RchiOBHm_Chr6g0309071

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002267
Physical Location & Seq
Forward (+)
66979187 .. 66981207
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27792

Sequence Viewer

Length: 1590 bp
ATGAAGTACATCAGCTTCAGAGTTGCAGAGATCAACATAGCCATGGAAACCTCATTCCATTACTACATTTTGCTCCTCATTATTTATCTTCTCCTCAAGAACTACTTGCAGAAAAATAACAAAAGACTCCCACCAAGCCCTTCTCTATCTTTCCCTATCATAGGTCATCTCTGCCTCATCAAGAAACCCCTCCACAGAACACTTGCAAAATTAGCCAACAAATATGGATCAATCTTGTACATCAAGTGCGGCTCCCGCCCTGTCGTCATTGTATCTTCTCCTTCTGCAGCTGAGGAATGCTTCACCAAAAATGACATTGCTTTTGCTAACCGCCCCGGGCTGCTTGCCGGAAAACACCTAGGATATAAATACACCACCCTTGTCTGGGCCTCTTATGGCTCTCACTGGCGCAACTTGAGGCGCATAGCTTCCCTTGAGCTATTGTCATCTCACCGCCTTCAGATGTTTCATGGAATACGTGCAGATGAAGTCAGATCATTGATTTTACGCCTTTTCCGAGGTTCCATTGGTGGTGAGTTTTACATTGTGGACATGAAGTCGACGTTTTTTCAGCTGACTCTTAATGTTATCATGAGGATGATTGCTGGAAAAAGATACTATGAGGAAGGCAGGACAGGATTGGAGGAAGCTAAGAGGTTTGAACAGATTGTGGTAGAGACATTTCAATTGAGTGGAGCTACTAACATTGGAGATTTTGTACCAGTTTTGAAATATGTTGGATCAAATGGGCTTGAAAAAAAGTTGTTGATATTGCAAAAGAAGAGGGACAAGTTCATGCAAGATTTGATTGAAGAACATAGAAGAGGGCAGAGTGGCTCTGTTTCTGAACAGAGGAGCAAGACCATGGTGGATGTGTTGCTGTCACTGCAAGAAACTGAACCTGAATACTACACTGATGAAATCATAAGAGGCCTGATGCAAGTGATGTTATCAGCAGGAACAGAAACCTCAGCTGGAACCATGGAATGGGGCTTTTCACTTTTGCTCAACAACCCAGAAGCCCTAGCAAAAGCACAGACAGAAATTGATGTCCAAATTGGGCAAACCAGGCTCATAGAGGAAGCAGACCTTGCCAACCTTCCCTATCTCCATGGCATCATAAACGAGACCCTCAGAATGTACCCAGCAGCCCCACTTCTAGTACCCCATGAGTCATCACAAGAGTGTACACTGGGAGGCTACCATGTCCCACGTGGCACAATGCTGTTGGTGAACATGTGGGCCATACAGGATGATCCCAAATTGTGGGAGCAGCCAGAGCAGTTCAAGCCAGAGAGATTTTTAACTCCACAGAAGGAAGCAAACGGTTTCAAGTTGCTGCCATTTGGGACAGGAAGAAGGGGCTGTCCCGGCGAAGGGTTAGCGACCAGAATCGTAGGGCTGGCCTTAGGGTCACTGATCCAGTGTTTCGAGTGGAAGAGAAATGGTGAAGAAATGGTGGACATGAGCGAAGGGGCTGGCCTCACCGTGCCTAAAGCTCAAGCCTTACTAGCGAAGTGCAGGCCACGCCCGATAATGCTAGCCCTACTTTCACAGCTCTGGTCTTTGTCTAAGAATATCAAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

529

Amino Acids

59.79

Weight (kDa)

9.12

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 45 - 500 1.4e-97 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 987, 1266
AccI GTMKAC 1 cut(s) 560
AciI CCGC 4 cut(s) 249, 256, 331, 454
AclWI GGATC 4 cut(s) 235, 748, 1250, 1414
AcuI CTGAAG 1 cut(s) 443
AcvI CACGTG 1 cut(s) 1214
AfaI GTAC 6 cut(s) 8, 239, 720, 1142, 1164, 1189
AfiI CCNNNNNNNGG 7 cut(s) 161, 384, 385, 987, 1266, 1376, 1377
AflIII ACRYGT 1 cut(s) 1236
AgsI TTSAA 7 cut(s) 662, 686, 730, 755, 812, 1288, 1333
AhdI GACNNNNNGTC 1 cut(s) 556
AjnI CCWGG 1 cut(s) 1067
AleI CACNNNNGTG 1 cut(s) 1183
Alw26I GTCTC 2 cut(s) 671, 1121
AlwI GGATC 4 cut(s) 235, 748, 1250, 1414
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 6 cut(s) 387, 931, 1242, 1404, 1480, 1523
ApeKI GCWGC 5 cut(s) 287, 340, 1148, 1273, 1339
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AspA2I CCTAGG 1 cut(s) 358
AspLEI GCGC 2 cut(s) 411, 423
AspS9I GGNCC 2 cut(s) 387, 1242
AsuC2I CCSGG 3 cut(s) 336, 337, 1371
AsuHPI GGTGA 6 cut(s) 295, 443, 545, 1243, 1460, 1477
AsuNHI GCTAGC 1 cut(s) 1540
AvaI CYCGRG 1 cut(s) 335
AvrII CCTAGG 1 cut(s) 358
AxyI CCTNAGG 1 cut(s) 1408
BarI GAAGNNNNNNTAC 1 cut(s) 31
BbrPI CACGTG 1 cut(s) 1214
BbvCI CCTCAGC 2 cut(s) 291, 970
BbvI GCAGC 5 cut(s) 299, 327, 1160, 1285, 1326
BciT130I CCWGG 1 cut(s) 1069
BcnI CCSGG 3 cut(s) 336, 337, 1371
BcoDI GTCTC 2 cut(s) 671, 1121
BfaI CTAG 5 cut(s) 359, 1025, 1160, 1511, 1541
BfmI CTRYAG 1 cut(s) 285
BisI GCNGC 6 cut(s) 250, 288, 341, 1149, 1274, 1340
BlnI CCTAGG 1 cut(s) 358
BlsI GCNGC 6 cut(s) 251, 289, 342, 1150, 1275, 1341
Bme1390I CCNGG 4 cut(s) 336, 337, 1069, 1371
BmeRI GACNNNNNGTC 1 cut(s) 556
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 2 cut(s) 387, 1242
BmiI GGNNCC 3 cut(s) 253, 523, 979
BmrFI CCNGG 4 cut(s) 336, 337, 1069, 1371
BmrI ACTGGG 1 cut(s) 1202
BmsI GCATC 2 cut(s) 927, 1125
BmtI GCTAGC 1 cut(s) 1544
BmuI ACTGGG 1 cut(s) 1202
Bpu10I CCTNAGC 2 cut(s) 291, 970
BpuEI CTTGAG 4 cut(s) 80, 436, 455, 1485
BpuMI CCSGG 3 cut(s) 336, 337, 1371
BsaAI YACGTR 2 cut(s) 479, 1214
BsaI GGTCTC 1 cut(s) 1121
BsaJI CCNNGG 8 cut(s) 42, 334, 335, 358, 517, 864, 981, 1111
BsaXI ACNNNNNCTCC 2 cut(s) 702, 732
Bsc4I CCNNNNNNNGG 7 cut(s) 161, 384, 385, 987, 1266, 1376, 1377
Bse1I ACTGG 4 cut(s) 410, 722, 1197, 1423
Bse21I CCTNAGG 1 cut(s) 1408
Bse3DI GCAATG 1 cut(s) 315
BseBI CCWGG 1 cut(s) 1069
BseDI CCNNGG 8 cut(s) 42, 334, 335, 358, 517, 864, 981, 1111
BseGI GGATG 3 cut(s) 603, 877, 1258
BseLI CCNNNNNNNGG 7 cut(s) 161, 384, 385, 987, 1266, 1376, 1377
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 3 cut(s) 282, 984, 1147
BseNI ACTGG 4 cut(s) 410, 722, 1197, 1423
BseRI GAGGAG 3 cut(s) 65, 83, 868
BseXI GCAGC 5 cut(s) 299, 327, 1160, 1285, 1326
BseYI CCCAGC 1 cut(s) 1144
BsgI GTGCAG 2 cut(s) 501, 1540
BshFI GGCC 6 cut(s) 389, 933, 1244, 1406, 1482, 1525
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 3 cut(s) 336, 348, 1371
BslFI GGGAC 4 cut(s) 800, 1193, 1353, 1363
BslI CCNNNNNNNGG 7 cut(s) 161, 384, 385, 987, 1266, 1376, 1377
BsmAI GTCTC 2 cut(s) 671, 1121
BsmFI GGGAC 4 cut(s) 800, 1193, 1353, 1363
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 6 cut(s) 389, 933, 1244, 1406, 1482, 1525
Bso31I GGTCTC 1 cut(s) 1121
BsoBI CYCGRG 1 cut(s) 335
Bsp1407I TGTACA 2 cut(s) 237, 1187
Bsp143I GATC 6 cut(s) 30, 227, 494, 740, 1255, 1419
Bsp19I CCATGG 4 cut(s) 42, 864, 981, 1111
BspACI CCGC 4 cut(s) 249, 256, 331, 454
BspANI GGCC 6 cut(s) 389, 933, 1244, 1406, 1482, 1525
BspCNI CTCAG 3 cut(s) 283, 983, 1146
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 3 cut(s) 253, 523, 979
BspMAI CTGCAG 1 cut(s) 289
BspOI GCTAGC 1 cut(s) 1544
BspPI GGATC 4 cut(s) 235, 748, 1250, 1414
BspTNI GGTCTC 1 cut(s) 1121
BsrDI GCAATG 1 cut(s) 315
BsrGI TGTACA 2 cut(s) 237, 1187
BsrI ACTGG 4 cut(s) 410, 722, 1197, 1423
BssECI CCNNGG 8 cut(s) 42, 334, 335, 358, 517, 864, 981, 1111
BssMI GATC 6 cut(s) 30, 227, 494, 740, 1255, 1419
BssT1I CCWWGG 5 cut(s) 42, 358, 864, 981, 1111
Bst2UI CCWGG 1 cut(s) 1069
Bst4CI ACNGT 2 cut(s) 1328, 1489
Bst6I CTCTTC 3 cut(s) 776, 817, 1433
BstAPI GCANNNNNTGC 1 cut(s) 1091
BstAUI TGTACA 2 cut(s) 237, 1187
BstBAI YACGTR 2 cut(s) 479, 1214
BstC8I GCNNGC 5 cut(s) 345, 1404, 1480, 1523, 1542
BstDEI CTNAG 6 cut(s) 291, 651, 970, 1133, 1408, 1572
BstDSI CCRYGG 4 cut(s) 42, 864, 981, 1111
BstENI CCTNNNNNAGG 1 cut(s) 159
BstF5I GGATG 3 cut(s) 603, 877, 1258
BstHHI GCGC 2 cut(s) 411, 423
BstKTI GATC 6 cut(s) 33, 230, 497, 743, 1258, 1422
BstMAI GTCTC 2 cut(s) 671, 1121
BstMBI GATC 6 cut(s) 30, 227, 494, 740, 1255, 1419
BstNI CCWGG 1 cut(s) 1069
BstNSI RCATGY 1 cut(s) 1240
BstSCI CCNGG 4 cut(s) 334, 335, 1067, 1369
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 5 cut(s) 299, 327, 1160, 1285, 1326
Bsu36I CCTNAGG 1 cut(s) 1408
BsuRI GGCC 6 cut(s) 389, 933, 1244, 1406, 1482, 1525
BtgI CCRYGG 4 cut(s) 42, 864, 981, 1111
BtsCI GGATG 3 cut(s) 603, 877, 1258
BtsI GCAGTG 1 cut(s) 884
BtsIMutI CAGTG 6 cut(s) 403, 884, 912, 1190, 1415, 1430
Cac8I GCNNGC 5 cut(s) 345, 1404, 1480, 1523, 1542
CciI TCATGA 1 cut(s) 591
CfoI GCGC 2 cut(s) 411, 423
Cfr13I GGNCC 2 cut(s) 387, 1242
Cfr9I CCCGGG 1 cut(s) 335
Csp6I GTAC 6 cut(s) 7, 238, 719, 1141, 1163, 1188
CviQI GTAC 6 cut(s) 7, 238, 719, 1141, 1163, 1188
DdeI CTNAG 6 cut(s) 291, 651, 970, 1133, 1408, 1572
DpnI GATC 6 cut(s) 32, 229, 496, 742, 1257, 1421
DpnII GATC 6 cut(s) 30, 227, 494, 740, 1255, 1419
DriI GACNNNNNGTC 1 cut(s) 556
Eam1104I CTCTTC 3 cut(s) 776, 817, 1433
Eam1105I GACNNNNNGTC 1 cut(s) 556
EarI CTCTTC 3 cut(s) 776, 817, 1433
Eco130I CCWWGG 5 cut(s) 42, 358, 864, 981, 1111
Eco147I AGGCCT 1 cut(s) 933
Eco31I GGTCTC 1 cut(s) 1121
Eco57I CTGAAG 1 cut(s) 443
Eco72I CACGTG 1 cut(s) 1214
Eco81I CCTNAGG 1 cut(s) 1408
Eco88I CYCGRG 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 1067
EcoT14I CCWWGG 5 cut(s) 42, 358, 864, 981, 1111
ErhI CCWWGG 5 cut(s) 42, 358, 864, 981, 1111
FalI AAGNNNNNCTT 4 cut(s) 89, 121, 1074, 1106
FaqI GGGAC 4 cut(s) 800, 1193, 1353, 1363
FauI CCCGC 1 cut(s) 263
FblI GTMKAC 1 cut(s) 560
Fnu4HI GCNGC 6 cut(s) 250, 288, 341, 1149, 1274, 1340
FokI GGATG 3 cut(s) 610, 884, 1265
Fsp4HI GCNGC 6 cut(s) 250, 288, 341, 1149, 1274, 1340
FspBI CTAG 5 cut(s) 359, 1025, 1160, 1511, 1541
GlaI GCGC 2 cut(s) 410, 422
GluI GCNGC 6 cut(s) 250, 288, 341, 1149, 1274, 1340
GsaI CCCAGC 1 cut(s) 1148
HaeIII GGCC 6 cut(s) 389, 933, 1244, 1406, 1482, 1525
HapII CCGG 3 cut(s) 336, 348, 1371
HhaI GCGC 2 cut(s) 411, 423
Hin6I GCGC 2 cut(s) 409, 421
HinP1I GCGC 2 cut(s) 409, 421
HincII GTYRAC 1 cut(s) 561
HindII GTYRAC 1 cut(s) 561
HinfI GANTC 4 cut(s) 126, 577, 1172, 1392
HpaII CCGG 3 cut(s) 336, 348, 1371
HphI GGTGA 6 cut(s) 295, 443, 545, 1243, 1460, 1477
Hpy166II GTNNAC 6 cut(s) 550, 561, 1188, 1190, 1234, 1462
Hpy188I TCNGA 6 cut(s) 20, 462, 494, 518, 847, 1136
Hpy188III TCNNGA 3 cut(s) 97, 181, 592
Hpy8I GTNNAC 6 cut(s) 550, 561, 1188, 1190, 1234, 1462
Hpy99I CGWCG 1 cut(s) 565
HpyAV CCTTC 9 cut(s) 150, 291, 467, 620, 1109, 1309, 1353, 1370, 1466
HpyCH4III ACNGT 2 cut(s) 1328, 1489
HpyCH4IV ACGT 3 cut(s) 478, 563, 1213
HpyF3I CTNAG 6 cut(s) 291, 651, 970, 1133, 1408, 1572
HpySE526I ACGT 3 cut(s) 478, 563, 1213
HspAI GCGC 2 cut(s) 409, 421
Kzo9I GATC 6 cut(s) 30, 227, 494, 740, 1255, 1419
LmnI GCTCC 5 cut(s) 78, 257, 695, 855, 1270
Lsp1109I GCAGC 5 cut(s) 299, 327, 1160, 1285, 1326
LweI GCATC 2 cut(s) 927, 1125
MaeI CTAG 5 cut(s) 359, 1025, 1160, 1511, 1541
MaeII ACGT 3 cut(s) 478, 563, 1213
MaeIII GTNAC 2 cut(s) 882, 1413
MalI GATC 6 cut(s) 32, 229, 496, 742, 1257, 1421
MboI GATC 6 cut(s) 30, 227, 494, 740, 1255, 1419
MboII GAAGA 8 cut(s) 80, 267, 793, 824, 834, 1368, 1450, 1463
MfeI CAATTG 1 cut(s) 686
MluCI AATT 5 cut(s) 209, 686, 1044, 1056, 1262
MlyI GAGTC 3 cut(s) 120, 571, 1181
MmeI TCCRAC 1 cut(s) 718
MseI TTAA 2 cut(s) 582, 1304
MslI CAYNNNNRTG 3 cut(s) 41, 596, 1183
MspA1I CMGCKG 3 cut(s) 290, 574, 974
MspI CCGG 3 cut(s) 336, 348, 1371
MspR9I CCNGG 4 cut(s) 336, 337, 1069, 1371
MunI CAATTG 1 cut(s) 686
Mva1269I GAATGC 1 cut(s) 302
MvaI CCWGG 1 cut(s) 1069
NciI CCSGG 3 cut(s) 336, 337, 1371
NcoI CCATGG 4 cut(s) 42, 864, 981, 1111
NdeII GATC 6 cut(s) 30, 227, 494, 740, 1255, 1419
NheI GCTAGC 1 cut(s) 1540
NlaIV GGNNCC 3 cut(s) 253, 523, 979
NmuCI GTSAC 2 cut(s) 882, 1413
NspI RCATGY 1 cut(s) 1240
OliI CACNNNNGTG 1 cut(s) 1183
PagI TCATGA 1 cut(s) 591
PceI AGGCCT 1 cut(s) 933
PciI ACATGT 1 cut(s) 1236
PcsI WCGNNNNNNNCGW 1 cut(s) 514
PctI GAATGC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 1392
PflMI CCANNNNNTGG 2 cut(s) 987, 1266
PkrI GCNGC 6 cut(s) 251, 289, 342, 1150, 1275, 1341
PleI GAGTC 3 cut(s) 120, 571, 1180
PmaCI CACGTG 1 cut(s) 1214
PmlI CACGTG 1 cut(s) 1214
PpsI GAGTC 3 cut(s) 120, 571, 1180
Ppu21I YACGTR 2 cut(s) 479, 1214
PscI ACATGT 1 cut(s) 1236
Psp6I CCWGG 1 cut(s) 1067
PspCI CACGTG 1 cut(s) 1214
PspFI CCCAGC 1 cut(s) 1144
PspGI CCWGG 1 cut(s) 1067
PspN4I GGNNCC 3 cut(s) 253, 523, 979
PspPI GGNCC 2 cut(s) 387, 1242
PstI CTGCAG 1 cut(s) 289
PvuII CAGCTG 3 cut(s) 290, 574, 974
RsaI GTAC 6 cut(s) 8, 239, 720, 1142, 1164, 1189
RsaNI GTAC 6 cut(s) 7, 238, 719, 1141, 1163, 1188
RseI CAYNNNNRTG 3 cut(s) 41, 596, 1183
SalI GTCGAC 1 cut(s) 559
SaqAI TTAA 2 cut(s) 582, 1304
SatI GCNGC 6 cut(s) 250, 288, 341, 1149, 1274, 1340
Sau3AI GATC 6 cut(s) 30, 227, 494, 740, 1255, 1419
Sau96I GGNCC 2 cut(s) 387, 1242
SchI GAGTC 3 cut(s) 120, 571, 1181
ScrFI CCNGG 4 cut(s) 336, 337, 1069, 1371
SfaNI GCATC 2 cut(s) 927, 1125
SfcI CTRYAG 1 cut(s) 285
SmaI CCCGGG 1 cut(s) 337
SmiMI CAYNNNNRTG 3 cut(s) 41, 596, 1183
SmlI CTYRAG 4 cut(s) 95, 415, 434, 1500
SmoI CTYRAG 4 cut(s) 95, 415, 434, 1500
Sse9I AATT 5 cut(s) 209, 686, 1044, 1056, 1262
SseBI AGGCCT 1 cut(s) 933
SsiI CCGC 4 cut(s) 249, 256, 331, 454
SspMI CTAG 5 cut(s) 359, 1025, 1160, 1511, 1541
StuI AGGCCT 1 cut(s) 933
StyD4I CCNGG 4 cut(s) 334, 335, 1067, 1369
StyI CCWWGG 5 cut(s) 42, 358, 864, 981, 1111
TaaI ACNGT 2 cut(s) 1328, 1489
TaiI ACGT 3 cut(s) 481, 566, 1216
TaqI TCGA 2 cut(s) 560, 1431
TasI AATT 5 cut(s) 209, 686, 1044, 1056, 1262
TatI WGTACW 3 cut(s) 6, 237, 1187
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 1 cut(s) 1392
Tru1I TTAA 2 cut(s) 582, 1304
Tru9I TTAA 2 cut(s) 582, 1304
TscAI CASTG 6 cut(s) 410, 891, 919, 1197, 1422, 1430
TseFI GTSAC 2 cut(s) 882, 1413
TseI GCWGC 5 cut(s) 287, 340, 1148, 1273, 1339
Tsp45I GTSAC 2 cut(s) 882, 1413
TspDTI ATGAA 6 cut(s) 17, 458, 501, 569, 784, 933
TspMI CCCGGG 1 cut(s) 335
TspRI CASTG 6 cut(s) 410, 891, 919, 1197, 1422, 1430
Van91I CCANNNNNTGG 2 cut(s) 987, 1266
XagI CCTNNNNNAGG 1 cut(s) 159
XceI RCATGY 1 cut(s) 1240
XcmI CCANNNNNNNNNTGG 1 cut(s) 1211
XmaI CCCGGG 1 cut(s) 335
XmaJI CCTAGG 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 560
XspI CTAG 5 cut(s) 359, 1025, 1160, 1511, 1541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.