Rroxscaffold_7G00159730

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002267
Physical Location & Seq
Forward (+)
2819452 .. 2821278
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159730.1

Sequence Viewer

Length: 1569 bp
ATGGAAACTTCAATCTCCTACTACCTTTGGCTCTTCATTGTCTTTCTTTTCCTCCTCAAGAACTATTTGCAGAAAACTAACAAAAGACTCCCACCAAGCCCTTCTCTTTCCTTTCCCATCATAGGTCATCTCTACCTCTTTAAGAAACCTCTCCACAGAACACTTGCAAAACTAACCGACAAATATGGTCCAATCTTATACCTTCAATTTGGATCCCGCACGGTTGTTGTTGTATCCTCTCCTTCTGCAGTTGAGGAATGCTTCACCAAAAACGATGTCACTTTCGCAAACCGTCCAAAGCTGCTTGCCGGCAAGCACTTTGGATGTAACTATACTACGATTGTCTGGGCTCCCTATGGCTCTCACTGGCGCAACTTGAGACGCATAGCTTCCCTTGAGCTATTGTCATCCAGCCGACTTCAGATGTTTCATGGCATACGTGCAGATGAAGTTAGGTCACTAATTTGCAAGCTTTTTCGAGGGTCTAAAGGTGGTGAACTGGGGAGTTTAGATATGAAGTCAACGTTTTTCGAGCTTACTCTTAATGTTTTGATGAGGGTGATTGCTGGGAAGAGATACTATGGGGAAGATATCGCAGAGTCCAGCGAAGCAAAGAGTTTTGAACAAATTGTGAAAGAGACTTTTCAATTGAGTGGGGCTAACAATATTGGAGATTTCTTGCCAGTTTTAGATTACTTTGGATCAGGTGGCCTTGAGAAGAAGTTGGTGAAATTGCAACAGAAGAGGGATACGTTCATGCAATATTTGATCGAAGAACATAGAAAAGTGCAGAGTGGCTCAGTTTCTGAACCGAAGAGCAAGACCATGGTGGACGTCTTGTTGTCGCTGCAAGCAACTGAACCTGAATATTATACTGATGAAATCATAAGAGGCATGATGCAAGTAAGCTCTCTCAACCAGAATCTGTCAAATCCAAGAACAACTGCAAACATGTTAACAGCAGGAACAGACACCTCAGCTGGGACCATGGAATGGGCTCTATCACTATTGCTCAACAACCCAGAAGCCCTTGCAAAGGCACAGACAGAAATTGATAACAAAATTGGGCAAAGCAGGCTAATTGAGGAATCAGACCTTGCTAATCTCCCCTATCTCCGCTGCATCGTAAATGAGACCCTCAGAATGTACCCAGCAGCCGCACTTCTTCCCCCACATGAGTCATCAGAGGACTGCACCGTGGGAGGCTTCAACGTTCCTCGTGGAACAATGTTGTTGGTGAACGCATGGGCCATACATCATGATCCTAAGTTGTGGGAAGAGCCTGAACAATTTAAGCCAGAGAGGTTCCAAAATGCACAAGAAGAAAGAGATGTTTTCATGTACTTGCCATTTGGGACAGGAAGGAGAGGCTGTCCTGGGGAAGGGTTAGCAAACCGGATCGTAGGGCTGGCCTTAGGGTCAGTCATTCAGTGTTTTGAATGGGGGAGAAGTGGTGAGGAGATGGTGGACATGAATGAAGGGACTGGCCTCACAATGCCTAAAGCTCACCCTTTGCTAGCAAAATGCAGGCCACGCCCAACAATGCTGGCCTTCCTTTCTCAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

522

Amino Acids

58.78

Weight (kDa)

8.27

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 32 - 502 9.4e-96 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 837
AccB7I CCANNNNNTGG 1 cut(s) 993
AciI CCGC 3 cut(s) 217, 1117, 1158
AclI AACGTT 2 cut(s) 524, 1212
AclWI GGATC 5 cut(s) 207, 220, 709, 1256, 1406
AcuI CTGAAG 1 cut(s) 404
AcyI GRCGYC 1 cut(s) 834
AfaI GTAC 2 cut(s) 1148, 1343
AfiI CCNNNNNNNGG 5 cut(s) 122, 981, 993, 1036, 1382
AflIII ACRYGT 1 cut(s) 951
AgsI TTSAA 6 cut(s) 12, 206, 623, 647, 1210, 1439
AjnI CCWGG 1 cut(s) 1375
AluBI AGCT 8 cut(s) 301, 389, 400, 472, 535, 909, 980, 1505
AluI AGCT 8 cut(s) 301, 389, 400, 472, 535, 909, 980, 1505
Alw26I GTCTC 3 cut(s) 373, 632, 1127
AlwI GGATC 5 cut(s) 207, 220, 709, 1256, 1406
AlwNI CAGNNNCTG 2 cut(s) 806, 925
AoxI GGCC 6 cut(s) 709, 1248, 1410, 1486, 1529, 1548
ApeKI GCWGC 4 cut(s) 301, 847, 1119, 1154
ArsI GACNNNNNNTTYG 2 cut(s) 261, 293
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 3 cut(s) 188, 984, 1248
AsuHPI GGTGA 7 cut(s) 256, 506, 571, 739, 1249, 1466, 1499
AsuNHI GCTAGC 1 cut(s) 1516
AvaII GGWCC 2 cut(s) 188, 984
AxyI CCTNAGG 1 cut(s) 1414
BamHI GGATCC 1 cut(s) 212
BanII GRGCYC 2 cut(s) 352, 1000
BauI CACGAG 1 cut(s) 1218
BbvCI CCTCAGC 1 cut(s) 976
BbvI GCAGC 4 cut(s) 288, 834, 1106, 1166
BccI CCATC 2 cut(s) 125, 1456
BciT130I CCWGG 1 cut(s) 1377
BciVI GTATCC 2 cut(s) 244, 742
BcoDI GTCTC 3 cut(s) 373, 632, 1127
BfaI CTAG 1 cut(s) 1517
BfmI CTRYAG 1 cut(s) 246
BfuI GTATCC 2 cut(s) 244, 742
BisI GCNGC 5 cut(s) 302, 848, 1120, 1155, 1158
BlsI GCNGC 5 cut(s) 303, 849, 1121, 1156, 1159
Bme1390I CCNGG 1 cut(s) 1377
Bme18I GGWCC 2 cut(s) 188, 984
BmgT120I GGNCC 3 cut(s) 188, 984, 1248
BmiI GGNNCC 4 cut(s) 214, 351, 985, 1307
BmrFI CCNGG 1 cut(s) 1377
BmrI ACTGGG 1 cut(s) 509
BmsI GCATC 2 cut(s) 888, 1131
BmtI GCTAGC 1 cut(s) 1520
BmuI ACTGGG 1 cut(s) 509
Bpu10I CCTNAGC 1 cut(s) 976
BpuEI CTTGAG 4 cut(s) 41, 397, 416, 734
BsaAI YACGTR 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 1127
BsaJI CCNNGG 4 cut(s) 825, 987, 1197, 1376
BsaWI WCCGGW 1 cut(s) 1395
BsaXI ACNNNNNCTCC 4 cut(s) 1357, 1387, 1438, 1468
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 981, 993, 1036, 1382
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 4 cut(s) 371, 504, 683, 1489
Bse21I CCTNAGG 1 cut(s) 1414
BseBI CCWGG 1 cut(s) 1377
BseDI CCNNGG 4 cut(s) 825, 987, 1197, 1376
BseGI GGATG 2 cut(s) 329, 407
BseLI CCNNNNNNNGG 5 cut(s) 122, 981, 993, 1036, 1382
BseMII CTCAG 3 cut(s) 813, 990, 1153
BseNI ACTGG 4 cut(s) 371, 504, 683, 1489
BseRI GAGGAG 2 cut(s) 44, 1472
BseXI GCAGC 4 cut(s) 288, 834, 1106, 1166
BseYI CCCAGC 3 cut(s) 566, 980, 1150
BsgI GTGCAG 3 cut(s) 462, 809, 1177
BshFI GGCC 6 cut(s) 711, 1250, 1412, 1488, 1531, 1550
BsiSI CCGG 2 cut(s) 309, 1396
BslFI GGGAC 3 cut(s) 997, 1369, 1495
BslI CCNNNNNNNGG 5 cut(s) 122, 981, 993, 1036, 1382
BsmAI GTCTC 3 cut(s) 373, 632, 1127
BsmBI CGTCTC 1 cut(s) 373
BsmFI GGGAC 3 cut(s) 997, 1369, 1495
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 6 cut(s) 711, 1250, 1412, 1488, 1531, 1550
Bso31I GGTCTC 1 cut(s) 1127
Bsp1286I GDGCHC 2 cut(s) 352, 1000
Bsp143I GATC 5 cut(s) 212, 701, 768, 1261, 1398
Bsp19I CCATGG 2 cut(s) 825, 987
BspACI CCGC 3 cut(s) 217, 1117, 1158
BspANI GGCC 6 cut(s) 711, 1250, 1412, 1488, 1531, 1550
BspCNI CTCAG 3 cut(s) 812, 989, 1152
BspHI TCATGA 1 cut(s) 1258
BspLI GGNNCC 4 cut(s) 214, 351, 985, 1307
BspMAI CTGCAG 1 cut(s) 250
BspOI GCTAGC 1 cut(s) 1520
BspPI GGATC 5 cut(s) 207, 220, 709, 1256, 1406
BspQI GCTCTTC 3 cut(s) 38, 809, 1272
BspTNI GGTCTC 1 cut(s) 1127
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 4 cut(s) 371, 504, 683, 1489
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 4 cut(s) 825, 987, 1197, 1376
BssMI GATC 5 cut(s) 212, 701, 768, 1261, 1398
BssNI GRCGYC 1 cut(s) 834
BssSI CACGAG 1 cut(s) 1218
BssT1I CCWWGG 2 cut(s) 825, 987
Bst2BI CACGAG 1 cut(s) 1218
Bst2UI CCWGG 1 cut(s) 1377
Bst4CI ACNGT 3 cut(s) 223, 293, 1198
Bst6I CTCTTC 5 cut(s) 38, 566, 737, 809, 1272
BstACI GRCGYC 1 cut(s) 834
BstBAI YACGTR 1 cut(s) 440
BstDEI CTNAG 5 cut(s) 799, 976, 1139, 1266, 1414
BstDSI CCRYGG 3 cut(s) 825, 987, 1197
BstENI CCTNNNNNAGG 2 cut(s) 1034, 1380
BstF5I GGATG 2 cut(s) 329, 407
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 5 cut(s) 215, 704, 771, 1264, 1401
BstMAI GTCTC 3 cut(s) 373, 632, 1127
BstMBI GATC 5 cut(s) 212, 701, 768, 1261, 1398
BstMWI GCNNNNNNNGC 2 cut(s) 1075, 1533
BstNI CCWGG 1 cut(s) 1377
BstNSI RCATGY 1 cut(s) 955
BstSCI CCNGG 1 cut(s) 1375
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 4 cut(s) 288, 834, 1106, 1166
BstX2I RGATCY 1 cut(s) 212
BstYI RGATCY 1 cut(s) 212
Bsu36I CCTNAGG 1 cut(s) 1414
BsuI GTATCC 2 cut(s) 244, 742
BsuRI GGCC 6 cut(s) 711, 1250, 1412, 1488, 1531, 1550
BtgI CCRYGG 3 cut(s) 825, 987, 1197
BtsCI GGATG 2 cut(s) 329, 407
BtsIMutI CAGTG 2 cut(s) 364, 1436
CaiI CAGNNNCTG 2 cut(s) 806, 925
CciI TCATGA 1 cut(s) 1258
CfoI GCGC 1 cut(s) 372
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 3 cut(s) 188, 984, 1248
CseI GACGC 1 cut(s) 390
Csp6I GTAC 2 cut(s) 1147, 1342
CviQI GTAC 2 cut(s) 1147, 1342
DdeI CTNAG 5 cut(s) 799, 976, 1139, 1266, 1414
DpnI GATC 5 cut(s) 214, 703, 770, 1263, 1400
DpnII GATC 5 cut(s) 212, 701, 768, 1261, 1398
Eam1104I CTCTTC 5 cut(s) 38, 566, 737, 809, 1272
EarI CTCTTC 5 cut(s) 38, 566, 737, 809, 1272
Eco130I CCWWGG 2 cut(s) 825, 987
Eco24I GRGCYC 2 cut(s) 352, 1000
Eco31I GGTCTC 1 cut(s) 1127
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 2 cut(s) 188, 984
Eco57I CTGAAG 1 cut(s) 404
Eco81I CCTNAGG 1 cut(s) 1414
EcoNI CCTNNNNNAGG 2 cut(s) 1034, 1380
EcoRII CCWGG 1 cut(s) 1375
EcoRV GATATC 1 cut(s) 592
EcoT14I CCWWGG 2 cut(s) 825, 987
EcoT38I GRGCYC 2 cut(s) 352, 1000
ErhI CCWWGG 2 cut(s) 825, 987
Esp3I CGTCTC 1 cut(s) 373
FaqI GGGAC 3 cut(s) 997, 1369, 1495
FauI CCCGC 1 cut(s) 224
Fnu4HI GCNGC 5 cut(s) 302, 848, 1120, 1155, 1158
FokI GGATG 2 cut(s) 336, 394
FriOI GRGCYC 2 cut(s) 352, 1000
Fsp4HI GCNGC 5 cut(s) 302, 848, 1120, 1155, 1158
FspBI CTAG 1 cut(s) 1517
GlaI GCGC 1 cut(s) 371
GluI GCNGC 5 cut(s) 302, 848, 1120, 1155, 1158
GsaI CCCAGC 3 cut(s) 570, 984, 1154
HaeIII GGCC 6 cut(s) 711, 1250, 1412, 1488, 1531, 1550
HapII CCGG 2 cut(s) 309, 1396
HgaI GACGC 1 cut(s) 390
HhaI GCGC 1 cut(s) 372
Hin1I GRCGYC 1 cut(s) 834
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HincII GTYRAC 2 cut(s) 522, 957
HindII GTYRAC 2 cut(s) 522, 957
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 5 cut(s) 87, 599, 922, 1088, 1178
HpaI GTTAAC 1 cut(s) 957
HpaII CCGG 2 cut(s) 309, 1396
HphI GGTGA 7 cut(s) 256, 506, 571, 739, 1249, 1466, 1499
Hpy166II GTNNAC 6 cut(s) 497, 522, 832, 957, 1240, 1468
Hpy188I TCNGA 5 cut(s) 423, 808, 1093, 1142, 1186
Hpy188III TCNNGA 2 cut(s) 58, 1259
Hpy8I GTNNAC 6 cut(s) 497, 522, 832, 957, 1240, 1468
HpyAV CCTTC 7 cut(s) 111, 212, 252, 1356, 1376, 1472, 1561
HpyCH4III ACNGT 3 cut(s) 223, 293, 1198
HpyCH4IV ACGT 5 cut(s) 439, 524, 752, 834, 1212
HpyF10VI GCNNNNNNNGC 2 cut(s) 1075, 1533
HpyF3I CTNAG 5 cut(s) 799, 976, 1139, 1266, 1414
HpySE526I ACGT 5 cut(s) 439, 524, 752, 834, 1212
Hsp92I GRCGYC 1 cut(s) 834
HspAI GCGC 1 cut(s) 370
KroI GCCGGC 1 cut(s) 308
KroNI GCCGGC 1 cut(s) 310
KspAI GTTAAC 1 cut(s) 957
Kzo9I GATC 5 cut(s) 212, 701, 768, 1261, 1398
LguI GCTCTTC 3 cut(s) 38, 809, 1272
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 4 cut(s) 288, 834, 1106, 1166
LweI GCATC 2 cut(s) 888, 1131
MaeI CTAG 1 cut(s) 1517
MaeII ACGT 5 cut(s) 439, 524, 752, 834, 1212
MaeIII GTNAC 3 cut(s) 277, 326, 456
MalI GATC 5 cut(s) 214, 703, 770, 1263, 1400
MboI GATC 5 cut(s) 212, 701, 768, 1261, 1398
MfeI CAATTG 1 cut(s) 647
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 2 cut(s) 352, 1000
MluCI AATT 9 cut(s) 206, 462, 627, 647, 731, 1050, 1062, 1080, 1289
MlyI GAGTC 3 cut(s) 81, 608, 1187
MroNI GCCGGC 1 cut(s) 308
MseI TTAA 4 cut(s) 141, 543, 956, 1293
MspA1I CMGCKG 2 cut(s) 980, 1119
MspI CCGG 2 cut(s) 309, 1396
MspR9I CCNGG 1 cut(s) 1377
MunI CAATTG 1 cut(s) 647
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 1 cut(s) 1377
MwoI GCNNNNNNNGC 2 cut(s) 1075, 1533
NaeI GCCGGC 1 cut(s) 310
NcoI CCATGG 2 cut(s) 825, 987
NdeII GATC 5 cut(s) 212, 701, 768, 1261, 1398
NgoMIV GCCGGC 1 cut(s) 308
NheI GCTAGC 1 cut(s) 1516
NlaIV GGNNCC 4 cut(s) 214, 351, 985, 1307
NmuCI GTSAC 2 cut(s) 277, 456
NspI RCATGY 1 cut(s) 955
PagI TCATGA 1 cut(s) 1258
PciI ACATGT 1 cut(s) 951
PciSI GCTCTTC 3 cut(s) 38, 809, 1272
PctI GAATGC 1 cut(s) 263
PdiI GCCGGC 1 cut(s) 310
PfeI GAWTC 2 cut(s) 922, 1088
PflMI CCANNNNNTGG 1 cut(s) 993
PkrI GCNGC 5 cut(s) 303, 849, 1121, 1156, 1159
PleI GAGTC 3 cut(s) 81, 607, 1186
PpsI GAGTC 3 cut(s) 81, 607, 1186
Ppu21I YACGTR 1 cut(s) 440
PscI ACATGT 1 cut(s) 951
Psp1406I AACGTT 2 cut(s) 524, 1212
Psp6I CCWGG 1 cut(s) 1375
PspFI CCCAGC 3 cut(s) 566, 980, 1150
PspGI CCWGG 1 cut(s) 1375
PspN4I GGNNCC 4 cut(s) 214, 351, 985, 1307
PspPI GGNCC 3 cut(s) 188, 984, 1248
PstI CTGCAG 1 cut(s) 250
PstNI CAGNNNCTG 2 cut(s) 806, 925
PsuI RGATCY 1 cut(s) 212
PvuII CAGCTG 1 cut(s) 980
RsaI GTAC 2 cut(s) 1148, 1343
RsaNI GTAC 2 cut(s) 1147, 1342
SapI GCTCTTC 3 cut(s) 38, 809, 1272
SaqAI TTAA 4 cut(s) 141, 543, 956, 1293
SatI GCNGC 5 cut(s) 302, 848, 1120, 1155, 1158
Sau3AI GATC 5 cut(s) 212, 701, 768, 1261, 1398
Sau96I GGNCC 3 cut(s) 188, 984, 1248
SchI GAGTC 3 cut(s) 81, 608, 1187
ScrFI CCNGG 1 cut(s) 1377
SduI GDGCHC 2 cut(s) 352, 1000
SfaNI GCATC 2 cut(s) 888, 1131
SfcI CTRYAG 1 cut(s) 246
SinI GGWCC 2 cut(s) 188, 984
SmlI CTYRAG 4 cut(s) 56, 376, 395, 713
SmoI CTYRAG 4 cut(s) 56, 376, 395, 713
Sse9I AATT 9 cut(s) 206, 462, 627, 647, 731, 1050, 1062, 1080, 1289
SsiI CCGC 3 cut(s) 217, 1117, 1158
SspI AATATT 3 cut(s) 667, 764, 869
SspMI CTAG 1 cut(s) 1517
StyD4I CCNGG 1 cut(s) 1375
StyI CCWWGG 2 cut(s) 825, 987
TaaI ACNGT 3 cut(s) 223, 293, 1198
TaiI ACGT 5 cut(s) 442, 527, 755, 837, 1215
TaqI TCGA 3 cut(s) 478, 531, 771
TasI AATT 9 cut(s) 206, 462, 627, 647, 731, 1050, 1062, 1080, 1289
TatI WGTACW 1 cut(s) 1341
TauI GCSGC 1 cut(s) 1160
TfiI GAWTC 2 cut(s) 922, 1088
Tru1I TTAA 4 cut(s) 141, 543, 956, 1293
Tru9I TTAA 4 cut(s) 141, 543, 956, 1293
TscAI CASTG 2 cut(s) 371, 1436
TseFI GTSAC 2 cut(s) 277, 456
TseI GCWGC 4 cut(s) 301, 847, 1119, 1154
Tsp45I GTSAC 2 cut(s) 277, 456
TspDTI ATGAA 9 cut(s) 25, 419, 462, 530, 745, 894, 1327, 1487, 1491
TspRI CASTG 2 cut(s) 371, 1436
Van91I CCANNNNNTGG 1 cut(s) 993
VpaK11BI GGWCC 2 cut(s) 188, 984
XagI CCTNNNNNAGG 2 cut(s) 1034, 1380
XceI RCATGY 1 cut(s) 955
XspI CTAG 1 cut(s) 1517
ZraI GACGTC 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.