Rorug05G0004400

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001647
Physical Location & Seq
Reverse (-)
296625 .. 298934
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0004400.1

Sequence Viewer

Length: 1281 bp
ATGGAGGTGTTGACCGGCGACCGGAATATACCAGACCCAGCAGCGGCAGCGGCAGCGGCAGCAGAAGAAGAACAAGTCTTCCCGGCGCACATATCCCCATTCTCTGAGACACACCAAGTTGTCTTCGCCAATCCAACGGCGGCTGCTATGACTATGACTATGACTATGACGCCGCAGAAGCTCCGTATCAGGTGCAACGGAAGGTCTCCTGCCGTCGACGCAGCACCAACTCAGCTAGATCCGCCCCAACTCGGCTCCGCTGACAACGGCCTGTCACCACATAATGCTGATGAGCATTTTAAAGCACAAGGGGTAGTAGAATTCGTGAATTGGAAGCAGAAGAGGAAGAGAACATCATCTTGTACTAGTACAAGAAAAAAGAGCAAAAGGCTTGAAATGCTCGAGCATTTTCTGGAAAGTCTGGTAAACAAAATGATGGAGAAGCAAGAGCAGATGCATAAGCAGTTGATAGAGATGGTACAAGACAGGGAGAAAGAGAGAATAGCCAAAGAAGAAGCCTGGAAACAGCACGAGTTGGAGAGAATCACAAGGGATGAGGAGGTAAGAGCCCAAGAGACATCGCGCAGCCTTGCCCTCATTAACTTTGTACAGAGTTTGTCGGGCAGTGATGTTCAAGTTCCCCAACCAGCACCACTCGTAGCACAACCAGTGGCAACAGTACAACCTCCTACTTCCAATCAATATCATCAACGTGTGGAAGAAAATAAAAATCTTCACAAAGACACAGCGATTGCCAAGTGTATTGCAACACCAACTGATAAAAGATGGCTCCAATTTGAAGTACGGGCACTCATAGCGCTGCGGACTAGTTTGGAACATAAGTTTCACACCCCAATTAATTCTAGCCATGGATCTCTATGGGAGGAGATAGCTCTTGGGATGAACCGTATGGGCTTTGATCGCTCAGGAAGGAAGTGTAAAGAAAAATGGGAAAACATGAACAAGTACTTCAAAAGGGCAATCACAAATGACAAGAAGCGTCCTGCAAATGCTAAGACTTGTCTGTATTTTAATGAATTGGAGTTGCTCTACAAAAACGGACTTCTAAATCTGGGCAATGGCTTTAGTCCCACAAGCAGTCTGGGGAATGGCTTTAGTTGCACAAGCAACCAGAAGCAGACCAAGAGTGAAAACGAGTCCCAACAGTGCGTGCTCAGCAACGTTGACACTAGTCTTCTAACTTGTGCCACAATACCATCGGTGTTAAATTCAAATGAAGCTGCCGAAAGAACGAGAGCAGTGGTTTCTCCTGCTCTTTAA

Protein Analysis

426

Amino Acids

47.79

Weight (kDa)

8.67

Isoelectric Point (pI)

54.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 261 - 349 4.1e-19 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 216
AccII CGCG 1 cut(s) 583
AciI CCGC 8 cut(s) 44, 50, 56, 140, 173, 242, 258, 823
AclI AACGTT 1 cut(s) 1182
AclWI GGATC 2 cut(s) 233, 880
AcsI RAATTY 2 cut(s) 320, 1228
AcyI GRCGYC 1 cut(s) 170
AfaI GTAC 7 cut(s) 364, 370, 480, 609, 681, 804, 968
AfeI AGCGCT 1 cut(s) 819
AfiI CCNNNNNNNGG 3 cut(s) 21, 43, 251
AflIII ACRYGT 1 cut(s) 712
AgsI TTSAA 5 cut(s) 395, 635, 800, 973, 1233
AhlI ACTAGT 3 cut(s) 365, 827, 1190
AjnI CCWGG 1 cut(s) 518
AloI GAACNNNNNNTCC 2 cut(s) 63, 95
AluBI AGCT 4 cut(s) 181, 235, 893, 1241
AluI AGCT 4 cut(s) 181, 235, 893, 1241
Alw21I GWGCWC 1 cut(s) 1176
Alw26I GTCTC 3 cut(s) 101, 210, 569
AlwI GGATC 2 cut(s) 233, 880
Ama87I CYCGRG 1 cut(s) 401
Aor51HI AGCGCT 1 cut(s) 819
AoxI GGCC 1 cut(s) 268
ApeKI GCWGC 9 cut(s) 41, 47, 53, 59, 143, 221, 585, 820, 1241
ApoI RAATTY 2 cut(s) 320, 1228
AseI ATTAAT 1 cut(s) 858
AspLEI GCGC 3 cut(s) 88, 585, 820
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 267
AvaI CYCGRG 1 cut(s) 401
BaeGI GKGCMC 1 cut(s) 811
BanII GRGCYC 1 cut(s) 571
BauI CACGAG 1 cut(s) 530
BbsI GAAGAC 3 cut(s) 70, 115, 1187
Bbv12I GWGCWC 1 cut(s) 1176
BbvI GCAGC 9 cut(s) 53, 59, 65, 71, 130, 233, 597, 807, 1228
BccI CCATC 4 cut(s) 430, 469, 780, 1225
BceAI ACGGC 3 cut(s) 153, 197, 283
BciT130I CCWGG 1 cut(s) 520
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 3 cut(s) 101, 210, 569
BcuI ACTAGT 3 cut(s) 365, 827, 1190
BfaI CTAG 5 cut(s) 236, 366, 828, 864, 1191
BfoI RGCGCY 1 cut(s) 821
BlpI GCTNAGC 1 cut(s) 1175
BmcAI AGTACT 1 cut(s) 968
Bme1390I CCNGG 2 cut(s) 83, 520
BmeT110I CYCGRG 1 cut(s) 401
BmiI GGNNCC 2 cut(s) 256, 791
BmrFI CCNGG 2 cut(s) 83, 520
BmsI GCATC 1 cut(s) 444
BoxI GACNNNNGTC 1 cut(s) 1191
BpiI GAAGAC 3 cut(s) 70, 115, 1187
Bpu10I CCTNAGC 1 cut(s) 925
Bpu1102I GCTNAGC 1 cut(s) 1175
BpuMI CCSGG 1 cut(s) 83
BsaHI GRCGYC 1 cut(s) 170
BsaI GGTCTC 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 868
BsaWI WCCGGW 1 cut(s) 21
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 43, 251
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 1 cut(s) 668
Bse3DI GCAATG 1 cut(s) 1084
BseBI CCWGG 1 cut(s) 520
BseDI CCNNGG 1 cut(s) 868
BseGI GGATG 2 cut(s) 559, 906
BseLI CCNNNNNNNGG 3 cut(s) 21, 43, 251
BseMI GCAATG 1 cut(s) 1084
BseMII CTCAG 4 cut(s) 96, 245, 939, 1189
BseNI ACTGG 1 cut(s) 668
BseRI GAGGAG 2 cut(s) 572, 899
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 9 cut(s) 53, 59, 65, 71, 130, 233, 597, 807, 1228
BseYI CCCAGC 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 583
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 1 cut(s) 270
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 1176
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 3 cut(s) 15, 22, 83
BslFI GGGAC 2 cut(s) 1074, 1144
BslI CCNNNNNNNGG 3 cut(s) 21, 43, 251
BsmAI GTCTC 3 cut(s) 101, 210, 569
BsmFI GGGAC 2 cut(s) 1074, 1144
BsnI GGCC 1 cut(s) 270
Bso31I GGTCTC 1 cut(s) 210
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 3 cut(s) 571, 811, 1176
Bsp1407I TGTACA 1 cut(s) 607
Bsp143I GATC 3 cut(s) 238, 872, 919
Bsp1720I GCTNAGC 1 cut(s) 1175
Bsp19I CCATGG 1 cut(s) 868
BspACI CCGC 8 cut(s) 44, 50, 56, 140, 173, 242, 258, 823
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 4 cut(s) 97, 244, 938, 1188
BspFNI CGCG 1 cut(s) 583
BspLI GGNNCC 2 cut(s) 256, 791
BspPI GGATC 2 cut(s) 233, 880
BspTNI GGTCTC 1 cut(s) 210
BsrDI GCAATG 1 cut(s) 1084
BsrFI RCCGGY 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 607
BsrI ACTGG 1 cut(s) 668
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 1 cut(s) 868
BssMI GATC 3 cut(s) 238, 872, 919
BssNI GRCGYC 1 cut(s) 170
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 868
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 1 cut(s) 520
Bst4CI ACNGT 3 cut(s) 679, 908, 1167
Bst6I CTCTTC 2 cut(s) 335, 341
BstACI GRCGYC 1 cut(s) 170
BstAUI TGTACA 1 cut(s) 607
BstC8I GCNNGC 1 cut(s) 1172
BstDEI CTNAG 5 cut(s) 105, 231, 925, 1014, 1175
BstDSI CCRYGG 1 cut(s) 868
BstF5I GGATG 2 cut(s) 559, 906
BstFNI CGCG 1 cut(s) 583
BstH2I RGCGCY 1 cut(s) 821
BstHHI GCGC 3 cut(s) 88, 585, 820
BstKTI GATC 3 cut(s) 241, 875, 922
BstMAI GTCTC 3 cut(s) 101, 210, 569
BstMBI GATC 3 cut(s) 238, 872, 919
BstMCI CGRYCG 1 cut(s) 22
BstNI CCWGG 1 cut(s) 520
BstPAI GACNNNNGTC 1 cut(s) 1191
BstSCI CCNGG 2 cut(s) 81, 518
BstSLI GKGCMC 1 cut(s) 811
BstUI CGCG 1 cut(s) 583
BstV1I GCAGC 9 cut(s) 53, 59, 65, 71, 130, 233, 597, 807, 1228
BstV2I GAAGAC 3 cut(s) 70, 115, 1187
BstX2I RGATCY 2 cut(s) 238, 872
BstYI RGATCY 2 cut(s) 238, 872
BsuRI GGCC 1 cut(s) 270
BtgI CCRYGG 1 cut(s) 868
BtgZI GCGATG 1 cut(s) 564
BtsCI GGATG 2 cut(s) 559, 906
BtsI GCAGTG 2 cut(s) 631, 1266
BtsIMutI CAGTG 4 cut(s) 631, 675, 1172, 1266
Cac8I GCNNGC 1 cut(s) 1172
CfoI GCGC 3 cut(s) 88, 585, 820
Cfr10I RCCGGY 1 cut(s) 14
CseI GACGC 3 cut(s) 178, 227, 989
Csp6I GTAC 7 cut(s) 363, 369, 479, 608, 680, 803, 967
CviAII CATG 2 cut(s) 869, 958
CviQI GTAC 7 cut(s) 363, 369, 479, 608, 680, 803, 967
DdeI CTNAG 5 cut(s) 105, 231, 925, 1014, 1175
DpnI GATC 3 cut(s) 240, 874, 921
DpnII GATC 3 cut(s) 238, 872, 919
DraI TTTAAA 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 335, 341
EarI CTCTTC 2 cut(s) 335, 341
EciI GGCGGA 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 868
Eco24I GRGCYC 1 cut(s) 571
Eco31I GGTCTC 1 cut(s) 210
Eco47III AGCGCT 1 cut(s) 819
Eco88I CYCGRG 1 cut(s) 401
EcoRI GAATTC 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 518
EcoT14I CCWWGG 1 cut(s) 868
EcoT22I ATGCAT 1 cut(s) 459
EcoT38I GRGCYC 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 868
FaeI CATG 2 cut(s) 872, 961
FaqI GGGAC 2 cut(s) 1074, 1144
FatI CATG 2 cut(s) 868, 957
FblI GTMKAC 1 cut(s) 216
FokI GGATG 2 cut(s) 566, 913
FriOI GRGCYC 1 cut(s) 571
FspBI CTAG 5 cut(s) 236, 366, 828, 864, 1191
GlaI GCGC 3 cut(s) 87, 584, 819
GsaI CCCAGC 1 cut(s) 41
HaeII RGCGCY 1 cut(s) 821
HaeIII GGCC 1 cut(s) 270
HapII CCGG 3 cut(s) 15, 22, 83
HgaI GACGC 3 cut(s) 178, 227, 989
HhaI GCGC 3 cut(s) 88, 585, 820
Hin1I GRCGYC 1 cut(s) 170
Hin1II CATG 2 cut(s) 872, 961
Hin6I GCGC 3 cut(s) 86, 583, 818
HinP1I GCGC 3 cut(s) 86, 583, 818
HincII GTYRAC 3 cut(s) 12, 217, 1186
HindII GTYRAC 3 cut(s) 12, 217, 1186
HinfI GANTC 2 cut(s) 543, 1157
HpaII CCGG 3 cut(s) 15, 22, 83
HphI GGTGA 1 cut(s) 267
Hpy166II GTNNAC 4 cut(s) 12, 217, 427, 1186
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 3 cut(s) 325, 413, 927
Hpy8I GTNNAC 4 cut(s) 12, 217, 427, 1186
Hpy99I CGWCG 2 cut(s) 218, 221
HpyAV CCTTC 2 cut(s) 195, 924
HpyCH4III ACNGT 3 cut(s) 679, 908, 1167
HpyCH4IV ACGT 2 cut(s) 712, 1182
HpyCH4V TGCA 5 cut(s) 195, 457, 767, 1007, 1122
HpyF3I CTNAG 5 cut(s) 105, 231, 925, 1014, 1175
HpySE526I ACGT 2 cut(s) 712, 1182
Hsp92I GRCGYC 1 cut(s) 170
Hsp92II CATG 2 cut(s) 872, 961
HspAI GCGC 3 cut(s) 86, 583, 818
Kzo9I GATC 3 cut(s) 238, 872, 919
LmnI GCTCC 3 cut(s) 186, 260, 795
Lsp1109I GCAGC 9 cut(s) 53, 59, 65, 71, 130, 233, 597, 807, 1228
LweI GCATC 1 cut(s) 444
MaeI CTAG 5 cut(s) 236, 366, 828, 864, 1191
MaeII ACGT 2 cut(s) 712, 1182
MaeIII GTNAC 1 cut(s) 273
MalI GATC 3 cut(s) 240, 874, 921
MboI GATC 3 cut(s) 238, 872, 919
MflI RGATCY 2 cut(s) 238, 872
MhlI GDGCHC 3 cut(s) 571, 811, 1176
MluCI AATT 7 cut(s) 320, 328, 794, 855, 859, 1037, 1228
MlyI GAGTC 1 cut(s) 1166
MmeI TCCRAC 2 cut(s) 158, 516
MnlI CCTC 6 cut(s) 336, 550, 553, 605, 696, 877
Mph1103I ATGCAT 1 cut(s) 459
MseI TTAA 6 cut(s) 300, 600, 858, 1032, 1226, 1279
MslI CAYNNNNRTG 1 cut(s) 711
MspA1I CMGCKG 4 cut(s) 44, 50, 56, 260
MspI CCGG 3 cut(s) 15, 22, 83
MspR9I CCNGG 2 cut(s) 83, 520
MvaI CCWGG 1 cut(s) 520
MvnI CGCG 1 cut(s) 583
NciI CCSGG 1 cut(s) 83
NcoI CCATGG 1 cut(s) 868
NdeII GATC 3 cut(s) 238, 872, 919
NlaIII CATG 2 cut(s) 872, 961
NlaIV GGNNCC 2 cut(s) 256, 791
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 273
NsiI ATGCAT 1 cut(s) 459
PaeR7I CTCGAG 1 cut(s) 401
PfeI GAWTC 1 cut(s) 543
PleI GAGTC 1 cut(s) 1165
PpsI GAGTC 1 cut(s) 1165
PshAI GACNNNNGTC 1 cut(s) 1191
PshBI ATTAAT 1 cut(s) 858
Psp1406I AACGTT 1 cut(s) 1182
Psp6I CCWGG 1 cut(s) 518
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 1 cut(s) 518
PspN4I GGNNCC 2 cut(s) 256, 791
PspXI VCTCGAGB 1 cut(s) 401
PsuI RGATCY 2 cut(s) 238, 872
RsaI GTAC 7 cut(s) 364, 370, 480, 609, 681, 804, 968
RsaNI GTAC 7 cut(s) 363, 369, 479, 608, 680, 803, 967
RseI CAYNNNNRTG 1 cut(s) 711
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 6 cut(s) 300, 600, 858, 1032, 1226, 1279
Sau3AI GATC 3 cut(s) 238, 872, 919
ScaI AGTACT 1 cut(s) 968
SchI GAGTC 1 cut(s) 1166
ScrFI CCNGG 2 cut(s) 83, 520
SduI GDGCHC 3 cut(s) 571, 811, 1176
SfaNI GCATC 1 cut(s) 444
Sfr274I CTCGAG 1 cut(s) 401
SgrDI CGTCGACG 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 401
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
SpeI ACTAGT 3 cut(s) 365, 827, 1190
Sse9I AATT 7 cut(s) 320, 328, 794, 855, 859, 1037, 1228
SsiI CCGC 8 cut(s) 44, 50, 56, 140, 173, 242, 258, 823
SspMI CTAG 5 cut(s) 236, 366, 828, 864, 1191
StyD4I CCNGG 2 cut(s) 81, 518
StyI CCWWGG 1 cut(s) 868
TaaI ACNGT 3 cut(s) 679, 908, 1167
TaiI ACGT 2 cut(s) 715, 1185
TaqI TCGA 2 cut(s) 216, 402
TasI AATT 7 cut(s) 320, 328, 794, 855, 859, 1037, 1228
TatI WGTACW 5 cut(s) 362, 368, 607, 679, 966
TauI GCSGC 5 cut(s) 47, 53, 59, 143, 175
TfiI GAWTC 1 cut(s) 543
Tru1I TTAA 6 cut(s) 300, 600, 858, 1032, 1226, 1279
Tru9I TTAA 6 cut(s) 300, 600, 858, 1032, 1226, 1279
TscAI CASTG 4 cut(s) 631, 675, 1172, 1266
TseFI GTSAC 1 cut(s) 273
TseI GCWGC 9 cut(s) 41, 47, 53, 59, 143, 221, 585, 820, 1241
Tsp45I GTSAC 1 cut(s) 273
TspDTI ATGAA 4 cut(s) 917, 974, 1050, 1251
TspGWI ACGGA 3 cut(s) 173, 213, 1074
TspRI CASTG 4 cut(s) 631, 675, 1172, 1266
VspI ATTAAT 1 cut(s) 858
XapI RAATTY 2 cut(s) 320, 1228
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
XhoI CTCGAG 1 cut(s) 401
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 5 cut(s) 236, 366, 828, 864, 1191
ZrmI AGTACT 1 cut(s) 968
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.