MD05G1174200.v1.1

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002526
Physical Location & Seq
Forward (+)
30239995 .. 30241347
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1174200.v1.1.491

Sequence Viewer

Length: 219 bp
ATGTCCCGCGGAAACCAGAGGGATCGAGACCGCGAAAGGGCTCAGGCCAGGTCTGGCGGCAACAAATCCAAGCCCAAAAATGACGGATTAACCCCTGAACAACGCCGCGAAAGGGACGCGAAAGCGCTGCAGGAGAAGGCGAAGAAGAAAGCAGCGGCGCAGCAGGCCGGAGGGATCAACGCCGGCGGCAAAAAATTAGATGGGATGGAGATAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

73

Amino Acids

7.89

Weight (kDa)

10.53

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SERF-like_N PF04419 1 - 36 1.4e-12 Small EDRK-rich factor 1/2-like, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 9, 33, 108, 119
AciI CCGC 7 cut(s) 7, 9, 31, 57, 106, 155, 186
AclWI GGATC 2 cut(s) 30, 182
AfeI AGCGCT 1 cut(s) 126
AfiI CCNNNNNNNGG 2 cut(s) 37, 112
AjnI CCWGG 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 2 cut(s) 30, 182
Aor51HI AGCGCT 1 cut(s) 126
AoxI GGCC 2 cut(s) 45, 165
ApeKI GCWGC 3 cut(s) 127, 152, 160
AspLEI GCGC 2 cut(s) 127, 160
BanII GRGCYC 1 cut(s) 43
BbvI GCAGC 3 cut(s) 114, 164, 172
BccI CCATC 2 cut(s) 194, 199
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 21
BfmI CTRYAG 1 cut(s) 128
BfoI RGCGCY 1 cut(s) 128
BisI GCNGC 7 cut(s) 58, 106, 128, 153, 156, 161, 187
BlsI GCNGC 7 cut(s) 59, 107, 129, 154, 157, 162, 188
Bme1390I CCNGG 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 49
Bpu10I CCTNAGC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 7
BsaXI ACNNNNNCTCC 1 cut(s) 200
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 112
Bse118I RCCGGY 1 cut(s) 182
BseBI CCWGG 1 cut(s) 49
BseDI CCNNGG 1 cut(s) 7
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 2 cut(s) 37, 112
BseMII CTCAG 1 cut(s) 56
BseXI GCAGC 3 cut(s) 114, 164, 172
Bsh1236I CGCG 4 cut(s) 9, 33, 108, 119
BshFI GGCC 2 cut(s) 47, 167
BsiSI CCGG 2 cut(s) 168, 183
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 37, 112
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 2 cut(s) 47, 167
Bso31I GGTCTC 1 cut(s) 21
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 2 cut(s) 22, 174
BspACI CCGC 7 cut(s) 7, 9, 31, 57, 106, 155, 186
BspANI GGCC 2 cut(s) 47, 167
BspCNI CTCAG 1 cut(s) 55
BspFNI CGCG 4 cut(s) 9, 33, 108, 119
BspMAI CTGCAG 1 cut(s) 132
BspPI GGATC 2 cut(s) 30, 182
BspTNI GGTCTC 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 7
BssMI GATC 2 cut(s) 22, 174
Bst2UI CCWGG 1 cut(s) 49
BstC8I GCNNGC 2 cut(s) 165, 184
BstDEI CTNAG 1 cut(s) 42
BstDSI CCRYGG 1 cut(s) 7
BstF5I GGATG 1 cut(s) 210
BstFNI CGCG 4 cut(s) 9, 33, 108, 119
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 2 cut(s) 127, 160
BstKTI GATC 2 cut(s) 25, 177
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 2 cut(s) 22, 174
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 47
BstSFI CTRYAG 1 cut(s) 128
BstUI CGCG 4 cut(s) 9, 33, 108, 119
BstV1I GCAGC 3 cut(s) 114, 164, 172
BsuRI GGCC 2 cut(s) 47, 167
BtgI CCRYGG 1 cut(s) 7
BtsCI GGATG 1 cut(s) 210
Cac8I GCNNGC 2 cut(s) 165, 184
CfoI GCGC 2 cut(s) 127, 160
Cfr10I RCCGGY 1 cut(s) 182
Cfr42I CCGCGG 1 cut(s) 10
CseI GACGC 1 cut(s) 125
CviJI RGCY 4 cut(s) 41, 47, 73, 167
CviKI_1 RGCY 4 cut(s) 41, 47, 73, 167
DdeI CTNAG 1 cut(s) 42
DpnI GATC 2 cut(s) 24, 176
DpnII GATC 2 cut(s) 22, 174
Eco24I GRGCYC 1 cut(s) 43
Eco31I GGTCTC 1 cut(s) 21
Eco47III AGCGCT 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 43
FaqI GGGAC 1 cut(s) 128
FauI CCCGC 1 cut(s) 14
Fnu4HI GCNGC 7 cut(s) 58, 106, 128, 153, 156, 161, 187
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 7 cut(s) 58, 106, 128, 153, 156, 161, 187
GlaI GCGC 2 cut(s) 126, 159
GluI GCNGC 7 cut(s) 58, 106, 128, 153, 156, 161, 187
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 2 cut(s) 47, 167
HapII CCGG 2 cut(s) 168, 183
HgaI GACGC 1 cut(s) 125
HhaI GCGC 2 cut(s) 127, 160
Hin6I GCGC 2 cut(s) 125, 158
HinP1I GCGC 2 cut(s) 125, 158
HpaII CCGG 2 cut(s) 168, 183
Hpy188III TCNNGA 1 cut(s) 26
HpyAV CCTTC 1 cut(s) 130
HpyCH4V TGCA 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 42
HspAI GCGC 2 cut(s) 125, 158
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
KspI CCGCGG 1 cut(s) 10
Kzo9I GATC 2 cut(s) 22, 174
Lsp1109I GCAGC 3 cut(s) 114, 164, 172
MalI GATC 2 cut(s) 24, 176
MboI GATC 2 cut(s) 22, 174
MboII GAAGA 2 cut(s) 154, 157
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 1 cut(s) 194
MnlI CCTC 2 cut(s) 12, 164
MreI CGCCGGCG 1 cut(s) 182
MroNI GCCGGC 1 cut(s) 182
MseI TTAA 1 cut(s) 89
MspA1I CMGCKG 2 cut(s) 9, 155
MspI CCGG 2 cut(s) 168, 183
MspR9I CCNGG 1 cut(s) 49
MvaI CCWGG 1 cut(s) 49
MvnI CGCG 4 cut(s) 9, 33, 108, 119
MwoI GCNNNNNNNGC 1 cut(s) 164
NaeI GCCGGC 1 cut(s) 184
NdeII GATC 2 cut(s) 22, 174
NgoMIV GCCGGC 1 cut(s) 182
PdiI GCCGGC 1 cut(s) 184
PkrI GCNGC 7 cut(s) 59, 107, 129, 154, 157, 162, 188
Psp6I CCWGG 1 cut(s) 47
PspGI CCWGG 1 cut(s) 47
PstI CTGCAG 1 cut(s) 132
SacII CCGCGG 1 cut(s) 10
SaqAI TTAA 1 cut(s) 89
SatI GCNGC 7 cut(s) 58, 106, 128, 153, 156, 161, 187
Sau3AI GATC 2 cut(s) 22, 174
ScrFI CCNGG 1 cut(s) 49
SduI GDGCHC 1 cut(s) 43
SetI ASST 1 cut(s) 53
SfcI CTRYAG 1 cut(s) 128
Sfr303I CCGCGG 1 cut(s) 10
SgrAI CRCCGGYG 1 cut(s) 182
SgrBI CCGCGG 1 cut(s) 10
Sse9I AATT 1 cut(s) 194
SsiI CCGC 7 cut(s) 7, 9, 31, 57, 106, 155, 186
StyD4I CCNGG 1 cut(s) 47
TaqI TCGA 1 cut(s) 25
TasI AATT 1 cut(s) 194
TauI GCSGC 4 cut(s) 60, 108, 158, 189
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TseI GCWGC 3 cut(s) 127, 152, 160
TspGWI ACGGA 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.