pycom15g06960

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002267
Physical Location & Seq
Forward (+)
4245905 .. 4247390
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g06960.1

Sequence Viewer

Length: 981 bp
ATGGACCAATCACATACCTCTGATTCGGCTCCCGCCCTGTCCTCCTCGTATCCTCACCTTCGGCGGCTGAGGAATGCTTCACCAAAAACGACATCACTTTTGCTAACCGCCCGAAGCTGCTTGCCGGAAAACACCTCGGATACAACTACACCACCCTCACCTGGGCCTCTTACGACTCCCACTGTTCAGATGCTTTCTAGCATCCGTCTCGATGAAGTCAGGTCCTTGATTTCCCGGCTCTTTCGAGGCACGAAAGGCAGCAGCGAATTTCAGAGTTTGGACATGAAGTCGGCGTTTTTCGAGCTGACTCTTAATGTTCTGATGAGGATGGTTGCGGGAAAGCGGTGCCATGGGGAAAATGTGGCGGATTTAAAGGAGATTATTGCTGAGAACTTTCGGTTGAGTGGCGCTACCAATCTTGGAGATTTTCTGCCGGTTTTGAAGTATGTGGGGGTAAGTCGCCTTGAGAAGAAATTGATGACACTCCACAAGAAAATGGAAAAATTAATGCAAGATTTGATCCAAGAGCGCAGAGAAAATCAGAGTGGCTCTGTTTCTGAACAGAGGAGCAAGTCCATGGCGGACGCCTTGCTTGCTCTGCAAGAAACTGAACCGGAATATTATACTGATGAGATCGTAAGAGGCATGATGCAAGTCATGTTAGCAGCAGGGACAGACACCTCATCTGGAACCATGGAATGGGCTCTATCACTTTTGCTCAACAACCCAGAAGCCCTAGCAAAAACTCAGACTGAAATGGATATCCACATTGGGCAATGCAGGCTACTTGAGGAGTCCGACCTCGCCAAGCTTCCGTATCTCCACGGCATCATCAATGAAACCCTCCGGATGTACCCAGCAGGCCCTTTGCTTGCCCCACACGAGTCATCCGAGGACTGCACAGTGGGGGGTTTCCACGTTCCACGTGGCACGTTGTTGCTGATGAACTTATGGGCCATACAGAACAATCCAAAGCTGTGA

Protein Analysis

327

Amino Acids

36.18

Weight (kDa)

6.1

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 67 - 325 3e-51 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 345
AccB7I CCANNNNNTGG 1 cut(s) 699
AccIII TCCGGA 1 cut(s) 846
AciI CCGC 7 cut(s) 33, 64, 108, 335, 343, 365, 581
AclWI GGATC 1 cut(s) 514
AcsI RAATTY 1 cut(s) 266
AcvI CACGTG 1 cut(s) 926
AcyI GRCGYC 1 cut(s) 585
AfaI GTAC 1 cut(s) 854
AfiI CCNNNNNNNGG 3 cut(s) 161, 162, 699
AgsI TTSAA 1 cut(s) 442
AhdI GACNNNNNGTC 1 cut(s) 286
AjnI CCWGG 1 cut(s) 160
AluBI AGCT 4 cut(s) 117, 304, 811, 976
AluI AGCT 4 cut(s) 117, 304, 811, 976
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 1 cut(s) 514
Aor13HI TCCGGA 1 cut(s) 846
AoxI GGCC 3 cut(s) 164, 862, 954
ApeKI GCWGC 4 cut(s) 117, 258, 261, 665
ApoI RAATTY 1 cut(s) 266
ArsI GACNNNNNNTTYG 2 cut(s) 82, 114
AseI ATTAAT 1 cut(s) 506
AspLEI GCGC 2 cut(s) 410, 531
AspS9I GGNCC 5 cut(s) 4, 164, 222, 863, 954
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 3 cut(s) 47, 72, 150
AvaII GGWCC 2 cut(s) 4, 222
BanI GGYRCC 1 cut(s) 345
BanII GRGCYC 1 cut(s) 706
BauI CACGAG 1 cut(s) 881
BbrPI CACGTG 1 cut(s) 926
BbvCI CCTCAGC 1 cut(s) 68
BbvI GCAGC 4 cut(s) 104, 270, 273, 677
BccI CCATC 1 cut(s) 322
BceAI ACGGC 1 cut(s) 841
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 1 cut(s) 162
BciVI GTATCC 2 cut(s) 60, 133
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 2 cut(s) 198, 737
BfoI RGCGCY 1 cut(s) 411
BfuI GTATCC 2 cut(s) 60, 133
BisI GCNGC 5 cut(s) 65, 118, 259, 262, 666
BlsI GCNGC 5 cut(s) 66, 119, 260, 263, 667
Bme1390I CCNGG 2 cut(s) 162, 235
Bme18I GGWCC 2 cut(s) 4, 222
BmeRI GACNNNNNGTC 1 cut(s) 286
BmgT120I GGNCC 5 cut(s) 4, 164, 222, 863, 954
BmiI GGNNCC 3 cut(s) 30, 347, 691
BmrFI CCNGG 2 cut(s) 162, 235
BmsI GCATC 4 cut(s) 180, 210, 639, 837
Bpu10I CCTNAGC 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 485, 809
BpuMI CCSGG 1 cut(s) 235
BsaAI YACGTR 1 cut(s) 926
BsaHI GRCGYC 1 cut(s) 585
BsaJI CCNNGG 7 cut(s) 135, 161, 349, 576, 693, 823, 891
BsaWI WCCGGW 2 cut(s) 613, 846
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 162, 699
Bse118I RCCGGY 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 782
BseAI TCCGGA 1 cut(s) 846
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 7 cut(s) 135, 161, 349, 576, 693, 823, 891
BseGI GGATG 4 cut(s) 201, 333, 855, 887
BseLI CCNNNNNNNGG 3 cut(s) 161, 162, 699
BseMI GCAATG 1 cut(s) 782
BseMII CTCAG 3 cut(s) 59, 378, 761
BseRI GAGGAG 3 cut(s) 34, 580, 806
BseXI GCAGC 4 cut(s) 104, 270, 273, 677
BseYI CCCAGC 1 cut(s) 856
BsgI GTGCAG 1 cut(s) 883
BshFI GGCC 3 cut(s) 166, 864, 956
BshNI GGYRCC 1 cut(s) 345
BsiSI CCGG 5 cut(s) 125, 235, 434, 614, 847
BslFI GGGAC 1 cut(s) 685
BslI CCNNNNNNNGG 3 cut(s) 161, 162, 699
BsmAI GTCTC 1 cut(s) 212
BsmBI CGTCTC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 685
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 3 cut(s) 166, 864, 956
Bsp1286I GDGCHC 1 cut(s) 706
Bsp13I TCCGGA 1 cut(s) 846
Bsp143I GATC 2 cut(s) 519, 633
Bsp19I CCATGG 3 cut(s) 349, 576, 693
BspACI CCGC 7 cut(s) 33, 64, 108, 335, 343, 365, 581
BspANI GGCC 3 cut(s) 166, 864, 956
BspCNI CTCAG 3 cut(s) 60, 379, 760
BspEI TCCGGA 1 cut(s) 846
BspLI GGNNCC 3 cut(s) 30, 347, 691
BspPI GGATC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 782
BsrFI RCCGGY 1 cut(s) 433
BssAI RCCGGY 1 cut(s) 433
BssECI CCNNGG 7 cut(s) 135, 161, 349, 576, 693, 823, 891
BssMI GATC 2 cut(s) 519, 633
BssNI GRCGYC 1 cut(s) 585
BssSI CACGAG 1 cut(s) 881
BssT1I CCWWGG 3 cut(s) 349, 576, 693
Bst2BI CACGAG 1 cut(s) 881
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 184, 904
BstACI GRCGYC 1 cut(s) 585
BstBAI YACGTR 1 cut(s) 926
BstC8I GCNNGC 5 cut(s) 122, 594, 782, 862, 873
BstDEI CTNAG 3 cut(s) 68, 387, 747
BstDSI CCRYGG 4 cut(s) 349, 576, 693, 823
BstF5I GGATG 4 cut(s) 201, 333, 855, 887
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 2 cut(s) 410, 531
BstKTI GATC 2 cut(s) 522, 636
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 2 cut(s) 519, 633
BstMWI GCNNNNNNNGC 4 cut(s) 255, 593, 598, 781
BstNI CCWGG 1 cut(s) 162
BstSCI CCNGG 2 cut(s) 160, 233
BstV1I GCAGC 4 cut(s) 104, 270, 273, 677
BsuI GTATCC 2 cut(s) 60, 133
BsuRI GGCC 3 cut(s) 166, 864, 956
BtgI CCRYGG 4 cut(s) 349, 576, 693, 823
BtsCI GGATG 4 cut(s) 201, 333, 855, 887
BtsIMutI CAGTG 2 cut(s) 180, 909
Cac8I GCNNGC 5 cut(s) 122, 594, 782, 862, 873
CfoI GCGC 2 cut(s) 410, 531
Cfr10I RCCGGY 1 cut(s) 433
Cfr13I GGNCC 5 cut(s) 4, 164, 222, 863, 954
CseI GACGC 1 cut(s) 593
Csp6I GTAC 1 cut(s) 853
CviAII CATG 6 cut(s) 283, 350, 577, 646, 658, 694
CviQI GTAC 1 cut(s) 853
DdeI CTNAG 3 cut(s) 68, 387, 747
DpnI GATC 2 cut(s) 521, 635
DpnII GATC 2 cut(s) 519, 633
DraI TTTAAA 1 cut(s) 372
DriI GACNNNNNGTC 1 cut(s) 286
Eam1105I GACNNNNNGTC 1 cut(s) 286
EciI GGCGGA 2 cut(s) 380, 596
Eco130I CCWWGG 3 cut(s) 349, 576, 693
Eco24I GRGCYC 1 cut(s) 706
Eco32I GATATC 1 cut(s) 763
Eco47I GGWCC 2 cut(s) 4, 222
Eco72I CACGTG 1 cut(s) 926
EcoO109I RGGNCCY 2 cut(s) 222, 863
EcoRII CCWGG 1 cut(s) 160
EcoRV GATATC 1 cut(s) 763
EcoT14I CCWWGG 3 cut(s) 349, 576, 693
EcoT38I GRGCYC 1 cut(s) 706
ErhI CCWWGG 3 cut(s) 349, 576, 693
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 6 cut(s) 286, 353, 580, 649, 661, 697
FaqI GGGAC 1 cut(s) 685
FatI CATG 6 cut(s) 282, 349, 576, 645, 657, 693
FauI CCCGC 2 cut(s) 40, 328
Fnu4HI GCNGC 5 cut(s) 65, 118, 259, 262, 666
FokI GGATG 4 cut(s) 188, 340, 862, 874
FriOI GRGCYC 1 cut(s) 706
Fsp4HI GCNGC 5 cut(s) 65, 118, 259, 262, 666
FspBI CTAG 2 cut(s) 198, 737
GlaI GCGC 2 cut(s) 409, 530
GluI GCNGC 5 cut(s) 65, 118, 259, 262, 666
GsaI CCCAGC 1 cut(s) 860
HaeII RGCGCY 1 cut(s) 411
HaeIII GGCC 3 cut(s) 166, 864, 956
HapII CCGG 5 cut(s) 125, 235, 434, 614, 847
HgaI GACGC 1 cut(s) 593
HhaI GCGC 2 cut(s) 410, 531
Hin1I GRCGYC 1 cut(s) 585
Hin1II CATG 6 cut(s) 286, 353, 580, 649, 661, 697
Hin6I GCGC 2 cut(s) 408, 529
HinP1I GCGC 2 cut(s) 408, 529
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 5 cut(s) 23, 175, 307, 794, 884
HpaII CCGG 5 cut(s) 125, 235, 434, 614, 847
HphI GGTGA 3 cut(s) 47, 72, 150
Hpy188III TCNNGA 3 cut(s) 209, 687, 847
HpyAV CCTTC 1 cut(s) 68
HpyCH4III ACNGT 2 cut(s) 184, 904
HpyCH4IV ACGT 3 cut(s) 918, 925, 932
HpyCH4V TGCA 5 cut(s) 511, 601, 652, 780, 900
HpyF10VI GCNNNNNNNGC 4 cut(s) 255, 593, 598, 781
HpyF3I CTNAG 3 cut(s) 68, 387, 747
HpySE526I ACGT 3 cut(s) 918, 925, 932
Hsp92I GRCGYC 1 cut(s) 585
Hsp92II CATG 6 cut(s) 286, 353, 580, 649, 661, 697
HspAI GCGC 2 cut(s) 408, 529
Kpn2I TCCGGA 1 cut(s) 846
Kzo9I GATC 2 cut(s) 519, 633
LmnI GCTCC 2 cut(s) 34, 567
Lsp1109I GCAGC 4 cut(s) 104, 270, 273, 677
LweI GCATC 4 cut(s) 180, 210, 639, 837
MaeI CTAG 2 cut(s) 198, 737
MaeII ACGT 3 cut(s) 918, 925, 932
MalI GATC 2 cut(s) 521, 635
MboI GATC 2 cut(s) 519, 633
MboII GAAGA 1 cut(s) 481
MhlI GDGCHC 1 cut(s) 706
MluCI AATT 3 cut(s) 266, 473, 503
MlyI GAGTC 4 cut(s) 169, 301, 803, 893
MmeI TCCRAC 1 cut(s) 822
MroI TCCGGA 1 cut(s) 846
MseI TTAA 3 cut(s) 312, 371, 506
MspI CCGG 5 cut(s) 125, 235, 434, 614, 847
MspR9I CCNGG 2 cut(s) 162, 235
Mva1269I GAATGC 1 cut(s) 79
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 4 cut(s) 255, 593, 598, 781
NciI CCSGG 1 cut(s) 235
NcoI CCATGG 3 cut(s) 349, 576, 693
NdeII GATC 2 cut(s) 519, 633
NlaIII CATG 6 cut(s) 286, 353, 580, 649, 661, 697
NlaIV GGNNCC 3 cut(s) 30, 347, 691
PcsI WCGNNNNNNNCGW 1 cut(s) 888
PctI GAATGC 1 cut(s) 79
PfeI GAWTC 1 cut(s) 23
PflMI CCANNNNNTGG 1 cut(s) 699
PkrI GCNGC 5 cut(s) 66, 119, 260, 263, 667
PleI GAGTC 4 cut(s) 169, 301, 802, 892
PmaCI CACGTG 1 cut(s) 926
PmlI CACGTG 1 cut(s) 926
PpsI GAGTC 4 cut(s) 169, 301, 802, 892
Ppu21I YACGTR 1 cut(s) 926
PpuMI RGGWCCY 1 cut(s) 222
PshBI ATTAAT 1 cut(s) 506
Psp5II RGGWCCY 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 160
PspCI CACGTG 1 cut(s) 926
PspFI CCCAGC 1 cut(s) 856
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 3 cut(s) 30, 347, 691
PspPI GGNCC 5 cut(s) 4, 164, 222, 863, 954
PspPPI RGGWCCY 1 cut(s) 222
RsaI GTAC 1 cut(s) 854
RsaNI GTAC 1 cut(s) 853
SaqAI TTAA 3 cut(s) 312, 371, 506
SatI GCNGC 5 cut(s) 65, 118, 259, 262, 666
Sau3AI GATC 2 cut(s) 519, 633
Sau96I GGNCC 5 cut(s) 4, 164, 222, 863, 954
SchI GAGTC 4 cut(s) 169, 301, 803, 893
ScrFI CCNGG 2 cut(s) 162, 235
SduI GDGCHC 1 cut(s) 706
SfaNI GCATC 4 cut(s) 180, 210, 639, 837
SinI GGWCC 2 cut(s) 4, 222
SmlI CTYRAG 2 cut(s) 464, 788
SmoI CTYRAG 2 cut(s) 464, 788
Sse9I AATT 3 cut(s) 266, 473, 503
SsiI CCGC 7 cut(s) 33, 64, 108, 335, 343, 365, 581
SspI AATATT 1 cut(s) 620
SspMI CTAG 2 cut(s) 198, 737
StyD4I CCNGG 2 cut(s) 160, 233
StyI CCWWGG 3 cut(s) 349, 576, 693
TaaI ACNGT 2 cut(s) 184, 904
TaiI ACGT 3 cut(s) 921, 928, 935
TaqI TCGA 3 cut(s) 210, 244, 300
TasI AATT 3 cut(s) 266, 473, 503
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 3 cut(s) 312, 371, 506
Tru9I TTAA 3 cut(s) 312, 371, 506
TscAI CASTG 2 cut(s) 187, 909
TseI GCWGC 4 cut(s) 117, 258, 261, 665
TspDTI ATGAA 4 cut(s) 228, 299, 852, 959
TspGWI ACGGA 2 cut(s) 194, 804
TspRI CASTG 2 cut(s) 187, 909
Van91I CCANNNNNTGG 1 cut(s) 699
VpaK11BI GGWCC 2 cut(s) 4, 222
VspI ATTAAT 1 cut(s) 506
XapI RAATTY 1 cut(s) 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XspI CTAG 2 cut(s) 198, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.