RLG00000012737

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002526
Physical Location & Seq
Reverse (-)
21864162 .. 21865180
1019 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012737

Sequence Viewer

Length: 333 bp
ATGGGTTCTCAACAACTAGTCCTTTATATACCCACCCGCCTCTTCTCGCTCGGACACATTCAACCTTCTCTGATTTCCTCCTCCGTCTTACACCCATCCGCGCTCACATCGTCAATCAGCGAACGCGGTAACCAGAGAGACCGAGATCGTGAAAGGGCTCAGGCCAGGTCCGGCAACAAGACCAAGCAGCCCAAAACCGATGGATTAACCCCGGAACAACGCCGAGAAAGGGACGCCAAGGCGCTGCAGGAAAAGGCGGCAAAGAAGGCGGCGCAGGCGCAGGCGGCCGGAGGGAACAAAGCTGGAGGGATCAATGCTGGCGGCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

111

Amino Acids

11.78

Weight (kDa)

10.78

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SERF-like_N PF04419 42 - 75 6e-08 Small EDRK-rich factor 1/2-like, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 101, 126
AciI CCGC 7 cut(s) 37, 99, 126, 257, 269, 284, 321
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 234
AfiI CCNNNNNNNGG 1 cut(s) 229
AgsI TTSAA 1 cut(s) 62
AhlI ACTAGT 1 cut(s) 16
AjnI CCWGG 1 cut(s) 164
AluBI AGCT 1 cut(s) 302
AluI AGCT 1 cut(s) 302
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 1 cut(s) 317
AoxI GGCC 2 cut(s) 162, 285
ApeKI GCWGC 2 cut(s) 187, 244
AspLEI GCGC 4 cut(s) 103, 244, 274, 280
AspS9I GGNCC 1 cut(s) 168
AsuC2I CCSGG 1 cut(s) 212
AvaII GGWCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 160
BbvI GCAGC 2 cut(s) 199, 231
BccI CCATC 2 cut(s) 103, 194
BciT130I CCWGG 1 cut(s) 166
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 1 cut(s) 132
BcuI ACTAGT 1 cut(s) 16
BfaI CTAG 1 cut(s) 17
BfmI CTRYAG 1 cut(s) 245
BfoI RGCGCY 1 cut(s) 245
BisI GCNGC 6 cut(s) 188, 245, 258, 270, 285, 322
BlsI GCNGC 6 cut(s) 189, 246, 259, 271, 286, 323
Bme1390I CCNGG 2 cut(s) 166, 212
Bme18I GGWCC 1 cut(s) 168
BmgT120I GGNCC 1 cut(s) 168
BmrFI CCNGG 2 cut(s) 166, 212
BpmI CTGGAG 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 159
BpuMI CCSGG 1 cut(s) 212
BsaHI GRCGYC 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 210, 237
Bsc4I CCNNNNNNNGG 1 cut(s) 229
BseBI CCWGG 1 cut(s) 166
BseDI CCNNGG 2 cut(s) 210, 237
BseGI GGATG 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 173
BseRI GAGGAG 1 cut(s) 70
BseX3I CGGCCG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 199, 231
Bsh1236I CGCG 2 cut(s) 101, 126
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 2 cut(s) 164, 287
BsiEI CGRYCG 1 cut(s) 288
BsiSI CCGG 3 cut(s) 171, 212, 288
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 2 cut(s) 164, 287
Bso31I GGTCTC 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 2 cut(s) 145, 309
BspACI CCGC 7 cut(s) 37, 99, 126, 257, 269, 284, 321
BspANI GGCC 2 cut(s) 164, 287
BspCNI CTCAG 1 cut(s) 172
BspFNI CGCG 2 cut(s) 101, 126
BspMAI CTGCAG 1 cut(s) 249
BspPI GGATC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 132
BssECI CCNNGG 2 cut(s) 210, 237
BssMI GATC 2 cut(s) 145, 309
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 237
Bst2UI CCWGG 1 cut(s) 166
Bst6I CTCTTC 1 cut(s) 47
BstACI GRCGYC 1 cut(s) 234
BstC8I GCNNGC 3 cut(s) 276, 282, 319
BstDEI CTNAG 1 cut(s) 159
BstEII GGTNACC 1 cut(s) 128
BstF5I GGATG 1 cut(s) 95
BstFNI CGCG 2 cut(s) 101, 126
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 4 cut(s) 103, 244, 274, 280
BstKTI GATC 2 cut(s) 148, 312
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 145, 309
BstMCI CGRYCG 1 cut(s) 288
BstMWI GCNNNNNNNGC 3 cut(s) 266, 275, 284
BstNI CCWGG 1 cut(s) 166
BstPI GGTNACC 1 cut(s) 128
BstSCI CCNGG 2 cut(s) 164, 210
BstSFI CTRYAG 1 cut(s) 245
BstUI CGCG 2 cut(s) 101, 126
BstV1I GCAGC 2 cut(s) 199, 231
BstZI CGGCCG 1 cut(s) 285
BsuRI GGCC 2 cut(s) 164, 287
BtsCI GGATG 1 cut(s) 95
Cac8I GCNNGC 3 cut(s) 276, 282, 319
CfoI GCGC 4 cut(s) 103, 244, 274, 280
Cfr13I GGNCC 1 cut(s) 168
CseI GACGC 1 cut(s) 242
CviJI RGCY 5 cut(s) 158, 164, 190, 287, 302
CviKI_1 RGCY 5 cut(s) 158, 164, 190, 287, 302
DdeI CTNAG 1 cut(s) 159
DpnI GATC 2 cut(s) 147, 311
DpnII GATC 2 cut(s) 145, 309
EaeI YGGCCR 1 cut(s) 285
EagI CGGCCG 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
EclXI CGGCCG 1 cut(s) 285
Eco130I CCWWGG 1 cut(s) 237
Eco24I GRGCYC 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 168
Eco52I CGGCCG 1 cut(s) 285
Eco91I GGTNACC 1 cut(s) 128
EcoO65I GGTNACC 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 164
EcoT14I CCWWGG 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 237
FaiI YATR 2 cut(s) 27, 29
FaqI GGGAC 1 cut(s) 245
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 6 cut(s) 188, 245, 258, 270, 285, 322
FokI GGATG 1 cut(s) 82
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 6 cut(s) 188, 245, 258, 270, 285, 322
FspBI CTAG 1 cut(s) 17
GlaI GCGC 4 cut(s) 102, 243, 273, 279
GluI GCNGC 6 cut(s) 188, 245, 258, 270, 285, 322
GsuI CTGGAG 1 cut(s) 324
HaeII RGCGCY 1 cut(s) 245
HaeIII GGCC 2 cut(s) 164, 287
HapII CCGG 3 cut(s) 171, 212, 288
HgaI GACGC 1 cut(s) 242
HhaI GCGC 4 cut(s) 103, 244, 274, 280
Hin1I GRCGYC 1 cut(s) 234
Hin6I GCGC 4 cut(s) 101, 242, 272, 278
HinP1I GCGC 4 cut(s) 101, 242, 272, 278
HpaII CCGG 3 cut(s) 171, 212, 288
Hpy188I TCNGA 2 cut(s) 53, 72
Hpy188III TCNNGA 1 cut(s) 149
HpyAV CCTTC 2 cut(s) 75, 259
HpyCH4V TGCA 1 cut(s) 247
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 275, 284
HpyF3I CTNAG 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 234
HspAI GCGC 4 cut(s) 101, 242, 272, 278
Kzo9I GATC 2 cut(s) 145, 309
Lsp1109I GCAGC 2 cut(s) 199, 231
MaeI CTAG 1 cut(s) 17
MaeIII GTNAC 1 cut(s) 128
MalI GATC 2 cut(s) 147, 311
MboI GATC 2 cut(s) 145, 309
MboII GAAGA 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 160
MnlI CCTC 5 cut(s) 50, 88, 91, 284, 299
MseI TTAA 1 cut(s) 206
MspI CCGG 3 cut(s) 171, 212, 288
MspR9I CCNGG 2 cut(s) 166, 212
MvaI CCWGG 1 cut(s) 166
MvnI CGCG 2 cut(s) 101, 126
MwoI GCNNNNNNNGC 3 cut(s) 266, 275, 284
NciI CCSGG 1 cut(s) 212
NdeII GATC 2 cut(s) 145, 309
NmeAIII GCCGAG 1 cut(s) 248
PkrI GCNGC 6 cut(s) 189, 246, 259, 271, 286, 323
Psp6I CCWGG 1 cut(s) 164
PspEI GGTNACC 1 cut(s) 128
PspGI CCWGG 1 cut(s) 164
PspPI GGNCC 1 cut(s) 168
PstI CTGCAG 1 cut(s) 249
SaqAI TTAA 1 cut(s) 206
SatI GCNGC 6 cut(s) 188, 245, 258, 270, 285, 322
Sau3AI GATC 2 cut(s) 145, 309
Sau96I GGNCC 1 cut(s) 168
ScrFI CCNGG 2 cut(s) 166, 212
SduI GDGCHC 1 cut(s) 160
SetI ASST 3 cut(s) 67, 170, 304
SfcI CTRYAG 1 cut(s) 245
SinI GGWCC 1 cut(s) 168
SpeI ACTAGT 1 cut(s) 16
SsiI CCGC 7 cut(s) 37, 99, 126, 257, 269, 284, 321
SspMI CTAG 1 cut(s) 17
StyD4I CCNGG 2 cut(s) 164, 210
StyI CCWWGG 1 cut(s) 237
TaqII GACCGA 1 cut(s) 156
TauI GCSGC 4 cut(s) 260, 272, 287, 324
Tru1I TTAA 1 cut(s) 206
Tru9I TTAA 1 cut(s) 206
TseI GCWGC 2 cut(s) 187, 244
TspGWI ACGGA 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 168
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.