MD04G1009100.v1.1

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002526
Physical Location & Seq
Reverse (-)
1057217 .. 1058059
843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1009100.v1.1.491

Sequence Viewer

Length: 192 bp
ATGACTCATATCCTTTCTTCTCGTGACCGGGAGAGAGCCGCCGCCCGGGCCAAGGCTGGGAAGAACAAAGACGACGGCTTGACGCCGGAGCAGCGCCGAGAGAGGGATGCGAAAGCGCTGCAAGAAAAGCAGGCGAAGAAGGCTGCGGGGGCGGCGGGAGGGAATTCCTCCGGAGGTAATAAAACGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

64

Amino Acids

6.69

Weight (kDa)

10.49

Isoelectric Point (pI)

60.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 5 cut(s) 39, 42, 146, 152, 155
AcsI RAATTY 1 cut(s) 163
AcyI GRCGYC 1 cut(s) 83
AfeI AGCGCT 1 cut(s) 117
AfiI CCNNNNNNNGG 4 cut(s) 45, 52, 57, 103
Ama87I CYCGRG 1 cut(s) 45
Aor13HI TCCGGA 1 cut(s) 170
Aor51HI AGCGCT 1 cut(s) 117
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 3 cut(s) 91, 118, 143
ApoI RAATTY 1 cut(s) 163
AspLEI GCGC 2 cut(s) 96, 118
AspS9I GGNCC 1 cut(s) 48
AsuC2I CCSGG 3 cut(s) 29, 46, 47
AvaI CYCGRG 1 cut(s) 45
BauI CACGAG 1 cut(s) 21
BbvI GCAGC 3 cut(s) 103, 105, 130
BceAI ACGGC 1 cut(s) 91
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BcnI CCSGG 3 cut(s) 29, 46, 47
BfoI RGCGCY 2 cut(s) 97, 119
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 6 cut(s) 39, 42, 92, 119, 144, 153
BlsI GCNGC 6 cut(s) 40, 43, 93, 120, 145, 154
Bme1390I CCNGG 3 cut(s) 29, 46, 47
BmeT110I CYCGRG 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 48
BmrFI CCNGG 3 cut(s) 29, 46, 47
BmsI GCATC 1 cut(s) 97
BpuMI CCSGG 3 cut(s) 29, 46, 47
BsaHI GRCGYC 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 45, 51
BsaWI WCCGGW 1 cut(s) 170
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 52, 57, 103
BseAI TCCGGA 1 cut(s) 170
BseDI CCNNGG 2 cut(s) 45, 51
BseGI GGATG 1 cut(s) 112
BseLI CCNNNNNNNGG 4 cut(s) 45, 52, 57, 103
BseXI GCAGC 3 cut(s) 103, 105, 130
BseYI CCCAGC 1 cut(s) 56
BshFI GGCC 1 cut(s) 50
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 4 cut(s) 28, 46, 86, 171
BslI CCNNNNNNNGG 4 cut(s) 45, 52, 57, 103
BsnI GGCC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 45
Bsp13I TCCGGA 1 cut(s) 170
BspACI CCGC 5 cut(s) 39, 42, 146, 152, 155
BspANI GGCC 1 cut(s) 50
BspEI TCCGGA 1 cut(s) 170
BssECI CCNNGG 2 cut(s) 45, 51
BssNI GRCGYC 1 cut(s) 83
BssSI CACGAG 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 132
BstF5I GGATG 1 cut(s) 112
BstH2I RGCGCY 2 cut(s) 97, 119
BstHHI GCGC 2 cut(s) 96, 118
BstMWI GCNNNNNNNGC 6 cut(s) 47, 91, 127, 140, 149, 152
BstSCI CCNGG 3 cut(s) 27, 44, 45
BstV1I GCAGC 3 cut(s) 103, 105, 130
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 132
CfoI GCGC 2 cut(s) 96, 118
Cfr13I GGNCC 1 cut(s) 48
Cfr9I CCCGGG 1 cut(s) 45
CseI GACGC 1 cut(s) 91
CviJI RGCY 5 cut(s) 38, 50, 56, 78, 143
CviKI_1 RGCY 5 cut(s) 38, 50, 56, 78, 143
Eco130I CCWWGG 1 cut(s) 51
Eco47III AGCGCT 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 45
EcoRI GAATTC 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
FaiI YATR 1 cut(s) 9
FauI CCCGC 2 cut(s) 139, 148
Fnu4HI GCNGC 6 cut(s) 39, 42, 92, 119, 144, 153
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 6 cut(s) 39, 42, 92, 119, 144, 153
GlaI GCGC 2 cut(s) 95, 117
GluI GCNGC 6 cut(s) 39, 42, 92, 119, 144, 153
GsaI CCCAGC 1 cut(s) 60
HaeII RGCGCY 2 cut(s) 97, 119
HaeIII GGCC 1 cut(s) 50
HapII CCGG 4 cut(s) 28, 46, 86, 171
HgaI GACGC 1 cut(s) 91
HhaI GCGC 2 cut(s) 96, 118
Hin1I GRCGYC 1 cut(s) 83
Hin6I GCGC 2 cut(s) 94, 116
HinP1I GCGC 2 cut(s) 94, 116
HinfI GANTC 1 cut(s) 4
HpaII CCGG 4 cut(s) 28, 46, 86, 171
Hpy188III TCNNGA 2 cut(s) 23, 171
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 133
HpyCH4V TGCA 1 cut(s) 121
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 91, 127, 140, 149, 152
Hsp92I GRCGYC 1 cut(s) 83
HspAI GCGC 2 cut(s) 94, 116
Kpn2I TCCGGA 1 cut(s) 170
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 6 cut(s) 41, 42, 59, 99, 116, 184
Lsp1109I GCAGC 3 cut(s) 103, 105, 130
LweI GCATC 1 cut(s) 97
MaeIII GTNAC 1 cut(s) 23
MboII GAAGA 3 cut(s) 9, 73, 148
MluCI AATT 1 cut(s) 163
MnlI CCTC 4 cut(s) 96, 152, 167, 178
MroI TCCGGA 1 cut(s) 170
MspI CCGG 4 cut(s) 28, 46, 86, 171
MspR9I CCNGG 3 cut(s) 29, 46, 47
MwoI GCNNNNNNNGC 6 cut(s) 47, 91, 127, 140, 149, 152
NciI CCSGG 3 cut(s) 29, 46, 47
NmeAIII GCCGAG 1 cut(s) 122
NmuCI GTSAC 1 cut(s) 23
PkrI GCNGC 6 cut(s) 40, 43, 93, 120, 145, 154
PspFI CCCAGC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 48
SatI GCNGC 6 cut(s) 39, 42, 92, 119, 144, 153
Sau96I GGNCC 1 cut(s) 48
ScrFI CCNGG 3 cut(s) 29, 46, 47
SetI ASST 1 cut(s) 178
SfaNI GCATC 1 cut(s) 97
SmaI CCCGGG 1 cut(s) 47
SrfI GCCCGGGC 1 cut(s) 47
Sse9I AATT 1 cut(s) 163
SsiI CCGC 5 cut(s) 39, 42, 146, 152, 155
StyD4I CCNGG 3 cut(s) 27, 44, 45
StyI CCWWGG 1 cut(s) 51
TasI AATT 1 cut(s) 163
TauI GCSGC 3 cut(s) 41, 44, 155
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 3 cut(s) 91, 118, 143
Tsp45I GTSAC 1 cut(s) 23
TspMI CCCGGG 1 cut(s) 45
XapI RAATTY 1 cut(s) 163
XmaI CCCGGG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.