FvH4_2g33490

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002267
Physical Location & Seq
Forward (+)
25107087 .. 25109068
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33490.t1

Sequence Viewer

Length: 1518 bp
ATGGAAACTTCAATCTCCTACTACCTTTGGCTCTTCATTGTCTTTCTTTACCTCCTCAGGAAGCATTTTCAGAAAACTAACAAAAGACTCCCACCAAGCCCTTCTCTTTCCTTTCCCATCATAGGTCATCTCTACCTCTTCAAGAAACCTCTCCACAGAACGCTTGCAAAACTATCTGAAAAATATGGTCCAGTTTTATACCTTAAACTTGGATCCCGCCCGGTTCTTATTGTATCCTCACCATCTGCTGCTGAAGAATGCTTCACCAAAAACGACATCTCTTTCGCAAACCGCCCTAAACTGCTTGCTGGCAAACACCTTGGATGTAACTATACCACGGTTATGTGGGCACCCTATGGCTCTCACTGGCGCAACATGAGACGCATAGCTTCCATTGAGCTATTGTCCTCCAGCCGCCTTCAGATGTGTCATGGCATACGTGCAGATGAAGTTAGGTCACTAATTTGCAAGCTTTTTCGAGGGTCTAATGGTGGTGAGTTTCACATTGTGGACATGAAGACAACGTTTTTTGAGCTGACTCTTAATGTCTTGATGAGAGTGATTGCTGGGAAGAGATACTACGGGGAAGACATCGCAGATTCCAGTGAAGCTAAGAGATTTGAACAACTTGTGAAAGAGAGCGTTCACTTGACTGGGGCTAACTATATTGGAGATTATTTGCCAGTTTTGAAGTACGTTGGATCTGGTAGCCTTGAAAAAAGGTTGGTGAAATTGCAAAAGAAGAGGGATGAGTTCATGCAATCTCTGATCGAAGAACGCAGAAAAGTGCAGGGTGGCTGTGTTTCTGAACAGAAGAGCAAGACCATGGTGGATGTCTTGCTGTCACTGCAGGCAACTGAACCTGAATATTATACTGACGAAATCATAAGAGGCATGATGCAAATCATGTTAACAGCAGGAACTGACACCTCAGCTGGTAACATGGAGTGGACTCTGTCACTGTTACTCAACAACCCAGAAGCCCTAGCAAAAGCACAGACAGAAATTGATACCAAAATTGGACAAAGCAGGCTAATTGTGGAATCAGACCTTGCTAATCTCCCCTATCTCCATTGCATCATACGTGAGACCCTCAGAATGTACCCACCAGCCACAATTCTGCATGAGTCATCGGAAGACTCCACCGTCGGAGGGTTCAACGTTCCTCGTGGTACAATGCTTATAATGAACGCATGGGCGATACATCATGATCCTAAGTTGTGGGAACAGCCTGAACAATTTAAGCCGGAGAGGTTCCAAATTGAACAAGAAGAAAGAGATGGTTTCAAGTACATGCCATTTGGGACAGGAAGGAGAGGCTGTCCTGGGGAAGGGTTAGCCAATCGGATTGTAGGGCTGGCCATAGGGTCAGTCATTCAGTGTTTTGAATGGGAGAGAAGTGGTGAGGAGATGGTGGACATGACTGAAGGGACTGGCCTCACAATCTTTAAAGCTCATCCTTTGCTAGCAAAATGCAGGCCGCGCCAAACAATGCTGCCCTTCCTTTCTCAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

506

Amino Acids

57.48

Weight (kDa)

8.85

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 32 - 480 2.5e-95 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1184
AasI GACNNNNNNGTC 1 cut(s) 1145
AccB1I GGYRCC 1 cut(s) 349
AccII CGCG 1 cut(s) 1483
AciI CCGC 4 cut(s) 217, 292, 415, 1481
AclI AACGTT 2 cut(s) 524, 1161
AclWI GGATC 4 cut(s) 207, 220, 709, 1205
AcoI YGGCCR 1 cut(s) 1359
AcuI CTGAAG 3 cut(s) 273, 404, 1446
AdeI CACNNNGTG 1 cut(s) 508
AfaI GTAC 4 cut(s) 695, 1103, 1174, 1292
AfiI CCNNNNNNNGG 3 cut(s) 122, 1152, 1331
AgsI TTSAA 9 cut(s) 12, 142, 623, 691, 716, 1159, 1265, 1288, 1388
AjnI CCWGG 1 cut(s) 1324
AluBI AGCT 7 cut(s) 389, 400, 472, 535, 611, 935, 1454
AluI AGCT 7 cut(s) 389, 400, 472, 535, 611, 935, 1454
Alw26I GTCTC 2 cut(s) 373, 1082
AlwI GGATC 4 cut(s) 207, 220, 709, 1205
AlwNI CAGNNNCTG 1 cut(s) 923
AoxI GGCC 3 cut(s) 1359, 1435, 1478
ApeKI GCWGC 2 cut(s) 248, 1495
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
AspLEI GCGC 2 cut(s) 372, 1485
AspS9I GGNCC 1 cut(s) 188
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 5 cut(s) 231, 256, 506, 739, 1415
AsuNHI GCTAGC 1 cut(s) 1465
AvaII GGWCC 1 cut(s) 188
AxyI CCTNAGG 1 cut(s) 56
BaeGI GKGCMC 1 cut(s) 352
BalI TGGCCA 1 cut(s) 1361
BamHI GGATCC 1 cut(s) 212
BanI GGYRCC 1 cut(s) 349
BarI GAAGNNNNNNTAC 2 cut(s) 563, 595
BauI CACGAG 1 cut(s) 1167
BbsI GAAGAC 3 cut(s) 524, 594, 1143
BbvCI CCTCAGC 1 cut(s) 931
BbvI GCAGC 2 cut(s) 235, 1482
BccI CCATC 4 cut(s) 125, 250, 1274, 1405
BciT130I CCWGG 1 cut(s) 1326
BciVI GTATCC 1 cut(s) 244
BcnI CCSGG 1 cut(s) 221
BcoDI GTCTC 2 cut(s) 373, 1082
BfaI CTAG 3 cut(s) 986, 1466, 1516
BfmI CTRYAG 1 cut(s) 848
BfuI GTATCC 1 cut(s) 244
BisI GCNGC 4 cut(s) 249, 415, 1481, 1496
BlsI GCNGC 4 cut(s) 250, 416, 1482, 1497
Bme1390I CCNGG 2 cut(s) 221, 1326
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 3 cut(s) 214, 351, 1256
BmrFI CCNGG 2 cut(s) 221, 1326
BmrI ACTGGG 1 cut(s) 663
BmsI GCATC 2 cut(s) 888, 1086
BmtI GCTAGC 1 cut(s) 1469
BmuI ACTGGG 1 cut(s) 663
BpiI GAAGAC 3 cut(s) 524, 594, 1143
BpmI CTGGAG 1 cut(s) 394
Bpu10I CCTNAGC 1 cut(s) 931
BpuMI CCSGG 1 cut(s) 221
BsaAI YACGTR 2 cut(s) 440, 1085
BsaBI GATNNNNATC 1 cut(s) 902
BsaI GGTCTC 1 cut(s) 1082
BsaJI CCNNGG 4 cut(s) 319, 336, 825, 1325
BsaXI ACNNNNNCTCC 2 cut(s) 1306, 1336
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 1152, 1331
Bse1I ACTGG 6 cut(s) 191, 371, 603, 658, 683, 1438
Bse21I CCTNAGG 1 cut(s) 56
Bse3DI GCAATG 1 cut(s) 1072
Bse8I GATNNNNATC 1 cut(s) 902
BseBI CCWGG 1 cut(s) 1326
BseDI CCNNGG 4 cut(s) 319, 336, 825, 1325
BseGI GGATG 4 cut(s) 329, 754, 838, 1456
BseJI GATNNNNATC 1 cut(s) 902
BseLI CCNNNNNNNGG 3 cut(s) 122, 1152, 1331
BseMI GCAATG 1 cut(s) 1072
BseMII CTCAG 3 cut(s) 70, 945, 1108
BseNI ACTGG 6 cut(s) 191, 371, 603, 658, 683, 1438
BseRI GAGGAG 2 cut(s) 44, 1421
BseSI GKGCMC 1 cut(s) 352
BseXI GCAGC 2 cut(s) 235, 1482
BseYI CCCAGC 1 cut(s) 566
BsgI GTGCAG 2 cut(s) 462, 809
Bsh1236I CGCG 1 cut(s) 1483
BshFI GGCC 3 cut(s) 1361, 1437, 1480
BshNI GGYRCC 1 cut(s) 349
BsiSI CCGG 2 cut(s) 221, 1247
BslFI GGGAC 2 cut(s) 1318, 1444
BslI CCNNNNNNNGG 3 cut(s) 122, 1152, 1331
BsmAI GTCTC 2 cut(s) 373, 1082
BsmBI CGTCTC 1 cut(s) 373
BsmFI GGGAC 2 cut(s) 1318, 1444
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 3 cut(s) 1361, 1437, 1480
Bso31I GGTCTC 1 cut(s) 1082
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 4 cut(s) 212, 701, 768, 1210
Bsp19I CCATGG 1 cut(s) 825
BspACI CCGC 4 cut(s) 217, 292, 415, 1481
BspANI GGCC 3 cut(s) 1361, 1437, 1480
BspCNI CTCAG 3 cut(s) 69, 944, 1107
BspFNI CGCG 1 cut(s) 1483
BspHI TCATGA 1 cut(s) 1207
BspLI GGNNCC 3 cut(s) 214, 351, 1256
BspMAI CTGCAG 1 cut(s) 852
BspOI GCTAGC 1 cut(s) 1469
BspPI GGATC 4 cut(s) 207, 220, 709, 1205
BspQI GCTCTTC 2 cut(s) 38, 809
BspT107I GGYRCC 1 cut(s) 349
BspTNI GGTCTC 1 cut(s) 1082
BsrDI GCAATG 1 cut(s) 1072
BsrI ACTGG 6 cut(s) 191, 371, 603, 658, 683, 1438
BssECI CCNNGG 4 cut(s) 319, 336, 825, 1325
BssMI GATC 4 cut(s) 212, 701, 768, 1210
BssSI CACGAG 1 cut(s) 1167
BssT1I CCWWGG 2 cut(s) 319, 825
Bst2BI CACGAG 1 cut(s) 1167
Bst2UI CCWGG 1 cut(s) 1326
Bst4CI ACNGT 3 cut(s) 340, 963, 1147
Bst6I CTCTTC 5 cut(s) 38, 143, 566, 737, 809
BstBAI YACGTR 2 cut(s) 440, 1085
BstC8I GCNNGC 9 cut(s) 165, 306, 310, 470, 852, 1031, 1359, 1467, 1478
BstDEI CTNAG 5 cut(s) 56, 612, 931, 1094, 1215
BstDSI CCRYGG 2 cut(s) 336, 825
BstENI CCTNNNNNAGG 1 cut(s) 1329
BstF5I GGATG 4 cut(s) 329, 754, 838, 1456
BstFNI CGCG 1 cut(s) 1483
BstHHI GCGC 2 cut(s) 372, 1485
BstKTI GATC 4 cut(s) 215, 704, 771, 1213
BstMAI GTCTC 2 cut(s) 373, 1082
BstMBI GATC 4 cut(s) 212, 701, 768, 1210
BstMWI GCNNNNNNNGC 2 cut(s) 847, 1482
BstNI CCWGG 1 cut(s) 1326
BstNSI RCATGY 1 cut(s) 1297
BstSCI CCNGG 2 cut(s) 219, 1324
BstSFI CTRYAG 1 cut(s) 848
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 1 cut(s) 1483
BstV1I GCAGC 2 cut(s) 235, 1482
BstV2I GAAGAC 3 cut(s) 524, 594, 1143
BstX2I RGATCY 2 cut(s) 212, 701
BstYI RGATCY 2 cut(s) 212, 701
Bsu36I CCTNAGG 1 cut(s) 56
BsuI GTATCC 1 cut(s) 244
BsuRI GGCC 3 cut(s) 1361, 1437, 1480
BtgI CCRYGG 2 cut(s) 336, 825
BtgZI GCGATG 1 cut(s) 577
BtsCI GGATG 4 cut(s) 329, 754, 838, 1456
BtsI GCAGTG 1 cut(s) 845
BtsIMutI CAGTG 5 cut(s) 364, 610, 845, 959, 1385
Cac8I GCNNGC 9 cut(s) 165, 306, 310, 470, 852, 1031, 1359, 1467, 1478
CaiI CAGNNNCTG 1 cut(s) 923
CciI TCATGA 1 cut(s) 1207
CfoI GCGC 2 cut(s) 372, 1485
Cfr13I GGNCC 1 cut(s) 188
CseI GACGC 1 cut(s) 390
Csp6I GTAC 4 cut(s) 694, 1102, 1173, 1291
CviQI GTAC 4 cut(s) 694, 1102, 1173, 1291
DdeI CTNAG 5 cut(s) 56, 612, 931, 1094, 1215
DpnI GATC 4 cut(s) 214, 703, 770, 1212
DpnII GATC 4 cut(s) 212, 701, 768, 1210
DraI TTTAAA 1 cut(s) 1450
DraIII CACNNNGTG 1 cut(s) 508
DrdI GACNNNNNNGTC 1 cut(s) 1145
DseDI GACNNNNNNGTC 1 cut(s) 1145
EaeI YGGCCR 1 cut(s) 1359
Eam1104I CTCTTC 5 cut(s) 38, 143, 566, 737, 809
EarI CTCTTC 5 cut(s) 38, 143, 566, 737, 809
Eco130I CCWWGG 2 cut(s) 319, 825
Eco31I GGTCTC 1 cut(s) 1082
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 3 cut(s) 273, 404, 1446
Eco81I CCTNAGG 1 cut(s) 56
EcoNI CCTNNNNNAGG 1 cut(s) 1329
EcoRII CCWGG 1 cut(s) 1324
EcoT14I CCWWGG 2 cut(s) 319, 825
ErhI CCWWGG 2 cut(s) 319, 825
Esp3I CGTCTC 1 cut(s) 373
FaqI GGGAC 2 cut(s) 1318, 1444
FauI CCCGC 1 cut(s) 224
Fnu4HI GCNGC 4 cut(s) 249, 415, 1481, 1496
FokI GGATG 4 cut(s) 336, 761, 845, 1443
Fsp4HI GCNGC 4 cut(s) 249, 415, 1481, 1496
FspBI CTAG 3 cut(s) 986, 1466, 1516
GlaI GCGC 2 cut(s) 371, 1484
GluI GCNGC 4 cut(s) 249, 415, 1481, 1496
GsaI CCCAGC 1 cut(s) 570
GsuI CTGGAG 1 cut(s) 394
HaeIII GGCC 3 cut(s) 1361, 1437, 1480
HapII CCGG 2 cut(s) 221, 1247
HgaI GACGC 1 cut(s) 390
HhaI GCGC 2 cut(s) 372, 1485
Hin6I GCGC 2 cut(s) 370, 1483
HinP1I GCGC 2 cut(s) 370, 1483
HincII GTYRAC 1 cut(s) 912
HindII GTYRAC 1 cut(s) 912
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 7 cut(s) 87, 538, 599, 952, 1043, 1127, 1139
HpaI GTTAAC 1 cut(s) 912
HpaII CCGG 2 cut(s) 221, 1247
HphI GGTGA 5 cut(s) 231, 256, 506, 739, 1415
Hpy166II GTNNAC 5 cut(s) 511, 646, 912, 951, 1417
Hpy188III TCNNGA 4 cut(s) 58, 142, 550, 1208
Hpy8I GTNNAC 5 cut(s) 511, 646, 912, 951, 1417
Hpy99I CGWCG 1 cut(s) 1151
HpyAV CCTTC 6 cut(s) 111, 428, 1305, 1325, 1421, 1510
HpyCH4III ACNGT 3 cut(s) 340, 963, 1147
HpyCH4IV ACGT 5 cut(s) 439, 524, 696, 1084, 1161
HpyF10VI GCNNNNNNNGC 2 cut(s) 847, 1482
HpyF3I CTNAG 5 cut(s) 56, 612, 931, 1094, 1215
HpySE526I ACGT 5 cut(s) 439, 524, 696, 1084, 1161
HspAI GCGC 2 cut(s) 370, 1483
KspAI GTTAAC 1 cut(s) 912
Kzo9I GATC 4 cut(s) 212, 701, 768, 1210
LguI GCTCTTC 2 cut(s) 38, 809
Lsp1109I GCAGC 2 cut(s) 235, 1482
LweI GCATC 2 cut(s) 888, 1086
MaeI CTAG 3 cut(s) 986, 1466, 1516
MaeII ACGT 5 cut(s) 439, 524, 696, 1084, 1161
MaeIII GTNAC 6 cut(s) 326, 456, 843, 938, 957, 963
MalI GATC 4 cut(s) 214, 703, 770, 1212
MboI GATC 4 cut(s) 212, 701, 768, 1210
MflI RGATCY 2 cut(s) 212, 701
MhlI GDGCHC 1 cut(s) 352
MlsI TGGCCA 1 cut(s) 1361
MluCI AATT 8 cut(s) 462, 731, 1005, 1017, 1035, 1116, 1238, 1260
MluNI TGGCCA 1 cut(s) 1361
MlyI GAGTC 5 cut(s) 81, 532, 946, 1133, 1136
MmeI TCCRAC 2 cut(s) 679, 1129
Mox20I TGGCCA 1 cut(s) 1361
MscI TGGCCA 1 cut(s) 1361
MseI TTAA 5 cut(s) 204, 543, 911, 1242, 1449
MslI CAYNNNNRTG 1 cut(s) 341
Msp20I TGGCCA 1 cut(s) 1361
MspA1I CMGCKG 1 cut(s) 935
MspI CCGG 2 cut(s) 221, 1247
MspR9I CCNGG 2 cut(s) 221, 1326
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 1 cut(s) 1326
MvnI CGCG 1 cut(s) 1483
MwoI GCNNNNNNNGC 2 cut(s) 847, 1482
NciI CCSGG 1 cut(s) 221
NcoI CCATGG 1 cut(s) 825
NdeII GATC 4 cut(s) 212, 701, 768, 1210
NheI GCTAGC 1 cut(s) 1465
NlaIV GGNNCC 3 cut(s) 214, 351, 1256
NmuCI GTSAC 3 cut(s) 456, 843, 957
NspI RCATGY 1 cut(s) 1297
PagI TCATGA 1 cut(s) 1207
PciSI GCTCTTC 2 cut(s) 38, 809
PctI GAATGC 1 cut(s) 263
PfeI GAWTC 2 cut(s) 599, 1043
PflFI GACNNNGTC 1 cut(s) 955
PkrI GCNGC 4 cut(s) 250, 416, 1482, 1497
PleI GAGTC 5 cut(s) 81, 532, 946, 1133, 1135
PpsI GAGTC 5 cut(s) 81, 532, 946, 1133, 1135
Ppu21I YACGTR 2 cut(s) 440, 1085
PsiI TTATAA 1 cut(s) 1184
Psp1406I AACGTT 2 cut(s) 524, 1161
Psp6I CCWGG 1 cut(s) 1324
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 1 cut(s) 1324
PspN4I GGNNCC 3 cut(s) 214, 351, 1256
PspPI GGNCC 1 cut(s) 188
PstI CTGCAG 1 cut(s) 852
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 2 cut(s) 212, 701
PsyI GACNNNGTC 1 cut(s) 955
PvuII CAGCTG 1 cut(s) 935
RsaI GTAC 4 cut(s) 695, 1103, 1174, 1292
RsaNI GTAC 4 cut(s) 694, 1102, 1173, 1291
RseI CAYNNNNRTG 1 cut(s) 341
SapI GCTCTTC 2 cut(s) 38, 809
SaqAI TTAA 5 cut(s) 204, 543, 911, 1242, 1449
SatI GCNGC 4 cut(s) 249, 415, 1481, 1496
Sau3AI GATC 4 cut(s) 212, 701, 768, 1210
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 5 cut(s) 81, 532, 946, 1133, 1136
ScrFI CCNGG 2 cut(s) 221, 1326
SduI GDGCHC 1 cut(s) 352
SfaNI GCATC 2 cut(s) 888, 1086
SfcI CTRYAG 1 cut(s) 848
SinI GGWCC 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 341
Sse9I AATT 8 cut(s) 462, 731, 1005, 1017, 1035, 1116, 1238, 1260
SsiI CCGC 4 cut(s) 217, 292, 415, 1481
SspI AATATT 1 cut(s) 869
SspMI CTAG 3 cut(s) 986, 1466, 1516
StyD4I CCNGG 2 cut(s) 219, 1324
StyI CCWWGG 2 cut(s) 319, 825
TaaI ACNGT 3 cut(s) 340, 963, 1147
TaiI ACGT 5 cut(s) 442, 527, 699, 1087, 1164
TaqI TCGA 2 cut(s) 478, 771
TasI AATT 8 cut(s) 462, 731, 1005, 1017, 1035, 1116, 1238, 1260
TatI WGTACW 1 cut(s) 1290
TauI GCSGC 2 cut(s) 417, 1483
TfiI GAWTC 2 cut(s) 599, 1043
Tru1I TTAA 5 cut(s) 204, 543, 911, 1242, 1449
Tru9I TTAA 5 cut(s) 204, 543, 911, 1242, 1449
TscAI CASTG 5 cut(s) 371, 610, 852, 966, 1385
TseFI GTSAC 3 cut(s) 456, 843, 957
TseI GCWGC 2 cut(s) 248, 1495
Tsp45I GTSAC 3 cut(s) 456, 843, 957
TspDTI ATGAA 5 cut(s) 25, 462, 530, 745, 1202
TspRI CASTG 5 cut(s) 371, 610, 852, 966, 1385
Tth111I GACNNNGTC 1 cut(s) 955
VpaK11BI GGWCC 1 cut(s) 188
XagI CCTNNNNNAGG 1 cut(s) 1329
XceI RCATGY 1 cut(s) 1297
XspI CTAG 3 cut(s) 986, 1466, 1516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.