Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1370 |
| AccBSI | CCGCTC | 1 cut(s) | 755 |
| AccI | GTMKAC | 1 cut(s) | 1158 |
| AccII | CGCG | 2 cut(s) | 242, 717 |
| AciI | CCGC | 6 cut(s) | 222, 225, 240, 242, 755, 937 |
| AclI | AACGTT | 1 cut(s) | 195 |
| AclWI | GGATC | 4 cut(s) | 415, 428, 777, 1039 |
| AcoI | YGGCCR | 1 cut(s) | 934 |
| AcsI | RAATTY | 1 cut(s) | 128 |
| AcuI | CTGAAG | 1 cut(s) | 551 |
| AcyI | GRCGYC | 1 cut(s) | 1367 |
| AfaI | GTAC | 3 cut(s) | 320, 406, 491 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 664, 991 |
| AflIII | ACRYGT | 3 cut(s) | 306, 373, 715 |
| AgsI | TTSAA | 1 cut(s) | 701 |
| AjiI | CACGTC | 1 cut(s) | 307 |
| AjnI | CCWGG | 4 cut(s) | 634, 1063, 1123, 1202 |
| AluBI | AGCT | 7 cut(s) | 163, 236, 382, 674, 869, 919, 1243 |
| AluI | AGCT | 7 cut(s) | 163, 236, 382, 674, 869, 919, 1243 |
| Alw21I | GWGCWC | 3 cut(s) | 165, 871, 1245 |
| Alw26I | GTCTC | 3 cut(s) | 273, 958, 1001 |
| AlwI | GGATC | 4 cut(s) | 415, 428, 777, 1039 |
| Ama87I | CYCGRG | 1 cut(s) | 750 |
| AoxI | GGCC | 5 cut(s) | 329, 694, 737, 845, 934 |
| ApeKI | GCWGC | 3 cut(s) | 440, 449, 916 |
| ApoI | RAATTY | 1 cut(s) | 128 |
| AspS9I | GGNCC | 6 cut(s) | 186, 738, 796, 845, 1061, 1337 |
| AsuHPI | GGTGA | 4 cut(s) | 379, 604, 845, 1171 |
| AvaI | CYCGRG | 1 cut(s) | 750 |
| AvaII | GGWCC | 4 cut(s) | 186, 796, 1061, 1337 |
| BamHI | GGATCC | 1 cut(s) | 420 |
| BanII | GRGCYC | 4 cut(s) | 165, 685, 871, 1245 |
| BauI | CACGAG | 1 cut(s) | 164 |
| BbsI | GAAGAC | 1 cut(s) | 1168 |
| Bbv12I | GWGCWC | 3 cut(s) | 165, 871, 1245 |
| BbvI | GCAGC | 3 cut(s) | 427, 461, 928 |
| BccI | CCATC | 9 cut(s) | 126, 411, 525, 624, 667, 686, 740, 817, 1021 |
| BciT130I | CCWGG | 4 cut(s) | 636, 1065, 1125, 1204 |
| BclI | TGATCA | 1 cut(s) | 1086 |
| BcoDI | GTCTC | 3 cut(s) | 273, 958, 1001 |
| BfaI | CTAG | 3 cut(s) | 414, 686, 1274 |
| BfmI | CTRYAG | 1 cut(s) | 810 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 736 |
| BisI | GCNGC | 5 cut(s) | 223, 441, 450, 917, 937 |
| BlsI | GCNGC | 5 cut(s) | 224, 442, 451, 918, 938 |
| BmcAI | AGTACT | 1 cut(s) | 406 |
| Bme1390I | CCNGG | 4 cut(s) | 636, 1065, 1125, 1204 |
| Bme18I | GGWCC | 4 cut(s) | 186, 796, 1061, 1337 |
| BmeT110I | CYCGRG | 1 cut(s) | 750 |
| BmgBI | CACGTC | 1 cut(s) | 307 |
| BmgT120I | GGNCC | 6 cut(s) | 186, 738, 796, 845, 1061, 1337 |
| BmiI | GGNNCC | 3 cut(s) | 422, 798, 846 |
| BmrFI | CCNGG | 4 cut(s) | 636, 1065, 1125, 1204 |
| BmrI | ACTGGG | 2 cut(s) | 908, 980 |
| BmsI | GCATC | 3 cut(s) | 247, 516, 1053 |
| BmuI | ACTGGG | 2 cut(s) | 908, 980 |
| BpiI | GAAGAC | 1 cut(s) | 1168 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 771, 803, 867, 899 |
| Bpu10I | CCTNAGC | 1 cut(s) | 77 |
| BpuEI | CTTGAG | 1 cut(s) | 74 |
| BsaHI | GRCGYC | 1 cut(s) | 1367 |
| BsaJI | CCNNGG | 4 cut(s) | 240, 462, 635, 765 |
| BsaWI | WCCGGW | 1 cut(s) | 1282 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 32, 62, 938, 968 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 664, 991 |
| Bse118I | RCCGGY | 1 cut(s) | 218 |
| Bse1I | ACTGG | 3 cut(s) | 903, 966, 986 |
| Bse3DI | GCAATG | 2 cut(s) | 444, 1147 |
| BseBI | CCWGG | 4 cut(s) | 636, 1065, 1125, 1204 |
| BseDI | CCNNGG | 4 cut(s) | 240, 462, 635, 765 |
| BseGI | GGATG | 8 cut(s) | 262, 517, 616, 631, 1032, 1243, 1257, 1350 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 664, 991 |
| BseMI | GCAATG | 2 cut(s) | 444, 1147 |
| BseMII | CTCAG | 4 cut(s) | 68, 171, 790, 1248 |
| BseNI | ACTGG | 3 cut(s) | 903, 966, 986 |
| BseXI | GCAGC | 3 cut(s) | 427, 461, 928 |
| BsgI | GTGCAG | 1 cut(s) | 188 |
| Bsh1236I | CGCG | 2 cut(s) | 242, 717 |
| BshFI | GGCC | 5 cut(s) | 331, 696, 739, 847, 936 |
| BsiHKAI | GWGCWC | 3 cut(s) | 165, 871, 1245 |
| BsiHKCI | CYCGRG | 1 cut(s) | 750 |
| BsiSI | CCGG | 3 cut(s) | 219, 1235, 1283 |
| BslFI | GGGAC | 2 cut(s) | 166, 691 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 664, 991 |
| BsmAI | GTCTC | 3 cut(s) | 273, 958, 1001 |
| BsmBI | CGTCTC | 1 cut(s) | 273 |
| BsmFI | GGGAC | 2 cut(s) | 166, 691 |
| BsmI | GAATGC | 1 cut(s) | 1267 |
| BsnI | GGCC | 5 cut(s) | 331, 696, 739, 847, 936 |
| BsoBI | CYCGRG | 1 cut(s) | 750 |
| Bsp1286I | GDGCHC | 4 cut(s) | 165, 685, 871, 1245 |
| Bsp143I | GATC | 7 cut(s) | 249, 420, 601, 769, 921, 1031, 1086 |
| BspACI | CCGC | 6 cut(s) | 222, 225, 240, 242, 755, 937 |
| BspANI | GGCC | 5 cut(s) | 331, 696, 739, 847, 936 |
| BspCNI | CTCAG | 4 cut(s) | 69, 172, 789, 1249 |
| BspFNI | CGCG | 2 cut(s) | 242, 717 |
| BspLI | GGNNCC | 3 cut(s) | 422, 798, 846 |
| BspMAI | CTGCAG | 1 cut(s) | 814 |
| BspPI | GGATC | 4 cut(s) | 415, 428, 777, 1039 |
| BsrBI | CCGCTC | 1 cut(s) | 755 |
| BsrDI | GCAATG | 2 cut(s) | 444, 1147 |
| BsrFI | RCCGGY | 1 cut(s) | 218 |
| BsrI | ACTGG | 3 cut(s) | 903, 966, 986 |
| BssAI | RCCGGY | 1 cut(s) | 218 |
| BssECI | CCNNGG | 4 cut(s) | 240, 462, 635, 765 |
| BssMI | GATC | 7 cut(s) | 249, 420, 601, 769, 921, 1031, 1086 |
| BssNI | GRCGYC | 1 cut(s) | 1367 |
| BssSI | CACGAG | 1 cut(s) | 164 |
| BssT1I | CCWWGG | 2 cut(s) | 462, 765 |
| Bst2BI | CACGAG | 1 cut(s) | 164 |
| Bst2UI | CCWGG | 4 cut(s) | 636, 1065, 1125, 1204 |
| Bst4CI | ACNGT | 4 cut(s) | 104, 404, 428, 592 |
| Bst6I | CTCTTC | 1 cut(s) | 696 |
| BstACI | GRCGYC | 1 cut(s) | 1367 |
| BstC8I | GCNNGC | 3 cut(s) | 728, 810, 1327 |
| BstDEI | CTNAG | 4 cut(s) | 77, 180, 776, 1257 |
| BstDSI | CCRYGG | 1 cut(s) | 240 |
| BstF5I | GGATG | 8 cut(s) | 262, 517, 616, 631, 1032, 1243, 1257, 1350 |
| BstFNI | CGCG | 2 cut(s) | 242, 717 |
| BstKTI | GATC | 7 cut(s) | 252, 423, 604, 772, 924, 1034, 1089 |
| BstMAI | GTCTC | 3 cut(s) | 273, 958, 1001 |
| BstMBI | GATC | 7 cut(s) | 249, 420, 601, 769, 921, 1031, 1086 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 446, 449, 458, 680, 723, 736, 1240 |
| BstNI | CCWGG | 4 cut(s) | 636, 1065, 1125, 1204 |
| BstNSI | RCATGY | 2 cut(s) | 377, 1363 |
| BstSCI | CCNGG | 4 cut(s) | 634, 1063, 1123, 1202 |
| BstSFI | CTRYAG | 1 cut(s) | 810 |
| BstUI | CGCG | 2 cut(s) | 242, 717 |
| BstV1I | GCAGC | 3 cut(s) | 427, 461, 928 |
| BstV2I | GAAGAC | 1 cut(s) | 1168 |
| BstX2I | RGATCY | 2 cut(s) | 420, 1031 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1210 |
| BstYI | RGATCY | 2 cut(s) | 420, 1031 |
| BsuRI | GGCC | 5 cut(s) | 331, 696, 739, 847, 936 |
| BtgI | CCRYGG | 1 cut(s) | 240 |
| BtrI | CACGTC | 1 cut(s) | 307 |
| BtsCI | GGATG | 8 cut(s) | 262, 517, 616, 631, 1032, 1243, 1257, 1350 |
| BtsIMutI | CAGTG | 2 cut(s) | 433, 959 |
| Cac8I | GCNNGC | 3 cut(s) | 728, 810, 1327 |
| Cfr10I | RCCGGY | 1 cut(s) | 218 |
| Cfr13I | GGNCC | 6 cut(s) | 186, 738, 796, 845, 1061, 1337 |
| Cfr42I | CCGCGG | 1 cut(s) | 243 |
| CseI | GACGC | 1 cut(s) | 6 |
| Csp6I | GTAC | 3 cut(s) | 319, 405, 490 |
| CviAII | CATG | 4 cut(s) | 364, 374, 624, 1360 |
| CviQI | GTAC | 3 cut(s) | 319, 405, 490 |
| DdeI | CTNAG | 4 cut(s) | 77, 180, 776, 1257 |
| DpnI | GATC | 7 cut(s) | 251, 422, 603, 771, 923, 1033, 1088 |
| DpnII | GATC | 7 cut(s) | 249, 420, 601, 769, 921, 1031, 1086 |
| DraI | TTTAAA | 1 cut(s) | 336 |
| EaeI | YGGCCR | 1 cut(s) | 934 |
| Eam1104I | CTCTTC | 1 cut(s) | 696 |
| EarI | CTCTTC | 1 cut(s) | 696 |
| EciI | GGCGGA | 1 cut(s) | 240 |
| Ecl136II | GAGCTC | 3 cut(s) | 163, 869, 1243 |
| Eco130I | CCWWGG | 2 cut(s) | 462, 765 |
| Eco24I | GRGCYC | 4 cut(s) | 165, 685, 871, 1245 |
| Eco47I | GGWCC | 4 cut(s) | 186, 796, 1061, 1337 |
| Eco53kI | GAGCTC | 3 cut(s) | 163, 869, 1243 |
| Eco57I | CTGAAG | 1 cut(s) | 551 |
| Eco88I | CYCGRG | 1 cut(s) | 750 |
| EcoICRI | GAGCTC | 3 cut(s) | 163, 869, 1243 |
| EcoO109I | RGGNCCY | 2 cut(s) | 738, 845 |
| EcoRII | CCWGG | 4 cut(s) | 634, 1063, 1123, 1202 |
| EcoT14I | CCWWGG | 2 cut(s) | 462, 765 |
| EcoT38I | GRGCYC | 4 cut(s) | 165, 685, 871, 1245 |
| ErhI | CCWWGG | 2 cut(s) | 462, 765 |
| Esp3I | CGTCTC | 1 cut(s) | 273 |
| FaeI | CATG | 4 cut(s) | 367, 377, 627, 1363 |
| FaqI | GGGAC | 2 cut(s) | 166, 691 |
| FatI | CATG | 4 cut(s) | 363, 373, 623, 1359 |
| FbaI | TGATCA | 1 cut(s) | 1086 |
| FblI | GTMKAC | 1 cut(s) | 1158 |
| Fnu4HI | GCNGC | 5 cut(s) | 223, 441, 450, 917, 937 |
| FokI | GGATG | 8 cut(s) | 269, 504, 603, 618, 1039, 1250, 1264, 1337 |
| FriOI | GRGCYC | 4 cut(s) | 165, 685, 871, 1245 |
| Fsp4HI | GCNGC | 5 cut(s) | 223, 441, 450, 917, 937 |
| FspBI | CTAG | 3 cut(s) | 414, 686, 1274 |
| GluI | GCNGC | 5 cut(s) | 223, 441, 450, 917, 937 |
| HaeIII | GGCC | 5 cut(s) | 331, 696, 739, 847, 936 |
| HapII | CCGG | 3 cut(s) | 219, 1235, 1283 |
| HgaI | GACGC | 1 cut(s) | 6 |
| Hin1I | GRCGYC | 1 cut(s) | 1367 |
| Hin1II | CATG | 4 cut(s) | 367, 377, 627, 1363 |
| HinfI | GANTC | 4 cut(s) | 202, 704, 878, 965 |
| HpaII | CCGG | 3 cut(s) | 219, 1235, 1283 |
| HphI | GGTGA | 4 cut(s) | 379, 604, 845, 1171 |
| Hpy166II | GTNNAC | 2 cut(s) | 321, 1159 |
| Hpy188I | TCNGA | 6 cut(s) | 207, 570, 779, 970, 1091, 1258 |
| Hpy188III | TCNNGA | 2 cut(s) | 91, 1316 |
| Hpy8I | GTNNAC | 2 cut(s) | 321, 1159 |
| Hpy99I | CGWCG | 3 cut(s) | 22, 143, 146 |
| HpyAV | CCTTC | 3 cut(s) | 295, 382, 830 |
| HpyCH4III | ACNGT | 4 cut(s) | 104, 404, 428, 592 |
| HpyCH4IV | ACGT | 4 cut(s) | 195, 306, 1333, 1367 |
| HpyCH4V | TGCA | 8 cut(s) | 169, 395, 449, 529, 812, 1044, 1265, 1346 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 446, 449, 458, 680, 723, 736, 1240 |
| HpyF3I | CTNAG | 4 cut(s) | 77, 180, 776, 1257 |
| HpySE526I | ACGT | 4 cut(s) | 195, 306, 1333, 1367 |
| Hsp92I | GRCGYC | 1 cut(s) | 1367 |
| Hsp92II | CATG | 4 cut(s) | 367, 377, 627, 1363 |
| Ksp22I | TGATCA | 1 cut(s) | 1086 |
| KspI | CCGCGG | 1 cut(s) | 243 |
| Kzo9I | GATC | 7 cut(s) | 249, 420, 601, 769, 921, 1031, 1086 |
| LmnI | GCTCC | 1 cut(s) | 160 |
| Lsp1109I | GCAGC | 3 cut(s) | 427, 461, 928 |
| LweI | GCATC | 3 cut(s) | 247, 516, 1053 |
| MaeI | CTAG | 3 cut(s) | 414, 686, 1274 |
| MaeII | ACGT | 4 cut(s) | 195, 306, 1333, 1367 |
| MaeIII | GTNAC | 3 cut(s) | 586, 650, 1037 |
| MalI | GATC | 7 cut(s) | 251, 422, 603, 771, 923, 1033, 1088 |
| MbiI | CCGCTC | 1 cut(s) | 755 |
| MboI | GATC | 7 cut(s) | 249, 420, 601, 769, 921, 1031, 1086 |
| MboII | GAAGA | 4 cut(s) | 583, 713, 794, 1168 |
| MfeI | CAATTG | 1 cut(s) | 909 |
| MflI | RGATCY | 2 cut(s) | 420, 1031 |
| MhlI | GDGCHC | 4 cut(s) | 165, 685, 871, 1245 |
| MluCI | AATT | 4 cut(s) | 128, 507, 803, 909 |
| MluI | ACGCGT | 1 cut(s) | 715 |
| MlyI | GAGTC | 1 cut(s) | 713 |
| MseI | TTAA | 3 cut(s) | 335, 1269, 1305 |
| MspA1I | CMGCKG | 2 cut(s) | 242, 919 |
| MspI | CCGG | 3 cut(s) | 219, 1235, 1283 |
| MspR9I | CCNGG | 4 cut(s) | 636, 1065, 1125, 1204 |
| MunI | CAATTG | 1 cut(s) | 909 |
| Mva1269I | GAATGC | 1 cut(s) | 1267 |
| MvaI | CCWGG | 4 cut(s) | 636, 1065, 1125, 1204 |
| MvnI | CGCG | 2 cut(s) | 242, 717 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 446, 449, 458, 680, 723, 736, 1240 |
| NdeII | GATC | 7 cut(s) | 249, 420, 601, 769, 921, 1031, 1086 |
| NlaIII | CATG | 4 cut(s) | 367, 377, 627, 1363 |
| NlaIV | GGNNCC | 3 cut(s) | 422, 798, 846 |
| NmeAIII | GCCGAG | 1 cut(s) | 48 |
| NmuCI | GTSAC | 2 cut(s) | 586, 1037 |
| NspI | RCATGY | 2 cut(s) | 377, 1363 |
| PciI | ACATGT | 1 cut(s) | 373 |
| PctI | GAATGC | 1 cut(s) | 1267 |
| PfeI | GAWTC | 3 cut(s) | 202, 878, 965 |
| PfoI | TCCNGGA | 1 cut(s) | 1123 |
| PkrI | GCNGC | 5 cut(s) | 224, 442, 451, 918, 938 |
| PleI | GAGTC | 1 cut(s) | 712 |
| PpsI | GAGTC | 1 cut(s) | 712 |
| PscI | ACATGT | 1 cut(s) | 373 |
| Psp124BI | GAGCTC | 3 cut(s) | 165, 871, 1245 |
| Psp1406I | AACGTT | 1 cut(s) | 195 |
| Psp6I | CCWGG | 4 cut(s) | 634, 1063, 1123, 1202 |
| PspGI | CCWGG | 4 cut(s) | 634, 1063, 1123, 1202 |
| PspN4I | GGNNCC | 3 cut(s) | 422, 798, 846 |
| PspPI | GGNCC | 6 cut(s) | 186, 738, 796, 845, 1061, 1337 |
| PstI | CTGCAG | 1 cut(s) | 814 |
| PsuI | RGATCY | 2 cut(s) | 420, 1031 |
| PvuII | CAGCTG | 1 cut(s) | 919 |
| RsaI | GTAC | 3 cut(s) | 320, 406, 491 |
| RsaNI | GTAC | 3 cut(s) | 319, 405, 490 |
| SacI | GAGCTC | 3 cut(s) | 165, 871, 1245 |
| SacII | CCGCGG | 1 cut(s) | 243 |
| SaqAI | TTAA | 3 cut(s) | 335, 1269, 1305 |
| SatI | GCNGC | 5 cut(s) | 223, 441, 450, 917, 937 |
| Sau3AI | GATC | 7 cut(s) | 249, 420, 601, 769, 921, 1031, 1086 |
| Sau96I | GGNCC | 6 cut(s) | 186, 738, 796, 845, 1061, 1337 |
| ScaI | AGTACT | 1 cut(s) | 406 |
| SchI | GAGTC | 1 cut(s) | 713 |
| ScrFI | CCNGG | 4 cut(s) | 636, 1065, 1125, 1204 |
| SduI | GDGCHC | 4 cut(s) | 165, 685, 871, 1245 |
| SfaNI | GCATC | 3 cut(s) | 247, 516, 1053 |
| SfcI | CTRYAG | 1 cut(s) | 810 |
| Sfr303I | CCGCGG | 1 cut(s) | 243 |
| SgrBI | CCGCGG | 1 cut(s) | 243 |
| SinI | GGWCC | 4 cut(s) | 186, 796, 1061, 1337 |
| SmlI | CTYRAG | 1 cut(s) | 89 |
| SmoI | CTYRAG | 1 cut(s) | 89 |
| Sse9I | AATT | 4 cut(s) | 128, 507, 803, 909 |
| SsiI | CCGC | 6 cut(s) | 222, 225, 240, 242, 755, 937 |
| SspMI | CTAG | 3 cut(s) | 414, 686, 1274 |
| SstI | GAGCTC | 3 cut(s) | 165, 871, 1245 |
| StyD4I | CCNGG | 4 cut(s) | 634, 1063, 1123, 1202 |
| StyI | CCWWGG | 2 cut(s) | 462, 765 |
| TaaI | ACNGT | 4 cut(s) | 104, 404, 428, 592 |
| TaiI | ACGT | 4 cut(s) | 198, 309, 1336, 1370 |
| TaqI | TCGA | 3 cut(s) | 471, 789, 1296 |
| TasI | AATT | 4 cut(s) | 128, 507, 803, 909 |
| TatI | WGTACW | 3 cut(s) | 318, 404, 489 |
| TauI | GCSGC | 2 cut(s) | 225, 939 |
| TfiI | GAWTC | 3 cut(s) | 202, 878, 965 |
| Tru1I | TTAA | 3 cut(s) | 335, 1269, 1305 |
| Tru9I | TTAA | 3 cut(s) | 335, 1269, 1305 |
| TscAI | CASTG | 2 cut(s) | 433, 966 |
| TseFI | GTSAC | 2 cut(s) | 586, 1037 |
| TseI | GCWGC | 3 cut(s) | 440, 449, 916 |
| Tsp45I | GTSAC | 2 cut(s) | 586, 1037 |
| TspDTI | ATGAA | 2 cut(s) | 352, 620 |
| TspGWI | ACGGA | 1 cut(s) | 53 |
| TspRI | CASTG | 2 cut(s) | 433, 966 |
| VpaK11BI | GGWCC | 4 cut(s) | 186, 796, 1061, 1337 |
| XapI | RAATTY | 1 cut(s) | 128 |
| XceI | RCATGY | 2 cut(s) | 377, 1363 |
| XmiI | GTMKAC | 1 cut(s) | 1158 |
| XspI | CTAG | 3 cut(s) | 414, 686, 1274 |
| ZraI | GACGTC | 1 cut(s) | 1368 |
| ZrmI | AGTACT | 1 cut(s) | 406 |