FvH4_3g06480

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001647
Physical Location & Seq
Forward (+)
3721653 .. 3723129
1477 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06480.t1

Sequence Viewer

Length: 318 bp
ATGGTGAACGTTCCCAAGACAAAGAAGACCTACTGCAAGAGCAAGGAGTGCAAGAAGCACACCTTGCACAAGGTGACGCAGTACAAGAAGGGCAAGGACAGTCTTGCGGCTCAGGGAAAGCGTCGTTATGATCGCAAGCAATCTGGATATGGAGGTCAGACCAAGCCAGTCTTTCACAAGAAGGCCAAGACTACCAAGAAGATTGTTCTAAGGCTTCAATGCCAGGGATGCAAGCACGTTTCTCAGCATCCAATCAAGAGGTGCAAGCACTTCGAGATCGGTGGAGACAAGAAGGGAAAGGGAACATCGCTTTTTTAA

Protein Analysis

106

Amino Acids

12.0

Weight (kDa)

10.29

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L44 PF00935 19 - 94 1.4e-33 Ribosomal protein L44
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 107
AclI AACGTT 1 cut(s) 9
AdeI CACNNNGTG 1 cut(s) 73
AfaI GTAC 1 cut(s) 83
AgsI TTSAA 1 cut(s) 218
AjnI CCWGG 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 279
AoxI GGCC 1 cut(s) 183
AsuHPI GGTGA 2 cut(s) 16, 85
BbsI GAAGAC 1 cut(s) 32
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 279
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 224
BmrFI CCNGG 1 cut(s) 224
BmsI GCATC 2 cut(s) 218, 256
BpiI GAAGAC 1 cut(s) 32
Bpu10I CCTNAGC 1 cut(s) 111
BsaJI CCNNGG 1 cut(s) 223
Bse1I ACTGG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 2 cut(s) 233, 247
BseMII CTCAG 2 cut(s) 125, 257
BseNI ACTGG 1 cut(s) 167
BshFI GGCC 1 cut(s) 185
BsmAI GTCTC 1 cut(s) 279
BsnI GGCC 1 cut(s) 185
Bsp143I GATC 2 cut(s) 130, 276
BspACI CCGC 1 cut(s) 107
BspANI GGCC 1 cut(s) 185
BspCNI CTCAG 2 cut(s) 124, 256
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 2 cut(s) 130, 276
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 1 cut(s) 101
BstAPI GCANNNNNTGC 2 cut(s) 48, 64
BstC8I GCNNGC 3 cut(s) 137, 233, 266
BstDEI CTNAG 3 cut(s) 111, 209, 243
BstF5I GGATG 2 cut(s) 233, 247
BstKTI GATC 2 cut(s) 133, 279
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 2 cut(s) 130, 276
BstMWI GCNNNNNNNGC 3 cut(s) 48, 64, 228
BstNI CCWGG 1 cut(s) 224
BstSCI CCNGG 1 cut(s) 222
BstV2I GAAGAC 1 cut(s) 32
BsuRI GGCC 1 cut(s) 185
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 2 cut(s) 233, 247
Cac8I GCNNGC 3 cut(s) 137, 233, 266
CseI GACGC 2 cut(s) 85, 110
Csp6I GTAC 1 cut(s) 82
CviJI RGCY 4 cut(s) 110, 166, 185, 214
CviKI_1 RGCY 4 cut(s) 110, 166, 185, 214
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 3 cut(s) 111, 209, 243
DpnI GATC 2 cut(s) 132, 278
DpnII GATC 2 cut(s) 130, 276
DraIII CACNNNGTG 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 222
FaiI YATR 2 cut(s) 129, 150
FalI AAGNNNNNCTT 4 cut(s) 47, 79, 155, 187
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 2 cut(s) 234, 240
Fsp4HI GCNGC 1 cut(s) 108
GluI GCNGC 1 cut(s) 108
HaeIII GGCC 1 cut(s) 185
HgaI GACGC 2 cut(s) 85, 110
HphI GGTGA 2 cut(s) 16, 85
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 159
Hpy188III TCNNGA 3 cut(s) 144, 256, 274
Hpy8I GTNNAC 1 cut(s) 7
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 3 cut(s) 82, 175, 286
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 2 cut(s) 9, 237
HpyCH4V TGCA 5 cut(s) 36, 51, 67, 231, 264
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 64, 228
HpyF3I CTNAG 3 cut(s) 111, 209, 243
HpySE526I ACGT 2 cut(s) 9, 237
Kzo9I GATC 2 cut(s) 130, 276
LpnPI CCDG 5 cut(s) 98, 129, 180, 209, 236
LweI GCATC 2 cut(s) 218, 256
MaeII ACGT 2 cut(s) 9, 237
MaeIII GTNAC 1 cut(s) 73
MalI GATC 2 cut(s) 132, 278
MboI GATC 2 cut(s) 130, 276
MboII GAAGA 2 cut(s) 37, 211
MnlI CCTC 2 cut(s) 146, 252
MseI TTAA 1 cut(s) 316
MspR9I CCNGG 1 cut(s) 224
MvaI CCWGG 1 cut(s) 224
MwoI GCNNNNNNNGC 3 cut(s) 48, 64, 228
NdeII GATC 2 cut(s) 130, 276
NmuCI GTSAC 1 cut(s) 73
PkrI GCNGC 1 cut(s) 109
Psp1406I AACGTT 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 222
PspGI CCWGG 1 cut(s) 222
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SaqAI TTAA 1 cut(s) 316
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 2 cut(s) 130, 276
ScrFI CCNGG 1 cut(s) 224
SetI ASST 7 cut(s) 12, 32, 65, 75, 157, 240, 263
SfaNI GCATC 2 cut(s) 218, 256
SsiI CCGC 1 cut(s) 107
StyD4I CCNGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 2 cut(s) 12, 240
TaqI TCGA 1 cut(s) 273
TatI WGTACW 1 cut(s) 81
TauI GCSGC 1 cut(s) 110
Tru1I TTAA 1 cut(s) 316
Tru9I TTAA 1 cut(s) 316
TseFI GTSAC 1 cut(s) 73
Tsp45I GTSAC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.