Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001321

Physical Location & Seq

Genomic Coordinates Forward (+)

5179649 .. 5183336

Gene Structure 3688 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016266

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3027 bp

ATGACTACACCAACCCCAAAACCCTCTCTCATCTTCCTCATCTTCTTCGTCTTCTTCATCCATGCAAACTCTCAGTCCCTTCCAGGCAAGGAATTATCAGTCCTGCTTGAATTAAAGTCATATTGGCTATATCCACCAGCTCTCAGTCATTGGACTTCATCATCAAACTCTGGTTCCCACTGTAACTGGCCTGAGATCAACTGCACCGGAAACTCCGTCACCGGATTGAATCTCAACACCGTAAACCTCACCCTTGAAGTTCCACCCTTCATTTGTGACCTCAACAACCTCACACACCTTGACCTTGGTAATAACTACCTCCCGGGAGATTTTCCCTTATCTCTCTACAACTGCTCCAAGCTAGAGTATCTTGACCTCTCGCAAAACTACTTTGCGGGTACAATTCCTGGTCACATCGACAGACTGCCTAAGCTTAAGATCCTCAACCTCGCTGGCAACAACTTCTCCGGTGACATTCCGGCGTCTATTGGGAACTTACTGGAGCTGGCTACTTTGGATATATACATGAACCAGTTCAACGGCTCTGTCCCAGCTGAAATAGGTAACCTCTCCAACCTTGAGGAACTCTGGCTCTCTTGGCTTCCCAAAATGGCGCCATGGAAGCTGCCTTTTAATTTCACCAAGTTGAAAAAGATGAAGATATTGAAGATACGCGAAGCAAATTTGATTGGGGAAATACCAGAATCTATTGGAGAATTGGAAGCACTTGAAATATTGGATTTGGCGCAGAACAAGTTGAGCGGGAATATACCAAGCGGTGTCTTTCTGTTGAAGAATTTGAGCATTATATATCTGTTCAAGAACAGGCTTTCAGGGGAGATTCCTCAAGTGGTTGAAGCACTCAACTTGAGCATCATTGACATCTCCGAAAACAATCTCACTGGGCCGATACCAGAAGGCTTCGGAAACCTTACCAAGTTAACAGATATGAGTTTGTTTTTAAATCATATTTCAGGGGAGATTCCAGAGAGCATTGGCCGCCTGCCAAATCTTGTGATTTTCAAAATGTTCAACATGAACCTGTCAGGAACAATTCCTCCAGAGCTTGGAAAGCATTCGCTGCTCGAAGATTTCCAGGTTTCGGTGAATAGGCTTACCGGAAAGCTGCCAGACGGCTTGTGTAAAAATGGTAAGCTTGTGGGAGTTGTTGCTTATGAGAATAGTCTCACTGGGGAGCTACCAAGCTCTCTAGGAAATTGTGATAGTCTGCTGATAGTTACAGTTTATGATAACATGTTGTCTGGGAACATTCCTAGTGGTATGTGGAATGCACTGAATTTGAGTTATGTGATGATGAGCAACAATTCGTTTACGGGTGAGCTTCCTGAGAAGATGTCACTTAATCTTCAAAGGCTGGAAATCCGAGACAACAAGTTTTCAGGTCAGATTCCAAGAGGGGTGTCTTTGTGTAAGAATCTGCAAGTTTTTGATGCTGGTAACAACCTCTTCAATGGTAGTATTCCTCGGGAATTAACTGCTCTTCCGAAGTTGGCGAGTCTTCTCCTTCATCAGAATCAGCTTAGCGGGCCACTTCCATCAGATATTGTATCATGGAAGTCACTAAACACTCTTGATCTGCGTAGAAATCAACTCTCCGGACCAATTCCAGATATACTTGGTGGTTTGCCCGCCCTTACTGAGTTGGACCTTTCTGAAAACCAGTTTTCAGGTCAAATTCCAAATCAAATTGGCCATCTGAAACTCAACAAGTTCAACCTCTCTTCAAATCTCCTCACTGGCGAGATCCCAATTTCATTTGAAAACCCTGCATATGATACAAGCTTCTTGAACAATACCAATCTTTGTGCAAGCACCAGTGGTGTGAATCTCAATATATGCTCTCAATCGCCAAAGTCCAGCAAGATTTCAATTCGGTCTCTTGCTTTGATCTTAAGTCTAAGCGCTGTGTTGTTCGTGTTGGTTTTGTCCTTATTAATCTTCTTTGTTAGAGGTTACTTGAGGAGGAAGGATGGATCAGACGCAGACTGGAAGCTCGTTGCATTTCAGAGGTTGAATTTCACCATCTCACAAATCTTGTCAGGGTTGACAGAAAGCAATGTGATTGGAAGTGGTGGATCAGGGAGGGTCTATCGTGTACCTGTGAATCGTACAGGTGATGTTGTAGCTGCGAAGAGGATTTGGACCAACAAGAAGATCATGGAGGAGAGACTTGAAAAAGAGTTCCTTGCAGAAGTCAAGATACTGAGCTCAATTCGACATGCCAACATAGTGAAGCTAATGTGCTGCATATCCAGTGAGACTTCAAAACTTCTTGTATATGAGTACTTAGAAAATCGAAGCCTAGATCGATGGTTGCACAAGAAAAACAGGGGCAACCTCTCAAGTTCAGTTGTTCTGGACTGGCCTAAGAGGTTGCAGATTGCAGTGGGAGCTGCTAAGGGCCTCTGCTATATGCATCATGACTGTGTTCCACCCGTTGTGCATCGAGACGTGAAATCAAGTAACATTTTATTGGACTCTGATTTCAACGCAAAAATTGCAGATTTTGGTCTGGCCAAGATGTTGGTCAAGAACGGAGACCTTGCCACAATGTCAGCTGTTGCTGGTTCCTTTGGCTACATGGCTCCAGAATATGCACATACCGGAAGAGTGAATGAAAAGATCGATGTATATAGTTTCGGGGTCATCCTGCTGGAGTTGGCAACTGGTAGGGAACCCCATGAAGGTGATGAAAACACTTCTCTCGCCCAATGGGCGTGGTGCCGTGCTCAAGAAGGCAAACCTATCGCCAGTGCTCTGGACCAGGACGTCAAAGAACCCTGTTACTTGGATGAAATGTGCGCTGTTTTCAGACTCGGCATCAGGTGCACCGAGACACTTCCTACAAATAGGCCTTCTATGAAGAAGGTTCTGCAGATCTTGGTTCGAACTTGTCCTCCACCAGTTCGCAGGGAAAAGACTGAATATGCCGCTGCTCCTCTCCTCAAGAACTCGAGGCGCGAGAGAGTTTTGGAAGATCAAGATGGTTTGGCAACGAATGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0010015 GO:0010101 GO:0010102 GO:0010468 GO:0019222 GO:0022622 GO:0032501 GO:0032502 GO:0048364 GO:0048527 GO:0048528 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0065007 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1009

Amino Acids

111.41

Weight (kDa)

6.78

Isoelectric Point (pI)

38.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	96 - 155	7.2e-10	Leucine rich repeat
LRR_14	PF23598	115 - 224	8.5e-10	Leucine-rich repeat region
LRR_14	PF23598	214 - 276	6e-07	Leucine-rich repeat region
LRR_8	PF13855	527 - 585	1.9e-06	Leucine rich repeat
Pkinase	PF00069	691 - 966	2.7e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	692 - 967	7e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0001321)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25930
fragaria_vesca	FvH4_1g06180 FvH4_1g06180 FvH4_1g06220 FvH4_1g06260 FvH4_1g07450 FvH4_1g07450 FvH4_1g07450 FvH4_2g35260
malus_domestica	MD02G1066200.v1.1 MD02G1066300.v1.1 MD02G1066400.v1.1 MD08G1110500.v1.1 MD15G1196900.v1.1
prunus_persica	Prupe.1G444700_v2.0.a1 Prupe.7G218900_v2.0.a1 Prupe.7G219100_v2.0.a1 Prupe.7G219300_v2.0.a1 Prupe.7G219300_v2.0.a1 Prupe.7G219400_v2.0.a1 Prupe.7G219500_v2.0.a1
pyrus_communis	pycom02g05250 pycom08g08890 pycom08g09200 pycom15g08430 pycom15g17590
rosa_chinensis	RchiOBHm_Chr2g0091961 RchiOBHm_Chr6g0306681
rosa_laevigata	RLG00000010774 RLG00000016262 RLG00000016266
rosa_multiflora	Rmu_co7980064.1_g000001 Rmu_sc0005201.1_g000003 Rmu_sc0009216.1_g000004 Rmu_sc0014758.1_g000001 Rmu_sc0017125.1_g000005 Rmu_sc0017648.1_g000001 Rmu_sc0017649.1_g000001 Rmu_sc0017785.1_g000010 Rmu_sc0017785.1_g000011
rosa_roxburghii	Rroxscaffold_2G00149580 Rroxscaffold_7G00161550
rosa_rugosa	Rorug02G0022000 Rorug06G0351600
rosa_samantha	Rh2AG067500 Rh2BG066800 Rh2CG068100 Rh2DG066600 Rh6AG463900 Rh6BG437100 Rh6CG478000 Rh6DG464800
rosa_wichuraiana	Rw2G005350 Rw6G040410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2789
AatII	GACGTC	1 cut(s)	2793
AccB1I	GGYRCC	2 cut(s)	613, 2742
AccB7I	CCANNNNNTGG	1 cut(s)	1067
AccBSI	CCGCTC	1 cut(s)	762
AccII	CGCG	2 cut(s)	675, 2982
AccIII	TCCGGA	1 cut(s)	1616
AciI	CCGC	7 cut(s)	395, 762, 777, 1000, 1545, 1650, 2952
AclWI	GGATC	4 cut(s)	433, 1759, 2002, 2104
AcoI	YGGCCR	3 cut(s)	997, 1711, 2535
AcsI	RAATTY	5 cut(s)	682, 796, 1297, 1695, 2035
AcyI	GRCGYC	3 cut(s)	482, 614, 2790
AfaI	GTAC	4 cut(s)	400, 2118, 2131, 2306
AfeI	AGCGCT	1 cut(s)	1924
AfiI	CCNNNNNNNGG	3 cut(s)	1067, 1102, 2705
AflII	CTTAAG	2 cut(s)	434, 1912
AflIII	ACRYGT	1 cut(s)	1254
AjiI	CACGTC	1 cut(s)	2473
AjnI	CCWGG	4 cut(s)	82, 406, 1095, 2784
AloI	GAACNNNNNNTCC	4 cut(s)	1042, 1074, 2902, 2934
Alw21I	GWGCWC	4 cut(s)	2231, 2752, 2779, 2852
Alw26I	GTCTC	8 cut(s)	1190, 1380, 1902, 2182, 2273, 2463, 2553, 2849
Alw44I	GTGCAC	1 cut(s)	2848
AlwI	GGATC	4 cut(s)	433, 1759, 2002, 2104
Ama87I	CYCGRG	3 cut(s)	322, 1485, 2974
Aor13HI	TCCGGA	1 cut(s)	1616
Aor51HI	AGCGCT	1 cut(s)	1924
AoxI	GGCC	9 cut(s)	188, 905, 997, 1547, 1711, 2384, 2422, 2535, 2873
ApaLI	GTGCAC	1 cut(s)	2848
ApeKI	GCWGC	7 cut(s)	625, 1081, 1126, 2147, 2265, 2414, 2954
ApoI	RAATTY	5 cut(s)	682, 796, 1297, 1695, 2035
ArsI	GACNNNNNNTTYG	2 cut(s)	59, 91
AseI	ATTAAT	1 cut(s)	1955
Asp700I	GAANNNNTTC	2 cut(s)	533, 1075
AspLEI	GCGC	5 cut(s)	616, 748, 1925, 2825, 2982
AspS9I	GGNCC	7 cut(s)	905, 1547, 1619, 1666, 2163, 2422, 2782
AsuC2I	CCSGG	2 cut(s)	323, 324
AsuHPI	GGTGA	9 cut(s)	211, 241, 482, 631, 1117, 1349, 2032, 2147, 2720
AsuII	TTCGAA	1 cut(s)	2908
AvaI	CYCGRG	3 cut(s)	322, 1485, 2974
AvaII	GGWCC	4 cut(s)	1619, 1666, 2163, 2782
BaeGI	GKGCMC	1 cut(s)	2852
BalI	TGGCCA	2 cut(s)	1713, 2537
BanI	GGYRCC	2 cut(s)	613, 2742
BanII	GRGCYC	1 cut(s)	2231
BarI	GAAGNNNNNNTAC	2 cut(s)	2205, 2237
BbsI	GAAGAC	2 cut(s)	43, 1511
Bbv12I	GWGCWC	4 cut(s)	2231, 2752, 2779, 2852
BbvI	GCAGC	7 cut(s)	612, 1068, 1113, 2134, 2252, 2401, 2941
BccI	CCATC	6 cut(s)	1564, 1722, 1985, 2051, 2325, 2999
BceAI	ACGGC	3 cut(s)	556, 1150, 2730
BcgI	CGANNNNNNTGC	2 cut(s)	2749, 2783
BciT130I	CCWGG	4 cut(s)	84, 408, 1097, 2786
BcnI	CCSGG	2 cut(s)	323, 324
BcoDI	GTCTC	8 cut(s)	1190, 1380, 1902, 2182, 2273, 2463, 2553, 2849
BfaI	CTAG	4 cut(s)	362, 1211, 1275, 2324
BfmI	CTRYAG	1 cut(s)	2894
BfoI	RGCGCY	2 cut(s)	617, 1926
BfrI	CTTAAG	2 cut(s)	434, 1912
BglI	GCCNNNNNGGC	1 cut(s)	2735
BglII	AGATCT	1 cut(s)	2898
BisI	GCNGC	9 cut(s)	626, 1000, 1082, 1127, 2148, 2266, 2415, 2952, 2955
BlpI	GCTNAGC	1 cut(s)	1541
BlsI	GCNGC	9 cut(s)	627, 1001, 1083, 1128, 2149, 2267, 2416, 2953, 2956
BmcAI	AGTACT	1 cut(s)	2306
Bme1390I	CCNGG	6 cut(s)	84, 323, 324, 408, 1097, 2786
Bme18I	GGWCC	4 cut(s)	1619, 1666, 2163, 2782
BmeT110I	CYCGRG	3 cut(s)	322, 1485, 2974
BmgBI	CACGTC	1 cut(s)	2473
BmgT120I	GGNCC	7 cut(s)	905, 1547, 1619, 1666, 2163, 2422, 2782
BmiI	GGNNCC	6 cut(s)	175, 615, 2590, 2607, 2697, 2744
BmrFI	CCNGG	6 cut(s)	84, 323, 324, 408, 1097, 2786
BmrI	ACTGGG	2 cut(s)	912, 1200
BmsI	GCATC	5 cut(s)	882, 1441, 2446, 2473, 2850
BmuI	ACTGGG	2 cut(s)	912, 1200
BpiI	GAAGAC	2 cut(s)	43, 1511
BpmI	CTGGAG	4 cut(s)	521, 1044, 2592, 2696
Bpu10I	CCTNAGC	2 cut(s)	429, 2418
Bpu1102I	GCTNAGC	1 cut(s)	1541
Bpu14I	TTCGAA	1 cut(s)	2908
BpuEI	CTTGAG	7 cut(s)	599, 831, 889, 1999, 2347, 2736, 2951
BpuMI	CCSGG	2 cut(s)	323, 324
Bsa29I	ATCGAT	2 cut(s)	2329, 2646
BsaHI	GRCGYC	3 cut(s)	482, 614, 2790
BsaI	GGTCTC	2 cut(s)	1902, 2553
BsaJI	CCNNGG	4 cut(s)	304, 322, 617, 1484
BsaWI	WCCGGW	6 cut(s)	206, 221, 467, 1118, 1616, 2624
BsaXI	ACNNNNNCTCC	8 cut(s)	338, 368, 449, 479, 1042, 1072, 2902, 2932
Bsc4I	CCNNNNNNNGG	3 cut(s)	1067, 1102, 2705
Bse3DI	GCAATG	1 cut(s)	2083
BseAI	TCCGGA	1 cut(s)	1616
BseBI	CCWGG	4 cut(s)	84, 408, 1097, 2786
BseCI	ATCGAT	2 cut(s)	2329, 2646
BseDI	CCNNGG	4 cut(s)	304, 322, 617, 1484
BseGI	GGATG	4 cut(s)	57, 1996, 2667, 2818
BseLI	CCNNNNNNNGG	3 cut(s)	1067, 1102, 2705
BseMI	GCAATG	1 cut(s)	2083
BseMII	CTCAG	6 cut(s)	86, 157, 183, 1338, 1650, 2216
BseRI	GAGGAG	5 cut(s)	1742, 1996, 2198, 2949, 2954
BseSI	GKGCMC	1 cut(s)	2852
BseXI	GCAGC	7 cut(s)	612, 1068, 1113, 2134, 2252, 2401, 2941
BseYI	CCCAGC	1 cut(s)	550
BsgI	GTGCAG	1 cut(s)	187
Bsh1236I	CGCG	2 cut(s)	675, 2982
BshFI	GGCC	9 cut(s)	190, 907, 999, 1549, 1713, 2386, 2424, 2537, 2875
BshNI	GGYRCC	2 cut(s)	613, 2742
BshVI	ATCGAT	2 cut(s)	2329, 2646
BsiHKAI	GWGCWC	4 cut(s)	2231, 2752, 2779, 2852
BsiHKCI	CYCGRG	3 cut(s)	322, 1485, 2974
BsiSI	CCGG	8 cut(s)	207, 222, 323, 468, 479, 1119, 1617, 2625
BslFI	GGGAC	2 cut(s)	61, 533
BslI	CCNNNNNNNGG	3 cut(s)	1067, 1102, 2705
BsmAI	GTCTC	8 cut(s)	1190, 1380, 1902, 2182, 2273, 2463, 2553, 2849
BsmBI	CGTCTC	1 cut(s)	2463
BsmFI	GGGAC	2 cut(s)	61, 533
BsmI	GAATGC	2 cut(s)	1075, 1294
BsnI	GGCC	9 cut(s)	190, 907, 999, 1549, 1713, 2386, 2424, 2537, 2875
Bso31I	GGTCTC	2 cut(s)	1902, 2553
BsoBI	CYCGRG	3 cut(s)	322, 1485, 2974
Bsp119I	TTCGAA	1 cut(s)	2908
Bsp1286I	GDGCHC	4 cut(s)	2231, 2752, 2779, 2852
Bsp13I	TCCGGA	1 cut(s)	1616
Bsp1720I	GCTNAGC	1 cut(s)	1541
Bsp19I	CCATGG	1 cut(s)	617
BspACI	CCGC	7 cut(s)	395, 762, 777, 1000, 1545, 1650, 2952
BspANI	GGCC	9 cut(s)	190, 907, 999, 1549, 1713, 2386, 2424, 2537, 2875
BspCNI	CTCAG	6 cut(s)	85, 156, 184, 1339, 1651, 2217
BspDI	ATCGAT	2 cut(s)	2329, 2646
BspEI	TCCGGA	1 cut(s)	1616
BspFNI	CGCG	2 cut(s)	675, 2982
BspHI	TCATGA	1 cut(s)	2440
BspLI	GGNNCC	6 cut(s)	175, 615, 2590, 2607, 2697, 2744
BspMAI	CTGCAG	1 cut(s)	2898
BspPI	GGATC	4 cut(s)	433, 1759, 2002, 2104
BspQI	GCTCTTC	1 cut(s)	1506
BspT104I	TTCGAA	1 cut(s)	2908
BspT107I	GGYRCC	2 cut(s)	613, 2742
BspTI	CTTAAG	2 cut(s)	434, 1912
BspTNI	GGTCTC	2 cut(s)	1902, 2553
BsrBI	CCGCTC	1 cut(s)	762
BsrDI	GCAATG	1 cut(s)	2083
BssECI	CCNNGG	4 cut(s)	304, 322, 617, 1484
BssNI	GRCGYC	3 cut(s)	482, 614, 2790
BssT1I	CCWWGG	2 cut(s)	304, 617
Bst2UI	CCWGG	4 cut(s)	84, 408, 1097, 2786
Bst4CI	ACNGT	4 cut(s)	182, 241, 1243, 2447
Bst6I	CTCTTC	5 cut(s)	1472, 1506, 1747, 2147, 2623
BstACI	GRCGYC	3 cut(s)	482, 614, 2790
BstAFI	CTTAAG	2 cut(s)	434, 1912
BstAPI	GCANNNNNTGC	3 cut(s)	1081, 2519, 2847
BstBI	TTCGAA	1 cut(s)	2908
BstC8I	GCNNGC	6 cut(s)	454, 507, 1004, 1547, 1650, 1831
BstDSI	CCRYGG	1 cut(s)	617
BstEII	GGTNACC	1 cut(s)	563
BstF5I	GGATG	4 cut(s)	57, 1996, 2667, 2818
BstFNI	CGCG	2 cut(s)	675, 2982
BstH2I	RGCGCY	2 cut(s)	617, 1926
BstHHI	GCGC	5 cut(s)	616, 748, 1925, 2825, 2982
BstMAI	GTCTC	8 cut(s)	1190, 1380, 1902, 2182, 2273, 2463, 2553, 2849
BstNI	CCWGG	4 cut(s)	84, 408, 1097, 2786
BstNSI	RCATGY	2 cut(s)	1258, 2243
BstPI	GGTNACC	1 cut(s)	563
BstSCI	CCNGG	6 cut(s)	82, 321, 322, 406, 1095, 2784
BstSFI	CTRYAG	1 cut(s)	2894
BstSLI	GKGCMC	1 cut(s)	2852
BstUI	CGCG	2 cut(s)	675, 2982
BstV1I	GCAGC	7 cut(s)	612, 1068, 1113, 2134, 2252, 2401, 2941
BstV2I	GAAGAC	2 cut(s)	43, 1511
BstX2I	RGATCY	3 cut(s)	438, 1764, 2898
BstXI	CCANNNNNNTGG	2 cut(s)	2545, 2779
BstYI	RGATCY	3 cut(s)	438, 1764, 2898
Bsu15I	ATCGAT	2 cut(s)	2329, 2646
BsuRI	GGCC	9 cut(s)	190, 907, 999, 1549, 1713, 2386, 2424, 2537, 2875
BsuTUI	ATCGAT	2 cut(s)	2329, 2646
BtgI	CCRYGG	1 cut(s)	617
BtrI	CACGTC	1 cut(s)	2473
BtsCI	GGATG	4 cut(s)	57, 1996, 2667, 2818
BtsI	GCAGTG	1 cut(s)	2412
BtsIMutI	CAGTG	9 cut(s)	178, 900, 1188, 1292, 1755, 1843, 2281, 2412, 2779
Cac8I	GCNNGC	6 cut(s)	454, 507, 1004, 1547, 1650, 1831
CciI	TCATGA	1 cut(s)	2440
CfoI	GCGC	5 cut(s)	616, 748, 1925, 2825, 2982
Cfr13I	GGNCC	7 cut(s)	905, 1547, 1619, 1666, 2163, 2422, 2782
Cfr9I	CCCGGG	1 cut(s)	322
ClaI	ATCGAT	2 cut(s)	2329, 2646
CseI	GACGC	2 cut(s)	471, 2009
Csp6I	GTAC	4 cut(s)	399, 2117, 2130, 2305
CspCI	CAANNNNNGTGG	2 cut(s)	2720, 2755
CviQI	GTAC	4 cut(s)	399, 2117, 2130, 2305
DinI	GGCGCC	1 cut(s)	615
DraI	TTTAAA	1 cut(s)	963
DrdI	GACNNNNNNGTC	1 cut(s)	2789
DseDI	GACNNNNNNGTC	1 cut(s)	2789
EaeI	YGGCCR	3 cut(s)	997, 1711, 2535
Eam1104I	CTCTTC	5 cut(s)	1472, 1506, 1747, 2147, 2623
EarI	CTCTTC	5 cut(s)	1472, 1506, 1747, 2147, 2623
Ecl136II	GAGCTC	1 cut(s)	2229
Eco130I	CCWWGG	2 cut(s)	304, 617
Eco147I	AGGCCT	1 cut(s)	2875
Eco24I	GRGCYC	1 cut(s)	2231
Eco31I	GGTCTC	2 cut(s)	1902, 2553
Eco47I	GGWCC	4 cut(s)	1619, 1666, 2163, 2782
Eco47III	AGCGCT	1 cut(s)	1924
Eco53kI	GAGCTC	1 cut(s)	2229
Eco88I	CYCGRG	3 cut(s)	322, 1485, 2974
Eco91I	GGTNACC	1 cut(s)	563
EcoICRI	GAGCTC	1 cut(s)	2229
EcoO109I	RGGNCCY	1 cut(s)	2422
EcoO65I	GGTNACC	1 cut(s)	563
EcoRII	CCWGG	4 cut(s)	82, 406, 1095, 2784
EcoT14I	CCWWGG	2 cut(s)	304, 617
EcoT22I	ATGCAT	1 cut(s)	2439
EcoT38I	GRGCYC	1 cut(s)	2231
EgeI	GGCGCC	1 cut(s)	615
EheI	GGCGCC	1 cut(s)	615
ErhI	CCWWGG	2 cut(s)	304, 617
Esp3I	CGTCTC	1 cut(s)	2463
FalI	AAGNNNNNCTT	2 cut(s)	2190, 2222
FaqI	GGGAC	2 cut(s)	61, 533
FauI	CCCGC	4 cut(s)	388, 755, 1538, 1657
FauNDI	CATATG	1 cut(s)	1792
Fnu4HI	GCNGC	9 cut(s)	626, 1000, 1082, 1127, 2148, 2266, 2415, 2952, 2955
FokI	GGATG	4 cut(s)	44, 2003, 2654, 2825
FriOI	GRGCYC	1 cut(s)	2231
Fsp4HI	GCNGC	9 cut(s)	626, 1000, 1082, 1127, 2148, 2266, 2415, 2952, 2955
FspBI	CTAG	4 cut(s)	362, 1211, 1275, 2324
GlaI	GCGC	5 cut(s)	615, 747, 1924, 2824, 2981
GluI	GCNGC	9 cut(s)	626, 1000, 1082, 1127, 2148, 2266, 2415, 2952, 2955
GsaI	CCCAGC	1 cut(s)	554
GsuI	CTGGAG	4 cut(s)	521, 1044, 2592, 2696
HaeII	RGCGCY	2 cut(s)	617, 1926
HaeIII	GGCC	9 cut(s)	190, 907, 999, 1549, 1713, 2386, 2424, 2537, 2875
HapII	CCGG	8 cut(s)	207, 222, 323, 468, 479, 1119, 1617, 2625
HgaI	GACGC	2 cut(s)	471, 2009
HhaI	GCGC	5 cut(s)	616, 748, 1925, 2825, 2982
Hin1I	GRCGYC	3 cut(s)	482, 614, 2790
Hin6I	GCGC	5 cut(s)	614, 746, 1923, 2823, 2980
HinP1I	GCGC	5 cut(s)	614, 746, 1923, 2823, 2980
HincII	GTYRAC	2 cut(s)	942, 2067
HindII	GTYRAC	2 cut(s)	942, 2067
HindIII	AAGCTT	3 cut(s)	431, 1154, 1801
HpaI	GTTAAC	1 cut(s)	942
HpaII	CCGG	8 cut(s)	207, 222, 323, 468, 479, 1119, 1617, 2625
HphI	GGTGA	9 cut(s)	211, 241, 482, 631, 1117, 1349, 2032, 2147, 2720
Hpy166II	GTNNAC	6 cut(s)	244, 942, 1332, 2067, 2117, 2850
Hpy8I	GTNNAC	6 cut(s)	244, 942, 1332, 2067, 2117, 2850
HpyAV	CCTTC	9 cut(s)	89, 277, 911, 1535, 1981, 2699, 2750, 2881, 2886
HpyCH4III	ACNGT	4 cut(s)	182, 241, 1243, 2447
HpyCH4IV	ACGT	2 cut(s)	2472, 2790
HpySE526I	ACGT	2 cut(s)	2472, 2790
Hsp92I	GRCGYC	3 cut(s)	482, 614, 2790
HspAI	GCGC	5 cut(s)	614, 746, 1923, 2823, 2980
KasI	GGCGCC	1 cut(s)	613
Kpn2I	TCCGGA	1 cut(s)	1616
KspAI	GTTAAC	1 cut(s)	942
LguI	GCTCTTC	1 cut(s)	1506
LmnI	GCTCC	6 cut(s)	359, 502, 1195, 2411, 2611, 2962
Lsp1109I	GCAGC	7 cut(s)	612, 1068, 1113, 2134, 2252, 2401, 2941
LweI	GCATC	5 cut(s)	882, 1441, 2446, 2473, 2850
MaeI	CTAG	4 cut(s)	362, 1211, 1275, 2324
MaeII	ACGT	2 cut(s)	2472, 2790
MbiI	CCGCTC	1 cut(s)	762
MflI	RGATCY	3 cut(s)	438, 1764, 2898
MhlI	GDGCHC	4 cut(s)	2231, 2752, 2779, 2852
MlsI	TGGCCA	2 cut(s)	1713, 2537
MluNI	TGGCCA	2 cut(s)	1713, 2537
Mly113I	GGCGCC	1 cut(s)	614
MlyI	GAGTC	3 cut(s)	1525, 2492, 2829
MmeI	TCCRAC	2 cut(s)	597, 1644
Mox20I	TGGCCA	2 cut(s)	1713, 2537
Mph1103I	ATGCAT	1 cut(s)	2439
MroI	TCCGGA	1 cut(s)	1616
MroXI	GAANNNNTTC	2 cut(s)	533, 1075
MscI	TGGCCA	2 cut(s)	1713, 2537
MseI	TTAA	9 cut(s)	113, 435, 633, 941, 962, 1362, 1493, 1913, 1955
MslI	CAYNNNNRTG	2 cut(s)	2445, 2706
Msp20I	TGGCCA	2 cut(s)	1713, 2537
MspA1I	CMGCKG	3 cut(s)	554, 2579, 2954
MspCI	CTTAAG	2 cut(s)	434, 1912
MspI	CCGG	8 cut(s)	207, 222, 323, 468, 479, 1119, 1617, 2625
MspR9I	CCNGG	6 cut(s)	84, 323, 324, 408, 1097, 2786
Mva1269I	GAATGC	2 cut(s)	1075, 1294
MvaI	CCWGG	4 cut(s)	84, 408, 1097, 2786
MvnI	CGCG	2 cut(s)	675, 2982
NarI	GGCGCC	1 cut(s)	614
NciI	CCSGG	2 cut(s)	323, 324
NcoI	CCATGG	1 cut(s)	617
NdeI	CATATG	1 cut(s)	1792
NlaIV	GGNNCC	6 cut(s)	175, 615, 2590, 2607, 2697, 2744
NmeAIII	GCCGAG	1 cut(s)	2817
NmuCI	GTSAC	6 cut(s)	217, 275, 410, 470, 1356, 1578
NsiI	ATGCAT	1 cut(s)	2439
NspI	RCATGY	2 cut(s)	1258, 2243
NspV	TTCGAA	1 cut(s)	2908
PaeR7I	CTCGAG	1 cut(s)	2974
PagI	TCATGA	1 cut(s)	2440
PceI	AGGCCT	1 cut(s)	2875
PciI	ACATGT	1 cut(s)	1254
PciSI	GCTCTTC	1 cut(s)	1506
PctI	GAATGC	2 cut(s)	1075, 1294
PdmI	GAANNNNTTC	2 cut(s)	533, 1075
PfeI	GAWTC	9 cut(s)	229, 704, 841, 982, 1408, 1435, 1534, 1846, 2125
PflMI	CCANNNNNTGG	1 cut(s)	1067
PkrI	GCNGC	9 cut(s)	627, 1001, 1083, 1128, 2149, 2267, 2416, 2953, 2956
PleI	GAGTC	3 cut(s)	1524, 2492, 2829
PluTI	GGCGCC	1 cut(s)	617
PpsI	GAGTC	3 cut(s)	1524, 2492, 2829
PscI	ACATGT	1 cut(s)	1254
PshBI	ATTAAT	1 cut(s)	1955
Psp124BI	GAGCTC	1 cut(s)	2231
Psp6I	CCWGG	4 cut(s)	82, 406, 1095, 2784
PspEI	GGTNACC	1 cut(s)	563
PspFI	CCCAGC	1 cut(s)	550
PspGI	CCWGG	4 cut(s)	82, 406, 1095, 2784
PspN4I	GGNNCC	6 cut(s)	175, 615, 2590, 2607, 2697, 2744
PspPI	GGNCC	7 cut(s)	905, 1547, 1619, 1666, 2163, 2422, 2782
PspXI	VCTCGAGB	1 cut(s)	2974
PstI	CTGCAG	1 cut(s)	2898
PsuI	RGATCY	3 cut(s)	438, 1764, 2898
PvuII	CAGCTG	2 cut(s)	554, 2579
RsaI	GTAC	4 cut(s)	400, 2118, 2131, 2306
RsaNI	GTAC	4 cut(s)	399, 2117, 2130, 2305
RseI	CAYNNNNRTG	2 cut(s)	2445, 2706
SacI	GAGCTC	1 cut(s)	2231
SapI	GCTCTTC	1 cut(s)	1506
SaqAI	TTAA	9 cut(s)	113, 435, 633, 941, 962, 1362, 1493, 1913, 1955
SatI	GCNGC	9 cut(s)	626, 1000, 1082, 1127, 2148, 2266, 2415, 2952, 2955
Sau96I	GGNCC	7 cut(s)	905, 1547, 1619, 1666, 2163, 2422, 2782
ScaI	AGTACT	1 cut(s)	2306
SchI	GAGTC	3 cut(s)	1525, 2492, 2829
ScrFI	CCNGG	6 cut(s)	84, 323, 324, 408, 1097, 2786
SduI	GDGCHC	4 cut(s)	2231, 2752, 2779, 2852
SfaNI	GCATC	5 cut(s)	882, 1441, 2446, 2473, 2850
SfcI	CTRYAG	1 cut(s)	2894
SfoI	GGCGCC	1 cut(s)	615
Sfr274I	CTCGAG	1 cut(s)	2974
SfuI	TTCGAA	1 cut(s)	2908
SinI	GGWCC	4 cut(s)	1619, 1666, 2163, 2782
SlaI	CTCGAG	1 cut(s)	2974
SmaI	CCCGGG	1 cut(s)	324
SmiMI	CAYNNNNRTG	2 cut(s)	2445, 2706
SseBI	AGGCCT	1 cut(s)	2875
SsiI	CCGC	7 cut(s)	395, 762, 777, 1000, 1545, 1650, 2952
SspDI	GGCGCC	1 cut(s)	613
SspI	AATATT	1 cut(s)	735
SspMI	CTAG	4 cut(s)	362, 1211, 1275, 2324
SstI	GAGCTC	1 cut(s)	2231
StuI	AGGCCT	1 cut(s)	2875
StyD4I	CCNGG	6 cut(s)	82, 321, 322, 406, 1095, 2784
StyI	CCWWGG	2 cut(s)	304, 617
TaaI	ACNGT	4 cut(s)	182, 241, 1243, 2447
TaiI	ACGT	2 cut(s)	2475, 2793
TaqI	TCGA	9 cut(s)	417, 1086, 2236, 2317, 2329, 2467, 2646, 2908, 2975
TaqII	GACCGA	1 cut(s)	1884
TatI	WGTACW	1 cut(s)	2304
TauI	GCSGC	2 cut(s)	1002, 2954
TfiI	GAWTC	9 cut(s)	229, 704, 841, 982, 1408, 1435, 1534, 1846, 2125
Tru1I	TTAA	9 cut(s)	113, 435, 633, 941, 962, 1362, 1493, 1913, 1955
Tru9I	TTAA	9 cut(s)	113, 435, 633, 941, 962, 1362, 1493, 1913, 1955
TscAI	CASTG	9 cut(s)	185, 907, 1195, 1299, 1762, 1843, 2281, 2412, 2779
TseFI	GTSAC	6 cut(s)	217, 275, 410, 470, 1356, 1578
TseI	GCWGC	7 cut(s)	625, 1081, 1126, 2147, 2265, 2414, 2954
Tsp45I	GTSAC	6 cut(s)	217, 275, 410, 470, 1356, 1578
TspGWI	ACGGA	2 cut(s)	205, 2571
TspMI	CCCGGG	1 cut(s)	322
TspRI	CASTG	9 cut(s)	185, 907, 1195, 1299, 1762, 1843, 2281, 2412, 2779
Van91I	CCANNNNNTGG	1 cut(s)	1067
Vha464I	CTTAAG	2 cut(s)	434, 1912
VneI	GTGCAC	1 cut(s)	2848
VpaK11BI	GGWCC	4 cut(s)	1619, 1666, 2163, 2782
VspI	ATTAAT	1 cut(s)	1955
XapI	RAATTY	5 cut(s)	682, 796, 1297, 1695, 2035
XceI	RCATGY	2 cut(s)	1258, 2243
XhoI	CTCGAG	1 cut(s)	2974
XmaI	CCCGGG	1 cut(s)	322
XmnI	GAANNNNTTC	2 cut(s)	533, 1075
XspI	CTAG	4 cut(s)	362, 1211, 1275, 2324
ZraI	GACGTC	1 cut(s)	2791
ZrmI	AGTACT	1 cut(s)	2306
Zsp2I	ATGCAT	1 cut(s)	2439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.