RchiOBHm_Chr2g0091961
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001321
Physical Location & Seq
Forward (+)
5579199 .. 5583785
4587 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46713

Sequence Viewer

Length: 3135 bp
ATGGTTCCAAGTTCTAACACAGCTCTGAAACAGTTAAGCCTCCATATCCAAATGACTACACTAACCCCGAAACCTTCTCTCATTTTCCGCACCTTCTTCATCTTCTTCATCCATGCAAACTCTCAGTCCCTTCAGGACAAGGAATTATCAGTCCTGCTTAAATTAAAGTCATATTGGCTACCTCCACCAGCTTTCAGTCATTGGACTTCATCATCAGACTCTGGTTCCCACTGTAACTGGCCTGAGATCAACTGCACCGGAAACTCCGTCACCGGATTGAATCTCAACACCGTAAACCTCAGCCTTCAAGTTCCACCCTTCATTTGTGACCTCAACAACCTCACACACCTTGACCTTGGTAATAACTACCTCCCGGGAGGTTTTCCTTCGTCTCTCTACGACTGCTCCAAGCTAGAGTATCTAGACCTCTCGCAAAACTACTTTGTGGGTACAATTCCTAATGGCATCGACGGATTGCCTAAGCTTAAGATCCTCAACCTCGCCGGTAACAACTTCTCCGGTGACATTCCGGCGTCTATTGGGAACTTACAGGAGCTGGCTACTTTGGATATATACATGAACCAGCTCAACGGCTCTGTCCCTGCTGAAATAGGTGACCTCTCCAATCTTGAGCAACTCTGGCTCTCTTGGCTTCCCAAAATGGCGCCATGGAAGCTGCCTTTTAACTTCACCAAGTTGAAAAAGATGAAGATATTGAAGATACGCGAAGCAAATTTGATTGGGGAAATACCAGAATCTATTGGAGAACTGGAAGCACTTGAAGTATTGGATTTGGCGCAGAACAAGTTGAGCGGGAATATACCAAGCGGTGTGTTTCTGTTCAAGAACTTGAGCATTATATATCTGTTCAAGAACAGGCTTTCAGGGGAGATTCCTCAAGTGGTTGAAGCACTCAACTTGAGCATCGTTGACATCTCAGAGAACACTCTCACAGGGCCGATACCACAAGGTTTCGGAAACCTTACCAAGTTGACAGATATGAGTTTGTTCTACAATGGTATTTCAGGTGAGATTCCAGAGAGCATTGGCCGCCTGCCAAATCTTGTGATTTTCAAAATGTTCAACATGAACTTGTCAGGAACAATTCCTCCAGAGCTTGGAAAGCATTCGCTGCTTGAAGATTTCCAGGTTTCGGTGAATAGGCTTACCGGAAAGCTGCCAGACGGCTTGTGTAAAAATGGTAAGCTTGTGGCAGTTGTTGCTTATGAGAATAGTCTCACTGGGGAGCTACCAAGCTCTCTTGGAAATTGTGATAGTCTGCTGGTAGTCAATGTCTATGATAACGTGCTATCTGGGGACATTCCTAGTGGTATGTGGAATGCATTGAATTTGAGTTATGTGATGATGAGCAACAATTCGTTCACGGGTGAGCTTCCTGAGAAGATGTCATATAATCTTCAAAGGCTGGAAATCGGAGATAACAAGTTTTCAGGTCAGATTCCAAGAGGGGTGTCTTCCTGTAAGAATCTGCAAGTTTTTGATGCTGGTAACAACCTCTTCAATGGTAGTATTCCTCAGGAATTAACTACTCTTCCGAGCTTGACGACTCTGACCCTGCATCAGAATCAGCTTAGCGGGCCCCTTCCGTCAGATATTGTATCATGGAAGTCACTAAACACTCTTGATCTGCGTAGAAATCAGCTCTCCGGACCAATTCCAGAGATACTTGGTGGTTTGCCCGCCCTTACTGAATTGGACCTTTCTGAAAACCAGTTTTCAGGCCAAATTCCAAATCAACTTGGCCATCTGAAACTCAATGAGTTCAACCTCTCTTCAAATCTCCTCTCTGGCGAGATCCCAATTTCATTTGAAAACTCTGCGTATGAGAGAAGCTTCTTGAACAACACCGGTCTCTGTGCAAGCACCAGTGGTGTGAATCTCAATATATGCTCTCAATCGCCAAAGTCCAGCAAGATTTCAATTCGGTCTCTTGCTTTGATCTTAAGTCTAAGCGCGGTGTTGTTCGTGTTGGTTTTGTCCTTATTAATCTTCTTTGTTAGAGGTTACTTGAGGAGGAAGGATGGATCAGACGCAGACTGGAAGCTCGTTGCATTTCAGAGGTTGAATTTCACCATCTCACAAATCTTGTCAGGGTTGATAGAAAGCAATGTGATTGGAAGTGGTGGATCAGGGAGGGTCTATCGTGTACCTGTGAATCGTACAGGTGATGTTGTAGCTGCGAAGAGGATTTGGACCAACAAGAAGATCATGGAGGAGAGGCTTGAAAAAGAGTTTCATGCAGAAGTCAAGATACTGAGCTCAATTCGACATGCTAACATAGTGAAGCTAATGTGCTGCATATCCAGTGAGACTTCGAAACTTCTTGTATATGAGTACTTAGACAATCGAAGCCTAGATCGATGGTTGCACAAGAAAAACAGGCCATACCTATCCAACCTCTCAAGTTCAGTCCATCATGTTGTTCTGGACTGGCCTAAGAGGTTGCAGATTGCAGTGGGAGCTGCTAAGGGCCTCTGCTATATGCATCATGACTGTGTTCAACCCGTTGTGCATCGAGACGTGAAATCAAGTAACATTTTATTGGACTCTGATTTCAACGCAAAAATAGCAGATTTTGGTCTGGCCAAGATGTTGGTCAAGCAAGGAGACCTTGCCACAATGTCAGCTGTTGCTGGTTCCTTTGGCTACATGGCTCCAGAATATGCACATACCAGAAAAGTGAATGAAAAGATCGATGTATATAGTTTCGGGGTCATCCTGCTGGAGTTGGCAACTGGTAAGGAACCCAATGAAGGTGATGAAAACACTTCTCTCGCCGAATGGGCGTGGCGTCATGCTCAAGAAGGCAAACCTATCGACAGTGTTCTGGACCAGGACGTCAAAGAACCCTGTTACTTGGATGAAATGTGCGCTGTTTTCAGACTCGGCATCATGTGCACCGAGACACTTCCTACTAATAGGCCTTCTATGAAAGAGGTTTTGCAGATCTTGGTTCGAACTAGTCCTCCTCCAGTTCGCAGGGAAAAGACTGAATATGCCGCTGCTCCTCTCCTCAAGAACTCGAGGCGAGAGAGAGTTTTGGAAGATCAAGATGGTTTGGCAACGAATCCCGAAGTGGACACCAATAAAAGGGGAAATAAGATGTGTATGTAG

Protein Analysis

1044

Amino Acids

115.47

Weight (kDa)

6.27

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 113 - 172 4.4e-11 Leucine rich repeat
LRR_14 PF23598 136 - 241 4.2e-09 Leucine-rich repeat region
LRR_4 PF12799 137 - 177 4.7e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 231 - 303 1.9e-06 Leucine-rich repeat region
LRR_8 PF13855 544 - 602 6.6e-07 Leucine rich repeat
Pkinase PF00069 709 - 989 1.1e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 709 - 990 3e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2858
AatII GACGTC 1 cut(s) 2862
AccB1I GGYRCC 1 cut(s) 664
AccB7I CCANNNNNTGG 1 cut(s) 1118
AccBSI CCGCTC 1 cut(s) 813
AccII CGCG 2 cut(s) 726, 1976
AccIII TCCGGA 1 cut(s) 1667
AciI CCGC 8 cut(s) 88, 813, 828, 1051, 1596, 1701, 1976, 3021
AclWI GGATC 4 cut(s) 484, 1810, 2053, 2155
AcoI YGGCCR 3 cut(s) 1048, 1762, 2604
AcsI RAATTY 4 cut(s) 733, 1348, 1746, 2086
AcuI CTGAAG 1 cut(s) 116
AcyI GRCGYC 4 cut(s) 533, 665, 2812, 2859
AfaI GTAC 4 cut(s) 451, 2169, 2182, 2357
AfiI CCNNNNNNNGG 4 cut(s) 1118, 1153, 2774, 3111
AflII CTTAAG 2 cut(s) 485, 1963
AgeI ACCGGT 1 cut(s) 1868
AhlI ACTAGT 1 cut(s) 2981
AjiI CACGTC 1 cut(s) 2542
AjnI CCWGG 2 cut(s) 1146, 2853
AloI GAACNNNNNNTCC 4 cut(s) 1093, 1125, 2971, 3003
Alw21I GWGCWC 2 cut(s) 2282, 2921
Alw26I GTCTC 8 cut(s) 396, 1241, 1877, 1953, 2324, 2532, 2622, 2918
Alw44I GTGCAC 1 cut(s) 2917
AlwI GGATC 4 cut(s) 484, 1810, 2053, 2155
AlwNI CAGNNNCTG 2 cut(s) 221, 556
Ama87I CYCGRG 2 cut(s) 373, 3043
Aor13HI TCCGGA 1 cut(s) 1667
ApaI GGGCCC 1 cut(s) 1602
ApaLI GTGCAC 1 cut(s) 2917
ApeKI GCWGC 7 cut(s) 676, 1132, 1177, 2198, 2316, 2483, 3023
ApoI RAATTY 4 cut(s) 733, 1348, 1746, 2086
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AseI ATTAAT 1 cut(s) 2006
AsiGI ACCGGT 1 cut(s) 1868
Asp700I GAANNNNTTC 1 cut(s) 1126
AspLEI GCGC 4 cut(s) 667, 799, 1976, 2894
AspS9I GGNCC 8 cut(s) 956, 1598, 1599, 1670, 1717, 2214, 2491, 2851
AsuC2I CCSGG 2 cut(s) 374, 375
AsuII TTCGAA 2 cut(s) 2336, 2977
AvaI CYCGRG 2 cut(s) 373, 3043
AvaII GGWCC 4 cut(s) 1670, 1717, 2214, 2851
AxyI CCTNAGG 1 cut(s) 1536
BaeGI GKGCMC 2 cut(s) 1602, 2921
BalI TGGCCA 2 cut(s) 1764, 2606
BanI GGYRCC 1 cut(s) 664
BanII GRGCYC 2 cut(s) 1602, 2282
BarI GAAGNNNNNNTAC 2 cut(s) 2256, 2288
BbsI GAAGAC 1 cut(s) 1467
Bbv12I GWGCWC 2 cut(s) 2282, 2921
BbvCI CCTCAGC 1 cut(s) 299
BbvI GCAGC 7 cut(s) 663, 1119, 1164, 2185, 2303, 2470, 3010
BccI CCATC 6 cut(s) 1773, 2036, 2102, 2376, 2442, 3068
BceAI ACGGC 2 cut(s) 607, 1201
BcgI CGANNNNNNTGC 2 cut(s) 2818, 2852
BciT130I CCWGG 2 cut(s) 1148, 2855
BcnI CCSGG 2 cut(s) 374, 375
BcoDI GTCTC 8 cut(s) 396, 1241, 1877, 1953, 2324, 2532, 2622, 2918
BcuI ACTAGT 1 cut(s) 2981
BfaI CTAG 5 cut(s) 413, 422, 1326, 2375, 2982
BfoI RGCGCY 1 cut(s) 668
BfrI CTTAAG 2 cut(s) 485, 1963
BglI GCCNNNNNGGC 1 cut(s) 2804
BglII AGATCT 1 cut(s) 2967
BisI GCNGC 9 cut(s) 677, 1051, 1133, 1178, 2199, 2317, 2484, 3021, 3024
BlpI GCTNAGC 1 cut(s) 1592
BlsI GCNGC 9 cut(s) 678, 1052, 1134, 1179, 2200, 2318, 2485, 3022, 3025
BmcAI AGTACT 1 cut(s) 2357
Bme1390I CCNGG 4 cut(s) 374, 375, 1148, 2855
Bme18I GGWCC 4 cut(s) 1670, 1717, 2214, 2851
BmeT110I CYCGRG 2 cut(s) 373, 3043
BmgBI CACGTC 1 cut(s) 2542
BmgT120I GGNCC 8 cut(s) 956, 1598, 1599, 1670, 1717, 2214, 2491, 2851
BmiI GGNNCC 8 cut(s) 6, 226, 666, 1600, 1601, 2659, 2676, 2766
BmrFI CCNGG 4 cut(s) 374, 375, 1148, 2855
BmrI ACTGGG 1 cut(s) 1251
BmsI GCATC 7 cut(s) 474, 933, 1492, 1588, 2515, 2542, 2919
BmuI ACTGGG 1 cut(s) 1251
BpiI GAAGAC 1 cut(s) 1467
BpmI CTGGAG 4 cut(s) 1095, 2661, 2765, 2976
Bpu10I CCTNAGC 3 cut(s) 299, 480, 2487
Bpu1102I GCTNAGC 1 cut(s) 1592
Bpu14I TTCGAA 2 cut(s) 2336, 2977
BpuEI CTTGAG 8 cut(s) 650, 871, 882, 940, 2050, 2407, 2805, 3020
BpuMI CCSGG 2 cut(s) 374, 375
Bsa29I ATCGAT 2 cut(s) 2380, 2715
BsaHI GRCGYC 4 cut(s) 533, 665, 2812, 2859
BsaI GGTCTC 3 cut(s) 1877, 1953, 2622
BsaJI CCNNGG 3 cut(s) 355, 373, 668
BsaWI WCCGGW 6 cut(s) 257, 272, 518, 1169, 1667, 1868
BsaXI ACNNNNNCTCC 8 cut(s) 389, 419, 500, 530, 1093, 1123, 2971, 3001
Bsc4I CCNNNNNNNGG 4 cut(s) 1118, 1153, 2774, 3111
Bse118I RCCGGY 2 cut(s) 503, 1868
Bse21I CCTNAGG 1 cut(s) 1536
Bse3DI GCAATG 1 cut(s) 2134
BseAI TCCGGA 1 cut(s) 1667
BseBI CCWGG 2 cut(s) 1148, 2855
BseCI ATCGAT 2 cut(s) 2380, 2715
BseDI CCNNGG 3 cut(s) 355, 373, 668
BseGI GGATG 4 cut(s) 108, 2047, 2736, 2887
BseLI CCNNNNNNNGG 4 cut(s) 1118, 1153, 2774, 3111
BseMI GCAATG 1 cut(s) 2134
BseMII CTCAG 7 cut(s) 137, 234, 313, 951, 1389, 1550, 2267
BseRI GAGGAG 6 cut(s) 1793, 2047, 2249, 2979, 3018, 3023
BseSI GKGCMC 2 cut(s) 1602, 2921
BseXI GCAGC 7 cut(s) 663, 1119, 1164, 2185, 2303, 2470, 3010
BsgI GTGCAG 1 cut(s) 238
Bsh1236I CGCG 2 cut(s) 726, 1976
BshNI GGYRCC 1 cut(s) 664
BshTI ACCGGT 1 cut(s) 1868
BshVI ATCGAT 2 cut(s) 2380, 2715
BsiHKAI GWGCWC 2 cut(s) 2282, 2921
BsiHKCI CYCGRG 2 cut(s) 373, 3043
BsiSI CCGG 9 cut(s) 258, 273, 374, 504, 519, 530, 1170, 1668, 1869
BslFI GGGAC 3 cut(s) 112, 584, 1331
BslI CCNNNNNNNGG 4 cut(s) 1118, 1153, 2774, 3111
BsmAI GTCTC 8 cut(s) 396, 1241, 1877, 1953, 2324, 2532, 2622, 2918
BsmBI CGTCTC 2 cut(s) 396, 2532
BsmFI GGGAC 3 cut(s) 112, 584, 1331
BsmI GAATGC 2 cut(s) 1126, 1345
Bso31I GGTCTC 3 cut(s) 1877, 1953, 2622
BsoBI CYCGRG 2 cut(s) 373, 3043
Bsp119I TTCGAA 2 cut(s) 2336, 2977
Bsp120I GGGCCC 1 cut(s) 1598
Bsp1286I GDGCHC 3 cut(s) 1602, 2282, 2921
Bsp13I TCCGGA 1 cut(s) 1667
Bsp1720I GCTNAGC 1 cut(s) 1592
Bsp19I CCATGG 1 cut(s) 668
BspACI CCGC 8 cut(s) 88, 813, 828, 1051, 1596, 1701, 1976, 3021
BspCNI CTCAG 7 cut(s) 136, 235, 312, 950, 1390, 1549, 2268
BspDI ATCGAT 2 cut(s) 2380, 2715
BspEI TCCGGA 1 cut(s) 1667
BspFNI CGCG 2 cut(s) 726, 1976
BspHI TCATGA 1 cut(s) 2509
BspLI GGNNCC 8 cut(s) 6, 226, 666, 1600, 1601, 2659, 2676, 2766
BspPI GGATC 4 cut(s) 484, 1810, 2053, 2155
BspT104I TTCGAA 2 cut(s) 2336, 2977
BspT107I GGYRCC 1 cut(s) 664
BspTI CTTAAG 2 cut(s) 485, 1963
BspTNI GGTCTC 3 cut(s) 1877, 1953, 2622
BsrBI CCGCTC 1 cut(s) 813
BsrDI GCAATG 1 cut(s) 2134
BsrFI RCCGGY 2 cut(s) 503, 1868
BssAI RCCGGY 2 cut(s) 503, 1868
BssECI CCNNGG 3 cut(s) 355, 373, 668
BssNI GRCGYC 4 cut(s) 533, 665, 2812, 2859
BssT1I CCWWGG 2 cut(s) 355, 668
Bst2UI CCWGG 2 cut(s) 1148, 2855
Bst4CI ACNGT 5 cut(s) 33, 233, 292, 2516, 2843
Bst6I CTCTTC 4 cut(s) 1523, 1557, 1798, 2198
BstACI GRCGYC 4 cut(s) 533, 665, 2812, 2859
BstAFI CTTAAG 2 cut(s) 485, 1963
BstAPI GCANNNNNTGC 3 cut(s) 1132, 1220, 2916
BstBI TTCGAA 2 cut(s) 2336, 2977
BstC8I GCNNGC 5 cut(s) 558, 1055, 1598, 1701, 1882
BstDSI CCRYGG 1 cut(s) 668
BstEII GGTNACC 1 cut(s) 614
BstF5I GGATG 4 cut(s) 108, 2047, 2736, 2887
BstFNI CGCG 2 cut(s) 726, 1976
BstH2I RGCGCY 1 cut(s) 668
BstHHI GCGC 4 cut(s) 667, 799, 1976, 2894
BstMAI GTCTC 8 cut(s) 396, 1241, 1877, 1953, 2324, 2532, 2622, 2918
BstNI CCWGG 2 cut(s) 1148, 2855
BstNSI RCATGY 1 cut(s) 2294
BstPI GGTNACC 1 cut(s) 614
BstSCI CCNGG 4 cut(s) 372, 373, 1146, 2853
BstSLI GKGCMC 2 cut(s) 1602, 2921
BstUI CGCG 2 cut(s) 726, 1976
BstV1I GCAGC 7 cut(s) 663, 1119, 1164, 2185, 2303, 2470, 3010
BstV2I GAAGAC 1 cut(s) 1467
BstX2I RGATCY 3 cut(s) 489, 1815, 2967
BstXI CCANNNNNNTGG 1 cut(s) 2614
BstYI RGATCY 3 cut(s) 489, 1815, 2967
Bsu15I ATCGAT 2 cut(s) 2380, 2715
Bsu36I CCTNAGG 1 cut(s) 1536
BsuTUI ATCGAT 2 cut(s) 2380, 2715
BtgI CCRYGG 1 cut(s) 668
BtrI CACGTC 1 cut(s) 2542
BtsCI GGATG 4 cut(s) 108, 2047, 2736, 2887
BtsI GCAGTG 1 cut(s) 2481
BtsIMutI CAGTG 6 cut(s) 229, 1239, 1894, 2332, 2481, 2848
Cac8I GCNNGC 5 cut(s) 558, 1055, 1598, 1701, 1882
CaiI CAGNNNCTG 2 cut(s) 221, 556
CciI TCATGA 1 cut(s) 2509
CfoI GCGC 4 cut(s) 667, 799, 1976, 2894
Cfr10I RCCGGY 2 cut(s) 503, 1868
Cfr13I GGNCC 8 cut(s) 956, 1598, 1599, 1670, 1717, 2214, 2491, 2851
Cfr9I CCCGGG 1 cut(s) 373
ClaI ATCGAT 2 cut(s) 2380, 2715
CseI GACGC 3 cut(s) 522, 2060, 2801
Csp6I GTAC 4 cut(s) 450, 2168, 2181, 2356
CspAI ACCGGT 1 cut(s) 1868
CviQI GTAC 4 cut(s) 450, 2168, 2181, 2356
DinI GGCGCC 1 cut(s) 666
DrdI GACNNNNNNGTC 1 cut(s) 2858
DseDI GACNNNNNNGTC 1 cut(s) 2858
EaeI YGGCCR 3 cut(s) 1048, 1762, 2604
Eam1104I CTCTTC 4 cut(s) 1523, 1557, 1798, 2198
EarI CTCTTC 4 cut(s) 1523, 1557, 1798, 2198
Ecl136II GAGCTC 1 cut(s) 2280
Eco130I CCWWGG 2 cut(s) 355, 668
Eco147I AGGCCT 1 cut(s) 2944
Eco24I GRGCYC 2 cut(s) 1602, 2282
Eco31I GGTCTC 3 cut(s) 1877, 1953, 2622
Eco47I GGWCC 4 cut(s) 1670, 1717, 2214, 2851
Eco53kI GAGCTC 1 cut(s) 2280
Eco57I CTGAAG 1 cut(s) 116
Eco81I CCTNAGG 1 cut(s) 1536
Eco88I CYCGRG 2 cut(s) 373, 3043
Eco91I GGTNACC 1 cut(s) 614
EcoICRI GAGCTC 1 cut(s) 2280
EcoO109I RGGNCCY 2 cut(s) 1599, 2491
EcoO65I GGTNACC 1 cut(s) 614
EcoRII CCWGG 2 cut(s) 1146, 2853
EcoT14I CCWWGG 2 cut(s) 355, 668
EcoT22I ATGCAT 2 cut(s) 1345, 2508
EcoT38I GRGCYC 2 cut(s) 1602, 2282
EgeI GGCGCC 1 cut(s) 666
EheI GGCGCC 1 cut(s) 666
ErhI CCWWGG 2 cut(s) 355, 668
Esp3I CGTCTC 2 cut(s) 396, 2532
FalI AAGNNNNNCTT 2 cut(s) 2616, 2648
FaqI GGGAC 3 cut(s) 112, 584, 1331
FauI CCCGC 3 cut(s) 806, 1589, 1708
Fnu4HI GCNGC 9 cut(s) 677, 1051, 1133, 1178, 2199, 2317, 2484, 3021, 3024
FokI GGATG 4 cut(s) 95, 2054, 2723, 2894
FriOI GRGCYC 2 cut(s) 1602, 2282
Fsp4HI GCNGC 9 cut(s) 677, 1051, 1133, 1178, 2199, 2317, 2484, 3021, 3024
FspBI CTAG 5 cut(s) 413, 422, 1326, 2375, 2982
GlaI GCGC 4 cut(s) 666, 798, 1975, 2893
GluI GCNGC 9 cut(s) 677, 1051, 1133, 1178, 2199, 2317, 2484, 3021, 3024
GsuI CTGGAG 4 cut(s) 1095, 2661, 2765, 2976
HaeII RGCGCY 1 cut(s) 668
HapII CCGG 9 cut(s) 258, 273, 374, 504, 519, 530, 1170, 1668, 1869
HgaI GACGC 3 cut(s) 522, 2060, 2801
HhaI GCGC 4 cut(s) 667, 799, 1976, 2894
Hin1I GRCGYC 4 cut(s) 533, 665, 2812, 2859
Hin6I GCGC 4 cut(s) 665, 797, 1974, 2892
HinP1I GCGC 4 cut(s) 665, 797, 1974, 2892
HincII GTYRAC 2 cut(s) 931, 993
HindII GTYRAC 2 cut(s) 931, 993
HindIII AAGCTT 3 cut(s) 482, 1205, 1852
HpaII CCGG 9 cut(s) 258, 273, 374, 504, 519, 530, 1170, 1668, 1869
Hpy166II GTNNAC 7 cut(s) 295, 931, 993, 1383, 2168, 2919, 3100
Hpy8I GTNNAC 7 cut(s) 295, 931, 993, 1383, 2168, 2919, 3100
Hpy99I CGWCG 1 cut(s) 473
HpyCH4III ACNGT 5 cut(s) 33, 233, 292, 2516, 2843
HpyCH4IV ACGT 3 cut(s) 1305, 2541, 2859
HpySE526I ACGT 3 cut(s) 1305, 2541, 2859
Hsp92I GRCGYC 4 cut(s) 533, 665, 2812, 2859
HspAI GCGC 4 cut(s) 665, 797, 1974, 2892
KasI GGCGCC 1 cut(s) 664
Kpn2I TCCGGA 1 cut(s) 1667
LmnI GCTCC 6 cut(s) 410, 553, 1246, 2480, 2680, 3031
Lsp1109I GCAGC 7 cut(s) 663, 1119, 1164, 2185, 2303, 2470, 3010
LweI GCATC 7 cut(s) 474, 933, 1492, 1588, 2515, 2542, 2919
MaeI CTAG 5 cut(s) 413, 422, 1326, 2375, 2982
MaeII ACGT 3 cut(s) 1305, 2541, 2859
MbiI CCGCTC 1 cut(s) 813
MflI RGATCY 3 cut(s) 489, 1815, 2967
MhlI GDGCHC 3 cut(s) 1602, 2282, 2921
MlsI TGGCCA 2 cut(s) 1764, 2606
MluNI TGGCCA 2 cut(s) 1764, 2606
Mly113I GGCGCC 1 cut(s) 665
MlyI GAGTC 4 cut(s) 212, 1561, 2561, 2898
MmeI TCCRAC 1 cut(s) 2439
Mox20I TGGCCA 2 cut(s) 1764, 2606
Mph1103I ATGCAT 2 cut(s) 1345, 2508
MroI TCCGGA 1 cut(s) 1667
MroXI GAANNNNTTC 1 cut(s) 1126
MscI TGGCCA 2 cut(s) 1764, 2606
MseI TTAA 8 cut(s) 35, 159, 164, 486, 684, 1544, 1964, 2006
MslI CAYNNNNRTG 1 cut(s) 2514
Msp20I TGGCCA 2 cut(s) 1764, 2606
MspA1I CMGCKG 2 cut(s) 2648, 3023
MspCI CTTAAG 2 cut(s) 485, 1963
MspI CCGG 9 cut(s) 258, 273, 374, 504, 519, 530, 1170, 1668, 1869
MspR9I CCNGG 4 cut(s) 374, 375, 1148, 2855
Mva1269I GAATGC 2 cut(s) 1126, 1345
MvaI CCWGG 2 cut(s) 1148, 2855
MvnI CGCG 2 cut(s) 726, 1976
NarI GGCGCC 1 cut(s) 665
NciI CCSGG 2 cut(s) 374, 375
NcoI CCATGG 1 cut(s) 668
NlaIV GGNNCC 8 cut(s) 6, 226, 666, 1600, 1601, 2659, 2676, 2766
NmeAIII GCCGAG 1 cut(s) 2886
NmuCI GTSAC 5 cut(s) 268, 326, 521, 614, 1629
NsiI ATGCAT 2 cut(s) 1345, 2508
NspI RCATGY 1 cut(s) 2294
NspV TTCGAA 2 cut(s) 2336, 2977
PaeR7I CTCGAG 1 cut(s) 3043
PagI TCATGA 1 cut(s) 2509
PceI AGGCCT 1 cut(s) 2944
PctI GAATGC 2 cut(s) 1126, 1345
PdmI GAANNNNTTC 1 cut(s) 1126
PflMI CCANNNNNTGG 1 cut(s) 1118
PinAI ACCGGT 1 cut(s) 1868
PkrI GCNGC 9 cut(s) 678, 1052, 1134, 1179, 2200, 2318, 2485, 3022, 3025
PleI GAGTC 4 cut(s) 212, 1561, 2561, 2898
PluTI GGCGCC 1 cut(s) 668
PpsI GAGTC 4 cut(s) 212, 1561, 2561, 2898
PshBI ATTAAT 1 cut(s) 2006
Psp124BI GAGCTC 1 cut(s) 2282
Psp6I CCWGG 2 cut(s) 1146, 2853
PspEI GGTNACC 1 cut(s) 614
PspGI CCWGG 2 cut(s) 1146, 2853
PspN4I GGNNCC 8 cut(s) 6, 226, 666, 1600, 1601, 2659, 2676, 2766
PspOMI GGGCCC 1 cut(s) 1598
PspPI GGNCC 8 cut(s) 956, 1598, 1599, 1670, 1717, 2214, 2491, 2851
PspXI VCTCGAGB 1 cut(s) 3043
PstNI CAGNNNCTG 2 cut(s) 221, 556
PsuI RGATCY 3 cut(s) 489, 1815, 2967
PvuII CAGCTG 1 cut(s) 2648
RsaI GTAC 4 cut(s) 451, 2169, 2182, 2357
RsaNI GTAC 4 cut(s) 450, 2168, 2181, 2356
RseI CAYNNNNRTG 1 cut(s) 2514
SacI GAGCTC 1 cut(s) 2282
SaqAI TTAA 8 cut(s) 35, 159, 164, 486, 684, 1544, 1964, 2006
SatI GCNGC 9 cut(s) 677, 1051, 1133, 1178, 2199, 2317, 2484, 3021, 3024
Sau96I GGNCC 8 cut(s) 956, 1598, 1599, 1670, 1717, 2214, 2491, 2851
ScaI AGTACT 1 cut(s) 2357
SchI GAGTC 4 cut(s) 212, 1561, 2561, 2898
ScrFI CCNGG 4 cut(s) 374, 375, 1148, 2855
SduI GDGCHC 3 cut(s) 1602, 2282, 2921
SfaNI GCATC 7 cut(s) 474, 933, 1492, 1588, 2515, 2542, 2919
SfoI GGCGCC 1 cut(s) 666
Sfr274I CTCGAG 1 cut(s) 3043
SfuI TTCGAA 2 cut(s) 2336, 2977
SinI GGWCC 4 cut(s) 1670, 1717, 2214, 2851
SlaI CTCGAG 1 cut(s) 3043
SmaI CCCGGG 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 2514
SpeI ACTAGT 1 cut(s) 2981
SseBI AGGCCT 1 cut(s) 2944
SsiI CCGC 8 cut(s) 88, 813, 828, 1051, 1596, 1701, 1976, 3021
SspDI GGCGCC 1 cut(s) 664
SspMI CTAG 5 cut(s) 413, 422, 1326, 2375, 2982
SstI GAGCTC 1 cut(s) 2282
StuI AGGCCT 1 cut(s) 2944
StyD4I CCNGG 4 cut(s) 372, 373, 1146, 2853
StyI CCWWGG 2 cut(s) 355, 668
TaaI ACNGT 5 cut(s) 33, 233, 292, 2516, 2843
TaiI ACGT 3 cut(s) 1308, 2544, 2862
TaqII GACCGA 1 cut(s) 1935
TatI WGTACW 1 cut(s) 2355
TauI GCSGC 2 cut(s) 1053, 3023
Tru1I TTAA 8 cut(s) 35, 159, 164, 486, 684, 1544, 1964, 2006
Tru9I TTAA 8 cut(s) 35, 159, 164, 486, 684, 1544, 1964, 2006
TscAI CASTG 6 cut(s) 236, 1246, 1894, 2332, 2481, 2848
TseFI GTSAC 5 cut(s) 268, 326, 521, 614, 1629
TseI GCWGC 7 cut(s) 676, 1132, 1177, 2198, 2316, 2483, 3023
Tsp45I GTSAC 5 cut(s) 268, 326, 521, 614, 1629
TspGWI ACGGA 3 cut(s) 256, 486, 1596
TspMI CCCGGG 1 cut(s) 373
TspRI CASTG 6 cut(s) 236, 1246, 1894, 2332, 2481, 2848
Van91I CCANNNNNTGG 1 cut(s) 1118
Vha464I CTTAAG 2 cut(s) 485, 1963
VneI GTGCAC 1 cut(s) 2917
VpaK11BI GGWCC 4 cut(s) 1670, 1717, 2214, 2851
VspI ATTAAT 1 cut(s) 2006
XapI RAATTY 4 cut(s) 733, 1348, 1746, 2086
XbaI TCTAGA 1 cut(s) 421
XceI RCATGY 1 cut(s) 2294
XhoI CTCGAG 1 cut(s) 3043
XmaI CCCGGG 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 1126
XspI CTAG 5 cut(s) 413, 422, 1326, 2375, 2982
ZraI GACGTC 1 cut(s) 2860
ZrmI AGTACT 1 cut(s) 2357
Zsp2I ATGCAT 2 cut(s) 1345, 2508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.