FvH4_2g35260
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001321
Physical Location & Seq
Reverse (-)
26026907 .. 26031080
4174 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35260.t1

Sequence Viewer

Length: 3063 bp
ATGGCCAAAATACTCCTACCCCTCTTCTTCTCTATCCTCATCTCCATACCCCTCAACGTAATTTCACAATCCACGGACTCCGACCGGTCGATCCTACTCAAACTGAAGCAGGAATGGGGCGATCCGCCGTCCATCCAGTCCTGGAACTCCTCATCATCACCGTGCGATTGGCCGGAGATTAGCTGCACAGCCGGCGCGGTCACCGGACTCTTACTCGGAGAAAAAAATATCACGGAGGAAATTCCGGCGACTATTTGCGACCTTCGGAACCTCACCGTGCTCAACCTCTCCTGGAATTACATTCCCGGCGAGTTTCCGGTGGTTCTCTACAACTGCTTCAAGCTCCAGTACCTCGACCTGTCTCAAAACTACCTCGTCGGAGAAATTCCCGGCGATATTGACCGGATATCATCTCTGCAGTACTTGGACGTCAGCGGGAACAACTTCTCCGGCGACATTCCAGCGGCGATCGGAAACTTAACGCAGCTGAAGGTTTTGAATCTTAATTTAAATCTTTTCAACGGAACTTTTCCGAGCGATATCGGCAAGTTGTCGAATCTCGAGATCTTAGACATGTCGTTTAACGGTGAGATGATGGCTGCGCAAATCCCAGAGGAGTTTGGAAAGCTGGGGAAGCTGAAGGAGTTCCGGATGAGGCTGTCGAATTTGATCGGTCAAATCCCGGAGACTTTTTCTAATCTTGTGAGCCTCCAGGAGCTTGATCTTGCGATTAACAAATTGGAAGGGAAGATTCCTGATGGCTTGTTTTTGTTGAAGGATTTGAGAATCTTGTTTCTGTTTCACAATAGATTGACCGGAGAGATTCCGGTCACAGTTGGAGCCATGAGTTTGGAGCAAATTGACCTCGCTATGAACAACTTGACCGGCTCAATCCCACCGGATTTCGGCAAGCTGAGCAATCTGACTGTGTTGAATCTGTACACGAATAAGTTGAACGGTGGAATTCCAGCGAGTTTAGGCCTGATTACGACGCTGAAGGGCTTTCGGGTTTTCAAGAACCAGCTGAATGGGACTCTGCCGCCGGAGATGGGCCTTCACTCGAAGCTTGAAGGGTTTGAGGTTTCGGAGAATCAGCTGAGTGGTGCACTGCCGGAGCATCTGTGCAGCAAAGGCTTGTTGCAAGGAGCTATTGCATTCTCTAACAAACTTAGCGGAGAGTTGCCTAAAGGGCTTGGGAATTGTACTGCTTTGCGTTCGGTGCAACTCTACAACAACAGTTTTTCCGGTGAGCTACCCAAGGGGCTTTGGACTTCACTGAATCTTTCGACGCTGATGATTAGCAACAATTCTTTTTCGGGGGAGCTTCCGAGGACAAGGCTGGCTTGGAATCTGTCTAGGCTGGAAATCAGCAACAACAGATTTTCTGGTGAAATCCCGGTTCAAGTTTCATCTTGGCAGACTTTGGTTGTTTTCAAGGCAAGTGGGAATCTGTTTACAGGGAAAATCCCAGTAGAATTGACAAGTCTTTCTAAGCTGAACACTTTGTCACTCGATGGGAATAGATTTTCAGGTGAATTACCATCAGAGATCATTGCGTGGACATCGTTGACTACTCTGGATCTCTCAAGAAATGAGCTTTCAGGTTACATTCCAACAGCTATTGGTTCTTTGCCAGACCTCCTTTACTTGGATTTGTCAGGAAACAAATTTTCAGGTCAAATCCCATCTGAATTAGGTCACTTGAGGCTCAACTCCCTCAACTTGTCTTCAAATGAGCTTTCCGGGAAGATCCCTGATGTGTTTGATAACCTTGTTTATGAAAACAGTTTCCTGAACAATTCAAATCTATGTGCCAATAGTCCGATTCTAAACCTTCCCAATTGCTACACCAAACTCCATAGTTCCCACAAGTTATCCTCCAAAGTCCTTGCCATGATCTTAGTTCTTTCCATTGTTGTGTTTATAGTTGCTGTTCTATTGACATTTTTTGTTGTAAGAGACCACCGGAGGAAGAAGCGCGGCCATGACTTGGCGACATGGAAGCTCACCTCATTCCAGAGGTTGGATTTCACCGAGTTCAATGTCTTGGCAAACTTGACTGATACTAATCTCATTGGGAGTGGAGGCTCAGGGAAGGTTTATCGAGTTAGTACTAACTGCCCAAGTGAATTTGTCGCGGTGAAGAGGATTTGGAACAGCAAGGAACTGGATCAGAGGCTCGAGAAAGAATTCAATGCTGAGGTTGAGATACTGGGCAGCATTCGTCATTCGAATATTGTCAAGTTGCTGTGCTGCATTTCTAGTGAGAATTCAAAGCTTTTAGTGTATGAATACATGGAGAATCATAGCCTAGATAAGTGGCTTCATGGAAAGAAGACAAAGACAAAGCAAATGGCAGGGATGACTTTGGCTCGTCATGTTGTTTTGGACTGGCCAAAGAGATTGGAGATTGCAATAGGATCTGCACAAGGCTTGTACTACATGCACCATGAGTGCTCTCCCCCGGTAATTCATAGAGATGTCAAGTCCAGCAATATTTTGTTGGACTCAAAGTTCAAAGCTCGCATTGCAGATTTCGGGCTGGCTAAGATCTTGGCCAAGCATGGAGAAGGAGAACCCCACACTATGTCTGTTATTGCAGGCTCTTTTGGTTATATTGCCCCAGAATATGCTTATACGATGAAAATCAATGAAAAGACAGATGTTTTCAGCTTTGGAGTAGTACTTCTAGAGCTAACAACAGGCAGAGAACCTAACAATGGAGGTGAATACACCAACCTAGCAGAATGGGCATGGCAGCAATATGGTGAAGGAAAGAACATTGATGAGGCCCTGGATGAGGATGTAAAGAAAACATGCTACTCGGAAGAGATGGCTACTGTGTTTAAACTGGGACTCATCTGCACTAGCACACTACCTTCAACTCGGCCTTCCATGAAGGAAGTTCTCCACATTCTTCGCGGTTATGGTTCTTCAGATGGTTATGATATCAAAAAGGTGGGGAGTGAGTTTGACATTGCACCTCTCCTTAACACGGCGTCTTACCTTTCCAGTTACAAACGCAGTAAAAAGAAAGACGATAGCATAGTTTTCAGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1021

Amino Acids

112.84

Weight (kDa)

6.28

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 63 3e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 89 - 149 7e-08 Leucine rich repeat
LRR_14 PF23598 110 - 221 1.7e-13 Leucine-rich repeat region
LRR_8 PF13855 138 - 195 5.7e-06 Leucine rich repeat
LRR_8 PF13855 228 - 271 9.9e-06 Leucine rich repeat
LRR_14 PF23598 468 - 569 3.1e-06 Leucine-rich repeat region
LRR_8 PF13855 520 - 557 2.5e-06 Leucine rich repeat
Pkinase PF00069 689 - 971 1.2e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 690 - 972 3.8e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 432
Acc16I TGCGCA 1 cut(s) 603
AccB7I CCANNNNNTGG 2 cut(s) 1990, 2023
AccII CGCG 4 cut(s) 197, 1980, 2138, 2922
AccIII TCCGGA 1 cut(s) 648
AciI CCGC 9 cut(s) 125, 197, 435, 464, 1040, 1173, 1980, 2138, 2922
AclWI GGATC 6 cut(s) 85, 116, 1587, 1744, 2178, 2428
AcoI YGGCCR 5 cut(s) 3, 170, 1981, 2393, 2556
AcsI RAATTY 8 cut(s) 240, 384, 664, 963, 1667, 2129, 2189, 2269
AcuI CTGAAG 5 cut(s) 125, 509, 659, 1016, 2919
AcyI GRCGYC 2 cut(s) 429, 2999
AfaI GTAC 7 cut(s) 350, 422, 941, 1204, 2113, 2438, 2685
AfiI CCNNNNNNNGG 8 cut(s) 905, 1049, 1648, 1990, 2023, 2720, 2800, 2995
AflIII ACRYGT 1 cut(s) 573
AgeI ACCGGT 1 cut(s) 84
AjnI CCWGG 4 cut(s) 140, 290, 711, 2793
AloI GAACNNNNNNTCC 4 cut(s) 431, 463, 2498, 2530
Alw21I GWGCWC 3 cut(s) 282, 1108, 2459
Alw26I GTCTC 3 cut(s) 366, 680, 1953
Alw44I GTGCAC 1 cut(s) 1104
AlwI GGATC 6 cut(s) 85, 116, 1587, 1744, 2178, 2428
Ama87I CYCGRG 2 cut(s) 560, 2180
Aor13HI TCCGGA 1 cut(s) 648
AoxI GGCC 9 cut(s) 3, 170, 979, 1051, 1981, 2393, 2556, 2790, 2888
ApaLI GTGCAC 1 cut(s) 1104
ApeKI GCWGC 7 cut(s) 183, 484, 599, 1125, 2217, 2253, 2758
ApoI RAATTY 8 cut(s) 240, 384, 664, 963, 1667, 2129, 2189, 2269
AsiGI ACCGGT 1 cut(s) 84
Asp700I GAANNNNTTC 3 cut(s) 443, 644, 2189
AspLEI GCGC 3 cut(s) 197, 604, 1980
AspS9I GGNCC 2 cut(s) 1051, 2791
AsuC2I CCSGG 6 cut(s) 306, 390, 683, 1397, 1744, 2465
AsuII TTCGAA 1 cut(s) 2231
AvaI CYCGRG 2 cut(s) 560, 2180
BaeGI GKGCMC 1 cut(s) 1108
BalI TGGCCA 3 cut(s) 5, 2395, 2558
BbsI GAAGAC 2 cut(s) 1719, 2342
Bbv12I GWGCWC 3 cut(s) 282, 1108, 2459
BbvCI CCTCAGC 1 cut(s) 2199
BbvI GCAGC 7 cut(s) 170, 496, 586, 1137, 2229, 2240, 2770
BccI CCATC 9 cut(s) 140, 589, 752, 1042, 1508, 1549, 1693, 2827, 2933
BceAI ACGGC 2 cut(s) 112, 3012
BciT130I CCWGG 4 cut(s) 142, 292, 713, 2795
BcnI CCSGG 6 cut(s) 306, 390, 683, 1397, 1744, 2465
BcoDI GTCTC 3 cut(s) 366, 680, 1953
BfaI CTAG 6 cut(s) 1356, 2262, 2312, 2690, 2741, 2868
BfmI CTRYAG 1 cut(s) 416
BglI GCCNNNNNGGC 1 cut(s) 1189
BglII AGATCT 2 cut(s) 564, 2550
BlpI GCTNAGC 1 cut(s) 914
BmcAI AGTACT 3 cut(s) 422, 2113, 2685
BmeT110I CYCGRG 2 cut(s) 560, 2180
BmgT120I GGNCC 2 cut(s) 1051, 2791
BmiI GGNNCC 2 cut(s) 269, 841
BmrI ACTGGG 3 cut(s) 1463, 2222, 2861
BmsI GCATC 1 cut(s) 1126
BmuI ACTGGG 3 cut(s) 1463, 2222, 2861
BpiI GAAGAC 2 cut(s) 1719, 2342
BpmI CTGGAG 2 cut(s) 329, 695
Bpu10I CCTNAGC 2 cut(s) 2089, 2199
Bpu1102I GCTNAGC 1 cut(s) 914
Bpu14I TTCGAA 1 cut(s) 2231
BpuEI CTTGAG 2 cut(s) 1570, 1723
BpuMI CCSGG 6 cut(s) 306, 390, 683, 1397, 1744, 2465
BsaBI GATNNNNATC 2 cut(s) 2067, 2645
BsaHI GRCGYC 2 cut(s) 429, 2999
BsaI GGTCTC 1 cut(s) 1953
BsaJI CCNNGG 5 cut(s) 72, 1257, 1328, 2463, 2793
BsaXI ACNNNNNCTCC 4 cut(s) 431, 461, 1106, 1136
Bsc4I CCNNNNNNNGG 8 cut(s) 905, 1049, 1648, 1990, 2023, 2720, 2800, 2995
Bse118I RCCGGY 3 cut(s) 84, 191, 884
Bse1I ACTGG 8 cut(s) 136, 346, 1469, 2172, 2217, 2396, 2856, 3012
Bse3DI GCAATG 3 cut(s) 1551, 2526, 2976
Bse8I GATNNNNATC 2 cut(s) 2067, 2645
BseAI TCCGGA 1 cut(s) 648
BseBI CCWGG 4 cut(s) 142, 292, 713, 2795
BseDI CCNNGG 5 cut(s) 72, 1257, 1328, 2463, 2793
BseGI GGATG 5 cut(s) 132, 657, 2367, 2803, 2809
BseJI GATNNNNATC 2 cut(s) 2067, 2645
BseLI CCNNNNNNNGG 8 cut(s) 905, 1049, 1648, 1990, 2023, 2720, 2800, 2995
BseMI GCAATG 3 cut(s) 1551, 2526, 2976
BseMII CTCAG 4 cut(s) 905, 1088, 2103, 2190
BseNI ACTGG 8 cut(s) 136, 346, 1469, 2172, 2217, 2396, 2856, 3012
BseRI GAGGAG 2 cut(s) 139, 629
BseSI GKGCMC 1 cut(s) 1108
BseXI GCAGC 7 cut(s) 170, 496, 586, 1137, 2229, 2240, 2770
BseYI CCCAGC 1 cut(s) 628
BsgI GTGCAG 4 cut(s) 169, 1144, 2409, 2848
Bsh1236I CGCG 4 cut(s) 197, 1980, 2138, 2922
Bsh1285I CGRYCG 3 cut(s) 85, 89, 471
BshFI GGCC 9 cut(s) 5, 172, 981, 1053, 1983, 2395, 2558, 2792, 2890
BshTI ACCGGT 1 cut(s) 84
BsiEI CGRYCG 3 cut(s) 85, 89, 471
BsiHKAI GWGCWC 3 cut(s) 282, 1108, 2459
BsiHKCI CYCGRG 2 cut(s) 560, 2180
BslFI GGGAC 2 cut(s) 1045, 2868
BslI CCNNNNNNNGG 8 cut(s) 905, 1049, 1648, 1990, 2023, 2720, 2800, 2995
BsmAI GTCTC 3 cut(s) 366, 680, 1953
BsmFI GGGAC 2 cut(s) 1045, 2868
BsmI GAATGC 2 cut(s) 1154, 2220
BsnI GGCC 9 cut(s) 5, 172, 981, 1053, 1983, 2395, 2558, 2792, 2890
Bso31I GGTCTC 1 cut(s) 1953
BsoBI CYCGRG 2 cut(s) 560, 2180
Bsp119I TTCGAA 1 cut(s) 2231
Bsp1286I GDGCHC 3 cut(s) 282, 1108, 2459
Bsp13I TCCGGA 1 cut(s) 648
Bsp1407I TGTACA 1 cut(s) 939
Bsp1720I GCTNAGC 1 cut(s) 914
BspACI CCGC 9 cut(s) 125, 197, 435, 464, 1040, 1173, 1980, 2138, 2922
BspANI GGCC 9 cut(s) 5, 172, 981, 1053, 1983, 2395, 2558, 2792, 2890
BspCNI CTCAG 4 cut(s) 906, 1089, 2102, 2191
BspEI TCCGGA 1 cut(s) 648
BspFNI CGCG 4 cut(s) 197, 1980, 2138, 2922
BspLI GGNNCC 2 cut(s) 269, 841
BspMAI CTGCAG 1 cut(s) 420
BspPI GGATC 6 cut(s) 85, 116, 1587, 1744, 2178, 2428
BspT104I TTCGAA 1 cut(s) 2231
BspTNI GGTCTC 1 cut(s) 1953
BsrDI GCAATG 3 cut(s) 1551, 2526, 2976
BsrFI RCCGGY 3 cut(s) 84, 191, 884
BsrGI TGTACA 1 cut(s) 939
BsrI ACTGG 8 cut(s) 136, 346, 1469, 2172, 2217, 2396, 2856, 3012
BssAI RCCGGY 3 cut(s) 84, 191, 884
BssECI CCNNGG 5 cut(s) 72, 1257, 1328, 2463, 2793
BssNI GRCGYC 2 cut(s) 429, 2999
BssT1I CCWWGG 1 cut(s) 1257
Bst2UI CCWGG 4 cut(s) 142, 292, 713, 2795
Bst4CI ACNGT 9 cut(s) 162, 277, 587, 835, 928, 959, 1238, 1787, 2842
Bst6I CTCTTC 3 cut(s) 29, 2138, 2823
BstACI GRCGYC 2 cut(s) 429, 2999
BstAUI TGTACA 1 cut(s) 939
BstBI TTCGAA 1 cut(s) 2231
BstC8I GCNNGC 6 cut(s) 193, 911, 1341, 2524, 2544, 2602
BstDEI CTNAG 9 cut(s) 568, 914, 1097, 1169, 1491, 1900, 2089, 2199, 2547
BstDSI CCRYGG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 199
BstENI CCTNNNNNAGG 1 cut(s) 2798
BstF5I GGATG 5 cut(s) 132, 657, 2367, 2803, 2809
BstFNI CGCG 4 cut(s) 197, 1980, 2138, 2922
BstHHI GCGC 3 cut(s) 197, 604, 1980
BstMAI GTCTC 3 cut(s) 366, 680, 1953
BstMCI CGRYCG 3 cut(s) 85, 89, 471
BstMWI GCNNNNNNNGC 9 cut(s) 192, 543, 634, 915, 1131, 1189, 1219, 2528, 2750
BstNI CCWGG 4 cut(s) 142, 292, 713, 2795
BstNSI RCATGY 3 cut(s) 577, 2446, 2820
BstPI GGTNACC 1 cut(s) 199
BstSFI CTRYAG 1 cut(s) 416
BstSLI GKGCMC 1 cut(s) 1108
BstUI CGCG 4 cut(s) 197, 1980, 2138, 2922
BstV1I GCAGC 7 cut(s) 170, 496, 586, 1137, 2229, 2240, 2770
BstV2I GAAGAC 2 cut(s) 1719, 2342
BstX2I RGATCY 5 cut(s) 564, 1579, 1749, 2420, 2550
BstXI CCANNNNNNTGG 2 cut(s) 850, 1028
BstYI RGATCY 5 cut(s) 564, 1579, 1749, 2420, 2550
BsuRI GGCC 9 cut(s) 5, 172, 981, 1053, 1983, 2395, 2558, 2792, 2890
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 5 cut(s) 132, 657, 2367, 2803, 2809
BtsI GCAGTG 1 cut(s) 1106
BtsIMutI CAGTG 3 cut(s) 1106, 1274, 3061
Cac8I GCNNGC 6 cut(s) 193, 911, 1341, 2524, 2544, 2602
CfoI GCGC 3 cut(s) 197, 604, 1980
Cfr10I RCCGGY 3 cut(s) 84, 191, 884
Cfr13I GGNCC 2 cut(s) 1051, 2791
CseI GACGC 3 cut(s) 1000, 1297, 2988
Csp6I GTAC 7 cut(s) 349, 421, 940, 1203, 2112, 2437, 2684
CspAI ACCGGT 1 cut(s) 84
CspCI CAANNNNNGTGG 2 cut(s) 1423, 1458
CviQI GTAC 7 cut(s) 349, 421, 940, 1203, 2112, 2437, 2684
DdeI CTNAG 9 cut(s) 568, 914, 1097, 1169, 1491, 1900, 2089, 2199, 2547
DraI TTTAAA 2 cut(s) 510, 2848
EaeI YGGCCR 5 cut(s) 3, 170, 1981, 2393, 2556
Eam1104I CTCTTC 3 cut(s) 29, 2138, 2823
EarI CTCTTC 3 cut(s) 29, 2138, 2823
EciI GGCGGA 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 1257
Eco147I AGGCCT 1 cut(s) 981
Eco31I GGTCTC 1 cut(s) 1953
Eco32I GATATC 3 cut(s) 408, 541, 2950
Eco57I CTGAAG 5 cut(s) 125, 509, 659, 1016, 2919
Eco88I CYCGRG 2 cut(s) 560, 2180
Eco91I GGTNACC 1 cut(s) 199
EcoNI CCTNNNNNAGG 1 cut(s) 2798
EcoO109I RGGNCCY 1 cut(s) 2791
EcoO65I GGTNACC 1 cut(s) 199
EcoRI GAATTC 3 cut(s) 963, 2189, 2269
EcoRII CCWGG 4 cut(s) 140, 290, 711, 2793
EcoRV GATATC 3 cut(s) 408, 541, 2950
EcoT14I CCWWGG 1 cut(s) 1257
ErhI CCWWGG 1 cut(s) 1257
FalI AAGNNNNNCTT 2 cut(s) 1327, 1359
FaqI GGGAC 2 cut(s) 1045, 2868
FauI CCCGC 1 cut(s) 428
FokI GGATG 5 cut(s) 119, 664, 2374, 2810, 2816
FspBI CTAG 6 cut(s) 1356, 2262, 2312, 2690, 2741, 2868
FspI TGCGCA 1 cut(s) 603
GlaI GCGC 3 cut(s) 196, 603, 1979
GsaI CCCAGC 1 cut(s) 632
GsuI CTGGAG 2 cut(s) 329, 695
HaeIII GGCC 9 cut(s) 5, 172, 981, 1053, 1983, 2395, 2558, 2792, 2890
HgaI GACGC 3 cut(s) 1000, 1297, 2988
HhaI GCGC 3 cut(s) 197, 604, 1980
Hin1I GRCGYC 2 cut(s) 429, 2999
Hin6I GCGC 3 cut(s) 195, 602, 1978
HinP1I GCGC 3 cut(s) 195, 602, 1978
HincII GTYRAC 1 cut(s) 1569
HindII GTYRAC 1 cut(s) 1569
HindIII AAGCTT 2 cut(s) 1064, 2276
Hpy166II GTNNAC 5 cut(s) 942, 1106, 1455, 1560, 1569
Hpy8I GTNNAC 5 cut(s) 942, 1106, 1455, 1560, 1569
Hpy99I CGWCG 3 cut(s) 380, 994, 1291
HpyCH4III ACNGT 9 cut(s) 162, 277, 587, 835, 928, 959, 1238, 1787, 2842
HpyCH4IV ACGT 2 cut(s) 57, 429
HpyF10VI GCNNNNNNNGC 9 cut(s) 192, 543, 634, 915, 1131, 1189, 1219, 2528, 2750
HpyF3I CTNAG 9 cut(s) 568, 914, 1097, 1169, 1491, 1900, 2089, 2199, 2547
HpySE526I ACGT 2 cut(s) 57, 429
Hsp92I GRCGYC 2 cut(s) 429, 2999
HspAI GCGC 3 cut(s) 195, 602, 1978
Kpn2I TCCGGA 1 cut(s) 648
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
LmnI GCTCC 7 cut(s) 348, 715, 839, 853, 1114, 1145, 1321
Lsp1109I GCAGC 7 cut(s) 170, 496, 586, 1137, 2229, 2240, 2770
LweI GCATC 1 cut(s) 1126
MaeI CTAG 6 cut(s) 1356, 2262, 2312, 2690, 2741, 2868
MaeII ACGT 2 cut(s) 57, 429
MaeIII GTNAC 6 cut(s) 199, 829, 1506, 1604, 1697, 3014
MfeI CAATTG 1 cut(s) 1840
MflI RGATCY 5 cut(s) 564, 1579, 1749, 2420, 2550
MhlI GDGCHC 3 cut(s) 282, 1108, 2459
MlsI TGGCCA 3 cut(s) 5, 2395, 2558
MluNI TGGCCA 3 cut(s) 5, 2395, 2558
MlyI GAGTC 5 cut(s) 71, 201, 1027, 2501, 2850
MmeI TCCRAC 6 cut(s) 105, 358, 817, 1637, 2004, 2484
Mox20I TGGCCA 3 cut(s) 5, 2395, 2558
MroI TCCGGA 1 cut(s) 648
MroNI GCCGGC 1 cut(s) 191
MroXI GAANNNNTTC 3 cut(s) 443, 644, 2189
MscI TGGCCA 3 cut(s) 5, 2395, 2558
MseI TTAA 7 cut(s) 479, 504, 509, 582, 732, 2847, 2991
MslI CAYNNNNRTG 3 cut(s) 160, 1916, 2478
Msp20I TGGCCA 3 cut(s) 5, 2395, 2558
MspA1I CMGCKG 5 cut(s) 435, 464, 487, 1024, 1096
MssI GTTTAAAC 1 cut(s) 2848
MunI CAATTG 1 cut(s) 1840
Mva1269I GAATGC 2 cut(s) 1154, 2220
MvaI CCWGG 4 cut(s) 142, 292, 713, 2795
MvnI CGCG 4 cut(s) 197, 1980, 2138, 2922
MwoI GCNNNNNNNGC 9 cut(s) 192, 543, 634, 915, 1131, 1189, 1219, 2528, 2750
NaeI GCCGGC 1 cut(s) 193
NciI CCSGG 6 cut(s) 306, 390, 683, 1397, 1744, 2465
NgoMIV GCCGGC 1 cut(s) 191
NlaIV GGNNCC 2 cut(s) 269, 841
NmeAIII GCCGAG 1 cut(s) 2866
NmuCI GTSAC 4 cut(s) 199, 829, 1506, 1697
NsbI TGCGCA 1 cut(s) 603
NspI RCATGY 3 cut(s) 577, 2446, 2820
NspV TTCGAA 1 cut(s) 2231
PaeR7I CTCGAG 2 cut(s) 560, 2180
PceI AGGCCT 1 cut(s) 981
PciI ACATGT 1 cut(s) 573
PctI GAATGC 2 cut(s) 1154, 2220
PdiI GCCGGC 1 cut(s) 193
PdmI GAANNNNTTC 3 cut(s) 443, 644, 2189
PflMI CCANNNNNTGG 2 cut(s) 1990, 2023
PfoI TCCNGGA 5 cut(s) 140, 290, 681, 711, 1742
PinAI ACCGGT 1 cut(s) 84
Ple19I CGATCG 1 cut(s) 471
PleI GAGTC 5 cut(s) 71, 201, 1027, 2501, 2850
PmeI GTTTAAAC 1 cut(s) 2848
PpsI GAGTC 5 cut(s) 71, 201, 1027, 2501, 2850
PscI ACATGT 1 cut(s) 573
Psp6I CCWGG 4 cut(s) 140, 290, 711, 2793
PspEI GGTNACC 1 cut(s) 199
PspFI CCCAGC 1 cut(s) 628
PspGI CCWGG 4 cut(s) 140, 290, 711, 2793
PspN4I GGNNCC 2 cut(s) 269, 841
PspPI GGNCC 2 cut(s) 1051, 2791
PstI CTGCAG 1 cut(s) 420
PsuI RGATCY 5 cut(s) 564, 1579, 1749, 2420, 2550
PvuI CGATCG 1 cut(s) 471
PvuII CAGCTG 3 cut(s) 487, 1024, 1096
RsaI GTAC 7 cut(s) 350, 422, 941, 1204, 2113, 2438, 2685
RsaNI GTAC 7 cut(s) 349, 421, 940, 1203, 2112, 2437, 2684
RseI CAYNNNNRTG 3 cut(s) 160, 1916, 2478
SaqAI TTAA 7 cut(s) 479, 504, 509, 582, 732, 2847, 2991
Sau96I GGNCC 2 cut(s) 1051, 2791
ScaI AGTACT 3 cut(s) 422, 2113, 2685
SchI GAGTC 5 cut(s) 71, 201, 1027, 2501, 2850
SduI GDGCHC 3 cut(s) 282, 1108, 2459
SfaNI GCATC 1 cut(s) 1126
SfcI CTRYAG 1 cut(s) 416
Sfr274I CTCGAG 2 cut(s) 560, 2180
SfuI TTCGAA 1 cut(s) 2231
SlaI CTCGAG 2 cut(s) 560, 2180
SmiI ATTTAAAT 1 cut(s) 510
SmiMI CAYNNNNRTG 3 cut(s) 160, 1916, 2478
SmlI CTYRAG 4 cut(s) 560, 1585, 1702, 2180
SmoI CTYRAG 4 cut(s) 560, 1585, 1702, 2180
SseBI AGGCCT 1 cut(s) 981
SsiI CCGC 9 cut(s) 125, 197, 435, 464, 1040, 1173, 1980, 2138, 2922
SspI AATATT 2 cut(s) 2236, 2497
SspMI CTAG 6 cut(s) 1356, 2262, 2312, 2690, 2741, 2868
StuI AGGCCT 1 cut(s) 981
StyI CCWWGG 1 cut(s) 1257
SwaI ATTTAAAT 1 cut(s) 510
TaaI ACNGT 9 cut(s) 162, 277, 587, 835, 928, 959, 1238, 1787, 2842
TaiI ACGT 2 cut(s) 60, 432
TaqII GACCGA 1 cut(s) 662
TatI WGTACW 6 cut(s) 420, 939, 1202, 2111, 2436, 2683
TauI GCSGC 3 cut(s) 467, 1042, 1983
Tru1I TTAA 7 cut(s) 479, 504, 509, 582, 732, 2847, 2991
Tru9I TTAA 7 cut(s) 479, 504, 509, 582, 732, 2847, 2991
TscAI CASTG 3 cut(s) 1113, 1281, 3061
TseFI GTSAC 4 cut(s) 199, 829, 1506, 1697
TseI GCWGC 7 cut(s) 183, 484, 599, 1125, 2217, 2253, 2758
Tsp45I GTSAC 4 cut(s) 199, 829, 1506, 1697
TspDTI ATGAA 9 cut(s) 887, 1398, 1794, 2304, 2315, 2462, 2657, 2667, 2912
TspGWI ACGGA 3 cut(s) 89, 248, 537
TspRI CASTG 3 cut(s) 1113, 1281, 3061
Van91I CCANNNNNTGG 2 cut(s) 1990, 2023
VneI GTGCAC 1 cut(s) 1104
XagI CCTNNNNNAGG 1 cut(s) 2798
XapI RAATTY 8 cut(s) 240, 384, 664, 963, 1667, 2129, 2189, 2269
XbaI TCTAGA 1 cut(s) 2689
XceI RCATGY 3 cut(s) 577, 2446, 2820
XhoI CTCGAG 2 cut(s) 560, 2180
XmnI GAANNNNTTC 3 cut(s) 443, 644, 2189
XspI CTAG 6 cut(s) 1356, 2262, 2312, 2690, 2741, 2868
ZraI GACGTC 1 cut(s) 430
ZrmI AGTACT 3 cut(s) 422, 2113, 2685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.