Rw6G023830

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002526
Physical Location & Seq
Forward (+)
46469681 .. 46470093
413 bp
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UTR
Exon/CDS
Intron
Rw6G023830.1

Sequence Viewer

Length: 258 bp
ATGAATTTCTCGGCAATCGAACGATTAATTGGGGAAAATTTAGGCGAACGCGGTAACCAGAGAGACCGAGATCGCGAAAGGGCTCAGGCCAGGTCCGGCAACAAGACCAAGCAGCCCAAAACCGATGGATTAACCCCTGAACAACGCCGAGAAAGGGACGCCAAGGCGCTGCAGGAAAAGGAGGCAAAGAAAGCGGCGCAGGCGCAGGCGGCCGGAGGGAACAAAGCGGGAGGAATCAATGCCGGCGGCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

85

Amino Acids

9.23

Weight (kDa)

10.33

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SERF-like_N PF04419 17 - 50 3.9e-08 Small EDRK-rich factor 1/2-like, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 51, 75
AciI CCGC 5 cut(s) 51, 194, 209, 227, 246
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 2 cut(s) 4, 37
AcyI GRCGYC 1 cut(s) 159
AfiI CCNNNNNNNGG 1 cut(s) 154
AjnI CCWGG 1 cut(s) 89
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
Alw26I GTCTC 1 cut(s) 57
AoxI GGCC 2 cut(s) 87, 210
ApeKI GCWGC 2 cut(s) 112, 169
ApoI RAATTY 2 cut(s) 4, 37
AseI ATTAAT 1 cut(s) 26
AspLEI GCGC 3 cut(s) 169, 199, 205
AspS9I GGNCC 1 cut(s) 93
AvaII GGWCC 1 cut(s) 93
BanII GRGCYC 1 cut(s) 85
BbvI GCAGC 2 cut(s) 124, 156
BccI CCATC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 170
BfoI RGCGCY 1 cut(s) 170
BisI GCNGC 5 cut(s) 113, 170, 195, 210, 247
BlsI GCNGC 5 cut(s) 114, 171, 196, 211, 248
Bme1390I CCNGG 1 cut(s) 91
Bme18I GGWCC 1 cut(s) 93
BmgT120I GGNCC 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 91
Bpu10I CCTNAGC 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse118I RCCGGY 1 cut(s) 242
BseBI CCWGG 1 cut(s) 91
BseDI CCNNGG 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMII CTCAG 1 cut(s) 98
BseX3I CGGCCG 1 cut(s) 210
BseXI GCAGC 2 cut(s) 124, 156
Bsh1236I CGCG 2 cut(s) 51, 75
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 2 cut(s) 89, 212
BsiEI CGRYCG 1 cut(s) 213
BsiSI CCGG 3 cut(s) 96, 213, 243
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 2 cut(s) 89, 212
Bso31I GGTCTC 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 1 cut(s) 70
Bsp68I TCGCGA 1 cut(s) 75
BspACI CCGC 5 cut(s) 51, 194, 209, 227, 246
BspANI GGCC 2 cut(s) 89, 212
BspCNI CTCAG 1 cut(s) 97
BspFNI CGCG 2 cut(s) 51, 75
BspMAI CTGCAG 1 cut(s) 174
BspTNI GGTCTC 1 cut(s) 57
BsrFI RCCGGY 1 cut(s) 242
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 70
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 91
BstACI GRCGYC 1 cut(s) 159
BstC8I GCNNGC 3 cut(s) 201, 207, 244
BstDEI CTNAG 1 cut(s) 84
BstEII GGTNACC 1 cut(s) 53
BstFNI CGCG 2 cut(s) 51, 75
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 3 cut(s) 169, 199, 205
BstKTI GATC 1 cut(s) 73
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 1 cut(s) 70
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 3 cut(s) 191, 200, 209
BstNI CCWGG 1 cut(s) 91
BstPI GGTNACC 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 89
BstSFI CTRYAG 1 cut(s) 170
BstUI CGCG 2 cut(s) 51, 75
BstV1I GCAGC 2 cut(s) 124, 156
BstZI CGGCCG 1 cut(s) 210
BsuRI GGCC 2 cut(s) 89, 212
BtuMI TCGCGA 1 cut(s) 75
Cac8I GCNNGC 3 cut(s) 201, 207, 244
CfoI GCGC 3 cut(s) 169, 199, 205
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 1 cut(s) 93
CseI GACGC 1 cut(s) 167
CviJI RGCY 4 cut(s) 83, 89, 115, 212
CviKI_1 RGCY 4 cut(s) 83, 89, 115, 212
DdeI CTNAG 1 cut(s) 84
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 210
EagI CGGCCG 1 cut(s) 210
EclXI CGGCCG 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 85
Eco31I GGTCTC 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 93
Eco52I CGGCCG 1 cut(s) 210
Eco91I GGTNACC 1 cut(s) 53
EcoO65I GGTNACC 1 cut(s) 53
EcoRII CCWGG 1 cut(s) 89
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 162
FaqI GGGAC 1 cut(s) 170
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 5 cut(s) 113, 170, 195, 210, 247
FriOI GRGCYC 1 cut(s) 85
Fsp4HI GCNGC 5 cut(s) 113, 170, 195, 210, 247
GlaI GCGC 3 cut(s) 168, 198, 204
GluI GCNGC 5 cut(s) 113, 170, 195, 210, 247
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 2 cut(s) 89, 212
HapII CCGG 3 cut(s) 96, 213, 243
HgaI GACGC 1 cut(s) 167
HhaI GCGC 3 cut(s) 169, 199, 205
Hin1I GRCGYC 1 cut(s) 159
Hin6I GCGC 3 cut(s) 167, 197, 203
HinP1I GCGC 3 cut(s) 167, 197, 203
HinfI GANTC 1 cut(s) 234
HpaII CCGG 3 cut(s) 96, 213, 243
Hpy188III TCNNGA 1 cut(s) 74
HpyCH4V TGCA 1 cut(s) 172
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 200, 209
HpyF3I CTNAG 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 159
HspAI GCGC 3 cut(s) 167, 197, 203
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
Kzo9I GATC 1 cut(s) 70
Lsp1109I GCAGC 2 cut(s) 124, 156
MaeIII GTNAC 1 cut(s) 53
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 3 cut(s) 4, 27, 37
MnlI CCTC 3 cut(s) 175, 209, 224
MroNI GCCGGC 1 cut(s) 242
MseI TTAA 2 cut(s) 26, 131
MspI CCGG 3 cut(s) 96, 213, 243
MspR9I CCNGG 1 cut(s) 91
MvaI CCWGG 1 cut(s) 91
MvnI CGCG 2 cut(s) 51, 75
MwoI GCNNNNNNNGC 3 cut(s) 191, 200, 209
NaeI GCCGGC 1 cut(s) 244
NdeII GATC 1 cut(s) 70
NgoMIV GCCGGC 1 cut(s) 242
NmeAIII GCCGAG 1 cut(s) 173
NruI TCGCGA 1 cut(s) 75
PdiI GCCGGC 1 cut(s) 244
PfeI GAWTC 1 cut(s) 234
PkrI GCNGC 5 cut(s) 114, 171, 196, 211, 248
PshBI ATTAAT 1 cut(s) 26
Psp6I CCWGG 1 cut(s) 89
PspEI GGTNACC 1 cut(s) 53
PspGI CCWGG 1 cut(s) 89
PspPI GGNCC 1 cut(s) 93
PstI CTGCAG 1 cut(s) 174
RruI TCGCGA 1 cut(s) 75
SaqAI TTAA 2 cut(s) 26, 131
SatI GCNGC 5 cut(s) 113, 170, 195, 210, 247
Sau3AI GATC 1 cut(s) 70
Sau96I GGNCC 1 cut(s) 93
ScrFI CCNGG 1 cut(s) 91
SduI GDGCHC 1 cut(s) 85
SetI ASST 1 cut(s) 95
SfcI CTRYAG 1 cut(s) 170
SinI GGWCC 1 cut(s) 93
Sse9I AATT 3 cut(s) 4, 27, 37
SsiI CCGC 5 cut(s) 51, 194, 209, 227, 246
StyD4I CCNGG 1 cut(s) 89
StyI CCWWGG 1 cut(s) 162
TaqI TCGA 1 cut(s) 18
TaqII GACCGA 1 cut(s) 81
TasI AATT 3 cut(s) 4, 27, 37
TauI GCSGC 3 cut(s) 197, 212, 249
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 2 cut(s) 26, 131
Tru9I TTAA 2 cut(s) 26, 131
TseI GCWGC 2 cut(s) 112, 169
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 93
VspI ATTAAT 1 cut(s) 26
XapI RAATTY 2 cut(s) 4, 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.