FvH4_5g09421

Lysosomal alpha-mannosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001301
Physical Location & Seq
Reverse (-)
5464384 .. 5470630
6247 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g09421.t1

Sequence Viewer

Length: 3033 bp
ATGGCAGTTGTGGCTTACTTTCCGGTTGTATTAGTATTTACTGTGTTTCTGTACGCGGCGGAGGCCAAGTACATACAGTACAATACGACGTCGGTTCTTGTCCCCGGGAAGCTGAACGTTCATTTGGTGCCTCACACCCACGACGACGTTGGATGGTTGAAGACCGTTGACCAGTACTACGTTGGCTCCAATAATTCCATCCAGGGAGCTTGTGTTCAGAACGTGCTGGATTCGCTGATTCCGGCATTGCTGGCTGATAAGAACCGCAAGTTCGTATACGTCGAGCAGGCGTTTTTCCAAAGATGGTGGAGAGATCAGAGCGAGGAGGTGCAAGACACGGTGAAGGAGCTGGTCAGCTCCGGTCAACTTGAGCTCATAAATGGGGGGATGTGTATGCACGATGAGGCGGCTCCCCATTACATTGATATGATTGATCAGACGACTCTGGGGCATCGGTTTATTAAACAGGAGTTCAATGTGACTCCAAGAATTGGCTGGCAGATTGATCCGTTTGGACATTCTGCTGTGCAGGCTTACCTTTTGGGGGCTGAGGTTGGATTTGACTCTCTTTTCTTTGGGAGAATTGATTACCAAGACAGAGCTAAACGAAAAGTTGACAAAAGCCTTGAGGTTGTGTGGCGGGGTTCTAAGAGTCTAGGTTCATCTGCGCAGATTTTTGCTGGTGCATTTCCTGCAAACTATGAACCTCCTAGTGATAATTTCTACTTTGAAGTTAATGATGCTTCCCCTATTGTACAAGATGATATGGATTTGTTTGACTACAATGTGCCTGACCGTGTTAATGATTTCGTATCTGCTGCAATGTTACAAGCGAACATTACTCGGACAAATCATATCATGTGGACCATGGGAACAGATTTCAAGTATCAATATGCTCATACTTGGTTTCGGCAGATGGATAAATTCATTAACTATGTCAATCAAGATGGGCGTGTCAATGCCCTGTACTCAACCCCATCAATATATACTGATGCCAAATACGCAGCAGATGAGTCATGGCCAATCAAGAGTGATGACTTCTTTCCATATGCTGACAATATAAATGCTTATTGGACTGGATACTTTACAAGTAGACCAGCCCTCAAAGGATATGTTAGAGTGATGGGTGGCTACTATTTGGCAGCAAGACAATTGGAATTTTTTAAGGGTCGGAGTAAATCAGGGCCCAACACAGAGTATTTGGCTGATGCTTTAGCAATTGCTCAACATCATGATGCAGTTAGTGGCACATCTAAGCAGCATGTGGCTGATGATTATGCAAAACGGCTTTCAATAGGTTATGTGGAGACAGAGAAAGTTGTCGCAAAATCACTGGCTTGCATGACAGAGCCATCACAAGCTGGTTGCAAGAGTGTAGTAACCAAGTTTCAACAGTGTCCCCTTCTGAACATTAGCTATTGTCCGTCATCAGAAGTTCATCTTTCAAAGGGGAAAGACCTGGTTATTGTTGTCTATAATTCTCTGGGATGGAAAAGAAAGGATGTGATAAAGATTCCTGTTGTCAGTGAACATGTCACTGTAAAGGACTCCGCAGGAAAAGAAATTGAATTACAGATTCTACCCCTGCTTAATGAATCCCTGAGCATAAGAAACAACCTCGTTAAGGCATATCTGGGTATATCTCCGAGTGTGACCCCAAGTTATTGGCTTGCATTTTCAGCAACTGTACCACCTCTTGGCTTTAGCACTTACATTGTCTCAAGTGCCAAACAGACAGCTACTACAGAGAGACTGACATTGCACAAGACAGAACTAAGTCAAAACAATGAAATCAAAGTTGGGCCAGGGAACTTGAAACTCATTTATTCTGGAAATGATGGAAAGCTAATTGAATACACAAACAGCAGAAGCTCGGTTAAAGAGTTGGTAGATCAATCATTTAGCTACTACCCTGGAGATGATGGAACCCATGCAAATAAAACTGATTTACAGGCCTCTGGAGCTTATATCTTCCGTCCTAATGGTACATATCCCATTAACTCTGAAGGGGAGGTTCCTCTTACTGTTTTGAGGGGTCCATTGTTAGATGAAGTGCATCAGAAGATCAACTCTTGGATATATCAGGTCACTAGAGTGTACAAGGAAAAAGAGCATGCTGAGATTGAATTTACTGTTGGGCCTATTCCAATTGATGATGGAATTGGCAAAGAGATTGTAACAAAGATTACAACCAGCATGAAGACCAACAAACAATTCTACACAGATTCTAATGGGCGTGATTTTATTGAAAGAATTCGAGACTATAGAAAAGACTGGACCCTGGAAGTAAATCAACCTGTTGCTGGAAATTATTATCCCATTAATCTGGGAATCTATGCGAAAGATAACAACACAGAGATGTCTGTATTAGTAGATAGATCTGTGGGTGGATCCAGCATTGTGGATGGACAATTGGAACTTATGATTCACAGGAGGTTGCTCTATGATGATGCTAGAGGTGTTGGAGAAGCACTAAATGAAACTGTGTGCATTCAAGATGATTGCAAAGGACTAACAGTCACAGGAAAGTACTATCTCAGACTTGATCCTATAGGAGAGGGTGCTAAATGGCGTCGATCATTTGGTCAGGAGATATATTCGCCATTTCTTTTAGCCTTCACAGAACAGGATGGTCATCGGTGGACAAAATCTCATGTCACAACCTTTTCAGGAATGGATCCTTCCTACAGTTTACCAGATAATGTTGCAATAATAACCATCCAGGAGCTAGAAGATGGAAAAGTTCTCTTTCGCCTGGCACATTTATATGAGATTGAAGAGGACAAGGATCTTTCGGTTATGGCAAGTGTAGAACTCAAAAAAGTGTTTGCAAATAAGAAGATAAAGCAAATAACAGAAATGAGCTTATCTGCTAATCAAGGAAGAGCTGAAATGGAGAAGAAGAGACTGGTGTGGAAAGTAGAAGGCTCCTCTGAAGAACCGAAAGCGTTGAGGGGAGGACCTGTTGATCCAACAAAGCTGGTGGTGAATCTTGCTCCAATGGAAATTCGAACATTTACCATAAACTTCTAG

Protein Analysis

1011

Amino Acids

113.8

Weight (kDa)

5.79

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 39 - 351 4.2e-81 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 356 - 447 5.3e-21 Alpha mannosidase middle domain
Laman-like_dom PF21260 550 - 573 5.2e-08 Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C PF07748 602 - 819 1.4e-44 Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2 PF17677 901 - 1006 5.2e-07 Glycosyl hydrolases family 38 C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 92
Acc16I TGCGCA 1 cut(s) 669
AccB1I GGYRCC 1 cut(s) 127
AccB7I CCANNNNNTGG 2 cut(s) 491, 1697
AccI GTMKAC 2 cut(s) 276, 1093
AccII CGCG 1 cut(s) 56
AciI CCGC 6 cut(s) 56, 59, 265, 407, 640, 1551
AclI AACGTT 1 cut(s) 117
AclWI GGATC 8 cut(s) 500, 2385, 2398, 2540, 2672, 2685, 2796, 2963
AcoI YGGCCR 1 cut(s) 1019
AcsI RAATTY 5 cut(s) 923, 1157, 2126, 2253, 3006
AcuI CTGAAG 2 cut(s) 2025, 2955
AcyI GRCGYC 2 cut(s) 89, 2572
AfiI CCNNNNNNNGG 5 cut(s) 491, 544, 1624, 1697, 2303
AflIII ACRYGT 1 cut(s) 1531
AhdI GACNNNNNGTC 1 cut(s) 2516
AjnI CCWGG 7 cut(s) 201, 1458, 1804, 1912, 2280, 2721, 2754
AjuI GAANNNNNNNTTGG 8 cut(s) 107, 139, 1782, 1814, 2118, 2150, 2198, 2230
AleI CACNNNNGTG 1 cut(s) 2093
AloI GAACNNNNNNTCC 2 cut(s) 198, 230
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 5 cut(s) 1301, 1723, 1744, 2253, 2899
AlwI GGATC 8 cut(s) 500, 2385, 2398, 2540, 2672, 2685, 2796, 2963
AlwNI CAGNNNCTG 1 cut(s) 1685
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 6 cut(s) 63, 1019, 1184, 1802, 1953, 2138
ApaI GGGCCC 1 cut(s) 1188
ApeKI GCWGC 4 cut(s) 818, 1004, 1142, 1258
ApoI RAATTY 5 cut(s) 923, 1157, 2126, 2253, 3006
AseI ATTAAT 1 cut(s) 2322
Asp700I GAANNNNTTC 1 cut(s) 2253
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 8 cut(s) 864, 1184, 1185, 1802, 2036, 2138, 2277, 2960
AsuC2I CCSGG 2 cut(s) 105, 106
AsuHPI GGTGA 2 cut(s) 352, 2998
AsuII TTCGAA 1 cut(s) 3010
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 4 cut(s) 864, 2036, 2277, 2960
BaeGI GKGCMC 1 cut(s) 1188
BalI TGGCCA 1 cut(s) 1021
BamHI GGATCC 2 cut(s) 2390, 2677
BanI GGYRCC 1 cut(s) 127
BanII GRGCYC 2 cut(s) 375, 1188
BbsI GAAGAC 2 cut(s) 167, 2207
Bbv12I GWGCWC 1 cut(s) 375
BbvCI CCTCAGC 1 cut(s) 549
BbvI GCAGC 4 cut(s) 805, 1016, 1154, 1270
BceAI ACGGC 1 cut(s) 1301
BciT130I CCWGG 7 cut(s) 203, 1460, 1806, 1914, 2282, 2723, 2756
BciVI GTATCC 1 cut(s) 1073
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 2 cut(s) 105, 106
BcoDI GTCTC 5 cut(s) 1301, 1723, 1744, 2253, 2899
BfaI CTAG 6 cut(s) 656, 711, 2091, 2454, 2729, 3031
BfmI CTRYAG 4 cut(s) 1743, 2263, 2550, 2686
BfuI GTATCC 1 cut(s) 1073
BglII AGATCT 1 cut(s) 2378
BisI GCNGC 6 cut(s) 57, 408, 819, 1005, 1143, 1259
BlsI GCNGC 6 cut(s) 58, 409, 820, 1006, 1144, 1260
BmcAI AGTACT 2 cut(s) 176, 2531
Bme1390I CCNGG 9 cut(s) 105, 106, 203, 1460, 1806, 1914, 2282, 2723, 2756
Bme18I GGWCC 4 cut(s) 864, 2036, 2277, 2960
BmeRI GACNNNNNGTC 1 cut(s) 2516
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 8 cut(s) 864, 1184, 1185, 1802, 2036, 2138, 2277, 2960
BmrFI CCNGG 9 cut(s) 105, 106, 203, 1460, 1806, 1914, 2282, 2723, 2756
BmsI GCATC 7 cut(s) 460, 730, 982, 1198, 1225, 2065, 2440
BpiI GAAGAC 2 cut(s) 167, 2207
BpmI CTGGAG 2 cut(s) 1935, 1980
Bpu10I CCTNAGC 2 cut(s) 549, 1601
Bpu14I TTCGAA 1 cut(s) 3010
BpuEI CTTGAG 3 cut(s) 389, 647, 1705
BpuMI CCSGG 2 cut(s) 105, 106
BsaBI GATNNNNATC 1 cut(s) 2634
BsaHI GRCGYC 2 cut(s) 89, 2572
BsaJI CCNNGG 7 cut(s) 103, 104, 202, 867, 1805, 1912, 2280
BsaWI WCCGGW 2 cut(s) 22, 359
BsaXI ACNNNNNCTCC 8 cut(s) 53, 83, 170, 198, 200, 228, 2949, 2979
Bsc4I CCNNNNNNNGG 5 cut(s) 491, 544, 1624, 1697, 2303
Bse1I ACTGG 5 cut(s) 172, 1081, 1338, 2279, 2913
Bse3DI GCAATG 3 cut(s) 245, 828, 1757
Bse8I GATNNNNATC 1 cut(s) 2634
BseBI CCWGG 7 cut(s) 203, 1460, 1806, 1914, 2282, 2723, 2756
BseDI CCNNGG 7 cut(s) 103, 104, 202, 867, 1805, 1912, 2280
BseGI GGATG 8 cut(s) 158, 198, 393, 1493, 1507, 2410, 2635, 2718
BseJI GATNNNNATC 1 cut(s) 2634
BseLI CCNNNNNNNGG 5 cut(s) 491, 544, 1624, 1697, 2303
BseMI GCAATG 3 cut(s) 245, 828, 1757
BseMII CTCAG 4 cut(s) 540, 1592, 2109, 2551
BseNI ACTGG 5 cut(s) 172, 1081, 1338, 2279, 2913
BseRI GAGGAG 2 cut(s) 338, 2920
BseSI GKGCMC 1 cut(s) 1188
BseXI GCAGC 4 cut(s) 805, 1016, 1154, 1270
BsgI GTGCAG 1 cut(s) 548
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 6 cut(s) 65, 1021, 1186, 1804, 1955, 2140
BshNI GGYRCC 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 4 cut(s) 23, 105, 242, 360
BslFI GGGAC 2 cut(s) 86, 1383
BslI CCNNNNNNNGG 5 cut(s) 491, 544, 1624, 1697, 2303
BsmAI GTCTC 5 cut(s) 1301, 1723, 1744, 2253, 2899
BsmFI GGGAC 2 cut(s) 86, 1383
BsmI GAATGC 1 cut(s) 2490
BsnI GGCC 6 cut(s) 65, 1021, 1186, 1804, 1955, 2140
BsoBI CYCGRG 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 3010
Bsp120I GGGCCC 1 cut(s) 1184
Bsp1286I GDGCHC 2 cut(s) 375, 1188
Bsp1407I TGTACA 2 cut(s) 754, 2097
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 6 cut(s) 56, 59, 265, 407, 640, 1551
BspANI GGCC 6 cut(s) 65, 1021, 1186, 1804, 1955, 2140
BspCNI CTCAG 4 cut(s) 541, 1593, 2110, 2550
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 1 cut(s) 1231
BspPI GGATC 8 cut(s) 500, 2385, 2398, 2540, 2672, 2685, 2796, 2963
BspQI GCTCTTC 1 cut(s) 2878
BspT104I TTCGAA 1 cut(s) 3010
BspT107I GGYRCC 1 cut(s) 127
BsrDI GCAATG 3 cut(s) 245, 828, 1757
BsrGI TGTACA 2 cut(s) 754, 2097
BsrI ACTGG 5 cut(s) 172, 1081, 1338, 2279, 2913
BssECI CCNNGG 7 cut(s) 103, 104, 202, 867, 1805, 1912, 2280
BssNAI GTATAC 1 cut(s) 277
BssNI GRCGYC 2 cut(s) 89, 2572
BssT1I CCWWGG 1 cut(s) 867
Bst1107I GTATAC 1 cut(s) 277
Bst2UI CCWGG 7 cut(s) 203, 1460, 1806, 1914, 2282, 2723, 2756
Bst6I CTCTTC 3 cut(s) 2772, 2878, 2897
BstACI GRCGYC 2 cut(s) 89, 2572
BstAPI GCANNNNNTGC 1 cut(s) 692
BstAUI TGTACA 2 cut(s) 754, 2097
BstBI TTCGAA 1 cut(s) 3010
BstC8I GCNNGC 7 cut(s) 252, 288, 497, 531, 1339, 1671, 2115
BstDEI CTNAG 7 cut(s) 549, 648, 1254, 1601, 1775, 2118, 2537
BstDSI CCRYGG 1 cut(s) 867
BstENI CCTNNNNNAGG 1 cut(s) 1622
BstF5I GGATG 8 cut(s) 158, 198, 393, 1493, 1507, 2410, 2635, 2718
BstFNI CGCG 1 cut(s) 56
BstHHI GCGC 1 cut(s) 670
BstMAI GTCTC 5 cut(s) 1301, 1723, 1744, 2253, 2899
BstMWI GCNNNNNNNGC 9 cut(s) 11, 62, 232, 251, 530, 692, 1001, 1679, 1961
BstNI CCWGG 7 cut(s) 203, 1460, 1806, 1914, 2282, 2723, 2756
BstNSI RCATGY 3 cut(s) 1265, 1535, 2117
BstSCI CCNGG 9 cut(s) 103, 104, 201, 1458, 1804, 1912, 2280, 2721, 2754
BstSFI CTRYAG 4 cut(s) 1743, 2263, 2550, 2686
BstSLI GKGCMC 1 cut(s) 1188
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 4 cut(s) 805, 1016, 1154, 1270
BstV2I GAAGAC 2 cut(s) 167, 2207
BstX2I RGATCY 4 cut(s) 2378, 2390, 2677, 2788
BstXI CCANNNNNNTGG 3 cut(s) 1665, 2326, 2401
BstYI RGATCY 4 cut(s) 2378, 2390, 2677, 2788
BstZ17I GTATAC 1 cut(s) 277
BsuI GTATCC 1 cut(s) 1073
BsuRI GGCC 6 cut(s) 65, 1021, 1186, 1804, 1955, 2140
BtgI CCRYGG 1 cut(s) 867
BtsCI GGATG 8 cut(s) 158, 198, 393, 1493, 1507, 2410, 2635, 2718
BtsIMutI CAGTG 4 cut(s) 1331, 1400, 1531, 1536
Cac8I GCNNGC 7 cut(s) 252, 288, 497, 531, 1339, 1671, 2115
CaiI CAGNNNCTG 1 cut(s) 1685
CciI TCATGA 1 cut(s) 1231
CfoI GCGC 1 cut(s) 670
Cfr13I GGNCC 8 cut(s) 864, 1184, 1185, 1802, 2036, 2138, 2277, 2960
Cfr9I CCCGGG 1 cut(s) 104
CseI GACGC 1 cut(s) 2561
CsiI ACCWGGT 1 cut(s) 1458
CspCI CAANNNNNGTGG 4 cut(s) 287, 322, 2964, 2999
DdeI CTNAG 7 cut(s) 549, 648, 1254, 1601, 1775, 2118, 2537
DriI GACNNNNNGTC 1 cut(s) 2516
EaeI YGGCCR 1 cut(s) 1019
Eam1104I CTCTTC 3 cut(s) 2772, 2878, 2897
Eam1105I GACNNNNNGTC 1 cut(s) 2516
EarI CTCTTC 3 cut(s) 2772, 2878, 2897
EciI GGCGGA 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 373
Eco130I CCWWGG 1 cut(s) 867
Eco147I AGGCCT 1 cut(s) 1955
Eco24I GRGCYC 2 cut(s) 375, 1188
Eco47I GGWCC 4 cut(s) 864, 2036, 2277, 2960
Eco53kI GAGCTC 1 cut(s) 373
Eco57I CTGAAG 2 cut(s) 2025, 2955
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 373
EcoNI CCTNNNNNAGG 1 cut(s) 1622
EcoO109I RGGNCCY 2 cut(s) 1184, 2960
EcoRI GAATTC 1 cut(s) 2253
EcoRII CCWGG 7 cut(s) 201, 1458, 1804, 1912, 2280, 2721, 2754
EcoT14I CCWWGG 1 cut(s) 867
EcoT38I GRGCYC 2 cut(s) 375, 1188
ErhI CCWWGG 1 cut(s) 867
FalI AAGNNNNNCTT 2 cut(s) 1425, 1457
FaqI GGGAC 2 cut(s) 86, 1383
FauI CCCGC 1 cut(s) 633
FauNDI CATATG 1 cut(s) 1048
FbaI TGATCA 1 cut(s) 433
FblI GTMKAC 2 cut(s) 276, 1093
Fnu4HI GCNGC 6 cut(s) 57, 408, 819, 1005, 1143, 1259
FokI GGATG 8 cut(s) 165, 185, 400, 1500, 1514, 2417, 2642, 2705
FriOI GRGCYC 2 cut(s) 375, 1188
Fsp4HI GCNGC 6 cut(s) 57, 408, 819, 1005, 1143, 1259
FspBI CTAG 6 cut(s) 656, 711, 2091, 2454, 2729, 3031
FspI TGCGCA 1 cut(s) 669
GlaI GCGC 1 cut(s) 669
GluI GCNGC 6 cut(s) 57, 408, 819, 1005, 1143, 1259
GsuI CTGGAG 2 cut(s) 1935, 1980
HaeIII GGCC 6 cut(s) 65, 1021, 1186, 1804, 1955, 2140
HapII CCGG 4 cut(s) 23, 105, 242, 360
HgaI GACGC 1 cut(s) 2561
HhaI GCGC 1 cut(s) 670
Hin1I GRCGYC 2 cut(s) 89, 2572
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HincII GTYRAC 3 cut(s) 169, 365, 616
HindII GTYRAC 3 cut(s) 169, 365, 616
HpaII CCGG 4 cut(s) 23, 105, 242, 360
HphI GGTGA 2 cut(s) 352, 2998
Hpy188III TCNNGA 9 cut(s) 944, 1027, 1232, 1830, 1959, 2258, 2495, 2588, 2670
Hpy99I CGWCG 6 cut(s) 91, 94, 146, 149, 284, 2577
HpyAV CCTTC 6 cut(s) 337, 1412, 2000, 2626, 2691, 2918
HpyCH4IV ACGT 6 cut(s) 89, 117, 147, 180, 222, 279
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 62, 232, 251, 530, 692, 1001, 1679, 1961
HpyF3I CTNAG 7 cut(s) 549, 648, 1254, 1601, 1775, 2118, 2537
HpySE526I ACGT 6 cut(s) 89, 117, 147, 180, 222, 279
Hsp92I GRCGYC 2 cut(s) 89, 2572
HspAI GCGC 1 cut(s) 668
Ksp22I TGATCA 1 cut(s) 433
LguI GCTCTTC 1 cut(s) 2878
LmnI GCTCC 9 cut(s) 191, 206, 346, 362, 415, 1961, 2725, 2933, 3001
Lsp1109I GCAGC 4 cut(s) 805, 1016, 1154, 1270
LweI GCATC 7 cut(s) 460, 730, 982, 1198, 1225, 2065, 2440
MabI ACCWGGT 1 cut(s) 1458
MaeI CTAG 6 cut(s) 656, 711, 2091, 2454, 2729, 3031
MaeII ACGT 6 cut(s) 89, 117, 147, 180, 222, 279
MaeIII GTNAC 9 cut(s) 478, 825, 1378, 1534, 1651, 2086, 2176, 2518, 2656
MfeI CAATTG 4 cut(s) 1151, 1218, 2148, 2411
MflI RGATCY 4 cut(s) 2378, 2390, 2677, 2788
MhlI GDGCHC 2 cut(s) 375, 1188
MlsI TGGCCA 1 cut(s) 1021
MluNI TGGCCA 1 cut(s) 1021
MlyI GAGTC 6 cut(s) 436, 475, 557, 661, 1022, 1541
MmeI TCCRAC 5 cut(s) 130, 535, 1151, 2443, 2996
Mox20I TGGCCA 1 cut(s) 1021
MroXI GAANNNNTTC 1 cut(s) 2253
MscI TGGCCA 1 cut(s) 1021
MslI CAYNNNNRTG 5 cut(s) 425, 1233, 2093, 2357, 2766
Msp20I TGGCCA 1 cut(s) 1021
MspI CCGG 4 cut(s) 23, 105, 242, 360
MspR9I CCNGG 9 cut(s) 105, 106, 203, 1460, 1806, 1914, 2282, 2723, 2756
MunI CAATTG 4 cut(s) 1151, 1218, 2148, 2411
Mva1269I GAATGC 1 cut(s) 2490
MvaI CCWGG 7 cut(s) 203, 1460, 1806, 1914, 2282, 2723, 2756
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 9 cut(s) 11, 62, 232, 251, 530, 692, 1001, 1679, 1961
NciI CCSGG 2 cut(s) 105, 106
NcoI CCATGG 1 cut(s) 867
NdeI CATATG 1 cut(s) 1048
NmuCI GTSAC 6 cut(s) 478, 1534, 1651, 2086, 2518, 2656
NsbI TGCGCA 1 cut(s) 669
NspI RCATGY 3 cut(s) 1265, 1535, 2117
NspV TTCGAA 1 cut(s) 3010
OliI CACNNNNGTG 1 cut(s) 2093
PaeI GCATGC 1 cut(s) 2117
PagI TCATGA 1 cut(s) 1231
PceI AGGCCT 1 cut(s) 1955
PciI ACATGT 1 cut(s) 1531
PciSI GCTCTTC 1 cut(s) 2878
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PctI GAATGC 1 cut(s) 2490
PdmI GAANNNNTTC 1 cut(s) 2253
PfeI GAWTC 9 cut(s) 230, 238, 1513, 1576, 1595, 2225, 2331, 2425, 2989
PflMI CCANNNNNTGG 2 cut(s) 491, 1697
PfoI TCCNGGA 1 cut(s) 2721
PkrI GCNGC 6 cut(s) 58, 409, 820, 1006, 1144, 1260
PleI GAGTC 6 cut(s) 436, 475, 557, 660, 1021, 1541
PpsI GAGTC 6 cut(s) 436, 475, 557, 660, 1021, 1541
PpuMI RGGWCCY 1 cut(s) 2960
PscI ACATGT 1 cut(s) 1531
PshBI ATTAAT 1 cut(s) 2322
Psp124BI GAGCTC 1 cut(s) 375
Psp1406I AACGTT 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 2960
Psp6I CCWGG 7 cut(s) 201, 1458, 1804, 1912, 2280, 2721, 2754
PspGI CCWGG 7 cut(s) 201, 1458, 1804, 1912, 2280, 2721, 2754
PspOMI GGGCCC 1 cut(s) 1184
PspPI GGNCC 8 cut(s) 864, 1184, 1185, 1802, 2036, 2138, 2277, 2960
PspPPI RGGWCCY 1 cut(s) 2960
PstNI CAGNNNCTG 1 cut(s) 1685
PsuI RGATCY 4 cut(s) 2378, 2390, 2677, 2788
RseI CAYNNNNRTG 5 cut(s) 425, 1233, 2093, 2357, 2766
SacI GAGCTC 1 cut(s) 375
SapI GCTCTTC 1 cut(s) 2878
SatI GCNGC 6 cut(s) 57, 408, 819, 1005, 1143, 1259
Sau96I GGNCC 8 cut(s) 864, 1184, 1185, 1802, 2036, 2138, 2277, 2960
ScaI AGTACT 2 cut(s) 176, 2531
SchI GAGTC 6 cut(s) 436, 475, 557, 661, 1022, 1541
ScrFI CCNGG 9 cut(s) 105, 106, 203, 1460, 1806, 1914, 2282, 2723, 2756
SduI GDGCHC 2 cut(s) 375, 1188
SexAI ACCWGGT 1 cut(s) 1458
SfaNI GCATC 7 cut(s) 460, 730, 982, 1198, 1225, 2065, 2440
SfcI CTRYAG 4 cut(s) 1743, 2263, 2550, 2686
SfuI TTCGAA 1 cut(s) 3010
SinI GGWCC 4 cut(s) 864, 2036, 2277, 2960
SmaI CCCGGG 1 cut(s) 106
SmiMI CAYNNNNRTG 5 cut(s) 425, 1233, 2093, 2357, 2766
SmlI CTYRAG 3 cut(s) 368, 626, 1720
SmoI CTYRAG 3 cut(s) 368, 626, 1720
SphI GCATGC 1 cut(s) 2117
SseBI AGGCCT 1 cut(s) 1955
SsiI CCGC 6 cut(s) 56, 59, 265, 407, 640, 1551
SspMI CTAG 6 cut(s) 656, 711, 2091, 2454, 2729, 3031
SstI GAGCTC 1 cut(s) 375
StuI AGGCCT 1 cut(s) 1955
StyD4I CCNGG 9 cut(s) 103, 104, 201, 1458, 1804, 1912, 2280, 2721, 2754
StyI CCWWGG 1 cut(s) 867
TaiI ACGT 6 cut(s) 92, 120, 150, 183, 225, 282
TaqI TCGA 4 cut(s) 282, 2257, 2575, 3010
TatI WGTACW 7 cut(s) 69, 78, 174, 754, 966, 2097, 2529
TauI GCSGC 2 cut(s) 59, 410
TfiI GAWTC 9 cut(s) 230, 238, 1513, 1576, 1595, 2225, 2331, 2425, 2989
TscAI CASTG 4 cut(s) 1338, 1400, 1531, 1543
TseFI GTSAC 6 cut(s) 478, 1534, 1651, 2086, 2518, 2656
TseI GCWGC 4 cut(s) 818, 1004, 1142, 1258
Tsp45I GTSAC 6 cut(s) 478, 1534, 1651, 2086, 2518, 2656
TspGWI ACGGA 3 cut(s) 498, 1413, 1964
TspMI CCCGGG 1 cut(s) 104
TspRI CASTG 4 cut(s) 1338, 1400, 1531, 1543
Van91I CCANNNNNTGG 2 cut(s) 491, 1697
VpaK11BI GGWCC 4 cut(s) 864, 2036, 2277, 2960
VspI ATTAAT 1 cut(s) 2322
XagI CCTNNNNNAGG 1 cut(s) 1622
XapI RAATTY 5 cut(s) 923, 1157, 2126, 2253, 3006
XceI RCATGY 3 cut(s) 1265, 1535, 2117
XcmI CCANNNNNNNNNTGG 3 cut(s) 146, 179, 492
XmaI CCCGGG 1 cut(s) 104
XmiI GTMKAC 2 cut(s) 276, 1093
XmnI GAANNNNTTC 1 cut(s) 2253
XspI CTAG 6 cut(s) 656, 711, 2091, 2454, 2729, 3031
ZraI GACGTC 1 cut(s) 90
ZrmI AGTACT 2 cut(s) 176, 2531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.