Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 422 |
| Acc36I | ACCTGC | 2 cut(s) | 422, 735 |
| AccB1I | GGYRCC | 1 cut(s) | 2961 |
| AccI | GTMKAC | 2 cut(s) | 1216, 2105 |
| AccII | CGCG | 2 cut(s) | 158, 3201 |
| AciI | CCGC | 9 cut(s) | 156, 158, 400, 695, 1193, 2229, 2304, 2672, 3132 |
| AclWI | GGATC | 3 cut(s) | 898, 1969, 2541 |
| AcoI | YGGCCR | 3 cut(s) | 1210, 1900, 3133 |
| AcuI | CTGAAG | 1 cut(s) | 1949 |
| AcyI | GRCGYC | 1 cut(s) | 2962 |
| AfaI | GTAC | 6 cut(s) | 7, 581, 1056, 1687, 2341, 2513 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 773, 1280, 2910 |
| AflIII | ACRYGT | 1 cut(s) | 2848 |
| AhlI | ACTAGT | 1 cut(s) | 1436 |
| AjnI | CCWGG | 4 cut(s) | 23, 395, 1640, 3139 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 3207, 3239 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1255, 1287 |
| Alw21I | GWGCWC | 5 cut(s) | 720, 912, 2839, 3044, 3178 |
| Alw26I | GTCTC | 3 cut(s) | 447, 809, 1837 |
| AlwI | GGATC | 3 cut(s) | 898, 1969, 2541 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1525, 3211 |
| ApeKI | GCWGC | 5 cut(s) | 36, 730, 935, 2542, 2633 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1288 |
| AspLEI | GCGC | 2 cut(s) | 2964, 3201 |
| AspS9I | GGNCC | 4 cut(s) | 1118, 1829, 1876, 2641 |
| AsuC2I | CCSGG | 2 cut(s) | 915, 1630 |
| AsuHPI | GGTGA | 8 cut(s) | 179, 403, 424, 454, 695, 844, 1952, 2218 |
| AsuII | TTCGAA | 1 cut(s) | 3194 |
| AvaII | GGWCC | 2 cut(s) | 1829, 1876 |
| AxyI | CCTNAGG | 1 cut(s) | 1695 |
| BalI | TGGCCA | 1 cut(s) | 1902 |
| BanI | GGYRCC | 1 cut(s) | 2961 |
| BanII | GRGCYC | 2 cut(s) | 720, 912 |
| BbsI | GAAGAC | 4 cut(s) | 878, 1844, 2982, 3222 |
| Bbv12I | GWGCWC | 5 cut(s) | 720, 912, 2839, 3044, 3178 |
| BbvCI | CCTCAGC | 2 cut(s) | 353, 2050 |
| BbvI | GCAGC | 5 cut(s) | 23, 742, 947, 2529, 2620 |
| BceAI | ACGGC | 4 cut(s) | 826, 1197, 1601, 2342 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 2336, 2370, 2921, 2955 |
| BciT130I | CCWGG | 4 cut(s) | 25, 397, 1642, 3141 |
| BclI | TGATCA | 1 cut(s) | 1738 |
| BcnI | CCSGG | 2 cut(s) | 915, 1630 |
| BcoDI | GTCTC | 3 cut(s) | 447, 809, 1837 |
| BcuI | ACTAGT | 1 cut(s) | 1436 |
| BfaI | CTAG | 6 cut(s) | 1437, 1488, 2043, 2574, 2753, 3090 |
| BfmI | CTRYAG | 2 cut(s) | 575, 728 |
| BfoI | RGCGCY | 1 cut(s) | 2965 |
| BfuAI | ACCTGC | 2 cut(s) | 422, 735 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2954 |
| BglII | AGATCT | 1 cut(s) | 2029 |
| BlpI | GCTNAGC | 1 cut(s) | 1934 |
| BmcAI | AGTACT | 3 cut(s) | 7, 581, 2513 |
| Bme1390I | CCNGG | 6 cut(s) | 25, 397, 915, 1630, 1642, 3141 |
| Bme18I | GGWCC | 2 cut(s) | 1829, 1876 |
| BmgT120I | GGNCC | 4 cut(s) | 1118, 1829, 1876, 2641 |
| BmiI | GGNNCC | 5 cut(s) | 725, 757, 1197, 2809, 2963 |
| BmrFI | CCNGG | 6 cut(s) | 25, 397, 915, 1630, 1642, 3141 |
| BmrI | ACTGGG | 2 cut(s) | 1125, 1413 |
| BmsI | GCATC | 7 cut(s) | 110, 1933, 2665, 2692, 2983, 3069, 3119 |
| BmuI | ACTGGG | 2 cut(s) | 1125, 1413 |
| BpiI | GAAGAC | 4 cut(s) | 878, 1844, 2982, 3222 |
| BpmI | CTGGAG | 4 cut(s) | 558, 1257, 2811, 2915 |
| Bpu10I | CCTNAGC | 5 cut(s) | 353, 719, 2050, 2308, 2637 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1934 |
| Bpu14I | TTCGAA | 1 cut(s) | 3194 |
| BpuEI | CTTGAG | 6 cut(s) | 51, 479, 1044, 1102, 1534, 3170 |
| BpuMI | CCSGG | 2 cut(s) | 915, 1630 |
| Bsa29I | ATCGAT | 1 cut(s) | 2536 |
| BsaAI | YACGTR | 1 cut(s) | 2849 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1743 |
| BsaHI | GRCGYC | 1 cut(s) | 2962 |
| BsaJI | CCNNGG | 6 cut(s) | 156, 641, 830, 913, 1629, 2481 |
| BsaWI | WCCGGW | 2 cut(s) | 434, 2906 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1255, 1285 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 773, 1280, 2910 |
| Bse118I | RCCGGY | 1 cut(s) | 691 |
| Bse21I | CCTNAGG | 1 cut(s) | 1695 |
| Bse3DI | GCAATG | 3 cut(s) | 287, 1265, 2820 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1743 |
| BseBI | CCWGG | 4 cut(s) | 25, 397, 1642, 3141 |
| BseCI | ATCGAT | 1 cut(s) | 2536 |
| BseDI | CCNNGG | 6 cut(s) | 156, 641, 830, 913, 1629, 2481 |
| BseGI | GGATG | 7 cut(s) | 33, 37, 55, 1637, 2561, 3037, 3136 |
| BseJI | GATNNNNATC | 1 cut(s) | 1743 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 773, 1280, 2910 |
| BseMI | GCAATG | 3 cut(s) | 287, 1265, 2820 |
| BseRI | GAGGAG | 3 cut(s) | 248, 251, 3168 |
| BseXI | GCAGC | 5 cut(s) | 23, 742, 947, 2529, 2620 |
| BsgI | GTGCAG | 4 cut(s) | 22, 400, 1854, 2528 |
| Bsh1236I | CGCG | 2 cut(s) | 158, 3201 |
| BshNI | GGYRCC | 1 cut(s) | 2961 |
| BshVI | ATCGAT | 1 cut(s) | 2536 |
| BsiHKAI | GWGCWC | 5 cut(s) | 720, 912, 2839, 3044, 3178 |
| BsiSI | CCGG | 5 cut(s) | 435, 692, 915, 1629, 2907 |
| BslFI | GGGAC | 1 cut(s) | 991 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 773, 1280, 2910 |
| BsmAI | GTCTC | 3 cut(s) | 447, 809, 1837 |
| BsmFI | GGGAC | 1 cut(s) | 991 |
| Bsp119I | TTCGAA | 1 cut(s) | 3194 |
| Bsp1286I | GDGCHC | 5 cut(s) | 720, 912, 2839, 3044, 3178 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1934 |
| Bsp19I | CCATGG | 1 cut(s) | 830 |
| BspACI | CCGC | 9 cut(s) | 156, 158, 400, 695, 1193, 2229, 2304, 2672, 3132 |
| BspDI | ATCGAT | 1 cut(s) | 2536 |
| BspFNI | CGCG | 2 cut(s) | 158, 3201 |
| BspHI | TCATGA | 1 cut(s) | 2659 |
| BspLI | GGNNCC | 5 cut(s) | 725, 757, 1197, 2809, 2963 |
| BspMAI | CTGCAG | 1 cut(s) | 732 |
| BspMI | ACCTGC | 2 cut(s) | 422, 735 |
| BspPI | GGATC | 3 cut(s) | 898, 1969, 2541 |
| BspQI | GCTCTTC | 2 cut(s) | 251, 1716 |
| BspT104I | TTCGAA | 1 cut(s) | 3194 |
| BspT107I | GGYRCC | 1 cut(s) | 2961 |
| BsrDI | GCAATG | 3 cut(s) | 287, 1265, 2820 |
| BsrFI | RCCGGY | 1 cut(s) | 691 |
| BssAI | RCCGGY | 1 cut(s) | 691 |
| BssECI | CCNNGG | 6 cut(s) | 156, 641, 830, 913, 1629, 2481 |
| BssNI | GRCGYC | 1 cut(s) | 2962 |
| BssT1I | CCWWGG | 2 cut(s) | 830, 2481 |
| Bst2UI | CCWGG | 4 cut(s) | 25, 397, 1642, 3141 |
| Bst4CI | ACNGT | 9 cut(s) | 348, 392, 566, 579, 968, 1233, 1685, 2666, 3221 |
| Bst6I | CTCTTC | 6 cut(s) | 251, 1034, 1716, 1957, 2189, 2385 |
| BstACI | GRCGYC | 1 cut(s) | 2962 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 3066, 3121 |
| BstBAI | YACGTR | 1 cut(s) | 2849 |
| BstBI | TTCGAA | 1 cut(s) | 3194 |
| BstC8I | GCNNGC | 5 cut(s) | 638, 1204, 1553, 2806, 3203 |
| BstDSI | CCRYGG | 2 cut(s) | 156, 830 |
| BstF5I | GGATG | 7 cut(s) | 33, 37, 55, 1637, 2561, 3037, 3136 |
| BstFNI | CGCG | 2 cut(s) | 158, 3201 |
| BstH2I | RGCGCY | 1 cut(s) | 2965 |
| BstHHI | GCGC | 2 cut(s) | 2964, 3201 |
| BstMAI | GTCTC | 3 cut(s) | 447, 809, 1837 |
| BstNI | CCWGG | 4 cut(s) | 25, 397, 1642, 3141 |
| BstNSI | RCATGY | 2 cut(s) | 1055, 2450 |
| BstSCI | CCNGG | 6 cut(s) | 23, 395, 913, 1628, 1640, 3139 |
| BstSFI | CTRYAG | 2 cut(s) | 575, 728 |
| BstUI | CGCG | 2 cut(s) | 158, 3201 |
| BstV1I | GCAGC | 5 cut(s) | 23, 742, 947, 2529, 2620 |
| BstV2I | GAAGAC | 4 cut(s) | 878, 1844, 2982, 3222 |
| BstX2I | RGATCY | 2 cut(s) | 1974, 2029 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 752, 1898, 2764 |
| BstYI | RGATCY | 2 cut(s) | 1974, 2029 |
| Bsu15I | ATCGAT | 1 cut(s) | 2536 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1695 |
| BsuTUI | ATCGAT | 1 cut(s) | 2536 |
| BtgI | CCRYGG | 2 cut(s) | 156, 830 |
| BtgZI | GCGATG | 1 cut(s) | 2361 |
| BtsCI | GGATG | 7 cut(s) | 33, 37, 55, 1637, 2561, 3037, 3136 |
| BtsI | GCAGTG | 3 cut(s) | 1704, 2631, 2937 |
| BtsIMutI | CAGTG | 8 cut(s) | 388, 938, 1329, 1401, 1704, 1871, 2631, 2937 |
| BveI | ACCTGC | 2 cut(s) | 422, 735 |
| Cac8I | GCNNGC | 5 cut(s) | 638, 1204, 1553, 2806, 3203 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1525, 3211 |
| CciI | TCATGA | 1 cut(s) | 2659 |
| CfoI | GCGC | 2 cut(s) | 2964, 3201 |
| Cfr10I | RCCGGY | 1 cut(s) | 691 |
| Cfr13I | GGNCC | 4 cut(s) | 1118, 1829, 1876, 2641 |
| Cfr42I | CCGCGG | 1 cut(s) | 159 |
| ClaI | ATCGAT | 1 cut(s) | 2536 |
| CseI | GACGC | 2 cut(s) | 226, 1670 |
| Csp6I | GTAC | 6 cut(s) | 6, 580, 1055, 1686, 2340, 2512 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 69, 104 |
| CviQI | GTAC | 6 cut(s) | 6, 580, 1055, 1686, 2340, 2512 |
| DinI | GGCGCC | 1 cut(s) | 2963 |
| DraI | TTTAAA | 2 cut(s) | 972, 1176 |
| EaeI | YGGCCR | 3 cut(s) | 1210, 1900, 3133 |
| Eam1104I | CTCTTC | 6 cut(s) | 251, 1034, 1716, 1957, 2189, 2385 |
| EarI | CTCTTC | 6 cut(s) | 251, 1034, 1716, 1957, 2189, 2385 |
| Ecl136II | GAGCTC | 2 cut(s) | 718, 910 |
| Eco130I | CCWWGG | 2 cut(s) | 830, 2481 |
| Eco147I | AGGCCT | 1 cut(s) | 3094 |
| Eco24I | GRGCYC | 2 cut(s) | 720, 912 |
| Eco32I | GATATC | 1 cut(s) | 628 |
| Eco47I | GGWCC | 2 cut(s) | 1829, 1876 |
| Eco53kI | GAGCTC | 2 cut(s) | 718, 910 |
| Eco57I | CTGAAG | 1 cut(s) | 1949 |
| Eco81I | CCTNAGG | 1 cut(s) | 1695 |
| EcoICRI | GAGCTC | 2 cut(s) | 718, 910 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2641 |
| EcoRI | GAATTC | 1 cut(s) | 3190 |
| EcoRII | CCWGG | 4 cut(s) | 23, 395, 1640, 3139 |
| EcoRV | GATATC | 1 cut(s) | 628 |
| EcoT14I | CCWWGG | 2 cut(s) | 830, 2481 |
| EcoT22I | ATGCAT | 1 cut(s) | 2658 |
| EcoT38I | GRGCYC | 2 cut(s) | 720, 912 |
| EgeI | GGCGCC | 1 cut(s) | 2963 |
| EheI | GGCGCC | 1 cut(s) | 2963 |
| ErhI | CCWWGG | 2 cut(s) | 830, 2481 |
| FaqI | GGGAC | 1 cut(s) | 991 |
| FauI | CCCGC | 1 cut(s) | 151 |
| FauNDI | CATATG | 1 cut(s) | 2066 |
| FbaI | TGATCA | 1 cut(s) | 1738 |
| FblI | GTMKAC | 2 cut(s) | 1216, 2105 |
| FokI | GGATG | 7 cut(s) | 40, 42, 44, 1624, 2548, 3044, 3123 |
| FriOI | GRGCYC | 2 cut(s) | 720, 912 |
| FspBI | CTAG | 6 cut(s) | 1437, 1488, 2043, 2574, 2753, 3090 |
| GlaI | GCGC | 2 cut(s) | 2963, 3200 |
| GsuI | CTGGAG | 4 cut(s) | 558, 1257, 2811, 2915 |
| HaeII | RGCGCY | 1 cut(s) | 2965 |
| HapII | CCGG | 5 cut(s) | 435, 692, 915, 1629, 2907 |
| HgaI | GACGC | 2 cut(s) | 226, 1670 |
| HhaI | GCGC | 2 cut(s) | 2964, 3201 |
| Hin1I | GRCGYC | 1 cut(s) | 2962 |
| Hin6I | GCGC | 2 cut(s) | 2962, 3199 |
| HinP1I | GCGC | 2 cut(s) | 2962, 3199 |
| HincII | GTYRAC | 4 cut(s) | 451, 1155, 1374, 2901 |
| HindII | GTYRAC | 4 cut(s) | 451, 1155, 1374, 2901 |
| HindIII | AAGCTT | 3 cut(s) | 552, 2011, 2396 |
| HinfI | GANTC | 8 cut(s) | 364, 616, 1516, 1576, 1744, 1787, 2521, 2717 |
| HpaI | GTTAAC | 1 cut(s) | 1155 |
| HpaII | CCGG | 5 cut(s) | 435, 692, 915, 1629, 2907 |
| HphI | GGTGA | 8 cut(s) | 179, 403, 424, 454, 695, 844, 1952, 2218 |
| Hpy166II | GTNNAC | 9 cut(s) | 451, 1155, 1217, 1374, 1500, 2106, 2584, 2901, 3148 |
| Hpy8I | GTNNAC | 9 cut(s) | 451, 1155, 1217, 1374, 1500, 2106, 2584, 2901, 3148 |
| Hpy99I | CGWCG | 1 cut(s) | 2933 |
| HpyCH4III | ACNGT | 9 cut(s) | 348, 392, 566, 579, 968, 1233, 1685, 2666, 3221 |
| HpyCH4IV | ACGT | 1 cut(s) | 2848 |
| HpySE526I | ACGT | 1 cut(s) | 2848 |
| Hsp92I | GRCGYC | 1 cut(s) | 2962 |
| HspAI | GCGC | 2 cut(s) | 2962, 3199 |
| KasI | GGCGCC | 1 cut(s) | 2961 |
| Ksp22I | TGATCA | 1 cut(s) | 1738 |
| KspAI | GTTAAC | 1 cut(s) | 1155 |
| KspI | CCGCGG | 1 cut(s) | 159 |
| LguI | GCTCTTC | 2 cut(s) | 251, 1716 |
| LmnI | GCTCC | 6 cut(s) | 915, 2813, 2830, 3047, 3129, 3181 |
| Lsp1109I | GCAGC | 5 cut(s) | 23, 742, 947, 2529, 2620 |
| LweI | GCATC | 7 cut(s) | 110, 1933, 2665, 2692, 2983, 3069, 3119 |
| MaeI | CTAG | 6 cut(s) | 1437, 1488, 2043, 2574, 2753, 3090 |
| MaeII | ACGT | 1 cut(s) | 2848 |
| MaeIII | GTNAC | 7 cut(s) | 430, 683, 708, 776, 1410, 1616, 2702 |
| MfeI | CAATTG | 1 cut(s) | 1980 |
| MflI | RGATCY | 2 cut(s) | 1974, 2029 |
| MhlI | GDGCHC | 5 cut(s) | 720, 912, 2839, 3044, 3178 |
| MlsI | TGGCCA | 1 cut(s) | 1902 |
| MluNI | TGGCCA | 1 cut(s) | 1902 |
| Mly113I | GGCGCC | 1 cut(s) | 2962 |
| MlyI | GAGTC | 2 cut(s) | 1525, 2711 |
| MmeI | TCCRAC | 1 cut(s) | 1649 |
| Mox20I | TGGCCA | 1 cut(s) | 1902 |
| Mph1103I | ATGCAT | 1 cut(s) | 2658 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1288 |
| MscI | TGGCCA | 1 cut(s) | 1902 |
| MslI | CAYNNNNRTG | 4 cut(s) | 750, 885, 2664, 2688 |
| Msp20I | TGGCCA | 1 cut(s) | 1902 |
| MspA1I | CMGCKG | 2 cut(s) | 158, 733 |
| MspI | CCGG | 5 cut(s) | 435, 692, 915, 1629, 2907 |
| MspR9I | CCNGG | 6 cut(s) | 25, 397, 915, 1630, 1642, 3141 |
| MunI | CAATTG | 1 cut(s) | 1980 |
| MvaI | CCWGG | 4 cut(s) | 25, 397, 1642, 3141 |
| MvnI | CGCG | 2 cut(s) | 158, 3201 |
| NarI | GGCGCC | 1 cut(s) | 2962 |
| NciI | CCSGG | 2 cut(s) | 915, 1630 |
| NcoI | CCATGG | 1 cut(s) | 830 |
| NdeI | CATATG | 1 cut(s) | 2066 |
| NlaIV | GGNNCC | 5 cut(s) | 725, 757, 1197, 2809, 2963 |
| NmeAIII | GCCGAG | 1 cut(s) | 622 |
| NmuCI | GTSAC | 2 cut(s) | 430, 683 |
| NsiI | ATGCAT | 1 cut(s) | 2658 |
| NspI | RCATGY | 2 cut(s) | 1055, 2450 |
| NspV | TTCGAA | 1 cut(s) | 3194 |
| PagI | TCATGA | 1 cut(s) | 2659 |
| PaqCI | CACCTGC | 1 cut(s) | 422 |
| PceI | AGGCCT | 1 cut(s) | 3094 |
| PciSI | GCTCTTC | 2 cut(s) | 251, 1716 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1288 |
| PfeI | GAWTC | 6 cut(s) | 364, 616, 1576, 1744, 1787, 2521 |
| PflFI | GACNNNGTC | 1 cut(s) | 1848 |
| PfoI | TCCNGGA | 2 cut(s) | 1640, 3139 |
| PleI | GAGTC | 2 cut(s) | 1524, 2711 |
| PluTI | GGCGCC | 1 cut(s) | 2965 |
| PpsI | GAGTC | 2 cut(s) | 1524, 2711 |
| Ppu21I | YACGTR | 1 cut(s) | 2849 |
| Psp124BI | GAGCTC | 2 cut(s) | 720, 912 |
| Psp6I | CCWGG | 4 cut(s) | 23, 395, 1640, 3139 |
| PspGI | CCWGG | 4 cut(s) | 23, 395, 1640, 3139 |
| PspN4I | GGNNCC | 5 cut(s) | 725, 757, 1197, 2809, 2963 |
| PspPI | GGNCC | 4 cut(s) | 1118, 1829, 1876, 2641 |
| PstI | CTGCAG | 1 cut(s) | 732 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1525, 3211 |
| PsuI | RGATCY | 2 cut(s) | 1974, 2029 |
| PsyI | GACNNNGTC | 1 cut(s) | 1848 |
| PvuII | CAGCTG | 1 cut(s) | 733 |
| RsaI | GTAC | 6 cut(s) | 7, 581, 1056, 1687, 2341, 2513 |
| RsaNI | GTAC | 6 cut(s) | 6, 580, 1055, 1686, 2340, 2512 |
| RseI | CAYNNNNRTG | 4 cut(s) | 750, 885, 2664, 2688 |
| SacI | GAGCTC | 2 cut(s) | 720, 912 |
| SacII | CCGCGG | 1 cut(s) | 159 |
| SapI | GCTCTTC | 2 cut(s) | 251, 1716 |
| Sau96I | GGNCC | 4 cut(s) | 1118, 1829, 1876, 2641 |
| ScaI | AGTACT | 3 cut(s) | 7, 581, 2513 |
| SchI | GAGTC | 2 cut(s) | 1525, 2711 |
| ScrFI | CCNGG | 6 cut(s) | 25, 397, 915, 1630, 1642, 3141 |
| SduI | GDGCHC | 5 cut(s) | 720, 912, 2839, 3044, 3178 |
| SfaNI | GCATC | 7 cut(s) | 110, 1933, 2665, 2692, 2983, 3069, 3119 |
| SfcI | CTRYAG | 2 cut(s) | 575, 728 |
| SfoI | GGCGCC | 1 cut(s) | 2963 |
| Sfr303I | CCGCGG | 1 cut(s) | 159 |
| SfuI | TTCGAA | 1 cut(s) | 3194 |
| SgrBI | CCGCGG | 1 cut(s) | 159 |
| SinI | GGWCC | 2 cut(s) | 1829, 1876 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 750, 885, 2664, 2688 |
| SmlI | CTYRAG | 6 cut(s) | 66, 494, 1059, 1081, 1513, 3185 |
| SmoI | CTYRAG | 6 cut(s) | 66, 494, 1059, 1081, 1513, 3185 |
| SpeI | ACTAGT | 1 cut(s) | 1436 |
| SseBI | AGGCCT | 1 cut(s) | 3094 |
| SsiI | CCGC | 9 cut(s) | 156, 158, 400, 695, 1193, 2229, 2304, 2672, 3132 |
| SspDI | GGCGCC | 1 cut(s) | 2961 |
| SspMI | CTAG | 6 cut(s) | 1437, 1488, 2043, 2574, 2753, 3090 |
| SstI | GAGCTC | 2 cut(s) | 720, 912 |
| StuI | AGGCCT | 1 cut(s) | 3094 |
| StyD4I | CCNGG | 6 cut(s) | 23, 395, 913, 1628, 1640, 3139 |
| StyI | CCWWGG | 2 cut(s) | 830, 2481 |
| TaaI | ACNGT | 9 cut(s) | 348, 392, 566, 579, 968, 1233, 1685, 2666, 3221 |
| TaiI | ACGT | 1 cut(s) | 2851 |
| TatI | WGTACW | 3 cut(s) | 5, 579, 2511 |
| TauI | GCSGC | 6 cut(s) | 158, 403, 698, 2307, 2674, 3135 |
| TfiI | GAWTC | 6 cut(s) | 364, 616, 1576, 1744, 1787, 2521 |
| TscAI | CASTG | 8 cut(s) | 395, 945, 1336, 1408, 1711, 1878, 2631, 2944 |
| TseFI | GTSAC | 2 cut(s) | 430, 683 |
| TseI | GCWGC | 5 cut(s) | 36, 730, 935, 2542, 2633 |
| Tsp45I | GTSAC | 2 cut(s) | 430, 683 |
| TspDTI | ATGAA | 8 cut(s) | 140, 470, 472, 755, 1234, 2164, 3048, 3116 |
| TspGWI | ACGGA | 3 cut(s) | 748, 2022, 3034 |
| TspRI | CASTG | 8 cut(s) | 395, 945, 1336, 1408, 1711, 1878, 2631, 2944 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1848 |
| VpaK11BI | GGWCC | 2 cut(s) | 1829, 1876 |
| XceI | RCATGY | 2 cut(s) | 1055, 2450 |
| XmiI | GTMKAC | 2 cut(s) | 1216, 2105 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1288 |
| XspI | CTAG | 6 cut(s) | 1437, 1488, 2043, 2574, 2753, 3090 |
| ZrmI | AGTACT | 3 cut(s) | 7, 581, 2513 |
| Zsp2I | ATGCAT | 1 cut(s) | 2658 |