pycom08g09200
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001321
Physical Location & Seq
Reverse (-)
7439184 .. 7441723
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09200.1

Sequence Viewer

Length: 2463 bp
ATGGTCACTGGGCTCTCCCTCCCCGATGTAAACATCACGGAGAGAATCCCGGAGGCCGTCTGCGAGCTCCCACGCCTAGCAGAGCTCCACCTCGCCTGGAATTACATTCCAGGCGAGTTCCCGAAATTTCTCTACAACTGCTCGAATCTCGAAGTCCTTGACCTCTCGCAGAACTACTTTGTCGGCCCAATTCCCCCCGATATCTACCGGATGTCGCCGTCTCTTAAGTACTTGGACCTCGGAGGAAACAATTTTTCCGGCAACATTCCTGCGGCCATCGGCCGGCTGACGGAGCTGAGGACACTCAAGCTTTATTCGAATCTGTTCAACGGGAGTGTTCCGCCGGAGATCGATAACTTGTCAAATCTCGAAATATTCGAGATGCCTTATAACGGAAACTTGGCTGCGGCCAGCATCCCAACGGAGTTCGGGAATTTGAAGAAGCTGAAGAGCTTGTGGATGACGGACACGAACTTGATCGGTCATGCAGAGATTCCGGAGAGTTTTTCAGGTCTCTCGAGCCTCGAGAACTTGAATCTTGCGAGGAACAATCTGGAAGGGAAGATTCCAGGTGGGTTGTTTTTGTTGAAGAATCTGAGCGACTTGTTACTCTTTAACAACAAATTTTCAGGGGAGATTCCACTGGCTGTCGAGGCGTTGAATTTATCTCAGATTGATCTGTCTACGAACAATTTGAGCGGCTCAATCCCACAAGATTTTGGGAAGATAAAGAATCTGACTGTTTTGAATCTTTTTTCGAATCAATTAACGGGTGGGATTCCGGAGAGTTTAGGCCTAATTCCGGCGATGAAGGTATTTCAGGTCTTTCAGAACATGTTGAACGGGACCTTGCCGCCGGAATTAGGCCTTCACTCGAAGCTCGAAGCTTTTGATGTCTCCGGGAATCAGCTGAGCGGCTCTCTGCCGGAACAATTATGCAGTGGAGGGGTTTTGCAAAGAGCTGTTGCGTTCTCTAATAATCTTACCGGAGAGTTGCCCAAAGGGCTTGGAAATTGTGATTCTTTGCGGACGCTGAAGGTCTACCACAACCGTTTTTCGGGTGAGGTTCCTTTCGGAGTTTGGACCGGACTAACTCTGTCGAGTTTGATGCTGAGTGACAATTTGTTTTCGGGTCAGCTTCCCGCCAGCAAGCTGGCTTGGAATCTGTCCAGGCTGGAAATCAGCAACAACAGATTTTCCAGTGAGATTCCGGTTCAAGTTTCGTCCTGGGAACGTTTGGTTGTTTTTAAGGCGAGCGGAAATCTGTTTTCGGGGAAAATCCCAGTTGAACTGACTAGTCTTTCTCAGCTGAACACTCTGCTGCTTGACGGGAATCAATTTTCGGGCGAATTACCATCTCAGATCATCTCATGGGAATCCTTGAATACTTTGAACCTCTCGAGAAATGCGTTTTCGGGCCACATTCCAGCAGCCATTGGTTCTTTGCCTAACCTGCTATACTTGGACTTGTCCGAGAACCAATTCACAGGCGAAATCCCAGCTGAGTTCGGCAACTTGATTCTCAGTTCTCTTAACTTGTCTTCCAACAAGCTTTCCGGCAAAATCCCAGATGTGTTTGATAATCTCGCGTATGAAAACAGTTTCTTGAACAATTCGAATCTTTGCGCCAATAGTCCAGTTCTTAACCTTCCCGGTTGTTACACCAAAGTCCGTGTCTCCCACAAGCTGTCCTCGAAAGTCCTTGCCATGATTCTAGTCCCCATTGCGGTGTTCCTTGTTACTGTTTTATTGACCTTTTTCGTGGTCAGAGACTACTGGAGGAGAAAGCGCGGTCAGGACTTGGCAACATGGAAGCTCACCTCATTCCACAGATTGAATCTCACAGAGTTCAACGTGTTGGCGAGTTTGACGGATACCAATCTCATTGGAAGTGGGGGTTCAGGGAATGTTTATCAGGTTAGCACCAACTGCCCGGGTGAATATGTCGCGGTGAAAAAGATTTGTAACACCAACAGATTGGATGAGAGGCTTGAGAAAGAATTCATTGCTGAGGTTGAGATACTGGGGACAATTCGCCATTCGAACATTGTTAAGTTGTGGTGCTGCATTTCAAGTGACAATTCAAAGCTTCTTGTGTACGAGTACATGGCAAATCAGAGCCTCGATAAGTGGCTACATGGGAAGACGAGAAGACCAGCATCAGGCCTGGGTGTGGCTAATCATATTGTTTTGGACTGGCCGACGAGGTTGCAGATTGCGATAGGGGCTGCACAAGGCTTGTGTTACATGCACCATGACTGCTCTCCGCCGATAATTCATCGTGATGTGAAGTCCAGCAATATCTTGTTGGATTCTGAATTCAAGGCTCGAATAGCAGATTTTGGACTAGCAAAGATTTTTTCCAAGGACGGATCGATCATCACACAATGTCCGCTATTGCAGGCTCCTTCGGCTACATGGCACCAGAGTATTCTTATACAACGAAAATCAACGAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

821

Amino Acids

90.23

Weight (kDa)

6.56

Isoelectric Point (pI)

33.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 24 - 85 8.3e-07 Leucine rich repeat
LRR_14 PF23598 46 - 156 6.7e-07 Leucine-rich repeat region
LRR_8 PF13855 48 - 108 1.7e-06 Leucine rich repeat
Pkinase PF00069 626 - 792 1.2e-29 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 627 - 790 2.5e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 390
Acc36I ACCTGC 1 cut(s) 1461
AccB1I GGYRCC 1 cut(s) 2421
AccBSI CCGCTC 3 cut(s) 699, 915, 1257
AccI GTMKAC 2 cut(s) 683, 1041
AccII CGCG 3 cut(s) 1589, 1791, 1949
AccIII TCCGGA 2 cut(s) 496, 781
AclI AACGTT 1 cut(s) 1234
AclWI GGATC 1 cut(s) 2380
AcoI YGGCCR 4 cut(s) 273, 280, 408, 2198
AcsI RAATTY 6 cut(s) 125, 433, 623, 661, 2000, 2318
AcuI CTGAAG 2 cut(s) 467, 1055
AfaI GTAC 3 cut(s) 230, 2099, 2105
AfiI CCNNNNNNNGG 9 cut(s) 282, 289, 392, 802, 863, 1057, 1726, 2162, 2173
AflII CTTAAG 1 cut(s) 224
AflIII ACRYGT 2 cut(s) 834, 1854
AhlI ACTAGT 1 cut(s) 1295
AjnI CCWGG 6 cut(s) 95, 109, 568, 1169, 1226, 2166
Alw21I GWGCWC 2 cut(s) 69, 87
Alw26I GTCTC 5 cut(s) 225, 518, 901, 1681, 1764
AlwI GGATC 1 cut(s) 2380
Ama87I CYCGRG 4 cut(s) 517, 524, 1399, 1933
Aor13HI TCCGGA 2 cut(s) 496, 781
ApeKI GCWGC 5 cut(s) 404, 1321, 1430, 2064, 2228
ApoI RAATTY 6 cut(s) 125, 433, 623, 661, 2000, 2318
ArsI GACNNNNNNTTYG 2 cut(s) 1659, 1691
Asp700I GAANNNNTTC 3 cut(s) 323, 1481, 2000
AspLEI GCGC 2 cut(s) 1628, 1791
AspS9I GGNCC 5 cut(s) 185, 235, 846, 1083, 1417
AsuC2I CCSGG 5 cut(s) 50, 901, 1653, 1934, 1935
AsuHPI GGTGA 4 cut(s) 1073, 1810, 1949, 1963
AsuII TTCGAA 4 cut(s) 317, 758, 1616, 2042
AvaI CYCGRG 4 cut(s) 517, 524, 1399, 1933
AvaII GGWCC 3 cut(s) 235, 846, 1083
BanI GGYRCC 1 cut(s) 2421
BanII GRGCYC 3 cut(s) 15, 69, 87
BbsI GAAGAC 3 cut(s) 1533, 2150, 2158
Bbv12I GWGCWC 2 cut(s) 69, 87
BbvCI CCTCAGC 2 cut(s) 296, 2010
BbvI GCAGC 5 cut(s) 391, 1308, 1442, 2051, 2215
BccI CCATC 2 cut(s) 284, 1363
BceAI ACGGC 2 cut(s) 41, 202
BcgI CGANNNNNNTGC 4 cut(s) 1090, 1124, 2191, 2225
BciT130I CCWGG 6 cut(s) 97, 111, 570, 1171, 1228, 2168
BciVI GTATCC 1 cut(s) 1867
BcnI CCSGG 5 cut(s) 50, 901, 1653, 1934, 1935
BcoDI GTCTC 5 cut(s) 225, 518, 901, 1681, 1764
BcuI ACTAGT 1 cut(s) 1295
BfaI CTAG 4 cut(s) 77, 1296, 1715, 2348
BfrI CTTAAG 1 cut(s) 224
BfuAI ACCTGC 1 cut(s) 1461
BfuI GTATCC 1 cut(s) 1867
BglI GCCNNNNNGGC 1 cut(s) 1003
BlpI GCTNAGC 1 cut(s) 911
BmcAI AGTACT 1 cut(s) 230
Bme18I GGWCC 3 cut(s) 235, 846, 1083
BmeT110I CYCGRG 4 cut(s) 517, 524, 1399, 1933
BmgT120I GGNCC 5 cut(s) 185, 235, 846, 1083, 1417
BmiI GGNNCC 4 cut(s) 847, 1068, 2406, 2423
BmrI ACTGGG 3 cut(s) 18, 1277, 2033
BmsI GCATC 4 cut(s) 372, 423, 1098, 2168
BmuI ACTGGG 3 cut(s) 18, 1277, 2033
BpiI GAAGAC 3 cut(s) 1533, 2150, 2158
BplI GAGNNNNNCTC 2 cut(s) 904, 936
BpmI CTGGAG 1 cut(s) 1798
Bpu10I CCTNAGC 2 cut(s) 296, 2010
Bpu1102I GCTNAGC 1 cut(s) 911
Bpu14I TTCGAA 4 cut(s) 317, 758, 1616, 2042
BpuEI CTTGAG 2 cut(s) 290, 2012
BpuMI CCSGG 5 cut(s) 50, 901, 1653, 1934, 1935
Bsa29I ATCGAT 2 cut(s) 351, 2375
BsaBI GATNNNNATC 1 cut(s) 1878
BsaI GGTCTC 1 cut(s) 518
BsaJI CCNNGG 5 cut(s) 238, 1227, 1933, 2167, 2364
BsaWI WCCGGW 6 cut(s) 207, 496, 781, 986, 1085, 1210
Bsc4I CCNNNNNNNGG 9 cut(s) 282, 289, 392, 802, 863, 1057, 1726, 2162, 2173
Bse118I RCCGGY 1 cut(s) 282
Bse1I ACTGG 8 cut(s) 13, 648, 1200, 1283, 1637, 1781, 2028, 2201
Bse3DI GCAATG 2 cut(s) 1722, 2004
Bse8I GATNNNNATC 1 cut(s) 1878
BseAI TCCGGA 2 cut(s) 496, 781
BseBI CCWGG 6 cut(s) 97, 111, 570, 1171, 1228, 2168
BseCI ATCGAT 2 cut(s) 351, 2375
BseDI CCNNGG 5 cut(s) 238, 1227, 1933, 2167, 2364
BseGI GGATG 4 cut(s) 216, 414, 465, 1987
BseJI GATNNNNATC 1 cut(s) 1878
BseLI CCNNNNNNNGG 9 cut(s) 282, 289, 392, 802, 863, 1057, 1726, 2162, 2173
BseMI GCAATG 2 cut(s) 1722, 2004
BseNI ACTGG 8 cut(s) 13, 648, 1200, 1283, 1637, 1781, 2028, 2201
BseRI GAGGAG 1 cut(s) 1795
BseX3I CGGCCG 1 cut(s) 280
BseXI GCAGC 5 cut(s) 391, 1308, 1442, 2051, 2215
BseYI CCCAGC 1 cut(s) 1498
BsgI GTGCAG 1 cut(s) 2214
Bsh1236I CGCG 3 cut(s) 1589, 1791, 1949
Bsh1285I CGRYCG 1 cut(s) 283
BshNI GGYRCC 1 cut(s) 2421
BshVI ATCGAT 2 cut(s) 351, 2375
BsiEI CGRYCG 1 cut(s) 283
BsiHKAI GWGCWC 2 cut(s) 69, 87
BsiHKCI CYCGRG 4 cut(s) 517, 524, 1399, 1933
BslFI GGGAC 3 cut(s) 859, 1703, 2041
BslI CCNNNNNNNGG 9 cut(s) 282, 289, 392, 802, 863, 1057, 1726, 2162, 2173
BsmAI GTCTC 5 cut(s) 225, 518, 901, 1681, 1764
BsmBI CGTCTC 1 cut(s) 225
BsmFI GGGAC 3 cut(s) 859, 1703, 2041
Bso31I GGTCTC 1 cut(s) 518
BsoBI CYCGRG 4 cut(s) 517, 524, 1399, 1933
Bsp119I TTCGAA 4 cut(s) 317, 758, 1616, 2042
Bsp1286I GDGCHC 3 cut(s) 15, 69, 87
Bsp13I TCCGGA 2 cut(s) 496, 781
Bsp143I GATC 6 cut(s) 348, 477, 676, 1362, 2372, 2376
Bsp1720I GCTNAGC 1 cut(s) 911
BspDI ATCGAT 2 cut(s) 351, 2375
BspEI TCCGGA 2 cut(s) 496, 781
BspFNI CGCG 3 cut(s) 1589, 1791, 1949
BspLI GGNNCC 4 cut(s) 847, 1068, 2406, 2423
BspMI ACCTGC 1 cut(s) 1461
BspPI GGATC 1 cut(s) 2380
BspQI GCTCTTC 1 cut(s) 443
BspT104I TTCGAA 4 cut(s) 317, 758, 1616, 2042
BspT107I GGYRCC 1 cut(s) 2421
BspTI CTTAAG 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 518
BsrBI CCGCTC 3 cut(s) 699, 915, 1257
BsrDI GCAATG 2 cut(s) 1722, 2004
BsrFI RCCGGY 1 cut(s) 282
BsrI ACTGG 8 cut(s) 13, 648, 1200, 1283, 1637, 1781, 2028, 2201
BssAI RCCGGY 1 cut(s) 282
BssECI CCNNGG 5 cut(s) 238, 1227, 1933, 2167, 2364
BssMI GATC 6 cut(s) 348, 477, 676, 1362, 2372, 2376
BssT1I CCWWGG 1 cut(s) 2364
Bst2UI CCWGG 6 cut(s) 97, 111, 570, 1171, 1228, 2168
Bst4CI ACNGT 4 cut(s) 742, 1052, 1601, 1744
Bst6I CTCTTC 1 cut(s) 443
BstAFI CTTAAG 1 cut(s) 224
BstAPI GCANNNNNTGC 1 cut(s) 1929
BstBI TTCGAA 4 cut(s) 317, 758, 1616, 2042
BstC8I GCNNGC 8 cut(s) 65, 284, 412, 1147, 1151, 1155, 1255, 2403
BstF5I GGATG 4 cut(s) 216, 414, 465, 1987
BstFNI CGCG 3 cut(s) 1589, 1791, 1949
BstHHI GCGC 2 cut(s) 1628, 1791
BstKTI GATC 6 cut(s) 351, 480, 679, 1365, 2375, 2379
BstMAI GTCTC 5 cut(s) 225, 518, 901, 1681, 1764
BstMBI GATC 6 cut(s) 348, 477, 676, 1362, 2372, 2376
BstMCI CGRYCG 1 cut(s) 283
BstMWI GCNNNNNNNGC 8 cut(s) 292, 653, 1003, 1453, 1929, 2225, 2333, 2411
BstNI CCWGG 6 cut(s) 97, 111, 570, 1171, 1228, 2168
BstNSI RCATGY 2 cut(s) 838, 2251
BstUI CGCG 3 cut(s) 1589, 1791, 1949
BstV1I GCAGC 5 cut(s) 391, 1308, 1442, 2051, 2215
BstV2I GAAGAC 3 cut(s) 1533, 2150, 2158
BstXI CCANNNNNNTGG 2 cut(s) 1153, 1978
BstZI CGGCCG 1 cut(s) 280
Bsu15I ATCGAT 2 cut(s) 351, 2375
BsuI GTATCC 1 cut(s) 1867
BsuTUI ATCGAT 2 cut(s) 351, 2375
BtgZI GCGATG 1 cut(s) 821
BtsCI GGATG 4 cut(s) 216, 414, 465, 1987
BtsI GCAGTG 1 cut(s) 946
BtsIMutI CAGTG 4 cut(s) 6, 641, 946, 1207
BveI ACCTGC 1 cut(s) 1461
Cac8I GCNNGC 8 cut(s) 65, 284, 412, 1147, 1151, 1155, 1255, 2403
CfoI GCGC 2 cut(s) 1628, 1791
Cfr10I RCCGGY 1 cut(s) 282
Cfr13I GGNCC 5 cut(s) 185, 235, 846, 1083, 1417
Cfr9I CCCGGG 1 cut(s) 1933
ClaI ATCGAT 2 cut(s) 351, 2375
CseI GACGC 1 cut(s) 1039
Csp6I GTAC 3 cut(s) 229, 2098, 2104
CviQI GTAC 3 cut(s) 229, 2098, 2104
DpnI GATC 6 cut(s) 350, 479, 678, 1364, 2374, 2378
DpnII GATC 6 cut(s) 348, 477, 676, 1362, 2372, 2376
EaeI YGGCCR 4 cut(s) 273, 280, 408, 2198
EagI CGGCCG 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
EciI GGCGGA 2 cut(s) 330, 2256
Ecl136II GAGCTC 2 cut(s) 67, 85
EclXI CGGCCG 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 2364
Eco147I AGGCCT 3 cut(s) 795, 867, 2166
Eco24I GRGCYC 3 cut(s) 15, 69, 87
Eco31I GGTCTC 1 cut(s) 518
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 3 cut(s) 235, 846, 1083
Eco52I CGGCCG 1 cut(s) 280
Eco53kI GAGCTC 2 cut(s) 67, 85
Eco57I CTGAAG 2 cut(s) 467, 1055
Eco88I CYCGRG 4 cut(s) 517, 524, 1399, 1933
EcoICRI GAGCTC 2 cut(s) 67, 85
EcoO109I RGGNCCY 1 cut(s) 846
EcoRI GAATTC 2 cut(s) 2000, 2318
EcoRII CCWGG 6 cut(s) 95, 109, 568, 1169, 1226, 2166
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 2364
EcoT38I GRGCYC 3 cut(s) 15, 69, 87
ErhI CCWWGG 1 cut(s) 2364
Esp3I CGTCTC 1 cut(s) 225
FaqI GGGAC 3 cut(s) 859, 1703, 2041
FauI CCCGC 1 cut(s) 1150
FblI GTMKAC 2 cut(s) 683, 1041
FokI GGATG 4 cut(s) 223, 401, 472, 1994
FriOI GRGCYC 3 cut(s) 15, 69, 87
FspBI CTAG 4 cut(s) 77, 1296, 1715, 2348
GlaI GCGC 2 cut(s) 1627, 1790
GsaI CCCAGC 1 cut(s) 1502
GsuI CTGGAG 1 cut(s) 1798
HgaI GACGC 1 cut(s) 1039
HhaI GCGC 2 cut(s) 1628, 1791
Hin6I GCGC 2 cut(s) 1626, 1789
HinP1I GCGC 2 cut(s) 1626, 1789
HindIII AAGCTT 4 cut(s) 308, 885, 1550, 2087
HphI GGTGA 4 cut(s) 1073, 1810, 1949, 1963
Hpy166II GTNNAC 4 cut(s) 31, 684, 1042, 2098
Hpy8I GTNNAC 4 cut(s) 31, 684, 1042, 2098
Hpy99I CGWCG 1 cut(s) 2206
HpyAV CCTTC 6 cut(s) 551, 805, 878, 1030, 1658, 2418
HpyCH4III ACNGT 4 cut(s) 742, 1052, 1601, 1744
HpyCH4IV ACGT 2 cut(s) 1234, 1854
HpyCH4V TGCA 8 cut(s) 488, 939, 955, 2067, 2212, 2231, 2251, 2401
HpyF10VI GCNNNNNNNGC 8 cut(s) 292, 653, 1003, 1453, 1929, 2225, 2333, 2411
HpySE526I ACGT 2 cut(s) 1234, 1854
HspAI GCGC 2 cut(s) 1626, 1789
Kpn2I TCCGGA 2 cut(s) 496, 781
KroI GCCGGC 1 cut(s) 282
KroNI GCCGGC 1 cut(s) 284
Kzo9I GATC 6 cut(s) 348, 477, 676, 1362, 2372, 2376
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 4 cut(s) 72, 90, 292, 2410
Lsp1109I GCAGC 5 cut(s) 391, 1308, 1442, 2051, 2215
LweI GCATC 4 cut(s) 372, 423, 1098, 2168
MaeI CTAG 4 cut(s) 77, 1296, 1715, 2348
MaeII ACGT 2 cut(s) 1234, 1854
MaeIII GTNAC 8 cut(s) 4, 606, 1115, 1658, 1738, 1964, 2075, 2243
MalI GATC 6 cut(s) 350, 479, 678, 1364, 2374, 2378
MbiI CCGCTC 3 cut(s) 699, 915, 1257
MboI GATC 6 cut(s) 348, 477, 676, 1362, 2372, 2376
MboII GAAGA 8 cut(s) 451, 460, 574, 601, 736, 1533, 2155, 2163
MhlI GDGCHC 3 cut(s) 15, 69, 87
MmeI TCCRAC 2 cut(s) 1569, 2289
MroI TCCGGA 2 cut(s) 496, 781
MroNI GCCGGC 1 cut(s) 282
MroXI GAANNNNTTC 3 cut(s) 323, 1481, 2000
MseI TTAA 7 cut(s) 225, 615, 767, 1248, 1533, 1644, 2052
MslI CAYNNNNRTG 2 cut(s) 1727, 2283
MspA1I CMGCKG 3 cut(s) 910, 1309, 1502
MspCI CTTAAG 1 cut(s) 224
MvaI CCWGG 6 cut(s) 97, 111, 570, 1171, 1228, 2168
MvnI CGCG 3 cut(s) 1589, 1791, 1949
MwoI GCNNNNNNNGC 8 cut(s) 292, 653, 1003, 1453, 1929, 2225, 2333, 2411
NaeI GCCGGC 1 cut(s) 284
NciI CCSGG 5 cut(s) 50, 901, 1653, 1934, 1935
NdeII GATC 6 cut(s) 348, 477, 676, 1362, 2372, 2376
NgoMIV GCCGGC 1 cut(s) 282
NlaIV GGNNCC 4 cut(s) 847, 1068, 2406, 2423
NmuCI GTSAC 3 cut(s) 4, 1115, 2075
NspI RCATGY 2 cut(s) 838, 2251
NspV TTCGAA 4 cut(s) 317, 758, 1616, 2042
PaeR7I CTCGAG 3 cut(s) 517, 524, 1399
PceI AGGCCT 3 cut(s) 795, 867, 2166
PciI ACATGT 1 cut(s) 834
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 2 cut(s) 375, 1406
PdiI GCCGGC 1 cut(s) 284
PdmI GAANNNNTTC 3 cut(s) 323, 1481, 2000
PfoI TCCNGGA 2 cut(s) 48, 899
PpuMI RGGWCCY 1 cut(s) 846
PscI ACATGT 1 cut(s) 834
PsiI TTATAA 1 cut(s) 390
Psp124BI GAGCTC 2 cut(s) 69, 87
Psp1406I AACGTT 1 cut(s) 1234
Psp5II RGGWCCY 1 cut(s) 846
Psp6I CCWGG 6 cut(s) 95, 109, 568, 1169, 1226, 2166
PspFI CCCAGC 1 cut(s) 1498
PspGI CCWGG 6 cut(s) 95, 109, 568, 1169, 1226, 2166
PspN4I GGNNCC 4 cut(s) 847, 1068, 2406, 2423
PspPI GGNCC 5 cut(s) 185, 235, 846, 1083, 1417
PspPPI RGGWCCY 1 cut(s) 846
PvuII CAGCTG 3 cut(s) 910, 1309, 1502
RsaI GTAC 3 cut(s) 230, 2099, 2105
RsaNI GTAC 3 cut(s) 229, 2098, 2104
RseI CAYNNNNRTG 2 cut(s) 1727, 2283
SacI GAGCTC 2 cut(s) 69, 87
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 7 cut(s) 225, 615, 767, 1248, 1533, 1644, 2052
Sau3AI GATC 6 cut(s) 348, 477, 676, 1362, 2372, 2376
Sau96I GGNCC 5 cut(s) 185, 235, 846, 1083, 1417
ScaI AGTACT 1 cut(s) 230
SduI GDGCHC 3 cut(s) 15, 69, 87
SfaNI GCATC 4 cut(s) 372, 423, 1098, 2168
Sfr274I CTCGAG 3 cut(s) 517, 524, 1399
SfuI TTCGAA 4 cut(s) 317, 758, 1616, 2042
SinI GGWCC 3 cut(s) 235, 846, 1083
SlaI CTCGAG 3 cut(s) 517, 524, 1399
SmaI CCCGGG 1 cut(s) 1935
SmiMI CAYNNNNRTG 2 cut(s) 1727, 2283
SmlI CTYRAG 6 cut(s) 224, 305, 517, 524, 1399, 1991
SmoI CTYRAG 6 cut(s) 224, 305, 517, 524, 1399, 1991
SpeI ACTAGT 1 cut(s) 1295
SseBI AGGCCT 3 cut(s) 795, 867, 2166
SspI AATATT 1 cut(s) 375
SspMI CTAG 4 cut(s) 77, 1296, 1715, 2348
SstI GAGCTC 2 cut(s) 69, 87
StuI AGGCCT 3 cut(s) 795, 867, 2166
StyI CCWWGG 1 cut(s) 2364
TaaI ACNGT 4 cut(s) 742, 1052, 1601, 1744
TaiI ACGT 2 cut(s) 1237, 1857
TaqII GACCGA 1 cut(s) 470
TatI WGTACW 2 cut(s) 228, 2103
TauI GCSGC 5 cut(s) 275, 410, 702, 856, 918
Tru1I TTAA 7 cut(s) 225, 615, 767, 1248, 1533, 1644, 2052
Tru9I TTAA 7 cut(s) 225, 615, 767, 1248, 1533, 1644, 2052
TscAI CASTG 4 cut(s) 13, 648, 946, 1207
TseFI GTSAC 3 cut(s) 4, 1115, 2075
TseI GCWGC 5 cut(s) 404, 1321, 1430, 2064, 2228
Tsp45I GTSAC 3 cut(s) 4, 1115, 2075
TspDTI ATGAA 4 cut(s) 824, 1608, 1993, 2267
TspGWI ACGGA 8 cut(s) 53, 305, 408, 437, 479, 1661, 1886, 2385
TspMI CCCGGG 1 cut(s) 1933
TspRI CASTG 4 cut(s) 13, 648, 946, 1207
Vha464I CTTAAG 1 cut(s) 224
VpaK11BI GGWCC 3 cut(s) 235, 846, 1083
XapI RAATTY 6 cut(s) 125, 433, 623, 661, 2000, 2318
XceI RCATGY 2 cut(s) 838, 2251
XhoI CTCGAG 3 cut(s) 517, 524, 1399
XmaI CCCGGG 1 cut(s) 1933
XmiI GTMKAC 2 cut(s) 683, 1041
XmnI GAANNNNTTC 3 cut(s) 323, 1481, 2000
XspI CTAG 4 cut(s) 77, 1296, 1715, 2348
ZrmI AGTACT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.