RLG00000008089

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002526
Physical Location & Seq
Reverse (-)
25473668 .. 25477981
4314 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008089

Sequence Viewer

Length: 471 bp
ATGGCTAGGCTCTCATGGATTTCCATTACTTTCTTTGTCATCCTTGCCTTCGCAATCAATACTTTGGCTCAATGGGATATCGATCAAAGTCCTGCTCCTGCTGTTCAAATTTTTCCTATTGCGCGGCAGCGTGTGATTTTCGGTGTTCAGCAACTTCACACAAGAACCCATGTATATTCTTTTGCAATATGTGTTGTCAAAAATGTCTGTGTGTCCCATCGGGCACATATGGACACAAAGAAGAATGTCCATGCTACAATAACTGGAAAACCAAGGAAGGAGAGAGAGAAGGATCGGGAGAGAGCTCAGGCTCGGGGCGGCAAGGGTAGCAAGAACAAAGACGATGGCTTGACCCCAGAACAGCGCCGAGAGAGGGATGCCAAAGCACTCCAAGAAAAGCTGGCCAAGAAGGCTGATAAGGGTACAGGGGCGGCCGGAGCGAACTCCTCCTCTGGAGGTAAAAGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

157

Amino Acids

17.25

Weight (kDa)

10.36

Isoelectric Point (pI)

27.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 124
AciI CCGC 3 cut(s) 124, 318, 431
AclWI GGATC 1 cut(s) 300
AcoI YGGCCR 2 cut(s) 402, 432
AcsI RAATTY 1 cut(s) 108
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 1 cut(s) 373
AgsI TTSAA 1 cut(s) 107
AluBI AGCT 2 cut(s) 305, 400
AluI AGCT 2 cut(s) 305, 400
Alw21I GWGCWC 1 cut(s) 307
AlwI GGATC 1 cut(s) 300
Ama87I CYCGRG 1 cut(s) 312
AoxI GGCC 2 cut(s) 402, 432
ApeKI GCWGC 1 cut(s) 127
ApoI RAATTY 1 cut(s) 108
AspLEI GCGC 2 cut(s) 124, 366
AvaI CYCGRG 1 cut(s) 312
BaeGI GKGCMC 1 cut(s) 226
BalI TGGCCA 1 cut(s) 404
BanII GRGCYC 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 139
BccI CCATC 2 cut(s) 225, 338
BfaI CTAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 367
BglI GCCNNNNNGGC 1 cut(s) 410
BisI GCNGC 4 cut(s) 125, 128, 319, 432
BlsI GCNGC 4 cut(s) 126, 129, 320, 433
BmeT110I CYCGRG 1 cut(s) 312
BmsI GCATC 1 cut(s) 367
Bpu10I CCTNAGC 1 cut(s) 306
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 272
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse1I ACTGG 1 cut(s) 268
Bse8I GATNNNNATC 1 cut(s) 81
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 272
BseGI GGATG 2 cut(s) 39, 382
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 373
BseMII CTCAG 1 cut(s) 320
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 2 cut(s) 436, 439
BseSI GKGCMC 1 cut(s) 226
BseX3I CGGCCG 1 cut(s) 432
BseXI GCAGC 1 cut(s) 139
Bsh1236I CGCG 1 cut(s) 124
Bsh1285I CGRYCG 1 cut(s) 435
BshFI GGCC 2 cut(s) 404, 434
BshVI ATCGAT 1 cut(s) 81
BsiEI CGRYCG 1 cut(s) 435
BsiHKAI GWGCWC 1 cut(s) 307
BsiHKCI CYCGRG 1 cut(s) 312
BsiSI CCGG 1 cut(s) 435
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 1 cut(s) 373
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 2 cut(s) 404, 434
BsoBI CYCGRG 1 cut(s) 312
Bsp1286I GDGCHC 2 cut(s) 226, 307
Bsp143I GATC 2 cut(s) 82, 292
BspACI CCGC 3 cut(s) 124, 318, 431
BspANI GGCC 2 cut(s) 404, 434
BspCNI CTCAG 1 cut(s) 319
BspDI ATCGAT 1 cut(s) 81
BspFNI CGCG 1 cut(s) 124
BspPI GGATC 1 cut(s) 300
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 272
BssMI GATC 2 cut(s) 82, 292
BssT1I CCWWGG 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 39, 382
BstFNI CGCG 1 cut(s) 124
BstH2I RGCGCY 1 cut(s) 367
BstHHI GCGC 2 cut(s) 124, 366
BstKTI GATC 2 cut(s) 85, 295
BstMBI GATC 2 cut(s) 82, 292
BstMCI CGRYCG 1 cut(s) 435
BstMWI GCNNNNNNNGC 3 cut(s) 327, 410, 437
BstSLI GKGCMC 1 cut(s) 226
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 1 cut(s) 139
BstZI CGGCCG 1 cut(s) 432
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 2 cut(s) 404, 434
BsuTUI ATCGAT 1 cut(s) 81
BtsCI GGATG 2 cut(s) 39, 382
Cac8I GCNNGC 1 cut(s) 402
CfoI GCGC 2 cut(s) 124, 366
ClaI ATCGAT 1 cut(s) 81
Csp6I GTAC 1 cut(s) 423
CviAII CATG 3 cut(s) 15, 170, 251
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 1 cut(s) 306
DpnI GATC 2 cut(s) 84, 294
DpnII GATC 2 cut(s) 82, 292
EaeI YGGCCR 2 cut(s) 402, 432
EagI CGGCCG 1 cut(s) 432
Ecl136II GAGCTC 1 cut(s) 305
EclXI CGGCCG 1 cut(s) 432
Eco130I CCWWGG 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 307
Eco32I GATATC 1 cut(s) 79
Eco52I CGGCCG 1 cut(s) 432
Eco53kI GAGCTC 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 312
EcoICRI GAGCTC 1 cut(s) 305
EcoRV GATATC 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 272
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 272
FaeI CATG 3 cut(s) 18, 173, 254
FaiI YATR 7 cut(s) 16, 171, 175, 190, 228, 230, 252
FaqI GGGAC 1 cut(s) 199
FatI CATG 3 cut(s) 14, 169, 250
FauNDI CATATG 1 cut(s) 228
Fnu4HI GCNGC 4 cut(s) 125, 128, 319, 432
FokI GGATG 2 cut(s) 26, 389
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 4 cut(s) 125, 128, 319, 432
FspBI CTAG 1 cut(s) 6
GlaI GCGC 2 cut(s) 123, 365
GluI GCNGC 4 cut(s) 125, 128, 319, 432
HaeII RGCGCY 1 cut(s) 367
HaeIII GGCC 2 cut(s) 404, 434
HapII CCGG 1 cut(s) 435
HhaI GCGC 2 cut(s) 124, 366
Hin1II CATG 3 cut(s) 18, 173, 254
Hin6I GCGC 2 cut(s) 122, 364
HinP1I GCGC 2 cut(s) 122, 364
HpaII CCGG 1 cut(s) 435
Hpy188III TCNNGA 2 cut(s) 296, 453
HpyAV CCTTC 4 cut(s) 58, 271, 283, 403
HpyCH4V TGCA 1 cut(s) 185
HpyF10VI GCNNNNNNNGC 3 cut(s) 327, 410, 437
HpyF3I CTNAG 1 cut(s) 306
Hsp92II CATG 3 cut(s) 18, 173, 254
HspAI GCGC 2 cut(s) 122, 364
Kzo9I GATC 2 cut(s) 82, 292
LmnI GCTCC 2 cut(s) 100, 437
LpnPI CCDG 9 cut(s) 105, 111, 249, 293, 369, 386, 411, 438, 448
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 1 cut(s) 367
MaeI CTAG 1 cut(s) 6
MalI GATC 2 cut(s) 84, 294
MboI GATC 2 cut(s) 82, 292
MboII GAAGA 1 cut(s) 253
MhlI GDGCHC 2 cut(s) 226, 307
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 1 cut(s) 108
MluNI TGGCCA 1 cut(s) 404
MnlI CCTC 4 cut(s) 366, 449, 457, 460
Mox20I TGGCCA 1 cut(s) 404
MscI TGGCCA 1 cut(s) 404
Msp20I TGGCCA 1 cut(s) 404
MspI CCGG 1 cut(s) 435
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 3 cut(s) 327, 410, 437
NdeI CATATG 1 cut(s) 228
NdeII GATC 2 cut(s) 82, 292
NlaIII CATG 3 cut(s) 18, 173, 254
NmeAIII GCCGAG 1 cut(s) 392
PkrI GCNGC 4 cut(s) 126, 129, 320, 433
Psp124BI GAGCTC 1 cut(s) 307
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
SacI GAGCTC 1 cut(s) 307
SatI GCNGC 4 cut(s) 125, 128, 319, 432
Sau3AI GATC 2 cut(s) 82, 292
SduI GDGCHC 2 cut(s) 226, 307
SetI ASST 3 cut(s) 307, 402, 460
SfaNI GCATC 1 cut(s) 367
Sse9I AATT 1 cut(s) 108
SsiI CCGC 3 cut(s) 124, 318, 431
SspMI CTAG 1 cut(s) 6
SstI GAGCTC 1 cut(s) 307
StyI CCWWGG 1 cut(s) 272
TaqI TCGA 1 cut(s) 81
TasI AATT 1 cut(s) 108
TauI GCSGC 3 cut(s) 127, 321, 434
TseI GCWGC 1 cut(s) 127
XapI RAATTY 1 cut(s) 108
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.