FvH4_4g16450

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002526
Physical Location & Seq
Reverse (-)
20397922 .. 20398830
909 bp
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UTR
Exon/CDS
Intron
FvH4_4g16450.t1

Sequence Viewer

Length: 378 bp
ATTATAATCCAAGTCCAACTAGACATTGATTGGTGCAGGTTTGGGGCAGTCAGCTTACAACTCGGTACCCAAATCTCTTCTCCTCTTCTCCCTATTGACTCTGCTTTTGCTCTTCCTCCTGTCTTCCTCTCCTCGCCGTCTGACTTCTCTTGGGCTCCGGCACTCCAACGCATCATGACTCGCGGTAACCAGAGAGAGAAGGATCGGGAGAGAGCTGCGGCTCGGGCCAAGGCTAGCAAGAACAAAGACGATGGATTGACCCCAGAACAGCGTCGAGAGAGGGATGCCAAAGCTCTTCAAGAAAAGTTGGCCAAGAAGGCTGACAAGAGTGCAGGGGCGGCCGGAGCAAACTCCTCCTCCGGAGGTAAAAGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

126

Amino Acids

13.51

Weight (kDa)

9.93

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SERF-like_N PF04419 59 - 92 1.4e-10 Small EDRK-rich factor 1/2-like, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5
Acc36I ACCTGC 1 cut(s) 27
Acc65I GGTACC 1 cut(s) 65
AccB1I GGYRCC 1 cut(s) 65
AccII CGCG 1 cut(s) 183
AccIII TCCGGA 1 cut(s) 359
AciI CCGC 3 cut(s) 183, 218, 338
AclWI GGATC 1 cut(s) 210
AcoI YGGCCR 2 cut(s) 309, 339
AfaI GTAC 1 cut(s) 67
AgsI TTSAA 1 cut(s) 299
AluBI AGCT 3 cut(s) 54, 215, 293
AluI AGCT 3 cut(s) 54, 215, 293
AlwI GGATC 1 cut(s) 210
Ama87I CYCGRG 1 cut(s) 222
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 3 cut(s) 225, 309, 339
ApeKI GCWGC 1 cut(s) 215
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
Asp718I GGTACC 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 225
AsuNHI GCTAGC 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 222
BalI TGGCCA 1 cut(s) 311
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 157
BbsI GAAGAC 1 cut(s) 115
BbvI GCAGC 1 cut(s) 202
BccI CCATC 1 cut(s) 245
BceAI ACGGC 1 cut(s) 121
BfaI CTAG 2 cut(s) 20, 234
BfuAI ACCTGC 1 cut(s) 27
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 3 cut(s) 216, 219, 339
BlsI GCNGC 3 cut(s) 217, 220, 340
BmeT110I CYCGRG 1 cut(s) 222
BmgT120I GGNCC 1 cut(s) 225
BmiI GGNNCC 2 cut(s) 67, 156
BmsI GCATC 2 cut(s) 180, 274
BmtI GCTAGC 1 cut(s) 237
BpiI GAAGAC 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
BseAI TCCGGA 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 1 cut(s) 289
BseRI GAGGAG 4 cut(s) 72, 121, 343, 346
BseX3I CGGCCG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 202
BsgI GTGCAG 2 cut(s) 55, 351
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 3 cut(s) 227, 311, 341
BshNI GGYRCC 1 cut(s) 65
BsiEI CGRYCG 1 cut(s) 342
BsiHKCI CYCGRG 1 cut(s) 222
BsiSI CCGG 3 cut(s) 158, 342, 360
BsnI GGCC 3 cut(s) 227, 311, 341
BsoBI CYCGRG 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 157
Bsp13I TCCGGA 1 cut(s) 359
Bsp143I GATC 1 cut(s) 202
BspACI CCGC 3 cut(s) 183, 218, 338
BspANI GGCC 3 cut(s) 227, 311, 341
BspEI TCCGGA 1 cut(s) 359
BspFNI CGCG 1 cut(s) 183
BspHI TCATGA 1 cut(s) 174
BspLI GGNNCC 2 cut(s) 67, 156
BspMI ACCTGC 1 cut(s) 27
BspOI GCTAGC 1 cut(s) 237
BspPI GGATC 1 cut(s) 210
BspQI GCTCTTC 2 cut(s) 117, 300
BspT107I GGYRCC 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 1 cut(s) 202
BssT1I CCWWGG 1 cut(s) 228
Bst6I CTCTTC 4 cut(s) 82, 90, 117, 300
BstC8I GCNNGC 1 cut(s) 235
BstEII GGTNACC 1 cut(s) 185
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 1 cut(s) 183
BstKTI GATC 1 cut(s) 205
BstMBI GATC 1 cut(s) 202
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 4 cut(s) 224, 317, 338, 344
BstPI GGTNACC 1 cut(s) 185
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 202
BstV2I GAAGAC 1 cut(s) 115
BstZI CGGCCG 1 cut(s) 339
BsuRI GGCC 3 cut(s) 227, 311, 341
BtsCI GGATG 1 cut(s) 289
BveI ACCTGC 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 235
CciI TCATGA 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 225
CseI GACGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 66
CviAII CATG 1 cut(s) 175
CviQI GTAC 1 cut(s) 66
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
EaeI YGGCCR 2 cut(s) 309, 339
EagI CGGCCG 1 cut(s) 339
Eam1104I CTCTTC 4 cut(s) 82, 90, 117, 300
EarI CTCTTC 4 cut(s) 82, 90, 117, 300
EclXI CGGCCG 1 cut(s) 339
Eco130I CCWWGG 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 157
Eco52I CGGCCG 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 222
Eco91I GGTNACC 1 cut(s) 185
EcoO65I GGTNACC 1 cut(s) 185
EcoT14I CCWWGG 1 cut(s) 228
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 1 cut(s) 178
FaiI YATR 2 cut(s) 5, 176
FatI CATG 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 216, 219, 339
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 3 cut(s) 216, 219, 339
FspBI CTAG 2 cut(s) 20, 234
GluI GCNGC 3 cut(s) 216, 219, 339
HaeIII GGCC 3 cut(s) 227, 311, 341
HapII CCGG 3 cut(s) 158, 342, 360
HgaI GACGC 1 cut(s) 260
Hin1II CATG 1 cut(s) 178
HinfI GANTC 2 cut(s) 98, 178
HpaII CCGG 3 cut(s) 158, 342, 360
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 5 cut(s) 175, 206, 275, 299, 360
Hpy99I CGWCG 1 cut(s) 276
HpyAV CCTTC 2 cut(s) 193, 310
HpyCH4V TGCA 2 cut(s) 36, 332
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 317, 338, 344
Hsp92II CATG 1 cut(s) 178
Kpn2I TCCGGA 1 cut(s) 359
KpnI GGTACC 1 cut(s) 69
Kzo9I GATC 1 cut(s) 202
LguI GCTCTTC 2 cut(s) 117, 300
LmnI GCTCC 2 cut(s) 160, 344
LpnPI CCDG 8 cut(s) 22, 132, 171, 203, 276, 318, 355, 373
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 2 cut(s) 180, 274
MaeI CTAG 2 cut(s) 20, 234
MaeIII GTNAC 1 cut(s) 185
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 5 cut(s) 69, 77, 104, 115, 287
MhlI GDGCHC 1 cut(s) 157
MlsI TGGCCA 1 cut(s) 311
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 2 cut(s) 92, 172
MmeI TCCRAC 2 cut(s) 40, 190
MnlI CCTC 8 cut(s) 93, 126, 137, 142, 273, 356, 364, 367
Mox20I TGGCCA 1 cut(s) 311
MroI TCCGGA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 311
Msp20I TGGCCA 1 cut(s) 311
MspI CCGG 3 cut(s) 158, 342, 360
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 4 cut(s) 224, 317, 338, 344
NdeII GATC 1 cut(s) 202
NheI GCTAGC 1 cut(s) 233
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 2 cut(s) 67, 156
PagI TCATGA 1 cut(s) 174
PciSI GCTCTTC 2 cut(s) 117, 300
PkrI GCNGC 3 cut(s) 217, 220, 340
PleI GAGTC 2 cut(s) 92, 172
PpsI GAGTC 2 cut(s) 92, 172
PsiI TTATAA 1 cut(s) 5
PspEI GGTNACC 1 cut(s) 185
PspN4I GGNNCC 2 cut(s) 67, 156
PspPI GGNCC 1 cut(s) 225
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
SapI GCTCTTC 2 cut(s) 117, 300
SatI GCNGC 3 cut(s) 216, 219, 339
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 1 cut(s) 225
SchI GAGTC 2 cut(s) 92, 172
SduI GDGCHC 1 cut(s) 157
SetI ASST 5 cut(s) 41, 56, 217, 295, 367
SfaNI GCATC 2 cut(s) 180, 274
SsiI CCGC 3 cut(s) 183, 218, 338
SspMI CTAG 2 cut(s) 20, 234
StyI CCWWGG 1 cut(s) 228
TaqI TCGA 1 cut(s) 274
TauI GCSGC 2 cut(s) 221, 341
TseI GCWGC 1 cut(s) 215
XspI CTAG 2 cut(s) 20, 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.