MD15G1196900.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001321
Physical Location & Seq
Forward (+)
15621520 .. 15624902
3383 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1196900.v1.1.491

Sequence Viewer

Length: 3069 bp
ATGACTAAACAAAACACCCCAACGTCTTCTCTCCAAACCCACCTCCACTTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCCCCCCCATGCCAACTCCCAATCCCTCCAAGACCAAGAACAAGCAGTCCTCGTGAAACTCAAGTCCTACTTAAACTCTCCGCCATTTCTCAGCCATTGGATCCTATCAAAATCAAACACTTCTTCCCACTGCTCCTGGCCTGAAATTTCCTGCACCAACAACTCCGTCACCAAATTGTTTCTTGACAACAAGAACATAACCCTACCGGTGCCATCCTTCATCTGTGATCTCAAAAACCTCACAGTCATTGATCTCAGCTACAACTACCTCCCAGGAGCATTCCCAAAAGCTGTCTACAAGTGTTCCAAGCTAGAGTACTTGGACCTGTCTCAGAACTACTTTGTAGGCCCCATTCCTGATGACATCGACAGCCTGCCCCGGCTTCAACAACTTATCCTCGCCGCAAACAACTTCTCCGGTGATATTCCTCCCGCCATAGGGCGGTTGCAAGAGCTCGAGAATCTTCAGCTGTACATGAACCACTTCAATGGCTCTGTCCCACCAGAAATAGGTAACTTGTCAAATCTCAAAGACCTGAGCATGTCTTTCAATATAAAACTTGTGCCTTGGAACTTGCCTTCCAATTTTACCAAGTTGAAAAAGCTCAAGACTTTATGGATACGGCAATCGAATTTGATCGGAGAGCTTCATGGGACACTTGGGGAGATGGAGGCACTTGAACAACTGGATTTGGCAATCAACAATTTGAGTGGGGAGATTCCGAGCGGTTTGTTTTCGTTGAAGAATTTAAGTACAATCTATCTCTTCATGAACAGGCTTTCTGGGGAGGTTCCTCGAGTGATCGAATCATTGAATTTGAGCATTCTTGATCTCTCTAACAACAATTTAACAGGGACAATACCAGAAGAGTATGGAAATCTTACCAAATTAACAGAATTGGCTTTGTTTTTCAATGGTTTTTCGGGTGAAGTTCCGAAGAGCATTGCCCGCCTGCCAAACCTCGTACATTTCAAAATCTTCAACAATAATTTGTCAGGAATATTGCCTCCAGAGTTTGGGAGGCACTCAAAACTCGAAGCTTTTGAAGTTTGTGTCAATAGGCTAACCGGAAAACTGCCAGACAATTTGTGCTATTGGGGTAAGCTGGAAACACTTATAGCTTATGATAATCATCTTAGTGGAGAATTGCCAAGTTCTCTTGGAAATTGCAGCAGTTTGAAGACTGTCAAGGTTCATGATAATTTACTGTCTGGAAACATTCCTAGTGGCATGTGGACGGCACCAAACTTGACTTCTATGCTGATAAGCAACAACTCGCTTACTGGCGAGCTTCCTGAGAAGCTTTCATCAAATCTTTCGCGGTTGGAAATGAGAGACAACAGATTTTCGGGCAACATTCCAATCGGGGTGTCTTCCTGGAGGAGTTTGATGGTTTTTGACGGCGGTAATAACCGCTTTAGTGGTGCTATTCCTCAGGAATTAACCGCGCTTTCTAGCTTATTGACACTTTCTCTTGATCAGAATCAGCTCACCGGCTTCCTTCCATCGGATATTGTATCGTGGGAGTCACTCACTAGTCTTAATTTCAGTCAAAATCAGCTCACCGGAACAATTCCTGAGAAACTTGGTCTTCTGCCGGGACTTACTGAATTAGACCTTTCAGCAAACCAACTTTCTGGCGAAATTCCAGTTCAACTCGGGCATCTGAAGCTCAACCAATTCAATCTCTCTTCCAATCACCTCTACGGGAAGATCCCTACTGAGTTTGAAAATGCTGCGTATGAAGGAAGCTTCTTGGACAATCGAGGCCTCTGCACAACTAGCTCTTCAGCAAAGCTCCCCATGTGCAATTCTGAATCCCAAAAGTCCAGCAAACTTTTGTCGAAATCTCTTGTGCTAATCTTATCTTTTGGCATACTGTTGTGCTTGTTGGCTATTTCCATCTCGTTCTTCATGGTCAGAAGTTACTGGAAGAGAAACGGTGGATTGGATTCTAAGTGGAAGCTCAACTCATTTCAGAGGTTGAATTTCACAGCGTCAAAAATTCTATCGGAGCTCACAGAAAGTAATCTGATTGGAAGTGGCGGGTCAGGAAAAGTTTATCGCGTTCCTGTGAATCGCAATGGTGATGTTGTTGCTGTGAAAAAGATTTGGAAGAATGAGAATTTAGAAAAGAAGCTTGAGAAAGAGTTTCTTGCAGAAGTCAATATCTTGAGCTCAATTCGACATGCCAACATAGTGAAGTTGATGTGCTGCATTTCCAGTGAGTCTTCAAAACTTCTCGTCTACGAGTACTCAGAAAATCGGAGCCTGGATCTATGGCTGCACAGGAGAAATAGGCCATCCAATCTCTCGAGGTCAGTCCATCATGTCGCACTCGACTGGCCTGAGAGGTTGCAGATTGCAGTGGGCGCTGCTCAGGGCCTCAGCTATATGCACCACGACTGTGTGCCGCCCGTGGTGCATCGCGACGTGAAATCAAGCAACATTTTGTTGGACTCTGATTTCAAAGCAAAAATAGCAGACTTTGGTCTGGCCAAGATGTTGGTCAAGCAAGGAGAACTTGCTATAATGTCATCTGTCGCTGGCTCTTTTGGCTACATTGCTCCAGAATATGCTCACACAATGCGATTGAATGAAAAGATCGACGTGTATAGCTTCGGGGTCATCCTTCTGGAGTTGACAACCGGCAGGGAAGCCAATGACGGCGATGAAGACACTGCCCTCGCTGAATGGGCGCGGTGTCACGTTCAAGAAAACAAAGCTATCGCTGATGCTTTGGACAAGGACATCAAAGAACCTTGTCACTTGGACGAAATGTGCTCCGTTTTCAAACTCGGCCTCATTTGCACTGAGAGACTTCCAGATGATAGGCCTTCCATGAAGGAGGTTCTGCAAATCTTGCTCCTATGCAGCCGTCCAGTACTCAGCAGAGAAAAGACCGAGCATGTTGCCTCTCCTCTGCTCCAAAACTCGAAGCACGAGAGGACCTTGGAAGACGATGATGGTAGTTTGGCGACCAATGTCTGA

Protein Analysis

1023

Amino Acids

113.58

Weight (kDa)

6.24

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 81 - 139 7.8e-08 Leucine rich repeat
LRR_14 PF23598 172 - 285 7.3e-07 Leucine-rich repeat region
LRR_14 PF23598 297 - 469 1.3e-06 Leucine-rich repeat region
LRR_8 PF13855 515 - 571 2.1e-06 Leucine rich repeat
Pkinase PF00069 701 - 979 2.9e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 702 - 980 2.5e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 286, 1318
AccB7I CCANNNNNTGG 1 cut(s) 1094
AccBSI CCGCTC 1 cut(s) 804
AccI GTMKAC 2 cut(s) 372, 2325
AccII CGCG 5 cut(s) 1399, 1526, 2145, 2508, 2779
AclWI GGATC 4 cut(s) 172, 185, 1786, 2361
AcoI YGGCCR 1 cut(s) 2574
AcsI RAATTY 8 cut(s) 222, 709, 823, 892, 1722, 2065, 2082, 2203
AcuI CTGAAG 3 cut(s) 527, 1766, 1851
AfaI GTAC 6 cut(s) 395, 551, 832, 1044, 2333, 2964
AfiI CCNNNNNNNGG 6 cut(s) 515, 516, 519, 587, 1094, 1585
AflIII ACRYGT 1 cut(s) 2688
AgeI ACCGGT 1 cut(s) 283
AhlI ACTAGT 1 cut(s) 1613
AjiI CACGTC 2 cut(s) 2512, 2689
AjnI CCWGG 4 cut(s) 212, 349, 1454, 2349
AjuI GAANNNNNNNTTGG 2 cut(s) 1315, 1347
AleI CACNNNNGTG 1 cut(s) 2484
AloI GAACNNNNNNTCC 4 cut(s) 108, 140, 641, 673
Alw21I GWGCWC 4 cut(s) 534, 2097, 2258, 2864
Alw26I GTCTC 3 cut(s) 411, 1407, 2890
AlwI GGATC 4 cut(s) 172, 185, 1786, 2361
Ama87I CYCGRG 4 cut(s) 533, 873, 1736, 2392
AoxI GGCC 9 cut(s) 215, 424, 1846, 2378, 2423, 2461, 2574, 2878, 2912
ApeKI GCWGC 6 cut(s) 1248, 1814, 2292, 2362, 2453, 2952
ApoI RAATTY 8 cut(s) 222, 709, 823, 892, 1722, 2065, 2082, 2203
AsiGI ACCGGT 1 cut(s) 283
Asp700I GAANNNNTTC 1 cut(s) 560
AspLEI GCGC 3 cut(s) 1528, 2453, 2779
AspS9I GGNCC 4 cut(s) 400, 425, 2461, 3027
AsuC2I CCSGG 2 cut(s) 457, 1677
AsuHPI GGTGA 7 cut(s) 238, 509, 1016, 1561, 1633, 1769, 2177
AvaI CYCGRG 4 cut(s) 533, 873, 1736, 2392
AvaII GGWCC 2 cut(s) 400, 3027
AxyI CCTNAGG 1 cut(s) 1512
BalI TGGCCA 1 cut(s) 2576
BamHI GGATCC 1 cut(s) 177
BanI GGYRCC 2 cut(s) 286, 1318
BanII GRGCYC 3 cut(s) 534, 2097, 2258
BauI CACGAG 2 cut(s) 128, 3020
BbsI GAAGAC 7 cut(s) 18, 1265, 1443, 1661, 2301, 2760, 3042
Bbv12I GWGCWC 4 cut(s) 534, 2097, 2258, 2864
BbvCI CCTCAGC 1 cut(s) 2465
BbvI GCAGC 6 cut(s) 1260, 1801, 2279, 2349, 2440, 2964
BccI CCATC 8 cut(s) 298, 739, 1462, 1591, 1988, 2389, 2412, 3038
BceAI ACGGC 5 cut(s) 716, 1332, 1495, 2761, 2940
BcgI CGANNNNNNTGC 4 cut(s) 1833, 1867, 2972, 3006
BciT130I CCWGG 4 cut(s) 214, 351, 1456, 2351
BciVI GTATCC 1 cut(s) 690
BclI TGATCA 1 cut(s) 1555
BcnI CCSGG 2 cut(s) 457, 1677
BcoDI GTCTC 3 cut(s) 411, 1407, 2890
BcuI ACTAGT 1 cut(s) 1613
BfaI CTAG 5 cut(s) 389, 1302, 1533, 1614, 1860
BfoI RGCGCY 1 cut(s) 2454
BfuI GTATCC 1 cut(s) 690
BisI GCNGC 8 cut(s) 480, 1249, 1815, 2293, 2363, 2454, 2492, 2953
BlsI GCNGC 8 cut(s) 481, 1250, 1816, 2294, 2364, 2455, 2493, 2954
BmcAI AGTACT 3 cut(s) 395, 2333, 2964
Bme1390I CCNGG 6 cut(s) 214, 351, 457, 1456, 1677, 2351
Bme18I GGWCC 2 cut(s) 400, 3027
BmeT110I CYCGRG 4 cut(s) 533, 873, 1736, 2392
BmgBI CACGTC 2 cut(s) 2512, 2689
BmgT120I GGNCC 4 cut(s) 400, 425, 2461, 3027
BmiI GGNNCC 6 cut(s) 179, 288, 427, 870, 1320, 2348
BmrFI CCNGG 6 cut(s) 214, 351, 457, 1456, 1677, 2351
BmsI GCATC 3 cut(s) 1750, 2512, 2803
BoxI GACNNNNGTC 2 cut(s) 2568, 3062
BpiI GAAGAC 7 cut(s) 18, 1265, 1443, 1661, 2301, 2760, 3042
BpmI CTGGAG 4 cut(s) 1071, 1477, 2631, 2735
Bpu10I CCTNAGC 3 cut(s) 614, 2457, 2465
BpuEI CTTGAG 4 cut(s) 122, 668, 2240, 2272
BpuMI CCSGG 2 cut(s) 457, 1677
BsaBI GATNNNNATC 2 cut(s) 1209, 1560
BsaJI CCNNGG 5 cut(s) 349, 455, 644, 2496, 3030
BsaWI WCCGGW 4 cut(s) 283, 494, 1145, 1643
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 6 cut(s) 515, 516, 519, 587, 1094, 1585
Bse118I RCCGGY 3 cut(s) 283, 1571, 2726
Bse1I ACTGG 7 cut(s) 768, 1366, 1727, 2012, 2301, 2426, 2960
Bse21I CCTNAGG 1 cut(s) 1512
Bse3DI GCAATG 3 cut(s) 1020, 2167, 2640
Bse8I GATNNNNATC 2 cut(s) 1209, 1560
BseBI CCWGG 4 cut(s) 214, 351, 1456, 2351
BseDI CCNNGG 5 cut(s) 349, 455, 644, 2496, 3030
BseGI GGATG 3 cut(s) 290, 2381, 2706
BseJI GATNNNNATC 2 cut(s) 1209, 1560
BseLI CCNNNNNNNGG 6 cut(s) 515, 516, 519, 587, 1094, 1585
BseMI GCAATG 3 cut(s) 1020, 2167, 2640
BseNI ACTGG 7 cut(s) 768, 1366, 1727, 2012, 2301, 2426, 2960
BseXI GCAGC 6 cut(s) 1260, 1801, 2279, 2349, 2440, 2964
BsgI GTGCAG 3 cut(s) 214, 1837, 2348
Bsh1236I CGCG 5 cut(s) 1399, 1526, 2145, 2508, 2779
BshFI GGCC 9 cut(s) 217, 426, 1848, 2380, 2425, 2463, 2576, 2880, 2914
BshNI GGYRCC 2 cut(s) 286, 1318
BshTI ACCGGT 1 cut(s) 283
BsiHKAI GWGCWC 4 cut(s) 534, 2097, 2258, 2864
BsiHKCI CYCGRG 4 cut(s) 533, 873, 1736, 2392
BsiSI CCGG 8 cut(s) 284, 457, 495, 1146, 1572, 1644, 1676, 2727
BslFI GGGAC 4 cut(s) 560, 745, 946, 1692
BslI CCNNNNNNNGG 6 cut(s) 515, 516, 519, 587, 1094, 1585
BsmAI GTCTC 3 cut(s) 411, 1407, 2890
BsmFI GGGAC 4 cut(s) 560, 745, 946, 1692
BsmI GAATGC 2 cut(s) 356, 900
BsnI GGCC 9 cut(s) 217, 426, 1848, 2380, 2425, 2463, 2576, 2880, 2914
BsoBI CYCGRG 4 cut(s) 533, 873, 1736, 2392
Bsp1286I GDGCHC 4 cut(s) 534, 2097, 2258, 2864
Bsp1407I TGTACA 1 cut(s) 549
Bsp68I TCGCGA 1 cut(s) 2508
BspANI GGCC 9 cut(s) 217, 426, 1848, 2380, 2425, 2463, 2576, 2880, 2914
BspFNI CGCG 5 cut(s) 1399, 1526, 2145, 2508, 2779
BspHI TCATGA 2 cut(s) 846, 1273
BspLI GGNNCC 6 cut(s) 179, 288, 427, 870, 1320, 2348
BspPI GGATC 4 cut(s) 172, 185, 1786, 2361
BspQI GCTCTTC 2 cut(s) 1010, 1870
BspT107I GGYRCC 2 cut(s) 286, 1318
BsrBI CCGCTC 1 cut(s) 804
BsrDI GCAATG 3 cut(s) 1020, 2167, 2640
BsrFI RCCGGY 3 cut(s) 283, 1571, 2726
BsrGI TGTACA 1 cut(s) 549
BsrI ACTGG 7 cut(s) 768, 1366, 1727, 2012, 2301, 2426, 2960
BssAI RCCGGY 3 cut(s) 283, 1571, 2726
BssECI CCNNGG 5 cut(s) 349, 455, 644, 2496, 3030
BssSI CACGAG 2 cut(s) 128, 3020
BssT1I CCWWGG 2 cut(s) 644, 3030
Bst2BI CACGAG 2 cut(s) 128, 3020
Bst2UI CCWGG 4 cut(s) 214, 351, 1456, 2351
Bst4CI ACNGT 6 cut(s) 322, 1264, 1287, 1959, 2021, 2486
Bst6I CTCTTC 6 cut(s) 848, 939, 1010, 1774, 1870, 2006
BstAPI GCANNNNNTGC 1 cut(s) 2941
BstAUI TGTACA 1 cut(s) 549
BstC8I GCNNGC 5 cut(s) 452, 1027, 1031, 1367, 2626
BstDSI CCRYGG 1 cut(s) 2496
BstF5I GGATG 3 cut(s) 290, 2381, 2706
BstFNI CGCG 5 cut(s) 1399, 1526, 2145, 2508, 2779
BstH2I RGCGCY 1 cut(s) 2454
BstHHI GCGC 3 cut(s) 1528, 2453, 2779
BstMAI GTCTC 3 cut(s) 411, 1407, 2890
BstNI CCWGG 4 cut(s) 214, 351, 1456, 2351
BstNSI RCATGY 4 cut(s) 622, 1312, 2270, 2990
BstPAI GACNNNNGTC 2 cut(s) 2568, 3062
BstSCI CCNGG 6 cut(s) 212, 349, 455, 1454, 1675, 2349
BstUI CGCG 5 cut(s) 1399, 1526, 2145, 2508, 2779
BstV1I GCAGC 6 cut(s) 1260, 1801, 2279, 2349, 2440, 2964
BstV2I GAAGAC 7 cut(s) 18, 1265, 1443, 1661, 2301, 2760, 3042
BstX2I RGATCY 3 cut(s) 177, 1791, 2353
BstXI CCANNNNNNTGG 3 cut(s) 566, 1715, 2584
BstYI RGATCY 3 cut(s) 177, 1791, 2353
Bsu36I CCTNAGG 1 cut(s) 1512
BsuI GTATCC 1 cut(s) 690
BsuRI GGCC 9 cut(s) 217, 426, 1848, 2380, 2425, 2463, 2576, 2880, 2914
BtgI CCRYGG 1 cut(s) 2496
BtgZI GCGATG 2 cut(s) 2489, 2763
BtrI CACGTC 2 cut(s) 2512, 2689
BtsCI GGATG 3 cut(s) 290, 2381, 2706
BtsI GCAGTG 3 cut(s) 205, 2451, 2757
BtsIMutI CAGTG 5 cut(s) 205, 2308, 2451, 2757, 2889
BtuMI TCGCGA 1 cut(s) 2508
Cac8I GCNNGC 5 cut(s) 452, 1027, 1031, 1367, 2626
CciI TCATGA 2 cut(s) 846, 1273
CfoI GCGC 3 cut(s) 1528, 2453, 2779
Cfr10I RCCGGY 3 cut(s) 283, 1571, 2726
Cfr13I GGNCC 4 cut(s) 400, 425, 2461, 3027
CseI GACGC 1 cut(s) 2064
Csp6I GTAC 6 cut(s) 394, 550, 831, 1043, 2332, 2963
CspAI ACCGGT 1 cut(s) 283
CspCI CAANNNNNGTGG 2 cut(s) 769, 804
CviQI GTAC 6 cut(s) 394, 550, 831, 1043, 2332, 2963
EaeI YGGCCR 1 cut(s) 2574
Eam1104I CTCTTC 6 cut(s) 848, 939, 1010, 1774, 1870, 2006
EarI CTCTTC 6 cut(s) 848, 939, 1010, 1774, 1870, 2006
EciI GGCGGA 1 cut(s) 147
Ecl136II GAGCTC 3 cut(s) 532, 2095, 2256
Eco130I CCWWGG 2 cut(s) 644, 3030
Eco147I AGGCCT 2 cut(s) 1848, 2914
Eco24I GRGCYC 3 cut(s) 534, 2097, 2258
Eco47I GGWCC 2 cut(s) 400, 3027
Eco53kI GAGCTC 3 cut(s) 532, 2095, 2256
Eco57I CTGAAG 3 cut(s) 527, 1766, 1851
Eco81I CCTNAGG 1 cut(s) 1512
Eco88I CYCGRG 4 cut(s) 533, 873, 1736, 2392
EcoICRI GAGCTC 3 cut(s) 532, 2095, 2256
EcoO109I RGGNCCY 3 cut(s) 425, 2461, 3027
EcoRII CCWGG 4 cut(s) 212, 349, 1454, 2349
EcoT14I CCWWGG 2 cut(s) 644, 3030
EcoT38I GRGCYC 3 cut(s) 534, 2097, 2258
ErhI CCWWGG 2 cut(s) 644, 3030
FalI AAGNNNNNCTT 6 cut(s) 131, 163, 2217, 2249, 2586, 2618
FaqI GGGAC 4 cut(s) 560, 745, 946, 1692
FauI CCCGC 3 cut(s) 517, 1034, 2117
FbaI TGATCA 1 cut(s) 1555
FblI GTMKAC 2 cut(s) 372, 2325
Fnu4HI GCNGC 8 cut(s) 480, 1249, 1815, 2293, 2363, 2454, 2492, 2953
FokI GGATG 3 cut(s) 277, 2368, 2693
FriOI GRGCYC 3 cut(s) 534, 2097, 2258
Fsp4HI GCNGC 8 cut(s) 480, 1249, 1815, 2293, 2363, 2454, 2492, 2953
FspBI CTAG 5 cut(s) 389, 1302, 1533, 1614, 1860
GlaI GCGC 3 cut(s) 1527, 2452, 2778
GluI GCNGC 8 cut(s) 480, 1249, 1815, 2293, 2363, 2454, 2492, 2953
GsuI CTGGAG 4 cut(s) 1071, 1477, 2631, 2735
HaeII RGCGCY 1 cut(s) 2454
HaeIII GGCC 9 cut(s) 217, 426, 1848, 2380, 2425, 2463, 2576, 2880, 2914
HapII CCGG 8 cut(s) 284, 457, 495, 1146, 1572, 1644, 1676, 2727
HgaI GACGC 1 cut(s) 2064
HhaI GCGC 3 cut(s) 1528, 2453, 2779
Hin6I GCGC 3 cut(s) 1526, 2451, 2777
HinP1I GCGC 3 cut(s) 1526, 2451, 2777
HincII GTYRAC 1 cut(s) 2721
HindII GTYRAC 1 cut(s) 2721
HindIII AAGCTT 4 cut(s) 1116, 1379, 1828, 2216
HpaII CCGG 8 cut(s) 284, 457, 495, 1146, 1572, 1644, 1676, 2727
HphI GGTGA 7 cut(s) 238, 509, 1016, 1561, 1633, 1769, 2177
Hpy166II GTNNAC 4 cut(s) 373, 1314, 2326, 2721
Hpy8I GTNNAC 4 cut(s) 373, 1314, 2326, 2721
Hpy99I CGWCG 2 cut(s) 2513, 2690
HpyAV CCTTC 7 cut(s) 304, 666, 1589, 1817, 2720, 2917, 2925
HpyCH4III ACNGT 6 cut(s) 322, 1264, 1287, 1959, 2021, 2486
HpyCH4IV ACGT 4 cut(s) 23, 2511, 2688, 2787
HpySE526I ACGT 4 cut(s) 23, 2511, 2688, 2787
HspAI GCGC 3 cut(s) 1526, 2451, 2777
Ksp22I TGATCA 1 cut(s) 1555
LguI GCTCTTC 2 cut(s) 1010, 1870
LmnI GCTCC 9 cut(s) 215, 353, 1881, 2092, 2346, 2650, 2867, 2949, 3009
Lsp1109I GCAGC 6 cut(s) 1260, 1801, 2279, 2349, 2440, 2964
LweI GCATC 3 cut(s) 1750, 2512, 2803
MaeI CTAG 5 cut(s) 389, 1302, 1533, 1614, 1860
MaeII ACGT 4 cut(s) 23, 2511, 2688, 2787
MaeIII GTNAC 6 cut(s) 244, 590, 1605, 2003, 2783, 2843
MbiI CCGCTC 1 cut(s) 804
MflI RGATCY 3 cut(s) 177, 1791, 2353
MhlI GDGCHC 4 cut(s) 534, 2097, 2258, 2864
MlsI TGGCCA 1 cut(s) 2576
MluNI TGGCCA 1 cut(s) 2576
MlyI GAGTC 3 cut(s) 1613, 2315, 2531
MmeI TCCRAC 2 cut(s) 1383, 2514
Mox20I TGGCCA 1 cut(s) 2576
MroXI GAANNNNTTC 1 cut(s) 560
MscI TGGCCA 1 cut(s) 2576
MseI TTAA 6 cut(s) 149, 827, 926, 968, 1520, 1620
MslI CAYNNNNRTG 3 cut(s) 564, 1215, 2484
Msp20I TGGCCA 1 cut(s) 2576
MspA1I CMGCKG 1 cut(s) 547
MspI CCGG 8 cut(s) 284, 457, 495, 1146, 1572, 1644, 1676, 2727
MspR9I CCNGG 6 cut(s) 214, 351, 457, 1456, 1677, 2351
Mva1269I GAATGC 2 cut(s) 356, 900
MvaI CCWGG 4 cut(s) 214, 351, 1456, 2351
MvnI CGCG 5 cut(s) 1399, 1526, 2145, 2508, 2779
NciI CCSGG 2 cut(s) 457, 1677
NlaIV GGNNCC 6 cut(s) 179, 288, 427, 870, 1320, 2348
NmeAIII GCCGAG 1 cut(s) 2856
NmuCI GTSAC 4 cut(s) 244, 1605, 2783, 2843
NruI TCGCGA 1 cut(s) 2508
NspI RCATGY 4 cut(s) 622, 1312, 2270, 2990
OliI CACNNNNGTG 1 cut(s) 2484
PaeR7I CTCGAG 3 cut(s) 533, 873, 2392
PagI TCATGA 2 cut(s) 846, 1273
PceI AGGCCT 2 cut(s) 1848, 2914
PciSI GCTCTTC 2 cut(s) 1010, 1870
PctI GAATGC 2 cut(s) 356, 900
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 7 cut(s) 538, 796, 884, 1561, 1895, 2030, 2155
PflMI CCANNNNNTGG 1 cut(s) 1094
PfoI TCCNGGA 1 cut(s) 1454
PinAI ACCGGT 1 cut(s) 283
PkrI GCNGC 8 cut(s) 481, 1250, 1816, 2294, 2364, 2455, 2493, 2954
PleI GAGTC 3 cut(s) 1612, 2314, 2531
PpsI GAGTC 3 cut(s) 1612, 2314, 2531
PpuMI RGGWCCY 1 cut(s) 3027
PshAI GACNNNNGTC 2 cut(s) 2568, 3062
Psp124BI GAGCTC 3 cut(s) 534, 2097, 2258
Psp5II RGGWCCY 1 cut(s) 3027
Psp6I CCWGG 4 cut(s) 212, 349, 1454, 2349
PspGI CCWGG 4 cut(s) 212, 349, 1454, 2349
PspN4I GGNNCC 6 cut(s) 179, 288, 427, 870, 1320, 2348
PspPI GGNCC 4 cut(s) 400, 425, 2461, 3027
PspPPI RGGWCCY 1 cut(s) 3027
PspXI VCTCGAGB 1 cut(s) 873
PsuI RGATCY 3 cut(s) 177, 1791, 2353
PvuII CAGCTG 1 cut(s) 547
RruI TCGCGA 1 cut(s) 2508
RsaI GTAC 6 cut(s) 395, 551, 832, 1044, 2333, 2964
RsaNI GTAC 6 cut(s) 394, 550, 831, 1043, 2332, 2963
RseI CAYNNNNRTG 3 cut(s) 564, 1215, 2484
SacI GAGCTC 3 cut(s) 534, 2097, 2258
SapI GCTCTTC 2 cut(s) 1010, 1870
SaqAI TTAA 6 cut(s) 149, 827, 926, 968, 1520, 1620
SatI GCNGC 8 cut(s) 480, 1249, 1815, 2293, 2363, 2454, 2492, 2953
Sau96I GGNCC 4 cut(s) 400, 425, 2461, 3027
ScaI AGTACT 3 cut(s) 395, 2333, 2964
SchI GAGTC 3 cut(s) 1613, 2315, 2531
ScrFI CCNGG 6 cut(s) 214, 351, 457, 1456, 1677, 2351
SduI GDGCHC 4 cut(s) 534, 2097, 2258, 2864
SfaNI GCATC 3 cut(s) 1750, 2512, 2803
Sfr274I CTCGAG 3 cut(s) 533, 873, 2392
SinI GGWCC 2 cut(s) 400, 3027
SlaI CTCGAG 3 cut(s) 533, 873, 2392
SmiMI CAYNNNNRTG 3 cut(s) 564, 1215, 2484
SmlI CTYRAG 7 cut(s) 137, 533, 683, 873, 2219, 2251, 2392
SmoI CTYRAG 7 cut(s) 137, 533, 683, 873, 2219, 2251, 2392
SpeI ACTAGT 1 cut(s) 1613
SseBI AGGCCT 2 cut(s) 1848, 2914
SspI AATATT 1 cut(s) 1080
SspMI CTAG 5 cut(s) 389, 1302, 1533, 1614, 1860
SstI GAGCTC 3 cut(s) 534, 2097, 2258
StuI AGGCCT 2 cut(s) 1848, 2914
StyD4I CCNGG 6 cut(s) 212, 349, 455, 1454, 1675, 2349
StyI CCWWGG 2 cut(s) 644, 3030
TaaI ACNGT 6 cut(s) 322, 1264, 1287, 1959, 2021, 2486
TaiI ACGT 4 cut(s) 26, 2514, 2691, 2790
TaqII GACCGA 1 cut(s) 2996
TatI WGTACW 5 cut(s) 393, 549, 830, 2331, 2962
TauI GCSGC 2 cut(s) 482, 2494
TfiI GAWTC 7 cut(s) 538, 796, 884, 1561, 1895, 2030, 2155
Tru1I TTAA 6 cut(s) 149, 827, 926, 968, 1520, 1620
Tru9I TTAA 6 cut(s) 149, 827, 926, 968, 1520, 1620
TscAI CASTG 5 cut(s) 212, 2308, 2451, 2764, 2896
TseFI GTSAC 4 cut(s) 244, 1605, 2783, 2843
TseI GCWGC 6 cut(s) 1248, 1814, 2292, 2362, 2453, 2952
Tsp45I GTSAC 4 cut(s) 244, 1605, 2783, 2843
TspGWI ACGGA 2 cut(s) 232, 2854
TspRI CASTG 5 cut(s) 212, 2308, 2451, 2764, 2896
Van91I CCANNNNNTGG 1 cut(s) 1094
VpaK11BI GGWCC 2 cut(s) 400, 3027
XapI RAATTY 8 cut(s) 222, 709, 823, 892, 1722, 2065, 2082, 2203
XceI RCATGY 4 cut(s) 622, 1312, 2270, 2990
XhoI CTCGAG 3 cut(s) 533, 873, 2392
XmiI GTMKAC 2 cut(s) 372, 2325
XmnI GAANNNNTTC 1 cut(s) 560
XspI CTAG 5 cut(s) 389, 1302, 1533, 1614, 1860
ZrmI AGTACT 3 cut(s) 395, 2333, 2964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.