Rh6BG277400

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002526
Physical Location & Seq
Forward (+)
49809119 .. 49816453
7335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG277400.1

Sequence Viewer

Length: 336 bp
ATGGAAGGTTCTGAACAACTGGTCCTTTATATACCCACCCACCTCTTCTCACTCGCGGACACATTCAACCTTGCTCTGATAGACGAAGGATTACACCCATCTGCGCTCACATCGTCAGTCAGCGAACGCGGTAACCAGAGAGACCGAGATCGCGAAAGGGCTCAGGCCAGGTCCGGCAACAAGACCAAGCAGCCCAAAACCGATGGCTTAACCCCTGAACAACGCCGAGAAAGGGACGCCAAGGCGCTGCAGGAAAAGGCGGCAAAGAAGGCGGCGCAGGCGCAGGCGGCCGGAGGGAACAAAGCGGGAGGAATCAATGCCGGCGGCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

111

Amino Acids

11.93

Weight (kDa)

9.75

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SERF-like_N PF04419 43 - 76 6.1e-08 Small EDRK-rich factor 1/2-like, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 56, 129, 153
AciI CCGC 7 cut(s) 56, 129, 260, 272, 287, 305, 324
AcoI YGGCCR 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 237
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 1 cut(s) 67
AjnI CCWGG 1 cut(s) 167
AloI GAACNNNNNNTCC 1 cut(s) 38
Alw26I GTCTC 1 cut(s) 135
AoxI GGCC 2 cut(s) 165, 288
ApeKI GCWGC 2 cut(s) 190, 247
AspLEI GCGC 4 cut(s) 106, 247, 277, 283
AspS9I GGNCC 2 cut(s) 22, 171
AvaII GGWCC 2 cut(s) 22, 171
BanII GRGCYC 1 cut(s) 163
BbvI GCAGC 2 cut(s) 202, 234
BccI CCATC 2 cut(s) 106, 197
BciT130I CCWGG 1 cut(s) 169
BcoDI GTCTC 1 cut(s) 135
BfmI CTRYAG 1 cut(s) 248
BfoI RGCGCY 1 cut(s) 248
BisI GCNGC 6 cut(s) 191, 248, 261, 273, 288, 325
BlsI GCNGC 6 cut(s) 192, 249, 262, 274, 289, 326
Bme1390I CCNGG 1 cut(s) 169
Bme18I GGWCC 2 cut(s) 22, 171
BmgT120I GGNCC 2 cut(s) 22, 171
BmrFI CCNGG 1 cut(s) 169
Bpu10I CCTNAGC 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 240
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 24
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 240
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 1 cut(s) 24
BseX3I CGGCCG 1 cut(s) 288
BseXI GCAGC 2 cut(s) 202, 234
Bsh1236I CGCG 3 cut(s) 56, 129, 153
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 2 cut(s) 167, 290
BsiEI CGRYCG 1 cut(s) 291
BsiSI CCGG 3 cut(s) 174, 291, 321
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 2 cut(s) 167, 290
Bso31I GGTCTC 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 1 cut(s) 148
Bsp68I TCGCGA 1 cut(s) 153
BspACI CCGC 7 cut(s) 56, 129, 260, 272, 287, 305, 324
BspANI GGCC 2 cut(s) 167, 290
BspCNI CTCAG 1 cut(s) 175
BspFNI CGCG 3 cut(s) 56, 129, 153
BspMAI CTGCAG 1 cut(s) 252
BspTNI GGTCTC 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 1 cut(s) 24
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 1 cut(s) 148
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 169
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 237
BstC8I GCNNGC 3 cut(s) 279, 285, 322
BstDEI CTNAG 1 cut(s) 162
BstEII GGTNACC 1 cut(s) 131
BstFNI CGCG 3 cut(s) 56, 129, 153
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 4 cut(s) 106, 247, 277, 283
BstKTI GATC 1 cut(s) 151
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 1 cut(s) 148
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 3 cut(s) 269, 278, 287
BstNI CCWGG 1 cut(s) 169
BstPI GGTNACC 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 248
BstUI CGCG 3 cut(s) 56, 129, 153
BstV1I GCAGC 2 cut(s) 202, 234
BstZI CGGCCG 1 cut(s) 288
BsuRI GGCC 2 cut(s) 167, 290
BtuMI TCGCGA 1 cut(s) 153
Cac8I GCNNGC 3 cut(s) 279, 285, 322
CfoI GCGC 4 cut(s) 106, 247, 277, 283
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 22, 171
CseI GACGC 1 cut(s) 245
CviJI RGCY 5 cut(s) 161, 167, 193, 207, 290
CviKI_1 RGCY 5 cut(s) 161, 167, 193, 207, 290
DdeI CTNAG 1 cut(s) 162
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 288
EagI CGGCCG 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
EclXI CGGCCG 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 163
Eco31I GGTCTC 1 cut(s) 135
Eco47I GGWCC 2 cut(s) 22, 171
Eco52I CGGCCG 1 cut(s) 288
Eco91I GGTNACC 1 cut(s) 131
EcoO65I GGTNACC 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 167
EcoT14I CCWWGG 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 240
FaiI YATR 2 cut(s) 30, 32
FaqI GGGAC 1 cut(s) 248
FauI CCCGC 1 cut(s) 298
Fnu4HI GCNGC 6 cut(s) 191, 248, 261, 273, 288, 325
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 6 cut(s) 191, 248, 261, 273, 288, 325
GlaI GCGC 4 cut(s) 105, 246, 276, 282
GluI GCNGC 6 cut(s) 191, 248, 261, 273, 288, 325
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 2 cut(s) 167, 290
HapII CCGG 3 cut(s) 174, 291, 321
HgaI GACGC 1 cut(s) 245
HhaI GCGC 4 cut(s) 106, 247, 277, 283
Hin1I GRCGYC 1 cut(s) 237
Hin6I GCGC 4 cut(s) 104, 245, 275, 281
HinP1I GCGC 4 cut(s) 104, 245, 275, 281
HinfI GANTC 1 cut(s) 312
HpaII CCGG 3 cut(s) 174, 291, 321
Hpy188I TCNGA 2 cut(s) 13, 78
Hpy188III TCNNGA 1 cut(s) 152
HpyAV CCTTC 2 cut(s) 80, 262
HpyCH4V TGCA 1 cut(s) 250
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 278, 287
HpyF3I CTNAG 1 cut(s) 162
Hsp92I GRCGYC 1 cut(s) 237
HspAI GCGC 4 cut(s) 104, 245, 275, 281
KroI GCCGGC 1 cut(s) 320
KroNI GCCGGC 1 cut(s) 322
Kzo9I GATC 1 cut(s) 148
Lsp1109I GCAGC 2 cut(s) 202, 234
MaeIII GTNAC 1 cut(s) 131
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 163
MnlI CCTC 3 cut(s) 53, 287, 302
MroNI GCCGGC 1 cut(s) 320
MseI TTAA 1 cut(s) 209
MspI CCGG 3 cut(s) 174, 291, 321
MspR9I CCNGG 1 cut(s) 169
MvaI CCWGG 1 cut(s) 169
MvnI CGCG 3 cut(s) 56, 129, 153
MwoI GCNNNNNNNGC 3 cut(s) 269, 278, 287
NaeI GCCGGC 1 cut(s) 322
NdeII GATC 1 cut(s) 148
NgoMIV GCCGGC 1 cut(s) 320
NmeAIII GCCGAG 1 cut(s) 251
NruI TCGCGA 1 cut(s) 153
PdiI GCCGGC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 312
PkrI GCNGC 6 cut(s) 192, 249, 262, 274, 289, 326
Psp6I CCWGG 1 cut(s) 167
PspEI GGTNACC 1 cut(s) 131
PspGI CCWGG 1 cut(s) 167
PspPI GGNCC 2 cut(s) 22, 171
PstI CTGCAG 1 cut(s) 252
RruI TCGCGA 1 cut(s) 153
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 6 cut(s) 191, 248, 261, 273, 288, 325
Sau3AI GATC 1 cut(s) 148
Sau96I GGNCC 2 cut(s) 22, 171
ScrFI CCNGG 1 cut(s) 169
SduI GDGCHC 1 cut(s) 163
SetI ASST 4 cut(s) 10, 45, 72, 173
SfcI CTRYAG 1 cut(s) 248
SinI GGWCC 2 cut(s) 22, 171
SsiI CCGC 7 cut(s) 56, 129, 260, 272, 287, 305, 324
StyD4I CCNGG 1 cut(s) 167
StyI CCWWGG 1 cut(s) 240
TaqII GACCGA 1 cut(s) 159
TauI GCSGC 4 cut(s) 263, 275, 290, 327
TfiI GAWTC 1 cut(s) 312
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TseI GCWGC 2 cut(s) 190, 247
VpaK11BI GGWCC 2 cut(s) 22, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.