MD15G1074600.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002267
Physical Location & Seq
Forward (+)
5082653 .. 5083509
857 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1074600.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGTGGACGTCTTGCTTGCCCTGCAAGAAACTGAACCGGAATATTATACTGATGAGATCATAAGAGGCATGCTGCAAGTCATGTTAGCAGCAGGGACAGACACCTCATCTGGAACCATGGAATGGGCTCTATCACTTTTGCTCAACAACCCAGAAGCCCTAGCAAAAGCTGAGACTGAAATGGATATCCACATTGGGCAAAGCAGGCTACTTGAGGAGTCCGACCTCGCCAAGCTTCCGTATCTCCACGGCATCATCAATGAAACCCTCCGGATGTACCCAGCAGGCCCATTGCTTGCCCCACACGATTCATCGAGGATTGCACTGTGGGGGGCTTCCACGTTCCACGTGGCACGATGTTGCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

123

Amino Acids

13.54

Weight (kDa)

4.58

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 104 1.3e-25 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 12
AccB7I CCANNNNNTGG 1 cut(s) 123
AccIII TCCGGA 1 cut(s) 270
AcvI CACGTG 1 cut(s) 349
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 1 cut(s) 123
AluBI AGCT 2 cut(s) 170, 235
AluI AGCT 2 cut(s) 170, 235
Alw26I GTCTC 1 cut(s) 167
Aor13HI TCCGGA 1 cut(s) 270
AoxI GGCC 1 cut(s) 286
ApeKI GCWGC 2 cut(s) 73, 89
AspS9I GGNCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 130
BbrPI CACGTG 1 cut(s) 349
BbvI GCAGC 2 cut(s) 60, 101
BceAI ACGGC 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 167
BfaI CTAG 1 cut(s) 161
BisI GCNGC 2 cut(s) 74, 90
BlsI GCNGC 2 cut(s) 75, 91
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 115
BmsI GCATC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 233
BsaAI YACGTR 1 cut(s) 349
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 2 cut(s) 117, 247
BsaWI WCCGGW 2 cut(s) 37, 270
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse3DI GCAATG 1 cut(s) 290
BseAI TCCGGA 1 cut(s) 270
BseDI CCNNGG 2 cut(s) 117, 247
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMI GCAATG 1 cut(s) 290
BseMII CTCAG 1 cut(s) 162
BseRI GAGGAG 1 cut(s) 230
BseXI GCAGC 2 cut(s) 60, 101
BseYI CCCAGC 1 cut(s) 280
BshFI GGCC 1 cut(s) 288
BsiSI CCGG 2 cut(s) 38, 271
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 109
BsnI GGCC 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 130
Bsp13I TCCGGA 1 cut(s) 270
Bsp143I GATC 1 cut(s) 57
Bsp19I CCATGG 1 cut(s) 117
BspANI GGCC 1 cut(s) 288
BspCNI CTCAG 1 cut(s) 163
BspEI TCCGGA 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 115
BsrDI GCAATG 1 cut(s) 290
BssECI CCNNGG 2 cut(s) 117, 247
BssMI GATC 1 cut(s) 57
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 327
BstACI GRCGYC 1 cut(s) 9
BstBAI YACGTR 1 cut(s) 349
BstC8I GCNNGC 5 cut(s) 18, 71, 206, 286, 297
BstDEI CTNAG 1 cut(s) 171
BstDSI CCRYGG 2 cut(s) 117, 247
BstF5I GGATG 1 cut(s) 279
BstKTI GATC 1 cut(s) 60
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 1 cut(s) 57
BstMWI GCNNNNNNNGC 2 cut(s) 22, 205
BstNSI RCATGY 1 cut(s) 73
BstV1I GCAGC 2 cut(s) 60, 101
BsuRI GGCC 1 cut(s) 288
BtgI CCRYGG 2 cut(s) 117, 247
BtsCI GGATG 1 cut(s) 279
BtsIMutI CAGTG 1 cut(s) 323
Cac8I GCNNGC 5 cut(s) 18, 71, 206, 286, 297
Cfr13I GGNCC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 277
CviAII CATG 3 cut(s) 70, 82, 118
CviJI RGCY 7 cut(s) 128, 158, 170, 208, 235, 288, 335
CviKI_1 RGCY 7 cut(s) 128, 158, 170, 208, 235, 288, 335
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 1 cut(s) 171
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 130
Eco32I GATATC 1 cut(s) 187
Eco72I CACGTG 1 cut(s) 349
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 117
EcoT38I GRGCYC 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 3 cut(s) 73, 85, 121
FaiI YATR 5 cut(s) 48, 62, 71, 83, 119
FaqI GGGAC 1 cut(s) 109
FatI CATG 3 cut(s) 69, 81, 117
Fnu4HI GCNGC 2 cut(s) 74, 90
FokI GGATG 1 cut(s) 286
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 2 cut(s) 74, 90
FspBI CTAG 1 cut(s) 161
GluI GCNGC 2 cut(s) 74, 90
GsaI CCCAGC 1 cut(s) 284
HaeIII GGCC 1 cut(s) 288
HapII CCGG 2 cut(s) 38, 271
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 3 cut(s) 73, 85, 121
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 2 cut(s) 218, 308
HpaII CCGG 2 cut(s) 38, 271
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 2 cut(s) 111, 271
Hpy8I GTNNAC 1 cut(s) 7
HpyCH4III ACNGT 1 cut(s) 327
HpyCH4IV ACGT 3 cut(s) 9, 341, 348
HpyCH4V TGCA 3 cut(s) 25, 76, 323
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 205
HpyF3I CTNAG 1 cut(s) 171
HpySE526I ACGT 3 cut(s) 9, 341, 348
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 3 cut(s) 73, 85, 121
Kpn2I TCCGGA 1 cut(s) 270
Kzo9I GATC 1 cut(s) 57
Lsp1109I GCAGC 2 cut(s) 60, 101
LweI GCATC 1 cut(s) 261
MaeI CTAG 1 cut(s) 161
MaeII ACGT 3 cut(s) 9, 341, 348
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MhlI GDGCHC 1 cut(s) 130
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 6 cut(s) 59, 115, 208, 236, 278, 309
MroI TCCGGA 1 cut(s) 270
MspI CCGG 2 cut(s) 38, 271
MwoI GCNNNNNNNGC 2 cut(s) 22, 205
NcoI CCATGG 1 cut(s) 117
NdeII GATC 1 cut(s) 57
NlaIII CATG 3 cut(s) 73, 85, 121
NlaIV GGNNCC 1 cut(s) 115
NspI RCATGY 1 cut(s) 73
PaeI GCATGC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 308
PflMI CCANNNNNTGG 1 cut(s) 123
PkrI GCNGC 2 cut(s) 75, 91
PleI GAGTC 1 cut(s) 226
PmaCI CACGTG 1 cut(s) 349
PmlI CACGTG 1 cut(s) 349
PpsI GAGTC 1 cut(s) 226
Ppu21I YACGTR 1 cut(s) 349
PspCI CACGTG 1 cut(s) 349
PspFI CCCAGC 1 cut(s) 280
PspN4I GGNNCC 1 cut(s) 115
PspPI GGNCC 1 cut(s) 287
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SatI GCNGC 2 cut(s) 74, 90
Sau3AI GATC 1 cut(s) 57
Sau96I GGNCC 1 cut(s) 287
SchI GAGTC 1 cut(s) 227
SduI GDGCHC 1 cut(s) 130
SetI ASST 7 cut(s) 12, 107, 172, 228, 237, 344, 351
SfaNI GCATC 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SphI GCATGC 1 cut(s) 73
SspI AATATT 1 cut(s) 44
SspMI CTAG 1 cut(s) 161
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 1 cut(s) 327
TaiI ACGT 3 cut(s) 12, 344, 351
TaqI TCGA 1 cut(s) 314
TfiI GAWTC 1 cut(s) 308
TscAI CASTG 1 cut(s) 330
TseI GCWGC 2 cut(s) 73, 89
TspDTI ATGAA 2 cut(s) 276, 300
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 1 cut(s) 330
Van91I CCANNNNNTGG 1 cut(s) 123
XceI RCATGY 1 cut(s) 73
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XspI CTAG 1 cut(s) 161
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.