Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0001321

Physical Location & Seq

Genomic Coordinates Reverse (-)

87129951 .. 87133523

Gene Structure 3573 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00149580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3042 bp

ATGACTACACCAACCCCAAAACCCTCTCTCATCTTCCTCATCTTCTTCGTCTTCTTCATCCATGCAAACTCTCAGTCCCTTCAAGACAAGGAATTATCAATCCTGCTTAAATTAAAGTCATATTGGCTATATCCACCAGCTCTCAGTCATTGGACTTCATCATCAAACTCTGGTTCCCACTGTAACTGGCCTGAGATCAACTGCACCGGAAACTCCGTCACCGGATTGAATCTCAACACCGTAAACCTCACCCTTAAAGTTCCACCCTTCATTTGTGACCTCAACAACCTCACACACCTTGACCTTGGTAATAACTACCTCCCGGGAGATTTTCCCTTATCTCTCTACAGCTGCTCCGAGCTAGAGTATCTTGACCTCTCGCAAAACTACTTTGTGGGTACAATTCCTGATGACATCGACAGACTGCCTAAGCTTAAGATCCTCAACCTTGCTGGCAACAACTTCTCCGGTGACATTCCGGCGTCTATTGGGAACTTACAGGAGCTGGCTACTTTGGATATATACATGAACCAGCTCAACGGCTCTGTCCCAGCTGAAATAGGTAACCTCTCCAATCTTGAGCAACTCTGGCTCTCTTGGCTTCCCAAAATGGCGCCCTGGAAGCTGCCTTTTAACTTCACTGAGTTGAAAAAGATGAAGATATTGAAGATACGCGAAGCAAATTTGATTGGGGAAATACCAGAATCTATTGGAGAATTGGAAGCACTTGAAGTATTGGATTTGGCGCAGAACAAGTTGAGCGGGAAAGTACCAAGCGGTGTGTTTCTGTTGAAGAATTTGAGCATTATATATCTGTTCAAGAACAGGCTTTCAGGGGAGATTCCTCAAGTGGTTGAAGCACTCAACTTGAGCATCGTTGACATCTCAGAAAACAATCTCACAGGGCCGATACCAGAAGGTTTCGGAAACCTTGCCAAGTTAACAGATATGAGTTTGTTCTACAATGGTATTTCAGGTGAGATTCCAGAGAGCATTGGCCGCCTGCCAAAGCTTGTGATTTTCAAAATGTTCAACATGAACTTGTCAGGAACAATTCCTCCAGAGCTTGGAAAGCATTCTCTGCTTGAAGATTTCCAGGTTTCGGTGAATAGCCTTACCGGAAAGCTGCCAGACGGCTTGTGTAAAAATGGTAAGCTTGTGGCAGTTATTGCTTATGAGAATAGTCTCACTGGGGAGCTACCAAGCTCTCTTGGAAATTGTGATAGTCTGCTGATAGTCAATGTCTATGATAACATGCTATCTGGGGACATTCCTAGTGGTATGTGGAATGCATTGAATTTGAGTTATGTGATGATGAGCAACAATTCGTTCACGGGTGAGCTTCCTGAGAAGATGTCATATAATCTTCAAAGGCTGGAAATCGGAGGTAACAAGTTTTCAGGTAAGATTCCAAGAGGGGTGTCTTCCTGTAAGAATCTGCAAGTTTTTGATGCTGGTAACAACCTCTTCAACGGTAGTATTCCTCAGGAATTAACTACTCTTCGGAGCTTGACGACTCTGACCCTGCATCAGAATCAGCTTAGCGGGCTACTTCCATCAGATATATCATGGAAGTCACTAAACACTCTTGATCTGCGTAGAAATCAACTCTCCGGACCAATTCCAGAGATACTTGGTGGTTTGCCCGCCCTTACTGAATTGGACCTTTCTGAAAACCTGTTTTCAGGCCAAATTCCAAATCAACTTGGCCATCTGAAACTCAATAAGTTCAACCTCTCTTCAAATCTCCTCTCTGGCGAGATCCCAATTTCATTTGAAAACTCTGCGTATGAGAGAAGCTTCTTGAACAACACCGGTCTCTGTGCAAGCACCAGTGGTGTGAATCTCAATATATGCTCTCAATCGCCAGAGTCCAGCAAGATTTCAATTCGGTCTCTTGCTTTGATCTTAAGTCTAAGCGTGGTGTTGTTCGTGTTGGTTTTGTCCTTATTAATCTTCTTTGTTAGAGGTTACTTGAGGAGGAAGGATGGATCAGAAGCAGACTGGAAGCTCGTTGCATTTCAGAGGTTGAATTTCACCTGCTCAAATATCTTGTCAGGGTTGACAGAAGGCAATGTGATTGGAAGTGGTGGATCAGGGAAGGTTTATCGTGTACCTGTGAATCGTACAGGTGATGTTGTAGCTGCGAAGAGGATTTGGACCAACAAGAAGATCATGGAGGAGAGACTTGAAAAAGAGTTCCTTGCAGAAGTCAAGATACTGAGCTCAATTCGACATGCCAACATAGTGAAGCTGATGTGCTGCATATCCAGTGAGACTTCAAAACTTCTTGTATACGAGTACTTAGACAATCGAAGCCTAGATCGATGGTTGCACAAGAAAAACAGGCCATACCAATCCAACCTCTCAAGTTCAGTCCATCATGTTGTTCTGGACTGGCCTAAGAGGTTGCAGATTGCAGTGGGAGTTGCTAAGGCCCTCTGCTATATGCATCATGACTGTGTTCCACCCGTTGTGCATCGAGACGTGAAATCAAGTAACGTTTTATTGGACTCTGATTTCAACGCAAAAATAGCAGATTTTGGTCTGGCCAAGATGTTGGTCAAGCAAGGAGACCTTGCCACAATGTCAGCTGTTGCTGGTTCCTTTGGCTACATGGCTCCAGAATATGCACATACCAGAAGAGTGAATGAAAAGATCGATGTATATAGTTTCGGGGTCATCCTGCTGGAGTTGGCAACTGGTAGGGAACCCAATGAGGGTGATGAAAACACTTCTCTCGCCGAATGGGCGTGGCGTCGTGCTCAAGAAGGCAAACCTATCGCCAGTGCTCTGGACCAGGACGTCAAAGAACCCTGTTACTTGGATGAAATGTGCGCTGTTTTCAGACTCGGCATCACGTGCACCGAGACACTTCCTACTAATAGGCCTCCTATGAAGGAGGTTCTGCAGATGTTGGTTCGAACTTGTCCTCCACCAGTTCGCAGGGAAAAGACTGAATATGCCGCTGCTCCTCTCCTCAAGAACTCGAGGCGAGAGAGAGTTTTGGAAGATCAAGATGGTTTGGCAACGAATGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0010015 GO:0010101 GO:0010102 GO:0010468 GO:0019222 GO:0022622 GO:0032501 GO:0032502 GO:0048364 GO:0048527 GO:0048528 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0065007 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1013

Amino Acids

112.14

Weight (kDa)

6.18

Isoelectric Point (pI)

41.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	96 - 155	2.2e-09	Leucine rich repeat
LRR_14	PF23598	116 - 224	1.4e-10	Leucine-rich repeat region
LRR_14	PF23598	214 - 287	2.8e-07	Leucine-rich repeat region
LRR_8	PF13855	526 - 584	3.5e-06	Leucine rich repeat
Pkinase	PF00069	690 - 971	5.5e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	690 - 973	2.2e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001321)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25930
fragaria_vesca	FvH4_1g06180 FvH4_1g06180 FvH4_1g06220 FvH4_1g06260 FvH4_1g07450 FvH4_1g07450 FvH4_1g07450 FvH4_2g35260
malus_domestica	MD02G1066200.v1.1 MD02G1066300.v1.1 MD02G1066400.v1.1 MD08G1110500.v1.1 MD15G1196900.v1.1
prunus_persica	Prupe.1G444700_v2.0.a1 Prupe.7G218900_v2.0.a1 Prupe.7G219100_v2.0.a1 Prupe.7G219300_v2.0.a1 Prupe.7G219300_v2.0.a1 Prupe.7G219400_v2.0.a1 Prupe.7G219500_v2.0.a1
pyrus_communis	pycom02g05250 pycom08g08890 pycom08g09200 pycom15g08430 pycom15g17590
rosa_chinensis	RchiOBHm_Chr2g0091961 RchiOBHm_Chr6g0306681
rosa_laevigata	RLG00000010774 RLG00000016262 RLG00000016266
rosa_multiflora	Rmu_co7980064.1_g000001 Rmu_sc0005201.1_g000003 Rmu_sc0009216.1_g000004 Rmu_sc0014758.1_g000001 Rmu_sc0017125.1_g000005 Rmu_sc0017648.1_g000001 Rmu_sc0017649.1_g000001 Rmu_sc0017785.1_g000010 Rmu_sc0017785.1_g000011
rosa_roxburghii	Rroxscaffold_2G00149580 Rroxscaffold_7G00161550
rosa_rugosa	Rorug02G0022000 Rorug06G0351600
rosa_samantha	Rh2AG067500 Rh2BG066800 Rh2CG068100 Rh2DG066600 Rh6AG463900 Rh6BG437100 Rh6CG478000 Rh6DG464800
rosa_wichuraiana	Rw2G005350 Rw6G040410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2048
AasI	GACNNNNNNGTC	1 cut(s)	2804
AatII	GACGTC	1 cut(s)	2808
Acc36I	ACCTGC	1 cut(s)	2048
AccB1I	GGYRCC	1 cut(s)	613
AccB7I	CCANNNNNTGG	1 cut(s)	1067
AccBSI	CCGCTC	1 cut(s)	762
AccI	GTMKAC	1 cut(s)	2295
AccII	CGCG	1 cut(s)	675
AccIII	TCCGGA	1 cut(s)	1613
AciI	CCGC	6 cut(s)	762, 777, 1000, 1545, 1647, 2967
AclI	AACGTT	1 cut(s)	2502
AclWI	GGATC	4 cut(s)	433, 1756, 1999, 2101
AcoI	YGGCCR	3 cut(s)	997, 1708, 2550
AcsI	RAATTY	5 cut(s)	682, 796, 1297, 1692, 2032
AcvI	CACGTG	1 cut(s)	2862
AcyI	GRCGYC	4 cut(s)	482, 614, 2758, 2805
AfaI	GTAC	5 cut(s)	400, 771, 2115, 2128, 2303
AfiI	CCNNNNNNNGG	3 cut(s)	1067, 1102, 2720
AflII	CTTAAG	2 cut(s)	434, 1909
AgeI	ACCGGT	1 cut(s)	1814
AjiI	CACGTC	1 cut(s)	2488
AjnI	CCWGG	3 cut(s)	617, 1095, 2799
AloI	GAACNNNNNNTCC	4 cut(s)	1042, 1074, 2917, 2949
Alw21I	GWGCWC	4 cut(s)	2228, 2767, 2794, 2867
Alw26I	GTCTC	8 cut(s)	1190, 1823, 1899, 2179, 2270, 2478, 2568, 2864
Alw44I	GTGCAC	1 cut(s)	2863
AlwI	GGATC	4 cut(s)	433, 1756, 1999, 2101
AlwNI	CAGNNNCTG	1 cut(s)	505
Ama87I	CYCGRG	2 cut(s)	322, 2989
Aor13HI	TCCGGA	1 cut(s)	1613
ApaLI	GTGCAC	1 cut(s)	2863
ApeKI	GCWGC	6 cut(s)	351, 625, 1126, 2144, 2262, 2969
ApoI	RAATTY	5 cut(s)	682, 796, 1297, 1692, 2032
ArsI	GACNNNNNNTTYG	4 cut(s)	59, 91, 2039, 2071
AseI	ATTAAT	1 cut(s)	1952
AsiGI	ACCGGT	1 cut(s)	1814
Asp700I	GAANNNNTTC	1 cut(s)	1075
AspLEI	GCGC	3 cut(s)	616, 748, 2840
AspS9I	GGNCC	6 cut(s)	905, 1616, 1663, 2160, 2437, 2797
AsuC2I	CCSGG	2 cut(s)	323, 324
AsuHPI	GGTGA	9 cut(s)	211, 241, 482, 989, 1117, 1349, 2029, 2144, 2735
AsuII	TTCGAA	1 cut(s)	2923
AvaI	CYCGRG	2 cut(s)	322, 2989
AvaII	GGWCC	4 cut(s)	1616, 1663, 2160, 2797
AxyI	CCTNAGG	1 cut(s)	1485
BaeGI	GKGCMC	1 cut(s)	2867
BalI	TGGCCA	2 cut(s)	1710, 2552
BanI	GGYRCC	1 cut(s)	613
BanII	GRGCYC	1 cut(s)	2228
BarI	GAAGNNNNNNTAC	2 cut(s)	2202, 2234
BbrPI	CACGTG	1 cut(s)	2862
BbsI	GAAGAC	2 cut(s)	43, 1416
Bbv12I	GWGCWC	4 cut(s)	2228, 2767, 2794, 2867
BbvI	GCAGC	6 cut(s)	338, 612, 1113, 2131, 2249, 2956
BccI	CCATC	6 cut(s)	1564, 1719, 1982, 2322, 2388, 3014
BceAI	ACGGC	2 cut(s)	556, 1150
BcgI	CGANNNNNNTGC	2 cut(s)	2764, 2798
BciT130I	CCWGG	3 cut(s)	619, 1097, 2801
BcnI	CCSGG	2 cut(s)	323, 324
BcoDI	GTCTC	8 cut(s)	1190, 1823, 1899, 2179, 2270, 2478, 2568, 2864
BfaI	CTAG	3 cut(s)	362, 1275, 2321
BfmI	CTRYAG	2 cut(s)	346, 2909
BfoI	RGCGCY	1 cut(s)	617
BfrI	CTTAAG	2 cut(s)	434, 1909
BfuAI	ACCTGC	1 cut(s)	2048
BglI	GCCNNNNNGGC	1 cut(s)	2750
BisI	GCNGC	8 cut(s)	352, 626, 1000, 1127, 2145, 2263, 2967, 2970
BlpI	GCTNAGC	1 cut(s)	1541
BlsI	GCNGC	8 cut(s)	353, 627, 1001, 1128, 2146, 2264, 2968, 2971
BmcAI	AGTACT	1 cut(s)	2303
Bme1390I	CCNGG	5 cut(s)	323, 324, 619, 1097, 2801
Bme18I	GGWCC	4 cut(s)	1616, 1663, 2160, 2797
BmeT110I	CYCGRG	2 cut(s)	322, 2989
BmgBI	CACGTC	1 cut(s)	2488
BmgT120I	GGNCC	6 cut(s)	905, 1616, 1663, 2160, 2437, 2797
BmiI	GGNNCC	5 cut(s)	175, 615, 2605, 2622, 2712
BmrFI	CCNGG	5 cut(s)	323, 324, 619, 1097, 2801
BmrI	ACTGGG	1 cut(s)	1200
BmsI	GCATC	6 cut(s)	882, 1441, 1537, 2461, 2488, 2865
BmuI	ACTGGG	1 cut(s)	1200
BpiI	GAAGAC	2 cut(s)	43, 1416
BpmI	CTGGAG	3 cut(s)	1044, 2607, 2711
Bpu10I	CCTNAGC	2 cut(s)	429, 2433
Bpu1102I	GCTNAGC	1 cut(s)	1541
Bpu14I	TTCGAA	1 cut(s)	2923
BpuEI	CTTGAG	7 cut(s)	599, 831, 889, 1996, 2353, 2751, 2966
BpuMI	CCSGG	2 cut(s)	323, 324
Bsa29I	ATCGAT	2 cut(s)	2326, 2661
BsaAI	YACGTR	1 cut(s)	2862
BsaHI	GRCGYC	4 cut(s)	482, 614, 2758, 2805
BsaI	GGTCTC	3 cut(s)	1823, 1899, 2568
BsaJI	CCNNGG	3 cut(s)	304, 322, 617
BsaWI	WCCGGW	6 cut(s)	206, 221, 467, 1118, 1613, 1814
BsaXI	ACNNNNNCTCC	8 cut(s)	338, 368, 449, 479, 1042, 1072, 2917, 2947
Bsc4I	CCNNNNNNNGG	3 cut(s)	1067, 1102, 2720
Bse118I	RCCGGY	1 cut(s)	1814
Bse1I	ACTGG	9 cut(s)	191, 1195, 1833, 2009, 2271, 2402, 2707, 2787, 2939
Bse21I	CCTNAGG	1 cut(s)	1485
Bse3DI	GCAATG	1 cut(s)	2080
BseAI	TCCGGA	1 cut(s)	1613
BseBI	CCWGG	3 cut(s)	619, 1097, 2801
BseCI	ATCGAT	2 cut(s)	2326, 2661
BseDI	CCNNGG	3 cut(s)	304, 322, 617
BseGI	GGATG	4 cut(s)	57, 1993, 2682, 2833
BseLI	CCNNNNNNNGG	3 cut(s)	1067, 1102, 2720
BseMI	GCAATG	1 cut(s)	2080
BseMII	CTCAG	8 cut(s)	86, 157, 183, 633, 900, 1338, 1499, 2213
BseNI	ACTGG	9 cut(s)	191, 1195, 1833, 2009, 2271, 2402, 2707, 2787, 2939
BseRI	GAGGAG	5 cut(s)	1739, 1993, 2195, 2964, 2969
BseSI	GKGCMC	1 cut(s)	2867
BseXI	GCAGC	6 cut(s)	338, 612, 1113, 2131, 2249, 2956
BseYI	CCCAGC	1 cut(s)	550
BsgI	GTGCAG	1 cut(s)	187
Bsh1236I	CGCG	1 cut(s)	675
BshNI	GGYRCC	1 cut(s)	613
BshTI	ACCGGT	1 cut(s)	1814
BshVI	ATCGAT	2 cut(s)	2326, 2661
BsiHKAI	GWGCWC	4 cut(s)	2228, 2767, 2794, 2867
BsiHKCI	CYCGRG	2 cut(s)	322, 2989
BsiSI	CCGG	8 cut(s)	207, 222, 323, 468, 479, 1119, 1614, 1815
BslFI	GGGAC	3 cut(s)	61, 533, 1280
BslI	CCNNNNNNNGG	3 cut(s)	1067, 1102, 2720
BsmAI	GTCTC	8 cut(s)	1190, 1823, 1899, 2179, 2270, 2478, 2568, 2864
BsmBI	CGTCTC	1 cut(s)	2478
BsmFI	GGGAC	3 cut(s)	61, 533, 1280
BsmI	GAATGC	2 cut(s)	1075, 1294
Bso31I	GGTCTC	3 cut(s)	1823, 1899, 2568
BsoBI	CYCGRG	2 cut(s)	322, 2989
Bsp119I	TTCGAA	1 cut(s)	2923
Bsp1286I	GDGCHC	4 cut(s)	2228, 2767, 2794, 2867
Bsp13I	TCCGGA	1 cut(s)	1613
Bsp1720I	GCTNAGC	1 cut(s)	1541
BspACI	CCGC	6 cut(s)	762, 777, 1000, 1545, 1647, 2967
BspCNI	CTCAG	8 cut(s)	85, 156, 184, 634, 899, 1339, 1498, 2214
BspDI	ATCGAT	2 cut(s)	2326, 2661
BspEI	TCCGGA	1 cut(s)	1613
BspFNI	CGCG	1 cut(s)	675
BspHI	TCATGA	1 cut(s)	2455
BspLI	GGNNCC	5 cut(s)	175, 615, 2605, 2622, 2712
BspMAI	CTGCAG	1 cut(s)	2913
BspMI	ACCTGC	1 cut(s)	2048
BspPI	GGATC	4 cut(s)	433, 1756, 1999, 2101
BspT104I	TTCGAA	1 cut(s)	2923
BspT107I	GGYRCC	1 cut(s)	613
BspTI	CTTAAG	2 cut(s)	434, 1909
BspTNI	GGTCTC	3 cut(s)	1823, 1899, 2568
BsrBI	CCGCTC	1 cut(s)	762
BsrDI	GCAATG	1 cut(s)	2080
BsrFI	RCCGGY	1 cut(s)	1814
BsrI	ACTGG	9 cut(s)	191, 1195, 1833, 2009, 2271, 2402, 2707, 2787, 2939
BssAI	RCCGGY	1 cut(s)	1814
BssECI	CCNNGG	3 cut(s)	304, 322, 617
BssNAI	GTATAC	1 cut(s)	2296
BssNI	GRCGYC	4 cut(s)	482, 614, 2758, 2805
BssT1I	CCWWGG	1 cut(s)	304
Bst1107I	GTATAC	1 cut(s)	2296
Bst2UI	CCWGG	3 cut(s)	619, 1097, 2801
Bst4CI	ACNGT	4 cut(s)	182, 241, 1475, 2462
Bst6I	CTCTTC	5 cut(s)	1472, 1506, 1744, 2144, 2638
BstACI	GRCGYC	4 cut(s)	482, 614, 2758, 2805
BstAFI	CTTAAG	2 cut(s)	434, 1909
BstAPI	GCANNNNNTGC	3 cut(s)	1081, 1169, 2862
BstBAI	YACGTR	1 cut(s)	2862
BstBI	TTCGAA	1 cut(s)	2923
BstC8I	GCNNGC	6 cut(s)	454, 507, 1004, 1547, 1647, 1828
BstEII	GGTNACC	1 cut(s)	563
BstF5I	GGATG	4 cut(s)	57, 1993, 2682, 2833
BstFNI	CGCG	1 cut(s)	675
BstH2I	RGCGCY	1 cut(s)	617
BstHHI	GCGC	3 cut(s)	616, 748, 2840
BstMAI	GTCTC	8 cut(s)	1190, 1823, 1899, 2179, 2270, 2478, 2568, 2864
BstNI	CCWGG	3 cut(s)	619, 1097, 2801
BstNSI	RCATGY	2 cut(s)	1258, 2240
BstPI	GGTNACC	1 cut(s)	563
BstSCI	CCNGG	5 cut(s)	321, 322, 617, 1095, 2799
BstSFI	CTRYAG	2 cut(s)	346, 2909
BstSLI	GKGCMC	1 cut(s)	2867
BstUI	CGCG	1 cut(s)	675
BstV1I	GCAGC	6 cut(s)	338, 612, 1113, 2131, 2249, 2956
BstV2I	GAAGAC	2 cut(s)	43, 1416
BstX2I	RGATCY	2 cut(s)	438, 1761
BstXI	CCANNNNNNTGG	2 cut(s)	2560, 2794
BstYI	RGATCY	2 cut(s)	438, 1761
BstZ17I	GTATAC	1 cut(s)	2296
Bsu15I	ATCGAT	2 cut(s)	2326, 2661
Bsu36I	CCTNAGG	1 cut(s)	1485
BsuTUI	ATCGAT	2 cut(s)	2326, 2661
BtrI	CACGTC	1 cut(s)	2488
BtsCI	GGATG	4 cut(s)	57, 1993, 2682, 2833
BtsI	GCAGTG	1 cut(s)	2427
BtsIMutI	CAGTG	7 cut(s)	178, 639, 1188, 1840, 2278, 2427, 2794
BveI	ACCTGC	1 cut(s)	2048
Cac8I	GCNNGC	6 cut(s)	454, 507, 1004, 1547, 1647, 1828
CaiI	CAGNNNCTG	1 cut(s)	505
CciI	TCATGA	1 cut(s)	2455
CfoI	GCGC	3 cut(s)	616, 748, 2840
Cfr10I	RCCGGY	1 cut(s)	1814
Cfr13I	GGNCC	6 cut(s)	905, 1616, 1663, 2160, 2437, 2797
Cfr9I	CCCGGG	1 cut(s)	322
ClaI	ATCGAT	2 cut(s)	2326, 2661
CseI	GACGC	2 cut(s)	471, 2747
Csp6I	GTAC	5 cut(s)	399, 770, 2114, 2127, 2302
CspAI	ACCGGT	1 cut(s)	1814
CviQI	GTAC	5 cut(s)	399, 770, 2114, 2127, 2302
DinI	GGCGCC	1 cut(s)	615
DrdI	GACNNNNNNGTC	1 cut(s)	2804
DseDI	GACNNNNNNGTC	1 cut(s)	2804
EaeI	YGGCCR	3 cut(s)	997, 1708, 2550
Eam1104I	CTCTTC	5 cut(s)	1472, 1506, 1744, 2144, 2638
EarI	CTCTTC	5 cut(s)	1472, 1506, 1744, 2144, 2638
Ecl136II	GAGCTC	1 cut(s)	2226
Eco130I	CCWWGG	1 cut(s)	304
Eco147I	AGGCCT	1 cut(s)	2890
Eco24I	GRGCYC	1 cut(s)	2228
Eco31I	GGTCTC	3 cut(s)	1823, 1899, 2568
Eco47I	GGWCC	4 cut(s)	1616, 1663, 2160, 2797
Eco53kI	GAGCTC	1 cut(s)	2226
Eco72I	CACGTG	1 cut(s)	2862
Eco81I	CCTNAGG	1 cut(s)	1485
Eco88I	CYCGRG	2 cut(s)	322, 2989
Eco91I	GGTNACC	1 cut(s)	563
EcoICRI	GAGCTC	1 cut(s)	2226
EcoO109I	RGGNCCY	1 cut(s)	2437
EcoO65I	GGTNACC	1 cut(s)	563
EcoRII	CCWGG	3 cut(s)	617, 1095, 2799
EcoT14I	CCWWGG	1 cut(s)	304
EcoT22I	ATGCAT	2 cut(s)	1294, 2454
EcoT38I	GRGCYC	1 cut(s)	2228
EgeI	GGCGCC	1 cut(s)	615
EheI	GGCGCC	1 cut(s)	615
ErhI	CCWWGG	1 cut(s)	304
Esp3I	CGTCTC	1 cut(s)	2478
FalI	AAGNNNNNCTT	4 cut(s)	2187, 2219, 2562, 2594
FaqI	GGGAC	3 cut(s)	61, 533, 1280
FauI	CCCGC	3 cut(s)	755, 1538, 1654
FblI	GTMKAC	1 cut(s)	2295
Fnu4HI	GCNGC	8 cut(s)	352, 626, 1000, 1127, 2145, 2263, 2967, 2970
FokI	GGATG	4 cut(s)	44, 2000, 2669, 2840
FriOI	GRGCYC	1 cut(s)	2228
Fsp4HI	GCNGC	8 cut(s)	352, 626, 1000, 1127, 2145, 2263, 2967, 2970
FspBI	CTAG	3 cut(s)	362, 1275, 2321
GlaI	GCGC	3 cut(s)	615, 747, 2839
GluI	GCNGC	8 cut(s)	352, 626, 1000, 1127, 2145, 2263, 2967, 2970
GsaI	CCCAGC	1 cut(s)	554
GsuI	CTGGAG	3 cut(s)	1044, 2607, 2711
HaeII	RGCGCY	1 cut(s)	617
HapII	CCGG	8 cut(s)	207, 222, 323, 468, 479, 1119, 1614, 1815
HgaI	GACGC	2 cut(s)	471, 2747
HhaI	GCGC	3 cut(s)	616, 748, 2840
Hin1I	GRCGYC	4 cut(s)	482, 614, 2758, 2805
Hin6I	GCGC	3 cut(s)	614, 746, 2838
HinP1I	GCGC	3 cut(s)	614, 746, 2838
HincII	GTYRAC	3 cut(s)	880, 942, 2064
HindII	GTYRAC	3 cut(s)	880, 942, 2064
HindIII	AAGCTT	4 cut(s)	431, 1010, 1154, 1798
HpaI	GTTAAC	1 cut(s)	942
HpaII	CCGG	8 cut(s)	207, 222, 323, 468, 479, 1119, 1614, 1815
HphI	GGTGA	9 cut(s)	211, 241, 482, 989, 1117, 1349, 2029, 2144, 2735
Hpy166II	GTNNAC	8 cut(s)	244, 880, 942, 1332, 2064, 2114, 2296, 2865
Hpy8I	GTNNAC	8 cut(s)	244, 880, 942, 1332, 2064, 2114, 2296, 2865
Hpy99I	CGWCG	1 cut(s)	2763
HpyAV	CCTTC	8 cut(s)	89, 277, 911, 1978, 2063, 2095, 2765, 2893
HpyCH4III	ACNGT	4 cut(s)	182, 241, 1475, 2462
HpyCH4IV	ACGT	4 cut(s)	2487, 2502, 2805, 2861
HpySE526I	ACGT	4 cut(s)	2487, 2502, 2805, 2861
Hsp92I	GRCGYC	4 cut(s)	482, 614, 2758, 2805
HspAI	GCGC	3 cut(s)	614, 746, 2838
KasI	GGCGCC	1 cut(s)	613
Kpn2I	TCCGGA	1 cut(s)	1613
KspAI	GTTAAC	1 cut(s)	942
LmnI	GCTCC	6 cut(s)	359, 502, 1195, 1506, 2626, 2977
Lsp1109I	GCAGC	6 cut(s)	338, 612, 1113, 2131, 2249, 2956
LweI	GCATC	6 cut(s)	882, 1441, 1537, 2461, 2488, 2865
MaeI	CTAG	3 cut(s)	362, 1275, 2321
MaeII	ACGT	4 cut(s)	2487, 2502, 2805, 2861
MbiI	CCGCTC	1 cut(s)	762
MflI	RGATCY	2 cut(s)	438, 1761
MhlI	GDGCHC	4 cut(s)	2228, 2767, 2794, 2867
MlsI	TGGCCA	2 cut(s)	1710, 2552
MluNI	TGGCCA	2 cut(s)	1710, 2552
Mly113I	GGCGCC	1 cut(s)	614
MlyI	GAGTC	4 cut(s)	1510, 1880, 2507, 2844
MmeI	TCCRAC	1 cut(s)	2385
Mox20I	TGGCCA	2 cut(s)	1710, 2552
Mph1103I	ATGCAT	2 cut(s)	1294, 2454
MroI	TCCGGA	1 cut(s)	1613
MroXI	GAANNNNTTC	1 cut(s)	1075
MscI	TGGCCA	2 cut(s)	1710, 2552
MseI	TTAA	9 cut(s)	108, 113, 255, 435, 633, 941, 1493, 1910, 1952
MslI	CAYNNNNRTG	1 cut(s)	2460
Msp20I	TGGCCA	2 cut(s)	1710, 2552
MspA1I	CMGCKG	4 cut(s)	351, 554, 2594, 2969
MspCI	CTTAAG	2 cut(s)	434, 1909
MspI	CCGG	8 cut(s)	207, 222, 323, 468, 479, 1119, 1614, 1815
MspR9I	CCNGG	5 cut(s)	323, 324, 619, 1097, 2801
Mva1269I	GAATGC	2 cut(s)	1075, 1294
MvaI	CCWGG	3 cut(s)	619, 1097, 2801
MvnI	CGCG	1 cut(s)	675
NarI	GGCGCC	1 cut(s)	614
NciI	CCSGG	2 cut(s)	323, 324
NlaIV	GGNNCC	5 cut(s)	175, 615, 2605, 2622, 2712
NmeAIII	GCCGAG	1 cut(s)	2832
NmuCI	GTSAC	4 cut(s)	217, 275, 470, 1575
NsiI	ATGCAT	2 cut(s)	1294, 2454
NspI	RCATGY	2 cut(s)	1258, 2240
NspV	TTCGAA	1 cut(s)	2923
PaeR7I	CTCGAG	1 cut(s)	2989
PagI	TCATGA	1 cut(s)	2455
PaqCI	CACCTGC	1 cut(s)	2048
PceI	AGGCCT	1 cut(s)	2890
PctI	GAATGC	2 cut(s)	1075, 1294
PdmI	GAANNNNTTC	1 cut(s)	1075
PfeI	GAWTC	9 cut(s)	229, 704, 841, 982, 1408, 1435, 1534, 1843, 2122
PflMI	CCANNNNNTGG	1 cut(s)	1067
PinAI	ACCGGT	1 cut(s)	1814
PkrI	GCNGC	8 cut(s)	353, 627, 1001, 1128, 2146, 2264, 2968, 2971
PleI	GAGTC	4 cut(s)	1510, 1879, 2507, 2844
PluTI	GGCGCC	1 cut(s)	617
PmaCI	CACGTG	1 cut(s)	2862
PmlI	CACGTG	1 cut(s)	2862
PpsI	GAGTC	4 cut(s)	1510, 1879, 2507, 2844
Ppu21I	YACGTR	1 cut(s)	2862
PshBI	ATTAAT	1 cut(s)	1952
Psp124BI	GAGCTC	1 cut(s)	2228
Psp1406I	AACGTT	1 cut(s)	2502
Psp6I	CCWGG	3 cut(s)	617, 1095, 2799
PspCI	CACGTG	1 cut(s)	2862
PspEI	GGTNACC	1 cut(s)	563
PspFI	CCCAGC	1 cut(s)	550
PspGI	CCWGG	3 cut(s)	617, 1095, 2799
PspN4I	GGNNCC	5 cut(s)	175, 615, 2605, 2622, 2712
PspPI	GGNCC	6 cut(s)	905, 1616, 1663, 2160, 2437, 2797
PspXI	VCTCGAGB	1 cut(s)	2989
PstI	CTGCAG	1 cut(s)	2913
PstNI	CAGNNNCTG	1 cut(s)	505
PsuI	RGATCY	2 cut(s)	438, 1761
PvuII	CAGCTG	3 cut(s)	351, 554, 2594
RsaI	GTAC	5 cut(s)	400, 771, 2115, 2128, 2303
RsaNI	GTAC	5 cut(s)	399, 770, 2114, 2127, 2302
RseI	CAYNNNNRTG	1 cut(s)	2460
SacI	GAGCTC	1 cut(s)	2228
SaqAI	TTAA	9 cut(s)	108, 113, 255, 435, 633, 941, 1493, 1910, 1952
SatI	GCNGC	8 cut(s)	352, 626, 1000, 1127, 2145, 2263, 2967, 2970
Sau96I	GGNCC	6 cut(s)	905, 1616, 1663, 2160, 2437, 2797
ScaI	AGTACT	1 cut(s)	2303
SchI	GAGTC	4 cut(s)	1510, 1880, 2507, 2844
ScrFI	CCNGG	5 cut(s)	323, 324, 619, 1097, 2801
SduI	GDGCHC	4 cut(s)	2228, 2767, 2794, 2867
SfaNI	GCATC	6 cut(s)	882, 1441, 1537, 2461, 2488, 2865
SfcI	CTRYAG	2 cut(s)	346, 2909
SfoI	GGCGCC	1 cut(s)	615
Sfr274I	CTCGAG	1 cut(s)	2989
SfuI	TTCGAA	1 cut(s)	2923
SinI	GGWCC	4 cut(s)	1616, 1663, 2160, 2797
SlaI	CTCGAG	1 cut(s)	2989
SmaI	CCCGGG	1 cut(s)	324
SmiMI	CAYNNNNRTG	1 cut(s)	2460
SseBI	AGGCCT	1 cut(s)	2890
SsiI	CCGC	6 cut(s)	762, 777, 1000, 1545, 1647, 2967
SspDI	GGCGCC	1 cut(s)	613
SspMI	CTAG	3 cut(s)	362, 1275, 2321
SstI	GAGCTC	1 cut(s)	2228
StuI	AGGCCT	1 cut(s)	2890
StyD4I	CCNGG	5 cut(s)	321, 322, 617, 1095, 2799
StyI	CCWWGG	1 cut(s)	304
TaaI	ACNGT	4 cut(s)	182, 241, 1475, 2462
TaiI	ACGT	4 cut(s)	2490, 2505, 2808, 2864
TaqI	TCGA	8 cut(s)	417, 2233, 2314, 2326, 2482, 2661, 2923, 2990
TaqII	GACCGA	1 cut(s)	1881
TatI	WGTACW	1 cut(s)	2301
TauI	GCSGC	2 cut(s)	1002, 2969
TfiI	GAWTC	9 cut(s)	229, 704, 841, 982, 1408, 1435, 1534, 1843, 2122
Tru1I	TTAA	9 cut(s)	108, 113, 255, 435, 633, 941, 1493, 1910, 1952
Tru9I	TTAA	9 cut(s)	108, 113, 255, 435, 633, 941, 1493, 1910, 1952
TscAI	CASTG	7 cut(s)	185, 646, 1195, 1840, 2278, 2427, 2794
TseFI	GTSAC	4 cut(s)	217, 275, 470, 1575
TseI	GCWGC	6 cut(s)	351, 625, 1126, 2144, 2262, 2969
Tsp45I	GTSAC	4 cut(s)	217, 275, 470, 1575
TspGWI	ACGGA	1 cut(s)	205
TspMI	CCCGGG	1 cut(s)	322
TspRI	CASTG	7 cut(s)	185, 646, 1195, 1840, 2278, 2427, 2794
Van91I	CCANNNNNTGG	1 cut(s)	1067
Vha464I	CTTAAG	2 cut(s)	434, 1909
VneI	GTGCAC	1 cut(s)	2863
VpaK11BI	GGWCC	4 cut(s)	1616, 1663, 2160, 2797
VspI	ATTAAT	1 cut(s)	1952
XapI	RAATTY	5 cut(s)	682, 796, 1297, 1692, 2032
XceI	RCATGY	2 cut(s)	1258, 2240
XhoI	CTCGAG	1 cut(s)	2989
XmaI	CCCGGG	1 cut(s)	322
XmiI	GTMKAC	1 cut(s)	2295
XmnI	GAANNNNTTC	1 cut(s)	1075
XspI	CTAG	3 cut(s)	362, 1275, 2321
ZraI	GACGTC	1 cut(s)	2806
ZrmI	AGTACT	1 cut(s)	2303
Zsp2I	ATGCAT	2 cut(s)	1294, 2454

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.