Rh4BG191300

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002526
Physical Location & Seq
Forward (+)
33111753 .. 33115867
4115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG191300.1

Sequence Viewer

Length: 558 bp
ATGGACCCGAATCGGGTCGAGGGCAGCAACCGGGTCGTCCAGCTGCGTCGGTCTTCCGGAGCCGGGTTGCCGGTGCAGACGAGTGAAGTGACGTCAGAATCCGAAGAGGAATCCGGTCCGAGATTGGTGAGTCTCAAGGGCAGCACCTCGGAGGCGTGCGTGGAAGGGGCAGTAGCGCGGCCGGTCTTCTGGGTCGGGCTGTGCGCTGCGGTTGAGGCCGGAAGAAGGATTTGGGCGATGCAGAGAGGTTGCTTAGGCGATGTGTGGCTGGAAGAAAGGCCTGGACGAGGGGTCTGGCTGGGCAACGTGCTGGCACGGTTTGCAGGCTGGCTGAGGCAAGGAGCCACACTGAGTACAAATTCTGTTCAAAGAGAGAAGGATCGGGAGAGAGCTCAGGCTCGGGGCGGCAAGGGTAGCAAGAACAAAGACGATGGCTTGACCCCAGAACAGCGCCGAGAGAGGGATGCCAAAGCACTCCAAGAAAAGCTGGCCAAGAAGGCTGATAAGGGTGCAGGGGCTGCCGGAGCTAACTCCTCCTCTGGAGGTAAAAGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

185

Amino Acids

19.77

Weight (kDa)

9.88

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 95
AccII CGCG 1 cut(s) 178
AccIII TCCGGA 1 cut(s) 56
AciI CCGC 3 cut(s) 178, 209, 405
AclWI GGATC 1 cut(s) 387
AcoI YGGCCR 2 cut(s) 179, 489
AcsI RAATTY 1 cut(s) 358
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 5 cut(s) 13, 63, 225, 287, 460
AgsI TTSAA 1 cut(s) 368
AhdI GACNNNNNGTC 1 cut(s) 290
AjnI CCWGG 1 cut(s) 280
AjuI GAANNNNNNNTTGG 2 cut(s) 214, 246
AluBI AGCT 4 cut(s) 43, 392, 487, 527
AluI AGCT 4 cut(s) 43, 392, 487, 527
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 1 cut(s) 387
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 399
Aor13HI TCCGGA 1 cut(s) 56
AoxI GGCC 4 cut(s) 179, 216, 278, 489
ApeKI GCWGC 5 cut(s) 24, 43, 141, 206, 518
ApoI RAATTY 1 cut(s) 358
AspLEI GCGC 3 cut(s) 178, 206, 453
AspS9I GGNCC 2 cut(s) 4, 116
AsuC2I CCSGG 2 cut(s) 32, 64
AsuHPI GGTGA 1 cut(s) 139
AvaI CYCGRG 1 cut(s) 399
AvaII GGWCC 2 cut(s) 4, 116
BalI TGGCCA 1 cut(s) 491
BanII GRGCYC 1 cut(s) 394
BbsI GAAGAC 2 cut(s) 45, 178
Bbv12I GWGCWC 1 cut(s) 394
BbvCI CCTCAGC 1 cut(s) 332
BbvI GCAGC 5 cut(s) 30, 36, 153, 193, 505
BccI CCATC 1 cut(s) 425
BcgI CGANNNNNNTGC 2 cut(s) 16, 50
BciT130I CCWGG 1 cut(s) 282
BcnI CCSGG 2 cut(s) 32, 64
BcoDI GTCTC 1 cut(s) 137
BfoI RGCGCY 1 cut(s) 454
BglI GCCNNNNNGGC 1 cut(s) 497
BisI GCNGC 7 cut(s) 25, 44, 142, 179, 207, 406, 519
BlsI GCNGC 7 cut(s) 26, 45, 143, 180, 208, 407, 520
Bme1390I CCNGG 3 cut(s) 32, 64, 282
Bme18I GGWCC 2 cut(s) 4, 116
BmeRI GACNNNNNGTC 1 cut(s) 290
BmeT110I CYCGRG 1 cut(s) 399
BmgT120I GGNCC 2 cut(s) 4, 116
BmiI GGNNCC 3 cut(s) 6, 61, 343
BmrFI CCNGG 3 cut(s) 32, 64, 282
BmsI GCATC 2 cut(s) 228, 454
BpiI GAAGAC 2 cut(s) 45, 178
Bpu10I CCTNAGC 3 cut(s) 253, 332, 393
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 2 cut(s) 32, 64
BsaHI GRCGYC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 2 cut(s) 56, 113
Bsc4I CCNNNNNNNGG 5 cut(s) 13, 63, 225, 287, 460
Bse118I RCCGGY 2 cut(s) 70, 181
BseAI TCCGGA 1 cut(s) 56
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 469
BseLI CCNNNNNNNGG 5 cut(s) 13, 63, 225, 287, 460
BseMII CTCAG 3 cut(s) 323, 341, 407
BseRI GAGGAG 2 cut(s) 523, 526
BseX3I CGGCCG 1 cut(s) 179
BseXI GCAGC 5 cut(s) 30, 36, 153, 193, 505
BseYI CCCAGC 1 cut(s) 298
BsgI GTGCAG 2 cut(s) 95, 531
Bsh1236I CGCG 1 cut(s) 178
Bsh1285I CGRYCG 1 cut(s) 182
BshFI GGCC 4 cut(s) 181, 218, 280, 491
BsiEI CGRYCG 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 394
BsiHKCI CYCGRG 1 cut(s) 399
BsiSI CCGG 8 cut(s) 31, 57, 63, 71, 114, 182, 219, 522
BslI CCNNNNNNNGG 5 cut(s) 13, 63, 225, 287, 460
BsmAI GTCTC 1 cut(s) 137
BsnI GGCC 4 cut(s) 181, 218, 280, 491
BsoBI CYCGRG 1 cut(s) 399
Bsp1286I GDGCHC 1 cut(s) 394
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 1 cut(s) 379
BspACI CCGC 3 cut(s) 178, 209, 405
BspANI GGCC 4 cut(s) 181, 218, 280, 491
BspCNI CTCAG 3 cut(s) 324, 342, 406
BspEI TCCGGA 1 cut(s) 56
BspFNI CGCG 1 cut(s) 178
BspLI GGNNCC 3 cut(s) 6, 61, 343
BspPI GGATC 1 cut(s) 387
BsrFI RCCGGY 2 cut(s) 70, 181
BssAI RCCGGY 2 cut(s) 70, 181
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 1 cut(s) 379
BssNI GRCGYC 1 cut(s) 92
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 1 cut(s) 318
Bst6I CTCTTC 1 cut(s) 99
BstACI GRCGYC 1 cut(s) 92
BstAPI GCANNNNNTGC 2 cut(s) 320, 518
BstC8I GCNNGC 5 cut(s) 157, 312, 325, 329, 489
BstDEI CTNAG 4 cut(s) 253, 332, 350, 393
BstENI CCTNNNNNAGG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 1 cut(s) 178
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 3 cut(s) 178, 206, 453
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 1 cut(s) 379
BstMCI CGRYCG 1 cut(s) 182
BstMWI GCNNNNNNNGC 6 cut(s) 215, 320, 414, 497, 518, 524
BstNI CCWGG 1 cut(s) 282
BstSCI CCNGG 3 cut(s) 30, 62, 280
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 5 cut(s) 30, 36, 153, 193, 505
BstV2I GAAGAC 2 cut(s) 45, 178
BstZI CGGCCG 1 cut(s) 179
BsuRI GGCC 4 cut(s) 181, 218, 280, 491
BtgZI GCGATG 2 cut(s) 251, 273
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 1 cut(s) 347
Cac8I GCNNGC 5 cut(s) 157, 312, 325, 329, 489
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 3 cut(s) 178, 206, 453
Cfr10I RCCGGY 2 cut(s) 70, 181
Cfr13I GGNCC 2 cut(s) 4, 116
CpoI CGGWCCG 1 cut(s) 116
CseI GACGC 1 cut(s) 35
Csp6I GTAC 1 cut(s) 354
CspI CGGWCCG 1 cut(s) 116
CviQI GTAC 1 cut(s) 354
DdeI CTNAG 4 cut(s) 253, 332, 350, 393
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
DriI GACNNNNNGTC 1 cut(s) 290
EaeI YGGCCR 2 cut(s) 179, 489
EagI CGGCCG 1 cut(s) 179
Eam1104I CTCTTC 1 cut(s) 99
Eam1105I GACNNNNNGTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 392
EclXI CGGCCG 1 cut(s) 179
Eco147I AGGCCT 1 cut(s) 280
Eco24I GRGCYC 1 cut(s) 394
Eco47I GGWCC 2 cut(s) 4, 116
Eco52I CGGCCG 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 392
EcoNI CCTNNNNNAGG 1 cut(s) 285
EcoRII CCWGG 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 394
Fnu4HI GCNGC 7 cut(s) 25, 44, 142, 179, 207, 406, 519
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 7 cut(s) 25, 44, 142, 179, 207, 406, 519
GlaI GCGC 3 cut(s) 177, 205, 452
GluI GCNGC 7 cut(s) 25, 44, 142, 179, 207, 406, 519
GsaI CCCAGC 1 cut(s) 302
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 4 cut(s) 181, 218, 280, 491
HapII CCGG 8 cut(s) 31, 57, 63, 71, 114, 182, 219, 522
HgaI GACGC 1 cut(s) 35
HhaI GCGC 3 cut(s) 178, 206, 453
Hin1I GRCGYC 1 cut(s) 92
Hin6I GCGC 3 cut(s) 176, 204, 451
HinP1I GCGC 3 cut(s) 176, 204, 451
HinfI GANTC 4 cut(s) 10, 98, 110, 130
HpaII CCGG 8 cut(s) 31, 57, 63, 71, 114, 182, 219, 522
HphI GGTGA 1 cut(s) 139
Hpy188I TCNGA 4 cut(s) 97, 103, 120, 151
Hpy188III TCNNGA 3 cut(s) 57, 383, 540
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 4 cut(s) 158, 219, 370, 490
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 2 cut(s) 92, 306
HpyCH4V TGCA 4 cut(s) 76, 241, 323, 512
HpyF10VI GCNNNNNNNGC 6 cut(s) 215, 320, 414, 497, 518, 524
HpyF3I CTNAG 4 cut(s) 253, 332, 350, 393
HpySE526I ACGT 2 cut(s) 92, 306
Hsp92I GRCGYC 1 cut(s) 92
HspAI GCGC 3 cut(s) 176, 204, 451
Kpn2I TCCGGA 1 cut(s) 56
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 3 cut(s) 59, 341, 524
Lsp1109I GCAGC 5 cut(s) 30, 36, 153, 193, 505
LweI GCATC 2 cut(s) 228, 454
MaeII ACGT 2 cut(s) 92, 306
MaeIII GTNAC 1 cut(s) 88
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MboII GAAGA 5 cut(s) 45, 116, 178, 234, 284
MhlI GDGCHC 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 1 cut(s) 358
MluNI TGGCCA 1 cut(s) 491
MlyI GAGTC 1 cut(s) 139
Mox20I TGGCCA 1 cut(s) 491
MroI TCCGGA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 491
Msp20I TGGCCA 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 43
MspI CCGG 8 cut(s) 31, 57, 63, 71, 114, 182, 219, 522
MspR9I CCNGG 3 cut(s) 32, 64, 282
MvaI CCWGG 1 cut(s) 282
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 6 cut(s) 215, 320, 414, 497, 518, 524
NciI CCSGG 2 cut(s) 32, 64
NdeII GATC 1 cut(s) 379
NlaIV GGNNCC 3 cut(s) 6, 61, 343
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 1 cut(s) 88
PceI AGGCCT 1 cut(s) 280
PfeI GAWTC 3 cut(s) 10, 98, 110
PkrI GCNGC 7 cut(s) 26, 45, 143, 180, 208, 407, 520
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
Psp124BI GAGCTC 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 280
PspFI CCCAGC 1 cut(s) 298
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 3 cut(s) 6, 61, 343
PspPI GGNCC 2 cut(s) 4, 116
PstNI CAGNNNCTG 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 43
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
Rsr2I CGGWCCG 1 cut(s) 116
RsrII CGGWCCG 1 cut(s) 116
SacI GAGCTC 1 cut(s) 394
SatI GCNGC 7 cut(s) 25, 44, 142, 179, 207, 406, 519
Sau3AI GATC 1 cut(s) 379
Sau96I GGNCC 2 cut(s) 4, 116
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 3 cut(s) 32, 64, 282
SduI GDGCHC 1 cut(s) 394
SetI ASST 9 cut(s) 45, 95, 149, 250, 309, 394, 489, 529, 547
SfaNI GCATC 2 cut(s) 228, 454
SinI GGWCC 2 cut(s) 4, 116
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 1 cut(s) 358
SseBI AGGCCT 1 cut(s) 280
SsiI CCGC 3 cut(s) 178, 209, 405
SstI GAGCTC 1 cut(s) 394
StuI AGGCCT 1 cut(s) 280
StyD4I CCNGG 3 cut(s) 30, 62, 280
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 2 cut(s) 95, 309
TaqI TCGA 1 cut(s) 18
TaqII GACCGA 1 cut(s) 39
TasI AATT 1 cut(s) 358
TatI WGTACW 1 cut(s) 353
TauI GCSGC 2 cut(s) 181, 408
TfiI GAWTC 3 cut(s) 10, 98, 110
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 5 cut(s) 24, 43, 141, 206, 518
Tsp45I GTSAC 1 cut(s) 88
TspRI CASTG 1 cut(s) 354
VpaK11BI GGWCC 2 cut(s) 4, 116
XagI CCTNNNNNAGG 1 cut(s) 285
XapI RAATTY 1 cut(s) 358
ZraI GACGTC 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.