MD02G1066300.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001321
Physical Location & Seq
Forward (+)
5399896 .. 5403400
3505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1066300.v1.1.491

Sequence Viewer

Length: 3048 bp
ATGGCTAAACACACCCCAACATCTTCTCTCCAAACCCACCTCCACCTCCTCCTCCTTCTCCTCCTCCTCCTCCTCCTCCGCCATGCCAACTCCCAATCCCTCCAAGACCAAGAACAAGCAGTCCTTCTGAAACTCAAGTCCTACTTAGATTCTCCACCATCTCTCAGCCATTGGATCCCATCAAACTCAAACACTTCTTCCCACTGCTCCTGGCCTGAAATTTCCTGCACAAACAACTCCGTCACTGGATTGCTTCTCGTCAACTTGAACATAACCCTACCGGTTCCACCCTTCATCTGTGATCTCACAAACCTCACACTCGTCGATCTCAACACCAACTATCTCACCGGAGAATTCCCAAAATCTCTCTACAAGTGTTCCAAACTAGAGTACTTAGACCTCTCACAAAACTACTTTGTAGGCCCGATTCCTGATGACATCGACAGCCTGCCCCGGCTTAAGCAGCTTATCCTCGCCGGAAACAACTTCTCCAACATTCCTCCTGCGATAGGGCGGTTGCAAGAGCTCGAGAATCTTCAGCTGTGGATGAACCAGTTCAATGGCTCTGTCCCACCAGAAATAGGTAACTTGTCCAATCTCAAAGACCTAAACATGTCTTGGAACACAAAACTTGTGCCTTGGACCTTGCCTTCCAATTTCACCAAGTTGAAAAAGCTCAGTAATTTATGGATACGCCAATCGAACTTGATTGGAGAGCTTCCTGAGACACTTGGGGAGATGGAAGCATTGGAAGTAATTGATTTGGCAATAAACACTTTGAGTGGGAGGATCCCGAACAGTTTGTTTACGTTGAAGAATTTGAGTATAGTCTATCTCTTCAAGAACACGCTTTCCGGGGAGGTTCCTCAAGTGATTGAGTCATGGAACTTGAGCATTCTTGATATCTCTGAGAACACTTTATCAGGGACAATACCAGAAGAGTACGGCAATCTTACCAAATTAACTCAGTTGGCTTTGTTTCTCAATGATTTTTCGGGTGAAATTCCCAAGAGCATTGGCCGCCTGCCAAACCTCAGAGACTTCAAAATCTTCAACAACAATTTTTCAGGAACATTGCCTCCAGAGCTTGGGAAGTACTCAAAGCTCGAAGCTTTTCAAGTTTGCGTCAATAGGCTAACAGGAAAACTGCCAGATGATTTGTGCTATTGGGGTAAGCTGGACGATGTTATAGCTTATGGGAATCATCTGAGTGGAGAATTGCCAAGCTCTCTTGGAAATTGCAGCAGTTTGAAGACTGTCAAGGTTCAAGATAACATGCTGTCTGGAAACATTCCTAGCGGTATGTGGACGGCTCCAAACTTGACTACTGTGCTGGTGAGCAACAACTCGCTTACTGGCAAGCTTCCTGAGAAGATGTCATCAGATCTTTCGCGGTTGGAAATGCGAGACAACAAATTTTCGGGCAACATTCCAACCGGGGTGTCTTCCTGGACAGGTTTGAAGGTTTTTGACGCCGGTAATAACCACTTAAGCGGTGCCGTTCCTCGGGAATTAACCGCGCTTTCTAGCTTAATGACTCTTTCTCTGGATCAGAATCAGCTCACCGGCTCCCTTCCATCGGATATTGTGTCGTGGGAGTCACTCGCTAGTCTTAATTTCAGTCGAAATCAGCTCTCTGGAACAATTCCTGAGAAGCTTGGTCTTCTGCCGGGACTTACTGGATTAGACCTTTCAGCAAACCAACTTTCTGGCGAAATTCCAGACCAACTCGGGCGTCTGAAGCTCAACCAATTCAATCTCTCTTCCAATCAACTCTCCGGGGATATCCCTATCGAATTTGAAAACGCTGCGTATGAAGGAAGCTTCTTGGACAATCGAGGCCTCTGCGCACCTAGCTCAGCGGCAATAAAGCTCCCCATATGCAATTCTGAATTCCGAAAGTCCAGTAAACTTTCGTCGAAATCTCTTGCACTGATCTTATCTTTAGGCATACTATTGTGCTTGTTGGCTTTTTCCATCACGGTCATCGTGGTCAGAAGTTACTGGAAGAGAAATGGTGATTCTAACTGGAAGCTCAACTCATTTCAGAGGTTGAATTTCTCAGTGTCGAAAATCCTATCGGGGTTGACTGAACGTAATCTGATTGGAAGTGGTGGATCAGGAAAAGTTTATCGCGTTCCTGTGAATCGCGTTGGTGGTGTTGTTGCTGTGAAGAAGATTTGGAAGAATAAGAATTTAGAAGAGAAGCTTGAGAAAGAGTTTCTTGCAGAAGTCAAGATCTTGAGCTCAATTCGACACGCCAACATAGTGAAGCTGATGTGCTGCATTTCCAGTAAGACTTCAAAACTTCTCGTCTACGAGTACTTAGATAATCGGAGCCTGGATCGATGGCTGCACAGGAGAAACAGGCCATCCAATCTCTCGGGGTCAGTCCAACATGTCGCGCTCGACTGGCCTAAGAGGTTGCAGATTGCAGTGGGGGCAGCTCAGGGCCTCAGCTATATGCACCACGACTGCGTGCCGCCTGTGGTGCATCGTGACGTGAAATCGAGCAACATTTTGTTGGACTCCGATTTCAATGCAAAAATAGCAGACTTTGGTCTGGCTAAGATGTTGGTCAAGCAAGGAGAACTTGCTACAATGTCATCTGTTGCTGGCTCCTTTGGCTACATGGCTCCAGAATACGCTCACACAACGCGAGTGAATGAAAAGATCGATGTGTATAGTTTTGGGGTCATCCTTCTGGAGTTGACAACCGGCAGGGAAGCCCATGACGGCGATGAACACACTGCCCTTGCTGAATGGGCGTGGCGCCATGTTCACGAAGAAAATGCTATCCCTGATGCTTTGGACCAGGACGTCAAAGAACCTTGTTACTTGGACGAAATGTGCTCGGTTTTCAAACTTGGCCTCAGTTGCACAGAGAGACTTCCAGCTAGTAGGCCTTCCATGAAGGTCGTTCTGCAAATCTTGCTCCGATGCAGCCGTCCAGTTATCAACGGTGAAAAGACCGAGTACTTTGCCACTCCTCTGCTCAAGAACTCGAAGCGCGAGAGGATTTTGGAAGACGGGAATGGTCGGTCTTAA

Protein Analysis

1016

Amino Acids

112.35

Weight (kDa)

7.25

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 77 6.6e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 104 - 163 5.2e-09 Leucine rich repeat
LRR_14 PF23598 144 - 372 7e-19 Leucine-rich repeat region
LRR_14 PF23598 267 - 466 5.6e-07 Leucine-rich repeat region
LRR_8 PF13855 510 - 569 4.5e-06 Leucine rich repeat
Pkinase PF00069 699 - 976 1.7e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 699 - 977 1.1e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2819
AatII GACGTC 1 cut(s) 2823
Acc16I TGCGCA 1 cut(s) 1849
AccB1I GGYRCC 2 cut(s) 1496, 2772
AccB7I CCANNNNNTGG 1 cut(s) 1088
AccI GTMKAC 1 cut(s) 2316
AccII CGCG 7 cut(s) 1393, 1520, 2136, 2151, 2405, 2659, 3012
AciI CCGC 9 cut(s) 79, 514, 1021, 1299, 1393, 1494, 1518, 1862, 2483
AclWI GGATC 7 cut(s) 169, 182, 784, 797, 1557, 2125, 2352
AcoI YGGCCR 1 cut(s) 1018
AcuI CTGAAG 2 cut(s) 521, 1760
AcyI GRCGYC 4 cut(s) 1473, 1735, 2773, 2820
AfaI GTAC 5 cut(s) 392, 944, 1097, 2324, 2978
AfiI CCNNNNNNNGG 5 cut(s) 509, 581, 1088, 1506, 1579
AflII CTTAAG 2 cut(s) 458, 1489
AflIII ACRYGT 2 cut(s) 612, 2398
AgeI ACCGGT 1 cut(s) 280
AjiI CACGTC 1 cut(s) 2503
AjnI CCWGG 4 cut(s) 209, 1448, 2340, 2814
AloI GAACNNNNNNTCC 6 cut(s) 105, 137, 836, 868, 1063, 1095
Alw21I GWGCWC 3 cut(s) 528, 2249, 2855
Alw26I GTCTC 4 cut(s) 719, 1032, 1401, 2881
AlwI GGATC 7 cut(s) 169, 182, 784, 797, 1557, 2125, 2352
Ama87I CYCGRG 4 cut(s) 527, 1506, 1730, 2383
AoxI GGCC 9 cut(s) 212, 421, 1018, 1840, 2369, 2414, 2452, 2869, 2903
ApeKI GCWGC 7 cut(s) 463, 1242, 1808, 2283, 2353, 2444, 2943
AsiGI ACCGGT 1 cut(s) 280
Asp700I GAANNNNTTC 2 cut(s) 554, 1113
AspLEI GCGC 5 cut(s) 1522, 1850, 2407, 2775, 3012
AspS9I GGNCC 4 cut(s) 422, 642, 2452, 2812
AsuC2I CCSGG 5 cut(s) 454, 856, 1438, 1671, 1780
AsuHPI GGTGA 7 cut(s) 337, 652, 1010, 1348, 1555, 2030, 2975
AvaI CYCGRG 4 cut(s) 527, 1506, 1730, 2383
AvaII GGWCC 2 cut(s) 642, 2812
BamHI GGATCC 2 cut(s) 174, 789
BanI GGYRCC 2 cut(s) 1496, 2772
BanII GRGCYC 2 cut(s) 528, 2249
BbsI GAAGAC 4 cut(s) 1259, 1437, 1655, 3033
Bbv12I GWGCWC 3 cut(s) 528, 2249, 2855
BbvCI CCTCAGC 1 cut(s) 2456
BbvI GCAGC 7 cut(s) 475, 1254, 1795, 2270, 2340, 2456, 2955
BccI CCATC 7 cut(s) 166, 187, 733, 1585, 1985, 2343, 2380
BceAI ACGGC 5 cut(s) 961, 1326, 1484, 2752, 2931
BcgI CGANNNNNNTGC 8 cut(s) 1827, 1861, 2522, 2556, 2774, 2808, 2963, 2997
BciT130I CCWGG 4 cut(s) 211, 1450, 2342, 2816
BciVI GTATCC 1 cut(s) 684
BcnI CCSGG 5 cut(s) 454, 856, 1438, 1671, 1780
BcoDI GTCTC 4 cut(s) 719, 1032, 1401, 2881
BfaI CTAG 6 cut(s) 386, 1296, 1527, 1608, 1854, 2898
BfoI RGCGCY 1 cut(s) 2776
BfrI CTTAAG 2 cut(s) 458, 1489
BfuI GTATCC 1 cut(s) 684
BglII AGATCT 2 cut(s) 1384, 2238
BlpI GCTNAGC 1 cut(s) 1858
BmcAI AGTACT 4 cut(s) 392, 1097, 2324, 2978
Bme1390I CCNGG 9 cut(s) 211, 454, 856, 1438, 1450, 1671, 1780, 2342, 2816
Bme18I GGWCC 2 cut(s) 642, 2812
BmeT110I CYCGRG 4 cut(s) 527, 1506, 1730, 2383
BmgBI CACGTC 1 cut(s) 2503
BmgT120I GGNCC 4 cut(s) 422, 642, 2452, 2812
BmrFI CCNGG 9 cut(s) 211, 454, 856, 1438, 1450, 1671, 1780, 2342, 2816
BmsI GCATC 3 cut(s) 2503, 2794, 2930
BoxI GACNNNNGTC 1 cut(s) 2559
BpiI GAAGAC 4 cut(s) 1259, 1437, 1655, 3033
BpmI CTGGAG 3 cut(s) 1065, 2622, 2726
Bpu10I CCTNAGC 2 cut(s) 2448, 2456
Bpu1102I GCTNAGC 1 cut(s) 1858
BpuEI CTTGAG 6 cut(s) 119, 852, 910, 2231, 2263, 2981
BpuMI CCSGG 5 cut(s) 454, 856, 1438, 1671, 1780
Bsa29I ATCGAT 2 cut(s) 2347, 2676
BsaBI GATNNNNATC 1 cut(s) 1554
BsaHI GRCGYC 4 cut(s) 1473, 1735, 2773, 2820
BsaJI CCNNGG 6 cut(s) 452, 638, 855, 1437, 1505, 1779
BsaWI WCCGGW 2 cut(s) 280, 347
BsaXI ACNNNNNCTCC 4 cut(s) 473, 503, 1063, 1093
Bsc4I CCNNNNNNNGG 5 cut(s) 509, 581, 1088, 1506, 1579
Bse118I RCCGGY 4 cut(s) 280, 1475, 1565, 2717
Bse3DI GCAATG 1 cut(s) 1073
Bse8I GATNNNNATC 1 cut(s) 1554
BseBI CCWGG 4 cut(s) 211, 1450, 2342, 2816
BseCI ATCGAT 2 cut(s) 2347, 2676
BseDI CCNNGG 6 cut(s) 452, 638, 855, 1437, 1505, 1779
BseGI GGATG 3 cut(s) 552, 2372, 2697
BseJI GATNNNNATC 1 cut(s) 1554
BseLI CCNNNNNNNGG 5 cut(s) 509, 581, 1088, 1506, 1579
BseMI GCAATG 1 cut(s) 1073
BseRI GAGGAG 9 cut(s) 38, 41, 50, 53, 56, 59, 62, 65, 2979
BseXI GCAGC 7 cut(s) 475, 1254, 1795, 2270, 2340, 2456, 2955
BsgI GTGCAG 2 cut(s) 211, 2339
Bsh1236I CGCG 7 cut(s) 1393, 1520, 2136, 2151, 2405, 2659, 3012
BshFI GGCC 9 cut(s) 214, 423, 1020, 1842, 2371, 2416, 2454, 2871, 2905
BshNI GGYRCC 2 cut(s) 1496, 2772
BshTI ACCGGT 1 cut(s) 280
BshVI ATCGAT 2 cut(s) 2347, 2676
BsiHKAI GWGCWC 3 cut(s) 528, 2249, 2855
BsiHKCI CYCGRG 4 cut(s) 527, 1506, 1730, 2383
BslFI GGGAC 3 cut(s) 554, 940, 1686
BslI CCNNNNNNNGG 5 cut(s) 509, 581, 1088, 1506, 1579
BsmAI GTCTC 4 cut(s) 719, 1032, 1401, 2881
BsmFI GGGAC 3 cut(s) 554, 940, 1686
BsmI GAATGC 1 cut(s) 894
BsnI GGCC 9 cut(s) 214, 423, 1020, 1842, 2371, 2416, 2454, 2871, 2905
BsoBI CYCGRG 4 cut(s) 527, 1506, 1730, 2383
Bsp1286I GDGCHC 3 cut(s) 528, 2249, 2855
Bsp1720I GCTNAGC 1 cut(s) 1858
BspACI CCGC 9 cut(s) 79, 514, 1021, 1299, 1393, 1494, 1518, 1862, 2483
BspANI GGCC 9 cut(s) 214, 423, 1020, 1842, 2371, 2416, 2454, 2871, 2905
BspDI ATCGAT 2 cut(s) 2347, 2676
BspFNI CGCG 7 cut(s) 1393, 1520, 2136, 2151, 2405, 2659, 3012
BspPI GGATC 7 cut(s) 169, 182, 784, 797, 1557, 2125, 2352
BspT107I GGYRCC 2 cut(s) 1496, 2772
BspTI CTTAAG 2 cut(s) 458, 1489
BsrDI GCAATG 1 cut(s) 1073
BsrFI RCCGGY 4 cut(s) 280, 1475, 1565, 2717
BssAI RCCGGY 4 cut(s) 280, 1475, 1565, 2717
BssECI CCNNGG 6 cut(s) 452, 638, 855, 1437, 1505, 1779
BssNI GRCGYC 4 cut(s) 1473, 1735, 2773, 2820
BssT1I CCWWGG 1 cut(s) 638
Bst2UI CCWGG 4 cut(s) 211, 1450, 2342, 2816
Bst4CI ACNGT 5 cut(s) 800, 1258, 1330, 1984, 2963
Bst6I CTCTTC 5 cut(s) 842, 933, 1768, 2003, 2196
BstACI GRCGYC 4 cut(s) 1473, 1735, 2773, 2820
BstAFI CTTAAG 2 cut(s) 458, 1489
BstAPI GCANNNNNTGC 1 cut(s) 2932
BstC8I GCNNGC 5 cut(s) 449, 1025, 1361, 2480, 2617
BstENI CCTNNNNNAGG 1 cut(s) 507
BstF5I GGATG 3 cut(s) 552, 2372, 2697
BstFNI CGCG 7 cut(s) 1393, 1520, 2136, 2151, 2405, 2659, 3012
BstH2I RGCGCY 1 cut(s) 2776
BstHHI GCGC 5 cut(s) 1522, 1850, 2407, 2775, 3012
BstMAI GTCTC 4 cut(s) 719, 1032, 1401, 2881
BstNI CCWGG 4 cut(s) 211, 1450, 2342, 2816
BstNSI RCATGY 3 cut(s) 616, 1279, 2402
BstPAI GACNNNNGTC 1 cut(s) 2559
BstSCI CCNGG 9 cut(s) 209, 452, 854, 1436, 1448, 1669, 1778, 2340, 2814
BstUI CGCG 7 cut(s) 1393, 1520, 2136, 2151, 2405, 2659, 3012
BstV1I GCAGC 7 cut(s) 475, 1254, 1795, 2270, 2340, 2456, 2955
BstV2I GAAGAC 4 cut(s) 1259, 1437, 1655, 3033
BstX2I RGATCY 4 cut(s) 174, 789, 1384, 2238
BstXI CCANNNNNNTGG 2 cut(s) 560, 1709
BstYI RGATCY 4 cut(s) 174, 789, 1384, 2238
Bsu15I ATCGAT 2 cut(s) 2347, 2676
BsuI GTATCC 1 cut(s) 684
BsuRI GGCC 9 cut(s) 214, 423, 1020, 1842, 2371, 2416, 2454, 2871, 2905
BsuTUI ATCGAT 2 cut(s) 2347, 2676
BtgZI GCGATG 1 cut(s) 2754
BtrI CACGTC 1 cut(s) 2503
BtsCI GGATG 3 cut(s) 552, 2372, 2697
BtsI GCAGTG 3 cut(s) 202, 2442, 2748
BtsIMutI CAGTG 6 cut(s) 202, 243, 1931, 2070, 2442, 2748
Cac8I GCNNGC 5 cut(s) 449, 1025, 1361, 2480, 2617
CfoI GCGC 5 cut(s) 1522, 1850, 2407, 2775, 3012
Cfr10I RCCGGY 4 cut(s) 280, 1475, 1565, 2717
Cfr13I GGNCC 4 cut(s) 422, 642, 2452, 2812
ClaI ATCGAT 2 cut(s) 2347, 2676
CseI GACGC 3 cut(s) 1114, 1481, 1724
Csp6I GTAC 5 cut(s) 391, 943, 1096, 2323, 2977
CspAI ACCGGT 1 cut(s) 280
CviAII CATG 9 cut(s) 83, 613, 882, 1276, 2399, 2632, 2732, 2777, 2911
CviQI GTAC 5 cut(s) 391, 943, 1096, 2323, 2977
DinI GGCGCC 1 cut(s) 2774
DrdI GACNNNNNNGTC 1 cut(s) 2819
DseDI GACNNNNNNGTC 1 cut(s) 2819
EaeI YGGCCR 1 cut(s) 1018
Eam1104I CTCTTC 5 cut(s) 842, 933, 1768, 2003, 2196
EarI CTCTTC 5 cut(s) 842, 933, 1768, 2003, 2196
EciI GGCGGA 1 cut(s) 68
Ecl136II GAGCTC 2 cut(s) 526, 2247
Eco130I CCWWGG 1 cut(s) 638
Eco147I AGGCCT 2 cut(s) 1842, 2905
Eco24I GRGCYC 2 cut(s) 528, 2249
Eco32I GATATC 2 cut(s) 904, 1786
Eco47I GGWCC 2 cut(s) 642, 2812
Eco53kI GAGCTC 2 cut(s) 526, 2247
Eco57I CTGAAG 2 cut(s) 521, 1760
Eco88I CYCGRG 4 cut(s) 527, 1506, 1730, 2383
EcoICRI GAGCTC 2 cut(s) 526, 2247
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoO109I RGGNCCY 1 cut(s) 2452
EcoRI GAATTC 2 cut(s) 353, 1892
EcoRII CCWGG 4 cut(s) 209, 1448, 2340, 2814
EcoRV GATATC 2 cut(s) 904, 1786
EcoT14I CCWWGG 1 cut(s) 638
EcoT38I GRGCYC 2 cut(s) 528, 2249
EgeI GGCGCC 1 cut(s) 2774
EheI GGCGCC 1 cut(s) 2774
ErhI CCWWGG 1 cut(s) 638
FaeI CATG 9 cut(s) 86, 616, 885, 1279, 2402, 2635, 2735, 2780, 2914
FaqI GGGAC 3 cut(s) 554, 940, 1686
FatI CATG 9 cut(s) 82, 612, 881, 1275, 2398, 2631, 2731, 2776, 2910
FauNDI CATATG 1 cut(s) 1880
FblI GTMKAC 1 cut(s) 2316
FokI GGATG 3 cut(s) 559, 2359, 2684
FriOI GRGCYC 2 cut(s) 528, 2249
FspBI CTAG 6 cut(s) 386, 1296, 1527, 1608, 1854, 2898
FspI TGCGCA 1 cut(s) 1849
GlaI GCGC 5 cut(s) 1521, 1849, 2406, 2774, 3011
GsuI CTGGAG 3 cut(s) 1065, 2622, 2726
HaeII RGCGCY 1 cut(s) 2776
HaeIII GGCC 9 cut(s) 214, 423, 1020, 1842, 2371, 2416, 2454, 2871, 2905
HgaI GACGC 3 cut(s) 1114, 1481, 1724
HhaI GCGC 5 cut(s) 1522, 1850, 2407, 2775, 3012
Hin1I GRCGYC 4 cut(s) 1473, 1735, 2773, 2820
Hin1II CATG 9 cut(s) 86, 616, 885, 1279, 2402, 2635, 2735, 2780, 2914
Hin6I GCGC 5 cut(s) 1520, 1848, 2405, 2773, 3010
HinP1I GCGC 5 cut(s) 1520, 1848, 2405, 2773, 3010
HincII GTYRAC 3 cut(s) 262, 2088, 2712
HindII GTYRAC 3 cut(s) 262, 2088, 2712
HindIII AAGCTT 5 cut(s) 1110, 1361, 1655, 1822, 2207
HphI GGTGA 7 cut(s) 337, 652, 1010, 1348, 1555, 2030, 2975
Hpy166II GTNNAC 8 cut(s) 262, 807, 1308, 1910, 2088, 2317, 2712, 2782
Hpy8I GTNNAC 8 cut(s) 262, 807, 1308, 1910, 2088, 2317, 2712, 2782
Hpy99I CGWCG 2 cut(s) 326, 1921
HpyCH4III ACNGT 5 cut(s) 800, 1258, 1330, 1984, 2963
HpyCH4IV ACGT 4 cut(s) 809, 2095, 2502, 2820
HpySE526I ACGT 4 cut(s) 809, 2095, 2502, 2820
Hsp92I GRCGYC 4 cut(s) 1473, 1735, 2773, 2820
Hsp92II CATG 9 cut(s) 86, 616, 885, 1279, 2402, 2635, 2735, 2780, 2914
HspAI GCGC 5 cut(s) 1520, 1848, 2405, 2773, 3010
KasI GGCGCC 1 cut(s) 2772
LmnI GCTCC 8 cut(s) 212, 1318, 1574, 1878, 2337, 2624, 2641, 2940
Lsp1109I GCAGC 7 cut(s) 475, 1254, 1795, 2270, 2340, 2456, 2955
LweI GCATC 3 cut(s) 2503, 2794, 2930
MaeI CTAG 6 cut(s) 386, 1296, 1527, 1608, 1854, 2898
MaeII ACGT 4 cut(s) 809, 2095, 2502, 2820
MaeIII GTNAC 6 cut(s) 241, 584, 1599, 2000, 2498, 2834
MflI RGATCY 4 cut(s) 174, 789, 1384, 2238
MhlI GDGCHC 3 cut(s) 528, 2249, 2855
Mly113I GGCGCC 1 cut(s) 2773
MlyI GAGTC 4 cut(s) 887, 1531, 1607, 2522
MmeI TCCRAC 5 cut(s) 516, 1377, 1457, 2419, 2505
MroXI GAANNNNTTC 2 cut(s) 554, 1113
MseI TTAA 7 cut(s) 459, 962, 1490, 1514, 1532, 1614, 3046
MslI CAYNNNNRTG 1 cut(s) 1209
MspA1I CMGCKG 2 cut(s) 541, 1862
MspCI CTTAAG 2 cut(s) 458, 1489
MspR9I CCNGG 9 cut(s) 211, 454, 856, 1438, 1450, 1671, 1780, 2342, 2816
Mva1269I GAATGC 1 cut(s) 894
MvaI CCWGG 4 cut(s) 211, 1450, 2342, 2816
MvnI CGCG 7 cut(s) 1393, 1520, 2136, 2151, 2405, 2659, 3012
NarI GGCGCC 1 cut(s) 2773
NciI CCSGG 5 cut(s) 454, 856, 1438, 1671, 1780
NdeI CATATG 1 cut(s) 1880
NlaIII CATG 9 cut(s) 86, 616, 885, 1279, 2402, 2635, 2735, 2780, 2914
NmuCI GTSAC 3 cut(s) 241, 1599, 2498
NsbI TGCGCA 1 cut(s) 1849
NspI RCATGY 3 cut(s) 616, 1279, 2402
PaeR7I CTCGAG 1 cut(s) 527
PceI AGGCCT 2 cut(s) 1842, 2905
PciI ACATGT 2 cut(s) 612, 2398
PctI GAATGC 1 cut(s) 894
PdmI GAANNNNTTC 2 cut(s) 554, 1113
PfeI GAWTC 7 cut(s) 149, 427, 532, 1201, 1555, 2021, 2146
PflMI CCANNNNNTGG 1 cut(s) 1088
PfoI TCCNGGA 1 cut(s) 1448
PinAI ACCGGT 1 cut(s) 280
PleI GAGTC 4 cut(s) 886, 1531, 1606, 2522
PluTI GGCGCC 1 cut(s) 2776
PpsI GAGTC 4 cut(s) 886, 1531, 1606, 2522
PscI ACATGT 2 cut(s) 612, 2398
PshAI GACNNNNGTC 1 cut(s) 2559
Psp124BI GAGCTC 2 cut(s) 528, 2249
Psp6I CCWGG 4 cut(s) 209, 1448, 2340, 2814
PspGI CCWGG 4 cut(s) 209, 1448, 2340, 2814
PspPI GGNCC 4 cut(s) 422, 642, 2452, 2812
PsuI RGATCY 4 cut(s) 174, 789, 1384, 2238
PvuII CAGCTG 1 cut(s) 541
RsaI GTAC 5 cut(s) 392, 944, 1097, 2324, 2978
RsaNI GTAC 5 cut(s) 391, 943, 1096, 2323, 2977
RseI CAYNNNNRTG 1 cut(s) 1209
SacI GAGCTC 2 cut(s) 528, 2249
SaqAI TTAA 7 cut(s) 459, 962, 1490, 1514, 1532, 1614, 3046
Sau96I GGNCC 4 cut(s) 422, 642, 2452, 2812
ScaI AGTACT 4 cut(s) 392, 1097, 2324, 2978
SchI GAGTC 4 cut(s) 887, 1531, 1607, 2522
ScrFI CCNGG 9 cut(s) 211, 454, 856, 1438, 1450, 1671, 1780, 2342, 2816
SduI GDGCHC 3 cut(s) 528, 2249, 2855
SfaNI GCATC 3 cut(s) 2503, 2794, 2930
SfoI GGCGCC 1 cut(s) 2774
Sfr274I CTCGAG 1 cut(s) 527
SinI GGWCC 2 cut(s) 642, 2812
SlaI CTCGAG 1 cut(s) 527
SmiMI CAYNNNNRTG 1 cut(s) 1209
SmlI CTYRAG 9 cut(s) 134, 458, 527, 867, 889, 1489, 2210, 2242, 2996
SmoI CTYRAG 9 cut(s) 134, 458, 527, 867, 889, 1489, 2210, 2242, 2996
SseBI AGGCCT 2 cut(s) 1842, 2905
SsiI CCGC 9 cut(s) 79, 514, 1021, 1299, 1393, 1494, 1518, 1862, 2483
SspDI GGCGCC 1 cut(s) 2772
SspMI CTAG 6 cut(s) 386, 1296, 1527, 1608, 1854, 2898
SstI GAGCTC 2 cut(s) 528, 2249
StuI AGGCCT 2 cut(s) 1842, 2905
StyD4I CCNGG 9 cut(s) 209, 452, 854, 1436, 1448, 1669, 1778, 2340, 2814
StyI CCWWGG 1 cut(s) 638
TaaI ACNGT 5 cut(s) 800, 1258, 1330, 1984, 2963
TaiI ACGT 4 cut(s) 812, 2098, 2505, 2823
TaqII GACCGA 2 cut(s) 2987, 3030
TatI WGTACW 4 cut(s) 390, 1095, 2322, 2976
TauI GCSGC 3 cut(s) 1023, 1865, 2485
TfiI GAWTC 7 cut(s) 149, 427, 532, 1201, 1555, 2021, 2146
Tru1I TTAA 7 cut(s) 459, 962, 1490, 1514, 1532, 1614, 3046
Tru9I TTAA 7 cut(s) 459, 962, 1490, 1514, 1532, 1614, 3046
TscAI CASTG 6 cut(s) 209, 250, 1938, 2070, 2442, 2755
TseFI GTSAC 3 cut(s) 241, 1599, 2498
TseI GCWGC 7 cut(s) 463, 1242, 1808, 2283, 2353, 2444, 2943
Tsp45I GTSAC 3 cut(s) 241, 1599, 2498
TspDTI ATGAA 6 cut(s) 283, 563, 1830, 2682, 2757, 2927
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 6 cut(s) 209, 250, 1938, 2070, 2442, 2755
Van91I CCANNNNNTGG 1 cut(s) 1088
Vha464I CTTAAG 2 cut(s) 458, 1489
VpaK11BI GGWCC 2 cut(s) 642, 2812
XagI CCTNNNNNAGG 1 cut(s) 507
XceI RCATGY 3 cut(s) 616, 1279, 2402
XhoI CTCGAG 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 2316
XmnI GAANNNNTTC 2 cut(s) 554, 1113
XspI CTAG 6 cut(s) 386, 1296, 1527, 1608, 1854, 2898
ZraI GACGTC 1 cut(s) 2821
ZrmI AGTACT 4 cut(s) 392, 1097, 2324, 2978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.