Rh6DG482400

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002267
Physical Location & Seq
Reverse (-)
64603920 .. 64604716
797 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG482400.1

Sequence Viewer

Length: 627 bp
ATGCAAAACATGTTAACAGCAGGAACAGACACCTCGGCTGGAACCATGGAATGGGCTCTATCACTATTGCTCAACAACCCAGAAGCCCTTGCAAAGGAACAGACAGAAATTGATAACAAAATTGGGCAAAGCAGGCTAATTGAGGAATCAGACCTTGCTAATCTCCCCTATCTCCATTGCATCATAAACGAGACCCTCAGAATGTACCCAGCAGCCGCACTTATTCCCCCACACGAGTCATCAGAGGACTGCACCGTGGGAGGCTTCAATGTTCCTCGTGGAACAATGCTGTTGGTGAACGCATGGGCCATACATCATGATCCTAAGTTGTGGGAAGAGCCTGAACAATTTAAGCCAGAGAGGTTCCAAAATGCACAAGAAGAAAGAGATGTTTTCATGTACTTGCCATTTGGGACAGGAAGAAGAGGCTGTCCTGGGGAAGGGTTAGCCAACCGGATGGTAGGGCTGGCCTTAGGGTCAGTCATTCAGTGTTTTGAGTGGGAGAGAAGTGGTGAGGAGATGGTGGACATGACTGAAGGGACTGGCATCTCAATGCCTAAAGCTCACCCTTTGCTAGCAAAATGCAGGCCACGCCCAACGATGCTGGCCTTACTTTCTCAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

208

Amino Acids

23.15

Weight (kDa)

4.9

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 188 8.5e-65 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 51
AciI CCGC 1 cut(s) 216
AclWI GGATC 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 555
AfaI GTAC 2 cut(s) 206, 401
AfiI CCNNNNNNNGG 3 cut(s) 51, 94, 440
AflIII ACRYGT 1 cut(s) 9
AgsI TTSAA 1 cut(s) 268
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 1 cut(s) 563
AluI AGCT 1 cut(s) 563
Alw26I GTCTC 1 cut(s) 185
AlwI GGATC 1 cut(s) 314
AoxI GGCC 4 cut(s) 306, 468, 587, 606
ApeKI GCWGC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 306
AsuHPI GGTGA 3 cut(s) 307, 524, 557
AsuNHI GCTAGC 1 cut(s) 574
AxyI CCTNAGG 1 cut(s) 472
BanII GRGCYC 1 cut(s) 58
BauI CACGAG 2 cut(s) 233, 276
BbvI GCAGC 1 cut(s) 224
BccI CCATC 2 cut(s) 451, 514
BciT130I CCWGG 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 185
BfaI CTAG 1 cut(s) 575
BisI GCNGC 2 cut(s) 213, 216
BlsI GCNGC 2 cut(s) 214, 217
Bme1390I CCNGG 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 306
BmiI GGNNCC 2 cut(s) 43, 365
BmrFI CCNGG 1 cut(s) 435
BmsI GCATC 3 cut(s) 189, 555, 591
BmtI GCTAGC 1 cut(s) 578
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 4 cut(s) 33, 45, 255, 434
BsaWI WCCGGW 1 cut(s) 453
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 94, 440
Bse1I ACTGG 1 cut(s) 547
Bse21I CCTNAGG 1 cut(s) 472
Bse3DI GCAATG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 4 cut(s) 33, 45, 255, 434
BseGI GGATG 1 cut(s) 462
BseLI CCNNNNNNNGG 3 cut(s) 51, 94, 440
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 211
BseNI ACTGG 1 cut(s) 547
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 1 cut(s) 224
BseYI CCCAGC 1 cut(s) 208
BsgI GTGCAG 1 cut(s) 235
BshFI GGCC 4 cut(s) 308, 470, 589, 608
BsiSI CCGG 1 cut(s) 454
BslFI GGGAC 2 cut(s) 427, 553
BslI CCNNNNNNNGG 3 cut(s) 51, 94, 440
BsmAI GTCTC 1 cut(s) 185
BsmFI GGGAC 2 cut(s) 427, 553
BsnI GGCC 4 cut(s) 308, 470, 589, 608
Bso31I GGTCTC 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 58
Bsp143I GATC 1 cut(s) 319
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 1 cut(s) 216
BspANI GGCC 4 cut(s) 308, 470, 589, 608
BspCNI CTCAG 1 cut(s) 210
BspHI TCATGA 1 cut(s) 316
BspLI GGNNCC 2 cut(s) 43, 365
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 1 cut(s) 314
BspQI GCTCTTC 1 cut(s) 330
BspTNI GGTCTC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 175
BsrI ACTGG 1 cut(s) 547
BssECI CCNNGG 4 cut(s) 33, 45, 255, 434
BssMI GATC 1 cut(s) 319
BssSI CACGAG 2 cut(s) 233, 276
BssT1I CCWWGG 1 cut(s) 45
Bst2BI CACGAG 2 cut(s) 233, 276
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 2 cut(s) 330, 418
BstC8I GCNNGC 5 cut(s) 134, 468, 576, 587, 606
BstDEI CTNAG 3 cut(s) 197, 324, 472
BstDSI CCRYGG 2 cut(s) 45, 255
BstENI CCTNNNNNAGG 2 cut(s) 92, 438
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 1 cut(s) 322
BstMAI GTCTC 1 cut(s) 185
BstMBI GATC 1 cut(s) 319
BstMWI GCNNNNNNNGC 2 cut(s) 133, 591
BstNI CCWGG 1 cut(s) 435
BstNSI RCATGY 1 cut(s) 13
BstSCI CCNGG 1 cut(s) 433
BstV1I GCAGC 1 cut(s) 224
BstXI CCANNNNNNTGG 1 cut(s) 457
Bsu36I CCTNAGG 1 cut(s) 472
BsuRI GGCC 4 cut(s) 308, 470, 589, 608
BtgI CCRYGG 2 cut(s) 45, 255
BtsCI GGATG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 494
Cac8I GCNNGC 5 cut(s) 134, 468, 576, 587, 606
CciI TCATGA 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 306
Csp6I GTAC 2 cut(s) 205, 400
CviAII CATG 6 cut(s) 10, 46, 303, 317, 397, 529
CviQI GTAC 2 cut(s) 205, 400
DdeI CTNAG 3 cut(s) 197, 324, 472
DpnI GATC 1 cut(s) 321
DpnII GATC 1 cut(s) 319
Eam1104I CTCTTC 2 cut(s) 330, 418
EarI CTCTTC 2 cut(s) 330, 418
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 58
Eco31I GGTCTC 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 555
Eco81I CCTNAGG 1 cut(s) 472
EcoNI CCTNNNNNAGG 2 cut(s) 92, 438
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 6 cut(s) 13, 49, 306, 320, 400, 532
FaiI YATR 8 cut(s) 11, 47, 185, 304, 311, 318, 398, 530
FaqI GGGAC 2 cut(s) 427, 553
FatI CATG 6 cut(s) 9, 45, 302, 316, 396, 528
Fnu4HI GCNGC 2 cut(s) 213, 216
FokI GGATG 1 cut(s) 469
FriOI GRGCYC 1 cut(s) 58
Fsp4HI GCNGC 2 cut(s) 213, 216
FspBI CTAG 1 cut(s) 575
GluI GCNGC 2 cut(s) 213, 216
GsaI CCCAGC 1 cut(s) 212
HaeIII GGCC 4 cut(s) 308, 470, 589, 608
HapII CCGG 1 cut(s) 454
Hin1II CATG 6 cut(s) 13, 49, 306, 320, 400, 532
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 2 cut(s) 146, 236
HpaI GTTAAC 1 cut(s) 15
HpaII CCGG 1 cut(s) 454
HphI GGTGA 3 cut(s) 307, 524, 557
Hpy166II GTNNAC 3 cut(s) 15, 298, 526
Hpy188I TCNGA 3 cut(s) 151, 200, 244
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 3 cut(s) 15, 298, 526
HpyAV CCTTC 2 cut(s) 434, 530
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4V TGCA 6 cut(s) 4, 92, 180, 252, 374, 585
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 591
HpyF3I CTNAG 3 cut(s) 197, 324, 472
Hsp92II CATG 6 cut(s) 13, 49, 306, 320, 400, 532
KspAI GTTAAC 1 cut(s) 15
Kzo9I GATC 1 cut(s) 319
LguI GCTCTTC 1 cut(s) 330
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 3 cut(s) 189, 555, 591
MaeI CTAG 1 cut(s) 575
MalI GATC 1 cut(s) 321
MboI GATC 1 cut(s) 319
MboII GAAGA 4 cut(s) 347, 392, 432, 435
MhlI GDGCHC 1 cut(s) 58
MluCI AATT 4 cut(s) 108, 120, 138, 347
MlyI GAGTC 1 cut(s) 245
MnlI CCTC 9 cut(s) 43, 136, 206, 238, 254, 285, 354, 419, 508
MseI TTAA 2 cut(s) 14, 351
MslI CAYNNNNRTG 1 cut(s) 551
MspI CCGG 1 cut(s) 454
MspR9I CCNGG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 2 cut(s) 133, 591
NcoI CCATGG 1 cut(s) 45
NdeII GATC 1 cut(s) 319
NheI GCTAGC 1 cut(s) 574
NlaIII CATG 6 cut(s) 13, 49, 306, 320, 400, 532
NlaIV GGNNCC 2 cut(s) 43, 365
NmeAIII GCCGAG 1 cut(s) 14
NspI RCATGY 1 cut(s) 13
PagI TCATGA 1 cut(s) 316
PciI ACATGT 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 330
PfeI GAWTC 1 cut(s) 146
PflMI CCANNNNNTGG 1 cut(s) 51
PkrI GCNGC 2 cut(s) 214, 217
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
PscI ACATGT 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 433
PspFI CCCAGC 1 cut(s) 208
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 43, 365
PspPI GGNCC 1 cut(s) 306
RsaI GTAC 2 cut(s) 206, 401
RsaNI GTAC 2 cut(s) 205, 400
RseI CAYNNNNRTG 1 cut(s) 551
SapI GCTCTTC 1 cut(s) 330
SaqAI TTAA 2 cut(s) 14, 351
SatI GCNGC 2 cut(s) 213, 216
Sau3AI GATC 1 cut(s) 319
Sau96I GGNCC 1 cut(s) 306
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 1 cut(s) 435
SduI GDGCHC 1 cut(s) 58
SetI ASST 4 cut(s) 35, 156, 365, 565
SfaNI GCATC 3 cut(s) 189, 555, 591
SmiMI CAYNNNNRTG 1 cut(s) 551
Sse9I AATT 4 cut(s) 108, 120, 138, 347
SsiI CCGC 1 cut(s) 216
SspMI CTAG 1 cut(s) 575
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 256
TasI AATT 4 cut(s) 108, 120, 138, 347
TatI WGTACW 1 cut(s) 399
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 2 cut(s) 14, 351
Tru9I TTAA 2 cut(s) 14, 351
TscAI CASTG 1 cut(s) 494
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 385
TspRI CASTG 1 cut(s) 494
Van91I CCANNNNNTGG 1 cut(s) 51
XagI CCTNNNNNAGG 2 cut(s) 92, 438
XceI RCATGY 1 cut(s) 13
XspI CTAG 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.