806, 1401
Eco130I CCWWGG 2 cut(s) 319, 822
Eco24I GRGCYC 2 cut(s) 352, 952
Eco31I GGTCTC 1 cut(s) 1079
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 1446
EcoNI CCTNNNNNAGG 1 cut(s) 1329
EcoRII CCWGG 1 cut(s) 1324
EcoT14I CCWWGG 2 cut(s) 319, 822
EcoT38I GRGCYC 2 cut(s) 352, 952
ErhI CCWWGG 2 cut(s) 319, 822
Esp3I CGTCTC 1 cut(s) 373
FalI AAGNNNNNCTT 2 cut(s) 88, 120
FauI CCCGC 1 cut(s) 224
Fnu4HI GCNGC 6 cut(s) 302, 390, 401, 415, 1110, 1477
FokI GGATG 4 cut(s) 394, 758, 842, 1218
FriOI GRGCYC 2 cut(s) 352, 952
Fsp4HI GCNGC 6 cut(s) 302, 390, 401, 415, 1110, 1477
FspBI CTAG 2 cut(s) 983, 1466
GlaI GCGC 2 cut(s) 371, 657
GluI GCNGC 6 cut(s) 302, 390, 401, 415, 1110, 1477
GsaI CCCAGC 1 cut(s) 570
HaeIII GGCC 3 cut(s) 1202, 1437, 1499
HapII CCGG 2 cut(s) 221, 1345
HgaI GACGC 1 cut(s) 390
HhaI GCGC 2 cut(s) 372, 658
Hin6I GCGC 2 cut(s) 370, 656
HinP1I GCGC 2 cut(s) 370, 656
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 4 cut(s) 87, 599, 1040, 1130
HpaII CCGG 2 cut(s) 221, 1345
HphI GGTGA 6 cut(s) 256, 736, 1095, 1201, 1415, 1448
Hpy166II GTNNAC 5 cut(s) 497, 522, 707, 1192, 1417
Hpy188I TCNGA 7 cut(s) 178, 596, 765, 805, 1045, 1094, 1138
Hpy188III TCNNGA 4 cut(s) 58, 431, 1211, 1235
Hpy8I GTNNAC 5 cut(s) 497, 522, 707, 1192, 1417
HpyAV CCTTC 4 cut(s) 111, 1305, 1325, 1421
HpyCH4III ACNGT 2 cut(s) 340, 1150
HpyCH4IV ACGT 2 cut(s) 524, 693
HpyF10VI GCNNNNNNNGC 2 cut(s) 844, 1482
HpyF3I CTNAG 4 cut(s) 612, 928, 1091, 1218
HpySE526I ACGT 2 cut(s) 524, 693
HspAI GCGC 2 cut(s) 370, 656
Kzo9I GATC 6 cut(s) 212, 698, 765, 1045, 1213, 1347
LguI GCTCTTC 2 cut(s) 38, 806
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 4 cut(s) 288, 376, 387, 1488
LweI GCATC 3 cut(s) 885, 1083, 1482
MaeI CTAG 2 cut(s) 983, 1466
MaeII ACGT 2 cut(s) 524, 693
MaeIII GTNAC 2 cut(s) 840, 920
MalI GATC 6 cut(s) 214, 700, 767, 1047, 1215, 1349
MboI GATC 6 cut(s) 212, 698, 765, 1045, 1213, 1347
MboII GAAGA 8 cut(s) 25, 228, 237, 583, 599, 782, 823, 1418
MfeI CAATTG 1 cut(s) 647
MflI RGATCY 3 cut(s) 212, 698, 1045
MhlI GDGCHC 3 cut(s) 352, 952, 1103
MluCI AATT 9 cut(s) 170, 462, 627, 647, 728, 1002, 1014, 1032, 1241
MlyI GAGTC 3 cut(s) 81, 608, 1139
MmeI TCCRAC 1 cut(s) 676
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 5 cut(s) 141, 204, 461, 1245, 1386
MspA1I CMGCKG 2 cut(s) 301, 932
MspI CCGG 2 cut(s) 221, 1345
MspR9I CCNGG 2 cut(s) 221, 1326
MunI CAATTG 1 cut(s) 647
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 1 cut(s) 1326
MwoI GCNNNNNNNGC 2 cut(s) 844, 1482
NciI CCSGG 1 cut(s) 221
NcoI CCATGG 1 cut(s) 822
NdeII GATC 6 cut(s) 212, 698, 765, 1045, 1213, 1347
NheI GCTAGC 1 cut(s) 1465
NlaIV GGNNCC 2 cut(s) 214, 351
NmuCI GTSAC 2 cut(s) 840, 920
PagI TCATGA 2 cut(s) 430, 1210
PciSI GCTCTTC 2 cut(s) 38, 806
PctI GAATGC 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 1 cut(s) 1040
PkrI GCNGC 6 cut(s) 303, 391, 402, 416, 1111, 1478
PleI GAGTC 3 cut(s) 81, 607, 1138
PpsI GAGTC 3 cut(s) 81, 607, 1138
Psp1406I AACGTT 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 1324
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 1 cut(s) 1324
PspN4I GGNNCC 2 cut(s) 214, 351
PspPI GGNCC 2 cut(s) 188, 1200
PstI CTGCAG 1 cut(s) 849
PsuI RGATCY 3 cut(s) 212, 698, 1045
PvuII CAGCTG 2 cut(s) 301, 932
RsaI GTAC 2 cut(s) 692, 1292
RsaNI GTAC 2 cut(s) 691, 1291
SapI GCTCTTC 2 cut(s) 38, 806
SaqAI TTAA 5 cut(s) 141, 204, 461, 1245, 1386
SatI GCNGC 6 cut(s) 302, 390, 401, 415, 1110, 1477
Sau3AI GATC 6 cut(s) 212, 698, 765, 1045, 1213, 1347
Sau96I GGNCC 2 cut(s) 188, 1200
SchI GAGTC 3 cut(s) 81, 608, 1139
ScrFI CCNGG 2 cut(s) 221, 1326
SduI GDGCHC 3 cut(s) 352, 952, 1103
SfaNI GCATC 3 cut(s) 885, 1083, 1482
SfcI CTRYAG 2 cut(s) 419, 845
SinI GGWCC 1 cut(s) 188
SmlI CTYRAG 2 cut(s) 376, 395
SmoI CTYRAG 2 cut(s) 376, 395
Sse9I AATT 9 cut(s) 170, 462, 627, 647, 728, 1002, 1014, 1032, 1241
SsiI CCGC 5 cut(s) 217, 292, 415, 500, 1110
SspI AATATT 1 cut(s) 866
SspMI CTAG 2 cut(s) 983, 1466
StyD4I CCNGG 2 cut(s) 219, 1324
StyI CCWWGG 2 cut(s) 319, 822
TaaI ACNGT 2 cut(s) 340, 1150
TaiI ACGT 2 cut(s) 527, 696
TaqI TCGA 3 cut(s) 478, 531, 768
TasI AATT 9 cut(s) 170, 462, 627, 647, 728, 1002, 1014, 1032, 1241
TatI WGTACW 1 cut(s) 1290
TauI GCSGC 2 cut(s) 417, 1112
TfiI GAWTC 1 cut(s) 1040
Tru1I TTAA 5 cut(s) 141, 204, 461, 1245, 1386
Tru9I TTAA 5 cut(s) 141, 204, 461, 1245, 1386
TscAI CASTG 5 cut(s) 371, 610, 802, 849, 1385
TseFI GTSAC 2 cut(s) 840, 920
TseI GCWGC 4 cut(s) 301, 389, 400, 1476
Tsp45I GTSAC 2 cut(s) 840, 920
TspDTI ATGAA 9 cut(s) 25, 99, 419, 462, 530, 742, 891, 1023, 1276
TspRI CASTG 5 cut(s) 371, 610, 802, 849, 1385
VpaK11BI GGWCC 1 cut(s) 188
XagI CCTNNNNNAGG 1 cut(s) 1329
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 2 cut(s) 983, 1466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.