Prupe.1G008500_v2.0.a1

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0002526
Physical Location & Seq
Reverse (-)
700654 .. 701484
831 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G008500.1

Sequence Viewer

Length: 195 bp
ATGACTCGCGGCAACCAGAGAGAGAAAGATCGGGAAAGAGCTCAGGCTCGGGGCGGCAAGGGTAAGAACAAGGACGATGGCTTGACCCCAGAACAGCGCCGAGAGAGGGATGCGAAAGCGCTCCAAGAAAAGCAGGCGAAGAAGGCCGCGGGGGCAGCTCCGGGAGGGAATTCTTCCGGAGGTAAAAGCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

65

Amino Acids

6.86

Weight (kDa)

10.53

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 9, 149
AccIII TCCGGA 1 cut(s) 176
AciI CCGC 4 cut(s) 9, 54, 147, 149
AcsI RAATTY 1 cut(s) 169
AfeI AGCGCT 1 cut(s) 120
AfiI CCNNNNNNNGG 1 cut(s) 106
AluBI AGCT 2 cut(s) 41, 158
AluI AGCT 2 cut(s) 41, 158
Alw21I GWGCWC 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 48
Aor13HI TCCGGA 1 cut(s) 176
Aor51HI AGCGCT 1 cut(s) 120
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 1 cut(s) 169
AspLEI GCGC 2 cut(s) 99, 121
AsuC2I CCSGG 1 cut(s) 162
AvaI CYCGRG 1 cut(s) 48
BanII GRGCYC 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 1 cut(s) 167
BccI CCATC 1 cut(s) 71
BcnI CCSGG 1 cut(s) 162
BfoI RGCGCY 2 cut(s) 100, 122
BglI GCCNNNNNGGC 1 cut(s) 152
BisI GCNGC 4 cut(s) 10, 55, 147, 156
BlsI GCNGC 4 cut(s) 11, 56, 148, 157
Bme1390I CCNGG 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 1 cut(s) 100
Bpu10I CCTNAGC 1 cut(s) 42
BpuMI CCSGG 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 1 cut(s) 106
BseAI TCCGGA 1 cut(s) 176
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 106
BseMII CTCAG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 167
Bsh1236I CGCG 2 cut(s) 9, 149
BshFI GGCC 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 43
BsiHKCI CYCGRG 1 cut(s) 48
BsiSI CCGG 2 cut(s) 161, 177
BslI CCNNNNNNNGG 1 cut(s) 106
BsnI GGCC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 43
Bsp13I TCCGGA 1 cut(s) 176
Bsp143I GATC 1 cut(s) 28
BspACI CCGC 4 cut(s) 9, 54, 147, 149
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 1 cut(s) 55
BspEI TCCGGA 1 cut(s) 176
BspFNI CGCG 2 cut(s) 9, 149
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 1 cut(s) 42
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 115
BstFNI CGCG 2 cut(s) 9, 149
BstH2I RGCGCY 2 cut(s) 100, 122
BstHHI GCGC 2 cut(s) 99, 121
BstKTI GATC 1 cut(s) 31
BstMBI GATC 1 cut(s) 28
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 155
BstSCI CCNGG 1 cut(s) 160
BstUI CGCG 2 cut(s) 9, 149
BstV1I GCAGC 1 cut(s) 167
BsuRI GGCC 1 cut(s) 146
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 1 cut(s) 115
Cac8I GCNNGC 1 cut(s) 135
CfoI GCGC 2 cut(s) 99, 121
Cfr42I CCGCGG 1 cut(s) 150
CviJI RGCY 5 cut(s) 41, 47, 81, 146, 158
CviKI_1 RGCY 5 cut(s) 41, 47, 81, 146, 158
DdeI CTNAG 1 cut(s) 42
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 41
Eco24I GRGCYC 1 cut(s) 43
Eco47III AGCGCT 1 cut(s) 120
Eco53kI GAGCTC 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 41
EcoRI GAATTC 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 43
FauI CCCGC 1 cut(s) 142
Fnu4HI GCNGC 4 cut(s) 10, 55, 147, 156
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 4 cut(s) 10, 55, 147, 156
GlaI GCGC 2 cut(s) 98, 120
GluI GCNGC 4 cut(s) 10, 55, 147, 156
HaeII RGCGCY 2 cut(s) 100, 122
HaeIII GGCC 1 cut(s) 146
HapII CCGG 2 cut(s) 161, 177
HhaI GCGC 2 cut(s) 99, 121
Hin6I GCGC 2 cut(s) 97, 119
HinP1I GCGC 2 cut(s) 97, 119
HinfI GANTC 1 cut(s) 4
HpaII CCGG 2 cut(s) 161, 177
Hpy188III TCNNGA 2 cut(s) 32, 177
HpyAV CCTTC 1 cut(s) 136
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 155
HpyF3I CTNAG 1 cut(s) 42
HspAI GCGC 2 cut(s) 97, 119
Kpn2I TCCGGA 1 cut(s) 176
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 1 cut(s) 28
LmnI GCTCC 2 cut(s) 126, 163
LpnPI CCDG 6 cut(s) 29, 29, 102, 119, 174, 190
Lsp1109I GCAGC 1 cut(s) 167
LweI GCATC 1 cut(s) 100
MalI GATC 1 cut(s) 30
MboI GATC 1 cut(s) 28
MboII GAAGA 2 cut(s) 151, 165
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 1 cut(s) 169
MnlI CCTC 3 cut(s) 99, 158, 173
MroI TCCGGA 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 2 cut(s) 161, 177
MspR9I CCNGG 1 cut(s) 162
MvnI CGCG 2 cut(s) 9, 149
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 155
NciI CCSGG 1 cut(s) 162
NdeII GATC 1 cut(s) 28
NmeAIII GCCGAG 1 cut(s) 125
PfoI TCCNGGA 1 cut(s) 160
PkrI GCNGC 4 cut(s) 11, 56, 148, 157
Psp124BI GAGCTC 1 cut(s) 43
SacI GAGCTC 1 cut(s) 43
SacII CCGCGG 1 cut(s) 150
SatI GCNGC 4 cut(s) 10, 55, 147, 156
Sau3AI GATC 1 cut(s) 28
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 1 cut(s) 43
SetI ASST 3 cut(s) 43, 160, 184
SfaNI GCATC 1 cut(s) 100
Sfr303I CCGCGG 1 cut(s) 150
SgrBI CCGCGG 1 cut(s) 150
Sse9I AATT 1 cut(s) 169
SsiI CCGC 4 cut(s) 9, 54, 147, 149
SstI GAGCTC 1 cut(s) 43
StyD4I CCNGG 1 cut(s) 160
TasI AATT 1 cut(s) 169
TauI GCSGC 3 cut(s) 12, 57, 149
TseI GCWGC 1 cut(s) 155
XapI RAATTY 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.