Prupe.8G201600_v2.0.a1

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0002526
Physical Location & Seq
Forward (+)
19021654 .. 19023369
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G201600.6

Sequence Viewer

Length: 468 bp
ATGATAGTGTTGATGAGAGAGGGAGAAGCAGCCGGAGTGGGAAGAAAGGGAACCACTGAAGAAAGCGGCCGAAAAACCGCGGCTTCTCGAGAGTTCCGTAGATTGTTTGGTTCGCGACAGCCCGTCCTTTTAAGTCATCAGCTCTCCTCGGACGCGACGTCAAAACAAGGCTTCTTCAGTGTCTCGCTCTCTTTGAGCTCAGAGCATAGATCAGTTGCAGGGGTTTATTGTGTTTGTGATTTTGTTTGGCTGTTTCAGCTTCACTCGCACAATTCAATAATGTCCCGCGGAAGCCAGAGAGAGCGAGACCGTGAAAGGGCTCAGGCCAGGTCTGGCGGGAAATCCAAGCAGCCCAAAAACGATGGATTAACCCCTGAACAACGGCGACAAAGGGACGCCAAAGCGCTGCAAGAAAAGGCGGCAAAGAAGGCGGCGCAGGCGGGAGGGGTCAGCGGCGGTAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

156

Amino Acids

16.96

Weight (kDa)

10.57

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 161
AccII CGCG 4 cut(s) 80, 115, 155, 288
AcoI YGGCCR 1 cut(s) 67
AcuI CTGAAG 2 cut(s) 78, 160
AcyI GRCGYC 2 cut(s) 158, 396
AfeI AGCGCT 1 cut(s) 405
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 1 cut(s) 276
AhdI GACNNNNNGTC 2 cut(s) 122, 157
AjnI CCWGG 1 cut(s) 326
AluBI AGCT 3 cut(s) 142, 198, 259
AluI AGCT 3 cut(s) 142, 198, 259
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 2 cut(s) 187, 300
Ama87I CYCGRG 1 cut(s) 87
Aor51HI AGCGCT 1 cut(s) 405
AoxI GGCC 2 cut(s) 67, 324
ApeKI GCWGC 3 cut(s) 29, 349, 406
AspLEI GCGC 2 cut(s) 406, 436
AvaI CYCGRG 1 cut(s) 87
BanII GRGCYC 2 cut(s) 200, 322
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 3 cut(s) 41, 361, 393
BccI CCATC 1 cut(s) 356
BceAI ACGGC 1 cut(s) 398
BciT130I CCWGG 1 cut(s) 328
BcoDI GTCTC 2 cut(s) 187, 300
BfoI RGCGCY 1 cut(s) 407
BisI GCNGC 8 cut(s) 30, 67, 81, 350, 407, 420, 432, 454
BlsI GCNGC 8 cut(s) 31, 68, 82, 351, 408, 421, 433, 455
Bme1390I CCNGG 1 cut(s) 328
BmeRI GACNNNNNGTC 2 cut(s) 122, 157
BmeT110I CYCGRG 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 328
Bpu10I CCTNAGC 1 cut(s) 321
BsaHI GRCGYC 2 cut(s) 158, 396
BsaI GGTCTC 1 cut(s) 300
BsaJI CCNNGG 3 cut(s) 78, 147, 286
Bsc4I CCNNNNNNNGG 1 cut(s) 316
BseBI CCWGG 1 cut(s) 328
BseDI CCNNGG 3 cut(s) 78, 147, 286
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 2 cut(s) 213, 335
BseRI GAGGAG 1 cut(s) 136
BseX3I CGGCCG 1 cut(s) 67
BseXI GCAGC 3 cut(s) 41, 361, 393
Bsh1236I CGCG 4 cut(s) 80, 115, 155, 288
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 2 cut(s) 69, 326
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 87
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 2 cut(s) 268, 407
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 2 cut(s) 187, 300
BsmFI GGGAC 2 cut(s) 268, 407
BsnI GGCC 2 cut(s) 69, 326
Bso31I GGTCTC 1 cut(s) 300
BsoBI CYCGRG 1 cut(s) 87
Bsp1286I GDGCHC 2 cut(s) 200, 322
Bsp143I GATC 1 cut(s) 209
Bsp68I TCGCGA 1 cut(s) 115
BspANI GGCC 2 cut(s) 69, 326
BspCNI CTCAG 2 cut(s) 212, 334
BspFNI CGCG 4 cut(s) 80, 115, 155, 288
BspLI GGNNCC 1 cut(s) 52
BspTNI GGTCTC 1 cut(s) 300
BssECI CCNNGG 3 cut(s) 78, 147, 286
BssMI GATC 1 cut(s) 209
BssNI GRCGYC 2 cut(s) 158, 396
Bst2UI CCWGG 1 cut(s) 328
Bst4CI ACNGT 1 cut(s) 311
BstACI GRCGYC 2 cut(s) 158, 396
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 2 cut(s) 199, 321
BstDSI CCRYGG 2 cut(s) 78, 286
BstFNI CGCG 4 cut(s) 80, 115, 155, 288
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 2 cut(s) 406, 436
BstKTI GATC 1 cut(s) 212
BstMAI GTCTC 2 cut(s) 187, 300
BstMBI GATC 1 cut(s) 209
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 4 cut(s) 256, 265, 428, 437
BstNI CCWGG 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 326
BstUI CGCG 4 cut(s) 80, 115, 155, 288
BstV1I GCAGC 3 cut(s) 41, 361, 393
BstZI CGGCCG 1 cut(s) 67
BsuRI GGCC 2 cut(s) 69, 326
BtgI CCRYGG 2 cut(s) 78, 286
BtsIMutI CAGTG 2 cut(s) 54, 184
BtuMI TCGCGA 1 cut(s) 115
Cac8I GCNNGC 1 cut(s) 438
CfoI GCGC 2 cut(s) 406, 436
Cfr42I CCGCGG 2 cut(s) 81, 289
CseI GACGC 2 cut(s) 161, 404
DdeI CTNAG 2 cut(s) 199, 321
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
DriI GACNNNNNGTC 2 cut(s) 122, 157
EaeI YGGCCR 1 cut(s) 67
EagI CGGCCG 1 cut(s) 67
Eam1105I GACNNNNNGTC 2 cut(s) 122, 157
Ecl136II GAGCTC 1 cut(s) 198
EclXI CGGCCG 1 cut(s) 67
Eco24I GRGCYC 2 cut(s) 200, 322
Eco31I GGTCTC 1 cut(s) 300
Eco47III AGCGCT 1 cut(s) 405
Eco52I CGGCCG 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 198
Eco57I CTGAAG 2 cut(s) 78, 160
Eco88I CYCGRG 1 cut(s) 87
EcoICRI GAGCTC 1 cut(s) 198
EcoRII CCWGG 1 cut(s) 326
EcoT38I GRGCYC 2 cut(s) 200, 322
FaiI YATR 1 cut(s) 207
FaqI GGGAC 2 cut(s) 268, 407
FauI CCCGC 3 cut(s) 293, 329, 433
Fnu4HI GCNGC 8 cut(s) 30, 67, 81, 350, 407, 420, 432, 454
FriOI GRGCYC 2 cut(s) 200, 322
Fsp4HI GCNGC 8 cut(s) 30, 67, 81, 350, 407, 420, 432, 454
GlaI GCGC 2 cut(s) 405, 435
GluI GCNGC 8 cut(s) 30, 67, 81, 350, 407, 420, 432, 454
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 2 cut(s) 69, 326
HapII CCGG 1 cut(s) 33
HgaI GACGC 2 cut(s) 161, 404
HhaI GCGC 2 cut(s) 406, 436
Hin1I GRCGYC 2 cut(s) 158, 396
Hin6I GCGC 2 cut(s) 404, 434
HinP1I GCGC 2 cut(s) 404, 434
HpaII CCGG 1 cut(s) 33
Hpy188I TCNGA 2 cut(s) 151, 202
Hpy188III TCNNGA 3 cut(s) 87, 89, 114
Hpy99I CGWCG 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 421
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 2 cut(s) 218, 409
HpyF10VI GCNNNNNNNGC 4 cut(s) 256, 265, 428, 437
HpyF3I CTNAG 2 cut(s) 199, 321
HpySE526I ACGT 1 cut(s) 158
Hsp92I GRCGYC 2 cut(s) 158, 396
HspAI GCGC 2 cut(s) 404, 434
KspI CCGCGG 2 cut(s) 81, 289
Kzo9I GATC 1 cut(s) 209
LpnPI CCDG 9 cut(s) 46, 204, 308, 308, 313, 318, 340, 387, 422
Lsp1109I GCAGC 3 cut(s) 41, 361, 393
MaeII ACGT 1 cut(s) 158
MalI GATC 1 cut(s) 211
MboI GATC 1 cut(s) 209
MboII GAAGA 3 cut(s) 54, 71, 166
MhlI GDGCHC 2 cut(s) 200, 322
MluCI AATT 2 cut(s) 271, 463
MnlI CCTC 3 cut(s) 13, 157, 437
MseI TTAA 2 cut(s) 131, 368
MspA1I CMGCKG 3 cut(s) 80, 288, 453
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 328
MvaI CCWGG 1 cut(s) 328
MvnI CGCG 4 cut(s) 80, 115, 155, 288
MwoI GCNNNNNNNGC 4 cut(s) 256, 265, 428, 437
NdeII GATC 1 cut(s) 209
NlaIV GGNNCC 1 cut(s) 52
NruI TCGCGA 1 cut(s) 115
PaeR7I CTCGAG 1 cut(s) 87
PcsI WCGNNNNNNNCGW 2 cut(s) 94, 155
PkrI GCNGC 8 cut(s) 31, 68, 82, 351, 408, 421, 433, 455
Psp124BI GAGCTC 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 326
PspGI CCWGG 1 cut(s) 326
PspN4I GGNNCC 1 cut(s) 52
RruI TCGCGA 1 cut(s) 115
SacI GAGCTC 1 cut(s) 200
SacII CCGCGG 2 cut(s) 81, 289
SaqAI TTAA 2 cut(s) 131, 368
SatI GCNGC 8 cut(s) 30, 67, 81, 350, 407, 420, 432, 454
Sau3AI GATC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 328
SduI GDGCHC 2 cut(s) 200, 322
SetI ASST 5 cut(s) 144, 161, 200, 261, 332
Sfr274I CTCGAG 1 cut(s) 87
Sfr303I CCGCGG 2 cut(s) 81, 289
SgrBI CCGCGG 2 cut(s) 81, 289
SlaI CTCGAG 1 cut(s) 87
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 2 cut(s) 271, 463
SstI GAGCTC 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 326
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 1 cut(s) 161
TaqI TCGA 1 cut(s) 88
TasI AATT 2 cut(s) 271, 463
TauI GCSGC 5 cut(s) 69, 83, 422, 434, 456
Tru1I TTAA 2 cut(s) 131, 368
Tru9I TTAA 2 cut(s) 131, 368
TscAI CASTG 2 cut(s) 61, 184
TseI GCWGC 3 cut(s) 29, 349, 406
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 2 cut(s) 61, 184
XhoI CTCGAG 1 cut(s) 87
ZraI GACGTC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.