Rroxscaffold_7G00161550
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001321
Physical Location & Seq
Reverse (-)
4286399 .. 4289965
3567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161550.1

Sequence Viewer

Length: 1179 bp
ATGATGAGCAACAATTCGCTTTCGGGGCAGCTTCCGAGGACAAGGCTAGCTTCGAATCTGTCTAGACTGGAAATCAGCAACAATAAATTTTCTGGTGAAATTCCAGTTCACGTTTCATCTTGGCAAACTTTGGTTGTTTTTAAGGCAAGTGGGAATCTGTTTTCAGGTAAAATCCCAGTAGAATTGACAAGTCTTTCACAGCTGAACACTTTGTCACTCGATGGGAATCGATTTTCGGGTGAATTACCAACGCAGATCATTGCGTGGGGATCATTGACTACTCTAGATCTCTCAAGAAATGAGCTTTCAGGCTACATTCCAACAGCTATTGGTTCTTTGCCTGACCTCCTCTACTTGGATTTGTCAGGAAACAAATTCTCAGGTCAAATCCCATCTGAGTTAGGCCACTTGAGGCTCAACTCCCTCAACTTGTCTTCAAACGAGCTTTCTGGGAAGATTCCAGATGTGTTTGATAACCTTGCTTATCAAAACAGTTTCTTGAACAATTCAAATCTATGTGCTAATAGTCCAATTCTAAACCTTCCCAGTTGCTACACCAAAATCCATAGTTCCCACAAGTTGTCCTCCAGAGTCCTTGCCATGATCTTAGTCCTCTCCATTGTTGTGTTTCTAGTTACAGTTCTGTTGACATTTTTCGTGGTCAGAGACTACCGGAGGAAGAAGCGTGGCCATGACTTGGCAACATGGAAGCTTACCTCATTCCAGAGGTTGGATTTCACTGAGTTCAATGTCTTGACAAACTTGACAGATACTAATCTCATTGGAAGTGGAGGCTCAGGGAAGGTTTATCGAGTTAGTACTAACTGCCCACGTGAATTTGTCGCGGTTAAAAGGATTTGGAACAGAAAGGAACTAGATCAGAAGCTCGAGAAAGAATTCAATGCTGAGGTTGAGATACTGGGCACCATTCGTCATTCAAACATTGTTAAGTTGCTGTGCTGCATTTCTAGTGAGAATTCGAAGCTTCTGGTGTATGAATACATGGAGAATCATAGCCTGGATAAGTGGCTTCATGGAAAGAAGACAAGAATAATGGCGGGAATGACTTTGGCTCAACATGTTGTTTTGGACTGGCCAAAGAGACTAGAGATTGCAATAGGGGCAGCACAAGGCTTGTACTACATGCACCATGAGTGCTCTCCCCCCAGTAATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

392

Amino Acids

43.83

Weight (kDa)

9.12

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 37 - 139 1.1e-06 Leucine-rich repeat region
LRR_8 PF13855 90 - 126 1.1e-06 Leucine rich repeat
Pkinase PF00069 258 - 385 3e-16 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 259 - 384 1.7e-14 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 923
AccB7I CCANNNNNTGG 2 cut(s) 697, 730
AccII CGCG 1 cut(s) 845
AciI CCGC 2 cut(s) 845, 1058
AclWI GGATC 1 cut(s) 277
AcoI YGGCCR 2 cut(s) 688, 1094
AcsI RAATTY 6 cut(s) 86, 99, 374, 836, 896, 976
AcvI CACGTG 1 cut(s) 833
AfaI GTAC 2 cut(s) 820, 1139
AfiI CCNNNNNNNGG 3 cut(s) 355, 697, 730
AflIII ACRYGT 1 cut(s) 1078
AgsI TTSAA 6 cut(s) 438, 502, 510, 748, 901, 939
AjnI CCWGG 1 cut(s) 1017
AluBI AGCT 9 cut(s) 31, 50, 202, 304, 326, 445, 712, 886, 985
AluI AGCT 9 cut(s) 31, 50, 202, 304, 326, 445, 712, 886, 985
Alw21I GWGCWC 1 cut(s) 1160
Alw26I GTCTC 2 cut(s) 660, 1096
AlwI GGATC 1 cut(s) 277
Ama87I CYCGRG 1 cut(s) 887
AoxI GGCC 3 cut(s) 403, 688, 1094
ApeKI GCWGC 3 cut(s) 28, 960, 1124
ApoI RAATTY 6 cut(s) 86, 99, 374, 836, 896, 976
Asp700I GAANNNNTTC 1 cut(s) 896
AsuHPI GGTGA 2 cut(s) 107, 251
AsuII TTCGAA 2 cut(s) 53, 980
AsuNHI GCTAGC 1 cut(s) 46
AvaI CYCGRG 1 cut(s) 887
BaeGI GKGCMC 1 cut(s) 926
BalI TGGCCA 2 cut(s) 690, 1096
BanI GGYRCC 1 cut(s) 923
BbrPI CACGTG 1 cut(s) 833
BbsI GAAGAC 2 cut(s) 426, 1049
Bbv12I GWGCWC 1 cut(s) 1160
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 3 cut(s) 40, 947, 1136
BccI CCATC 2 cut(s) 215, 400
BciT130I CCWGG 1 cut(s) 1019
BcoDI GTCTC 2 cut(s) 660, 1096
BfaI CTAG 7 cut(s) 47, 63, 284, 632, 875, 969, 1106
BglII AGATCT 1 cut(s) 286
BisI GCNGC 3 cut(s) 29, 961, 1125
BlsI GCNGC 3 cut(s) 30, 962, 1126
BmcAI AGTACT 1 cut(s) 820
Bme1390I CCNGG 1 cut(s) 1019
BmeT110I CYCGRG 1 cut(s) 887
BmiI GGNNCC 1 cut(s) 925
BmrFI CCNGG 1 cut(s) 1019
BmrI ACTGGG 4 cut(s) 170, 540, 929, 1161
BmtI GCTAGC 1 cut(s) 50
BmuI ACTGGG 4 cut(s) 170, 540, 929, 1161
BpiI GAAGAC 2 cut(s) 426, 1049
BpmI CTGGAG 1 cut(s) 571
Bpu10I CCTNAGC 2 cut(s) 796, 906
Bpu14I TTCGAA 2 cut(s) 53, 980
BpuEI CTTGAG 2 cut(s) 277, 430
Bsa29I ATCGAT 1 cut(s) 229
BsaAI YACGTR 1 cut(s) 833
BsaBI GATNNNNATC 2 cut(s) 225, 774
BsaJI CCNNGG 1 cut(s) 35
BsaWI WCCGGW 1 cut(s) 672
Bsc4I CCNNNNNNNGG 3 cut(s) 355, 697, 730
Bse1I ACTGG 7 cut(s) 72, 104, 176, 546, 924, 1097, 1167
Bse3DI GCAATG 1 cut(s) 258
Bse8I GATNNNNATC 2 cut(s) 225, 774
BseBI CCWGG 1 cut(s) 1019
BseCI ATCGAT 1 cut(s) 229
BseDI CCNNGG 1 cut(s) 35
BseJI GATNNNNATC 2 cut(s) 225, 774
BseLI CCNNNNNNNGG 3 cut(s) 355, 697, 730
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 5 cut(s) 387, 393, 732, 810, 897
BseNI ACTGG 7 cut(s) 72, 104, 176, 546, 924, 1097, 1167
BseRI GAGGAG 1 cut(s) 338
BseSI GKGCMC 1 cut(s) 926
BseXI GCAGC 3 cut(s) 40, 947, 1136
Bsh1236I CGCG 1 cut(s) 845
BshFI GGCC 3 cut(s) 405, 690, 1096
BshNI GGYRCC 1 cut(s) 923
BshVI ATCGAT 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 1160
BsiHKCI CYCGRG 1 cut(s) 887
BsiSI CCGG 1 cut(s) 673
BslI CCNNNNNNNGG 3 cut(s) 355, 697, 730
BsmAI GTCTC 2 cut(s) 660, 1096
BsnI GGCC 3 cut(s) 405, 690, 1096
BsoBI CYCGRG 1 cut(s) 887
Bsp119I TTCGAA 2 cut(s) 53, 980
Bsp1286I GDGCHC 2 cut(s) 926, 1160
Bsp143I GATC 5 cut(s) 255, 269, 286, 603, 877
BspACI CCGC 2 cut(s) 845, 1058
BspANI GGCC 3 cut(s) 405, 690, 1096
BspCNI CTCAG 5 cut(s) 388, 392, 733, 809, 898
BspDI ATCGAT 1 cut(s) 229
BspFNI CGCG 1 cut(s) 845
BspLI GGNNCC 1 cut(s) 925
BspOI GCTAGC 1 cut(s) 50
BspPI GGATC 1 cut(s) 277
BspT104I TTCGAA 2 cut(s) 53, 980
BspT107I GGYRCC 1 cut(s) 923
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 7 cut(s) 72, 104, 176, 546, 924, 1097, 1167
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 5 cut(s) 255, 269, 286, 603, 877
Bst2UI CCWGG 1 cut(s) 1019
Bst4CI ACNGT 2 cut(s) 494, 640
BstBAI YACGTR 1 cut(s) 833
BstBI TTCGAA 2 cut(s) 53, 980
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 6 cut(s) 379, 396, 607, 741, 796, 906
BstFNI CGCG 1 cut(s) 845
BstKTI GATC 5 cut(s) 258, 272, 289, 606, 880
BstMAI GTCTC 2 cut(s) 660, 1096
BstMBI GATC 5 cut(s) 255, 269, 286, 603, 877
BstMWI GCNNNNNNNGC 2 cut(s) 25, 1121
BstNI CCWGG 1 cut(s) 1019
BstNSI RCATGY 2 cut(s) 1082, 1147
BstSCI CCNGG 1 cut(s) 1017
BstSLI GKGCMC 1 cut(s) 926
BstUI CGCG 1 cut(s) 845
BstV1I GCAGC 3 cut(s) 40, 947, 1136
BstV2I GAAGAC 2 cut(s) 426, 1049
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
Bsu15I ATCGAT 1 cut(s) 229
BsuRI GGCC 3 cut(s) 405, 690, 1096
BsuTUI ATCGAT 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 738
Cac8I GCNNGC 1 cut(s) 48
ClaI ATCGAT 1 cut(s) 229
Csp6I GTAC 2 cut(s) 819, 1138
CviAII CATG 8 cut(s) 601, 692, 705, 1003, 1034, 1079, 1144, 1151
CviQI GTAC 2 cut(s) 819, 1138
DdeI CTNAG 6 cut(s) 379, 396, 607, 741, 796, 906
DpnI GATC 5 cut(s) 257, 271, 288, 605, 879
DpnII GATC 5 cut(s) 255, 269, 286, 603, 877
EaeI YGGCCR 2 cut(s) 688, 1094
Eco72I CACGTG 1 cut(s) 833
Eco88I CYCGRG 1 cut(s) 887
EcoRI GAATTC 2 cut(s) 896, 976
EcoRII CCWGG 1 cut(s) 1017
FaeI CATG 8 cut(s) 604, 695, 708, 1006, 1037, 1082, 1147, 1154
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FatI CATG 8 cut(s) 600, 691, 704, 1002, 1033, 1078, 1143, 1150
FauI CCCGC 1 cut(s) 1051
Fnu4HI GCNGC 3 cut(s) 29, 961, 1125
Fsp4HI GCNGC 3 cut(s) 29, 961, 1125
FspBI CTAG 7 cut(s) 47, 63, 284, 632, 875, 969, 1106
GluI GCNGC 3 cut(s) 29, 961, 1125
GsuI CTGGAG 1 cut(s) 571
HaeIII GGCC 3 cut(s) 405, 690, 1096
HapII CCGG 1 cut(s) 673
Hin1II CATG 8 cut(s) 604, 695, 708, 1006, 1037, 1082, 1147, 1154
HincII GTYRAC 1 cut(s) 648
HindII GTYRAC 1 cut(s) 648
HindIII AAGCTT 2 cut(s) 710, 983
HinfI GANTC 6 cut(s) 55, 154, 226, 457, 591, 1009
HpaII CCGG 1 cut(s) 673
HphI GGTGA 2 cut(s) 107, 251
Hpy166II GTNNAC 2 cut(s) 109, 648
Hpy188I TCNGA 4 cut(s) 36, 397, 665, 882
Hpy8I GTNNAC 2 cut(s) 109, 648
HpyAV CCTTC 2 cut(s) 551, 796
HpyCH4III ACNGT 2 cut(s) 494, 640
HpyCH4IV ACGT 2 cut(s) 111, 832
HpyCH4V TGCA 3 cut(s) 963, 1115, 1147
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 1121
HpyF3I CTNAG 6 cut(s) 379, 396, 607, 741, 796, 906
HpySE526I ACGT 2 cut(s) 111, 832
Hsp92II CATG 8 cut(s) 604, 695, 708, 1006, 1037, 1082, 1147, 1154
Kzo9I GATC 5 cut(s) 255, 269, 286, 603, 877
Lsp1109I GCAGC 3 cut(s) 40, 947, 1136
MaeI CTAG 7 cut(s) 47, 63, 284, 632, 875, 969, 1106
MaeII ACGT 2 cut(s) 111, 832
MaeIII GTNAC 2 cut(s) 213, 634
MalI GATC 5 cut(s) 257, 271, 288, 605, 879
MboI GATC 5 cut(s) 255, 269, 286, 603, 877
MboII GAAGA 4 cut(s) 426, 466, 691, 1054
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 2 cut(s) 926, 1160
MlsI TGGCCA 2 cut(s) 690, 1096
MluNI TGGCCA 2 cut(s) 690, 1096
MlyI GAGTC 1 cut(s) 600
MmeI TCCRAC 2 cut(s) 344, 711
Mox20I TGGCCA 2 cut(s) 690, 1096
MroXI GAANNNNTTC 1 cut(s) 896
MscI TGGCCA 2 cut(s) 690, 1096
MseI TTAA 3 cut(s) 141, 849, 948
MslI CAYNNNNRTG 1 cut(s) 623
Msp20I TGGCCA 2 cut(s) 690, 1096
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 1 cut(s) 673
MspR9I CCNGG 1 cut(s) 1019
MvaI CCWGG 1 cut(s) 1019
MvnI CGCG 1 cut(s) 845
MwoI GCNNNNNNNGC 2 cut(s) 25, 1121
NdeII GATC 5 cut(s) 255, 269, 286, 603, 877
NheI GCTAGC 1 cut(s) 46
NlaIII CATG 8 cut(s) 604, 695, 708, 1006, 1037, 1082, 1147, 1154
NlaIV GGNNCC 1 cut(s) 925
NmuCI GTSAC 1 cut(s) 213
NspI RCATGY 2 cut(s) 1082, 1147
NspV TTCGAA 2 cut(s) 53, 980
PaeR7I CTCGAG 1 cut(s) 887
PciI ACATGT 1 cut(s) 1078
PdmI GAANNNNTTC 1 cut(s) 896
PfeI GAWTC 5 cut(s) 55, 154, 226, 457, 1009
PflMI CCANNNNNTGG 2 cut(s) 697, 730
PkrI GCNGC 3 cut(s) 30, 962, 1126
PleI GAGTC 1 cut(s) 599
PmaCI CACGTG 1 cut(s) 833
PmlI CACGTG 1 cut(s) 833
PpsI GAGTC 1 cut(s) 599
Ppu21I YACGTR 1 cut(s) 833
PscI ACATGT 1 cut(s) 1078
Psp6I CCWGG 1 cut(s) 1017
PspCI CACGTG 1 cut(s) 833
PspGI CCWGG 1 cut(s) 1017
PspN4I GGNNCC 1 cut(s) 925
PsuI RGATCY 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 202
RsaI GTAC 2 cut(s) 820, 1139
RsaNI GTAC 2 cut(s) 819, 1138
RseI CAYNNNNRTG 1 cut(s) 623
SaqAI TTAA 3 cut(s) 141, 849, 948
SatI GCNGC 3 cut(s) 29, 961, 1125
Sau3AI GATC 5 cut(s) 255, 269, 286, 603, 877
ScaI AGTACT 1 cut(s) 820
SchI GAGTC 1 cut(s) 600
ScrFI CCNGG 1 cut(s) 1019
SduI GDGCHC 2 cut(s) 926, 1160
Sfr274I CTCGAG 1 cut(s) 887
SfuI TTCGAA 2 cut(s) 53, 980
SlaI CTCGAG 1 cut(s) 887
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 3 cut(s) 292, 409, 887
SmoI CTYRAG 3 cut(s) 292, 409, 887
SsiI CCGC 2 cut(s) 845, 1058
SspMI CTAG 7 cut(s) 47, 63, 284, 632, 875, 969, 1106
StyD4I CCNGG 1 cut(s) 1017
TaaI ACNGT 2 cut(s) 494, 640
TaiI ACGT 2 cut(s) 114, 835
TaqI TCGA 6 cut(s) 53, 219, 229, 811, 888, 980
TatI WGTACW 2 cut(s) 818, 1137
TfiI GAWTC 5 cut(s) 55, 154, 226, 457, 1009
Tru1I TTAA 3 cut(s) 141, 849, 948
Tru9I TTAA 3 cut(s) 141, 849, 948
TscAI CASTG 1 cut(s) 745
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 3 cut(s) 28, 960, 1124
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 4 cut(s) 105, 1011, 1022, 1164
TspRI CASTG 1 cut(s) 745
Van91I CCANNNNNTGG 2 cut(s) 697, 730
XapI RAATTY 6 cut(s) 86, 99, 374, 836, 896, 976
XbaI TCTAGA 2 cut(s) 62, 283
XceI RCATGY 2 cut(s) 1082, 1147
XhoI CTCGAG 1 cut(s) 887
XmnI GAANNNNTTC 1 cut(s) 896
XspI CTAG 7 cut(s) 47, 63, 284, 632, 875, 969, 1106
ZrmI AGTACT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.