MD10G1269000.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001647
Physical Location & Seq
Reverse (-)
36168669 .. 36170167
1499 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1269000.v1.1.491

Sequence Viewer

Length: 318 bp
ATGGTGAACGTTCCCAAGACCAAGAAGACCTACTGCAAGAGCAAGGAGTGCAAGAAGCACACCTTGCACAAGGTTACACAATACAAGAAGGGCAAGGATAGTCTTGCTGCTCAGGGAAAGCGTCGTTACGATCGCAAGCAGTCTGGTTATGGTGGACAGACAAAGCCAGTCTTCCACAAGAAGGCAAAGACTACAAAGAAGATTGTGTTGAGGCTCCAGTGCCAGGGATGCAAACATGTATCCCAGCACCCGATCAAGAGGTGCAAGCACTTTGAAATCGGTGGAGACAAGAAGGGAAAGGGAACATCTCTGTTTTAA

Protein Analysis

106

Amino Acids

12.0

Weight (kDa)

10.29

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L44 PF00935 19 - 94 1.4e-33 Ribosomal protein L44
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 9
AfiI CCNNNNNNNGG 2 cut(s) 181, 223
AflIII ACRYGT 1 cut(s) 235
AgsI TTSAA 1 cut(s) 275
AjnI CCWGG 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 279
ApeKI GCWGC 1 cut(s) 107
AsuHPI GGTGA 1 cut(s) 16
BbsI GAAGAC 2 cut(s) 32, 163
BbvI GCAGC 1 cut(s) 94
BciT130I CCWGG 1 cut(s) 224
BciVI GTATCC 1 cut(s) 250
BcoDI GTCTC 1 cut(s) 279
BfuI GTATCC 1 cut(s) 250
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 224
BmiI GGNNCC 1 cut(s) 215
BmrFI CCNGG 1 cut(s) 224
BmsI GCATC 1 cut(s) 218
BpiI GAAGAC 2 cut(s) 32, 163
BpmI CTGGAG 1 cut(s) 200
Bpu10I CCTNAGC 1 cut(s) 111
BsaJI CCNNGG 1 cut(s) 223
Bsc4I CCNNNNNNNGG 2 cut(s) 181, 223
Bse1I ACTGG 2 cut(s) 167, 217
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 1 cut(s) 233
BseLI CCNNNNNNNGG 2 cut(s) 181, 223
BseMII CTCAG 1 cut(s) 125
BseNI ACTGG 2 cut(s) 167, 217
BseXI GCAGC 1 cut(s) 94
BseYI CCCAGC 1 cut(s) 243
Bsh1285I CGRYCG 1 cut(s) 133
BsiEI CGRYCG 1 cut(s) 133
BslI CCNNNNNNNGG 2 cut(s) 181, 223
BsmAI GTCTC 1 cut(s) 279
Bsp143I GATC 2 cut(s) 130, 252
BspCNI CTCAG 1 cut(s) 124
BspLI GGNNCC 1 cut(s) 215
BsrI ACTGG 2 cut(s) 167, 217
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 2 cut(s) 130, 252
Bst2UI CCWGG 1 cut(s) 224
BstAPI GCANNNNNTGC 2 cut(s) 48, 64
BstC8I GCNNGC 2 cut(s) 137, 266
BstDEI CTNAG 1 cut(s) 111
BstF5I GGATG 1 cut(s) 233
BstKTI GATC 2 cut(s) 133, 255
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 2 cut(s) 130, 252
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 3 cut(s) 48, 64, 228
BstNI CCWGG 1 cut(s) 224
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 2 cut(s) 32, 163
BsuI GTATCC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 233
BtsIMutI CAGTG 1 cut(s) 224
Cac8I GCNNGC 2 cut(s) 137, 266
CseI GACGC 1 cut(s) 110
CviAII CATG 1 cut(s) 236
CviJI RGCY 2 cut(s) 166, 214
CviKI_1 RGCY 2 cut(s) 166, 214
DdeI CTNAG 1 cut(s) 111
DpnI GATC 2 cut(s) 132, 254
DpnII GATC 2 cut(s) 130, 252
EcoRII CCWGG 1 cut(s) 222
FaeI CATG 1 cut(s) 239
FaiI YATR 2 cut(s) 150, 237
FalI AAGNNNNNCTT 4 cut(s) 47, 79, 155, 187
FatI CATG 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 108
GluI GCNGC 1 cut(s) 108
GsaI CCCAGC 1 cut(s) 247
GsuI CTGGAG 1 cut(s) 200
HgaI GACGC 1 cut(s) 110
Hin1II CATG 1 cut(s) 239
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 7, 155
Hpy188III TCNNGA 1 cut(s) 256
Hpy8I GTNNAC 2 cut(s) 7, 155
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 3 cut(s) 82, 175, 286
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 5 cut(s) 36, 51, 67, 231, 264
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 64, 228
HpyF3I CTNAG 1 cut(s) 111
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 1 cut(s) 239
Kzo9I GATC 2 cut(s) 130, 252
LmnI GCTCC 1 cut(s) 219
LpnPI CCDG 7 cut(s) 98, 129, 180, 209, 230, 236, 257
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 1 cut(s) 218
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 2 cut(s) 73, 125
MalI GATC 2 cut(s) 132, 254
MboI GATC 2 cut(s) 130, 252
MboII GAAGA 3 cut(s) 37, 163, 211
MnlI CCTC 2 cut(s) 204, 252
MseI TTAA 1 cut(s) 316
MspR9I CCNGG 1 cut(s) 224
MvaI CCWGG 1 cut(s) 224
MwoI GCNNNNNNNGC 3 cut(s) 48, 64, 228
NdeII GATC 2 cut(s) 130, 252
NlaIII CATG 1 cut(s) 239
NlaIV GGNNCC 1 cut(s) 215
NspI RCATGY 1 cut(s) 239
PciI ACATGT 1 cut(s) 235
PkrI GCNGC 1 cut(s) 109
Ple19I CGATCG 1 cut(s) 133
PscI ACATGT 1 cut(s) 235
Psp1406I AACGTT 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 222
PspFI CCCAGC 1 cut(s) 243
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 215
PvuI CGATCG 1 cut(s) 133
SaqAI TTAA 1 cut(s) 316
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 2 cut(s) 130, 252
ScrFI CCNGG 1 cut(s) 224
SetI ASST 5 cut(s) 12, 32, 65, 75, 263
SfaNI GCATC 1 cut(s) 218
StyD4I CCNGG 1 cut(s) 222
TaiI ACGT 1 cut(s) 12
Tru1I TTAA 1 cut(s) 316
Tru9I TTAA 1 cut(s) 316
TscAI CASTG 1 cut(s) 224
TseI GCWGC 1 cut(s) 107
TspRI CASTG 1 cut(s) 224
XceI RCATGY 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.