Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1861 |
| AccB1I | GGYRCC | 2 cut(s) | 292, 1324 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1100 |
| AccBSI | CCGCTC | 1 cut(s) | 810 |
| AccI | GTMKAC | 2 cut(s) | 378, 2331 |
| AccII | CGCG | 6 cut(s) | 1405, 1532, 2151, 2420, 2514, 2785 |
| AccIII | TCCGGA | 1 cut(s) | 1732 |
| AclWI | GGATC | 5 cut(s) | 178, 191, 1743, 1792, 2367 |
| AcoI | YGGCCR | 1 cut(s) | 2580 |
| AcsI | RAATTY | 7 cut(s) | 228, 715, 829, 1728, 2071, 2088, 2209 |
| AcuI | CTGAAG | 1 cut(s) | 533 |
| AcyI | GRCGYC | 1 cut(s) | 1325 |
| AfaI | GTAC | 5 cut(s) | 401, 557, 1962, 2339, 2970 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 521, 522, 525, 593, 1100, 1591 |
| AflIII | ACRYGT | 2 cut(s) | 624, 2694 |
| AgeI | ACCGGT | 1 cut(s) | 289 |
| AhlI | ACTAGT | 1 cut(s) | 1619 |
| AjiI | CACGTC | 2 cut(s) | 2518, 2695 |
| AjnI | CCWGG | 3 cut(s) | 218, 1460, 2355 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1321, 1353 |
| AleI | CACNNNNGTG | 1 cut(s) | 2490 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 114, 146, 647, 679 |
| Alw21I | GWGCWC | 3 cut(s) | 540, 2264, 2870 |
| Alw26I | GTCTC | 5 cut(s) | 417, 1413, 2306, 2562, 2896 |
| AlwI | GGATC | 5 cut(s) | 178, 191, 1743, 1792, 2367 |
| Ama87I | CYCGRG | 4 cut(s) | 539, 879, 1742, 2398 |
| Aor13HI | TCCGGA | 1 cut(s) | 1732 |
| ApeKI | GCWGC | 6 cut(s) | 1254, 1820, 2298, 2368, 2459, 2958 |
| ApoI | RAATTY | 7 cut(s) | 228, 715, 829, 1728, 2071, 2088, 2209 |
| AsiGI | ACCGGT | 1 cut(s) | 289 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 566 |
| AspLEI | GCGC | 8 cut(s) | 1327, 1534, 1862, 1942, 2422, 2459, 2785, 3027 |
| AspS9I | GGNCC | 3 cut(s) | 406, 431, 2467 |
| AsuC2I | CCSGG | 3 cut(s) | 357, 463, 1683 |
| AsuHPI | GGTGA | 7 cut(s) | 244, 515, 1567, 1639, 1775, 2183, 3073 |
| AvaI | CYCGRG | 4 cut(s) | 539, 879, 1742, 2398 |
| AvaII | GGWCC | 1 cut(s) | 406 |
| AxyI | CCTNAGG | 1 cut(s) | 1518 |
| BalI | TGGCCA | 1 cut(s) | 2582 |
| BamHI | GGATCC | 1 cut(s) | 183 |
| BanI | GGYRCC | 2 cut(s) | 292, 1324 |
| BanII | GRGCYC | 2 cut(s) | 540, 2264 |
| BbsI | GAAGAC | 5 cut(s) | 1271, 1449, 1667, 3038, 3048 |
| Bbv12I | GWGCWC | 3 cut(s) | 540, 2264, 2870 |
| BbvCI | CCTCAGC | 1 cut(s) | 2471 |
| BbvI | GCAGC | 6 cut(s) | 1266, 1807, 2310, 2355, 2446, 2970 |
| BccI | CCATC | 9 cut(s) | 304, 745, 1468, 1597, 1994, 2358, 2395, 2418, 3044 |
| BceAI | ACGGC | 5 cut(s) | 722, 1338, 1501, 2767, 2946 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1851, 1885, 2978, 3012 |
| BciT130I | CCWGG | 3 cut(s) | 220, 1462, 2357 |
| BciVI | GTATCC | 1 cut(s) | 696 |
| BclI | TGATCA | 1 cut(s) | 1561 |
| BcnI | CCSGG | 3 cut(s) | 357, 463, 1683 |
| BcoDI | GTCTC | 5 cut(s) | 417, 1413, 2306, 2562, 2896 |
| BcuI | ACTAGT | 1 cut(s) | 1619 |
| BfaI | CTAG | 5 cut(s) | 395, 1308, 1539, 1620, 1866 |
| BfoI | RGCGCY | 2 cut(s) | 1328, 2460 |
| BfuI | GTATCC | 1 cut(s) | 696 |
| BisI | GCNGC | 7 cut(s) | 1255, 1821, 2299, 2369, 2460, 2498, 2959 |
| BlsI | GCNGC | 7 cut(s) | 1256, 1822, 2300, 2370, 2461, 2499, 2960 |
| BmcAI | AGTACT | 3 cut(s) | 401, 2339, 2970 |
| Bme1390I | CCNGG | 6 cut(s) | 220, 357, 463, 1462, 1683, 2357 |
| Bme18I | GGWCC | 1 cut(s) | 406 |
| BmeT110I | CYCGRG | 4 cut(s) | 539, 879, 1742, 2398 |
| BmgBI | CACGTC | 2 cut(s) | 2518, 2695 |
| BmgT120I | GGNCC | 3 cut(s) | 406, 431, 2467 |
| BmiI | GGNNCC | 7 cut(s) | 185, 294, 433, 876, 1326, 2354, 2635 |
| BmrFI | CCNGG | 6 cut(s) | 220, 357, 463, 1462, 1683, 2357 |
| BmrI | ACTGGG | 1 cut(s) | 1679 |
| BmsI | GCATC | 4 cut(s) | 1756, 2518, 2809, 2945 |
| BmuI | ACTGGG | 1 cut(s) | 1679 |
| BoxI | GACNNNNGTC | 2 cut(s) | 2574, 3068 |
| BpiI | GAAGAC | 5 cut(s) | 1271, 1449, 1667, 3038, 3048 |
| BpmI | CTGGAG | 4 cut(s) | 1077, 1483, 2637, 2741 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2463, 2471 |
| BpuEI | CTTGAG | 5 cut(s) | 128, 674, 922, 2246, 2278 |
| BpuMI | CCSGG | 3 cut(s) | 357, 463, 1683 |
| Bsa29I | ATCGAT | 1 cut(s) | 2362 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1566 |
| BsaHI | GRCGYC | 1 cut(s) | 1325 |
| BsaJI | CCNNGG | 5 cut(s) | 355, 461, 650, 2502, 3036 |
| BsaWI | WCCGGW | 5 cut(s) | 289, 500, 1151, 1649, 1732 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 482, 512 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 521, 522, 525, 593, 1100, 1591 |
| Bse118I | RCCGGY | 4 cut(s) | 289, 1367, 1577, 2732 |
| Bse1I | ACTGG | 8 cut(s) | 565, 774, 1674, 2018, 2307, 2405, 2432, 2966 |
| Bse21I | CCTNAGG | 1 cut(s) | 1518 |
| Bse3DI | GCAATG | 3 cut(s) | 1026, 2173, 2646 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1566 |
| BseAI | TCCGGA | 1 cut(s) | 1732 |
| BseBI | CCWGG | 3 cut(s) | 220, 1462, 2357 |
| BseCI | ATCGAT | 1 cut(s) | 2362 |
| BseDI | CCNNGG | 5 cut(s) | 355, 461, 650, 2502, 3036 |
| BseGI | GGATG | 3 cut(s) | 296, 2387, 2712 |
| BseJI | GATNNNNATC | 1 cut(s) | 1566 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 521, 522, 525, 593, 1100, 1591 |
| BseMI | GCAATG | 3 cut(s) | 1026, 2173, 2646 |
| BseNI | ACTGG | 8 cut(s) | 565, 774, 1674, 2018, 2307, 2405, 2432, 2966 |
| BseRI | GAGGAG | 9 cut(s) | 44, 47, 50, 62, 65, 68, 71, 74, 2994 |
| BseXI | GCAGC | 6 cut(s) | 1266, 1807, 2310, 2355, 2446, 2970 |
| BsgI | GTGCAG | 3 cut(s) | 220, 2317, 2354 |
| Bsh1236I | CGCG | 6 cut(s) | 1405, 1532, 2151, 2420, 2514, 2785 |
| BshNI | GGYRCC | 2 cut(s) | 292, 1324 |
| BshTI | ACCGGT | 1 cut(s) | 289 |
| BshVI | ATCGAT | 1 cut(s) | 2362 |
| BsiHKAI | GWGCWC | 3 cut(s) | 540, 2264, 2870 |
| BsiHKCI | CYCGRG | 4 cut(s) | 539, 879, 1742, 2398 |
| BslFI | GGGAC | 4 cut(s) | 566, 751, 952, 1698 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 521, 522, 525, 593, 1100, 1591 |
| BsmAI | GTCTC | 5 cut(s) | 417, 1413, 2306, 2562, 2896 |
| BsmFI | GGGAC | 4 cut(s) | 566, 751, 952, 1698 |
| BsmI | GAATGC | 2 cut(s) | 362, 906 |
| BsoBI | CYCGRG | 4 cut(s) | 539, 879, 1742, 2398 |
| Bsp1286I | GDGCHC | 3 cut(s) | 540, 2264, 2870 |
| Bsp13I | TCCGGA | 1 cut(s) | 1732 |
| Bsp1407I | TGTACA | 1 cut(s) | 555 |
| Bsp68I | TCGCGA | 1 cut(s) | 2514 |
| BspDI | ATCGAT | 1 cut(s) | 2362 |
| BspEI | TCCGGA | 1 cut(s) | 1732 |
| BspFNI | CGCG | 6 cut(s) | 1405, 1532, 2151, 2420, 2514, 2785 |
| BspHI | TCATGA | 2 cut(s) | 852, 1279 |
| BspLI | GGNNCC | 7 cut(s) | 185, 294, 433, 876, 1326, 2354, 2635 |
| BspPI | GGATC | 5 cut(s) | 178, 191, 1743, 1792, 2367 |
| BspQI | GCTCTTC | 1 cut(s) | 1016 |
| BspT107I | GGYRCC | 2 cut(s) | 292, 1324 |
| BsrBI | CCGCTC | 1 cut(s) | 810 |
| BsrDI | GCAATG | 3 cut(s) | 1026, 2173, 2646 |
| BsrFI | RCCGGY | 4 cut(s) | 289, 1367, 1577, 2732 |
| BsrGI | TGTACA | 1 cut(s) | 555 |
| BsrI | ACTGG | 8 cut(s) | 565, 774, 1674, 2018, 2307, 2405, 2432, 2966 |
| BssAI | RCCGGY | 4 cut(s) | 289, 1367, 1577, 2732 |
| BssECI | CCNNGG | 5 cut(s) | 355, 461, 650, 2502, 3036 |
| BssNI | GRCGYC | 1 cut(s) | 1325 |
| BssT1I | CCWWGG | 2 cut(s) | 650, 3036 |
| Bst2UI | CCWGG | 3 cut(s) | 220, 1462, 2357 |
| Bst4CI | ACNGT | 7 cut(s) | 328, 788, 1270, 1293, 1965, 2027, 2492 |
| Bst6I | CTCTTC | 6 cut(s) | 854, 945, 1016, 1780, 2012, 2211 |
| BstACI | GRCGYC | 1 cut(s) | 1325 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2947 |
| BstAUI | TGTACA | 1 cut(s) | 555 |
| BstC8I | GCNNGC | 5 cut(s) | 458, 1033, 1037, 1373, 2632 |
| BstDSI | CCRYGG | 1 cut(s) | 2502 |
| BstEII | GGTNACC | 1 cut(s) | 3061 |
| BstF5I | GGATG | 3 cut(s) | 296, 2387, 2712 |
| BstFNI | CGCG | 6 cut(s) | 1405, 1532, 2151, 2420, 2514, 2785 |
| BstH2I | RGCGCY | 2 cut(s) | 1328, 2460 |
| BstHHI | GCGC | 8 cut(s) | 1327, 1534, 1862, 1942, 2422, 2459, 2785, 3027 |
| BstMAI | GTCTC | 5 cut(s) | 417, 1413, 2306, 2562, 2896 |
| BstNI | CCWGG | 3 cut(s) | 220, 1462, 2357 |
| BstNSI | RCATGY | 3 cut(s) | 628, 2276, 2996 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 2574, 3068 |
| BstPI | GGTNACC | 1 cut(s) | 3061 |
| BstSCI | CCNGG | 6 cut(s) | 218, 355, 461, 1460, 1681, 2355 |
| BstUI | CGCG | 6 cut(s) | 1405, 1532, 2151, 2420, 2514, 2785 |
| BstV1I | GCAGC | 6 cut(s) | 1266, 1807, 2310, 2355, 2446, 2970 |
| BstV2I | GAAGAC | 5 cut(s) | 1271, 1449, 1667, 3038, 3048 |
| BstX2I | RGATCY | 2 cut(s) | 183, 1797 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 572, 1721, 2590 |
| BstYI | RGATCY | 2 cut(s) | 183, 1797 |
| Bsu15I | ATCGAT | 1 cut(s) | 2362 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1518 |
| BsuI | GTATCC | 1 cut(s) | 696 |
| BsuTUI | ATCGAT | 1 cut(s) | 2362 |
| BtgI | CCRYGG | 1 cut(s) | 2502 |
| BtgZI | GCGATG | 2 cut(s) | 2495, 2769 |
| BtrI | CACGTC | 2 cut(s) | 2518, 2695 |
| BtsCI | GGATG | 3 cut(s) | 296, 2387, 2712 |
| BtsI | GCAGTG | 3 cut(s) | 211, 2457, 2763 |
| BtsIMutI | CAGTG | 6 cut(s) | 211, 1228, 2314, 2457, 2763, 2895 |
| BtuMI | TCGCGA | 1 cut(s) | 2514 |
| Cac8I | GCNNGC | 5 cut(s) | 458, 1033, 1037, 1373, 2632 |
| CciI | TCATGA | 2 cut(s) | 852, 1279 |
| CfoI | GCGC | 8 cut(s) | 1327, 1534, 1862, 1942, 2422, 2459, 2785, 3027 |
| Cfr10I | RCCGGY | 4 cut(s) | 289, 1367, 1577, 2732 |
| Cfr13I | GGNCC | 3 cut(s) | 406, 431, 2467 |
| ClaI | ATCGAT | 1 cut(s) | 2362 |
| CseI | GACGC | 1 cut(s) | 2070 |
| Csp6I | GTAC | 5 cut(s) | 400, 556, 1961, 2338, 2969 |
| CspAI | ACCGGT | 1 cut(s) | 289 |
| CviQI | GTAC | 5 cut(s) | 400, 556, 1961, 2338, 2969 |
| DinI | GGCGCC | 1 cut(s) | 1326 |
| EaeI | YGGCCR | 1 cut(s) | 2580 |
| Eam1104I | CTCTTC | 6 cut(s) | 854, 945, 1016, 1780, 2012, 2211 |
| EarI | CTCTTC | 6 cut(s) | 854, 945, 1016, 1780, 2012, 2211 |
| EciI | GGCGGA | 1 cut(s) | 153 |
| Ecl136II | GAGCTC | 2 cut(s) | 538, 2262 |
| Eco130I | CCWWGG | 2 cut(s) | 650, 3036 |
| Eco147I | AGGCCT | 2 cut(s) | 1854, 2920 |
| Eco24I | GRGCYC | 2 cut(s) | 540, 2264 |
| Eco47I | GGWCC | 1 cut(s) | 406 |
| Eco53kI | GAGCTC | 2 cut(s) | 538, 2262 |
| Eco57I | CTGAAG | 1 cut(s) | 533 |
| Eco81I | CCTNAGG | 1 cut(s) | 1518 |
| Eco88I | CYCGRG | 4 cut(s) | 539, 879, 1742, 2398 |
| Eco91I | GGTNACC | 1 cut(s) | 3061 |
| EcoICRI | GAGCTC | 2 cut(s) | 538, 2262 |
| EcoO109I | RGGNCCY | 2 cut(s) | 431, 2467 |
| EcoO65I | GGTNACC | 1 cut(s) | 3061 |
| EcoRII | CCWGG | 3 cut(s) | 218, 1460, 2355 |
| EcoT14I | CCWWGG | 2 cut(s) | 650, 3036 |
| EcoT38I | GRGCYC | 2 cut(s) | 540, 2264 |
| EgeI | GGCGCC | 1 cut(s) | 1326 |
| EheI | GGCGCC | 1 cut(s) | 1326 |
| ErhI | CCWWGG | 2 cut(s) | 650, 3036 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 137, 169, 2208, 2240, 2592, 2624, 3020, 3052 |
| FaqI | GGGAC | 4 cut(s) | 566, 751, 952, 1698 |
| FauI | CCCGC | 3 cut(s) | 523, 1040, 2123 |
| FbaI | TGATCA | 1 cut(s) | 1561 |
| FblI | GTMKAC | 2 cut(s) | 378, 2331 |
| Fnu4HI | GCNGC | 7 cut(s) | 1255, 1821, 2299, 2369, 2460, 2498, 2959 |
| FokI | GGATG | 3 cut(s) | 283, 2374, 2699 |
| FriOI | GRGCYC | 2 cut(s) | 540, 2264 |
| Fsp4HI | GCNGC | 7 cut(s) | 1255, 1821, 2299, 2369, 2460, 2498, 2959 |
| FspBI | CTAG | 5 cut(s) | 395, 1308, 1539, 1620, 1866 |
| FspI | TGCGCA | 1 cut(s) | 1861 |
| GlaI | GCGC | 8 cut(s) | 1326, 1533, 1861, 1941, 2421, 2458, 2784, 3026 |
| GluI | GCNGC | 7 cut(s) | 1255, 1821, 2299, 2369, 2460, 2498, 2959 |
| GsuI | CTGGAG | 4 cut(s) | 1077, 1483, 2637, 2741 |
| HaeII | RGCGCY | 2 cut(s) | 1328, 2460 |
| HgaI | GACGC | 1 cut(s) | 2070 |
| HhaI | GCGC | 8 cut(s) | 1327, 1534, 1862, 1942, 2422, 2459, 2785, 3027 |
| Hin1I | GRCGYC | 1 cut(s) | 1325 |
| Hin6I | GCGC | 8 cut(s) | 1325, 1532, 1860, 1940, 2420, 2457, 2783, 3025 |
| HinP1I | GCGC | 8 cut(s) | 1325, 1532, 1860, 1940, 2420, 2457, 2783, 3025 |
| HincII | GTYRAC | 1 cut(s) | 2727 |
| HindII | GTYRAC | 1 cut(s) | 2727 |
| HindIII | AAGCTT | 3 cut(s) | 1385, 1834, 2222 |
| HphI | GGTGA | 7 cut(s) | 244, 515, 1567, 1639, 1775, 2183, 3073 |
| Hpy166II | GTNNAC | 4 cut(s) | 379, 1320, 2332, 2727 |
| Hpy8I | GTNNAC | 4 cut(s) | 379, 1320, 2332, 2727 |
| Hpy99I | CGWCG | 2 cut(s) | 2519, 2696 |
| HpyAV | CCTTC | 9 cut(s) | 74, 310, 672, 865, 1595, 1823, 2726, 2923, 2931 |
| HpyCH4III | ACNGT | 7 cut(s) | 328, 788, 1270, 1293, 1965, 2027, 2492 |
| HpyCH4IV | ACGT | 2 cut(s) | 2517, 2694 |
| HpySE526I | ACGT | 2 cut(s) | 2517, 2694 |
| Hsp92I | GRCGYC | 1 cut(s) | 1325 |
| HspAI | GCGC | 8 cut(s) | 1325, 1532, 1860, 1940, 2420, 2457, 2783, 3025 |
| KasI | GGCGCC | 1 cut(s) | 1324 |
| Kpn2I | TCCGGA | 1 cut(s) | 1732 |
| Ksp22I | TGATCA | 1 cut(s) | 1561 |
| LguI | GCTCTTC | 1 cut(s) | 1016 |
| LmnI | GCTCC | 9 cut(s) | 221, 359, 1887, 2352, 2639, 2656, 2873, 2955, 3015 |
| Lsp1109I | GCAGC | 6 cut(s) | 1266, 1807, 2310, 2355, 2446, 2970 |
| LweI | GCATC | 4 cut(s) | 1756, 2518, 2809, 2945 |
| MaeI | CTAG | 5 cut(s) | 395, 1308, 1539, 1620, 1866 |
| MaeII | ACGT | 2 cut(s) | 2517, 2694 |
| MaeIII | GTNAC | 8 cut(s) | 250, 596, 1611, 2009, 2593, 2789, 2849, 3061 |
| MbiI | CCGCTC | 1 cut(s) | 810 |
| MflI | RGATCY | 2 cut(s) | 183, 1797 |
| MhlI | GDGCHC | 3 cut(s) | 540, 2264, 2870 |
| MlsI | TGGCCA | 1 cut(s) | 2582 |
| MluNI | TGGCCA | 1 cut(s) | 2582 |
| Mly113I | GGCGCC | 1 cut(s) | 1325 |
| MlyI | GAGTC | 1 cut(s) | 1619 |
| MmeI | TCCRAC | 2 cut(s) | 1389, 2520 |
| Mox20I | TGGCCA | 1 cut(s) | 2582 |
| MroI | TCCGGA | 1 cut(s) | 1732 |
| MroXI | GAANNNNTTC | 1 cut(s) | 566 |
| MscI | TGGCCA | 1 cut(s) | 2582 |
| MseI | TTAA | 5 cut(s) | 155, 932, 974, 1526, 1626 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1221, 2490 |
| Msp20I | TGGCCA | 1 cut(s) | 2582 |
| MspA1I | CMGCKG | 1 cut(s) | 553 |
| MspR9I | CCNGG | 6 cut(s) | 220, 357, 463, 1462, 1683, 2357 |
| Mva1269I | GAATGC | 2 cut(s) | 362, 906 |
| MvaI | CCWGG | 3 cut(s) | 220, 1462, 2357 |
| MvnI | CGCG | 6 cut(s) | 1405, 1532, 2151, 2420, 2514, 2785 |
| NarI | GGCGCC | 1 cut(s) | 1325 |
| NciI | CCSGG | 3 cut(s) | 357, 463, 1683 |
| NlaIV | GGNNCC | 7 cut(s) | 185, 294, 433, 876, 1326, 2354, 2635 |
| NmeAIII | GCCGAG | 1 cut(s) | 2862 |
| NmuCI | GTSAC | 6 cut(s) | 250, 1611, 2593, 2789, 2849, 3061 |
| NruI | TCGCGA | 1 cut(s) | 2514 |
| NsbI | TGCGCA | 1 cut(s) | 1861 |
| NspI | RCATGY | 3 cut(s) | 628, 2276, 2996 |
| OliI | CACNNNNGTG | 1 cut(s) | 2490 |
| PaeR7I | CTCGAG | 3 cut(s) | 539, 879, 2398 |
| PagI | TCATGA | 2 cut(s) | 852, 1279 |
| PceI | AGGCCT | 2 cut(s) | 1854, 2920 |
| PciI | ACATGT | 1 cut(s) | 624 |
| PciSI | GCTCTTC | 1 cut(s) | 1016 |
| PctI | GAATGC | 2 cut(s) | 362, 906 |
| PdmI | GAANNNNTTC | 1 cut(s) | 566 |
| PfeI | GAWTC | 9 cut(s) | 544, 802, 890, 1213, 1567, 1901, 2036, 2047, 2543 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1100 |
| PfoI | TCCNGGA | 1 cut(s) | 1460 |
| PinAI | ACCGGT | 1 cut(s) | 289 |
| PkrI | GCNGC | 7 cut(s) | 1256, 1822, 2300, 2370, 2461, 2499, 2960 |
| PleI | GAGTC | 1 cut(s) | 1618 |
| PluTI | GGCGCC | 1 cut(s) | 1328 |
| PpsI | GAGTC | 1 cut(s) | 1618 |
| PscI | ACATGT | 1 cut(s) | 624 |
| PshAI | GACNNNNGTC | 2 cut(s) | 2574, 3068 |
| Psp124BI | GAGCTC | 2 cut(s) | 540, 2264 |
| Psp6I | CCWGG | 3 cut(s) | 218, 1460, 2355 |
| PspEI | GGTNACC | 1 cut(s) | 3061 |
| PspGI | CCWGG | 3 cut(s) | 218, 1460, 2355 |
| PspN4I | GGNNCC | 7 cut(s) | 185, 294, 433, 876, 1326, 2354, 2635 |
| PspPI | GGNCC | 3 cut(s) | 406, 431, 2467 |
| PspXI | VCTCGAGB | 1 cut(s) | 879 |
| PsuI | RGATCY | 2 cut(s) | 183, 1797 |
| PvuII | CAGCTG | 1 cut(s) | 553 |
| RruI | TCGCGA | 1 cut(s) | 2514 |
| RsaI | GTAC | 5 cut(s) | 401, 557, 1962, 2339, 2970 |
| RsaNI | GTAC | 5 cut(s) | 400, 556, 1961, 2338, 2969 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1221, 2490 |
| SacI | GAGCTC | 2 cut(s) | 540, 2264 |
| SapI | GCTCTTC | 1 cut(s) | 1016 |
| SaqAI | TTAA | 5 cut(s) | 155, 932, 974, 1526, 1626 |
| SatI | GCNGC | 7 cut(s) | 1255, 1821, 2299, 2369, 2460, 2498, 2959 |
| Sau96I | GGNCC | 3 cut(s) | 406, 431, 2467 |
| ScaI | AGTACT | 3 cut(s) | 401, 2339, 2970 |
| SchI | GAGTC | 1 cut(s) | 1619 |
| ScrFI | CCNGG | 6 cut(s) | 220, 357, 463, 1462, 1683, 2357 |
| SduI | GDGCHC | 3 cut(s) | 540, 2264, 2870 |
| SfaNI | GCATC | 4 cut(s) | 1756, 2518, 2809, 2945 |
| SfoI | GGCGCC | 1 cut(s) | 1326 |
| Sfr274I | CTCGAG | 3 cut(s) | 539, 879, 2398 |
| SinI | GGWCC | 1 cut(s) | 406 |
| SlaI | CTCGAG | 3 cut(s) | 539, 879, 2398 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1221, 2490 |
| SmlI | CTYRAG | 8 cut(s) | 143, 539, 689, 879, 901, 2225, 2257, 2398 |
| SmoI | CTYRAG | 8 cut(s) | 143, 539, 689, 879, 901, 2225, 2257, 2398 |
| SpeI | ACTAGT | 1 cut(s) | 1619 |
| SseBI | AGGCCT | 2 cut(s) | 1854, 2920 |
| SspDI | GGCGCC | 1 cut(s) | 1324 |
| SspI | AATATT | 1 cut(s) | 1086 |
| SspMI | CTAG | 5 cut(s) | 395, 1308, 1539, 1620, 1866 |
| SstI | GAGCTC | 2 cut(s) | 540, 2264 |
| StuI | AGGCCT | 2 cut(s) | 1854, 2920 |
| StyD4I | CCNGG | 6 cut(s) | 218, 355, 461, 1460, 1681, 2355 |
| StyI | CCWWGG | 2 cut(s) | 650, 3036 |
| TaaI | ACNGT | 7 cut(s) | 328, 788, 1270, 1293, 1965, 2027, 2492 |
| TaiI | ACGT | 2 cut(s) | 2520, 2697 |
| TatI | WGTACW | 4 cut(s) | 399, 555, 2337, 2968 |
| TauI | GCSGC | 1 cut(s) | 2500 |
| TfiI | GAWTC | 9 cut(s) | 544, 802, 890, 1213, 1567, 1901, 2036, 2047, 2543 |
| Tru1I | TTAA | 5 cut(s) | 155, 932, 974, 1526, 1626 |
| Tru9I | TTAA | 5 cut(s) | 155, 932, 974, 1526, 1626 |
| TscAI | CASTG | 6 cut(s) | 218, 1228, 2314, 2457, 2770, 2902 |
| TseFI | GTSAC | 6 cut(s) | 250, 1611, 2593, 2789, 2849, 3061 |
| TseI | GCWGC | 6 cut(s) | 1254, 1820, 2298, 2368, 2459, 2958 |
| Tsp45I | GTSAC | 6 cut(s) | 250, 1611, 2593, 2789, 2849, 3061 |
| TspGWI | ACGGA | 3 cut(s) | 238, 715, 2860 |
| TspRI | CASTG | 6 cut(s) | 218, 1228, 2314, 2457, 2770, 2902 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1100 |
| VpaK11BI | GGWCC | 1 cut(s) | 406 |
| XapI | RAATTY | 7 cut(s) | 228, 715, 829, 1728, 2071, 2088, 2209 |
| XceI | RCATGY | 3 cut(s) | 628, 2276, 2996 |
| XhoI | CTCGAG | 3 cut(s) | 539, 879, 2398 |
| XmiI | GTMKAC | 2 cut(s) | 378, 2331 |
| XmnI | GAANNNNTTC | 1 cut(s) | 566 |
| XspI | CTAG | 5 cut(s) | 395, 1308, 1539, 1620, 1866 |
| ZrmI | AGTACT | 3 cut(s) | 401, 2339, 2970 |