Rroxscaffold_7G00184600

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002526
Physical Location & Seq
Reverse (-)
23922037 .. 23923521
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00184600.1

Sequence Viewer

Length: 240 bp
ATGCGACCTATTGTTTCCATAAACGATGACACTTTGCATTGCGAACGCGGTAACCAGAGAGACCGAGATCGTGAAAGGGCTCAGGCCAGGTCCGGCAACAAGACCAAGCAGGCCAAAACCGATGGATTAACCCCTGAACAACGCCGAGAAAGGGACGCCAAGGCGCTGCAGGAAAAGGCGGCAAAGAAGGCGGCGCAGGCGCAGGCGGCCGGAGGGATCAATGCTGGCGGCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

79

Amino Acids

8.67

Weight (kDa)

10.27

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SERF-like_N PF04419 16 - 49 1.9e-07 Small EDRK-rich factor 1/2-like, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 48
AciI CCGC 5 cut(s) 48, 179, 191, 206, 228
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 207
AcyI GRCGYC 1 cut(s) 156
AfiI CCNNNNNNNGG 1 cut(s) 151
AjnI CCWGG 1 cut(s) 86
Alw26I GTCTC 1 cut(s) 54
AlwI GGATC 1 cut(s) 224
AoxI GGCC 3 cut(s) 84, 111, 207
ApeKI GCWGC 1 cut(s) 166
AspLEI GCGC 3 cut(s) 166, 196, 202
AspS9I GGNCC 1 cut(s) 90
AvaII GGWCC 1 cut(s) 90
BanII GRGCYC 1 cut(s) 82
BbvI GCAGC 1 cut(s) 153
BccI CCATC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 167
BfoI RGCGCY 1 cut(s) 167
BisI GCNGC 5 cut(s) 167, 180, 192, 207, 229
BlsI GCNGC 5 cut(s) 168, 181, 193, 208, 230
Bme1390I CCNGG 1 cut(s) 88
Bme18I GGWCC 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 88
Bpu10I CCTNAGC 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 159
Bsc4I CCNNNNNNNGG 1 cut(s) 151
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 151
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 95
BseX3I CGGCCG 1 cut(s) 207
BseXI GCAGC 1 cut(s) 153
Bsh1236I CGCG 1 cut(s) 48
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 3 cut(s) 86, 113, 209
BsiEI CGRYCG 1 cut(s) 210
BsiSI CCGG 2 cut(s) 93, 210
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 151
BsmAI GTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 3 cut(s) 86, 113, 209
Bso31I GGTCTC 1 cut(s) 54
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 2 cut(s) 67, 216
BspACI CCGC 5 cut(s) 48, 179, 191, 206, 228
BspANI GGCC 3 cut(s) 86, 113, 209
BspCNI CTCAG 1 cut(s) 94
BspFNI CGCG 1 cut(s) 48
BspMAI CTGCAG 1 cut(s) 171
BspPI GGATC 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 2 cut(s) 67, 216
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 88
BstACI GRCGYC 1 cut(s) 156
BstC8I GCNNGC 4 cut(s) 111, 198, 204, 226
BstDEI CTNAG 1 cut(s) 81
BstEII GGTNACC 1 cut(s) 50
BstFNI CGCG 1 cut(s) 48
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 3 cut(s) 166, 196, 202
BstKTI GATC 2 cut(s) 70, 219
BstMAI GTCTC 1 cut(s) 54
BstMBI GATC 2 cut(s) 67, 216
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 3 cut(s) 188, 197, 206
BstNI CCWGG 1 cut(s) 88
BstPI GGTNACC 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 86
BstSFI CTRYAG 1 cut(s) 167
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 153
BstZI CGGCCG 1 cut(s) 207
BsuRI GGCC 3 cut(s) 86, 113, 209
Cac8I GCNNGC 4 cut(s) 111, 198, 204, 226
CfoI GCGC 3 cut(s) 166, 196, 202
Cfr13I GGNCC 1 cut(s) 90
CseI GACGC 1 cut(s) 164
CviJI RGCY 4 cut(s) 80, 86, 113, 209
CviKI_1 RGCY 4 cut(s) 80, 86, 113, 209
DdeI CTNAG 1 cut(s) 81
DpnI GATC 2 cut(s) 69, 218
DpnII GATC 2 cut(s) 67, 216
EaeI YGGCCR 1 cut(s) 207
EagI CGGCCG 1 cut(s) 207
EclXI CGGCCG 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 90
Eco52I CGGCCG 1 cut(s) 207
Eco91I GGTNACC 1 cut(s) 50
EcoO65I GGTNACC 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 159
FaiI YATR 1 cut(s) 20
FaqI GGGAC 1 cut(s) 167
Fnu4HI GCNGC 5 cut(s) 167, 180, 192, 207, 229
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 5 cut(s) 167, 180, 192, 207, 229
GlaI GCGC 3 cut(s) 165, 195, 201
GluI GCNGC 5 cut(s) 167, 180, 192, 207, 229
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 3 cut(s) 86, 113, 209
HapII CCGG 2 cut(s) 93, 210
HgaI GACGC 1 cut(s) 164
HhaI GCGC 3 cut(s) 166, 196, 202
Hin1I GRCGYC 1 cut(s) 156
Hin6I GCGC 3 cut(s) 164, 194, 200
HinP1I GCGC 3 cut(s) 164, 194, 200
HpaII CCGG 2 cut(s) 93, 210
Hpy188III TCNNGA 1 cut(s) 71
HpyAV CCTTC 1 cut(s) 181
HpyCH4V TGCA 2 cut(s) 37, 169
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 197, 206
HpyF3I CTNAG 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 156
HspAI GCGC 3 cut(s) 164, 194, 200
Kzo9I GATC 2 cut(s) 67, 216
Lsp1109I GCAGC 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 50
MalI GATC 2 cut(s) 69, 218
MboI GATC 2 cut(s) 67, 216
MhlI GDGCHC 1 cut(s) 82
MnlI CCTC 1 cut(s) 206
MseI TTAA 1 cut(s) 128
MspI CCGG 2 cut(s) 93, 210
MspR9I CCNGG 1 cut(s) 88
MvaI CCWGG 1 cut(s) 88
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 3 cut(s) 188, 197, 206
NdeII GATC 2 cut(s) 67, 216
NmeAIII GCCGAG 1 cut(s) 170
PkrI GCNGC 5 cut(s) 168, 181, 193, 208, 230
Psp6I CCWGG 1 cut(s) 86
PspEI GGTNACC 1 cut(s) 50
PspGI CCWGG 1 cut(s) 86
PspPI GGNCC 1 cut(s) 90
PstI CTGCAG 1 cut(s) 171
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 5 cut(s) 167, 180, 192, 207, 229
Sau3AI GATC 2 cut(s) 67, 216
Sau96I GGNCC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 88
SduI GDGCHC 1 cut(s) 82
SetI ASST 2 cut(s) 10, 92
SfcI CTRYAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 90
SsiI CCGC 5 cut(s) 48, 179, 191, 206, 228
StyD4I CCNGG 1 cut(s) 86
StyI CCWWGG 1 cut(s) 159
TaqII GACCGA 1 cut(s) 78
TauI GCSGC 4 cut(s) 182, 194, 209, 231
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TseI GCWGC 1 cut(s) 166
VpaK11BI GGWCC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.